Hepatoprotective Activity of Helicteres isora Ethanol Extract Against Paracetamol-Induced Liver Injury in Mice

Helicteres isora L. is a medicinal plant which is used in several diseases, such as snake-bite, dog-bite, diarrhoea and constipation in a new born baby, gastrointestinal disorders, diabetes, cancer, and infections. This plant has also been used in the management of liver damage through traditional medicine. However, the hepatoprotective activity of H. isora L. ethanolic extract has not been reported so much. The present work was carried to investigate the hepatoprotective effect of H. isora L. against paracetamol-induced liver injury in Swiss mice. Paracetamol (PCM) is widely used as an analgesic and antipyretic drug which at high dose can lead to undesirable side effects, such as hepatotoxicity. Paracetamol induce hepatotoxicity was evaluated by an increase (P<0.05) in AST and ALT serum activity. Paracetamol hepatotoxicity was also manifested by an increase in (P<0.05) lipid peroxidation and depletion of reduced glutathione (GSH) in liver tissue. Ethanol extract of H. isora L. (250, 500 and 1000 mg/kg bw/day) significantly restored the PCM-induced alterations in the biochemical activities of blood and liver tissues. The hepatoprotective effect of H. isora L. was also confirmed by the histopathological examination of liver tissue. Histopathological examination of liver sections in mice administered with 1000 mg/kg bw/day doses of the extract were perfectly protected almost similar to those of untreated mice. The results indicated the hepatoprotective nature of studied plants extract against paracetamol induced toxicity. Our study scientifically validates the folkloric claim as well as traditional uses of H. isora L. as hepatoactive medicine. The results of this study suggests a new direction in the treatment of liver disease in future.

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EVALUATION OF HEPATOPROTECTIVE EFFECT OF ETHANOLIC EXTRACT OF ROOT OF PUNICA GRANATUM IN RATS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i7.18172 ◽

2017 ◽

Vol 10 (7) ◽

pp. 159

Author(s):

Bushra Hasan Khan ◽

Farida Ahmad ◽

Jameel Ahmad ◽

Syed Mobashir Yunus

Keyword(s):

Liver Injury ◽

Histopathological Examination ◽

Ethanolic Extract ◽

Punica Granatum ◽

Treated Group ◽

Hepatoprotective Activity ◽

Hepatoprotective Effect ◽

Total Serum ◽

Physical Parameters ◽

Standard Drug

Objective: To evaluate the hepatoprotective effect of ethanolic extract of the root (REE) of Punica granatum.Methods: This study was conducted on adult albino Wistar rats of either sex weighing 150-200 g. Animals were divided into five groups (n=5). Liver injury was produced by carbon tetrachloride (CCl4) 1 ml/kg dissolved in olive oil (1:1) given intraperitoneally on day 1 and day 4 of the study duration of 14 days. Silymarin (50 mg/kg/d) orally was used as standard drug. Test groups received an REE of P. granatum (REE) at doses of 200 and 400 mg/kg/day orally along with CCl4. On the 15th day, all animals were sacrificed, and blood was collected. Liver was sent for histopathological examination. The hepatoprotective effect of REE was evaluated by assessment of physical parameters, histopathological examination and biochemical parameters such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and total serum bilirubin.Results: The administration of REE of P. granatum at doses of 200 and 400 mg/kg/day orally, exhibited a highly significant decrease in the rise of mean serum AST, ALT, ALP, and total bilirubin as compared to CCl4 treated group (p<0.001). Histopathological examination of the liver also suggested hepatoprotective effect of REE of P. granatum by restoration of hepatic architecture toward normal. Decrease in the extent of centrilobular necrosis was observed in REE (200 and 400 mg/kg/day) treated rats when compared to CCl4 treated group.Conclusion: This study demonstrated hepatoprotective activity of REE of P. granatum against CCl4 induced liver injury in rats.

