scholarly journals Lithium modulates the chronic stress-induced effect on blood glucose level of male rats

2010 ◽  
Vol 62 (2) ◽  
pp. 289-295 ◽  
Author(s):  
Natasa Popovic ◽  
Snezana Pajovic
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Chronic Stress ◽  
Glucose Level ◽  
Restraint Stress ◽  
Elevated Serum ◽  
Corticosterone Level ◽  
Male Rats ◽  
Chronic Restraint Stress ◽  
Serum Corticosterone

In the present study we examined gross changes in the mass of whole adrenal glands and that of the adrenal cortex, as well as the serum corticosterone and glucose level of mature male Wistar rats subjected to three different treatments: animals subjected to chronic restraint-stress, animals injected with lithium (Li) and chronically stressed rats treated with Li. Under all three conditions we observed hypertrophy of whole adrenals, as well as the adrenal cortices. Chronic restraint stress, solely or in combination with Li treatment, significantly elevated the corticosterone level, but did not change the blood glucose level. Animals treated only with Li exhibited an elevated serum corticosterone level and blood glucose level. The aim of our study was to investigate the modulation of the chronic stress-induced effect on the blood glucose level by lithium, as a possible mechanism of avoiding the damage caused by chronic stress. Our results showed that lithium is an agent of choice which may help to reduce stress-elevated corticosterone and replenish exhausted glucose storages in an organism.

scholarly journals Neurotoxic Effect of Fipronil in Male Wistar Rats: Ameliorative Effect of L-Arginine and L-Carnitine

Biology ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 682
Author(s):  
Yasmina K. Mahmoud ◽  
Ahmed A. Ali ◽  
Heba M. A. Abdelrazek ◽  
Tahany Saleh Aldayel ◽  
Mohamed M. Abdel-Daim ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Histopathological Examination ◽  
Memory Performance ◽  
Elevated Serum ◽  
Corticosterone Level ◽  
Male Rats ◽  
Spatial Learning And Memory ◽  
Serum Corticosterone ◽  
Group I ◽  
Ameliorative Effect

The ameliorative effect of L-arginine (LA) and L-carnitine (LC) against fipronil (FPN)-induced neurotoxicity was explored. In this case, 36 adult male rats were randomly divided into six groups: group I received distilled water, group II received 500 mg/kg LA, group III received 100 mg/kg LC, group IV received 4.85 mg/kg FPN, group V received 4.85 mg/kg FPN and 500 mg/kg LA and group VI received 4.85 mg/kg FPN and 100 mg/kg LC for 6 weeks. Cognitive performance was assessed using Barnes maze (BM). Serum corticosterone, brain total antioxidant capacity (TAC), malondialdehyde (MDA) and dopamine were measured. Histopathology and immunohistochemistry of ionized calcium-binding adaptor (Iba-1), doublecortin (DCX) and serotonin (S-2A) receptors were performed. Fipronil induced noticeable deterioration in spatial learning and memory performance. In addition, FPN significantly (p < 0.05) diminished brain antioxidant defense system and dopamine coincide with elevated serum corticosterone level. Histopathological examination revealed degenerative and necrotic changes. Furthermore, Iba-1 and DCX were significantly expressed in cortex and hippocampus whereas S-2A receptors were significantly lowered in FPN group. However, administration of LA or LC alleviated FPN-induced deteriorations. In conclusion, LA and LC could be prospective candidates for mitigation of FPN-induced neurotoxicity via their antioxidant, anti-inflammatory and neuropotentiating effects.

scholarly journals Pengaruh suplementasi β-carotene terhadap kadar glukosa darah dan kadar malondialdehida pada tikus sprague dawley yang diinduksi Streptozotocin

2014 ◽  
Vol 2 (2) ◽  
pp. 41-46
Author(s):  
Elida Soviana ◽  
Banundari Rachmawati ◽  
Nyoman Suci Widyastiti
Keyword(s):  
Free Radicals ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Negative Control ◽  
Male Rats ◽  
Sprague Dawley ◽  
Significant Difference ◽  
Decrease Blood Glucose ◽  
Β Carotene

