scholarly journals ISOLATION OF A COMPOUND FROM THE BULBS OF ELEUTHERINE BULBOSA (MILLER) URBAN (IRIDACEAE)

2018 ◽  
Vol 11 (6) ◽  
pp. 406
Author(s):  
Rani Vs
Keyword(s):  
Ftir Spectroscopy ◽  
Spectroscopic Analysis ◽  
Soxhlet Extraction ◽  
Bioactive Compound ◽  
Structural Elucidation ◽  
Extraction Column ◽  
Liquid Chromatography Mass Spectrometry ◽  
1H Nuclear Magnetic Resonance ◽  
Isolated Compound ◽  
Layer Chromatography

 Objective: The present study is concerned with the detection, isolation, identification, and structural elucidation of the bioactive phytochemical from the bulbs of Eleutherine bulbosa.Methods: Ethyl acetate is the solvent used in Soxhlet extraction. Column chromatography and thin layer chromatography is employed in the isolation procedure. Identification and structural elucidation of the bioactive compound is determined using ultraviolet-visible (UV-vis), Fourier transform-infrared (FTIR) spectroscopy, liquid chromatography–mass spectrometry (LCMS-MS), and 1H nuclear magnetic resonance (1HNMR) spectroscopy.Results: A compound was isolated from the bulbs of E. bulbosa by solvent extraction and chromatography. UV-vis, FTIR spectroscopy, LCMS-MS, and 1HNMR studies showed that the isolated compound was bis (2-6,7-diamino-5,8-dioxo naphthalene-1yl) propanal.Conclusion: An yellowish-brown crystalline needle-shaped compound was isolated from the bulbs of E. bulbosa and characterized chemically. Spectroscopic analysis identified the compound as bis (2-6,7-diamino-5,8-dioxo naphthalene-1yl) propanal. Mass of the compound is m/z 244 (MH+=245).

scholarly journals Study of Isolation, Characterisation and Antimicrobial Activity of High Value Bioactive Compounds from Methanolic Extract of Leaves of Tilkor (Momordica Monadelpha)

2018 ◽  
Vol 34 (6) ◽  
pp. 3145-3152
Author(s):  
Panshu Pratik ◽  
Prem Mohan Mishra
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Soxhlet Extraction ◽  
Extraction Process ◽  
Phytochemical Analysis ◽  
Spectroscopic Techniques ◽  
Compound A ◽  
Tropical Conditions ◽  
Isolated Compound ◽  
Layer Chromatography

In this paper an attempt has been made to highlight the physicochemical study of methnolic extract of leaves of Tilkor carried out by soxhlet extraction process, phytochemical analysis of the extract, separations, isolation of bioactive components through Thin Layer Chromatography (TLC) as well as column chromatography respectively and characterisation of isolated compound by the means of several spectral analysis such as 1H NMR, 13C NMR, IR, U.V. Mass spectroscopy. The methanolic extract of leaves of the plant (in tropical conditions of Mithilanchal, Bihar, India) reveal the presence of phytochemicals like alkaloids, flavanoids, tannins, saponins, cardiac glycosides, steroids, terpenoids etc. The secondary metaboilities showed antimicrobial activity. The two isolated compounds were characterised by spectroscopic techniques which revealed the structure of compound A as - stigmosterol and compound B as tritriaconatane and is also found to have antimicrobial activity.

Seasonal variation of abscisic acid in the dormant shoots of Douglas-fir

1979 ◽  
Vol 57 (5) ◽  
pp. 534-538 ◽  
Author(s):  
Joe E. Webber ◽  
Murray L. Laver ◽  
Joe B. Zaerr ◽  
Denis P. Lavender
Keyword(s):  
Abscisic Acid ◽  
Liquid Chromatography ◽  
Seasonal Variation ◽  
Internal Standard ◽  
Close Correlation ◽  
Douglas Fir ◽  
Gas Liquid Chromatography ◽  
Bud Burst ◽  
Liquid Chromatography Mass Spectrometry ◽  
Layer Chromatography

The occurrence of abscisic acid (ABA) in the dormant shoots of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) was confirmed by bioassay, thin-layer chromatography, gas–liquid chromatography, and gas–liquid chromatography – mass spectrometry. Seasonal variation of ABA in the buds, leaves, and stems was then determined using 2-trans-ABA as an internal standard. Concentrations of ABA were highest in the autumn for buds (2.1 μg/g) and needles (0.79 μg/g) and highest in January for stems (0.34 μg/g). The lowest concentrations for all tissues were in February and March, before bud burst. Close correlation of levels of ABA with previously measured physiological evidence of growth and metabolic activity suggests a possible role in the dormancy cycle of Douglas-fir.

scholarly journals Optimization of Gamma Aminobutyric Acid Production Using High Pressure Processing (HPP) by Lactobacillus brevis PML1

