The use of antibacterial activity of ZnO nanoparticles in the treatment of municipal wastewater

Nanotechnology holds great potential in advanced water and wastewater treatment to improve treatment efficiency. Zinc oxide nanoparticles (ZnO NPs) have received considerable attention due to their unique antibacterial activities toward various microorganisms that are commonly found in the environment. In the present study, ZnO NPs were synthesized through both mechano-chemical and sol–gel methods. The synthesized ZnO NPs were characterized through X-ray diffraction and transmission electron microscopy techniques. Then, their antibacterial activities against separated wastewater bacteria were evaluated by determining the zone inhibitor, the minimum inhibitory concentration, and the minimum bactericidal concentration. The results were compared with those obtained from wastewater after chlorine disinfection and ultraviolet (UV) disinfection. These studies demonstrated that the antibacterial activity of ZnO NPs depends on the type and the strain of bacteria. They have also demonstrated that the activity increases as the concentration of ZnO NPs increases. Overall, the experimental results suggest that ZnO NPs can potentially be an antibacterial reagent to treat wastewater. They can particularly be applied as a complementary method with UV disinfection. Thus, they can be developed as antibacterial agents to improve wastewater quality.

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Antibacterial Activity of Nickel -Doped ZnO\MWCNTs hybrid Prepared by Sol-Gel technique

The European Physical Journal Applied Physics ◽

10.1051/epjap/2021210115 ◽

2021 ◽

Author(s):

Selma M.H. AL-Jawad ◽

Zahraa S. Shakir ◽

Duha S. Ahmed

Keyword(s):

Antibacterial Activity ◽

Sol Gel ◽

Inhibition Zone ◽

Sem Analysis ◽

Morphological Properties ◽

X Ray Diffraction ◽

Zno Nps ◽

X Ray ◽

Vis Spectroscopy ◽

Doped Zno

ZnO/MWCNTs hybrid and doped with different concentration of Nickel element prepared by using Sol-gel been technique reported. All samples were prepared and characterized by X-Ray Diffraction Analysis (XRD), Energy Dispersive X-ray Spectroscopy (EDS), Fourier-Transform Infrared Spectroscopy (FTIR), Field-Emission Scanning Electron Microscopy (FE-SEM), and UV-Vis spectroscopy have been identified the structural, optical and morphological properties. X-ray diffraction showed the polycrystalline nature with hexagonal wutzite structure of hybrid and doped with Nickel. The crystalline size of the hybrid nanostructure was increasing from 23.73 nm to 34.59 nm. Besides, the UV-Vis spectroscopy showed a significant decrease in the band gap values from 2.97 eV to 2.01 eV. Whereas the FE-SEM analysis confirm the formation spherical shapes of ZnO NPs deposited on cylindrical tubes representing the MWCNTs. The antibacterial activity reveals that the inhibition zone of Ni doped-ZnO/MWCNTs hybrid was 28.5 mm, 26.5 mm toward E. coli and S. aureus bacteria, respectively.

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Antibacterial Activity of Ag-Doped TiO2 and Ag-Doped ZnO Nanoparticles

International Journal of Photoenergy ◽

10.1155/2018/5927485 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 19

Author(s):

Gebretinsae Yeabyo Nigussie ◽

Gebrekidan Mebrahtu Tesfamariam ◽

Berhanu Menasbo Tegegne ◽

Yemane Araya Weldemichel ◽

Tesfakiros Woldu Gebreab ◽

...

Keyword(s):

Antibacterial Activity ◽

Zno Nanoparticles ◽

Pathogenic Bacteria ◽

Anatase Phase ◽

Sol Gel ◽

X Ray Diffraction ◽

Zno Nps ◽

Sem Images ◽

X Ray ◽

Doped Zno

We report in this paper antibacterial activity of Ag-doped TiO2 and Ag-doped ZnO nanoparticles (NPs) under visible light irradiation synthesized by using a sol-gel method. Structural, morphological, and basic optical properties of these samples were investigated using X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectrum, and UV-Vis reflectance. Room temperature X-ray diffraction analysis revealed that Ag-doped TiO2 has both rutile and anatase phases, but TiO2 NPs only have the anatase phase. In both ZnO and Ag-doped ZnO NPs, the hexagonal wurtzite structure was observed. The morphologies of TiO2 and ZnO were influenced by doping with Ag, as shown from the SEM images. EDX confirms that the samples are composed of Zn, Ti, Ag, and O elements. UV-Vis reflectance results show decreased band gap energy of Ag-doped TiO2 and Ag-doped ZnO NPs in comparison to that of TiO2 and ZnO. Pathogenic bacteria, such as Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli, were used to assess the antibacterial activity of the synthesized materials. The reduction in the viability of all the three bacteria to zero using Ag-doped ZnO occurred at 60 μg/mL of culture, while Ag-doped TiO2 showed zero viability at 80 μg/mL. Doping of Ag on ZnO and TiO2 plays a vital role in the increased antibacterial activity performance.

