scholarly journals EFFECT OF SITAGLIPTIN AND VILDAGLIPTIN ON WOUND HEALING IN MALE WISTAR RATS - AN EXPERIMENTAL STUDY

2018 ◽  
Vol 11 (8) ◽  
pp. 392
Author(s):  
Netravathi B Angadi ◽  
Urmila Kagal ◽  
Somaling Timshetti
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Dead Space ◽  
Wistar Rats ◽  
Healing Process ◽  
Dry Weight ◽  
Morbidity And Mortality ◽  
Wound Model ◽  
Male Wistar Rats ◽  
Histopathological Studies

Objective: Diabetes mellitus (DM) is a spectrum of common metabolic disorders, arising from a variety of pathogenic mechanisms. With an increasing incidence worldwide, DM will be likely a leading cause of morbidity and mortality in the future. Delayed wound healing in diabetes is a major source of morbidity and mortality. Sitagliptin and Vildagliptin are novel antihyperglycemic agents used for the treatment of DM. The present study was planned to investigate the effect of Sitagliptin and Vildagliptin on various wound healing models in male Wistar rats.Methods: Male Wistar rats (150–200 g) were divided into three groups, i.e., control, Sitagliptin, and Vildagliptin (n=6 animals in each group) for each wound model. Excision wound, resutured incision wound, and dead space wounds were inflicted under thiopentone anesthesia in male Wistar rats. The rats received Sitagliptin and Vildagliptin orally during the study period. Resutured incision was assessed by wound breaking strength; dead space wound was assessed by granuloma dry weight and histopathology of granulation tissue. In excision wounds, treatment was monitored by planimetry. Data were expressed as mean±standard error of mean and analyzed by analysis of variance followed by Dunnett’s post hoc test. p<0.05 was considered statistically significant.Results: Sitagliptin and Vildagliptin significantly promoted the healing process in all three wound models studied. Histopathological studies revealed increased collagen content and granulation tissue in Sitagliptin and Vildagliptin groups.Conclusion: In all the three wound models studied, Sitagliptin and Vildagliptin promoted wound healing. The pro-healing effect of Sitagliptin and Vildagliptin needs to be explored clinically.

scholarly journals PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

2015 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Umi Kulsum ◽  
Ratnawati Hendari ◽  
Siti Chumaeroh
Keyword(s):  
Wound Healing ◽  
Ulcer Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Healing Process ◽  
Aloe Vera ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

scholarly journals Studies on Wound Healing Activity of Heliotropium indicum Linn. Leaves on Rats

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
G. K. Dash ◽  
P. N. Murthy
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Dead Space ◽  
Methanol Extract ◽  
Infection Model ◽  
Aqueous Extracts ◽  
Reference Standard ◽  
Wound Model ◽  
Heliotropium Indicum ◽  
Wound Healing Activity

The petroleum ether, chloroform, methanol, and aqueous extracts of Heliotropium indicum Linn. (Family: Boraginaceae) were separately evaluated for their wound healing activity in rats using excision (normal and infected), incision, and dead space wound models. The effects of test samples on the rate of wound healing were assessed by the rate of wound closure, period of epithelialisation, wound breaking strength, weights of the granulation tissue, determination of hydroxyproline, super oxide dismutase (SOD), catalase, and histopathology of the granulation tissues. Nitrofurazone (0.2% w/w) in simple ointment I. P. was used as reference standard for the activity comparison. The results revealed significant promotion of wound healing with both methanol and aqueous extracts with more promising activity with the methanol extract compared to other extracts under study. In the wound infection model (with S. aureus and P. aeruginosa), the methanol extract showed significant healing activity similar to the reference standard nitrofurazone. Significant increase in the granulation tissue weight, increased hydroxyproline content, and increased activity of SOD and catalase level with the animals treated with methanol extract in dead space wound model further augmented the wound healing potential of H. indicum. The present work substantiates its validity of the folklore use.

scholarly journals Neutrophil count in the gingival wound healing process after apitoxin gel application (gingival wound healing model on Wistar rats)

2021 ◽  
Vol 6 (2) ◽  
pp. 65
Author(s):  
Urfa Tabtila ◽  
Swastiana Eka Yunita ◽  
Muhammad Nabil Pratama ◽  
Juni Handajani
Keyword(s):  
Wound Healing ◽  
Periodontal Disease ◽  
Neutrophil Count ◽  
Wistar Rats ◽  
Healing Process ◽  
Inflammatory Cells ◽  
Punch Biopsy ◽  
Bee Venom ◽  
Wound Healing Process ◽  
Male Wistar Rats