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Hepatoprotective Activity of Curcuma mangga Extract on Paracetamol-Induced Male Mice

Indonesian Journal of Pharmaceutical and Clinical Research ◽

10.32734/idjpcr.v1i2.432 ◽

2018 ◽

Vol 1 (2) ◽

pp. 35-40

Author(s):

Yuandani ◽

Silvia Mardaliza ◽

Marianne

Keyword(s):

Ethanol Extract ◽

Serum Levels ◽

Hepatic Necrosis ◽

Hepatoprotective Activity ◽

Negative Control ◽

Hepatoprotective Effect ◽

Hepatic Tissue ◽

High Dose ◽

Male Mice ◽

Mice Liver

This study was carried out to investigate the protective effect of ethanol extract of Curcuma mangga rhizomes on paracetamol-induced hepatotoxicity. High dose of paracetamol (1.35g/kg bw) was used to induce hepatic necrosis of mice liver. The male mice received ethanol extract of C. mangga rhizomes (100, 200 and 400 mg/kg BW) for 7 days. The hepatoprotective actvity of extract was compared to normal, positive (curcuma) and negative control. The liver function was evaluated by measuring the biochemistry parameters which include alanine aminotransferase (ALT) and aspartate aminotransferase (AST). In addition, histophatological study on hepatic tissue section was also carried out. The C. mangga extract displayed hepatoprotective effect except at dose of 100 mg/kg bw. The increasing of serum levels of AST and ALT were inhibited after treatment with ethanol extract at doses of 200 and 400 mg/kb bw which was comparable with normal and curcuma as postive control (p>0.05). In addition, histological assessment of hepatic tissue demonstrated no liver damage, specially at dose of 400 mg/kb BW. The result indicate that ethanol extract of C. mangga rhizomes has hepatoprotective effect, especially at doses of 200 and 400 mg/kg bw . Keywords: C. mangga, rhizomes, biochemistry parameters, histopathology

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Hepatoprotective Effect ofShidagonglaoon Acute Liver Injury Induced by Carbon Tetrachloride

The American Journal of Chinese Medicine ◽

10.1142/s0192415x0900751x ◽

2009 ◽

Vol 37 (06) ◽

pp. 1085-1097 ◽

Cited By ~ 9

Author(s):

Jung Chao ◽

Meng-Shiou Lee ◽

Sakae Amagaya ◽

Jiunn-Wang Liao ◽

Jin-Bin Wu ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Liver Injury ◽

Acute Liver Injury ◽

Ethanol Extract ◽

Neutrophil Infiltration ◽

Treated Group ◽

Hepatoprotective Activity ◽

Hepatoprotective Effect ◽

Oxidative Enzymes ◽

Tnf Α

This study investigates the hepatoprotective activity of ethanol extract from Shidagonglao roots (SDGLEtOH). The hepatoprotective effect of SDGLEtOH(20, 100 and 500 mg/kg) was analyzed on carbon tetrachloride ( CCl4)-induced acute liver injury. Rats pretreated orally with SDGLEtOH(100 and 500 mg/kg) and silymarin (200 mg/kg) for 3 consecutive days prior to the administration of a single dose of 50% CCl4(0.10 ml/100 g of bw, ip) significantly prevented the increases in the activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in CCl4-treated rats. Histological analysis also showed that SDGLEtOH(100 and 500 mg/kg) and silymarin reduced the incidence of liver lesions including vacuole formation, neutrophil infiltration and necrosis of hepatocytes induced by CCl4in rats. Moreover, the SDGLEtOH(100 and 500 mg/kg) increased the activities of anti-oxidative enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GRd) and decreased malondialdehyde (MDA) level in liver, as compared to those in the CCl4-treated group. Furthermore, SDGLEtOH(100 and 500 mg/kg) and silymarin attenuated the increased levels of tumor necrosis factor-α (TNF-α) in serum and nitric oxide ( NO ) in liver as compared to the CCl4-treated group. The hepatoprotective mechanisms of SDGLEtOHare likely related to inhibition of TNF-α, MDA and NO productions via increasing the activities of antioxidant enzymes (SOD, GPx and GRd). These experimental results suggest that SDGLEtOHcan attenuate CCl4-induced acute liver injury in rats.

