scholarly journals Physiological and Pathological Alterations in the Mammary Gland of Holstein Cows during Transition Period

2020 ◽  
Vol 48 ◽  
Author(s):  
Jean Silva Ramos ◽  
Karina Medici Madureira ◽  
Vinicius Alvim Passos Baldacim ◽  
Cynthia Pereira Da Costa e Silva ◽  
Mailson Rennan Borges Dias ◽  
...  
Keyword(s):  
Blood Flow ◽  
Mammary Gland ◽  
Physical Examination ◽  
Transition Period ◽  
Bovine Mastitis ◽  
Subclinical Mastitis ◽  
Clinical Findings ◽  
Pathological Changes ◽  
California Mastitis Test ◽  
Prophylactic Measures

Background: Physiological and immunological adaptations occurred in peripartum period may be responsible for susceptibility to intramammary infections during colostrogenesis. Increase in blood flow and hormones cause physiological edema by accumulation of lymphatic fluid in interstitial space. Specific composition of mammary secretion increases the susceptibility to mastitis. So, the study of physical, cellular and microbiological changes in the mammary gland in this period are fundamental for understanding, diagnosing and adopting prophylactic measures for bovine mastitis. This study aimed to characterize physiological and pathological changes that occur in the mammary gland during transition period.Materials, Methods & Results: Thirteen cows, in second to fourth lactation were followed from 3 weeks pre-calving to 3 weeks post-calving. Cows were submitted weekly to physical examination of mammary gland, strip cup test, California Mastitis Test (CMT), Microscopic Somatic Cell Count (MSCC), and bacteriological examination of mammary secretion. Mammary quarters were divided into two groups: BAC-: samples that did not present bacteriological growth throughout transition period; BAC+: samples that presented bacteriological growth in at least two isolates of the same bacterial genus. Regarding physical examination of mammary gland, the parameters localized or generalized alteration in volume, asymmetry, reddish color, reduced skin elasticity, increased temperature, and swollen consistency presented higher frequencies in the BAC- group. On the other hand, infection in mammary quarters of cows from BAC+ group promoted a decrease in frequencies of increased volume, decreased elasticity, and elevated temperature in the mammary gland, in addition to a higher positivity to CMT and higher cellularity. Overall rate of intramammary infection was 25.82%, with non-aureus Staphylococci (NAS) being the predominant agent (69.2%). Maximum peak of bacterial isolates occurred immediately after calving (33.3%). Differences were observed between groups (P = 0.021) for CMT, showing a higher frequency of positive tests in BAC+ group (36.5%) compared to BAC- group (18%). MSCC varied between weeks (P = 0.001), groups (P = 0.001), and between the interaction of the two variables (P = 0.019). Highest median value of MSCC was observed at calving (1.5 × 106 cells/mL), being different (P = 0.001) from the +1, +2, and +3 weeks and the lowest median value of MSCC (0.08×106 cells/mL) was observed in +3 compared to previous weeks (P < 0.05).Discussion: NAS was predominantly isolated in mammary secretions of cows during peripartum, it is commonly found in the skin and canal of the teat and are associated with subclinical mastitis. Peak of mammary infection was observed on calving, probably by the cortisol peak resulting in a stress leucogram and decrease in the functional activity of immune cells. Changes observed in the physical examination of mammary gland of cows from BAC- group are compatible with physiological edema caused by circulatory changes that occurs with the proximity of calving, increasing blood flow necessary for production of colostrum. Alterations in the mammary quarters of cows from BAC+ group may be associated with functional loss of mammary parenchyma or persistent infectious process, characterizing clinical findings of pathological changes during transition period. Mammary quarters of the BAC+ group presented higher percentages of positive reactions to CMT in weeks +1 and +2, and higher MSCC values and are associated with infection of the mammary gland.

Variations in the levels of acute-phase proteins and lactoferrin in serum and milk during bovine subclinical mastitis

2021 ◽  
pp. 1-5
Author(s):  
Aarsha Raj ◽  
Vinodkumar Kulangara ◽  
Tresamol P. Vareed ◽  
Deepa P. Melepat ◽  
Latha Chattothayil ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Solid Phase ◽  
Bovine Mastitis ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Reactive Protein ◽  
Amyloid A ◽  
California Mastitis Test ◽  
Bacterial Organism

