scholarly journals PERBANDINGAN GAMBARAN DARAH ULAR SANCA BATIK (Malayophyton reticulatus) LOKAL JAWA DAN KALIMANTAN

2019 ◽  
Vol 37 (1) ◽  
pp. 121
Author(s):  
Slamet Raharjo ◽  
Sri Hartati ◽  
Sedarmanto Indarjulianto ◽  
Rini Widayanti
Keyword(s):  
Veterinary Medicine ◽  
Health Status ◽  
Blood Sample ◽  
Animal Health ◽  
Animal Blood ◽  
Blood Profile ◽  
Blood Samples

Reticulated python (M. reticulatus) Java and Kalimantan locality highly demand as pet animal. Blood profile has an important role on animal health status. This research was aim to study the blood profile comparison of Java and Kalimantan M. reticulatus. Ten blood samples of Java M. reticulatus and 10 samples of Kalimantan M. reticulatus were used in this study. As much as 1 ml blood sample was collected from ventral coccygeal vein of adult healthy M. reticulatus. Blood sample put into tube with EDTA then analized at Internal Department Laboratory, Faculty of Veterinary Medicine UGM. Based on blood samples analize results, adult healthy Java M. reticulatus shows the higher value on parameters of erytrocyte, leucocyte, PCV, MCH, heterophyl, limphocyte and monocyte, while haemoglobin, TPP, MCV, MCHC, azurofil and monosit were lower than Kalimantan M. reticulatus. There were no significant differences (P>0,05) between blood profile of Java and Kalimantan M. reticulatus. It could be concluded that blood profile of Java and Kalimantan M. reticulatus is not different.

scholarly journals Impact of Cadmium Intoxication on Health Status, Rumen and Blood Constituents in Egyptian Ossimi Sheep

2021 ◽  
Vol 10 (2) ◽  
pp. 102-106
Keyword(s):  
Drinking Water ◽  
Health Status ◽  
Rumen Fluid ◽  
Animal Health ◽  
Ammonia Nitrogen ◽  
Strong Positive Correlation ◽  
Toxic Heavy Metals ◽  
Animal Blood ◽  
Antioxidant Markers ◽  
The Impact

Toxic heavy metals particularly Cadmium (Cd) have a hazardous impact on animal health and productivity because of their ill-degradability and bio-accumulation for long periods. This study carried out on 52 Ossimi sheep belonging to Giza Governorate, including 12 sheep considered as control (kept in a private farm) and 40 sheep grazed on an area polluted with cadmium. Drinking water, animal blood and rumen fluid samples were collected from all sheep. Complete blood, rumen and serum constituents were analyzed. Iron, copper, zinc, oxidant, and antioxidant markers were evaluated and Cd levels in water, rumen fluid and serum were investigated to show the impact of Cd on those parameters. Physical examination revealed significant disturbance in health status of Cd-exposed sheep. Rumen fluid examination showed significant increase in rumen pH, significant decrease in rumen ammonia-nitrogen, TVFAs, AST, ALT, GGT, Ca and Ph. Blood constituent revealed significant alteration as significant decrease in RBCs count, Hb, PCV, MCHC and TLC as well as impaired hepatic and renal function and significant decrease of antioxidant markers. These alterations associated with strong positive correlation between these altered parameters and cadmium level in drinking water, rumen fluid and blood samples which markedly increased more than permissible limits; these results should be put in consideration in interpretation of affected animals' status and during treatment and control of cadmium exposed sheep cases as well as consumption of such animals' meat and offal is not recommended.

scholarly journals Development of a blood sample detector for multi-tracer positron emission tomography using gamma spectroscopy

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Carlos Velasco ◽  
Adriana Mota-Cobián ◽  
Jesús Mateo ◽  
Samuel España
Keyword(s):  
Positron Emission Tomography ◽  
Blood Sample ◽  
Pet Imaging ◽  
Spectroscopic Analysis ◽  
Gamma Spectroscopy ◽  
Emission Tomography ◽  
Blood Samples ◽  
Positron Emission