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Hepatoprotective effect of ethanolic extract of sugarcane (Saccharum officinarum Linn.) leaves

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2020-0432 ◽

2021 ◽

Vol 32 (4) ◽

pp. 533-540

Author(s):

Ika P. Dewi ◽

Rifdah B. Kwintana ◽

Jihan U. Ulinnuha ◽

Fadhillah Rachman ◽

Fransiska M. Christianty ◽

...

Keyword(s):

Ethanolic Extract ◽

Ethanol Extract ◽

Saccharum Officinarum ◽

Hepatoprotective Activity ◽

Negative Control ◽

Hepatoprotective Effect ◽

Acute Injury ◽

Wistar Strain ◽

Potential Activity ◽

Anti Inflammation

Abstract Objectives The sugarcane leaf is rich inphytochemical content. It is rarely used because it is a waste although it has potential activity as antimutation, anti inflammation, and antioxidation. There is no study about its hepatoprotective activity yet. This study was conducted to determine the hepatoprotection of sugarcane leaves in tested animals with liver acute injury induced by carbon tetrachloride (CCl4). Methods Twenty-four Wistar strain rats were divided into three groups of experimental animals (dose 300, 400, and 500 mg/kg) and three control groups (normal, positive, and negative). The ethanol extract of sugarcane leaves obtained from Panti, Jember, was made using the maceration method. The animals were treated for 14 days by giving the extract to the treatment group. One hour after treatment on the last day, the test animals were given CCl4 intraperitoneally except for the normal group. On the 15th day, the blood of the test animal was taken to be tested for the biochemical value of the liver (aspartate transaminase (AST), alanine aminotransferase (ALT), alanine phosphatase (ALP), and bilirubin) and examined for its liver to be made histological preparations. Results The results showed that the treatment with a dose of 500 mg/kg was able to decrease AST, ALT, ALP, and bilirubin parameters compared to the negative control. The extract also provided improvements in liver tissue histology compared to the negative control. Conclusions Sugarcane leaf ethanol extract (SCLE) has a potential hepatoprotective effect.

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Hepatoprotective Effect of Citrus Sinensis Peel Extract Against Isoniazid and Rifampicin-induced Liver Injury in Wistar Rats

Majalah Obat Tradisional ◽

10.22146/mot.45762 ◽

2019 ◽

Vol 24 (3) ◽

pp. 197

Author(s):

Edward Kosasih ◽

Linda Chiuman ◽

I Nyoman Ehrich Lister ◽

Edy Fachrial

Keyword(s):

Liver Injury ◽

Citrus Sinensis ◽

Wistar Rats ◽

Histopathological Examination ◽

Ethanolic Extract ◽

Hepatoprotective Effect ◽

Control Groups ◽

Male Wistar Rats ◽

The Rich ◽

Peel Extract

Tuberculosis (TB) is one of the leading causes of death in developing countries. One of the problems in controlling TB disease is that most anti-tuberculosis drugs are hepatotoxic. Citrus sinensis peel extract is the rich source of secondary metabolites with high potential effectiveness as an antioxidant. In the present study, we evaluate the hepatoprotective effect of Citrus sinensis peel ethanolic extract (CSPEE) on isoniazid and rifampicin-induced liver injury in Wistar rats. Twenty five adult male Wistar rats were divided into 5 groups of 5 each : control, (INH+RIF) (50 mg/kg bw once a day for 14 days), (INH+RIF) + various dose of CSPEE (300, 450, 600 mg/kg bw). CSPEE was given orally once a day for 14 days followed by administration of INH + RIF suspension. The measurement of serum ALT and AST were carried out on the 15th day. Histopathologic examination of the liver was also performed. The Serum ALT and AST of the rats that induced with INH + RIF were increased significantly (P<0.001) compare to those of control groups, and the histopathologic slides showed steatosis, vacuolation and necrosis of hepatic cells. The serum ALT and AST in groups treated with CSPEE were not significantly different (p>0.05) with those of control groups. The serum ALT and AST and histopathological examination of the liver of the group that administered 600 mg/kg CSPEE were closest to normal rats. Citrus sinensis peel extract exhibits hepatoprotective effect on liver injury induced with INH + RIF in Wistar rats.