Abstract Variations in the levels of acute phase proteins and lactoferrin in serum and milk for diagnosis of subclinical mastitis in dairy cows are described in this research paper. Milking animals from two organized dairy farms in Kerala, India, were screened by California Mastitis Test (CMT), Electrical Conductivity test (EC) and Somatic Cell Count (SCC) test to identify animals affected with sub clinical mastitis (SCM). The concentrations of acute phase proteins (APP) Haptoglobin (Hp), C- reactive protein (CRP), Albumin, Lactoferrin (Lf) and α- 1 acid glycoprotein (AGP) in milk and Hp, Albumin, Serum Amyloid A (SAA) and CRP in the serum of 40 normal cows and 40 cows affected with sub clinical mastitis were assessed. Solid phase ELISA was employed for assessment of all parameters except the albumin levels, for which spectrophotometry was used. The values of Hp in milk; and SAA, AGP and Lf in serum, were significantly elevated in the group with sub clinical mastitis. Such variations were found to be independent of the specific bacterial organism causing the disease. These results show that significant variations exist in the levels of acute phase proteins Hp, AGP and Lf in milk, and SAA in serum of animals affected with subclinical bovine mastitis that are not affected by specific bacterial etiology.

scholarly journals Risk factors associated with the occurrence of multiresistant Staphylococcus spp. isolated from bovine subclinical mastitis in northern Brazil

2022 ◽  
Vol 43 (2) ◽  
pp. 901-910
Author(s):  
Lilian Bernardina Ferreira ◽  
◽  
Larissa de Freitas Santiago Israel ◽  
Renata Fernandes Rabello ◽  
Guilherme Nunes de Souza ◽  
...  
Keyword(s):  
Risk Factors ◽  
Multidrug Resistance ◽  
Phenotypic Expression ◽  
Bovine Mastitis ◽  
Subclinical Mastitis ◽  
Isolation And Identification ◽  
Phenotypic Profile ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Staphylococcus Spp

Staphylococcus bacteria are often associated with subclinical bovine mastitis. This study aimed to identify multiresistant Staphylococcus spp. associated with subclinical mastitis and the associated risk factors. Twenty-three dairy farms with a history of decrease in milk production, located in the lower Acre region, Brazil, were selected. An epidemiological questionnaire was provided in all farms. All animals were examined using the California Mastitis Test (CMT) and their milk samples were collected for bacterial culture. After isolation and identification, the disk diffusion antimicrobial susceptibility test was performed against nine classes of antimicrobials. Of the 339 cows examined using the CMT, 108 had mastitis. A total of 229 milk samples were collected from individual teats. MALDI-TOF MS found isolates belonging to eight species of Staphylococcus, in 101 of these samples. S. chromogenes (58.4%) demonstrated strongest resistance to the nine classes of antimicrobial active principles. Nineteen isolates with multidrug resistance phenotypic profile were identified. This phenotypic expression indicates wide circulation of resistant genes in this species. The presence of multidrug resistance in Staphylococcus spp. in this study was correlated with lack of water for cleaning the corral, which is a preventive factor, minimizing the transmission and persistence of pathogens in the farms.

scholarly journals Prevalence, Antimicrobial Resistance Profiles, Virulence and Enterotoxins-Determinant Genes of MRSA Isolated from Subclinical Bovine Mastitis in Egypt

Pathogens ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 362 ◽  
Author(s):  
Abdelazeem M. Algammal ◽  
Mohamed E. Enany ◽  
Reham M. El-Tarabili ◽  
Madeha O. I. Ghobashy ◽  
Yosra A. Helmy
Keyword(s):  
Antimicrobial Resistance ◽  
Susceptibility Testing ◽  
Bovine Milk ◽  
Bovine Mastitis ◽  
Subclinical Mastitis ◽  
California Mastitis Test ◽  
Total Prevalence ◽  
Health Significance ◽  
Mrsa Strains ◽  
Spa Gene

