scholarly journals Antihyperglycemic and Antioxidant Activity of Nanoemulsion Extracts of M. affine D. Don Leaves in Alloxan-Induced Rat

2020 ◽  
Vol 5 (3) ◽  
pp. 167
Author(s):  
Safrida Safrida ◽  
Khairil Khairil ◽  
Fani Fardinita
Keyword(s):  
Antioxidant Activity ◽  
Carboxymethyl Cellulose ◽  
Wistar Rats ◽  
Positive Control ◽  
Negative Control ◽  
Glucose Levels ◽  
Carboxymethyl Cellulose Sodium ◽  
Male Wistar Rats

This study determined the antihyperglycemic and antioxidant activity of nanoemulsion extracts of M. affine leaves in alloxan-induced rats. This research used 24 male Wistar rats around three months old which grouped as normal (untreated), negative control (treated with carboxymethyl cellulose sodium/Na-CMC,), positive control (treated with glibenclamide), and various concentration (30, 60, and 90%) of nanoemulsion extract of M. affine leaves groups. The extract of M. affine leaves had an antioxidant activity with IC50 5.30 ppm, categorized as a very strong antioxidant. Furthermore, the administration of this extract decreased glucose levels in antihyperglycemic rats.  We concluded that M. affine leaves extract potential as antioxidants and be developed as an ingredient for diabetic drugs.

Leaf paste of Telfairia occidentalis favourably modulates deleterious effects associated with exposure to diethylnitrosamine in male Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Solomon E. Owumi ◽  
Jeremiah O. Olugbami ◽  
Andrew O. Akinnifesi ◽  
Oyeronke A. Odunola
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Telfairia Occidentalis ◽  
Male Wistar Rats

Abstract Objectives Diethylnitrosamine (DEN) is found in workplaces, processed meats, tobacco smoke, whiskey, etc. It is capable of forming DNA-adducts. Fluted pumpkin (Telfairia occidentalis [To]) is a medicinal plant, and its herbal preparations have been employed variously in ethnomedicine. Furthermore, it has been reported to possess anti-oxidant, anti-cancer, anti-inflammatory properties. We investigated the possible mitigating effect of the leaf paste of To on DEN-induced deleterious effects in male Wistar rats. Methods Forty-five rats weighing between 100 and 150 g were equally divided into nine groups and treated thus: Group 1 (negative control), Group 2 (0.05 mg/kg carboxymethyl cellulose [CMC] daily), Group 3 (positive control, 25 mg/kg bw DEN administered intraperitoneally thrice per week), Group 4 (25 mg/kg bw quercetin [QUE] daily alone), Groups 5 and 6 (100 and 200 mg/kg bw To daily, respectively), Group 7 (25 mg/kg bw DEN and QUE), Groups 8 and 9 (25 mg/kg bw DEN with 100 and 200 mg/kg bw To, respectively). Blood glucose levels, liver damage biomarkers (aspartate aminotransferase [AST], alanine aminotransferase [ALT] and gamma-glutamyltransferase [γ-GT]), frequency of micronucleated polychromatic erythrocyte (mPCEs), and liver histology were assessed. Results DEN significantly (p<0.05) increased blood glucose levels, activities of ALT, AST and γ-GT, and frequency of mPCEs. Histologically, DEN caused a severe architectural anarchy. However, the intervention groups demonstrated the remarkable protective properties of To by ameliorating the adverse effects caused by DEN. Conclusions Taken together, the leaf paste of To is capable of mitigating DEN-induced hepatotoxicity and clastogenicity in male Wistar rats.

Cocos nucifera L. oil alleviates lead acetate-induced reproductive toxicity in sexually-matured male Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Olugbemi T. Olaniyan ◽  
Olakunle A. Ojewale ◽  
Ayobami Dare ◽  
Olufemi Adebayo ◽  
Joseph E. Enyojo ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Histological Analysis ◽  
Cocos Nucifera ◽  
Reproductive Toxicity ◽  
Positive Control ◽  
Negative Control ◽  
Male Wistar Rats ◽  
Cocos Nucifera L