Periodontal disease has a high prevalence in Indonesia. Gingival disease is a periodontal disease if it is severe, requires surgical therapy called a gingivectomy. Surgical periodontal procedures lead to a gingival wound that is usually treatedusing a periodontal pack. Periodontal pack has no curative property in general but assists in a wound healing process by protecting tissues. Neutrophils are inflammatory cells that act as a host’s first defense against infectious pathogens and they are important in wound healing. Wound healing can be accelerated by apitoxin gel from honeybee Apis mellifera species. The aim of this study was to determine the effectiveness of apitoxin gel on neutrophil count on gingival woundhealing process. Medical bee venom powder (Umea, Sweden) was mixed with double-distilled water to prepare a concentration of 0.01% bee venom. The solution was mixed with 10% propylene glycol, 0.01% methylparaben, and3% CMC-sodium. Thirty six gingiva of 3 month-old male Wistar rats (weighing 200-250 gram) were injured using punch biopsy (3 mm in diameter). They were treated in 4 groups: apitoxin gel, apitoxin gel with a periodontal pack, NSAIDsFlamar® gel with periodontal pack, and a periodontal pack. Three Wistar rats from each group were euthanized and their jaws were taken after 48 hours treatment to make histology slides with Hematoxylin Eosin (HE) staining. Neutrophil counts were observed. The data were analyzed using Anova test with 95% confidence level. The results showed there were significan defferebces among 4 groups (p<0.05). The highest neutrophil count on the 2nd day was found in the apitoxin gel induced the 2 day of gingival wound healing process.

scholarly journals The Effect of Betel Quid Extract on Wound Healing Process in Male Wistar Rats (Rattus norvegicus L.)

2019 ◽  
Vol 7 (6) ◽  
Author(s):  
Siti Rusdiana Puspa Dewi ◽  
Adelina Fatonah
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Positive Control ◽  
Betel Quid ◽  
Wound Healing Process ◽  
Lower Lip ◽  
Proliferative Phase ◽  
Inflammatory Phase ◽  
Male Wistar Rats

Wound can be occured during dental treatment. In outline, there are several phases of wound healing; inflammatory phase, proliferative phase, and maturation phase. Various drugs in various forms are used to accelerate the healing process, but mostly they have side effects. Therefore, traditional medicine, such as betel quid consisted of betel leaf, areca nut, gambier, and calcium hydroxide, is developed. The aim of this study was to investigate the effect of wound healing process in male Wistar rats. A total of 30 male Wistar rats were taken and divided into 5 groups:  Group 1, 2, and 3 (K1,K2, K3)  were given 5%, 10% and 15% concentrations of betel quid extract oinments; Group 4 (K4) was positive control  (hyaluronic acid 0.2% oinment); Group 5 (K5) was negative control (placebo oinment). One mm- diameter of lower lip mucosal wounds on rats were created by using  cylinder diamond bur. The oinments were applied twice daily for 10 days. The number of netrophils on first and third day were measured and the thickness of ephitelium on 10 days were determined. All groups of  betel quid extracts exhibited the reduction of the number of neutrophils on inflammatory phase. Group 3 shown as the highest effect and had no significant different with positive control. Betel quid extracts in all groups also improved epithelial thickness on proliferative phase, in which group II and group III had no significant different with positive control. Betel quid extract had effect on wound healing process in male Wistar rats due to its ability in supressing inflamation and in increasing reepithelization.

scholarly journals EFFECT OF CHANNA MICROPELTES IN THE GRANULATION, FIBROSIS AND NECROSIS OF DIABETIC WOUND HEALING

2021 ◽  
Vol 6 (2) ◽  
pp. 171
Author(s):  
Amy Nindia Carabelly ◽  
Juliyatin Putri Utami ◽  
Didit Aspriyanto ◽  
Muhammad Hasanu Reksi ◽  
Dewi Puspitasari ◽  
...  
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Wistar Rats ◽  
Healing Process ◽  
Wound Complications ◽  
Control Group ◽  
Diabetic Wound ◽  
Model Group ◽  
Delayed Wound Healing ◽  
Diabetic Model