Subclinical mastitis caused by Staphylococcus aureus has worldwide public health significance. Here, we aimed to determine the prevalence of S. aureus, antimicrobial resistance profiles, and the virulence and enterotoxins determinant genes of MRSA strains that caused subclinical bovine mastitis. Milk samples were collected from 120 lactating animals (50 buffaloes and 70 dairy cattle) from different farms located in Ismailia Province (Egypt). The collected samples were investigated for subclinical mastitis using a California mastitis test. The total prevalence of S. aureus was 35.9% (84/234) with 36.3% (53/146) in cattle and 31% (31/88) in buffaloes. Antimicrobial susceptibility testing showed that 35.7% (30/84) of the isolated strains were resistant to cefoxitin, defined as methicillin-resistant S. aureus (MRSA), with 37.7% (20/53) in cattle and 32.2% (10/31) in buffaloes. Using PCR, 100% of the tested strains harbored coa and mecA genes, while 86.6% were positive for spa gene, with remarkable gene size polymorphism. Additionally, 10% of the tested strains contained the pvl gene. Further, using multiplex PCR, 26.6% of the tested samples had sea gene, two strains had sec gene and only one strain had sea and sec genes. The seb and sed genes were absent in the tested strains. In conclusion, mecA, coa and spa virulence genes were widely distributed in MRSA strains isolated from bovine milk, whereas the sea gene was the most predominant enterotoxin gene. Notably, this is the first report that emphasizes the prevalence of pvl gene of MRSA isolated from bovine milk in Egypt.

scholarly journals Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Ma. Fabiola León-Galván ◽  
José E. Barboza-Corona ◽  
A. Arianna Lechuga-Arana ◽  
Mauricio Valencia-Posadas ◽  
Daniel D. Aguayo ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Bovine Mastitis ◽  
Dairy Herd ◽  
Subclinical Mastitis ◽  
Herd Size ◽  
Clinical Mastitis ◽  
Dairy Herds ◽  
Coagulase Negative Staphylococci ◽  
California Mastitis Test ◽  
Streptococcus Uberis

Thirty-two farms (n=535cows) located in the state of Guanajuato, Mexico, were sampled. Pathogens from bovine subclinical mastitis (SCM) and clinical mastitis (CLM) were identified by 16S rDNA and the sensitivity to both antibiotics and bacteriocins ofBacillus thuringiensiswas tested. Forty-six milk samples were selected for their positive California Mastitis Test (CMT) (≥3) and any abnormality in the udder or milk. The frequency of SCM and CLM was 39.1% and 9.3%, respectively. Averages for test day milk yield (MY), lactation number (LN), herd size (HS), and number of days in milk (DM) were 20.6 kg, 2.8 lactations, 16.7 animals, and 164.1 days, respectively. MY was dependent on dairy herd (DH), LN, HS, and DMP<0.01, and correlations between udder quarters from the CMT were around 0.49P<0.01. Coagulase-negative staphylococci were mainly identified, as well asStaphylococcus aureus,Streptococcus uberis,Brevibacterium stationis,B. conglomeratum, andStaphylococcus agnetis. Bacterial isolates were resistant to penicillin, clindamycin, ampicillin, and cefotaxime. Bacteriocins synthesized byBacillus thuringiensisinhibited the growth of multiantibiotic resistance bacteria such asS. agnetis,S. equorum,Streptococcus uberis,Brevibacterium stationis, andBrachybacterium conglomeratum, but they were not active againstS. sciuri, a microorganism that showed an 84% resistance to antibiotics tested in this study.

scholarly journals Relationship of Serum Concentrations of Calcium, Beta-hydroxybutyrate and Glucose to the Occurrence of Mammary and Uterine Disorders during Early Lactation on Dairy Cattle

2020 ◽  
Vol 1 (41) ◽  
pp. 83-90
Author(s):  
Gabriel Esnaola ◽  
Waldemir antiago Neto ◽  
Félix González
Keyword(s):  
Transition Period ◽  
Serum Levels ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Cell Counting ◽  
Holstein Cows ◽  
Coagulase Negative Staphylococcus ◽  
California Mastitis Test ◽  
Using Data ◽  
Beta Hydroxybutyrate

This study aimed to relate the serum levels of energy indicators (glucose and beta-hydroxybutyrate) and calcium to the occurrence of uterine and mammary diseases in the transition period of Holstein cows. One hundred and fifteen Holstein cows from a commercial herd were monitored starting one week before calving and during the first 2 weeks after calving. The presence of subclinical mastitis was monitored for 3 weeks postpartum using the California Mastitis Test (CMT) and somatic cell counting. Positive samples of subclinical mastitis were analyzed for bacterial identification. Clinical mastitis was monitored for 6 weeks postpartum, using data that were recorded by the farmer based on some established definitions. The presence of uterine disorders was identified for 3 weeks postpartum by clinical examination. Blood collections were made from all cows for determining calcium, glucose, and β-hydroxy-butyrate (BHB) the week before calving and twice during the postpartum (days 5 and 15 postpartum). A group of 70 healthy cows was used as control. Clinical mastitis was identified in 20% of the cows, subclinical mastitis in 10.4% and clinical metritis in 8.7% of the cows. The bacterial agents with the highest occurrence in subclinical mastitis cases were coagulase-negative Staphylococcus and Streptococcus. No relationships were observed between any of the biochemical metabolite studied and the uterine and mammary diseases in the cows during the studied period

scholarly journals Kejadian mastitis subklinis pada kambing perah Peranakan Etawa di desa Bangelan kecamatan Wonosari kabupaten Malang

