scholarly journals Formulasi Krim Tabir Surya Ekstrak Etanol Daun Kersen (Muntingia calabura L.) untuk Kesehatan Kulit

2018 ◽  
Vol 28 (4) ◽  
pp. 263-270
Author(s):  
Anita Dwi Puspitasari ◽  
Dewi Andini Kunti Mulangsri ◽  
Herlina Herlina
Keyword(s):  
Ethanolic Extract ◽  
Sun Exposure ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Physical Chemical ◽  
Rotary Evaporator ◽  
Maximum Protection ◽  
Physical And Chemical ◽  
Muntingia Calabura

Sunlight has many health benefits, but high sun exposure can also cause skin problems ranging from redness, inflammation, and the worst is triggering the appearance of skin cancer. One way to protect the skin from the sun is by using a sunscreen. Kersen Leaf (Muntingia calabura L.) has a high total flavonoids and total phenolic so that it can be used as a natural active ingredient for making sunscreen creams. The purpose of this study was to formulate and evaluate sunscreen cream preparations with variations in the concentration of ethanol extract of Kersen Leaf  Muntingia calabura L.) and to know the SPF value. Extract of kersen leaves (Muntingia calabura L.) using the maceration method with 70% ethanol. The extract was concentrated using a rotary evaporator. Four formulas were made with variations in the concentration of ethanol extract of kersen leaves. Formula 1 (cream base) without ethanolic extract of kersen leaves; formula 2 with ethanol extract of 1 gram kersen leaves; formula 3 with ethanol extract of 2 grams kersen leaves and formula 4 with ethanol extract of 3 grams kersen leaves. The four formulas were tested for physical, chemical and SPF values. Testing the SPF value using spectrophotometric method. For sticky power does not meet standard because it is less than 4 seconds. The Formula 1 SPF value is 0.1149; formula 2 is 7.6574 (extra protection); formula 3 is 13.7847 (maximum protection); and formula 4 is 19.0871 (ultra protection). The greater the concentration of ethanol extract of kersen leaves the greater the SPF value. From the results of the study, it was found that the four formulas fulfilled the requirements of physical and chemical characteristics, namely organoleptic, homogeneity, distribution, and viscosity, and had a significant SPF values, namely formula 2, 3 and 4. Abstrak Sinar matahari memiliki banyak manfaat bagi kesehatan, namun paparan sinar matahari yang tinggi juga dapat menyebabkan masalah kulit mulai dari kemerahan, peradangan, dan yang paling buruk adalah memicu munculnya kanker kulit. Salah satu cara untuk melindungi kulit dari sinar matahari yaitu dengan menggunakan tabir surya. Daun kersen (Muntingia calabura L.) mempunyai kandungan flavonoid total dan fenolik total yang tinggi sehingga dapat dimanfaatkan sebagai bahan aktif alami untuk pembuatan krim tabir surya. Tujuan penelitian ini untuk melakukan formulasi dan evaluasi sediaan krim tabir surya dengan variasi konsentrasi ekstrak etanol daun kersen (Muntingia calabura L.) serta mengetahui nilai SPF-nya. Ekstraksi daun kersen menggunakan metode maserasi dengan pelarut etanol 70%. Ekstrak dipekatkan menggunakan rotary evaporator. Dibuat empat formula dengan variasi konsentrasi ekstrak etanol daun kersen. Formula 1 (basis krim) tanpa ekstrak etanol daun kersen; formula 2 dengan ekstrak etanol daun kersen 1 gram; formula 3 dengan ekstrak etanol daun kersen 2 gram; dan formula 4 dengan ekstrak etanol daun kersen 3 gram. Keempat formula diuji karakteristik sifat fisika, kimia dan nilai SPFnya. Pengujian nilai SPF menggunakan metode spektrofotometri. Untuk daya lekat belum memenuhi standar karena kurang dari empat detik. Nilai SPF Formula 1 sebesar 0,1149; formula 2 sebesar 7,6574 (proteksi ekstra); formula 3 sebesar 13,7847 (proteksi maksimal); dan formula 4 sebesar 19,0871 (proteksi ultra). Semakin besar konsentrasi ekstrak etanol daun kersen semakin besar nilai SPF-nya. Dari hasil penelitian diperoleh bahwa keempat formula memenuhi persyaratan karakteristik sifat fisika dan kimia yaitu organoleptis, homogenitas, daya sebar pH, dan viskositas, serta memiliki nilai SPF yang bermakna yaitu formula 2, 3, dan 4.