Abstract Background Multi-tracer positron emission tomography (PET) imaging can be accomplished by applying multi-tracer compartment modeling. Recently, a method has been proposed in which the arterial input functions (AIFs) of the multi-tracer PET scan are explicitly derived. For that purpose, a gamma spectroscopic analysis is performed on blood samples manually withdrawn from the patient when at least one of the co-injected tracers is based on a non-pure positron emitter. Alternatively, these blood samples required for the spectroscopic analysis may be obtained and analyzed on site by an automated detection device, thus minimizing analysis time and radiation exposure of the operating personnel. In this work, a new automated blood sample detector based on silicon photomultipliers (SiPMs) for single- and multi-tracer PET imaging is presented, characterized, and tested in vitro and in vivo. Results The detector presented in this work stores and analyzes on-the-fly single and coincidence detected events. A sensitivity of 22.6 cps/(kBq/mL) and 1.7 cps/(kBq/mL) was obtained for single and coincidence events respectively. An energy resolution of 35% full-width-half-maximum (FWHM) at 511 keV and a minimum detectable activity of 0.30 ± 0.08 kBq/mL in single mode were obtained. The in vivo AIFs obtained with the detector show an excellent Pearson’s correlation (r = 0.996, p < 0.0001) with the ones obtained from well counter analysis of discrete blood samples. Moreover, in vitro experiments demonstrate the capability of the detector to apply the gamma spectroscopic analysis on a mixture of 68Ga and 18F and separate the individual signal emitted from each one. Conclusions Characterization and in vivo evaluation under realistic experimental conditions showed that the detector proposed in this work offers excellent sensibility and stability. The device also showed to successfully separate individual signals emitted from a mixture of radioisotopes. Therefore, the blood sample detector presented in this study allows fully automatic AIFs measurements during single- and multi-tracer PET studies.

scholarly journals Tick-borne zoonoses and commonly used diagnostic methods in human and veterinary medicine

2021 ◽  
Author(s):  
Andrea Springer ◽  
Antje Glass ◽  
Julia Probst ◽  
Christina Strube
Keyword(s):  
Veterinary Medicine ◽  
Diagnostic Tests ◽  
One Health ◽  
Animal Health ◽  
Diagnostic Techniques ◽  
Diagnostic Methods ◽  
Diagnostic Tools ◽  
Zoonotic Pathogens ◽  
Health Concept ◽  
The One

AbstractAround the world, human health and animal health are closely linked in terms of the One Health concept by ticks acting as vectors for zoonotic pathogens. Animals do not only maintain tick cycles but can either be clinically affected by the same tick-borne pathogens as humans and/or play a role as reservoirs or sentinel pathogen hosts. However, the relevance of different tick-borne diseases (TBDs) may vary in human vs. veterinary medicine, which is consequently reflected by the availability of human vs. veterinary diagnostic tests. Yet, as TBDs gain importance in both fields and rare zoonotic pathogens, such as Babesia spp., are increasingly identified as causes of human disease, a One Health approach regarding development of new diagnostic tools may lead to synergistic benefits. This review gives an overview on zoonotic protozoan, bacterial and viral tick-borne pathogens worldwide, discusses commonly used diagnostic techniques for TBDs, and compares commercial availability of diagnostic tests for humans vs. domestic animals, using Germany as an example, with the aim of highlighting existing gaps and opportunities for collaboration in a One Health framework.

scholarly journals Microsphere-Based Microfluidic Device for Plasma Separation and Potential Biochemistry Analysis Applications

Micromachines ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 487
Author(s):  
Hongyan Xu ◽  
Zhangying Wu ◽  
Jinan Deng ◽  
Jun Qiu ◽  
Ning Hu ◽  
...  
Keyword(s):  
Blood Sample ◽  
Microfluidic Device ◽  
Biochemical Analysis ◽  
Cost Effective ◽  
Clinical Diagnostics ◽  
Correction Factors ◽  
Separation Device ◽  
Blood Samples ◽  
Plasma Separation ◽  
Blood Biochemical

The development of a simple, portable, and cost-effective plasma separation platform for blood biochemical analysis is of great interest in clinical diagnostics. We represent a plasma separation microfluidic device using microspheres with different sizes as the separation barrier. This plasma separation device, with 18 capillary microchannels, can extract about 3 μL of plasma from a 50 μL blood sample in about 55 min. The effects of evaporation and the microsphere barrier on the plasma biochemical analysis results were studied. Correction factors were applied to compensate for these two effects. The feasibility of the device in plasma biochemical analysis was validated with clinical blood samples.

