Modulation of the Lipid Profile, Hepatic and Renal Antioxidant Activities, and Markers of Hepatic and Renal Dysfunctions in Alloxan-Induced Diabetic Rats by Virgin Coconut Oil

2019 ◽  
Vol 19 (7) ◽  
pp. 1032-1040
Author(s):  
Chinedum Eleazu ◽  
Chima E. Ekeleme ◽  
Ademola Famurewa ◽  
Mahaneem Mohamed ◽  
Gabriel Akunna ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Coconut Oil ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Distilled Water ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Virgin Coconut Oil

Background:Research studies that holistically investigated the effect of administration of Virgin Coconut Oil (VCO) on diabetic humans or animals are limited in literature.Objective:To investigate the effect of administration of VCO on lipid profile, markers of hepatic and renal dysfunction, and hepatic and renal antioxidant activities of alloxan induced diabetic rats.Methods:Twenty-four male albino rats were used, and they were divided into four groups of six rats each. Group 1 (Normal Control, NC) received distilled water (1 mL/kg); Group 2 (VCO Control) received VCO (5 mL/kg); Group 3 (Diabetic Control, DC) received distilled water (1 mL/kg); Group 4 (Test Group, TG) received 5 ml/kg of VCO.Results:There were no significant differences in blood glucose, body weights, relative liver weights, relative kidney weights, hepatic and renal Superoxide Dismutase (SOD) activities, Malondialdehyde (MDA), albumin, aspartate Amino Transaminase (AST), alanine Amino Transaminase (ALT), Alkaline Phosphatase (ALP), urea, creatinine, uric acid, total cholesterol, triacylglycerol, Very Low Density Lipoprotein cholesterol (VLDL) and Low Density Lipoprotein cholesterol (LDL) concentrations; significant increases in renal Glutathione (GSH), hepatic catalase, Glutathione Peroxidase (GPx) and GSH but significant reduction in renal GPx and catalase activities of VCO control group compared with NC group. There were significant increases in blood glucose, relative liver and kidney weights, hepatic GPx, hepatic and renal MDA concentration, ALP, AST, ALT, urea, creatinine, uric acid, triacylglycerol, total cholesterol, LDL and VLDL concentrations; and significant decreases in body weight, hepatic SOD and GSH activities and albumin concentration but no significant difference in hepatic catalase activity of DC group compared with NC group. Administration of VCO to diabetic rats positively modulated these parameters compared with the diabetic control.Conclusion:The study showed the potentials of VCO in the management of hyperlipidemia, renal and hepatic dysfunctions imposed by hyperglycemia and by oxidative stress in diabetic rats.

scholarly journals Evaluation of Antihyperlipidaemic and Antioxidant Activity of Astercantha longifolia (Linn.) Nees and Pergularia daemia (Forsskal) Chiov

2020 ◽  
pp. 134-138
Author(s):  
T. P. Kumari Pushpa Rani ◽  
A. Doss ◽  
S. P. Anand
Keyword(s):  
Antioxidant Activity ◽  
Biochemical Parameters ◽  
Methanol Extract ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol

Objective: To evaluate the antioxidant and antihyperlipidaemic effect of methanol extracts of Asteracantha longifolia and Pergularia daemia leaf in alloxan induced diabetic rats. Methods: Swiss albino rats were made diabetic by a single dose of alloxan monohydrate (150 mg/kg i.p.). Blood glucose levels and body weights of rats were measured using on weekly intervals i.e day 0,7,14 and 21 after daily administration of both extracts at dose 200 mg/kg bw. Other biochemical parameters such as serum cholesterol, triglycerides, low density lipoprotein-cholesterol, very low density lipoprotein-cholesterol, high density lipoprotein-cholesterol, phospholipids and serum protein, albumin, globulin levels were also measured at the end of study. The antioxidant enzymes (CAT, SOD & GSH) were also measured in the diabetic rats. Results: In the acute toxicity study, methanol extract of both plants were non-toxic at 2000 mg/kg in rats. The increased body weight, decreased blood glucose and other biochemical parameters level were observed in diabetic rats treated with methanol extracts of both plants compared to diabetic control rats. The antioxidant activity of both plant extracts were also exhibited significant activity. In diabetic rats, methanol extract of both plants administration, altered lipid profiles were reversed to near normal than diabetic control rats. Conclusions: Methanol extract of both plants (Asteracantha longifolia and Pergularia daemia) leaf possesses significant antioxidant and antihyperlipidaemic activity in diabetic rats.