scholarly journals PENENTUAN NILAI SPF EKSTRAK DAN LOSIO TABIR SURYA EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) DENGAN METODE SPEKTROFOTOMETRI UV-VIS

2019 ◽  
Vol 1 (3) ◽  
pp. 189-202
Author(s):  
Erni Widyawati ◽  
Nurista Dida Ayuningtyas ◽  
Agustina Putri Pitarisa
Keyword(s):  
Skin Cancer ◽  
Spectrophotometric Method ◽  
Sun Exposure ◽  
Ethanol Extract ◽  
Premature Aging ◽  
Total Flavonoids ◽  
Active Ingredients ◽  
Maximum Protection ◽  
Muntingia Calabura

Excessive and prolonged sun exposure can cause erythema and sunburn, premature aging and skin cancer. Kersen leaves contain total flavonoids and phenolics which can be used as active ingredients for making sunscreen lotion. This study was to determine the SPF value, the value of erythema transmission (% Te) and the value of pigmentation Transmission (% Tp) from extracts and lotions of kersen ethanol extract using the Uv-Vis spectrophotometric method. Absorbance increases at wavelengths of 290-400 nm at intervals of 5 nm. The results showed SPF extract values ​​with a composition of 500 ppm, 1000 ppm, and 2000 ppm combined - 12 were 12.15 (maximum ability); 17,29 (ultra protection); and 22.01 (ultra protection). % Te value 0.54; 0.16 and 0.05 all include the total block category,% value Tp 2.50; 1.30 and 0.49 (total blocks). Whereas for losio with a concentration of 0.5%, 1% and 2% respectively the SPF value produced is 3.61 (minimum protection); 8.18 (maximum protection) and 10.13 (maximum protection). % Te value 4.29 (extra protection); 1.58 (extra protection) and 0.99 (maximum protection). % value Tp 9.30; 4.22 and 2.80 are all included in the total block category. Keywords :  kersen leaves, sunscreen lotion, SPF, erythema transmission, pigmentation transmission

scholarly journals Evaluasi Karakteristik Fisika Kimia dan Nilai SPF Sediaan Gel Tabir Surya Ekstrak Etanol Daun Kersen (Muntingia calabura L)

2019 ◽  
Vol 5 (2) ◽  
Author(s):  
Anita Dwi Puspitasari ◽  
Dyah Ayu Setyowati
Keyword(s):  
Health Benefits ◽  
Ethanol Extract ◽  
Chemical Characteristics ◽  
The Sun ◽  
Basic Ingredient ◽  
Physical Chemical ◽  
Rotary Evaporator ◽  
Gel Preparation ◽  
Physical And Chemical ◽  
Muntingia Calabura

