scholarly journals Histochemistry of backcross 1 of oil palm seeds at different storage periods

2020 ◽  
Vol 5 (1) ◽  
pp. 52
Author(s):  
Ernayunita Ernayunita ◽  
Taryono Taryono ◽  
Prapto Yudono
Keyword(s):  
Oil Palm ◽  
Oil Quality ◽  
Seed Storage ◽  
Fat Content ◽  
Endosperm Tissue ◽  
Seed Deterioration ◽  
Seed Content ◽  
Deterioration Process ◽  
Histochemical Tests ◽  
Food Reserves

Oil palm backcross 1 is the result of the crossing between E. oleifera and E. guineensis, followed by backcrossing to one of its parents. It has several advantages, including slow height growth and good oil quality, thereby having the potential to be developed. However, it also has a short seed shelf life, which might be inherited from E. oleifera that has relatively quick seed deterioration. This is problem to the breeding program, and there have not been many studies on the seed deterioration process. A histological examination can determine the composition of food reserves in seed endosperm tissue. Therefore, it is necessary to assess the histochemistry of seeds concerning the process of seed deterioration. Histochemical tests with Sudan III, Milon, and IKI reagents were used as histochemical tests of fat, protein and carbohydrate seed content with different seed storage periods, i.e. 4, 3, 2, and less than 1 year. The result showed that the fat content decreased during the storage periods. Based on carbohydrates and proteins staining, there were only very few substances , which were difficult to distinguish among the storage periods, so that this analysis could not be used as the determining indicator of seed deterioration. The fat content was a determining factor of seed deterioration and quality. The oil palm seed storage up to 3-4 years led to the reduction in the fat content in significant quantities compared to the fresh seeds stored less than 1 year as they still contained a lot of fat in the cell.

Accelerated Ageing Mediated Seed Longevity Prediction and Assessment of Seed Deterioration Pattern through 2D-Gel Electrophoresis in Chickpea (Cicer arietinum L.)

2021 ◽  
Author(s):  
Shivasharanappa S. Patil ◽  
Ashok S. Sajjan ◽  
N.K. Biradarpatil ◽  
P.U. Krishnaraj ◽  
I.S. Katageri
Keyword(s):  
Seed Storage ◽  
Quality Parameters ◽  
Seed Longevity ◽  
Accelerated Ageing ◽  
Natural Ageing ◽  
Ambient Conditions ◽  
Seed Deterioration ◽  
Germination Potential ◽  
Deterioration Process ◽  
Ageing Methods

Background: Accelerated ageing is a method to assess seed storage performance by exposing the seeds to higher temperature and relative humidity to make seeds lose its vigour and viability quickly. The seed physiological parameters are then compared with the natural ageing to derive certain conclusions. But, the molecular pattern of seed deterioration under accelerated ageing varies compared to that of natural ageing.Methods: Chickpea, seed lots of variety JG-11 and Annigeri-1 were subjected to natural ageing in cloth bag with nine per cent initial seed moisture under ambient conditions. Simultaneously, representative sample of same seed lot were also aged at 41oC and 95±2% humidity up to 120 hours.Result: Both the ageing methods had significant negative effect on seed physiological and biochemical quality parameters. The germination potential of seeds tested after 48 hours of accelerated ageing (83.5%) was equal 10 months of natural ageing (85%) in JG-11, while also in Annigeri-1, 48 hours of accelerated ageing (78%) was nearly equal to 10 months of natural ageing (80%). The protein expression analyzed through 2D-PAGE at similar germination potential brought out by two independent ageing methods showed higher protein down regulation ratio (3.4) in accelerated ageing than in natural ageing (3.2) unveiling its rapidity in the seed deterioration process. Therefore, accelerated ageing can be used for predicting chickpea seed longevity.

scholarly journals Postharvest Fruit Detachment in Oil Palm Bunches with Ethephon and Ethylene Gas Application

