scholarly journals Ingredients of Active Compounds and Anti-Diabetic Test of Water Extract of Lannea coromandelica (Houtt) Merr. On Wistar Rats

2021 ◽  
pp. 116-122
Author(s):  
Nona Rahmaida Puetri ◽  
Rita Marleta Dewi ◽  
Sahrul ◽  
Yasir ◽  
Munawir Annasri
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Descriptive Analysis ◽  
Water Extract ◽  
Positive Control ◽  
Negative Control ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Lannea Coromandelica ◽  
Sugar Levels

Leaves of Lannea coromandelica (Houtt.) Merr. is one of the plants found in Aceh. This study aims to determine the effect of giving water extract of Lannea coromandellica (Houtt.) Merr. against STZ-induced blood sugar levels of wistar rats. The experimental study used a sample of 25 male and 15 female Wistar rats. This research is experimental with a sample of 25 male and 15 female of Wistar rats. The treatments were given, namely negative control (aquades), positive control (metformin), 50 mg EALC, 100 mg EALC, and 150 mg EALC. The data obtained will be analyzed by using the Kruskal Wallis test to obtain the effectiveness of the treatment and also a descriptive analysis for the average reduction in blood glucose levels. Macro and histological observations were also carried out on the liver and pancreas. The results obtained from the Kruskal Wallis test results showed that there was no effect of EALC on changes in the decrease in blood glucose levels of wistar rats for 14 days of treatment. Observation of the macro organs showed pancreas had a changes. While the histological observations pancreas and liver showed changes. The results of this study indicate that the effectiveness of EALC is not proven to have an effect, this is presumably because the extract concentration is too small so it is not enough to work optimally. Phytochemical test shows that EALC contains flavonoids, steroids, phenolics, tannins, saponins and alkaloids.

scholarly journals UJI AKTIVITAS ANTIDIABETES KOMBINASI EKSTRAK ETANOL DAUN ECENG GONDOK (Eichhornia crassipes (Mart.)Solms) DAN DAUN SINTRONG (Crassocephalum crepidioides (Benth.) S. Moore) PADA MENCIT

2021 ◽  
Vol 4 (1) ◽  
pp. 44-53
Author(s):  
Andri Priyoherianto ◽  
◽  
Panji Ratih Suci ◽  
Putri Rizki Cahya Fatimah ◽  
Atik Nur Wijayanti
Keyword(s):  
Blood Glucose ◽  
Blood Sugar ◽  
Water Hyacinth ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Glucose Levels ◽  
Normal Limits ◽  
Blood Glucose Levels ◽  
Sugar Levels

Diabetes mellitus is a chronic metabolic disorder that occurs due to destruction of pancreatic ? cells characterized by blood glucose levels that exceed normal limits. The purpose of this study was to determine the therapeutic effect of the combination of water hyacinth leaf extract and Sintrong leaf in reducing blood glucose levels in alloxan-induced experimental animals. Water hyacinth leaf powder and Sintrong leaves were extracted using the maceration method with 70% ethanol as a solvent. A total of 25 mice were divided into 5 groups, namely negative control CMC-Na 0.5%, positive control glibenclamide 0.00065 mg / g BW, water hyacinth leaf extract 17.5 mg / kg BW, Sintrong leaf extract 10.5 mg/ kg BW and a combination of water hyacinth leaf extract 17.5 mg / kg BW and sintrong leaf 10.5 mg / kg BW. The results showed that a single extract of water hyacinth and Sintrong leaves was able to reduce blood sugar levels for 7 days. The combination dose was effective in reducing blood sugar levels with a combination dose of 17.5 mg/ kg water hyacinth leaf extract in combination for 7 days.

