Serum Macro-Minerals Profile and Conception Rate following Heatsynch and Heatsynch + PRID Protocols in Subestrus Surti Buffaloes

Author(s):  
Swati Saxena ◽  
C. T. Khasatiya ◽  
H. R. Savani ◽  
S. B. Patel ◽  
S. S. Chaudhary

A study was carried out to interpret the first and second service conception rate along with serum macro-minerals profile for Heatsynch (T1) and Heatsynch + PRID (T2) protocols with fix timed AI in subestrus Surti buffaloes (n=6 each), keeping one group as untreated control (T3). In Heatsynch protocol, buffaloes were administered i.m. with Inj. Busereline acetate 10 μg on day 0, Inj. Cloprostenol sodium 500 μg on day 7 and Inj. oestradiol benzoate (EB) 0.5 mg on day 8 followed by FTAIs twice on day 10, while in Heatsynch + PRID protocol the buffaloes were inserted with PRID (0.96 g of progesterone) intravaginally for 7 days together with above Heatsynch protocol. Blood samples were collected aseptically in vaccutainers from all these animals on day 0 (prior to treatment), day 4 (during treatment), day 8 (after PGF2α inj.), day of induced estrus/FTAI and on day 28 (post-treatment, day 18 post-AI), and serum samples were stored at –20°C until analyzed. The buffaloes that became pregnant at 1st service (FTAI) in the treatment group T1 and T2 were 50.00 (3/6) and 66.66 (4/6) % and at 2nd cycle 100.00 (3/3) and 50.00 (1/2) %, respectively. The corresponding conception rates for control group T3 were 33.33 (2/6) and 50.00 (2/4) %. The overall pooled CRs of 2 cycles achieved in groups T1, T2 and T3 were 100.00 (6/6), 83.33 (5/6) and 66.66 (4/6) %, respectively. The overall mean serum calcium, phosphorus and magnesium values did not differ significantly (p>0.05) among T1, T2 and T3 groups at any day or within group between different sampling intervals. Heatsynch alone was better and economic over other groups in managing subestrus buffaloes.

Author(s):  
Sandhya S. Chaudhary ◽  
Tanvi D. Manat ◽  
Virendra Kumar Singh ◽  
Sanjay B. Patel

The present study was undertaken to investigate changes in serum hormonal profile of post partum Surti goats. 20 recently parturated (treatment) goats and 20 non pregnant (control group) goats. Blood samples were collected from treatment group on 0, 7, 14, 21, 30 and 45 day post kidding and once from control group. Serum samples were analyzed for Tri-Iodothyronine (T3) and Tetra- Iodothyronine /Thyroxine (T4), cortisol, progesterone (P4) and estradiol, T4 and P4 were significantly low whereas cortisol and estradiol levels were significantly high on 0 day post partum. Onset of kidding was marked by increased cortisol and estrogen and decreased progesterone.


2013 ◽  
Vol 12 (2) ◽  
pp. 93
Author(s):  
Gh.H. Jameel

The aim of this study is investigated the causes of retardation in growth of purebred calves infected by Theileria annulata.Blood samples of infected group (treatment group) were taken once, and blood samples of the control group (clinically healthy) were taken also .Serum separation was done to two groups to determinate some blood factors levels as ferritin ,phosphorus ,glucose ,growth hormone ,total T3 and total T4.It was detected that mean values of serum Ferritin was significantly higher than the values of the control group and healthy animals.No significant effect of the infection on the phosphorus and T3 levels ,While there were significant depression in serum levels of glucose ,growth hormone and T4.