Agriculture ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1030
Author(s):  
Arutchelvam Balakrishnan ◽  
Mohd Ibnur Syawal Zakaria ◽  
Bee Aik Tan ◽  
Jaime Yoke Sum Low ◽  
Shwu Fun Kua ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Palm Oil ◽  
Oil Palm ◽  
Room Temperature ◽  
Indirect Method ◽  
Production Systems ◽  
Oil Quality ◽  
Crude Palm Oil ◽  
Fresh Fruit Bunches

The processing of oil palm fresh fruit bunches (FFB), together with loose fruits, in the current mill operation contributes to oil loss and high free fatty acids (FFA), affecting crude palm oil quality. Fruit detachment induced by ethephon and ethylene may mitigate the current processing issues. This study shows that a 0.50% (v/v) ethephon application by the evaporation method induced the highest fruit detachment of 30.8 ± 1.1% after 24 h at room temperature, with the FFA content in the extracted crude palm oil at 0.34 ± 0.09%. Ethephon application was effective on bunches between 14 and 28 kg, and fruit detachment was higher in ripe and underripe bunches at 24.1 ± 0.9% and 23.2 ± 0.1%, respectively. A significant fruit detachment of 47.2 ± 2.4% was achieved when the bunches were also stripped mechanically, but the FFA content increased almost 4-fold, from 1.0 ± 0.2% to 3.8 ± 1.2%. The application of ethylene gas at 750 ppm yielded 29.4 ± 1.9% fruit detachment. The findings present the possibility of using ethylene as an indirect method for minimizing oil loss without increasing the FFA content in future crude palm oil production systems.

scholarly journals High Drying Temperature Accelerates Sunflower Seed Deterioration by Regulating the Fatty Acid Metabolism, Glycometabolism, and Abscisic Acid/Gibberellin Balance

2021 ◽  
Vol 12 ◽  
Author(s):  
Yutao Huang ◽  
Min Lu ◽  
Huaping Wu ◽  
Tiyuan Zhao ◽  
Pin Wu ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Fatty Acid ◽  
Sunflower Seed ◽  
Acid Metabolism ◽  
Seed Storage ◽  
Seed Vigor ◽  
Sunflower Seeds ◽  
Storage Process ◽  
Drying Temperature ◽  
Seed Deterioration

Sunflower seed storage is accompanied by the loss of seed vigor. Seed drying is a key link between seed harvest and seed storage; however, to date, the effect of seed drying on sunflower seed deterioration during storage remains unclear. The present study performed hot air drying for sunflower seeds with an initial moisture content of 30% to examine the manner in which drying temperature (35, 40, 45, 50, and 55°C) affects the drying performance and seed vigor following storage process (6 and 12 months). A drying temperature of 40°C was evidently safe for sunflower seeds, whereas the high drying temperatures (HTD, 45, 50, and 55°C) significantly lowered sunflower seed vigor by regulating the fatty acid metabolism, glycometabolism, and abscisic acid (ABA)/gibberellin (GA) balance. HDT significantly increased the seed damage rate and accelerated sunflower seed deterioration during natural and artificial aging process. Further biochemical analysis indicated that HDT significantly increased lipoxygenase and dioxygenase activities, leading to malonaldehyde and reactive oxygen species over-accumulation during storage. During early seed germination, HDT significantly inhibited fatty acid hydrolysis and glycometabolism by decreasing triacylglycerol lipase, CoA-SH oxidase, and invertase activities. Moreover, HDT remarkably increased ABA levels but reduced GA levels by regulating gene expressions and metabolic enzyme activities during early imbibitions. Cumulatively, the seed drying effect on sunflower seed vigor deterioration during the storage process may be strongly related to fatty acid oxidation and hydrolysis metabolism, toxic substance accumulation, and ABA/GA balance.

scholarly journals Using Seed Volatiles as a Possible Indicator for Seed Deterioration during Storage

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 526D-526 ◽  
Author(s):  
Ming Zhang ◽  
Eric E. Roos
Keyword(s):  
Volatile Compounds ◽  
Seed Storage ◽  
Storage Conditions ◽  
Moisture Level ◽  
Seed Moisture Content ◽  
Storage Container ◽  
Seed Deterioration ◽  
Seed Moisture ◽  
Dry Conditions ◽  
Moisture Conditions

