Periodicity of Coliform Bacteria in an Aquatic Environment

1999 ◽  
Vol 40 (7) ◽  
pp. 151-157 ◽  
Author(s):  
U. S. Bagde ◽  
A. K. Rangari
Keyword(s):  
Lake Water ◽  
Water Body ◽  
Seasonal Variations ◽  
Bacterial Population ◽  
Winter Season ◽  
Total Coliform ◽  
Seasonal Distribution ◽  
Point Of View ◽  
Coliform Bacteria ◽  
Drinking Purposes

The present study deals with enumeration and seasonal distribution of total and faecal coliform bacteria, the indicators of microorganisms causing faecal pollution of the aquatic ecosystem by a multiple tube technique. A lake called Upvan lake of Thane city, India was investigated during 1996-97 for the purpose. Bacterial population varied with seasonal variations in the water body. Bacterial counts increased during summer months and lowered during winter months. Highest coliform counts were reported in monsoon and lowest in winter season. Samples collected from different sites of the lake showed wide variations in the counts of bacteria. Positive correlation was established between faecal and total coliform. Bacteriological analysis of lake water indicated that water was polluted by faecal contaminants to the extent that it was unportable for drinking purposes and unsuitable from the recreation point of view and hence needed thorough impoundment.

scholarly journals STUDY OF PHYSICOCHEMICAL CHARACTERISTICS OF BIRD VALLEY’S QUARRY WATER PCMC, MAHARASHTRA

2021 ◽  
Vol 6 (2) ◽  
pp. 18-24
Author(s):  
Shashikala Kokcha ◽  
Harsha Chatrath
Keyword(s):  
Seasonal Variations ◽  
Water Samples ◽  
Physicochemical Characteristics ◽  
Point Of View ◽  
Present Condition ◽  
Chemical Parameters ◽  
Standard Methods ◽  
Drinking Purposes ◽  
Physico Chemical ◽  
Methods And Techniques

Purpose of the study: The purpose of this study is to make sure that the Bird valley’s quarry water is suitable for drinking purposes or not and to monitor the seasonal variations in the physico-chemical parameters of this quarry water. Methodology: Water samples from the quarry were collected in clean and sterilized polyethylene bottles. Water samples were collected from different points and mixed together to get an integrated sample. Some of the selected physico-chemical parameters of the quarry water have been analyzed. Results were compared with standard limits of IS: 10500-2012. All the parameters were analyzed in the laboratory by using standard methods and techniques. Main Findings: As per the obtained results this quarry water contains a very large number of Coliforms detected in the months of September and December and in June month Coliform count was 33 CFU/ml. This overall result for coliform is making this quarry water unfit for domestic purposes. Applications of this study: This study helps us to understand the current condition of this quarry water and also enables us to know whether the quarry water is fit for drinking purposes. It also enables us to know whether this quarry water can be used for domestic purposes after the treatment. Novelty/Originality of this study: Bird valley’s quarry water has not been analyzed from this point of view till date. This study will help us to understand the present condition of the water.

scholarly journals Comparative study of some indicator bacteria in cows and buffaloes raw milk in Al-Diwaniyah Province

2012 ◽  
Vol 11 (3) ◽  
pp. 46
Author(s):  
K. H. Abass
Keyword(s):  
Winter Season ◽  
Bacterial Count ◽  
Raw Milk ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Winter Period ◽  
City Center ◽  
Summer Period ◽  
Milk Samples ◽  
Significant Difference

