scholarly journals Phytochemical screening of Lycopersicum esculentum Mill. treated with seaweed liquid fertilizer

2018 ◽  
Vol 8 (6-s) ◽  
pp. 38-43
Author(s):  
M. Niranjanadevi ◽  
K. Sivakumar
Keyword(s):  
Gas Chromatography ◽  
Vitamin E ◽  
Functional Groups ◽  
Decanoic Acid ◽  
Phytochemical Analysis ◽  
Lycopersicum Esculentum ◽  
Liquid Fertilizer ◽  
Biological Studies ◽  
Phytochemical Compounds ◽  
Seaweed Liquid Fertilizer

Premise of the study: The objective of the work is to manitor the phytochemicals present in methanolic extract of Lycopersicum esculentum after treatment with different concentrations of seaweed liquid fertilizer (SLF). Methods: In this study SLF treated L.esculentum were characterized through gas chromatography and mass spectroscopy to identify the phytochemicals constituents and functional groups of the compounds. Results: Preliminary phytochemical analysis revealed the presence of alkaloids, phenols, carbohydrates, saponins, glycosides, flavonoids, terpinoids. Among the thirty nine phytochemical constituents octa decanoic acid, stigmasterol, hexa decanoic acid and Vitamin E are relatively present in higher yield. The active phytochemical compounds and their constituents were identified with gas chromatography and mass spectrometry. The retention time, percentage of area, molecular weight and chemical formula of phytochemical compounds were determined with help of NIST08 and WILEY8 libraries. The functional groups identified from the spectrum techniques are alcohol, aldehyde, iso cyanides, alkyl compound and chloro compounds. Accordingly hexadecanic acid, beta.-amyrin, gamma sisterol, octodecanic acid, phytol, stigmasterol, vitamin E, lupeol were derived from SLF treated i.e. compared to control plants. Conclusion: The results of this study offer a platform of using SLF treated L.esculentum has an alternative source for various biological studies and it can be used as functional and pharmaceutical purposes. Keywords: SLF, Lycopersicum esculentum, Phytochemical, GC-MS and FTIR

Gas chromatography: a mature technique with a bright future in phytochemical analysis

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
C Bicchi ◽  
C Cagliero ◽  
C Cordero ◽  
E Liberto ◽  
B Sgorbini ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Phytochemical Analysis ◽  
Bright Future

scholarly journals Phytochemical Screening and Biological Studies of Shilajit (Asphaltum)

2017 ◽  
Vol 9 (1) ◽  
pp. 15
Author(s):  
Shahid Aziz ◽  
Sidra Khaliq ◽  
Habib Ur-Rehman ◽  
Kh. Shakeel Ghani ◽  
Muhammad Irshad ◽  
...  
Keyword(s):  
Microbial Activity ◽  
Cardiac Glycosides ◽  
Reducing Sugars ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Biological Studies ◽  
Dead Plant ◽  
Micro Organisms

<p>Shilajit (asphaltum)  is produced  by the long term humification  of dead plant material  and organic vegetable matter  by different micro-organisms and has great potential for the treatment of a variety of human conditions.  This treatise reviews its origin, sources, chemical composition, biological  and  commercial importance. Phytochemical analysis was done by standard methods to evaluate  different Shilajit (asphaltum) classes of compounds in different samples of shilajit  which are responsible for their  biological activity.  Shilajit`s anti-microbial activity has been evaluated  against four different bacterial strains viz., <em>Escherichia coli, Psuedomonas aeuroginosa</em>, <em>Klebisella pneumonia</em> and <em>Staphylococcus aureus. </em>Phytochemical analysis illustrated  that shilajit contains  terpenoids, cardiac glycosides, saponins and reducing sugars. Surprisingly,  some classes of compounds are absent in shilajit  viz., alkaloids, flavonoids, tannins and anthraquinones. . Shilajit showed no response towards halophytic bacteria and  negligible activity was shown towards other strains of bacteria. Since   anti-microbial activity is based on environmental factors  its activity varied  between locations.</p>

Effect of seaweed liquid fertilizer on yield and quality of Capsicum annum L.

