scholarly journals EFFECT OF CHANNA MICROPELTES IN THE GRANULATION, FIBROSIS AND NECROSIS OF DIABETIC WOUND HEALING

2021 ◽  
Vol 6 (2) ◽  
pp. 171
Author(s):  
Amy Nindia Carabelly ◽  
Juliyatin Putri Utami ◽  
Didit Aspriyanto ◽  
Muhammad Hasanu Reksi ◽  
Dewi Puspitasari ◽  
...  
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Wistar Rats ◽  
Healing Process ◽  
Wound Complications ◽  
Control Group ◽  
Diabetic Wound ◽  
Model Group ◽  
Delayed Wound Healing ◽  
Diabetic Model

Background: Diabetes mellitus (DM) is characterized by persistent hyperglycemia. The symptoms of DM is delayed wound healing. Delayed wound healing in diabetes will increase the risk of wound complications that will hinder the healing process, and lead to complications such as fibrosis and necrosis. The use of Channa Micropeltes (CM) extract at a dose of 16 mL/kg BW orally is proven to general optimal wound closure and wound contraction clinically in Wistar rats as diabetic model on day 14.However, the histopathological description of wound healing is yet to be known. Objective: To analyze the effect of 16 mL/kg BW dose of CM extract on histopathological changes of wound healing granulation tissue, fibrosis and necrosis in diabetic Wistar rats on day 14. Materials and Methods: This study was a true experimental design with a post-test only control group design. The samples were divided into two groups, namely the diabetic model group given CM extract at 16 mL/kg BW dose for 14 days, and the diabetic model group given BR2 feed only for 14 days. Results: The results of Mann-Whitney U test showed p = 0.003 for granulation tissue, p = 0.411 for fibrosis and p = 1 for necrosis, with the level of significance was p<0.05. Conclusion: The CM extract at 16 mL/kg BW dose affects the histopathological description of granulation tissue which presents a better result than those in the control group but did not affect the features of fibrosis and necrosis in diabetic Wistar rats on day 14. Keywords: Channa Micropeltes, Diabetic wound, Fibrosis, Granulation tissue, Necrosis.

scholarly journals PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

2015 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Umi Kulsum ◽  
Ratnawati Hendari ◽  
Siti Chumaeroh
Keyword(s):  
Wound Healing ◽  
Ulcer Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Healing Process ◽  
Aloe Vera ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

scholarly journals EFEKTIVITAS PEMBERIAN EKSTRAK IKAN HARUAN (Channa striata) TERHADAP JUMLAH NEUTROFIL PADA PROSES PENYEMBUHAN ULKUS TRAUMATIKUS

DENTA ◽  
2019 ◽  
Vol 12 (2) ◽  
pp. 8
Author(s):  
Isidora Karsini ◽  
Farhana Nur Fadhila ◽  
Nafiah Nafiah
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Unsaturated Fat ◽  
Post Test ◽  
Channa Striata ◽  
The Mean

<p><strong><em>Background:</em></strong><em> </em><em>Channa striata contains important compounds in the process of tissue synthesis and important role in wound healing, such as albumin, zinc (Zn), copper (Cu), iron (Fe) and unsaturated fat acids. <strong>Purpose :</strong> To determine the effect of Channa striata extract to the amount of neutrophil in healing process of traumatic ulcer. <strong>Material and Method :</strong> The design of this study used randomized post test only control group design. 18 wistar rats were divided into 6 groups, i.e: K-1 (no treatment for 1 day), K-3 (no treatment for 3 days), K+1 (giving hyaluronic acid 0.2% for 1 day), K+3 (giving hyaluronic acis 0.2% for 3 days), P1 (giving Channa striata extract 100% for 1 day), P3 (giving Channa striata extract 100% for 3 days). <strong>Results :</strong> There was a decrease in the number of neutrophil in the treatment group. The mean and standart deviation of the number of neutrophil in the K-1 group (75,96±3,65), K-3 (50,76±2,04), K+1(72,96±2,97),K+3(45,06±2,83),P1(54,20±1,31),P3(32,50±,85). <strong>Conclusion :</strong> The using of Channa striata extract 100% for 3 days are the most effective in decreasing the amount of neutrophil in healing process of traumatic ulcer of Rattus Novergicus Strain Wistar.</em></p>

scholarly journals Effect of Photobiomodulation Therapy on Oxidative Stress Markers in Healing Dynamics of Diabetic Neuropathic Wounds in Wistar Rats