The present study was carried out in AL-Diwaniyah city and it's different districts whichincludes (city center, Afak district and their regions, AL-hamza district and their regions, ALShamiadistrict and their regions and AL-Daghara regions and its villages) during a periodNovember 2010 to July 2010. A total of samples used in this study were 120 raw milksamples collected includes 60 samples for each species for (cows and buffaloes) for twodifferent season of the year (Winter and Summer), first start from (beginning November 2009 toend of the January 2010) ,and the second start from (beginning May to July 2010) The resultsof this study revealed the rising the averages of microbes contaminated raw milk samples ineach species of animals included in this study,where raw milk samples for cows recorded210 4.2х CFU/ml , buffaloes 214 12х CFU/ml,The highest averages of total Coliform bacterialcount were found in raw milk samples of cows 214 4.3х CFU/ml and less averages in rawsamples of buffaloes 214 6.1х CFU/ml . While averages of Staphylococcus aureas were highin raw milk samples of buffaloes 0.49 х216CFU/ml and less in raw milk samples of cows0. 214 61х CFU/ ml.While the averages of Streptococcus spp. were 0.37х 216CFU/ml, 0.38 21х6 CFU/ml, for cows and buffaloes respectively. Seasons of the year had an effect on average oftotal bacterial count studied for each sample of raw milk for all species of animals includedin this study.Where shown rising averages of microbes numbers during the summer period(May, Jun, and July), while there are decreasing in an average of microbes during the winter period(November, December, and January).Summer season recorded significant difference ascompared with winter season but averages of total coliform bacteria had no significantdifference between the two seasons.

Pengaruh Suhu Penyimpanan terhadap Organoleptik, Derajat Keasaman dan Pertumbuhan Bakteri Coliform pada Susu Pasteurisasi

2016 ◽  
Vol 10 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Agustina Arianita Cahyaningtyas ◽  
Wiwik Pudjiastuti ◽  
Ilham Ramdhan
Keyword(s):  
Storage Temperature ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Plate Count ◽  
Pasteurized Milk ◽  
Initial Analysis ◽  
Total Plate Count ◽  
Plate Count Method ◽  
Pathogenic Microbes ◽  
Total Coliform Bacteria

One attempt to reduce the number of pathogenic microbes in milk is through the pasteurization process. This research aims to determine the effect of storage temperature on the organoleptic, acidity (pH) and growth of coliform bacteria in pasteurized milk. Pasteurized milk is stored at the varies of temperature  4°C (observed for 14 days), 10°C-15°C (observed for 14 days) and 25°C-27°C (observed for 22 hours), as well as also conducted an initial analysis pasteurized milk. The parameters were observed among other organoleptic (smell, taste, color, texture), pH and total coliform bacteria. Testing acidity using pH paper, while the growth of coliform bacteria testing done using Total Plate Count method based on ISO 2897 in 2008. The results of this study indicate that storage at 4°C for 14 days, organoleptic pasteurized milk is still good until the day ke- 8, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 3100x101 CFU / ml. Storage at 10°C-15°C for 14 days, organoleptic pasteurized milk is still good until the 6th day, the pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 5729x101 CFU / ml. Storage at 25°C-27°C for 22 days, organoleptic pasteurized milk is still good until the 9th, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 4.3 x106 CFU / ml.ABSTRAKSalah satu usaha untuk mengurangi jumlah mikroba patogen pada susu adalah melalui proses pasteurisasi. Penelitian ini bertujuan untuk mengetahui pengaruh suhu penyimpanan terhadap organoleptik, derajat keasaman (pH) dan pertumbuhan bakteri Coliform pada susu pasteurisasi. Susu pasteurisasi disimpan pada suhu yang bervariasi yaitu suhu 4°C (diamati selama 14 hari), suhu 10°C-15°C (diamati selama 14 hari) dan suhu 25°C-27°C (diamati selama 22 jam), serta dilakukan pula analisa awal susu pasteurisasi. Parameter yang diamati antara lain organoleptik (bau, rasa, warna, tekstur), pH dan jumlah bakteri Coliform. Pengujian derajat keasaman menggunakan kertas pH, sedangkan pengujian pertumbuhan bakteri Coliform dilakukan dengan menggunakan metode Total Plate Count berdasarkan SNI 2897 Tahun 2008. Hasil penelitian ini menunjukkan bahwa penyimpanan pada suhu 4°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai dengan hari ke-8, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 3100x101 Cfu/ml. Penyimpanan pada suhu 10°C-15°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai hari ke-6, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 5729x101 Cfu/ml. Penyimpanan pada suhu 25°C-27°C selama 22 hari, organoleptik susu pasteurisasi masih baik sampai jam ke-9, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 4,3 x106 Cfu/ml.Kata kunci : bakteri coliform, derajat keasaman, suhu penyimpanan, organoleptik, susu pasteurisasi