Background: Diabetes mellitus (DM) is characterized by persistent hyperglycemia. The symptoms of DM is delayed wound healing. Delayed wound healing in diabetes will increase the risk of wound complications that will hinder the healing process, and lead to complications such as fibrosis and necrosis. The use of Channa Micropeltes (CM) extract at a dose of 16 mL/kg BW orally is proven to general optimal wound closure and wound contraction clinically in Wistar rats as diabetic model on day 14.However, the histopathological description of wound healing is yet to be known. Objective: To analyze the effect of 16 mL/kg BW dose of CM extract on histopathological changes of wound healing granulation tissue, fibrosis and necrosis in diabetic Wistar rats on day 14. Materials and Methods: This study was a true experimental design with a post-test only control group design. The samples were divided into two groups, namely the diabetic model group given CM extract at 16 mL/kg BW dose for 14 days, and the diabetic model group given BR2 feed only for 14 days. Results: The results of Mann-Whitney U test showed p = 0.003 for granulation tissue, p = 0.411 for fibrosis and p = 1 for necrosis, with the level of significance was p<0.05. Conclusion: The CM extract at 16 mL/kg BW dose affects the histopathological description of granulation tissue which presents a better result than those in the control group but did not affect the features of fibrosis and necrosis in diabetic Wistar rats on day 14. Keywords: Channa Micropeltes, Diabetic wound, Fibrosis, Granulation tissue, Necrosis.

scholarly journals FORMULATION OF GEL FROM GYNURA SEGETUM EXTRACT AND ITS ACTIVITY ON BURN WOUND HEALING

2021 ◽  
pp. 269-271
Author(s):  
ARIF BUDIMAN ◽  
MUHARAM ◽  
ANIDA CRISTI MAULIDA ◽  
DIAH LIA AULIFA
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Physical Stability ◽  
Wound Healing Process ◽  
Burn Wound ◽  
Hot Plate ◽  
Topical Preparation ◽  
Burn Wound Healing ◽  
Male Wistar Rats

Objective: The aims of this study were to formulate gel from Gynura segetum (GS) extract and evaluate its burn-healing activity. Methods: GS extract was formulated using carbomer and carboxymethylcellulose (CMC) as a stabilizer with various concentrations. Furthermore, the gel of GS extract was evaluated, including organoleptic, pH and viscosity. A burn-healing evaluation was conducted by making a wound with a hot plate on male Wistar rats, and 600 mg of the gel was applied. Subsequently, the presence of erythema and width contraction of the burns were observed for 15 d. Results: The result showed that the formulation containing 1 % of carbomer and 1.2 % of GS extract has the best physical stability. The gel also increased the rate of the healing process with decreased burn wound contraction (5.67 mm after 15 d) and the erythema than the control (8.50 mm after 15 d). The significance value was less than 0.05, indicating that the rate of the healing process was significantly different between the GS extract gel and the control. Conclusion: This finding demonstrated that the gel of GS extract can significantly improve the burns wound healing process and may also be safely used for topical preparation.

scholarly journals Injectable Self-Healing Adhesive pH-Responsive Hydrogels Accelerate Gastric Hemostasis and Wound Healing

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Jiahui He ◽  
Zixi Zhang ◽  
Yutong Yang ◽  
Fenggang Ren ◽  
Jipeng Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Endoscopic Treatment ◽  
Healing Process ◽  
Gelation Time ◽  
Type I ◽  
Self Healing ◽  
Ph Responsive ◽  
Gastric Hemorrhage ◽  
Wound Model ◽  
Arterial Bleeding

AbstractEndoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD) are well-established therapeutics for gastrointestinal neoplasias, but complications after EMR/ESD, including bleeding and perforation, result in additional treatment morbidity and even threaten the lives of patients. Thus, designing biomaterials to treat gastric bleeding and wound healing after endoscopic treatment is highly desired and remains a challenge. Herein, a series of injectable pH-responsive self-healing adhesive hydrogels based on acryloyl-6-aminocaproic acid (AA) and AA-g-N-hydroxysuccinimide (AA-NHS) were developed, and their great potential as endoscopic sprayable bioadhesive materials to efficiently stop hemorrhage and promote the wound healing process was further demonstrated in a swine gastric hemorrhage/wound model. The hydrogels showed a suitable gelation time, an autonomous and efficient self-healing capacity, hemostatic properties, and good biocompatibility. With the introduction of AA-NHS as a micro-cross-linker, the hydrogels exhibited enhanced adhesive strength. A swine gastric hemorrhage in vivo model demonstrated that the hydrogels showed good hemostatic performance by stopping acute arterial bleeding and preventing delayed bleeding. A gastric wound model indicated that the hydrogels showed excellent treatment effects with significantly enhanced wound healing with type I collagen deposition, α-SMA expression, and blood vessel formation. These injectable self-healing adhesive hydrogels exhibited great potential to treat gastric wounds after endoscopic treatment.