2022 ◽  
Vol 2022 ◽  
pp. 1-9
Author(s):  
Atefe Ghafurian Nasab ◽  
Sayed Ali Mortazavi ◽  
Farideh Tabatabaei Yazdi ◽  
Mahboobe Sarabi Jamab
Keyword(s):  
High Performance ◽  
Morphological Changes ◽  
Control Sample ◽  
Bioactive Compound ◽  
Lactobacillus Brevis ◽  
High Pressure Processing ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Viability Analysis ◽  
Layer Chromatography

In the present research, the production potential of gamma aminobutyric acid (GABA) using Lactobacillus brevis PML1 was investigated. In addition, the microorganism viability was examined in MAN, ROGOSA, and SHARPE (MRS) after undergoing high hydrostatic pressure at 100, 200, and 300 MPa for 5, 10, and 15 min. Response surface methodology (RSM) was applied to optimize the production conditions of GABA as well as the bacteria viability. Analysis of variance (ANOVA) indicated that both the independent variables (pressure and time) significantly influenced the dependent ones (GABA and bacteria viability) ( P < 0.05 ). The optimum extraction conditions to maximize the production of GABA included the pressure of 300 MPa and the time of 15 min. The amount of the compound was quantified using thin-layer chromatography (TLC) and spectrophotometry. For the process optimization, a central composite design (CCD) was created using Design Expert with 5 replications at the center point, whereby the highest content of GABA was obtained to be 397.73 ppm which was confirmed by high performance liquid chromatography (HPLC). Moreover, scanning electron microscopy (SEM) was utilized to observe the morphological changes in the microorganism. The results revealed that not only did have Lactobacillus brevis PML1 the potential for the production of GABA under conventional conditions (control sample) but also the content of this bioactive compound could be elevated by optimizing the production parameters.

scholarly journals Chemical constituents of Euphorbia tirucalli L.

2019 ◽  
Vol 2 (5) ◽  
pp. 76-82
Author(s):  
Dung Thi Kim Le ◽  
Hao Xuan Bui ◽  
Tuyet Thi Anh Nguyen ◽  
Tuyen Nguyen Kim Pham ◽  
Huy Thuc Duong
Keyword(s):  
Thin Layer ◽  
Gallic Acid ◽  
Spectroscopic Analysis ◽  
Chemical Constituents ◽  
Normal Phase ◽  
Chemical Structures ◽  
Euphorbia Tirucalli ◽  
Arjunolic Acid ◽  
First Time ◽  
Layer Chromatography

Euphorbia tirucalli has not been chemically studied much in Vietnam. This research described the isolation and elucidation of compounds isolated from the plant collected in Binh Thuan. Multiple chromatographic methods were applied, including normal phase silica gel column chromatography and thin-layer chromatography. Seven compounds were isolated and their chemical structures were elucidated by spectroscopic analysis as well as comparing their data with the ones in the literature. They are arjunolic acid (1), eriodictyol (2), quercitrin (3), afzelin (4), scopoletin (5), 3,3′,4- trimethylellagic acid (6), and gallic acid (7). Among them, compound 1 a major component was isolated for the first time in Euphorbia genus, while three compounds 2, 4, and 5 were isolated from this species for the first time.

scholarly journals The phytochemical screenings and thin layer chromatography analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.)

2005 ◽  
Vol 3 (1) ◽  
pp. 26-31 ◽  
Author(s):  
SOERYA DEWI MARLIANA ◽  
VENTY SURYANTI ◽  
SUYONO SUYONO
Keyword(s):  
Thin Layer ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
Soxhlet Extraction ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Thin Layer Chromatography Analysis ◽  
Chromatography Analysis ◽  
Sechium Edule ◽  
Layer Chromatography

The phytochemical screenings and analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.) with Thin Layer Chromatography (TLC) has been carried out. Isolation was done by Soxhlet extraction for 6 hours with petroleum ether and the residue was extracted by maceration during 24 hours with ethanol.The isolated compounds in ethanol extract were identified by phytochemical screenings method and TLC. The result showed the presence of alkaloid, saponin, cardenolin/bufadienol and flavonoid.

Key factors in FTIR spectroscopic analysis of DNA: the sampling technique, pretreatment temperature and sample concentration

2018 ◽  
Vol 10 (21) ◽  
pp. 2436-2443 ◽  
Author(s):  
Yahong Han ◽  
Lujia Han ◽  
Yumei Yao ◽  
Yanfei Li ◽  
Xian Liu
Keyword(s):  
Molecular Weight ◽  
Fourier Transform ◽  
Ftir Spectroscopy ◽  
Deoxyribonucleic Acid ◽  
Spectroscopic Analysis ◽  
Sampling Technique ◽  
Key Factors ◽  
Sample Concentration ◽  
Pretreatment Temperature ◽  
Molecular Weight Of Dna

Fourier transform infrared (FTIR) spectroscopy has been considered as a powerful tool for analysing the characteristics of deoxyribonucleic acid (DNA) regardless of physical states, sample amounts and the molecular weight of DNA.

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