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SYNTHESIS, CHARACTERIZATION AND ANTIBACTERIAL ACTIVITIES OF CHROMIUM OXIDE NANOPARTICLES AGAINST KLEBSIELLA PNEUMONIAE

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i2.15189 ◽

2017 ◽

Vol 10 (2) ◽

pp. 206 ◽

Cited By ~ 4

Author(s):

Poonam Sangwan ◽

Harish Kumar

Keyword(s):

Antibacterial Activity ◽

Klebsiella Pneumoniae ◽

Chromium Oxide ◽

Sol Gel ◽

Antibacterial Activities ◽

Penicillin G ◽

Oxide Nanoparticles ◽

Cr2o3 Nanoparticles ◽

Chromium Oxide Nanoparticles ◽

Gel Technique

Objective: This paper consists of synthesis of chromium oxide (Cr2O3) nanoparticles by sol-gel technique, their characterization and investigation of antibacterial activity of these nanoparticles against pathogenic bacteria by measuring zone of inhibition, colony forming units and optical density on solid agar media as well as in liquid medium.Method: The Cr2O3 nanoparticles were synthesized by sol-gel technique using TEOS as precursor. The synthesized chromium oxide nanoparticles were characterized by X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, UV-Visible spectroscopy and transmission electron microscopy (TEM) techniques. The antibacterial effect of these chromium oxide nanoparticles against Klebsiella pneumoniae was investigated both on the solid agar plates and in liquid medium supplemented with different concentrations of Cr2O3 nanoparticles. The antibacterial activity of chromium oxide nanoparticles was also compared with the antibacterial activities of the standard antibiotics such as ampicillin, chloramphenicol, penicillin G, streptomycin, sulphatriad and tetracycline which were taken in the form of hexa discs.Results: Average particle size of the Cr2O3 nanoparticles was found to be 24.0 nm. It was observed that Klebsiella pneumoniae is resistant to the penicillin G and ampicillin but Cr2O3 nanoparticles shows good antibacterial property. The minimum inhibitory concentration (MIC) of Cr2O3 for Klebsiella pneumoniae is 2.5 mg/ml. The bacterial growth was monitored by measuring zone of Inhibition, colony formation unit (CFU) and optical density (OD) method.Conclusion: Sol-gel technique is a convenient and easy technique for the synthesis of metal nanoparticles. Nanosized Cr2O3 particles showed an effective antibacterial activity against Klebsiella pneumoniae. Therefore, chromium oxide nanoparticles due to its low manufacturing cost and high effectiveness in antimicrobial properties may find wide applications in various industries to address safety issues.

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WO3-Doped TiO2Coating on Charcoal Activated with Increase Photocatalytic and Antibacterial Properties Synthesized by Microwave-Assisted Sol-Gel Method

Journal of Nanotechnology ◽

10.1155/2017/7902930 ◽

2017 ◽

Vol 2017 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Weerachai Sangchay

Keyword(s):

Methylene Blue ◽

Antibacterial Activity ◽

Water Purification ◽

Sol Gel ◽

Antibacterial Properties ◽

Antibacterial Activities ◽

Gel Method ◽

Sol Gel Method ◽

E Coli ◽

Microwave Assisted

WO3-doped TiO2coating on charcoal activated (CA) was prepared by microwave-assisted sol-gel method. The samples calcined at the temperature of 500°C for 2 h with a heating rate of 10°C/min were characterized by XRD, EDS, and SEM. The photocatalytic and antibacterial activities of WO3-doped TiO2coating on CA were investigated by means of degradation of a methylene blue (MB) solution and against the bacteriaE. coli, respectively. The effects of WO3concentration were discussed. The 1% WO3-doped TiO2coated CA seems to exhibit the higher photocatalytic and antibacterial activity than other samples. The WO3-doped TiO2coated on CA are expected to be applied as a photocatalyst for water purification.