Selain memiliki banyak manfaat bagi kesehatan, sinar matahari juga dapat menyebabkan masalah kulit mulai dari yang ringan sampai yang berat. Salah satu cara untuk melindungi kulit dari sinar matahari yanitu dengan menggunakan tabir surya. Salah satu sediaan tabir surya yaitu berupa gel. Daun kersen (Muntingia calabura L ) mempunyai kandungan flavonoid total dan fenolik total yang tinggi sehingga dapat dimanfaatkan sebagan bahan dasar pembuatan gel tabir surya. Tujuan penelitian ini adalah untuk mengetahui karakteristik fisika, kimia dan nilai SPF sediaan gel tabir surya ekstrak etanol daun kersen (Muntingia calabura). Ekstraksi daun kersen (Muntingia calabura L) menggunakan metode maserasi dengan pelarut etanol 70% dan dipekatkan menggunakan rotary evaporator. Gel tabir surya ekstak etanol daun kersen dibuat menggunakan basis HPMC. Dibuat 4 formula dengan perbedaan pada konsentrasi ekstrak etanol daun kersen, yaitu 0, 1, 2, dan 3 gram. Keempat formula gel ekstrak etanol daun kersen diuji karakteristik fisika, kimia dan nilai SPFnya menggunakan metode Mansur. Keempat formula gel esktrak etanol daun kersen memenuhi semua persyaratan karakteristik sifat fisika dan kimia yaitu organoleptis, homogenitas, daya sebar, daya lekat, pH, dan viskositas. Nilai SPF untuk formula 1, 2, 3, dan 4 yaitu sebesar 0,38; 7,17; 11,15 dan 18,92. Kata kunci: gel, muntingia calabura, SPF, tabir surya Besides having many health benefits, sunlight can also cause skin problems ranging from mild to severe. One way to protect the skin from the sun is by using sunscreen. One of the sunscreen preparations is gel. Kersen leaves (Muntingia calabura L) have a high phenolic and flavonoids which can be used as a basic ingredient in making sunscreen gel. The purpose of this study was to determine the physical, chemical and SPF values of the gel preparation of sunscreen ethanol extract of kersen leaves (Muntingia calabura). Muntingia calabura L. leaf extraction using maceration method with 70% ethanol solvent and concentrated using rotary evaporator. Sunscreen gel extracts from kersen leaf ethanol made using HPMC base. Four formulas were made with differences in the concentration of kersen leaves ethanol extract, namely 0, 1, 2 and 3 grams. The four gel formulas of kersen leaf ethanol extract were tested for physical, chemical and SPF values using the Mansur method. The four gel formulas of kersen leaf ethanol extract fulfilled all the requirements of physical and chemical characteristics, namely organoleptic, homogeneity, dispersion, stickiness, pH, and viscosity. SPF values for formulas 1, 2, 3 and 4 are 0.38; 7.17; 11,15 and 18,92. Keywords: gel, muntingia calabura, SPF, sunscreen

Antioxidant and antimicrobial capacities of ethanolic extract of Pergularia daemia leaves: a possible substitute in diabetic management

2016 ◽  
Vol 13 (3) ◽  
Author(s):  
Joseph Adusei Sarkodie ◽  
Sylvia Afriyie Squire ◽  
Emelia Oppong Bekoe ◽  
Charles Yaw Fosu Domozoro ◽  
Irene Awo Kretchy ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Colorimetric Assay ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Total Flavonoids Content

Abstract: The leaves of: The total phenolic content, total flavonoids content, radical scavenging activity and reducing power assays were estimated using Folin–Ciocalteu method, aluminium chloride colorimetric assay, Fe: The results showed that: These findings justify the folkloric use of

scholarly journals Antioxidant Activity Analysis and Standardization of Parkia speciosa (Petai) Pods Ethanol Extract

2019 ◽  
Vol 4 (1) ◽  
pp. 5 ◽  
Author(s):  
Najma Annuria Fithri ◽  
Fitrya Fitrya ◽  
Tia Shabrina ◽  
Diva Yulanri
Keyword(s):  
Antioxidant Activity ◽  
Water Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Structural Stabilization ◽  
Dpph Method ◽  
Research Outcome ◽  
Phenolic And Flavonoid

Parkia speciosa (petai) pods are rarely used and considered as waste despite their phenolic and flavonoid content. Phenolic and flavonoid content in plants are known to exhibit antioxidant activity. Antioxidants are known to potentially act agains radical and mediate the damage by structural stabilization. This research was conducted to analyze the antioxidant potential of petai pods using DPPH method. Analysis showed the IC50 of petai pods ethanolic extract was 75.72 ppm, which indicate strong acting antioxidant. Standardization of extracts were determined to manage the quality of extract, including the analysis of density, water content, ash values, and quantification of total phenolic and flavonoid content. Research outcome indicate the extract retain its quality during storage and use which confrmed to standardization requirements of water content and ash values. Total phenolics and flavonoid obtained were 272.45 mg/gGAE and 243.2029 mg/gQE respectively. Based from this research, petai pods ethanolic extract present strong antioxidant activity which was contributed by high existing content of flavonoid and phenolic compounds.

scholarly journals Enzyme inhibitory, antioxidant, and antibacterial activities of ethanol fruit extract of Muntingia calabura Linn