MICROBES PARAMETERS OF WATER QUALITY FOR AQUACULTURE ON PARI ISLAND WATERS

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Lies Indah Sutiknowati
Keyword(s):  
Pathogenic Bacteria ◽  
Heterotrophic Bacteria ◽  
Total Coliform ◽  
Total Cell ◽  
Epifluorescence Microscopy ◽  
Coliform Bacteria ◽  
Total Density ◽  
Shellfish Aquaculture ◽  
Microbiological Parameters ◽  
Phosphate Nitrate

The objective of this research was to evaluate waters quality in Pari island waters for aquaculture purpose based on bacteriological information conducted in Mei and September 2011. Microbiological parameters analyzed were total density of bacteria for coliforms, E.coli, pathogenic, heterotrophic, halotoleran, phosphate-nitrate-ammonia breaker, and total cells. Method to analyze coliform bacteria was filtration, identification of pathogenic bacteria using biochemical test, density analises for heterotrophic bacteria, analises for phosphate-nitrate-ammonia breaker bacteria using pour plate, and total cell using Acridine Orange Epifluorescence Microscopy. Results showed that the abundance of total coliform cell was about 1000-7000 colony forming unit (cfu)/100 ml. The abundance of heterotrophic, halotolerant, phosphate-nitrate-ammonia bacteria in seawater was (3.6-4.3)x105 cfu/ml, (1.1-1.3)x105 cfu/ml, (0.5-3.44)x103cfu/ml; and (3.6-6.7)x105 cfu/ml, (1.6-2.7)x105 cfu/ml, (0.6-5.22)x103 cfu/ml in sediment, respectively. The total cell of bacteria was (0.05-2.1)x107cells/ml. The dog-conch (Strombus turturella) and blood-clamps (Anadara granosa) can survive in Pari Island and there was a significant increase in sea grass litter with growth average of 0.67 mm/day and 0.90 mm/day. During snails and clamps growth, there were found several genus of pathogenic bacteria such as Salmonella, Vibrio, Aeromonas, Pseudomonas, Citrobacter, Proteus, Shigella, Hafnia, and Yersinia. The results showed that Pari island waters was suitable for developing shellfish aquaculture dog conch and blood clamps. Keywords: bacteria, parameter, shellfish, aquaculture.

Seasonal Variations of 2,4-D in Buckhorn Lake

1985 ◽  
Vol 20 (1) ◽  
pp. 106-117 ◽  
Author(s):  
E. Nagy ◽  
D.S. Painter
Keyword(s):  
Lake Water ◽  
Seasonal Variations ◽  
Water And Sediment ◽  
Streamflow Data ◽  
Land Runoff

Abstract A five month survey of 2,4-D in Buckhorn Lake water and sediment, combined with streamflow data, produced estimates of the chemical in the lake. The peak concentrations in the water alone were significantly higher than the documented uses would permit. Two distinct concentration peaks were shown by the chemical: one during the June milfoil control season, and one in late August, indicating a release of 2,4-D from the collapsed and decaying milfoil beds. The observed rates of disappearance of the chemical agreed with reported values, showing a limited persistence in the lake. The presence of 2,4-D in the spring sediments was attributed to land runoff.

Bacterial die-away rates in red sea waters

1995 ◽  
Vol 32 (2) ◽  
pp. 45-52 ◽  
Author(s):  
H. Z. Sarikaya ◽  
A. M. Saatçi
Keyword(s):  
Solar Radiation ◽  
Decay Rate ◽  
Sea Water ◽  
Membrane Filter ◽  
Linear Regression Analysis ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Decay Rate Constant ◽  
Solar Intensity ◽  
Log Normal