2020 ◽  
Vol 11 (4) ◽  
pp. 5173-5178
Author(s):  
Faehaa Azher Al-Mashhadane

Adenosine is a protective regulator that act endogenously to restore equilibrium of cellular energy in response to tissue trauma. It can perform such function of different systems in the body by activation of adenosine receptors. Study the effects of systemic administration of the adenosine on tongue and salivary glands tissues in the rabbit model. Thirty male rabbits of body weight of 1.5 ± 0.25kg were included in the study. In control group (15 animals), one ml of distilled water was injected intraperitoneally while in treatment group (15 animals) were injected by adenosine intraperitoneally at a dose of one mg/ml, All animals were sacrificed after 30 days. Serum samples were separated and used for analysis of adenosine deaminase (ADA)and glutathione(GSH). Tissue samples sections from tongue and salivary glands were stained with hematoxylin-eosin (H&E) and examined under a light microscope for histological changes by a blinded pathologist. Histological sections in treatment group showed congestion of blood vessels and infiltration of inflammatory cells with mild hemorrhage among acini of salivary glands. Increased level of adenosine in the body microenvironment may affect tongue and salivary glands tissues by modulating some processes including inflammation and blood vessels.


2010 ◽  
Vol 50 (6) ◽  
pp. 503 ◽  
Author(s):  
D. H. Whiteman ◽  
N. W. Tomkins ◽  
R. J. Young ◽  
I. Immig ◽  
G. Weber ◽  
...  

Belmont red and Brahman cows (n = 50) were allocated to one of two groups to determine if β-carotene supplementation could reduce the incidence of mastitis and consequently improve calf productivity from birth to weaning. Both groups received a molasses-based supplement ad libitum; the treatment group supplement was fortified with ROVIMIX® β-carotene and ROVIMIX® E50 whereas the control group were provided access to the molasses supplement only. Blood samples were collected from cows pre and post calving and from calves when 84 ± 0.4 days old to measure plasma β-carotene concentration (PCC). Milk samples were collected 7 and 84 days post calving and at weaning and analysed for somatic cell count (SCC) and composition. Supplementation had no significant (P > 0.05) effect on SCC, calf PCC, calf LW gain and LW at weaning. Supplemented cows had significantly (P < 0.05) higher PCC compared with unsupplemented cows (4.9 ± 0.36 v. 3.9 ± 0.24 mg/L, respectively) at weaning. Cows commenced the study in an above-average condition, and combined with unseasonal green forage it is concluded that β-carotene supplementation has no effect on mastitis or calf weaning weight.


Author(s):  
B.V.E. Segwagwe ◽  
J. Malmo ◽  
K.L. Macmillan ◽  
P.D. Mansell

This experiment compared the reproductive performance of synchronised anoestrous dairy cows that were treated initially with a combination of progesterone and oestradiol benzoate and then with either gonadotrophin-releasing hormone (GnRH) or oestradiol benzoate to resynchronise returns to service. It was hypothesised that injecting anoestrous dairy cows with GnRH 12-15 days after insemination and coinciding with the time of insertion of a controlled intravaginal progesterone-releasing (CIDR) device would increase conception rates to the preceding 1st insemination compared with oestradiol benzoate treated cows; both GnRH and oestradiol benzoate would resynchronising the returns to service of those cows that did not conceive to the preceding insemination. Groups of cows in 11 herds were presented for a veterinary examination after they had not been seen in oestrus postpartum. Those cows diagnosed with anovulatory anoestrus (n = 1112) by manual rectal palpation and / or ultrasonography were enrolled in the trial. Each enrolled cow was injected with 2mg oestradiol benzoate i.m. on Day -10, (where Day 0 was the 1st day of the planned insemination) concurrently with vaginal insertion of a CIDR device. The device inserted was withdrawn on Day -2 and then each cow injected i.m. with 1 mg of oestradiol benzoate on Day -1 unless it was in oestrus. Observation for oestrus preceded each insemination. Every cow that had been inseminated on Days -1,0,1 or 2 was presented for treatment for resynchrony on Day 14 (n=891). They were divided into 2 groups; those with an even number were each injected i.m. with 250 µg of a GnRH agonist (Treatment group n = 477); each of the cows with an odd number injected i.m. with 1mg of oestradiol benzoate (control group, n = 414). Each GnRH or oestradiol benzoate injection preceded reinsertion of a CIDR device previously inserted from Days -10 to -2. It was withdrawn on Day 22, 24 hours before injecting 1mg oestradiol benzoate. Cows observed in oestrus were submitted for a 2nd insemination. Every enrolled cow still present in the herd was pregnancy tested by palpation of uterine contents per rectum about 6 weeks later and again at the end of a herd's seasonal breeding programme. The alternative use of GnRH instead of oestradiol benzoate did not affect the percentage of cows conceiving within 3 days of the mating start date (MSD) (35.6 %vs 35.3 %, P=0.90), resubmission rates for a 2nd insemination among cows not pregnant to the 1st insemination (81.6 % vs 83.5 %, P=0.41), 6-week pregnancy rate (59.3 % vs 60.6 %, P=0.65), 21-week pregnancy rate (86.6 vs 85.0, P=0.36), mean interval from MSD to conception (32.5 + 1.8 days vs 29.9 + 1.8 days, P = 0.26) or conception rate of cows reinseminated by Day 28 (43.3 % vs 38.8 %, P=0.39). When GnRH conception rate of cows reinseminated by Day 28 (43.3% vs was compared with oestradiol benzoate, it did not increase conception rates to the 1st service; it was as effective as oestradiol benzoate in synchronising returns to service in previously treated anoestrous cows that did not conceive to the 1st service. Its use affected neither conception rates to the preceding 1st inseminations nor to the following 2nd inseminations.