2020 ◽  
Vol 9 (4) ◽  
pp. 346-354
Author(s):  
Adelina Simamora ◽  
Adit Widodo Santoso ◽  
Ika Rahayu ◽  
Kris Herawan Timotius
Keyword(s):  
Dna Damage ◽  
Xanthine Oxidase ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Antibacterial Activities ◽  
In Vitro Assays ◽  
Total Phenolic ◽  
Fruit Extract ◽  
Muntingia Calabura

Introduction: Muntingia calabura is used for many medicinal advantages. So far, limited study has been done for the bioactivities of M.calabura fruit. The study aimed to investigate the enzyme inhibitory, antioxidant, and antibacterial activities of M.calabura fruit. Methods: Ethanol extract of M.calabura fruit was tested for its inhibitory enzyme activities against key enzymes linked to human pathologies, such as diabetes (α-glucosidase and α-amylase), hyperuricemia (xanthine oxidase), and obesity (lipase). The antioxidant properties were investigated using different in vitro assays (DPPH, CUPRAC, reducing power, phosphomolybdenum, metal chelating and DNA-Damage protection assays). The fruit was also evaluated for its antibacterial activity against several gram positive and negative bacteria. Results: The total phenolic and flavonoid contents of the extract were 10.85 mgGAE/g and 3.30 mg QE/g, respectively. The fruit extract showed good inhibition against α-glucosidase and α-amylase (IC50 16.74 and 46.49 µg/ml, respectively), with activities stronger than acarbose (100.38 and 152.46 µg/ml, respectively). It exhibited weak inhibitory activity against xanthine oxidase (IC50 0.91 mg/ml) and lipase (IC50 16.48 mg/ml), weaker than the references used for respective test (IC50 allopurinol 5.31 µg/ml and orlistat 0.17 µg/ml). The extract showed antibacterial activities againts Chromobacterium violaceum, Staphylococcus aureus, Streptococcus mutans, Staphylococcus epidermidis, and Escherichia coli. The ethanol extract showed weaker antioxidant activities, when compared to ascorbic acid and BHT. However, the extract was able to protect DNA-damage. Conclusions: The study concludes that M. calabura fruit exhibits antioxidant, antibacterial, and enzyme inhibitory properties, thus can be a good source for pharmacological uses.

scholarly journals Determination of phenolic contents and antioxidant activity test of ethanol extract of Sirih merah (Piper crocatum Ruiz & Pav.) leaves using the DPPH method

2021 ◽  
Vol 13 (2) ◽  
pp. 137-142
Author(s):  
Kasta Gurning ◽  
◽  
Sifikal Lumbangaol ◽  
Risanti F. R. Situmorang ◽  
Saronom Silaban ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Extraction Process ◽  
Total Phenolic ◽  
Betel Leaf ◽  
Dpph Method

The research objectives were to identify the secondary metabolite components, total phenolic content and determine the antioxidant activity of the ethanol extract of red betel leaf (Piper crocatum Ruiz & Pav.). The extraction process was carried out by materation using ethanol as a solvent. Determination of total phenolic content was carried out colorimetrically with Folin-Ciocalteu reagent measured at a maximum wavelength of 765 nm. Determination of antioxidant activity using the DPPH method measured by spectrophotometry at a maximum wavelength of 517 nm. The results of phytochemical screening of the ethanolic extract of red betel leaf contain secondary metabolites, including flavonoid, phenolic, tannin, alkaloids, steroids, and triterpenoids. The total phenolic content of the red betel leaf ethanol extract was 0.949±0.003 mg GAE/g d.w. and has antioxidant activity (IC50) 84,656 including strong category as an antioxidant. Keywords: Piper crocatum Ruiz & Pav., Antioxidant, Ethanol extract, Folin-Ciocalteu and DPPH

scholarly journals Anti-Melanogenic Effect of Ethanolic Extract of Sorghum bicolor on IBMX–Induced Melanogenesis in B16/F10 Melanoma Cells

Nutrients ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 832 ◽  
Author(s):  
Hye Ju Han ◽  
Seon Kyeong Park ◽  
Jin Yong Kang ◽  
Jong Min Kim ◽  
Seul Ki Yoo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Sorghum Bicolor ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Melanoma Cells ◽  
Total Phenolic ◽  
Skin Whitening