Abstract Objectives Lead primarily affects male reproductive functions via hormonal imbalance and morphological damage to the testicular tissue with significant alteration in sperm profile and oxidative markers. Though, different studies have reported that Cocos nucifera L. oil has a wide range of biological effects, this study aimed at investigating the effect of Cocos nucifera L. oil on lead acetate-induced reproductive toxicity in male Wistar rats. Methods Twenty (20) sexually matured male Wistar rats (55–65 days) were randomly distributed into four groups (n=5). Group I (negative control)—distilled water orally for 56 days, Group II (positive control)—5 mg/kg bwt lead acetate intraperitoneally (i.p.) for 14 days, Group III—6.7 mL/kg bwt Cocos nucifera L. oil orally for 56 days and Group IV—lead acetate intraperitoneally (i.p.) for 14 days and Cocos nucifera L. oil for orally for 56 days. Rats were sacrificed by diethyl ether, after which the serum, testis and epididymis were collected and used for semen analysis, biochemical and histological analysis. Results The lead acetate significantly increases (p<0.05) testicular and epididymal malondialdehyde (MDA) levels, while a significant reduction (p<0.05) in sperm parameters, organ weight, testosterone and luteinizing hormone was observed when compared with the negative control. The coadministration of Cocos nucifera oil with lead acetate significantly increases (p<0.05) testosterone, luteinizing hormone, sperm parameters and organ weight, with a significant decrease (p<0.05) in MDA levels compared with positive control. Histological analysis showed that lead acetate distorts testicular cytoarchitecture and germ cell integrity while this was normalized in the cotreated group. Conclusions Cocos nucifera oil attenuates the deleterious effects of lead acetate in male Wistar rats, which could be attributed to its polyphenol content and antioxidant properties.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals EFEK ANTIDIABETES EKSTRAK AIR KULIT BUAH PISANG AMBON (Musa paradisiaca L.) TERHADAP MENCIT (Mus musculus) MODEL HIPERGLIKEMIA

2015 ◽  
Vol 1 (2) ◽  
pp. 133-140
Author(s):  
Sri Indrawati ◽  
Yuliet Yuliet ◽  
Ihwan Ihwan
Keyword(s):  
Antioxidant Activity ◽  
Effective Dose ◽  
Aqueous Extract ◽  
Positive Control ◽  
Negative Control ◽  
Antidiabetic Activity ◽  
Glucose Levels ◽  
Musa Paradisiaca ◽  
Blood Glucose Levels ◽  
Before And After

Pisang Ambon (Musa paradisiaca L.) is one type of bananas usually consumed by Indonesian people. Besides its flesh which has high nutrition, its peels also has antioxidant activity. Antioxidants has the ability to reduce oxidative damage in people’s body with diabetes mellitus. Therefore, this study aimed to determine the antioxidant activity of the aqueous  extract of Pisang Ambon peels and to determine it’s effective dose as an antidiabetic agent in hyperglycemic mice. This study used male mice which all have been intravenously induced with alloxan at a dose of 50 mg/kgBW. They were then divided into five groups. The first two groups got Na CMC 0.5% (negative control) and glibenclamide 0.65 mg/kgBW (positive control), while the other three got  the aqueous  extract of Pisang Ambon peels successively at doses of 400, 800, and 1200 mg/kgBW. The data were statistically analyzed using ANOVA (Analysis of Variance) at 95% confidence interval with parameter of blood glucose levels difference between before and after treatment. The results showed that the aqueous extract of Pisang Ambon peels had antidiabetic activity at an effective dose of 400 mg/kgBW in hyperglycemic mice which was comparable to glibenclamide

scholarly journals Antimalarial combination therapies increase gastric ulcers through an imbalance of basic antioxidative-oxidative enzymes in male Wistar rats

2020 ◽  
Author(s):  
Muhamudu Kalange ◽  
Miriam Nansunga ◽  
Keneth Iceland Kasozi ◽  
Josephine Kasolo ◽  
Jackline Namuleme ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Wistar Rats ◽  
Saline Group ◽  
Positive Control ◽  
Negative Control ◽  
Oxidative Enzymes ◽  
Gastric Ulcers ◽  
Combination Therapies ◽  
Male Wistar Rats ◽  
High Gastric Ulcer