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Synthesis and Antibacterial Activities of Ag/ZnO Nanoparticles

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.697.714 ◽

2016 ◽

Vol 697 ◽

pp. 714-717

Author(s):

Ya Wei Hu ◽

Hui Rong He ◽

Xia Kong ◽

Yang Min Ma

Keyword(s):

Antibacterial Activity ◽

Zno Nanoparticles ◽

Sol Gel ◽

Antibacterial Activities ◽

Diffusion Method ◽

Crystallographic Structure ◽

Zno Nanoparticle ◽

Disc Diffusion Method ◽

E Coli ◽

Zones Of Inhibition

Ag/ZnO nanoparticles with different Ag concentrations were fabricated through sol-gel method under 500 °C for 60 min with a heating rate 5 °C·min-1. The phase, the crystallographic structure and the surface topography of the Ag/ZnO nanoparticles were charactered by XRD and SEM. The antibacterial activities of the Ag/ZnO nanoparticles were performed by disc diffusion method. The results indicate that the as-prepared Ag/ZnO nanoparticles display great antibacterial activity than ZnO nanoparticle without Ag doping. And Ag/ZnO nanoparticles with 5 mol% Ag concentration show more excellent antibacterial activity toward S.aureus, B. subtilis, E. coli and P. aeruginosa with diameters zones of inhibition 21.7, 18.5, 18.4 and 17.8 mm than other Ag/ZnO nanoparticles. And the mechanism of ZnO antibacterial activity is discussed.

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Antioxidant and Antibacterial Activities of Artemisia herba-alba Asso Essential Oil from Middle Atlas, Morocco

Phytothérapie ◽

10.3166/phyto-2018-0057 ◽

2018 ◽

Vol 16 (S1) ◽

pp. S48-S54

Author(s):

Y. Ez zoubi ◽

S. Lairini ◽

A. Farah ◽

K. Taghzouti ◽

A. El Ouali Lalami

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Foodborne Pathogens ◽

Food Systems ◽

Antibacterial Activities ◽

Culture Collection ◽

Bornyl Acetate ◽

Bacterial Strains ◽

Disk Diffusion Assay ◽

Different Strains

The purpose of this study was to determine the chemical composition and to evaluate the antioxidant and antibacterial effects of the Moroccan Artemisia herba-alba Asso essential oil against foodborne pathogens. The essential oil of Artemisia herba-alba was analyzed by gas chromatography coupled with mass spectroscopy. The antibacterial activity was assessed against three bacterial strains isolated from foodstuff and three bacterial strains referenced by the ATCC (American Type Culture Collection) using the disk diffusion assay and the macrodilution method. The antioxidant activity was evaluated using the DPPH (2, 2-diphenyl-1- picrylhydrazyl) method. The fourteen compounds of the Artemisia herba-alba essential oil were identified; the main components were identified as β-thujone, chrysanthenone, α-terpineol, α-thujone, α-pinene, and bornyl acetate. The results of the antibacterial activity obtained showed a sensitivity of the different strains to Artemisia herba-alba essential oil with an inhibition diameter of 8.50 to 17.00 mm. Concerning the MICs (minimum inhibitory concentrations), the essential oil exhibited much higher antibacterial activity with MIC values of 2.5 μl/ml against Bacillus subtilis ATCC and Lactobacillus sp. The essential oil was found to be active by inhibiting free radicals with an IC50 (concentration of an inhibitor where the response is reduced by half) value of 2.9 μg/ml. These results indicate the possible use of the essential oil on food systems as an effective inhibitor of foodborne pathogens, as a natural antioxidant, and for potential pharmaceutical applications. However, further research is needed in order to determine the toxicity, antibacterial, and antioxidant effects in edible products.