Leaf paste of Telfairia occidentalis favourably modulates deleterious effects associated with exposure to diethylnitrosamine in male Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Solomon E. Owumi ◽  
Jeremiah O. Olugbami ◽  
Andrew O. Akinnifesi ◽  
Oyeronke A. Odunola
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Telfairia Occidentalis ◽  
Male Wistar Rats

Abstract Objectives Diethylnitrosamine (DEN) is found in workplaces, processed meats, tobacco smoke, whiskey, etc. It is capable of forming DNA-adducts. Fluted pumpkin (Telfairia occidentalis [To]) is a medicinal plant, and its herbal preparations have been employed variously in ethnomedicine. Furthermore, it has been reported to possess anti-oxidant, anti-cancer, anti-inflammatory properties. We investigated the possible mitigating effect of the leaf paste of To on DEN-induced deleterious effects in male Wistar rats. Methods Forty-five rats weighing between 100 and 150 g were equally divided into nine groups and treated thus: Group 1 (negative control), Group 2 (0.05 mg/kg carboxymethyl cellulose [CMC] daily), Group 3 (positive control, 25 mg/kg bw DEN administered intraperitoneally thrice per week), Group 4 (25 mg/kg bw quercetin [QUE] daily alone), Groups 5 and 6 (100 and 200 mg/kg bw To daily, respectively), Group 7 (25 mg/kg bw DEN and QUE), Groups 8 and 9 (25 mg/kg bw DEN with 100 and 200 mg/kg bw To, respectively). Blood glucose levels, liver damage biomarkers (aspartate aminotransferase [AST], alanine aminotransferase [ALT] and gamma-glutamyltransferase [γ-GT]), frequency of micronucleated polychromatic erythrocyte (mPCEs), and liver histology were assessed. Results DEN significantly (p<0.05) increased blood glucose levels, activities of ALT, AST and γ-GT, and frequency of mPCEs. Histologically, DEN caused a severe architectural anarchy. However, the intervention groups demonstrated the remarkable protective properties of To by ameliorating the adverse effects caused by DEN. Conclusions Taken together, the leaf paste of To is capable of mitigating DEN-induced hepatotoxicity and clastogenicity in male Wistar rats.

scholarly journals Ekstrak Daun Asam Jawa (Tamarindus Indica L.) Sebagai Antidiabetes

2017 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
Olfiana T. Lahamado ◽  
Sri Mulyani Sabang ◽  
Kasmudin Mustapa
Keyword(s):  
Blood Glucose ◽  
Positive Control ◽  
Negative Control ◽  
Tamarindus Indica ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Significant Difference ◽  
Infusion Method ◽  
Ethylene Diamine Tetra Acetate ◽  
Sugar Levels

One of the plant that is widely used as a medicinal plant is the leaves of tamarind (tamarindus indica L). This study aims to determine the effectiveness of anti-diabetic of tamarind (tamarindus indica L.) extract in lowering blood glucose levels in mice animals test. The experiment was performed using tamarind leaves extracted through infusion method. The animals test were 15 male mice induced by ethylene diamine tetra acetate (EDTA). Mice were divided randomly into 5 groups with different treatments. Treatments I, II and III were given tamarind leaves extracts for each with a concentration of 10%, 20% and 40%. Treatment IV was given glibenclamide suspense as a positive control (+), and the treatment V was given Na-CMC 1% as a negative control (-). Based on the analysis of variance statistical calculations tamarind leaves extracts can lower blood glucose levels on mice test. Furthermore, the significant difference was tested to check the concentration effect. The results on animals test with a significant level of 5% showed that the tamarind leaves extract of 40% or Treatment III affected on decreasing blood sugar levels on mice.

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

scholarly journals Effectiveness of Graptophyllum pictum (L.) Griff leaf extraction on blood glucose level in alloxan-induced Wistar rat

Food Research ◽  
2020 ◽  
Vol 4 (S3) ◽  
pp. 123-126
Author(s):  
A. Leonoreza ◽  
T. Excelinda ◽  
J. Elnitiarta ◽  
H.S. Heri-Nugroho ◽  
M. Hendrianingtyas ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Treatment Group ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Negative Control ◽  
Normal Group ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Blood Glucose Levels