Background : Hyperglycemia on diabetes mellitus can cause increasing of free radicals production. Free radicals caused lipid peroxidation reaction by forming malondialdehyde (MDA). β-carotene has antioxidant activity may inhibit the formation of ROS.Objective : To prove the effect of multilevel doses β-carotene 1 mg/kg BW, 20 mg/kg BW and 20 mg/kg BW on alternate days within 30 days orally supplementation on blood glucose level and MDA level on Sprague Dawley male rats induced by streptozotocin (STZ). Methods : Thirty rats were randomly divided into 5 groups: X1=Negative control/diabetic, X2 (STZ 40 mg/kg BW + BC 1 mg/kg BW), X3 (STZ 40 mg/kg BW + BC 10mg/kg BW), X4 (STZ 40 mg/kg BW + BC 20 mg/kg BW), X5 (technic control/non diabetic). β-Carotene supplementation was given by nasogastric tube on alternate days within thirty days. Blood glucose level was measured by GOD-PAP and MDA level by ELISA with TBARS methods. Data was analized using paired t-test, wilcoxon, one way anova and post hoc bonferroni. Results : there was a significant difference of blood glucose level (p = 0,0001) and MDA level (p = 0,0001) after suplementation β-carotene on alternate days within 30 days orally. β-carotene 10 mg/kg BW was the most effective and efficient dose to lowering blood glucose, while 20 mg/kg BW to lowering MDA level. Conclusion : The multilevel doses β-carotene (1 mg/kg BW, 10 mg/kg BW and 20 mg/kg BW) on alternate days within 30 days orally supplementation can decrease blood glucose and MDA level. β-carotene 10 mg/kg BW is the most effecetive and efficient to decrease blood glucose and β-carotene 20 mg/kg BW to decrease MDA level.

scholarly journals Manalagi Apple Vinegar (Malus sylvestris Mill) as Anti Diabetic to Alloxan Induced Wistar White Male Rat

2019 ◽  
Vol 2 (1) ◽  
pp. 44
Author(s):  
Valentina Verrel Purnomo ◽  
Agustinus Sareh Arjono Tjandra ◽  
Risma Risma
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
White Male ◽  
Test Method ◽  
Male Rats ◽  
Male Rat ◽  
Control Group ◽  
Standard Diet ◽  
Malus Sylvestris

<p>Apple vinegar is estimated to be able to cure diabetes. Vinegar is able to control the increase of glicemic index. Apple has antioxidant substance such as quercetin and chlorogenic acid. Manalagi apple could be found easily in Indonesia.</p><p>            This research aims to reveal the effect of Manalagi apple vinegar (<em>Malus sylvestris </em>Mill) towards blood glucose level of alloxan induced Wistar white male rats (<em>Rattus norvegicus</em>).</p><p>            This research is experimental laboratory using <em>Experimental </em>methods. The samples are 30 white rats divided into three groups for 31 days, 1) group which only given standard diet; 2) group that induced by 120mg/dL alloxan; 3) group that induced by 120mg/dL alloxan and Manalagi apple vinegar 0.27ml/100gram weight for 14 days. The measurement of blood serum glucose level is done on day twenty four using homogenous colorimetric enzymatic test method for all groups.</p><p>            Blood glucose average in group with only given standard diet as negative control group (182.33 mg/dL), group which induced by 120mg/dL alloxan as positive control group (205.2 mg/dL), group which induced by 120mg/dL alloxan and Manalagi apple vinegar as treatment group (212.25 mg/dL). The result of <em>One-Way Anova </em>significance<em> </em>is 0.229.</p><p>            The conclusion of this research is that Manalagi apple vinegar treatment doesn’t able to decrease the blood glucose level of white Wistar male rats significantly.</p><p> </p><strong>Keywords </strong>: <em>Malus sylvestris </em>Mill, Blood glucose, Alloxan.