1986 ◽  
Vol 43 (2) ◽  
pp. 341-349 ◽  
Author(s):  
Philippa R. Eastham ◽  
G. W. Dyck ◽  
D. J. A. Cole

AbstactForty-eight Landrace × (Landrace × Large White) gilts were randomly allocated at 70 days of age to each of three rearing treatments: (1) fence-line contact with a mature boar, (2) olfactory/aural contact with a mature boar, (3) no boar contact. At 160 days of age, all gilts were relocated adjacent to a novel mature boar and exposed to full contact with a mature boar for 30 min each day. At the first, second and third oestrus, 16 gilts from each treatment group were mated twice and the reproductive tracts were examined at slaughter 21 days later, to determine ovulation rate and embryo survival. The rearing treatments had no significant effects on the attainment of puberty. Neither rearing treatment nor oestrus of mating had any significant effect on behaviour, sexual receptivity at first service, conception rate, ovulation rate, the number of embryos and embryo survival at 21 days of pregnancy.


2021 ◽  
Vol 15 (3) ◽  
pp. 261-266
Author(s):  
Claudia Dias Monteiro-Toma ◽  
Hugo Shisei Toma ◽  
Lívia Saab Muraro ◽  
Eunice Oba ◽  
Jéssica Núbia da Silva Almeida ◽  
...  

Few information is available about the optimal period for colostrum absorption in the bowel of newborn lambs. The aim of the current study was to investigate the effects of using supplementation with commercial core homeopathic during pregnancy and early lactation on 18 pregnant Santa Inês ewes presenting body condition score ≥ 2.5. Pregnant animals were randomly divided in two groups; one was used as control (control group) and the other was supplemented on a daily basis with 30 g of homeopathic product with Natrum muriaticum 10-60, Calcium carbonicum 10-30, Silicea terra 10-400 and Hypothalamus 10-30 per animal, mixed to the concentrate during pregnancy and early lactation (treatment group). The Total Protein (TP) and globulin (GLB) values found in ewes and lambs belonging to the treatment group were higher than and different from those recorded for the control group. The serum albumin (ALB) concentration did not differ between groups of ewes but was different in lambs. TP and GLB evolution in lambs and ewes throughout the seven experimental days showed the animals in the treatment group trend to present higher TP and GLB values in all experimental periods; supplemented animals showed trend to progressive increase in both variables until day 3; whereas non-supplemented animals showed such trend until day 2 (when they reached their peak). The commercial core homeopathic to the animals during pregnancy and early lactation resulted in higher TP and GLB concentrations in the serum samples collected from ewes and lambs.


