scholarly journals EVALUATION OF TRACHYSPERMUM AMMI SEEDS FOR ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS

2018 ◽  
Vol 11 (5) ◽  
pp. 274 ◽  
Author(s):  
Sagar Bashyal ◽  
Avijit Guha
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Chemical Constituents ◽  
Biological Properties ◽  
Phytochemical Analysis ◽  
Aqueous Extracts ◽  
Zone Of Inhibition ◽  
Diffusion Technique ◽  
Trachyspermum Ammi ◽  
Soxhlet Apparatus

 Objective: The objective of this study is to identify medicinally important phytochemicals and evaluate the antimicrobial potential of Trachyspermum ammi seeds. Methods: Four different extracts (methanol, acetone, chloroform, and water) were prepared using a soxhlet apparatus, antimicrobial activity was tested using agar well-diffusion technique. Results: The results revealed the presence of flavonoids and saponins in all the extracts prepared. Similarly, alkaloids and phenols presence were obtained in methanol and aqueous extracts. Glycosides and carbohydrates in methanol, chloroform, and aqueous extracts. Further, proteins, terpenoids, and tannins presence were found in methanol, chloroform, and aqueous extracts, respectively. The maximum zone of inhibition was found in the methanolic extract (13.5 mm). Acetone, chloroform, and water extracts showed 9 mm, 10.5 mm, 11 mm respectively, while ciprofloxacin (control) showed 17.5 mm of the zone of inhibition. Conclusion: T. ammi seeds exert biological properties due to the presence of various chemical constituents. Thus, it can be used to obtain novel antibacterial compounds for the treatment of infectious diseases in the future.

scholarly journals Study on antibacterial activity of Calotropis procera

2014 ◽  
Author(s):  
Muhammad Muzammal
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Salmonella Typhi ◽  
Calotropis Procera ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Crude Extracts ◽  
Mic Value

Comparative study of plant extracts crude and aqueous, methanolic and ethanolic with antibiotics, provide evidence that calotropis procera extracts has the similar antibacterial activity as these antibiotics against test pathogens i.e. Salmonella typhi and E.coli. The analysis of antimicrobial activity of aqueous, methanolic and ethanolic extract of leaves and flower of Calotropis procera was carried out in disc method and also determined MIC value at 600nm through optical density using spectrophotometer. The zone of inhibition produced by extracts was examined and compares it with zone produced by antibiotics. The effect exhibited by ethanolic extract of leaves and flower was significantly greater than the aqueous and methanolic extract of leaves and flower. Crude extracts i.e. latex, leaves, fruit and flower crude extracts. Among them, flower crude extracts shows similar zone of inhibition to test anitibiotics.while in MIC value, we made different concentration of extracts and antibiotics.i.e for crude we made 25%, 50%.75% and 100% concentration of crude juice and for the aqueous, methanolic and ethanolic we made 0.5mg/mL,1mg/mL,2mg/mL,3mg/mL,4mg/mL, 5mg/mL and 6mg/mL in Dimethyl salfoxide, and same antibiotics concentration. Aqueous leaves extracts show MIC at 0.5mg/ml against E.coli while against Salmonella it shows MIC at 1mg/ml... We also determine phytochemical analysis for presences of different compounds in crude extracts. The obtained results provide a support for the use of Calotropis procera, in traditional medicine and suggest its further advance investigation.

scholarly journals Study on antibacterial activity of Calotropis procera

2014 ◽  
Author(s):  
Muhammad Muzammal
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Salmonella Typhi ◽  
Calotropis Procera ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Crude Extracts ◽  
Mic Value

Comparative study of plant extracts crude and aqueous, methanolic and ethanolic with antibiotics, provide evidence that calotropis procera extracts has the similar antibacterial activity as these antibiotics against test pathogens i.e. Salmonella typhi and E.coli. The analysis of antimicrobial activity of aqueous, methanolic and ethanolic extract of leaves and flower of Calotropis procera was carried out in disc method and also determined MIC value at 600nm through optical density using spectrophotometer. The zone of inhibition produced by extracts was examined and compares it with zone produced by antibiotics. The effect exhibited by ethanolic extract of leaves and flower was significantly greater than the aqueous and methanolic extract of leaves and flower. Crude extracts i.e. latex, leaves, fruit and flower crude extracts. Among them, flower crude extracts shows similar zone of inhibition to test anitibiotics.while in MIC value, we made different concentration of extracts and antibiotics.i.e for crude we made 25%, 50%.75% and 100% concentration of crude juice and for the aqueous, methanolic and ethanolic we made 0.5mg/mL,1mg/mL,2mg/mL,3mg/mL,4mg/mL, 5mg/mL and 6mg/mL in Dimethyl salfoxide, and same antibiotics concentration. Aqueous leaves extracts show MIC at 0.5mg/ml against E.coli while against Salmonella it shows MIC at 1mg/ml... We also determine phytochemical analysis for presences of different compounds in crude extracts. The obtained results provide a support for the use of Calotropis procera, in traditional medicine and suggest its further advance investigation.

PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF UDHARA VAYUHARA CHURNA

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (06) ◽  
pp. 64-67
Author(s):  
Maruthi T. Ekbote ◽  
◽  
Rajashekar K. V ◽  
Shankarappa L ◽  
Bharathi D. R. ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Acorus Calamus ◽  
Aqueous Extracts ◽  
Piper Longum ◽  
Polyherbal Formulation ◽  
Methanolic Extracts ◽  
Phytochemical Investigation

Ayurvedic medicines are being used increasingly to fight or prevent common diseases. Udhara vayuhara churna is a polyherbal formulation containing Piper longum (Piperceae) Zingiber officinalis (Zingiberaceae), Elettaria cardamom (Zingiberaceae), Plumbago zeylanica (Plumbaginaceae), Carum carvi (Umbelliferae), Acorus calamus (Araceae) and Embilica officinalis (Euphorbiaceae). Methanolic and aqueous extracts of Udhara vayuhara churna were screened for their antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Aspergillus niger and Candida albicans. The results of antimicrobial activity of aqueous and methanolic extracts of the Udhara vayuhara churna indicated that methanolic extract inhibited the growth of one or more test pathogens than aqueous extract. Udhara vayuhara churna formulation extracts showed a broad spectrum of antimicrobial activity. Phytochemical investigation revealed the presence of tannins, saponins, alkaloids, glycosides, flavonoids and essential oils. This may be due to the multifunctional effect of all the seven plant ingredients of Udhara Vayuhara churn.

scholarly journals Physicochemical Properties and Chemical Constituents of Oil from Joan Seed (Trachyspermum ammi L)

2013 ◽  
Vol 5 (2) ◽  
pp. 15-21
Author(s):  
S Morshed ◽  
MK Alam ◽  
A Begum ◽  
SMS Shahriar ◽  
KN Sharmin ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Chemical Constituents ◽  
Petroselinic Acid ◽  
Acid Value ◽  
Chemical Characteristic ◽  
Local Market ◽  
Gas Liquid Chromatography ◽  
Fatty Oil ◽  
Trachyspermum Ammi ◽  
Soxhlet Apparatus

The paper deals with the investigation of the fatty oil of carom seed (Trachyspermum ammi) for its physicochemical characteristics and fatty acid composition. Oil from carom seed collected from local market was extracted with n-hexane in a glass Soxhlet apparatus. Extracted oil was dark green, burning taste, spicy in odour and soluble in n-hexane, chloroform, pet. ether and benzene. Its chemical characteristic such as acid value (6.69), Iodine value (79.39), Peroxide value (457.11), Saponification value (184.32), Unsaponified matter (9.11), Free fatty acid value as petroselinic acid (48.1) were determined by conventional method. The composition of fatty oil was determined by Gas Liquid Chromatography (GLC). From GLC analysis the main constituents of fatty oil such as Petroselinic acid (89.35%), Oleic acid (5.86%), Linoleic acid (4.79%) were determined. For the better evaluation of these spices, it is necessary to know their complete chemical composition.DOI: http://dx.doi.org/10.3329/jesnr.v5i2.14571 J. Environ. Sci. & Natural Resources, 5(2): 15-21 2012

scholarly journals High Performance Thin Layer Chromatography Fingerprinting Analysis of Piper betle L. Leaves

2021 ◽  
pp. 8-15
Author(s):  
Ramdas N. Kale ◽  
Ravindra Y. Patil
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Chemical Constituents ◽  
Piper Betle ◽  
Soxhlet Apparatus ◽  
Rf Values ◽  
Pharmacologically Active ◽  
Layer Chromatography

Introduction: Many modern medicines used today based on plants and plant products. Piper betle is generally known as the betle vine, it is an important medicinal and recreational plant. High performance thin layer chromatography (HPTLC) is an advanced powerful analytical method with more separation power, high performance and superior reproducibility than classic thin layer chromatography (TLC). A chromatographic fingerprint of a plant extract is a chromatographic pattern of some common chemical constituents of pharmacologically active and/or chemical characteristics. Chromatographic fingerprints are useful in authentication and identification of plant. Objectives:  Objectives of present research was to establish HPTLC fingerprinting of methanolic extract of Piper betle L. leaves. Materials and Methods: Methanolic extract of Piper betle leaves was prepared using soxhlet apparatus. HPTLC studies were performed using a CAMAG HPTLC system equipped with automatic TLC sampler-4 (ATS 4), TLC scanner 4, and vision CATS 3.0 software. Results: The study revealed the presence of alkaloids with Rf value 0.65, flavonoids with Rf values 0.19, 0.29, 0.72, 0.95., and phenolic compound with Rf value 0.7. Conclusion: The HPTLC fingerprinting profile developed for the methanolic extract of Piper betle L. leaves will help in proper identification of the plant.Piper betle

scholarly journals Antimicrobial Activity and HPLC Fingerprinting of CrudeOcimumExtracts