2021 ◽  
Author(s):  
Gagana Karkada ◽  
G. Arun Maiya ◽  
Praveen Arany ◽  
Mohandas Rao ◽  
Shalini Adiga ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Photobiomodulation Therapy ◽  
Control Group ◽  
Sciatic Nerve Crush ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Experimental Group

Abstract Background Prolonged and overlapping phases of wound healing in diabetes are mainly due to the redox imbalance resulting in the chronicity of the wound. Photobiomodulation therapy works on the principle of absorption of photon energy and its transduction into a biological response in the living tissue. It alleviates the cellular responses, thereby improving the mechanism of wound healing in diabetes. Objective To find out the effect of photobiomodulation therapy of dosage 4 J/cm2 in the healing dynamics of diabetic neuropathic wounds in Wistar rats and its relation with oxidative stress markers. Methodology Diabetes was induced using Streptozotocin of 60 mg/kg of body weight to eighteen female Wistar rats. Neuropathy was induced by the sciatic nerve crush injury followed by an excisional wound of 2 cm2 on the back of the animal. Experimental group animals were treated with dosage 4 J/cm2 of wavelength 655 and 808 nm, and control group animals were kept unirradiated. The biomechanical, histopathological, and biochemical changes were analysed in both groups. Results There was a reduction in mean wound healing time and an increased rate of wound contraction in the experimental group animals compared to its control group. The experimental group showed improved redox status, and histopathological findings revealed better proliferative cells, keratinisation, and epithelialization than un-irradiated controls. Conclusions Photobiomodulation therapy of dosage 4 J/cm2 enhanced the overall wound healing dynamics of the diabetes-induced neuropathic wound and optimised the oxidative status of the wound, thereby facilitating a faster healing process.

scholarly journals The effect of papaya leaf extract (Carica papaya L) on healing process of buccal traumatic ulcer in wistar rats

2019 ◽  
Vol 1 (1) ◽  
pp. 15
Author(s):  
Afryla Femilian ◽  
Dewi Agustina ◽  
Goeno Subagyo
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Buccal Mucosa ◽  
Wistar Rats ◽  
Carica Papaya ◽  
Healing Process ◽  
Herbal Medicines ◽  
Ethanolic Extract ◽  
Control Group ◽  
Wound Healing Process

Indonesians have been using herbal medicines for a long time to cure some illnesses. Carica papaya L is an example of an herb that contains papain enzymes, saponins, lysozymes, lipases, flavonoids, polyphenols and vitamin C. These  ingredients are believed to be beneficial for the wound healing process. The purpose of this study was to determine the effect of topical application of ethanolic extract of Carica papaya L to the healing process of rat mouth ulcer. Subjects were 32 Wistar rats divided into 2 groups (control group and treatment group), each group containing 16 rats. All subjects were made to suffer from ulcers using glacial acetyl acid applied for 40 seconds in the buccal mucosa. Treatment group was treated with papaya leaf ethanolic extract on ulcers twice a day using microbrush, while the ulcer in the control group was not treated with the extract. The ulcerated tissue was biopsied and stained with H&E. Observations were performed on the day 0, 3rd, 7th and 12th on HE slides. Data were observed by looking at three indicators of wound healing i.e. macrophage, angiogenesis and re-epithelisation. Number of macrophages and angiogenesis were analyzed using two-way ANOVA. Data of epithelial thickness were analyzed using Kruskal-Wallis test. The post hoc test in the treatment group and the control group on day 0 showed results of p = 1.00. On day 3, day 7 and day 12, the result of p was <0.05. In the treatment group on day 0 compared to the treatment group on the 3rd, 7th, 12th day, the results were p<0.05. In the treatment group on the 3rd day compared to the 7th and 12th days the results were p>0.05. The treatment group on the 7 day compared to the treatment group on the 12 day had p>0.05. In the control group, on day 0 compared to day 3, the results of p>0.05, while the control group day 0 with day 7, 12 had a result of p<0.05. The control group on the 3rd day was compared with the 7th day. The 12th also had a result of p<0.05. The comparison between the 7 day control groups showed p of >0.05. These data suggest that the papaya leaf ethanolic extract could accelerate the healing of oral ulcer on the buccal mucosa of wistar rats.