Biochemical and histopathological effects of low dose vanadium in the healing of acetic acid-induced colitis in male wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Tosan Peter Omayone ◽  
Samuel Babafemi Olaleye
Keyword(s):  
Acetic Acid ◽  
Untreated Control ◽  
Wistar Rats ◽  
Low Dose ◽  
Healing Process ◽  
Beneficial Effects ◽  
Male Wistar Rats ◽  
Haematological Changes ◽  
Therapeutic Properties ◽  
Histopathological Effects

Abstract Objectives Vanadium has been reported to possess relevant therapeutic properties such as anti-diabetic and anti-tumoral. This study aimed at determining the effects of vanadium on experimentally induced colitis in rats. Methods Forty-five male Wistar rats (103 ± 3.90 g, n=15) were used for this study and were divided into three groups. Group 1 (Untreated control) had nothing added to their drinking, while groups 2 and 3 received sodium metavanadate at a dose of 50 and 200 mg/L respectively in their drinking water for 10 weeks. Colitis was thereafter induced by intra colonic administration of 1.50 mL of 6% acetic acid. Animals were sacrificed on day 0 (pre-induction), three- and seven-days post induction. Blood samples were collected for haematological variables and the distal 8 cm of the colon was collected for macroscopic, histological and biochemical (malondialdehyde-MDA, superoxide dismutase-SOD, catalase-CAT, glutathione peroxidase- GPx and nitrite concentration- NO) assessment. Results Low dose vanadium proved beneficial in ameliorating acetic acid-induced colitis by improving both histopathological and haematological changes. Gross observation showed a faster healing rate in vanadium treated groups (50 and 200 mg/L) compared with untreated control at day 3 (40 and 26.20 vs. 2.50%) and day 7 (80 and 66.70 vs. 42%) respectively. Vanadium also appears to exert its beneficial effects on acetic acid-induced colitis via up regulation of antioxidant enzymes (SOD, CAT, GPx) and NO while decreasing the over production of MDA. Conclusions Vanadium at small concentration functions as an essential trace element and may be able to promote healing process during ulcerative colitis.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals EFFECT OF HEALOL OIL ON WOUND HEALING IN WISTAR RATS: A NOVEL STUDY

2018 ◽  
Vol 11 (3) ◽  
pp. 152
Author(s):  
Sujith V ◽  
Poornima G ◽  
Balaji O ◽  
Bairy Kl ◽  
Praveen K ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Chronic Wounds ◽  
Statistical Significance ◽  
Silver Sulfadiazine ◽  
Histopathological Analysis ◽  
Burn Wound ◽  
Excision Wound ◽  
Burn Wounds ◽  
Wound Model

 Objective: Wound healing is a complex process, and various plant extracts have been used to study the effect of medicinal plants on wound healing. Healol oil is used in some tribal areas for ulcer healing and treatment of burn wounds. There are no animal studies done so far using healol oil to find out its role in the treatment of wounds. Hence, the aim of our study is to find the effect of healol oil in excision as well burn wounds in Wistar rats.Methods: Thirty-six Wistar rats were used, 18 rats in excision wound model and remaining in burn wound model. Each model consists of three groups of six rats each. Povidone-iodine was used as a standard control in excision wound model and silver sulfadiazine was used as a standard control in burn wound model. Healol oil was used as test drug in both the models. Period of epithelization and wound contracture rates were analyzed. Histopathological analysis of the skin tissue was done. One-way analysis of variance was used followed by Tukey’s post hoc test for statistical analysis using SPSS version 23 software, P value of <0.05 was taken as statistical significance.Results: Both standard and test groups showed significantly faster wound healing (p<0.001) compared to control rats treated with paraffin wax in both the models. In excision model standard povidone was significantly (p<0.001) better compared to healol oil whereas in burn model, silver sulfadiazine and healol oil showed comparable results with respect to period of epithelization without statistical significance (p>0.05).Conclusion: Wound healing property of healol oil is proved, and further clinical trial can be done to find out the effect of healol oil on chronic wounds due to various etiologies.

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