scholarly journals Vitamin D Regulatory Effect on Restraint Stress-Induced Changes on Serum Corticosterone and Hippocampus BDNF Levels in Male Rats

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Katayoun Sedaghat ◽  
Sara Choobdar ◽  
Ahmad Reza Bandegi ◽  
Zahra Ghods
Keyword(s):  
Vitamin D ◽  
Chronic Stress ◽  
Restraint Stress ◽  
Male Rats ◽  
Negative Effects ◽  
Bdnf Level ◽  
Serum Corticosterone ◽  
Protein Levels ◽  
Induced Changes ◽  
The Impact

Background: Chronic stress exerts negative effects on cognitive functions through inducing changes in the hippocampus. Brain-derived neurotrophic factor (BDNF) is an essential factor in cognitive activities, which is considerably reduced under chronic stress. 1,25(OH)2 vitamin D plays neuroprotective roles partially by regulating the expression of various neurotrophic factors. Objectives: Since few studies have studied the impact of vitamin D on BDNF level, we conducted this brief experiment to understand the role of vitamin D in maintaining hippocampal BDNF protein levels by using restraint as a model of chronic stress in rats. Methods: Rats underwent restraint stress 3 h/day for 28 days, during which they received vitamin D (5, 10 μg/kg) or its vehicle (IP, twice weekly). After the stress period, serum corticosterone (CORT) and hippocampus BDNF protein levels were measured. Results: Restraint stress increased serum CORT (P < 0.001) and reduced BDNF protein levels (P < 0.001) as compared to the non-stress group. Vitamin D markedly maintained BDNF level close to normal (P < 0.001), but did not change CORT level significantly. Conclusions: This study demonstrated that 3h/day of chronic restraint stress for 28 days boosted serum CORT and declined hippocampal BDNF levels, similar to stronger restraint stress models. Vitamin D maintained BDNF level close to normal in the hippocampus, but it did not affect CORT level significantly.

Antidiabetic Activity of the Methanolic Extracts of Thuja occidentalis Twings in Alloxan-induced Rats

2019 ◽  
Vol 5 (2) ◽  
pp. 126-131
Author(s):  
C.K. Tyagi ◽  
Pawan Porwal ◽  
Neeraj Mishra ◽  
Amit Sharma ◽  
A. Chandekar ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Elevated Serum ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Thuja Occidentalis ◽  
Methanolic Extracts ◽  
Elevated Serum Cholesterol

Objective and Background: In hyperglycemic and alloxan-induced diabetic rats, the effect of Thuja occidentalis methanolic residue extract on blood glucose levels and some biochemical parameters were investigated. Methods: Significantly decreased blood glucose level by 22.85% and 27.66%, in hyperglycemic rats, respectively after 3 h, were seen in a single oral administration of the extract at a dose of 200 and 400 mg/kg. Blood glucose level was decreased by 50% in alloxan-induced diabetic rats within three weeks of daily treatment of Thuja occidentalis methanolic residue extract (200 and 400 mg/kg p.o). Alloxan-induced diabetic rats showed significant hypercholesterolemia in comparison with the control in alloxan induced rats. Results: In both normal and diabetic rats, there was also a significant decrease of elevated serum cholesterol and triglycerides. Hypertriglyceridemia was also shown to be prevented by treatment with plant extract (200 and 400mg/kg p.o) (p<0. 05). As compared with the control animals, diabetic control rats did not show any change in a level of creatinine and urea. Conclusion: Thuja occidentalis treatment may improve glucose homeostasis in alloxaninduced diabetes and alleviate kidney and liver function. Thuja occidentalis twigs could be a potential source of the new oral antidiabetic drug.