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Hepatoprotective Role of Ethanolic Extract ofVitex negundoin Thioacetamide-Induced Liver Fibrosis in Male Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2013/739850 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 20

Author(s):

Farkaad A. Kadir ◽

Normadiah M. Kassim ◽

Mahmood A. Abdulla ◽

Wageeh A. Yehye

Keyword(s):

Body Weight ◽

Liver Fibrosis ◽

Histopathological Examination ◽

Weight Ratio ◽

Ethanolic Extract ◽

Hepatoprotective Activity ◽

Male Rats ◽

High Dose ◽

Sprague Dawley ◽

Standard Drug

The hepatoprotective activity of ethanolic extract from the leaves ofVitex negundo(VN) was conducted against thioacetamide- (TAA-) induced hepatic injury inSprague Dawleyrats. The therapeutic effect of the extract was investigated on adult male rats. Rats were divided into seven groups: control, TAA, Silymarin (SY), and VN high dose and low dose groups. Rats were administered with VN extract at two different doses, 100 mg/kg and 300 mg/kg body weight. After 12 weeks, the rats administered with VN showed a significantly lower liver to body weight ratio. Their abnormal levels of biochemical parameters and liver malondialdehyde were restored closer to the normal levels and were comparable to the levels in animals treated with the standard drug, SY. Gross necropsy and histopathological examination further confirmed the results. Progression of liver fibrosis induced by TAA in rats was intervened by VN extract administration, and these effects were similar to those administered with SY. This is the first report on hepatoprotective effect of VN against TAA-induced liver fibrosis.

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HEPATOPROTECTIVE ACTIVITY FROM ETHANOL EXTRACT OF PUGUN TANO’S LEAVE (Curanga fel-terrae (Lour.) Merr.)

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i11.19127 ◽

2017 ◽

Vol 10 (11) ◽

pp. 78

Author(s):

Urip Harahap ◽

Marianne Bastian ◽

Sri Yuliasmi ◽

Dadang Irfan Husori ◽

Popi Patilaya ◽

...

Keyword(s):

Ethanolic Extract ◽

Ethanol Extract ◽

Wistar Rat ◽

Negative Control Group ◽

Hepatoprotective Activity ◽

Positive Control ◽

Negative Control ◽

Normal Group ◽

Control Group ◽

High Dose

Objective: The aim of this study is to observe the activity of ethanol extract of Curanga fel-terrae leave in preventing the damaged of liver which is induced by high dose of paracetamol. Methods: This research was conducted by using Wistar rat divided into 6 groups. Group 1 was the normal group. Group 2, 3, 4, 5 and 6 received CMC-Na 0.5% (negative control), C. fel-terrae ethanolic extract (CFEE) at the doses of 125, 250 and 500 mg/kg, catechin 2 mg/kg (positive control),respectively during 7 days continued and followed by given paracetamol dose of 2.5g/kg 8 hours after that. Hepatoprotective activity was carried out toward parameter of AST, ALT as well as histopathology of the liver. Results: The results showed that high-dose paracetamol dose of 2.5g/kg bw can cause liver damaged which can be seen by the increasing of the level of AST and ALT compared to the normal group (p<0.05). The usage of three doses of CFEEfor 7 days showed the prevention of the increasing of the level of AST and ALT compared to negative control group (p<0.05). Furthermore, the histopathology study revealed that the three doses of extract could protect the liver. Conclusion: The C. fel-terrae ethanolic extract (CFEE) at the doses of 125, 250 and 500 mg/kg bw which was given for 7 days can prevent the liver from the damage caused by high-dose of paracetamol. Keyword: Curanga fel-terrae (Lour.) Merr., paracetamol, liver, hepatoprotective