Diabetes mellitus is a metabolic disease that always increasing in prevalence. Excessive glucose intake and oxidative stress that can lead to damage to the pancreatic cell or insulin resistance, are such factors that influence the occurrence of diabetes mellitus. Graptophyllum pictum (L.) Griff leaf contains active metabolites such as flavonoid which able to decrease free radical levels. This study aimed to prove the effectiveness of Graptophyllum pictum (L.) Griff leaves extract on blood glucose levels in alloxan-induced Wistar rats. In this study, twenty-five male Wistar rats were divided into five groups. On day 0, the negative control and each treatment group injected with 125 mg/kg dose of alloxan and would be checked on 4th day. On the 4th day after the rats had diabetes, normal group (K-1) was given only standard feed, Negative control (K-2) was given only alloxan intraperitoneally on day 0, first treatment group (K-3) was given 50 mg/kg dose of Graptophyllum pictum (L.) Griff, second treatment group (K-4) was given 100 mg/kg dose of the leaf extract, and the third treatment group (K-5) was given the leaf 200 mg/kg dose of the leaf extract. On the 10th day, fasting blood glucose was taken from the rats. Post-test only group design was used to be the experimental design. There were significant differences in the treatment groups (K-3, K-4, and K-5) compared to the negative control group (K-2) after treatment. The mean of blood glucose levels decreased with the increased doses of Graptophyllum pictum (L.) Griff leaf extract treatment. The treatment groups (K-3, K-4, and K-5) were not significant compared to the normal group (K-1).

scholarly journals THE EFFECTS OF CORN FLOUR WITH TEMPEH FLOUR SUPPLEMENTATION FEEDING IN DIABETES MELLITUS WISTAR RATS TOWARD BLOOD GLUCOSE LEVEL

2017 ◽  
Vol 4 (2) ◽  
pp. 24
Author(s):  
Fatifa Asmarani ◽  
Bambang Wirjatmadi ◽  
Merryana Adriani
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Blood Sugar ◽  
Wistar Rats ◽  
Control Group ◽  
Standard Diet ◽  
Corn Flour ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Sugar Levels

Background: Diabetes mellitus (DM) is metabolic disorder syndrome characterized by hyperglycemia due to deficiency of insulin secretion. Patients of diabetic patients increases each year, so it needs proper handling. Corn flour is a source of carbohydrates with a low glycemic index to help reduce the rise in blood sugar levels. In addition, supplementation with tempeh flour containing isoflavones act to protect cells from free radical thereby inhibiting damage cells. Fiber in tempeh affect blood glucose levels because it slows the absorption of glucose.Objective: To determine the effect supplementation of corn flour with tempeh flour on blood sugar levels in diabetes mellitus Wistar rats.Methods: The study was true experimental design with Pre-posttest control group design, samples were 20 male Wistar rats aged 2-3 months were then divided into 4 groups (T0, T1, T2, T3.) T0 was injected with alloxan as 30 mg / 150 grams of BB rats are subsequently treated with standard diet; T1 was dministered by standard diet 50% + 50% corn flour; T2 was administered by standard diet 50% + 30% + corn flour tempeh flour 20%; and T3 was administered by standard diet of corn flour 50% + 15% + 35% tempeh flour for 2 weeks. Blood glucose levels were measured with a glucose kit (GOD FS). Research data were analyzed by One Way ANOVA followed by Tukey's Post Hoc test p-value <0.05.Results: There were significant differences on blood sugar levels in all groups (p = 0.000): control group (only dietary standards), T1 (administered by standard diet 50% + corn flour 50%), T2 (administered by a standard diet 50% + cornstarch 30% tempeh flour + 20%) and T3 (administered by standard diet of corn flour 50% + 15% + 35% tempeh flour).Conclusion: corn flour with tempeh flour supplementation effect on decresing of blood sugar levels.

scholarly journals In Vivo Activities and In Silico Study of Jackfruit Seeds (Artocarpus heterophyllus Lam.) on the Reduction of Blood Sugar Levels of Gestational Diabetes Rate Induced by Streptozotocin

2019 ◽  
Vol 7 (22) ◽  
pp. 3819-3826
Author(s):  
Dwitiyanti Dwitiyanti ◽  
Rizky Arcinthya Rachmania ◽  
Kriana Efendi ◽  
Riri Septiani ◽  
Pahlevi Jihadudin
Keyword(s):  
Molecular Docking ◽  
Blood Glucose ◽  
Virtual Screening ◽  
Blood Sugar ◽  
Positive Control ◽  
Artocarpus Heterophyllus ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
In Silico Study ◽  
Sugar Levels