2020 ◽  
Vol 9 (3) ◽  
pp. 60
Author(s):  
Nur Zaini Nanda Mardian ◽  
Soeharsono Soeharsono ◽  
Nenny Harijani ◽  
Budiarto Budiarto ◽  
Herry Agoes Hermadi ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Milk Production ◽  
Clinical Symptoms ◽  
Subclinical Mastitis ◽  
Dairy Goat ◽  
California Mastitis Test

Etawa crossbred goat is one species of goat that is raised for milk production. One of the diseases that often occur in dairy livestock is mastitis, in which there is inflammation of the mammary gland. It is common in goat, that mastitis does not show clinical symptoms (subclinical). This research aims to determine the incidence of subclinical mastitis in Etawa crossbred goats in Bangelan village Wonosari district Malang regency. This research involved 25 respondent farms, which are Etawa crossbred dairy goat farms. In this research, subclinical mastitis cases were directly detected using California Mastitis Test (CMT). The results showed that out of 70 samples collected, 41 (58.6%) samples were positive. These CMT positive goats were from 88% of the respondent farms.

scholarly journals Effect of the type of milking on mammary gland examination in Saanen goats

2020 ◽  
Vol 87 ◽  
Author(s):  
Jean Silva Ramos ◽  
Viviani Gomes ◽  
Mariana Porto Matazo ◽  
Cynthia Pereira da Costa e Silva ◽  
Vinicius Alvim Passos Baldacim ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Physical Examination ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Physical Exam ◽  
Inappropriate Use ◽  
Machine Milking ◽  
California Mastitis Test ◽  
Traumatic Lesions

ABSTRACT This research evaluated the effect of milking on the physical exam of the mammary gland (MG) and milk examination in Saanen goats. Six properties were selected, four farms were used after evaluation of criteria selection and distributed in two groups according to the milking method: manual or mechanical. The physical examination of MG was performed using the techniques of inspection and palpation, in addition to the examination of the milk by strip cup, California Mastitis Test, somatic cell count, and bacteriological exam. It was possible to detect an association between the milking method with the most of physical exam of the MG: inspection of the MG (p = 0.001), inspection of the teat (p = 0.002), palpation of the MG (p = 0.054) and palpation of the teat (p = 0.036). The machine milking had an association with the reduction of the volume of MG (OR = 6.00), reduction of the teat size (OR = 16.19), and firm consistency of mammary parenchyma (OR = 2.39). The use of machine milking represented a less risk for an increase of the volume of the MG volume (OR = 0.288) and the presence of cisternitis (OR = 0.490). The association between the SCC and the type of milking was also detected (p = 0.002). Machine milking was associated with changesin the physical exam of MG and milk examination compatible with traumatic lesions. This information indicates inappropriate use of milking equipment in the dairy goat’s properties.

scholarly journals Mammary gland health of Santa Inês ewes at the drying and puerperium and evaluation of a dry-off terapy with gentamicin

2018 ◽  
Vol 38 (12) ◽  
pp. 2194-2200
Author(s):  
Priscilla F.V. Pereira ◽  
Ana P. Reway ◽  
Aline Félix ◽  
Edsel A. Beutemmüller ◽  
Lucienne G. Pretto-Giordano ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Somatic Cell Count ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Control Group ◽  
Coagulase Negative Staphylococcus ◽  
California Mastitis Test ◽  
Important Health ◽  
Santa Inês ◽  
Staphylococcus Spp