Abstract Objective: Antimalarials are globally used against plasmodium infections, however, information on the safety of new antimalarial combination therapies on the gastric mucosa is scarce. The aim of the study was to establish the effects of Artesunate-Amodiaquine and Artemether-Lumefantrine on gastric ulcers, malondialdehyde (MDA), reduced glutathione (GSH) and identify major histological changes in male Wistar rats. Gastric ulcers were induced using Indomethacin in four groups and group 1 was administered Artesunate, group 2 received Artesunate-Amodiaquine, group 3 received Artemether-Lumefantrine, and group 4 was a positive control (normal saline). Group five was the negative control consisting of healthy rats. Results: Antimalarial combination therapies were associated with a high gastric ulcer index than a single antimalarial agent, Artesunate. In addition, levels of MDA were significantly higher in the combination of therapies while levels of GSH were lower in comparison to Artesunate and the negative control. Microscopically, antimalarial combination therapies were associated with severe inflammation and tissue damage than Artesunate in the gastric mucosa showing that antimalarial combination therapies exert their toxic effects through oxidative stress mechanisms, and this leads to apoptosis. Findings in this study demonstrate a new to revisit information on the pharmacodynamics of major circulating antimalarial agents in developing countries.

scholarly journals Calcium and s100a1 protein balance in the brain–heart axis in diabetic male Wistar rats

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Keneth Iceland Kasozi ◽  
Dorothy Nakimbugwe ◽  
Herbert Izo Ninsiima ◽  
Josephine Kasolo ◽  
Kevin Matama ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Feed Supplementation ◽  
Grain Amaranth ◽  
Amaranthus Hypochondriacus ◽  
Group I ◽  
Male Wistar Rats ◽  
The Brain

AbstractObjectivesCalcium deregulation in diabetes mellitus (DM) is central to the brain–heart axis pathology. This has led to the use of medical plants in complementary medicine such as Amaranthus hypochondriacus (GA). The objective of the study was to establish the effects of grain amaranth feed supplementation on calcium, s100al protein and antioxidant levels on the brain–heart axis in diabetic male Wistar rats.MethodsThe study involved six groups (n=5) with DM being induced in 20 rats. To the diabetic rats, Group I received mixtard®, Group II was positive control, Groups III and IV received GA feed supplementation at 25 and 50%. In the nondiabetic rats (n=10), Group V received 50% grain amaranth while Group VI was the negative control. The brain and heart tissues were harvested after five weeks and processed using standard methods.ResultsGrain amaranth feed supplementation led to improved calcium levels in DM as compared to the positive control. This also led to increased s100a1, antioxidant levels in the brain–heart axis during DM. This then protected the tissues against oxidative damage, thus preserving tissue function and structure.ConclusionsGrain amaranth’s actions on calcium signaling subsequently affected s100a1 protein levels, leading to improved tissue function in diabetes.

scholarly journals Evaluation of the effect of different concentrations of Arsenicum album 6cH on intoxicated rats

2021 ◽  
Vol 8 (28) ◽  
pp. 119-127
Author(s):  
Olney Leite Fontes ◽  
Fátima Cristiane Lopes Goularte Farhat ◽  
Amarilys Toledo Cesar ◽  
Marilisa Guimarães Lara ◽  
Maria Imaculada Lima Montebelo ◽  
...  
Keyword(s):  
Atomic Absorption Spectroscopy ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Groups ◽  
Before And After ◽  
Male Wistar Rats

Aims: Homeopaths diverge on the concept of dose, i.e. the amount of drug that a patient must take to alter his or her state of disease. In order to stimulate reflections on this concept, this study sought to evaluate in vivo the effect of different concentrations of Arsenicum album 6cH prepared according to homeopathic pharmacotechnics. Methods: male Wistar rats were intoxicated with arsenic and then treated with Arsenicum album 6cH and Arsenicum album 6cH diluted at 1%, administered orally. The amount of arsenic retained in the animals’ organism and that eliminated by urine were measured through atomic absorption spectroscopy. Samples of urine were collected before and after intoxication and during treatment. The positive control group (intoxicated animals) and the negative control group (non-intoxicated animals) received only the vehicle used in the preparation of the medicine. Results: Groups treated with Arsenicum album 6cH and Arsenicum album 6cH diluted at 1% eliminated significant amounts of arsenic when compared to the control groups. The group treated with Arsenicum album 6cH eliminated significantly higher amounts of arsenic than the group treated with the diluted medicine at 1%. Conclusion: results suggest that Arsenicum album 6cH should not be diluted as not to compromise its effectiveness in the treatment of rats intoxicated with arsenic.