To evaluate possibility as a skin whitening agent of Sorghum bicolor (S. bicolor), its antioxidant activity and anti-melanogenic effect on 3-isobutyl-1-methylxanthine (IBMX)-induced melanogenesis in B16/F10 melanoma cells were investigated. The result of total phenolic contents (TPC) indicated that 60% ethanol extract of S. bicolor (ESB) has the highest contents than other ethanol extracts. Antioxidant activity was evaluated using the 2,2’-azino-bis-(3-ethylbenzothiazolin-6-sulfonic acid) diammonium salt (ABTS)/1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activities and malondialdehyde (MDA) inhibitory effect. These results showed ESB has significant antioxidant activities. Inhibitory effect against tyrosinase was also assessed using L-tyrosine (IC50 value = 89.25 μg/mL) and 3,4-dihydroxy-L-phenylalanine (L-DOPA) as substrates. In addition, ESB treatment effectively inhibited melanin production in IBMX-induced B16/F10 melanoma cells. To confirm the mechanism on anti-melanogenic effect of ESB, we examined melanogenesis-related proteins. ESB downregulated melanogenesis by decreasing expression of microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase-related protein (TRP)-1. Finally, 9-hydroxyoctadecadienoic acid (9-HODE), 1,3-O-dicaffeoylglycerol and tricin as the main compounds of ESB were analyzed using the ultra-performance liquid chromatography-ion mobility separation-quadrupole time of flight/tandem mass spectrometry (UPLC-IMS-QTOF/MS2). These findings suggest that ESB may have physiological potential to be used skin whitening material.

scholarly journals The antimicrobial activity of Prunella vulgaris extracts

2015 ◽  
Vol 61 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Mohaddese Mahboubi ◽  
Atefeh Mahboubi ◽  
Nastaran Kazempour
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Extract ◽  
Total Phenolic Content ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Prunella Vulgaris ◽  
Ethanol Extracts

Summary Prunella vulgaris ( Labiatae family) or self-heal is traditionally used for different ailments such as eye pain and inflammation, headache, dizziness, sore throat and wound healing. Total phenolic and total flavonoid contents of extracts (methanol, ethanol and aqueous) were determined by a spectrophotometer. The antimicrobial activity was evaluated by micro broth dilution assay. The total phenolic content of P. vulgaris extracts were higher in aqueous extract (156.5 mg GAC/g) followed by ethanol extract and methanol extract. The TFC content of P. vulgaris methanol extract (82.8 mg QE/g) was higher than ethanol extract (22.7 mg QE/g) and aqueous extract (16.2 mg QE/g). The antimicrobial activity of methanol or ethanol extracts was higher than aqueous extract from P. vulgaris. The sensitivity of microorganisms to different extracts is related to type of pathogens. There is no positive relation between total phenolic content and its antimicrobial activity. Prunella vulgaris ethanolic extract as a source of phenolic and flavonoid contents can be used as an antimicrobial agent.

scholarly journals KORELASI KANDUNGAN FENOLIK DAN AKTIVITAS ANTIOKSIDAN DAUN JAMBU METE

Biomedika ◽  
2011 ◽  
Vol 3 (2) ◽  
Author(s):  
Ika Trisharyanti Dian Kusumowati ◽  
Rosita Melannisa ◽  
Kartikaning Ratri
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Anacardium Occidentale ◽  
Total Phenolic ◽  
Positive Correlation

The ethanolic extract of Anacardium occidentale L. leaves contains phenolic compounds,  avonoids, alkaloids, saponins, tannins, and steroids that could have antioxidant activity. The aim of this research was to determine the correlation between the antioxidant activity and the phenolic compounds of the ethanol extract of Anacardium occidentale L. leaf. The DPPH methode was used to determine the antioxidant activity. The total phenolic determined using Folin-Ciocalteu reagent. There was a positive correlation between antioxidant activity and total phenolic content in the ethanolic extract of cashew leaf with correlation coef cient R2 = 0.5888.Keywords: Anacardium occidentale L., antioxidant, phenolic compounds

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