ABSTRACT: Mastitis represents an important health problem for Santa Inês breed, causing losses to the producer, due to loss of ewes or the decrease in weight gain of lambs. The aim of this work was to assess the health of the mammary gland of Santa Inês ewes at the drying and puerperium and to investigate the efficacy of a dry-off therapy with gentamicin. In this study, 64 ewes were divided in a control group (GC) and treatment group (GT), and the health of the mammary gland was assessed at the drying and puerperium. The GT ewes received 250mg of gentamicin (Gentocin® DryCow/Schering-Plough, product indicated for use in dairy cows) in each mammary half. For diagnosis, clinical examination, California Mastitis Test, somatic cell count and milk culture was performed. In the GC, of the 45 (70.3%) healthy mammary halves at the drying, 12 developed subclinical mastitis and nine clinical mastitis at the puerperium. In the GT, among 51 (79.7%) healthy mammary halves at the drying, six developed subclinical mastitis and 11 clinical mastitis at the puerperium. No association was observed between treatment and the occurrence of mastitis at puerperium. Of the 19 (29.7%) mammary halves of the GC that presented subclinical mastitis at the drying, three remained with subclinical mastitis and five developed clinical mastitis at the puerperium. In the GT, of the 13 (20.3%) mammary halves that had subclinical mastitis at the drying, four remained with subclinical mastitis and four developed clinical mastitis. No association was observed between treatment and cure or persistence of mastitis at the puerperium. The main microorganisms isolated, at the drying and puerperium, from animals with subclinical or clinical mastitis were Staphylococcus spp., predominantly coagulase negative Staphylococcus (CSN). At the puerperium, 29 cases of clinical mastitis occurred, 19 with isolation, where 10 were CNS and six S. aureus. Mannheimia haemolytica was isolated in one case of subclinical mastitis and other of clinical mastitis. News protocols and different ways of handling at drying and at puerperium must be investigated.

scholarly journals Prevalence of Bovine Mastitis in the Western Region of Cameroon

2021 ◽  
pp. 1-5
Author(s):  
Müller FOTSAC DZOUSSE ◽  
◽  
Marc K. KOUAM ◽  
Keyword(s):  
Bovine Mastitis ◽  
Clinical Signs ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Feeding Method ◽  
Lactating Cows ◽  
Stage Of Lactation ◽  
California Mastitis Test ◽  
Farm Type ◽  
Age Range

Mastitis is the major disease in milking cows causing huge losses to the dairy industry worldwide. It is in view to contribute to the eradication of this pathology that the present study was carried out in cattle farms of the West Region of Cameroon from March 2020 to November 2020. A total of 473 lactating cows of local and improved breeds were screened for mastitis. Screening for clinical and sub-clinical mastitis was based on the observation of clinical signs and on the use of the California Mastitis Test (CMT) respectively. The overall prevalence was 34.88%, among which 9.72% and 25.16% represented clinical and subclinical mastitis respectively. In summary, the factors influencing the occurrence of mastitis were: the divisions surveyed (p<0.001), breed type (p=0.003), age range (p<0,001), farm type (p=0.05), soil type (p<0,001), cleaning frequency (p<0.001), feeding method (p<0.001), hygiene of udder (p=0.05) and stage of lactation (p=0.02). The results obtained allow us to recommend an improvement of the breeding conditions to prevent mastitis, because once mastitis appears in a farm, it becomes very difficult to eradicate it.

scholarly journals Sanitary practices associated with animal welfare in the control of mastitis in the dairy herd

2021 ◽  
Vol 10 (17) ◽  
pp. e102101724467
Author(s):  
Mirian Yumi Makatu ◽  
Jefferson Filgueira Alcindo ◽  
Gabriela Cortellini Ferreira Ramos ◽  
Katia Denise Saraiva Bresciani ◽  
Márcia Marinho
Keyword(s):  
Animal Welfare ◽  
Health Management ◽  
Bovine Mastitis ◽  
Agricultural Practices ◽  
Dairy Herd ◽  
Subclinical Mastitis ◽  
Educational Materials ◽  
Lack Of Information ◽  
Prophylactic Measures ◽  
Improved Performance

Agricultural practices are a powerful tool with health management contributing to the improved performance of production of animal welfare. The study aimed to verify that farming practices are important tools in the control of bovine mastitis and the degree of knowledge of the producers with respect to the practices. Guided visits were carried out the rural properties, when they were distributed educational materials and tools, made from recyclable material, used in the management of sanitary practices related to animal welfare. To evaluate the physiological profile were collected biological samples such as: blood, feces and milk, and for assessing the sanity and animal welfare were used a questionnaire. Blind 20 cows were selected for a total of 79 rooms of which breast; 36 rooms were negative to the CMT, but 12 were of these were positive to Staphylococcus and microbiology microorganism of higher occurrence. Animal welfare was compromised by the incidence of Subclinical Mastitis, infestation of flies and the absence of prophylactic measures. It is concluded that the use of good agricultural practices is a suitable tool associated with the animal welfare and important in the identification of bovine mastitis, and a lack of information and knowledge regarding best practices, especially in relation to preventive management.

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