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HEPATOPROTECTIVE EVALUATION OF EPALTES DIVARICATA (L.) CASS. WHOLE PLANT EXTRACTS AGAINST PARACETAMOL-INDUCED HEPATOTOXICITY IN RATS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i12.14951 ◽

2016 ◽

Vol 8 (12) ◽

pp. 231 ◽

Cited By ~ 1

Author(s):

K. Amala ◽

R. Ilavarasan ◽

R. Arunadevi ◽

S. Amerjothy

Keyword(s):

Aqueous Extract ◽

Histopathological Examination ◽

Hepatoprotective Activity ◽

Hepatoprotective Effect ◽

Dependent Manner ◽

Traditional Use ◽

Whole Plant ◽

Medicinal Use ◽

Histopathological Studies ◽

Dose Dependent

Objective: The plant of Epaltes divaricata (L.) Cass. Traditionally used for jaundice. The present work aimed to investigate the hepatoprotective activity of alcohol and aqueous extract of the whole plant against paracetamol-induced hepatotoxicity in rats to substantiate its traditional use.Methods: The alcohol and aqueous (200 and 400 mg/kg) extract of Epaltes divaricata prepared by cold maceration were administered orally to the animals with hepatotoxicity induced by paracetamol (1000 mg/kg). Silymarine (40 mg/k) was given as reference standard. Hepatoprotective activity was assessed by estimating marker enzymes and by histopathological studies.Results: Both alcohol and aqueous (200 and 400 mg/kg) extract treatment significantly restored the paracetamol-induced elevations in levels of serum enzymes aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphate (ALP) and total bilirubin in a dose-dependent manner. Histopathological examination revealed that the treatment attenuated the paracetamol-induced damage to the liver. The hepatoprotective effect of both extracts was comparable to that of the standard hepatoprotective agent, silymarin.Conclusion: The alcohol and aqueous extract of E. divaricata exhibited hepatoprotective effect against paracetamol-induced liver damage in rats. This study also validated their traditional medicinal use in jaundice.

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The Hepatoprotective Effect of Ethanol Extract of Syzygium campanulatum (Korth) and Syzygium aromaticum (L) Leaf on Male Wistar Rat (Rattus Norvegicus) was Induced by Paracetamol

JURNAL ILMU KEFARMASIAN INDONESIA ◽

10.35814/jifi.v15i2.517 ◽

2017 ◽

Vol 15 (2) ◽

pp. 196

Author(s):

Much Ilham Novalisa Aji Wibowo ◽

Nur Aeni ◽

Zidna Mazayatul Huda ◽

Nunuk Aries Nurulita

Keyword(s):

Wistar Rats ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Hepatoprotective Activity ◽