Note on the regulation of veterinary medical devices in the EU: A review of the current situation and its impact on animal health and safety

2020 ◽  
Vol 29 (1) ◽  
pp. 37-43
Author(s):  
H Potockova ◽  
P Kusnierik ◽  
J Dohnal
Keyword(s):  
Veterinary Medicine ◽  
Medical Devices ◽  
Animal Health ◽  
Health And Safety ◽  
Well Being ◽  
Current Situation ◽  
Safety And Efficacy ◽  
Safety Risks ◽  
Health And Safety Risks ◽  
The Eu

Medical devices form a large heterogeneous group of products ranging from simple tools to medical testing and implants, the safety and efficacy of which are strictly regulated in all developed countries. Thanks to the health and cost benefits, medical devices have also found their way into veterinary medicine but, surprisingly, the regulation of these products is far less complex or, in some cases, missing altogether. Given the complexity and potential hazards of certain veterinary devices, the current state of affairs may lead to health and safety risks, both for animals and personnel involved. This review is the first to systematically map the current situation in the EU, revealing health and safety risks in practice for both animals and personnel involved and discussing them in a broader context. Only six out of the EU's 28 member states (Belgium, Croatia, Czech Republic, Germany, Hungary, and Slovakia) were found to have at least a degree of regulation of veterinary devices. As a result, a single product may be regulated as a veterinary medicinal product, a veterinary medical device or not be regulated at all, depending on the particular EU member state in question. As things stand, veterinary medicine makes use of all kinds of medical devices, including human products, regardless of their regulatory status and (pre-market) control. However, the use of such devices may influence the health and well-being of animals. Several measures are therefore suggested to attain the required levels of safety and efficacy surveillance for veterinary medical devices without creating excessive administration.

scholarly journals Mechanisms of quinolone resistance and implications for human and animal health

2010 ◽  
Vol 64 (3-4) ◽  
pp. 277-285
Author(s):  
Maja Velhner ◽  
Gordana Kozoderovic ◽  
Zora Jelesic ◽  
Igor Stojanov ◽  
Radomir Ratajac ◽  
...  
Keyword(s):  
Veterinary Medicine ◽  
Antimicrobial Resistance ◽  
Protein Interactions ◽  
Bacterial Infections ◽  
Animal Health ◽  
Point Mutations ◽  
Inhibitory Effect ◽  
Quinolone Resistance ◽  
Topoisomerase Iv ◽  
Target Enzyme

Quinolone antibiotics have been widely used in human and veterinary medicine. This has caused the development of resistance and difficulties in the treatment of complicated bacterial infections in humans. The resistance to quinolones develops due to chromosome mutations and it can also be transferred by plasmids. The target enzyme for quinolones in Gram-negative bacteria is Gyrasa A, while the target enzyme in Grampositive bacteria is mostly topoisomerase IV. Gyrase A consists of two subunits encoded by genes gyrA and gyrB. The function of the enzyme is to introduce negative super coiling in DNA and therefore is essential for the replication of bacteria. Quinolone resistance develops if point mutations at 83 and/or 87 codon are introduced on gyrA. Establishing a minimal inhibitory concentration (MIC) to this group of antimicrobials will reveal possible mutations. Recently it was discovered that quinolone resistance is transmittable by plasmid termed PMQR (plasmid mediated quinolone resistance). The target gene marked qnr encodes a pentapeptide repeat family protein. Pentapeptide repeats form sheets, involved in protein-protein interactions. Qnr protein binds to GyrA protecting the enzyme from the inhibitory effect of ciprofloxacin. The distribution of qnr related resistance is higher in humans than in animals. In poultry, however, this type of resistance is present more than in other animals. Plasmid mediated resistance contributes to the faster spread of quinolone resistance. Proper food handling will significantly contribute to decreasing the risk from infection to which people are exposed. In medical and veterinary laboratories antimicrobial resistance monitoring in clinical and environmental isolates is advised. Since correlation between antibiotics application and antimicrobial resistance is often suggested, antimicrobial use must be under strict control of the authorities both in human and in veterinary medicine. .