2011 ◽  
Vol 8 (3) ◽  
pp. 1430-1437 ◽  
Author(s):  
S. S. Deo ◽  
F. Inam ◽  
R. P. Mahashabde
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Ocimum Sanctum ◽  
Moderate Activity ◽  
Aqueous Extracts ◽  
Gram Positive ◽  
Strong Activity ◽  
Gram Negative ◽  
E Coli ◽  
Strong Antimicrobial Activity

The antimicrobial activity of crude methanolic and aqueous extracts ofOcimum sanctumandOcimum kilimandsacharicumagainst gram positive, gram negative and antifungal activity was evaluated to find the zone of inhibition and to set a HPLC profile or fingerprint of these extracts. The crude methanolic extract ofOcimum sanctumshowed strong antimicrobial activity againstS.aureusandC. albicansand moderate activity againstE. coliandB. subtilis. The crude methanolic extract ofOcimum kilimandsacharicumshowed strong antimicrobial activity againstS. aureus, E. coliandC. albicansat higher concentration, same as that shown by the standard forC. albicans. It showed moderate activity againstB. subtilis. The crude aqueous extracts of Ocimum sanctum showed strong antimicrobial activity againstS.aureusand moderate against others. Whereas the crude aqueous extracts ofOcimum kilimandsacharicumshowed moderate activity against the gram positive and gram negative organisms and strong activity againstC. albicansat higher concentration, same as that shown by the standard forC. albicans.

scholarly journals Study of Isolation, Characterisation and Antimicrobial Activity of High Value Bioactive Compounds from Methanolic Extract of Leaves of Tilkor (Momordica Monadelpha)

2018 ◽  
Vol 34 (6) ◽  
pp. 3145-3152
Author(s):  
Panshu Pratik ◽  
Prem Mohan Mishra
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Soxhlet Extraction ◽  
Extraction Process ◽  
Phytochemical Analysis ◽  
Spectroscopic Techniques ◽  
Compound A ◽  
Tropical Conditions ◽  
Isolated Compound ◽  
Layer Chromatography

In this paper an attempt has been made to highlight the physicochemical study of methnolic extract of leaves of Tilkor carried out by soxhlet extraction process, phytochemical analysis of the extract, separations, isolation of bioactive components through Thin Layer Chromatography (TLC) as well as column chromatography respectively and characterisation of isolated compound by the means of several spectral analysis such as 1H NMR, 13C NMR, IR, U.V. Mass spectroscopy. The methanolic extract of leaves of the plant (in tropical conditions of Mithilanchal, Bihar, India) reveal the presence of phytochemicals like alkaloids, flavanoids, tannins, saponins, cardiac glycosides, steroids, terpenoids etc. The secondary metaboilities showed antimicrobial activity. The two isolated compounds were characterised by spectroscopic techniques which revealed the structure of compound A as - stigmosterol and compound B as tritriaconatane and is also found to have antimicrobial activity.

scholarly journals Phytochemical characterization, anti-cancer and antimicrobial activity of isolated fractions of Alysicarpus vaginalis

2017 ◽  
Vol 12 (1) ◽  
pp. 77 ◽  
Author(s):  
Ganesh Tapadiya ◽  
Mayura A. Kale ◽  
Shweta Saboo
Keyword(s):  
Antimicrobial Activity ◽  
Cell Lines ◽  
Methanolic Extract ◽  
Hep G2 ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Anti Cancer ◽  
Proliferative Assay ◽  
Bacteria And Fungi

<p class="Abstract">The methanolic extract of <em>Alysicarpus </em>vaginalis was selected for fractionation due to its known reported biological activity. The four fractions were separated and subjected for<em> in vitro</em> antimitotic and anti-proliferative assays along with anti-cancer activity on two human cancers cell lines (SK-MEL-2 and Hep-G2). The antimicrobial potential of fractions had been evaluated against bacteria and fungi. From all fractions, acetone and n-butanol fractions were effective against the cell lines. They show strong inhibitory action with mitotic index 6.2 and 8.4 mg/mL and IC<sub>50 </sub>values of anti-proliferative assay in between 19.7 to 14.2 mg/mL respectively, which was found to be comparable to the standard methothrexate 5.9 mg/mL and 13.2 mg/mL respectively. In antimicrobial activity, the zone of inhibition had been observed in the range of 12-27 mm and MIC value was found in the range of 0.2-0.1 mg/mL. The acetone fraction was found to be most active against fungi, and<em> E. coli</em> whereas chloroform and n-butanol fractions were more effective against <em>S. aureus</em> and <em>B. </em>subtilis. The phytochemical characterization by HPLC analysis indicated the presence of important polyphenolic and steroidal compounds.</p>

scholarly journals Evaluation of Antimicrobial Activity and Synergistic Effect of Spices against Few Selected Pathogens