All kinds of plant seeds evolve volatile compounds during storage. However, a reliable deterioration forecast method is still not established using volatile evolution, even though some preliminary work indicated a relationship between volatile evolution and seed deterioration (Fielding and Goldsworthy, 1982; Hailstones and Smith, 1989; Zhang et al., 1993). Here we review some of the previous work concerning seed volatiles and present some more recent research on the effects of seed moisture content on deterioration. We found that volatile evolution from seeds was controlled by seed moisture level. Generally, seeds tended to evolve more hexanal and pentanal under extremely dry conditions (below 25% equilibrium RH). The production of hexanal and pentanal decreased with increasing seed moisture level. On the other hand, methanol and ethanol increased with increasing seed moisture. All of the volatile compounds accumulated in the headspace of the seed storage container during storage. Therefore, it should be possible to use different volatiles to indicate the deterioration of seeds stored under different moisture levels. We suggest that hexanal may be used for seed assessing deterioration under dry storage conditions (below 25% equilibrium RH), while ethanol may be used for seeds stored under higher moisture conditions (above 25% equilibrium RH). [References: Fielding, J.L. and Goldsworthy, A. (1982) Seed Sci. Technol. 10: 277–282. Hailstones, M.D. and Smith, M.T. (1989) Seed Sci. Technol. 17: 649–658. Zhang et al. (1993) Seed Sci. Technol. 21:359–373.]

scholarly journals SEED DETERIORATION PATTERN OF FOUR BAMBARA GROUNDNUT LANDRACES (Vigna subterraneasubterranean (L) Verdc) IN OPEN STORAGE SYSTEM

2018 ◽  
Vol 27 (2) ◽  
pp. 111
Author(s):  
Happy Suryati Hamami ◽  
Abdul Qadir ◽  
Satriyas Ilyas ◽  
Bambang Budhianto
Keyword(s):  
Storage System ◽  
Seed Storage ◽  
Aluminum Foil ◽  
Equation Model ◽  
Bambara Groundnut ◽  
Vigna Subterranea ◽  
Seed Technology ◽  
Seed Deterioration ◽  
Open Storage ◽  
Rate Of Decline

Bambara groundnut (Vigna subterranea (L.) Verdc) is a potential commodity to be developed in Indonesia, however, the production is done only once a year, therefore, it needs proper seed storage. The aim of this research was to study seed deterioration patterns of four bambara groundnut landraces stored in packages with different permeability in an open storage system for up to 6 months. This experiment was conducted from November 2015 to July 2016 at Seed Technology Laboratory, Department of Agronomy and Horticulture, IPB. Stages of experiment as follow: calculation of packaging permeability, seed storage, preparation and fitting data to regression equation.The packaging used in the study is aluminum foil, pp plastic and plastic sacks which have measured its permeability. The result showed that the seed deterioration of four bambara groundnut landraces in three packaging permeability has common sigmoidpattern with equation model: y = a / {1 + exp ((x + b) / c)}. The seed deterioration pattern based on SG and EC variables with the faster rate of decline occurred in Sumedang landrace packed in plastic sack (permeability = 1.4681 g/day m2 mm/Hg), thus having a shorter storability. The slower rate of decline occurred in Gresik landrace packed in aluminum foil (permeability = 0.098 g/day m2 mm/Hg), this means that it has a longer storability.

Detection of significant SNP associated with production and oil quality traits in interspecific oil palm hybrids using RARSeq

Plant Science ◽  
2020 ◽  
Vol 291 ◽  
pp. 110366 ◽  
Author(s):  
Maider Astorkia ◽  
Mónica Hernández ◽  
Stéphanie Bocs ◽  
Kevin Ponce ◽  
Olga León ◽  
...  
Keyword(s):  
Oil Palm ◽  
Oil Quality ◽  
Quality Traits

Microwave Sterilization of Oil Palm Fruits: Effect of Power, Temperature and D-value on Oil Quality