scholarly journals EFFECT OF CADMIUM EXPOSURE ON INCREASING RISK OF DIABETES MELITUS THROUGH THE MEASUREMENT OF BLOOD GLUCOSE LEVEL AND LIVER GLUCOKINASE ACTIVITY IN RATS

2017 ◽  
Vol 13 (2) ◽  
pp. 137 ◽  
Author(s):  
Iskandar Thalib ◽  
Windy Yuliana Budianto ◽  
Eko Suhartono
Keyword(s):  
Drinking Water ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Normal Activity ◽  
Male Rats ◽  
Research Subjects ◽  
Increasing Risk ◽  
Cd Exposure ◽  
Diabetes Melitus

Abstract: This present study was undertaken to investigate the effect of cadmium (Cd) exposure on an increasing risk of Diabetes Melitus (DM), through the measurement of blood glucose level and liver glucokinase activity in rats. The subjects that used in this study are 15 male rats (Rattus novergicus) with normal activity, 2-3 months old, and weighing 300±10 gram. The research subjects the divided into 3 groups; P0 group are given commercial fed rats diets only; P1 are given commercial fed rats + Cd with a concentration of 3 mg/l in drinking water for 1 day (acute); and P2 are given commercial fed rats+Cd with a concentration of 3 mg/l in drinking water for 4 weeks (subacute). The results of this present study shows that treatment with Cd significantly increase the levels of blood glucose (P < 0,05). The result also showed that treatment with Cd can increase the Km value of liver glucokinase, and it means Cd exposure can decrease the affinity between glucose and glucokinase. The present study demonstrated that Cd exposure could increase the risk of DM through increased the blood glucose and decrease the activity of liver glucokinase. Keywords: Cadmium, glucose metabolism, glucose, glucokinase

scholarly journals Ginkgo biloba modulates hippocampal BDNF expression in a rat model of chronic restraint stress-induced depression

2020 ◽  
Vol 24 (4) ◽  
pp. 285-297
Author(s):  
Seyed Abdolmajid Ayatollahi ◽  
◽  
Shahrokh Khoshsirat ◽  
Ali Asghar Peyvandi ◽  
Omidvar Rezaei ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Protein Expression ◽  
Ginkgo Biloba ◽  
Restraint Stress ◽  
Corticosterone Level ◽  
The Body ◽  
Male Rats ◽  
Bdnf Expression ◽  
Serum Corticosterone ◽  
Repeated Restraint Stress

Introduction: Mood disorders such as depression and anxiety disorders have been affecting a relatively high proportion of the world's population. Neuroplasticity hypothesis of depression proposes that lack of brain-derived neurotrophic factor (BDNF) can cause structural changes in the brain. The extract of Ginkgo biloba (Gb) leaves can restore much of the damage in the nervous system. We examined the antidepressant role of Gb extract (EGb 761) on BDNF expression modulation in the hippocampus of rats subjected to repeated restraint stress (RRS). Methods: Adult male rats were randomly divided into 10 groups: control, control-vehicle treated, stress, stress-vehicle treated, as well as three control and three experimental groups pretreated with EGb (15, 30, 60mg/kg, IP daily) for 21 days. They underwent restraint stress on a daily basis, 6 hours for 21 consecutive days. Weight changes, locomotor activity and forced swim test (FST) were employed to assess depressive-like symptoms. The serum corticosterone level was also measured by ELISA. Hippocampal BDNF DNA methylation and protein expression were assayed by methylation sensitive restriction enzymes (Real Time PCR) and Western-blotting respectively in all groups. Results: Pre-treatment with 30 and 60 mg/kg/day of Gb extract significantly attenuated depressive-like effects in the body weight, FST and serum corticosterone level in RSS rats compared to control groups. Further, it inhibited chronic stress-induced alterations in the hippocampal BDNF DNA methylation and protein expression. Conclusion: These findings suggest that Gb can induce an antidepressant role through its modulation effect on the hippocampal BDNF expression.

Sign in / Sign up

Export Citation Format

Share Document