Group Iv ◽

Control Group ◽

Syzygium Aromaticum ◽

Male Wistar Rats ◽

Hepatoprotective Agent ◽

Better Than

Syzygium campanulatum and Syzygium aromaticum contains antioxidant components suchas flavonoids, phenolic, and terpenoids. May have hepatoprotective properties in reducing SGPT and SGOT activity. This research wants to determine the potency of hepatoprotective of ethanolic extract of Syzygium campanulatum (Korth) and Syzygium aromaticum leaf compared with curcuma tablets. This research uses 24 male Wistar rats divided into 6 groups: I, II, III (as a normal, induction, and compared control), group IV, V, VI were treated 105, 210, and 420 mg/kg BW respectively. The study was conducted for 9 days. After 7 days of treatment, treated groups were exposed by hepatotoxic dose of paracetamol (2000 mg/kg BW). The SGPT and SGOT activity of all groups was measured by enzimatic assay. The result can be concluded that Syzygium campanulatum extract was found to be active as hepatoprotective agent with 210 mg/kg BW dosage (SGPT 21.76 ± 3.98 U/L and SGOT 7.32±6.74U/L) as eff ective as with the curcuma tablets (SGPT 23.91 ± 4.41 U/L and SGOT 14.12±5.37 U/L) and the hepatoprotective activity of Syzygium campanulatum extract at a dosage 420 mg/kg BW better than curcuma tablets (SGPT 12.43 ± 6.51 U/L and SGOT 6.64 ± 5.88 U/L). While the hepatoprotec Syzygium campanulatum and Syzygium aromaticum contains antioxidant components such as flavonoids, phenolic, and terpenoids.May have hepatoprotective properties in reducing SGPT and SGOT activity. This research wants to determine the potency of hepatoprotective of ethanolic extract of Syzygium campanulatum (Korth) and Syzygium aromaticum leaf compared with curcuma tablets. This research uses 24 male Wistar rats divided into 6 groups: I, II, III (as a normal, induction, and compared control), group IV, V, VI were treated 105, 210, and 420 mg/kg BW respectively. The study was conducted for 9 days. After 7 days of treatment, treated groups were exposed by hepatotoxic dose of paracetamol (2000 mg/kg BW). The SGPT and SGOT activity of all groups was measured by enzimatic assay. The result can be concluded that Syzygium campanulatum extract was found to be active as hepatoprotective agent with 210 mg/kg BW dosage (SGPT 21.76 ± 3.98 U/L and SGOT 7.32±6.74U/L) as eff ective as with the curcuma tablets (SGPT 23.91 ± 4.41 U/L and SGOT 14.12±5.37 U/L) and the hepatoprotective activity of Syzygium campanulatum extract at a dosage 420 mg/kg BW better than curcuma tablets (SGPT 12.43 ± 6.51 U/L and SGOT 6.64 ± 5.88 U/L). While the hepatoprotective activity of Syzygium aromaticum extracts eff ective as with curcuma tablets at all dosage variation.

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Rhizophora Mucronata Lam. (Mangrove) Bark Extract Prevents Ethanol-induced Liver Injury, Oxidative Stress and Apoptosis in Swiss Albino Mice

10.21203/rs.3.rs-1132624/v1 ◽

2021 ◽

Author(s):

Chitra Jairaman ◽

Sabine Matou-Nasri ◽

Zeyad I Alehaideb ◽

Syed Ali Mohamed Yacoob ◽

Anuradha Venkataraman ◽

...

Keyword(s):

Oxidative Stress ◽

Liver Injury ◽

Histopathological Examination ◽

Bark Extract ◽

High Dose ◽

Protective Effects ◽

Rhizophora Mucronata ◽

Ethanol Intoxication ◽

Hepatoprotective Effects

Abstract The bark extract of Rhizophora mucronata (BERM) was recently reported for its prominent in vitro protective effects against liver cell line toxicity caused by various toxicants, including ethanol. Here, we aimed to verify the in vivo hepatoprotective effects of BERM against ethanol intoxication. An oral administration of different concentrations (100, 200, and 400 mg/kg) of BERM prior to high-dose ethanol via intraperitoneal injection was performed in mice. On the 7th day, liver and kidney sections were dissected out for histopathological examination. The ethanol intoxication caused large areas of liver necrosis while the kidneys were not affected. Pre-BERM administration decreased ethanol-induced liver injury, as compared to the mice treated with ethanol alone. In addition, the pre-BERM administration resulted in a decrement in the level of ethanol-induced oxidative stress, revealed by a concomitant increase of GSH and a decrease of MDA hepatic levels. The BERM extract also reversed the ethanol-induced liver injury and hepatotoxicity, characterized by the low detection of TNF-α gene expression level and fragmented DNA, respectively. Altogether, BERM extract exerts antioxidative activities and present promising hepatoprotective effects against ethanol intoxication. The identification of the related bioactive compounds will be of interest for future use at physiological concentrations in ethanol-intoxicated individuals.

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