2013 ◽  
Vol 2 (3) ◽  
pp. 153-156 ◽  
Author(s):  
Maya Sarah ◽  
Mohd. Rozainee Taib
Keyword(s):  
Oil Palm ◽  
Oil Quality ◽  
Microwave Sterilization ◽  
Palm Fruits ◽  
D Value

scholarly journals Brief Review on the Controversies around Oil Palm (Elaeis Guineensis Jacq.) Production and Palm Oil Consumption

2016 ◽  
Vol 3 (2) ◽  
pp. 60 ◽  
Author(s):  
Godswill Ntsomboh Ntsefong ◽  
Hermine Ngalle-Bille ◽  
Walter Ajambang ◽  
Benoit Constant Likeng-Li-Ngue ◽  
Tabi-Mbi Kingsley ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Palm Oil ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Saturated Fatty Acids ◽  
Oil Quality ◽  
Human Consumption ◽  
Oil Consumption ◽  
Oil Palm Industry ◽  
Environmentally Friendly Production

Palm oil from <em>Elaeis guineensis</em> Jacq. of the Arecaceae family is the main traditional cooking oil in most parts of tropical Africa. Oil palm cultivation requires large areas of land and the consumption of crude palm oil from it raises health concerns notably with regards to the incidence of cardiovascular disease and obesity. The industry has therefore been faced with two major controversies. Firstly, palm oil which contains about 50% saturated fatty acids is considered to be unhealthy for human consumption because its saturated fatty acids raise blood LDL cholesterol level and increase the risk of the above mentioned diseases. Secondly, due to its land intensive nature, the oil palm industry is linked to deforestation, climate change and socioeconomic instability. For these reasons, the oil palm industry has been at the forefront of environmental and reputational challenges. This paper briefly presents an overview of these controversies surrounding the oil palm industry, while highlighting the possible outlets for environmentally friendly production processes and improvement strategies for better oil quality.

scholarly journals High-Oleic Palm Oil (HOPO) Production from Parthenocarpic Fruits in Oil Palm Interspecific Hybrids Using Naphthalene Acetic Acid

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 290
Author(s):  
Hernán Mauricio Romero ◽  
Edison Daza ◽  
Iván Ayala-Díaz ◽  
Rodrigo Ruiz-Romero
Keyword(s):  
Acetic Acid ◽  
Palm Oil ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Fatty Acid Content ◽  
Oil Quality ◽  
Naphthalene Acetic Acid ◽  
Elaeis Oleifera ◽  
High Oleic ◽  
Seedless Fruits

Interspecific OxG hybrids of African palm Elaeis guineensis Jacq. and the American palm Elaeis oleifera Cortes produce high-oleic palm oil (HOPO) with low saturated fatty acid content. OxG hybrids are highly productive, grow slowly, and are resistant to bud rot disease. However, OxG hybrid pollen presents low viability and germinability, so assisted pollination is a must. Hybrids can produce parthenocarpic or seedless fruits, with the exogenous application of plant growth regulators. Thus, naphthalene acetic acid (NAA) effects on parthenocarpic fruits induction, bunch formation, and oil quality were evaluated. The OxG hybrid Coari x La Mé was used. NAA doses, frequency, number of applications, and the phenological stages for the treatments were defined. A total dose of 1200 mg L−1 NAA applied three or four times produced bunches with better fruit set, similar average bunch weight, and oil to dry mesocarp than those obtained with assisted pollination. At a semi-commercial scale, 1200 mg L−1 NAA induced bunches that consisted of 93% or more of seedless fruits. Bunch number (2208 ± 84 versus 1690 ± 129) and oil to bunch (32.2 ± 0.7 versus 25.3 ± 0.8) were higher in the NAA induced bunches than in the assisted pollination. However, the average bunch weight was lower (12.2 ± 0.4 versus 14.9 ± 0.6). NAA increased oil to bunch in 36% (8.7 ± 0.1 versus 6.4 ± 0.3). Thus, with this technology, it is plausible to reach more than 10 tons per hectare per year of HOPO. Potentially, without increasing the planted oil palm area, OxG hybrids and NAA applications could alone meet the world’s fats and oil demands.

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