Total coliform bacteria have been chosen as the indicator organism. Coliform die-away experiments have been carried out in unpolluted sea water samples collected at about 100 m off the coastline and under controlled environmental conditions. The samples were transformed into one litre clean glass beakers which were kept at constant temperature and were exposed to the solar radiation. The membrane filter technique was used for the coliform analysis. The temperature ranged from 20 to 40° C and the dilution ratios ranged from 1/50 to 1/200. Coliform decay rate in the light has been expressed as the summation of the coliform decay rate in the dark and the decay rate due to solar radiation. The solar radiation required for 90 percent coliform removal has been found to range from 17 cal/cm2 to 40 cal/cm2 within the temperature range of 25 to 30° C. Applying the linear regression analysis two different equations have been given for the high (I>10 cal/cm2.hour) and low solar intensity ranges in order to determine the coliform decay rate constant as a function of the solar intensity. T-90 values in the light have been found to follow log-normal distribution with a median T-90 value of 32 minutes. The corresponding T-90 values in the dark were found to be 70-80 times longer. Coliform decay rate in the dark has been correlated with the temperature.

scholarly journals Comparative Tissue Responses of Marine Mollusks on Seasonal Changes in the Northern Adriatic Sea

2021 ◽  
Vol 11 (6) ◽  
pp. 2874
Author(s):  
Natalija Topić Popović ◽  
Martina Krbavčić ◽  
Josip Barišić ◽  
Blanka Beer Ljubić ◽  
Ivančica Strunjak-Perović ◽  
...  
Keyword(s):  
Stress Responses ◽  
Seasonal Variations ◽  
Antioxidant Defense ◽  
Winter Season ◽  
Northern Adriatic ◽  
Gonadal Maturation ◽  
Marine Mollusks ◽  
Significant Difference ◽  
Tissue Responses ◽  
Antioxidant Defense Systems

In the shallow Northern Adriatic, marine mollusks are affected by bottom trawling and seafood disturbance. Seasonal oscillations of oceanographic factors additionally influence their physiology, stress responses and survival. Tissue responses to seasonal variations in green ormer (Haliotis tuberculata L.) and Mediterranean scallop (Pecten jacobaeus L.) in the Northern Adriatic have not been reported. Hence, their biochemical and antioxidant defense properties over seasons were studied and the microanatomical structure of their tissue was correlated with function. Histological analysis of gonads revealed two peaks of gonadal maturation and spawning during the spring/summer period and winter season for scallops, and one peak during the fall for ormers. The gonadal maturation of both species was correlated with their seasonal variations of metabolic demands and antioxidant capacity. The lipid vacuoles of tubuloacinar terminations in the digestive gland differed between the two species; in scallop they are several-fold larger in size and number. Low temperatures in winter contributed to a decline in enzymatic antioxidant defense in scallop tissues, having lower superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity, and higher concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant status (TAS). In ormers, winter induced lower TAS, TBARS, SOD and GPx concentrations. The significant difference of winter TAS and TBARS levels between ormers and scallops was correlated with variations in their reproductive cycles, as well as in antioxidant defense systems. The most important factor for stress-related parameters for both species in this work was found to be the season-induced temperature change.

scholarly journals Improvement of waste water quality by application of mixed algal inocula

2018 ◽  
Vol 53 (1) ◽  
pp. 77-82
Author(s):  
MA Riaz ◽  
B Ijaz ◽  
A Riaz ◽  
M Amjad
Keyword(s):  
Waste Water ◽  
Experimental Treatment ◽  
Total Coliform ◽  
Post Treatment ◽  
Coliform Bacteria ◽  
Physiochemical Parameters ◽  
Pollutant Concentrations ◽  
Pre Treatment ◽  
Wastewater Samples ◽  
The One