2019 ◽  
Vol 6 ◽  
pp. 10-18
Author(s):  
Renuka Maharjan ◽  
Saru Thapa ◽  
Amrit Acharya
Keyword(s):  
Antimicrobial Activity ◽  
Synergistic Effect ◽  
Inhibitory Concentration ◽  
Agar Plate ◽  
Ethanolic Extract ◽  
Fractional Inhibitory Concentration ◽  
Zone Of Inhibition ◽  
Soxhlet Apparatus ◽  
Mic Value

Objectives: The main objective of this study was to evaluate antimicrobial activity of ethanolic extract of spices along with determination of its synergistic effect against few selected pathogens. Methods: In this study, ethanolic extract of 5 different spices; Zingiber officinale (Ginger), Allium sativum (Garlic), Curcuma longa (Turmeric), Capsicum annum (Chili) and Allium cepa (Onion) were obtained by using Soxhlet apparatus. The ethanolic extract was concentrated by evaporation and different concentrations of extract were prepared in Dimethy Sulphoxide (DMSO) solvent. Test organisms included mainly pathogens i.e. Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae. The antimicrobial activities of the extracts were determined by well diffusion technique both individually and in combination. On the other hand, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) was determined by serial dilution technique. The result were interpreted on the basis of the fact that the growth occurs in positive control and other tubes with inadequate amount of extract whereas the lowest concentration of agent that inhibits growth of organism, detected by lack of visible turbidity by inhibition of 99% is designed as the MIC. The MBC is identified by determining the lowest concentration of extract solution that reduces the viability of the initial bacterial inoculum by a predetermined reduction such as ≥99.9%. Likewise, for determination of Fractional Inhibitory Concentration Index (FICI), two extracts were combined along with standardized inoculum of bacterial strain. Tubes without visible turbidity were streaked on agar plate and observed for 99.9% killing.   Results: All the tested extract of spices were found effective against S. aureus and K. pneumoniae only. The highest zone of inhibition (ZOI) was found in chili extract (ZOI=26 mm) against S. aureus whereas lowest zone of inhibition was found in garlic extract against K. pneumoniae (ZOI=12mm). Similarly, highest ZOI was produced by combined extract of both Turmeric and Ginger (ZOI= 26 mm). Turmeric extract was found to be effective against S. aureus (MIC value = 62.5 mg /ml and MBC value = 31.25 mg/ml) and K. pneumoniae (MIC value 125 mg/ml and MBC value = 62.5 mg/ml). The Fractional Inhibitory Concentration (FIC) values of combined extract suggested synergistic and additive effect (0.5<FIC<1). Chili and ginger were effective with FIC value of 0.25. Conclusion: To recapitulate, the extract of spices can be used to prevent the pathogenic organism.

Antimicrobial Activity of Calotropis gigantea against Staphylococcus aureus: Eco-Friendly Management

2020 ◽  
Vol 6 (01) ◽  
pp. 94-98
Author(s):  
Madhu Praskash Srivastava ◽  
Kanchan Awasthi ◽  
Pratibha Kumari
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Quantitative Analysis ◽  
Neurological Disorders ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Active Compounds ◽  
Calotropis Gigantea ◽  
Aqueous Method ◽  
Different Types

Calotropis gigantea (Madaar) Linn. is a potent medicinal herb that has active compounds in the form of alkaloids, glycosides, lactones, and steroids. All these active compounds have immune-modulatory and physiological roles of different types; the plant is also reported as effective in treating skin, digestive, respiratory, circulatory, and neurological disorders and was used to treat fevers, elephantiasis, nausea, vomiting, and diarrhea. In this study, the phytochemical and the zone of inhibition was measured in C. gigantea L. It proves that C. gigantea L. is more effective against Staphylococcus aureus. Aqueous extract of C. gigantea L. was extracted by the aqueous method. In the qualitative phytochemical analysis presence of various secondary metabolites were found as alkaloids, flavonoids, tannins, and saponin. In the quantitative analysis, carbohydrate was found in C. gigantea L. about concentration is found (470 μg/mL). Antimicrobial activity was also quite good against S. aureus. The study demonstrates that the C. gigantea L. contains the presence different of bioactive compounds indicated a potent antimicrobial activity of C. gigantea L. against S. aureus so that we called as eco-friendly management.

Sign in / Sign up

Export Citation Format

Share Document