scholarly journals Expression of Vascular Endothelial Growth Factor (VEGF) and New Blood Vessels Formation on Wound Incision Post Ropivacaine Administration in Animal Model

2020 ◽  
Vol 5 (7) ◽  
pp. 829-836
Author(s):  
Andri Subiantoro ◽  
Arie Utariani ◽  
Imam Susilo
Keyword(s):  
Wound Healing ◽  
Endothelial Cells ◽  
Blood Vessels ◽  
Wistar Rats ◽  
Healing Process ◽  
Critical Factor ◽  
Histological Evaluation ◽  
Control Group ◽  
Wound Healing Process ◽  
Impaired Wound Healing

Surgical incision wounds causing clinical pain. Inadequate postoperative pain management resulting in impaired wound healing. A critical factor in wound healing is angiogenesis. VEGF is one of the important factors and regulators of angiogenesis. The aim of this study is to analyze the effect of infiltration ropivacaine on VEGF expression, and the formation of new blood vessels, which is very instrumental in the wound healing process. Methods: Twenty-four Wistar rats were divided into two groups, and then superficial-thickness excisional wounds were created. One was treated by ropivacaine infiltration, and the other was not given ropivacaine infiltration as control group. Each of the groups was divided into two subgroups. Each of the subgroups composed of 6 Wistar rats based on the period of termination: 3rd, 7th day after wounding. Histological evaluation was done to determine the presence of new small blood vessels and the expression of VEGF of capillary endothelial cells. The data collected is then analysed by SPSS. Results: The administration of ropivacaine infiltration on superficial-thickness excisional wounds improved wound healing characterized by neovascularization formed and changed of expression of VEGF within cytoplasm or surface of endothelial cells Conclusion: The administration of ropivacaine infiltration has a beneficial effect on the healing process of superficial Wistar rats.

scholarly journals Acceleration of fibroblast number and FGF-2 expression using Channa striata extract induction during wound healing process: in vivo studies in wistar rats

2016 ◽  
Vol 49 (3) ◽  
pp. 125
Author(s):  
Gunawan Oentaryo ◽  
Istiati Istiati ◽  
Pratiwi Soesilawati
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Tissue Growth ◽  
Control Group ◽  
Wound Healing Process ◽  
Lower Lip ◽  
Treatment Groups ◽  
Collagen Secretion ◽  
Channa Striata

Background: Wound healing is a biological process associated with tissue growth and regeneration. Wound healing process, is important to repair damaged tissue. Wound healing process consists of coagulation and hemostasis, inflammation, proliferation, as well as remodeling phases. The process can be accelerated by taking synthetic or non synthetic drugs. One of them is Channa striata extract. The extract contains albumin, copper, and zinc, which can be assumed to increase inflammatory cell infiltration, fibroblast proliferation, and collagen secretion. Purpose: This study aimed to reveal the effects of Channa striata extracts on fibroblast number and FGF-2 expression in mucosal wound healing process of the Wistar rats’ lower lip. Method: This research was a true laboratory experimental research with randomized post test only control group design. Samples of experiment were devided to experiment and control group that consist five samples each. Experimental group was treted with Channa striata extract and ethanol at concentration of 25%, 50%, and 100%. The fibroblast number and FGF-2 expresion were examined. Result: The number of fibroblasts in the treatment groups receiving Channa striata extract at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest number of fibroblasts was found on day 3 at the concentration of 100% (p<0.05). Similarly, FGF-2 expression in the treatment groups receiving Channa striata at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest expression of FGF-2 was found on day 3 at the concentration of 50% (p<0.05). Conclusion: Channa striata extract increased fibroblast number and FGF-2 expression in mucosa wound healing process.