scholarly journals Evaluasi Penggunaan Silica+® sebagai Feed Additive terhadap Metabolisme Mineral, Status Kesehatan dan Kualitas Ekskreta Broiler

2017 ◽  
Vol 41 (3) ◽  
pp. 285
Author(s):  
Gusma Gama Maradon ◽  
Sumiati Sumiati ◽  
Rita Mutia ◽  
Wiwin Winarsih
Keyword(s):  
Health Status ◽  
Mineral Metabolism ◽  
Feed Additive ◽  
Blood Profile ◽  
E Coli ◽  
Tibia Bone ◽  
Randomized Design ◽  
Zn Content ◽  
High Nutrient

This study aimed to evaluate dietary inclusion of silica+® on mineral metabolism, health status and excreta quality of broilers. This study used a completely randomized design (CRD) with 6 treatments and 6 replications (40 birds of each). The treatments were high nutrient diet (T1), T1 + silica+® 200 ppm (T2), low nutrient diet (T3), T3 + silica+® 200 ppm (T4), feed contain local feedstuff (rice bran) (T5), T5 + silica+® 200 ppm (T6). Parameters measured were minerals consumption, minerals retention, mineral content of the tibia, blood profile (erythrocytes, hemoglobin, hematocrit, leukocytes, differentiation of leukocytes and ratio H/L) and excreta quality (pH, water content and ammonia). The results showed that using silika+® 200 ppm increased (P <0.05) mineral consumption and excretion in low nutrient diet (T4), lowered (P <0.05) retention of Ca and Zn in high nutrient diet (T2), lowered (P<0.05) retention of Zn in low nutrient diet (T2), increased (P<0.05) Ca content in tibia bone in high nutrient diet (T2), increased Ca and Zn content in the tibia (P <0.05) in feed contain ricebran (T6), decreased the amount of fecal NH3 (P <0.05) in feed contain ricebran (T6), lowered E. coli in high nutriet diet (T2) and feed contain ricebran (T6). The conclusion of this study that silica+® could be used as feed additive to increase Ca and Zn deposition in tibia bone, lowering fecal NH3, lowering E. coli in ileum withouth any effect to the health status of broilers.

scholarly journals Serum biochemical properties of Vietnamese indigenous Noi chicken at 56 days old

2020 ◽  
Vol 36 (3) ◽  
pp. 329-339
Author(s):  
Nguyen Giang ◽  
Nguyen Hien ◽  
Huynh Loan ◽  
Phan Phuc ◽  
Nguyen Dai ◽  
...  
Keyword(s):  
Total Protein ◽  
Biochemical Parameters ◽  
Animal Health ◽  
Correlation Coefficients ◽  
Biochemical Properties ◽  
Blood Samples ◽  
Male And Female ◽  
Native Breed ◽  
Serum Biochemical ◽  
The Relationship

Serum profile is generally considered as comprehensive data which directly reflected animal health and their potential resistance to environmental, nutritional and pathological stress. The present study aimed to provide physiological reference values for selected biochemical parameters in Noi chickens, a famous Vietnamese native breed, at 56 days old. The collected blood samples of 355 Noi birds (164 males and 191 females) were used to evaluate biochemical serum profile. Glucose, total protein, albumin, globulin, albumin/globulin ratio, triglycerides, cholesterol, creatinine, and uric acid in the serum were measured. The variation in these values arising from different sexes was also investigated. The results indicated that there were no differences (P>0.05) in the assessed indices between male and female Noi chickens. In overall for the whole group, these values were obtained as 250.86 mg/dL, 7.34 g/dL, 3.40 g/dL, 3.94 g/dL, 1.75, 148.54 mg/dL, 190.86 mg/dL, 0.44 mg/dL, and 1.44 mg/dL, respectively. The relationship among the biochemical parameters showed relatively low coefficients (r=0.79 in maximum). The females obtained higher correlation coefficients between levels of total protein and glucose (r=0.42) as well as between total protein and globulin (r=0.79), compared to the males. It was concluded that the male and female chickens performed similar values of serum indices. The contribution of this study might expand the knowledge on the biochemical profile and improved breeding strategies of Vietnamese indigenous Noi chickens.