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Nanofiltration of endocrine disrupting compounds

Water Science & Technology Water Supply ◽

10.2166/ws.2003.0184 ◽

2003 ◽

Vol 3 (5-6) ◽

pp. 321-327 ◽

Cited By ~ 2

Author(s):

M. Gallenkemper ◽

T. Wintgens ◽

T. Melin

Keyword(s):

Wastewater Treatment ◽

Municipal Wastewater ◽

Wastewater Treatment Plants ◽

Endocrine Disrupting Compounds ◽

Municipal Wastewater Treatment ◽

Water And Wastewater Treatment ◽

Wide Range ◽

Endocrine Disrupting ◽

Municipal Wastewater Treatment Plants ◽

Set Up

Endocrine disrupting compounds can affect the hormone system in organisms. A wide range of endocrine disrupters were found in sewage and effluents of municipal wastewater treatment plants. Toxicological evaluations indicate that conventional wastewater treatment plants are not able to remove these substances sufficiently before disposing effluent into the environment. Membrane technology, which is proving to be an effective barrier to these substances, is the subject of this research. Nanofiltration provides high quality permeates in water and wastewater treatment. Eleven different nanofiltration membranes were tested in the laboratory set-up. The observed retention for nonylphenol (NP) and bisphenol A (BPA) ranged between 70% and 100%. The contact angle is an indicator for the hydrophobicity of a membrane, whose influence on the permeability and retention of NP was evident. The retention of BPA was found to be inversely proportional to the membrane permeability.

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Microwave assisted synthesis of novel Pyrazole derivatives and their biological evaluation as anti-bacterial agents

Combinatorial Chemistry & High Throughput Screening ◽

10.2174/1386207323666201019152206 ◽

2020 ◽

Vol 23 ◽

Author(s):

Hadis Khodadad ◽

Farhad Hatamjafari ◽

Khalil Pourshamsian ◽

Babak Sadeghi

Keyword(s):

Antibacterial Activity ◽

Aromatic Aldehydes ◽

Catalytic Amount ◽

Antibacterial Activities ◽

Structural Features ◽

Biological Evaluation ◽

Aureus Strain ◽

Microwave Assisted Synthesis ◽

Pyrazole Derivatives ◽

Microwave Assisted

Aim and Objective: Microwave-assisted condensation of acetophenone 1 and aromatic aldehydes 2 gave chalcone analogs 3, which were cyclized to pyrazole derivatives 6a-f via the reaction with hydrazine hydrate and oxalic acid in the presence of the catalytic amount of acetic acid in ethanol. Materials and Methods: The structural features of the synthesized compounds were characterized by melting point, FT-IR, 1H, 13C NMR and elemental analysis. Results: The antibacterial activities of the synthesized pyrazoles was evaluated against three gram-positive bacteria such as Enterococcus durans, Staphylococcus aureus, Bacillus subtilis and two gram-negative bacteria such as Escherichia coli and Salmonella typhimurium. Conclusion: All the synthesized pyrazoles showed relatively high antibacterial activity against S. aureus strain and none of them demonstrated antibacterial activity against E. coli.

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In Vitro Anti-Biofilm and Antibacterial Properties of Streptococcus downii sp. nov.

Microorganisms ◽

10.3390/microorganisms9020450 ◽

2021 ◽

Vol 9 (2) ◽

pp. 450

Author(s):

Maigualida Cuenca ◽

María Carmen Sánchez ◽

Pedro Diz ◽

Lucía Martínez-Lamas ◽

Maximiliano Álvarez ◽

...

Keyword(s):

Antibacterial Activity ◽

Quantitative Polymerase Chain Reaction ◽

Bacterial Load ◽

Antibacterial Properties ◽

Antibacterial Activities ◽

Oral Bacteria ◽

Periodontal Pathogens ◽

Biofilm Model ◽

Biofilm Development

The aim of this study was to evaluate the potential anti-biofilm and antibacterial activities of Streptococcus downii sp. nov. To test anti-biofilm properties, Streptococcus mutans, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans were grown in a biofilm model in the presence or not of S. downii sp. nov. for up to 120 h. For the potential antibacterial activity, 24 h-biofilms were exposed to S. downii sp. nov for 24 and 48 h. Biofilms structures and bacterial viability were studied by microscopy, and the effect in bacterial load by quantitative polymerase chain reaction. A generalized linear model was constructed, and results were considered as statistically significant at p < 0.05. The presence of S. downii sp. nov. during biofilm development did not affect the structure of the community, but an anti-biofilm effect against S. mutans was observed (p < 0.001, after 96 and 120 h). For antibacterial activity, after 24 h of exposure to S. downii sp. nov., counts of S. mutans (p = 0.019) and A. actinomycetemcomitans (p = 0.020) were significantly reduced in well-structured biofilms. Although moderate, anti-biofilm and antibacterial activities of S. downii sp. nov. against oral bacteria, including some periodontal pathogens, were demonstrated in an in vitro biofilm model.

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