scholarly journals Effectiveness Cream and Ointment of Spirulina platensis extract against amount of Fibroblas and Wound Area: Study on white rats whose skin is incised

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Bimby Irenesia ◽  
Renni Yuniarti ◽  
Endang Mahati
Keyword(s):  
Wound Healing ◽  
Spirulina Platensis ◽  
Wistar Rats ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design ◽  
Wound Area ◽  
White Rats

Spirulina platensishas been well-known as an abundant protein and phycocyanin. Spirulina platensis has the effect of anti-inflammatory, so it is widely used to apply cosmetics and biomedicine in the healing process of wounds. To prove that the administration of an extract ofSpirulina platensiscan increase the number of fibroblasts and accelerate the wound healing process in Wistar rats with an incision on their skin. This study used a randomizedpost-test only control group design. The skins of thirty (30) male Wistar rats were incised. This treatment was divided into 4 groups, namely the group that received the 0,1% cream (X1) of S.platensisextract, and the group that was administered with the 0,1% ointment (X2) of S.platensis extract; the negative control group was only treated by a saline solution (C1), and the positive control group was with the administration of Gentamycin 0,1% ointment (C2). The total numbers of fibroblasts were examined on day 14 and calculated using the method of tissue biopsy and hematoxylin-eosin (HE) staining. The wound was checked after 14-day Data analysis was performed with a one-wayANOVA test and was continued by Post Hoc Test LSD. The total numbers of fibroblasts on day 14 from the group of XI, X2, C1, and C2 were 15.00; 17,50; 3,83, 10,33 with p<0.001. The average size of the wounds on day 14 from the groups of X1, X2, C1, and C2 was 551,78; 435,76; 1795,95; 1193,57 with p<0.001, respectively. PostHoc Testresults on the total number of fibroblasts and extensive wound area showed significant differences between them. Administration of the 0.1% ointment of Spirulina platensisextract believed to potentially increasing the number of fibroblasts and accelerate the process of wound healing.

scholarly journals wound-healIng effect of polyguanIdIne-based hydrogel

2016 ◽  
Vol 1 (5) ◽  
pp. 117-120 ◽  
Author(s):  
Стельмах ◽  
Sergey Stelmakh ◽  
Бадмаев ◽  
Nikita Badmaev ◽  
Разуваева ◽  
...  
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Healing Process ◽  
Lower Amount ◽  
Control Group ◽  
Animal Group ◽  
Healing Effect ◽  
Healing Agent ◽  
Wound Healing Effect ◽  
Poly Ethylene

This article present the evaluation of polyhexamethyleneguanidine hydrochloride hydrogel influence on the course of wound healing processes. Synthesis of hydrogel is based on the interaction of amino-end groups of the polymer with carbonyl compounds. The presence of significant biocidal properties of the gel-forming polymer is allowed to consider the hydrogel as a wound healing agent. Since most wound healing agents for external use are made in the form of gels, ointments, poly-mer-based, it was decided to use the wound healing agent “Levomekol”, a gel which consists of poly (ethylene oxide) as a comparison drug. So, in terms of linear skin-muscle wound we established the pronounced wound-healing effect of a polyhexamethyleneguanidine hydrochloride hydrogel. The results of pathomorphological studies revealed that in animals of the experimental “hydrogel” group on the eighth day of the experiment, the wound was undergoing a process of rejection of the scab, the presence of mature granulation tissue and no exudate was also observed. In the comparison group, the healing process was more slow, on the eighth day of the experiment in the animal group we observed the presence of fluid under the eschar, and lower amount of mature granulation tissue. Tensiometry of the scar has shown that the strength of scar rupture in the “hydrogel” group requires 65% more force compared to the control group, while in the group of comparison this parameter made only 48%.