BACKGROUND: Jackfruit Seeds (Artocarpus heterophyllus L.) are plants that are thought to be able to reduce blood sugar levels. AIM: The purpose of this study was to prove the activity of jackfruit seeds in reducing blood glucose levels in gestational diabetic rats induced by streptozotocin and in silico study virtual screening through molecular docking to find out the compounds in jackfruit seeds that have interaction with sulfonylurea receptors. METHODS: The animals used in this study were Sprague Dawley strain pregnant female rats which were divided into six groups, namely the normal control group, negative control, positive control, various dose groups (100 mg/kg BW, 200 mg/kg BW, 400 mg/kg BW). After the rat was pregnant, the rats were induced first with streptozotocin so that the rats had hyperglycemia. Blood glucose levels were measured on the 14th day after treatment. The data obtained were statistically tested by one-way ANOVA test followed by the HSD Tukey test. Virtual screening was done using PLANTS 1.2 software. RESULTS: The results showed that all groups of ethanol extract 70% of jackfruit seeds could reduce blood glucose levels. The biggest decrease in blood glucose levels occurred at dose 3 with a dose of 400 mg/kg BW which was 61.73%, comparable to positive control glibenclamide. The results of virtual screening with molecular docking showed that betacarotene epoxide compounds have better affinity than glibenclamide as a comparative compound. CONCLUSION: It can be concluded that jackfruit seeds beta-carotene epoxide has the potential to reduce blood sugar levels by inducing insulin secretion.

scholarly journals Uji efek ekstrak buah okra (Abelmoschus esculentus) terhadap kadar glukosa darah pada tikus wistar (Rattus norvegicus) yang diinduksi aloksan

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Leonardus B.A. Prakoso ◽  
Christi Mambo ◽  
Mona P. Wowor
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Negative Control ◽  
Abelmoschus Esculentus ◽  
Control Group ◽  
Elevated Blood ◽  
Glucose Levels ◽  
Elevated Blood Glucose ◽  
Blood Glucose Levels

Abstract: Okra (Abelmoschus esculentus) is one of many herbal medicine that have been used worldwide to prevent and treat diabetes mellitus. This research aimed to evaluate the effect of garlic bulbs extract administration on elevated blood glucose levels on Wistar rats (R. norvegicus) induced by alloxan. The subject in this research were male Wistar rats with total of 15 samples divided into 5 groups, consisting of one negative control group, one positive control group who were induce by 130 mg/kgBW of alloxan that cause the Rats in conditions of hyperglycemic, and three groups of Rats were given okra extract with dose of 0.375 ml/100 gBW of rat, 0.75 ml/100 gBW of rat , and 1.5 ml/100 gBW of rat. Blood glucose levels was measured on day zero, day one and day two every thirty minutes on 0, 30, 60, 90, 120, and every six hours on 0, 6, 12, 18, and 24. Data from the measurement showed that 0.375 ml/100 gBW, 0.75 ml/100 gBW , and 1.5 ml/100 gBW of okra extract can’t reduce elevated blood glucose levels on Wistar rats.Keywords: Abelmoschus esculentus, okra, blood glucose levels, alloxan Abstrak: Buah Okra (Abelmoschus esculentus) diyakini masyarakat sebagai salah satu tanaman obat yang mampu mencegah dan mengobati penyakit diabetes. Penelitian ini bertujuan untuk menguji apakah pemberian ekstrak buah okra dapat menurunkan kadar glukosa darah tikus Wistar (R. norvegicus) yang diinduksi aloksan. Subjek penelitian berupa tikus Wistar berjumlah 15 ekor yang dibagi dalam 5 kelompok, terdiri atas satu kelompok kontrol negatif, satu kelompok kontrol positif yang diberi aloksan dengan dosis 130 mg/kgBB yang menyebabkan tikus Wistar dalam keadaan hiperglikemik yang kemudian diinsuksi insulin, dan tiga kelompok Tikus hiperglikemik diberi ekstrak buah okra dengan dosis 0,375 ml/100 gBB tikus, 0,75 ml/100 gBB tikus, dan 1,5 ml/100 gBB tikus. Data diperoleh dari pemeriksaan kadar glukosa darah pada semua kelompok tikus Wistar pada hari ke nol, pertama, dan kedua pada menit ke-0, 30, 60, 90, 120 dan jam ke-0, 6, 12, 18, dan 24. Hasil penelitian menunjukkan bahwa pemberian ekstrak buah okra dengan dosis 0,375 ml/100 gBB tikus, 0,75 ml/100 gBB tikus, dan 1,5 ml/100 gBB tikus tidak mempunyai efek menurunkan kadar glukosa darah tikus Wistar. Kata kunci: abelmoschus esculentus, buah okra, kadar glukosa darah, aloksan

scholarly journals The Effectiveness of Combination of Momordica charantia Extract and Averrhoa bilimbi on Nf-Kb Activation in Mice (Mus musculus) Balb/C Hyperglycemia Models