2019 ◽  
Vol 39 (5) ◽  
pp. 406-410 ◽  
Author(s):  
S. Vijayakumar ◽  
S. Durgadevi ◽  
P. Arulmozhi ◽  
S. Rajalakshmi ◽  
T. Gopalakrishnan ◽  
...  
Keyword(s):  
Liquid Fertilizer ◽  
Capsicum Annum ◽  
Yield And Quality ◽  
Seaweed Liquid Fertilizer

Quantitative Determination of Vitamin E Isomers using Gas Chromatography, High Performance Liquid Chromatography and High Performance Thin Layer Chromatography

2017 ◽  
Vol 54 (3) ◽  
pp. 294
Author(s):  
Hari Ramakrishnan K. ◽  
Janaky Ranjithkumar
Keyword(s):  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin E ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Highly Sensitive ◽  
Layer Chromatography

Vitamin E, the fat soluble vitamin is present naturally in some foods and added in food supplements, nutraceuticals etc due to its vital biological function as an antioxidant. Various methods are available for the analysis of vitamin E. Especially High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) are exclusively used for the quantitative evaluation of vitamin E, which has also identified the four different isomeric forms of this vitamin. The rate of losses of this vitamin during food processing and analysis, in addition to their transient dynamics, presents complexities in developing a highly sensitive procedure for their separations. Though effective, HPLC instrument is expensive and comparatively cumbersome. In this prospective, the study was to evaluate the usefulness of High Performance Thin Layer Chromatography (HPTLC) in the analysis of vitamin E. There are methods available using Thin Layer Chromatography for its analysis, but they are not sensitive enough to identify the isomeric forms of vitamin E. In this HPTLC method, the different isomeric forms of vitamin E - α, β, γ and δ were identified. This technique shall be considered as an alternative to the other methods such as HPLC and GC.

Comparison of Emmerie-Engel Method and Gas Chromatographic Method for Determination of Supplemental α-Tocopheryl Acetate in Feed Concentrates

1987 ◽  
Vol 70 (3) ◽  
pp. 417-419
Author(s):  
Michael P Labadie ◽  
Charles E Boufford
Keyword(s):  
Gas Chromatography ◽  
Vitamin E ◽  
Coefficient Of Variation ◽  
Chromatographic Method ◽  
Tocopheryl Acetate ◽  
High Potency ◽  
Gc Analysis ◽  
Gas Chromatographic Method ◽  
Official Procedure

Abstract The determination of supplemental a-tocopheryl acetate in high potency vitamin E powders and oils was compared using the Emmerie- Engel method and gas chromatography (GC). The Emmerie-Engel reaction requires saponification, extraction of the saponiflable fracaon, and quantitation by colorimetry. GC analysis requires only an extraction and/or dilution before quantitation. These are represented essentially by AOAC methods 43.147-43.151 (colorimetry) and 43.152-43.159 (GC) for high potency vitamin E concentrates. Each method was statistically evaluated for precision and sample-to-sample reproducibility. Each Emmerie-Engel value was divided by the GC value obtained for the same sample; an average of 1.049 with a coefficient of variation of 2.89% was obtained. It was concluded that (he GC procedure was superior to the Emmerie-Engel method, and ahould be the official procedure for determination of supplemental a-tocopheryl acetate in feed concentrates.

CHEMICAL AND BIOLOGICAL STUDIES RELATED TO THE METABOLIC FUNCTION OF VITAMIN E

1949 ◽  
Vol 52 (3 Vitamin E) ◽  
pp. 188-194 ◽  
Author(s):  
Paul D. Boyer ◽  
M. Rabinovitz ◽  
E. Liebe
Keyword(s):  
Vitamin E ◽  
Metabolic Function ◽  
Biological Studies

scholarly journals In Vitro Bioactivity of Methanolic Extracts from Amphipterygium adstringens (Schltdl.) Schiede ex Standl., Chenopodium ambrosioides L., Cirsium mexicanum DC., Eryngium carlinae F. Delaroche, and Pithecellobium dulce (Roxb.) Benth. Used in Traditional Medicine in Mexico