scholarly journals Prevalence of feline coronavirus, feline leukemia virus, and feline immunodeficiency virus in client-owned cats in Croatia

2021 ◽  
Vol 8 ◽  
pp. 24-38
Author(s):  
Jelena Raukar
Keyword(s):  
Veterinary Medicine ◽  
Feline Immunodeficiency Virus ◽  
Leukemia Virus ◽  
Feline Leukemia Virus ◽  
Antibody Prevalence ◽  
Blood Samples ◽  
Proviral Dna ◽  
Immunodeficiency Virus ◽  
Feline Coronavirus

This study aimed to determine prevalences for anti-FCoV antibody, FeLV antigen, FeLV proviral DNA, and anti-FIV antibody among client-owned cats from the cities of Zagreb and Varaždin in Croatia. Subjects included 106 client-owned cats tested at the Faculty of Veterinary Medicine, Vienna, Austria. Blood samples were tested with IFA for anti-FCoV antibody and IFA FCoV antibody titeres, with ELISA for FeLV p27 antigen, with PCR for FeLV proviral DNA, and with RIM for anti-FIV antibody. Prevalence of FCoV and FeLV was 41.51% and 6.60%, respectively. A coinfection with FeLV/FCoV and FIV/FCoV prevalence was 7.55% and 5.66%. No cats were coinfected with FIV and FeLV. All three viruses were detected, confirming their presence in Croatia. The seroepidemiological findings demonstrate that both feline retroviruses and feline coronavirus are important feline pathogens in Croatia.

Resistance to the tick Rhipicephalus microplus and Babesia bovis infection levels in beef heifers raised in an endemic area of Sao Paulo state, Brazil

2019 ◽  
Vol 59 (5) ◽  
pp. 938 ◽  
Author(s):  
A. M. Maiorano ◽  
R. Giglioti ◽  
M. C. S. Oliveira ◽  
H. N. Oliveira ◽  
J. N. S. G. Cyrillo ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Intraclass Correlation ◽  
Rhipicephalus Microplus ◽  
Babesia Bovis ◽  
Phenotypic Variance ◽  
Beef Heifers ◽  
Animal Blood ◽  
Blood Samples ◽  
Bos Taurus Indicus ◽  
Tick Resistance

Repeatability coefficients (r) for tick resistance and Babesia bovis infection levels and the correlation (ρ) between these traits were estimated in beef heifers, using artificial infestations and short intervals between measurements. Forty heifers, including 20 Bos taurus taurus (Caracu) and 20 Bos taurus indicus (Nelore) animals, were submitted to three artificial infestations with Rhipicephalus microplus larvae at intervals of 14 days. The number of standard female was counted from the 19th through the 23rd day after each infestation, considering only the left side of each animal. Blood samples were collected on Days 0, 6, 20, 34 and 48 after the first infestation. The number of copies of B. bovis DNA (CN) was estimated from blood samples through the quantitative PCR technique to evaluate the level of infection in the animals. Total tick count (TTC), the percentage of return (PRij) and CN were analysed using the MIXED procedure of the SAS program. The r of the variables were estimated by intraclass correlation between measures of the same animal, with the variance component of the animal being divided by the phenotypic variance (residual + animal), using models with a CS structure matrix. Additionally, ρ among variables were estimated using the CORR procedure. The following results were obtained for Caracu and Nelore animals, respectively: 1.83 ± 0.37 and 0.63 ± 0.40 for TTC, 1.10 ± 0.23 and 0.47 ± 0.23 for PRij, and 2.29 ± 0.64 and 2.32 ± 0.58 for CN. The r was moderate for TTC (0.62) and PRij (0.53) and low for CN (0.10). The ρ between TTC and CN obtained in the same measurement day was not significant in either breed (P &gt; 0.05; –0.07 for Caracu and 0.19 for Nelore). Results showed both breeds were able to develop resistance against B. bovis; however, Nelore exhibited higher tick resistance. The r obtained for CN and the weak association with tick resistance indicate that it is not possible to recommend the use of CN as a trait to predict tick resistance in these two breeds.

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