scholarly journals Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit

2017 ◽  
Vol 9 (3) ◽  
pp. 312-317
Author(s):  
KARTIAWATI ALIPIN ◽  
EMA PURNAMA SARI ◽  
MADIHAH MADIHAH ◽  
TIA SETIAWATI ◽  
NINING RATNINGSIH ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
Positive Control ◽  
Negative Control ◽  
Kidney Damage ◽  
Diabetic Rat ◽  
Control Group ◽  
Male Wistar Rats

Alipin K, Sari EP, Madihah, Setiawati T, Ratningsih N, Malini DM. 2017. Kidney histology in streptozotocin-induced diabetic male Wistar rats treated with combined extract of temulawak rhizome and belimbing wuluh fruit. Nusantara Bioscience 9: 312-317. Complications that are occurred in patients with Diabetes Mellitus usually followed by kidney damage. Temulawak (Curcuma xanthorrhiza Roxb.) and belimbing wuluh (Averrhoa bilimbi L.) were traditionally used to decrease blood glucose level. Thus, they were potential as antidiabetic drugs. This study aimed to evaluate the combination of ethanol extracts of temulawak rhizome and belimbing wuluh fruit in repairing kidney damage in diabetic male Wistar rats induced by streptozotocin (STZ). An experimental method using a completely randomized design that consist of seven treatments with three replications. Six treatment groups were injected intraperitoneally with a dose of 60 mg/kg BW STZ, and one group served as a control. The animals which have blood glucose level ≥200 mg/dl were stated as diabetic. Furthermore, the animals were treated orally with single extract i.e. temulawak 17.5 mg/kg BW or belimbing wuluh 750 mg/kg BW and combined extracts 383.75 or 767.5 mg/kg BW, as well as glibenclamide 0.45 mg/kg BW as reference, including diabetic rat as positive control and non-diabetic rat as negative control. The results showed that combine extract at dose of 383.75 mg/kg BW treatment repaired the kidney histology, i.e., glomerular diameter and Bowman space width, as well as significantly decreased the necrosis percentage of proximal tubular in diabetic rat compared with positive control group (p<0.05). In conclusion, the combined extract of temulawak rhizome and belimbing wuluh fruit has potent to cure renal failure in diabetic rats induced by streptozotocin.

scholarly journals ANTIHIPERGLIKEMIK EKSTRAK TUMBUHAN SURUHAN (Peperomia pellucida [L.] Kunth) TERHADAP TIKUS WISTAR (Rattus norvegicus L.) YANG DIINDUKSI SUKROSA

2013 ◽  
Vol 13 (2) ◽  
pp. 116 ◽  
Author(s):  
Nafila Salma ◽  
Jessy Paendong ◽  
Lidya I Momuat ◽  
Sariyana Togubu
Keyword(s):  
Blood Glucose ◽  
Treatment Group ◽  
Blood Sugar ◽  
Plant Extract ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Negative Control ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Male Wistar Rats