2018 ◽  
Vol 2018 ◽  
pp. 1-11
Author(s):  
Peter Knauth ◽  
Gustavo J. Acevedo-Hernández ◽  
M. Eduardo Cano ◽  
Melesio Gutiérrez-Lomelí ◽  
Zaira López
Keyword(s):  
Bark Extract ◽  
Phytochemical Analysis ◽  
Potential Candidate ◽  
Chenopodium Ambrosioides ◽  
Bacterial Strains ◽  
Fruit Extract ◽  
Methanolic Extracts ◽  
Biological Studies ◽  
First Time

Seven out of eight methanolic extracts from five plants native to Mexico were inactive against ten bacterial strains of clinical interest. The fruit extract of Chenopodium ambrosioides inhibited the bacteria Enterococcus faecalis (MIC = 4375 μg/ml), Escherichia coli (MIC = 1094 μg/ml), and Salmonella typhimurium (MIC = 137 μg/ml). The fruit extract of C. ambrosioides was with CC50 = 45 μg/ml most cytotoxic against the cell-line Caco-2, followed by the leaf extract from Pithecellobium dulce (CC50 = 126 μg/ml); interestingly, leaves of C. ambrosioides (CC50 = 563 μg/ml) and bark of P. dulce (CC50 = 347 μg/ml) extracts were much less cytotoxic. We describe for the first time the cytotoxic effect from extracts of the aerial parts and the flowers of Cirsium mexicanum (CC50 = 323 μg/ml and CC50 = 250 μg/ml, resp.). Phytochemical analysis demonstrated for both extracts high tannin and saponin and low flavonoid content, while terpenoids were found in the flowers. For the first time we report a cytotoxicological study on an extract of Eryngium carlinae (CC50 = 356 μg/ml) and likewise the bark extract from Amphipterygium adstringens (CC50 = 342 μg/ml). In conclusion the fruit extract of C. ambrosioides is a potential candidate for further biological studies.

Phytochemical Screening, Isolation, and Structure Elucidation of the Radish (Raphanus sativus Linn.) Bulb Ethanolic Extract Using Gas Chromatography- Mass Spectrometry (GC-MS)

10.17158/233 ◽  
2012 ◽  
Vol 18 (1) ◽  
Author(s):  
Ma. Eva C. San Juan ◽  
Ramchand S. Jumala ◽  
Karen Hope G. Niasca
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Raphanus Sativus ◽  
Ethanolic Extract ◽  
Bioactive Molecules ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Chromatography Mass Spectrometry ◽  
Long Chain

Nowadays, plant is being used as a biofuel, a probable source for a pesticide and it is valued for its pharmacological effects. Hence, the researchers engaged in the scientific approach to provide explanation to these claims. The study focused on the phytochemical screening of the ethanolic extract of radish bulbs (Raphanus sativus). It further dealt on the isolation and determination of the molecular masses to provide probable structures and associated molecular properties for its constituents.<br />Alkaloids, flavonoids, saponins and tannins were analyzed. Further, this effort involved analysis through Thin Layer Chromatography (TLC) using the mobile phase of Toluene: Chloroform (9:11) and Gas Chromatography- Mass Spectrometry (GC-MS) to analyze the first spot (Retention Factor= 0.667).<br />Phytochemical analysis showed no tannins. Using TLC, it revealed two spots having Rf values of 0.667 and 0.507, respectively. Using GC-MS method, 24 different molecules were isolated and analyzed of which only 23 were given probable structures. The 23 molecules constituted 11 long-chain alkanes, 4 long-chain alkyl esters, 2 aromatic ketones, a benzyl halide, an aryl ether, a quinone, an aromatic aldehyde, a long chain aromatic alkane, and a long-chain alkyl nitrile. With this finding, the bulb extract may be a good source of potential biofuel (mono-alkyl ester), UV A, B and C blockers in the form of diphenyl-ketone, oviposition attractants (long-chain alkanes) and also a source of bioactive molecules such as estragole which shows antispasmodic effect, methyl palmitate with immunosuppressive effect in semi-allografts and hepatoprotective activity in rats, and an analogue of the antidepressant alfetamine.

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