2020 ◽  
Vol 57 (1) ◽  
pp. 78-82 ◽  
Author(s):  
R. A. Papini ◽  
C. Orsetti ◽  
M. Sgorbini

SummaryThe study evaluated the effectiveness of a commercially available polyherbal dewormer to control intestinal strongyles in naturally infected donkeys. The animals were allotted to two groups: treated with the herbal dewormer (n=8) according to manufacturer recommendations and untreated control group (n=6). Fecal samples were taken from each animal on days 0 (day of treatment), 14, 21 (day of second additional treatment), 35, and 42. Faecal egg count reduction tests showed very negligible or no reduction in number of strongyle eggs for donkeys in the phytotherapeutic treatment group compared to those in the control group. Thus, the herbal dewormer was mostly ineffective in reducing fecal egg counts in donkeys infected with intestinal strongyles. These findings can make equine practitioners aware of possible disadvantages of herbal dewormers.


2019 ◽  
Vol 40 (6Supl2) ◽  
pp. 3057
Author(s):  
Matheus Gomes Lopes ◽  
José Henrique Echenique Dominguez ◽  
Cristina Mendes Peter ◽  
Ederson Santos ◽  
Paula Almeida Rodrigues ◽  
...  

The aim of this study was to evaluate the humoral immune response in beef heifers supplemented with mineral supplementation with or without the addition of rumen-protected methionine. Forty-eight Brangus nulliparous heifers were distributed into four experimental groups with three replications each: control group without supplementation and without vaccination (CG01), control group without supplementation and with vaccination (CG02), treatment group with mineral supplementation and vaccination (TG01), and treatment group with mineral supplementation added with protected methionine and vaccination (TG02). The animals were maintained under native pasture with access to water ad libitum and the supplementation was available in high-consumption covered troughs. A supplementation period of 60 days prior to vaccinations was adopted until the first dose of a monovalent experimental vaccine inactivated for BoHV-5 was applied as a method of stimulating the immune response to evaluate the supplementation effects. After a 21-day interval, blood samples were collected to evaluate the humoral response and the second vaccine booster dose was applied following the 21-day interval for new blood samples in order to evaluate the immune response against the two-vaccination protocol. From the beginning of the experiment, the animals were weighed on the days ?60, ?10, 0, 21, and 42 in relation to the vaccine protocol. The experimental groups did not differ for body weight, mean daily weight gain, and body condition score after 102 days of supplementation regardless of the treatment. No animals belonging to CG01 seroconverted throughout the experiment, proving that there was no introduction of the agent (BoHV) in the studied area. When vaccinated animals were compared to the CG01 control group, statistically higher levels of neutralizing antibodies (P ? 0.0001) and IgG (P ? 0.0001) were verified 21 days after the second vaccine dose. Among the animals of the three vaccinated groups, there was no difference in seroconversion and IgG production. Therefore, no benefits of mineral supplementation or enriched with protected methionine were observed for the humoral immune response of the studied animals.


2016 ◽  
Vol 86 (6) ◽  
pp. 934-942 ◽  
Author(s):  
Asli Baysal ◽  
Mehmet Ali Ozturk ◽  
Ahmet Oguz Sahan ◽  
Tancan Uysal

ABSTRACT Objective: To evaluate three-dimensional (3-D) soft tissue facial changes following rapid maxillary expansion (RME) and to compare these changes with an untreated control group. Materials and Methods: Patients who need RME as a part of their orthodontic treatment were randomly divided into two groups of 17 patients each. Eligibility criteria included having maxillary transverse deficiency with crossbite, and to be in the normal range according to body mass index. In the first group (mean age  =  13.4 ± 1.2 years), expansion was performed. The second group received no treatment initially and served as untreated control (mean age  =  12.8 ± 1.3 years). Skeletal and soft tissue changes were evaluated using posteroanterior cephalograms and 3-D facial images. The primary outcome of this study was to assess the soft tissue changes. The secondary outcomes were evaluation hard tissue and soft tissue relations. Randomization was done with preprepared random number tables. Blinding was applicable for outcome assessment only. MANOVA, t-test, and correlation analyses were used (P  =  .05). Results: In both groups, there was a general trend of increase for the transverse skeletal measurements, but these increases were more limited in the control group. Alar base width was greater in the treatment group (P  =  .002). Pogonion soft tissue point (P  =  .022) was located more posteriorly in the expansion group compared with the control group. Conclusions: Soft tissue changes between groups were similar, except for the alar base, which became wider in the treatment group. Weak correlations were found between the skeletal and soft tissue changes.


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