ANTIHIPERGLIKEMIK EKSTRAK TUMBUHAN SURUHAN (Peperomia pellucida [L.] Kunth) TERHADAP TIKUS WISTAR (Rattus norvegicus L.) YANG DIINDUKSI SUKROSAABSTRAK Telah dilakukan penelitian untuk menentukan dosis optimum dari ekstrak tumbuhan suruhan (Peperomia pellucida [L.] Kunth) sebagai antihiperglikemik tikus wistar yang diinduksi sukrosa. Penelitian ini menggunakan metode uji toleransi glukosa. Digunakan tikus putih jantan wistar berjumlah 20 ekor tikus yang dibagi dalam 5 kelompok perlakuan yaitu kelompok perlakuan kontrol negatif (K-) diberi CMC 0,5%, kelompok perlakuan kontrol positif (K+) diberi Glibenklamid dosis 0,45 mg/kgBB, kelompok perlakuan ekstrak tumbuhan suruhan  dosis 20; 40; 80 mg/kgBB. Data diperoleh dari pemeriksaan kadar gula darah puasa (basal), 30 menit setelah diindukasi sukrosa dan pada menit ke 30, 60, dan 120 setelah diinduksi sediaan per oral. Data diolah dengan uji ANOVA dan Ducan untuk membandingkan antara kelompok kontrol negatif, ekstrak dan kontrol positif. Data hasil pengujian kadar glukosa darah dengan dosis 20; 40; 80 mg/kgBB memberikan penurunan kadar glukosa darah. Pemberian ekstrak tumbuhan suruhan dengan dosis 40 mg/kgBB pada menit ke-120 memberikan hasil  yang signifikan dan bebeda nyata dibandingkan kelompok kontrol negatif (CMC 0,5%) dan tidak berbeda nyata dengan glibenklamid dosis 0,45 mg/kgBB. Kesimpulannya pemberian ekstrak tumbuhan suruhan dosis 40 mg/kgBB pada menit ke 120  memberikan penurunan kadar glukosa darah yang efektif dan berbeda nyata (signifikan) dibandingkan pemberian ekstrak tumbuhan suruhan dosis 20 dan 80 mg/kgBB. Kata Kunci : Peperomia pellucida [L.] Kunth, Tumbuhan Suruhan, Kadar Glukosa Darah, Sukrosa. ANTIHYPERGLYCEMIC FROM SURUHAN PLANT (Peperomia pellucida [L.] Kunth) EXTRACT AGAINST WISTAR RATS (Rattus norvegicus L.) WITH INCLUDED BY SUCROSE ABSTRACT The purpose of this research is to determine optimum dose of Suruhan (Peperomia pellucida [L.] Kunth) extract as Antihyperglycemic agent against Wistar Rats with Induced by Sucrose. This research using glucose tolerance test methods. Used 20 male Wistar rats were divided into 5 groups, they are the negative control treatment group (K-) were given 0.5% CMC, the positive control groups (K+) given 0,45 mg/kgBB  Glibenklamid, and Suruhan extract treatment group of 20; 40; 80 mg/kgBB dose. Data obtained from the examination offasting blood sugar (basal) content, 30 minutes after induced bysucroseandat minute of 30, 60, and120 after-induced oraldosage.The data were processed by ANAVA and LSD testtocompare betweenthe negativecontrol group, the extractandpositive control. Dataresults of testing blood sugar levels with doses of 20, 40, 80 mg/kgBB gavea decreasein blood glucose levels were significant and significantly different than the negative control group (CMC 0.5%) and did not give a clear difference with 0.45 mg/kgBB dose of Glibenclamide. Agents of plant extract at a dose of 40 mg/kgBB body weight gives better results to the decline in blood glucose levels compared with doses of 20 and 80 mg/kgBB. In conclusion, Suruhan plant extract has an effect on blood glucose levels decreased against white male Wistar rats were induced sucrose. Keywords : Peperomia pellucida [L.] Kunth, Suruhan Plant, Blood Glucose Levels, Sucrose

scholarly journals Ingredients of Active Compounds and Anti-Diabetic Test of Water Extract of Lannea coromandelica (Houtt) Merr. On Wistar Rats

2021 ◽  
pp. 116-122
Author(s):  
Nona Rahmaida Puetri ◽  
Rita Marleta Dewi ◽  
Sahrul ◽  
Yasir ◽  
Munawir Annasri
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Descriptive Analysis ◽  
Water Extract ◽  
Positive Control ◽  
Negative Control ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Lannea Coromandelica ◽  
Sugar Levels

Leaves of Lannea coromandelica (Houtt.) Merr. is one of the plants found in Aceh. This study aims to determine the effect of giving water extract of Lannea coromandellica (Houtt.) Merr. against STZ-induced blood sugar levels of wistar rats. The experimental study used a sample of 25 male and 15 female Wistar rats. This research is experimental with a sample of 25 male and 15 female of Wistar rats. The treatments were given, namely negative control (aquades), positive control (metformin), 50 mg EALC, 100 mg EALC, and 150 mg EALC. The data obtained will be analyzed by using the Kruskal Wallis test to obtain the effectiveness of the treatment and also a descriptive analysis for the average reduction in blood glucose levels. Macro and histological observations were also carried out on the liver and pancreas. The results obtained from the Kruskal Wallis test results showed that there was no effect of EALC on changes in the decrease in blood glucose levels of wistar rats for 14 days of treatment. Observation of the macro organs showed pancreas had a changes. While the histological observations pancreas and liver showed changes. The results of this study indicate that the effectiveness of EALC is not proven to have an effect, this is presumably because the extract concentration is too small so it is not enough to work optimally. Phytochemical test shows that EALC contains flavonoids, steroids, phenolics, tannins, saponins and alkaloids.

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