scholarly journals THE EFFECT OF HYDROLYZED VIRGIN COCONUT OIL ON LIPID PROFILE AND LIVER ENZYMES IN DYSLIPIDEMIC RATS

2018 ◽  
Vol 11 (10) ◽  
pp. 406
Author(s):  
Linda Margata ◽  
Jansen Silalahi ◽  
Urip Harahap ◽  
Denny Satria
Keyword(s):  
Lipid Profile ◽  
Liver Enzymes ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Coconut Oil ◽  
Lipoprotein Cholesterol ◽  
Atherogenic Index ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Virgin Coconut Oil

Objective: The objective of this study was to investigate the effect of virgin coconut oil (VCO) and hydrolyzed VCO (HVCO) on lipid profile and liver enzymes in dyslipidemic rats.Methods: VCO was hydrolyzed using lipase from Rhizomucor miehei (active on sn-1,3 position). Thirty male rats (150–200 g) were induced with 2 ml/kg body weight (BW) egg yolk and lard oil twice a day for 30 days. Rats were divided into six groups which were given with sodium carboxymethylcellulose 0.5%, atorvastatin, VCO (4 and 6 ml/kg BW), and HVCO (4 and 6 ml/kg BW). Lipid profile including total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), very low-density lipoprotein cholesterol (VLDL-C), and liver enzymes including serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) were measured after 14 days of treatment.Results: The results of this study show that VCO and HVCO improve lipid profile (decrease in TC, TG, LDL-C, and VLDL-C, but increase in HDL-C). VCO and HVCO also lower atherogenic index, TC/HDL-C ratio, LDL-C/HDL-C ratio, SGOT, and SGPT. Result shows that HVCO improves lipid profile and liver enzymes better than VCO does.Conclusion: VCO and HVCO improve lipid profile in dyslipidemic rats, not atherogenic, and not toxic to the liver. HVCO causes better lipid profile improvement, especially with 6 ml/kg BW dosage.

scholarly journals Dietary Consumption of Virgin Coconut Oil Ameliorates Lipid Profiles in Diabetic Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
A. M. Akinnuga ◽  
S. O. Jeje ◽  
O. Bamidele ◽  
V. E. Sunday
Keyword(s):  
Diabetes Mellitus ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Coconut Oil ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Virgin Coconut Oil ◽  
Group Iii ◽  
Group I ◽  
Group Ii

Virgin coconut oil (VCO) is a saturated fat with promising antidiabetic properties but its ameliorative effect on lipid profiles in diabetics is rarely reported. Therefore, in this study, a total of fifteen (15) male rats weighing 200–250 g were divided into 3 experimental groups (n=5). Group I (control) and Group II (diabetic control group) were fed a normal rat chow while Group III (diabetic test group) was fed a 10% VCO diet for 3 weeks. Group II and Group III were made diabetic by intraperitoneal injection of 150 mg/kg of alloxan. After 72 hours of injection, blood glucose was tested to confirm diabetes mellitus. After 3 weeks, the animals were sacrificed to collect blood samples for lipid profile analysis. The results showed a significant increase in concentrations of triglyceride, total cholesterol, low density lipoprotein, and very low density lipoprotein and decrease in concentration of high density lipoprotein in Group II when compared to Group I. Also, the concentrations of triglyceride, total cholesterol, low density lipoprotein, and very low density lipoprotein except high density lipoprotein significantly reduced in Group III when compared to Group II (P<0.01, 0.001). VCO consumption can be claimed to ameliorate lipid levels in diabetes mellitus.

scholarly journals Blood Lipids and Its Atherogenic Indices in Alloxan Induced Diabetic Male Rats

2020 ◽  
pp. 25-33
Author(s):  
O. D. Chikezie ◽  
S. C. Meludu ◽  
I. S. I. Ogbu ◽  
B. N. Egejuru ◽  
T. Ude ◽  
...  
Keyword(s):  
Blood Lipids ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Lipoprotein Cholesterol ◽  
Male Rats ◽  
Low Density Lipoprotein Cholesterol ◽  
Atherogenic Indices ◽  
Diabetes Induction