2020 ◽  
Vol 10 (1) ◽  
pp. 61-64
Author(s):  
Intan Sartika Risky Sampurna ◽  
◽  
Muhaimin Rifa’i ◽  
Sri Rahayu
Keyword(s):  
Blood Glucose ◽  
Medical Condition ◽  
Positive Control ◽  
Momordica Charantia ◽  
Control Treatment ◽  
Cd8 Cells ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Sugar Levels ◽  
System Disorder

Hyperglycemia is a medical condition when the blood glucose levels increase exceeds the reasonable limit. Hyperglycemia is a typical sign of diabetes mellitus (DM). Indonesia is the sixth-ranked country in the world after China, India, United States, Brazil, and Mexico. Treatment with herbs is currently being developed. Pare (Momordica charantia) and Belimbing wuluh (Averrhoa bilimbi) are plants that found around us. Some studies state that each of these plants can be anti-diabetic. The hyperglycemia can cause an immune system disorder characterized by pancreatic β cell death involving IL-1β, kappa B (NF)-κB nuclear factor, and Fas. The ability of NF-kB activation will affect the number of cytokines expressed by T cells, namely TNF-α, and IFN-γ. The purpose of this study is to determine the effect of NF-kB activation on blood glucose levels in hyperglycemia mice. The results showed that the positive control treatment showed an increase in the number of NF-kB activations in CD4 and CD8 cells. EPBW (combination of Averrhoa bilimbi extract and Momordica charantia) administration results at a dose of 10 mg.kg-1 BW showed a reduction in the amount of activated NF-kB as a substitute for the reduction. In addition, that dose can reduce blood sugar levels in mice hyperglycemia model.

scholarly journals The Analysis of the Chlorogenic Acid in the Ethanol Fraction of Robusta Coffee Beans and Its Effect on Glucose Levels in Wistar Rats

2021 ◽  
Vol 15 (2) ◽  
pp. 118
Author(s):  
Rusman Hasanuddin ◽  
Jasmiadi Jasmiadi ◽  
Nurliana Abdillah
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Chlorogenic Acid ◽  
Wistar Rats ◽  
Cell Damage ◽  
Negative Control ◽  
Glucose Levels ◽  
Robusta Coffee ◽  
Blood Glucose Levels ◽  
Ethanol Fraction

Background: The metabolic disorder caused by high blood glucose levels and pancreatic beta-cell damage is known as diabetes mellitus. Indonesia itself is the 7th country in the world with the number of people with diabetes mellitus. Indonesia occupies rank 7 in the world with the number of people with diabetes mellitus. Apart from that, robusta coffee (Coffea canephora L) is one of the most popular drinks globally, including Indonesia. Chlorogenic acid in coffee beans effectively reduces cell damage due to free radicals, including minimizing excessive glucose release from the liver into the blood. Objective: This study aimed to analyze the effects of chlorogenic acid in the ethanolic fraction of robusta coffee (Coffea canephora L) beans on blood glucose levels in Wistar rats. Method: The researchers applied an experimental study with a randomized pretest-posttest control group design. The beans of robusta coffee were extracted using the Maceration method and then fractionated using a hexane and ethyl acetate solvent. The concentration of the obtained remaining fraction was measured using a spectrophotometer. Furthermore, hyperglycemia testing included 30 Wistar rats induced with 20% glucose for 3 – 4 weeks. They were then given the ethanol fraction of robusta coffee with a dose of 400 mg/kg BW and 500 mg/kg BW. Meanwhile, metformin served as a positive control, and NaCMC served as a negative control. Results: The chlorogenic acid analysis in the ethanol fraction of robusta coffee on a spectrophotometer with a concentration of 37% indicated a decrease of 16.66% on the negative control and 48.06% on the positive control. Meanwhile, the ethanol fraction of each control was 51.53% and 52.16%, respectively. Conclusion: The ethanol fraction of the robusta coffee significantly affects the decrease in blood glucose levels in Wistar rats.

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