Present study aim to investigate application of microalgae for improvement of physiochemical parameters of waste water and improvement of treatment process through reducing the pollutant concentrations including coliform bacteria through their ability to uptake nutrients. Aquatic cultures were conducted in container with dimensions of 1.5×3sq ft. and wastewater samples 5 Litre samples were collected in plastic cans from Sukhnar drain and algal samples were collected from Bhaini Sigyain Village near Ravi Siphon, Lahore. The one experimental treatment lasted for 10 days to make 50% 5 liter water added concentration and 200g of inocula of algae were grown in wastewater container for experiments to optimize at room temperature as local outdoor cultures conditions for wastewater treatment. Total experiment repeated thrice and completed in 30 days. Results show that treatments improved the physiochemical parameter after week. Turbidity (NTU), total suspended solids (mg/L), total dissolved solids (mg/L), nitrate (mg/L), phosphate (mg/L) decrease (P < 0.05) after treatment. However, pH not significantly decreases (P≥ 0.05) pre-treatment values 6.56±0.05 (mg/L) and post-treatment 6.53± 0.057(mg/L). Maximum total coliform disinfection (P < 0.05) was indicated with 200 g of algae was pre-treatment 1602± 6.8 (MPN) and post treatment 1447±39.5 (MPN) observed.Bangladesh J. Sci. Ind. Res.53(1), 77-82, 2018

scholarly journals Use of ceramic pot filter (CPF) technology under pressure in an in-line pumping system

2017 ◽  
Vol 18 (3) ◽  
pp. 843-852
Author(s):  
Travis D. Gardner ◽  
Joe D. Guggenberger
Keyword(s):  
Water Treatment ◽  
Low Cost ◽  
Total Coliform ◽  
Fecal Coliforms ◽  
Coliform Bacteria ◽  
Limiting Factor ◽  
Water Production ◽  
Pumping System ◽  
Point Of Use ◽  
Logarithmic Reduction

Abstract Ceramic pot filters (CPFs) are an effective point of use water treatment device in developing nations due to their low cost and effectiveness. CPFs are gravity fed, typically making water production a major limiting factor to a CPF's lifetime and acceptability. Directly connecting CPFs to in-line pumping systems or systems with an elevated storage tank would allow filter usage for constant water treatment at increased pressures, increasing the quantity of treated water. Ceramic disks were manufactured for testing in a specially designed housing apparatus. Filters of varying thicknesses and clay to sawdust mass ratios were manufactured to fit tightly. Flowrate and microbiological removal efficacy (logarithmic reduction value (LRV)) were determined over the testing period at various pressures. Flowrate values ranged from 2.44 to 9.04 L per hour, significantly higher than traditional CPF technology. LRVs ranged from 1.1 to 2.0, lower than traditional CPF technology but still effective at removing most Escherichia coli and total coliform bacteria. Filters proved effective at removing total and fecal coliforms at pressures less than 70 kilopascals. The optimum filter had a thickness of 3.2 cm and clay to sawdust ratio of 6:1 by mass. Filters proved to be ineffective if flowrates were above 5 L/h.

scholarly journals Analysis of Water Quality and River Waters Microbology for Manifestation of Food Safety

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Rizky Muliani Dwi Ujianti ◽  
Althesa Androva
Keyword(s):  
Water Quality ◽  
Food Security ◽  
Quality Management ◽  
Water Quality Management ◽  
Quality Standard ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Domestic Waste ◽  
Total Coliform Bacteria

 Abstract. Banjir Kanal Barat is a river in the Garang watershed, Semarang City, Central Java, Indonesia. Its function is as a source of water for the community. The level of pollution in this river is already high. The purpose of this study is to provide advice to governments, communities and related stakeholders to realize integrated river management, and fisheries-based food security is achieved. This research method is: analyzing the water quality of the Banjir Kanal Barat river, and analyzing the amount of faecal and total coliform bacteria content in the Banjir Kanal Barat river, and analyzing how to overcome the decline in the quality of waters of the Banjir Kanal Barat river due to faecal and total coliform bacteria pollution. The results showed that the water quality at the research location was still in the quality standard. The content of coliform dan faecal bacteria at the study site exceeds the quality standard, this is due to the influence of domestic waste from households. The thing that needs to be done is counseling the existence of a clean and healthy life, especially for people who are still throwing domestic waste into the river. The existence of water purification equipment is also very necessary to overcome this problem. Water quality management can be done with policy analysis. Regulations related to water quality management can be analyzed and then given solutions and recommendations related to these rules so that policies can be taken that are sustainable, integrated, and coordinated between various parties in managing river water quality and food security. Keywords: food security, water quality, river, faecal coliform, total coliform

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