Background: Diabetes mellitus is a group of metabolic disorders which result to excessive accumulation of blood sugar over a prolonged period. Due to higher risk of diabetes mellitus to cardiovascular disease, it is crucial to identify and address these cardiovascular risks. This study assessed the effects of diabetes on levels of some blood lipids and its atherogenic indices in diabetic male rats. Methods: This is an experimental study that involved 40 apparently healthy adult male albino rats (wistar strain) which were randomly assigned to five groups (A, B, C, D and E) of eight (8) animals each. Group A (Normal Control of No intervention for 72 hours), Group B (Diabetic rats of 72 hours post diabetes induction), Group C (metformin treated diabetic rats), Group D (Diabetic Control untreated) and Group E (Normal Control of 3 weeks post diabetes induction). Seven milliliters of fasting blood sample were collected from all the subjects. Serum levels of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-c), low density lipoprotein cholesterol (LDL-c) and very low density lipoprotein cholesterol (VLDL-c) were determined using standard methods. Atherogenic indices, non HDL cholesterol (Non HDL-c), cardio risk ratio (CRR), atherogenic index of plasma (AIP), atherogenic coefficient (AC) and atherosclerosis index (AI) were calculated. It was analyzed statistically using SPSS version 23.0. Results: The mean values of HDL-c was significantly higher in the treated diabetic group when compared with untreated diabetic control (P<0.05) while TC, TG, LDL-c, VLDL-c, Non HDL-c, CRR, AIP, AC and AI were significantly lower in treated diabetics when compared to the untreated diabetic control (P<0.05). Also, blood mean levels of HDL-c were significantly lower in the diabetic groups (treated and untreated) when compared with non diabetic control (P<0.05) while TC, TG, LDL-c, VLDL-c, Non HDL-c, CRR, AIP, AC and AI were significantly higher in the diabetic groups (treated and untreated) when compared with non diabetic control (P<0.05). Conclusion: The study suggests that atherogenic indices can serve as predictive pointer for future cardiovascular event especially, when LDLc value is normal. Also hyperglycemia could cause significant alterations of lipids, but metformin treatment has showed not only hypoglycemic effect, but also anti-hyperlipidemic properties.

scholarly journals Effect of Antioxidant Mineral Elements Supplementation in the Treatment of Hypertension in Albino Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
S. A. Muhammad ◽  
L. S. Bilbis ◽  
Y. Saidu ◽  
Y. Adamu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activities ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Treatment Of Hypertension ◽  
Total Antioxidant

Oxidative stress has been implicated in various pathologies, including hypertension, atherosclerosis, diabetes, and chronic renal disease. The current work was designed with the aim of investigating the potentials of antioxidants copper, manganese, and zinc in the treatment of hypertension in Wistar rats. The rats were fed 8% NaCl diet for 5 weeks and treatment with supplements in the presence of the challenging agent for additional 4 weeks. The supplementation significantly decreased the blood pressure as compared with hypertensive control. The result also indicated significant decreased in the levels of total cholesterol, triglyceride, low-density lipoprotein cholesterol and very low-density lipoprotein cholesterol, malondialdehyde, insulin and increase in the high-density lipoprotein cholesterol, total antioxidant activities, and nitric oxide of the supplemented groups relative to the hypertensive control. The average percentage protection against atherogenesis indicated 47.13 ± 9.60% for all the supplemented groups. The mean arterial blood pressure showed significant positive correlation with glucose, total cholesterol, triglyceride, low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, atherogenic index, insulin resistance and malondialdehyde while high density lipoprotein-cholesterol and total antioxidant activities showed negative correlation. The result therefore indicated strong relationship between oxidative stress and hypertension and underscores the role of antioxidant minerals in reducing oxidative stress, dyslipidemia, and insulin resistance associated with hypertension.

scholarly journals Protein Isolate from Parkia biglobosa Seeds Improves Dyslipidaemia and Cardiac Oxidative Stress in Streptozotocin-Induced Diabetic Rats

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 481
Author(s):  
Bolajoko Idiat Ogunyinka ◽  
Babatunji Emmanuel Oyinloye ◽  
Foluso Oluwagbemiga Osunsanmi ◽  
Unathi Kolanisi ◽  
Andrew Rowland Opoku ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Protein Isolate ◽  
Diabetic Rats ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Parkia Biglobosa ◽  
Low Density Lipoprotein Cholesterol ◽  
Biomarkers Of Oxidative Stress

Reports from previous studies now provide evidence that dyslipidaemia and oxidative stress play crucial roles in the pathogenesis and progression of diabetes and its related complications. This research is aimed to investigate the potential effects of protein isolate from Parkia biglobosa seeds (PBPI) in streptozotocin (STZ)-induced diabetic rats by measuring blood glucose levels, changes in lipid metabolism and biomarkers of oxidative stress. Diabetic rats were treated orally with graded doses of PBPI, 200 mg/kg bw and 400 mg/kg bw, and 5 U/kg, intraperitoneal (i.p.) of insulin once daily for 28 days with the fasting blood glucose (FBG) monitored weekly. The effect of PBPI on the serum lipid profile was measured while the extent of lipid peroxidation (LPO), as well as antioxidant parameters (superoxide dismutase; SOD, catalase; CAT, glutathione-S-transferase; GST and total glutathione; total GSH), was determined in the cardiac homogenates of diabetic rats. At the tested doses, treatment with PBPI was significantly effective in lowering FBG, serum triglyceride, cholesterol, low-density lipoprotein cholesterol (LDL-c) and very low-density lipoprotein cholesterol (VLDL-c), while concurrently increasing high-density lipoprotein cholesterol (HDL-c). PBPI also significantly decreased the elevations witnessed in LPO levels and restored the biomarkers of oxidative stress in the cardiac homogenate of experimental rats. The results from this study demonstrate that PBPI could improve dyslipidaemia and cardiac oxidative stress in the experimental diabetic animal model possibly by reducing and effectively scavenging reactive oxygen species (ROS) production as well as by increasing antioxidant capacity in combating oxidative stress. Therefore, it can be concluded that PBPI could be explored in the development of a potent cardioprotective supplement or adjuvant therapy towards the management of diabetes and its related complications.