scholarly journals Obesity and abdominal wound healing in rats

2010 ◽  
Vol 25 (1) ◽  
pp. 86-92 ◽  
Author(s):  
Maria de Lourdes Pessole Biondo-Simões ◽  
Guilherme Roberto Zammar ◽  
Rodrigo dos Santos Fernandes ◽  
Rachel Biondo-Simões ◽  
Flavia Stica Ritzdorf de Mello ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Control Group ◽  
Average Weight ◽  
Standard Diet ◽  
Abdominal Wound ◽  
Calorie Diet ◽  
Collagen Density ◽  
The Difference

INTRODUCTION: Treatment for obesity essentially has to do with weight loss, which can be achieved through surgical procedures. Despite the considerable rise in the number of such procedures, the relationship between obesity and the healing process has not been totally clarified. PURPOSE: To investigate abdominal wound healing in obese Wistar rats on the seventh and fourteenth days following a laparotomy. METHODS: Thirty-six Wistar rats were randomly distributed into two groups, the control and experiment group. The control group were fed on either a standard diet for the species and the experiment group were put on a high calorie diet. After 116 days, all the animals were submitted to a laparotomy followed by laparorrhaphy. After euthanasia on the seventh or fourteenth day, fragments of the abdominal wound containing the scar were submitted to histopathological and tensiometric analysis. RESULTS: The average weight of the animals from the experiment group was higher than that of the control group (p<0.001). The difference in the resistance of the cutaneous scars was not significant. The aponeurotic scars were more resistant in the control group after seven days (p=0.011) and fourteen days (p=0.040). There was no difference in terms of intensity of the inflammatory reaction and the collagen density was similar in both groups. CONCLUSION: In rats, obesity lowered the resistance of the aponeurotic scars but not the skin scars. It did not interfere with the delayed inflammatory response and the collagen density.

scholarly journals Pengaruh Bone Marrow Mesenchymal Stem Cells Terhadap Sekresi VEGF pada Penyembuhan Luka Bakar Tikus

2018 ◽  
Vol 6 (3) ◽  
pp. 702
Author(s):  
Gusti Revilla
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Granulation Tissue ◽  
Wistar Rats ◽  
Healing Process ◽  
Control Group ◽  
Full Thickness ◽  
Post Test ◽  
Marrow Mesenchymal Stem Cells

Treatment for burns have been carried out in various ways, but have not provided satisfactory results, so that using stem cells is necessary. Administering Bone Marrow Mesenchymal Stem Cells (BM-MSCs) for healing, are by accelerating the formation of granulation tissue, however the effectiveness of the provision of is still under exploration. The objective of this study was to determine the effect of the administration of stem cells BM-MSCs on VEGF secretion on burn healing process in rats. This research was an experimental research with design the post-test only control, using 12 Wistar rats, divided into 2 groups: control (PBS), treatment (BM-MSCs), stem-cells subcutaneously administered dose of 2 x 106 cells / ml. Before that the treated rats anesthetized using xylazine and ketamine then burns are made on the dorsal with full thickness degrees. On day 3 and 7, blood were obtained to measure levels of VEGF was measured by ELISA. The results were analyzed by t-test. Levels of VEGF in day 3 and day 7 in the group given BM-MSC were dereased 12.875 pg/ml dan 23.917 pg/ml compared to the control group 61.667 pg/ml and 200.042 pg/ml and showed significant differences p= 0.012 and p=0.038. The conclusion of this study is the administration of BM-MSCs were the influence levels of VEGF which has into differentiate to cells that contribute to angiogenesis was can to granulation tissue. 

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