scholarly journals Antioxidant potential and low-density lipoprotein cholesterol (LDL-c) uptake of the black seed and honey mixture on human hepatocellular carcinoma (HepG2) cells

Food Research ◽  
2021 ◽  
Vol 5 (6) ◽  
pp. 141-149
Author(s):  
N.S. Mohd Isa ◽  
J.S. Ng ◽  
F. Tufail Ahmad ◽  
M.N.I. Kassim ◽  
Norhayati H. ◽  
...  
Keyword(s):  
Hepg2 Cells ◽  
Antioxidant Activities ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Lipid Lowering ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Black Seed ◽  
Significant Difference

High lipid levels especially low-density-lipoprotein cholesterol (LDL-c) are associated with increased risk of cardiovascular (CVD) and coronary heart disease (CHD). Both black seed (Nigella sativa L.) and honey are well-known in the hypolipidemic potential and have CVD protective effects. In the present study, LDL-c uptake of the black seed and honey mixture was tested on HepG2 cells. Antioxidant activities of black seed and honey mixtures were determined through the 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) assay. The anticancer potential of black seed and honey mixtures in HepG2 cells was performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay. Black seed possessed the highest antioxidant activities with EC50 6.54 mg/mL as compared to honey with EC50 value 9.56 mg/mL while the black seed and honey mixture have EC50 between black seed and honey. From the results obtained, no synergistic effect was observed in the mixtures as the EC50 values were within the range of black seed and honey. Furthermore, no significant difference (p>0.05) among ratios (1:1, 2:1 and 1:2). However, the decrease in cell proliferation was the highest in black seed and honey mixture at 1:1 ratio (p<0.05) than individually treated black seed and honey. Thus, the black seed and honey mixture at ratio 1:1 was the most potent anticancer agent with an IC50 value of 7.44 μg/mL. The present study illustrated that black seed and honey mixtures possess a lipid-lowering effect via LDL-c uptake in HepG2 cells (p<0.05). The highest LDL-c uptake was observed at 15 μg/mL with the treatment of black seed and honey mixture at 1:2 ratio which was 294.4%. Further studies should be conducted on primary human liver cells to further justify the correlation between the antioxidant level and LDLc uptake mechanism of black seed and honey mixtures.

In vivo Evaluation of Anti-Hyperglycemic, Anti-hyperlipidemic and Anti-Oxidant Status of Liver and Kidney of Thymol in STZ-Induced Diabetic Rats

Drug Research ◽  
2018 ◽  
Vol 69 (01) ◽  
pp. 46-52 ◽  
Author(s):  
Bijan Oskouei ◽  
Soheil Abbaspour-Ravasjani ◽  
Seyed Jamal Musavinejad ◽  
Seyed Ahmad Salehzadeh ◽  
Alireza Abdolhosseinzadeh ◽  
...  
Keyword(s):  
Liver Function ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Diabetic Rats ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Positive Effects ◽  
Liver And Kidney ◽  
Anti Oxidant

Abstract Aim The evaluation of the positive and protective effects thymol as a natural compound on the STZ -induced diabetic rats. Materials and methods In this study, seven groups of the streptozotocin induced diabetic and normal rats (overall 42 males) were tested through various biochemical and histopathological factors such as: serum glucose, insulin, creatinine, lipids, lipoproteins, liver function-related enzymes, and antioxidant status in liver and kidney. Results The obtained results in this work indicated that thymol had a significant anti-hypoglycemic, anti-hypolipidemic activities on the STZ-induced diabetic rats. Further, the assessment different biochemical parameters revealed that the levels of creatinine, low-density lipoprotein cholesterol, very low density lipoprotein cholesterol, and liver function-related enzymes such as aspartate aminotransferase and alanine aminotransferase were decreased in the treated diabetes rats under 20 and 40 mg/kg thymol compared to the control diabetes group. Considerably, the anti-oxidant enzymes status that were achieved from the liver and kidney organs were modulated after treatment with thymol in the diabetic rats. Conclusion The results of this research has brough a new aspect concerning the proteevtive and positive effects of thymol on the diabetic complications in the animal model.

Sign in / Sign up

Export Citation Format

Share Document