scholarly journals Immunoglobulins G, A, M and circulatory immune complexes in patients with laryngeal carcinoma

2009 ◽  
Vol 56 (3) ◽  
pp. 45-49
Author(s):  
M.V. Dimitrijevic ◽  
V.Z. Djordjevic ◽  
N.A. Arsovic ◽  
P.D. Jeremic ◽  
N.A. Slijepcevic ◽  
...  
Keyword(s):  
Blood Serum ◽  
Immune Complexes ◽  
Laryngeal Carcinoma ◽  
Clinical Course ◽  
Malignant Tumors ◽  
Strong Association ◽  
Circulating Immune Complexes ◽  
Surgical Removal ◽  
Immunological Parameters ◽  
Humoral Immune

Introduction Malignant tumors of the head and neck represent 5% of all malignancies, of which the most common are tumors of the larynx and oral cavity. In the blood serum of patients with malignant tumors increased levels of immunoglobulins and circulating immune complexes have been detected, with a diverse relevance to the clinical course and prognosis of the disease. Aim Due to contradictory findings of the correlation between the humoral immune response and the clinical course of the disease, we examined 42 male patients with laryngeal carcinoma. All patients underwent surgery, of which 15 patients were treated postoperatively with radiotherapy. The changes in the levels of immunoglobulins and circulating immune complexes in the blood serum were recorded and evaluated. Results Analysis of the results showed an immunological disorder of an abnormal level of circulating immune complexes in the blood serum that normalized after the surgical removal of the tumor. The levels of immunoglobulins G and A were abnormal during the whole postoperative period of examination. Conclusion The normalizing of the levels of circulating immune complexes in the blood serum, after surgical removal of the tumor, shows a strong association between the two, and this could consequently mean that it could be used as a prognostic tool, particularly in correlation with other immunological parameters.

scholarly journals Immune related prognosis biomarkers for outcomes of joint preservation surgery in children with ischemic deformity of the proximal femur (preliminary results)

2020 ◽  
Vol 26 (4) ◽  
pp. 516-520
Author(s):  
M.P. Teplenky ◽  
◽  
M.V. Chepeleva ◽  
E.I. Kuznetsova ◽  
◽  
...  
Keyword(s):  
Proximal Femur ◽  
Immune Complexes ◽  
Immune Status ◽  
Circulating Immune Complexes ◽  
Immunological Parameters ◽  
Humoral Immune ◽  
Joint Preservation ◽  
Group I ◽  
Low Levels ◽  
Group Ii

Objective Assess the immune status of children with ischemic deformity of the proximal femur taking into account the treatment outcome. Material and methods Preoperative assessment of the immune status was performed for 16 adolescents with ischemic deformity of the proximal femur. Patients' age was 14.4 ± 0.45 years. Immunological parameters of 12 adolescents with satisfactory (group I) and 4 patients with poor (group II) anatomical and functional outcome of surgical treatment were compared. Peripheral blood lymphocyte typing was produced with laser cytometry assay, and IgA, IgM, IgG, IgE, IL-18, IFNγ and circulating immune complexes were quantified with enzyme-linked immunoassay. Results Сomparative analysis of preoperative immunological parameters showed statistically significant decline of Т-helper cells (СD3+СD4+), high levels of activated СD3+CD25 and СD3+HLADR T lymphocytes, low levels of activated NК cells (СD8dimCD38+) and IFNγ in group II. Humoral immune parameters indicated to low levels of IgA, IgM, IgG and higher levels of circulating immune complexes in group II relative to group I. Conclusion Preliminary data obtained suggest that cellular and humoral immune deficiency can be associated with unfavorable outcome of joint preservation procedure. Such immunological parameters as T lymphocytes subpopulation, immunoglobulins, circulating immune complexes and IFNγ can be used as significant prognostic factors for preoperative planning and postoperative rehabilitation of adolescents with ischemic deformity of the proximal femur.

scholarly journals Influence of cryptosporidiosis agent on biochemical and immunological parameters of blood of calves

2018 ◽  
Vol 20 (88) ◽  
pp. 136-140 ◽  
Author(s):  
V. V. Zhurenko ◽  
O. V. Zhurenko
Keyword(s):  
Immune System ◽  
Blood Serum ◽  
Immune Complexes ◽  
Structural Changes ◽  
Specific Interaction ◽  
The Body ◽  
Circulating Immune Complexes ◽  
Total Protein Content ◽  
Immunological Parameters ◽  
Antigen Antibody

Cows, young cattle and calves of black-and-bark breeds, spontaneously affected by the causative agent Cryptosporidium parvum, were selected for the research. Blood for research was taken from calves in the morning before feeding from the jugular vein. Biochemical parameters of blood serum were determined using a biochemical analyzer of the closed type. Changes in the activity of serum enzymes in the initial stages of the disease are not specific, they indicate a compensatory reaction of liver cells and biliary tract. Effect of Cryptosporidium in calves body accompanied by severe disorders that affect the metabolism of proteins, carbohydrates and enzymes. These changes lead to structural changes in the organs at the cellular level. Serum infested animals significantly reduced total protein content of 25.5% (P < 0.001), albumin – by 14.6% (P < 0.01), the concentration of glucose in – 21.2% (P < 0.01), carotene content – 12.6% (P < 0.05), the level of calcium and phosphorus – 17.5 and 18% (P < 0.05), respectively, and increased total bilirubin content by 23% (P < 0.001), indicating significant violations of the protein synthesis and detoxification processes in the liver and the tension in the metabolism of their body. Reduced glucose concentration in blood serum is due to the fact that in the body of sick animals there have been increased costs for maintaining the energy needs of its own organism. In conducting studies for 35 days in animals in the experimental group noted an increase in the concentration of circulating immune complexes by 10.5% relative to control. Increasing the concentration of circulating immune complexes in serum of calves, patients with cryptosporidiosis, indicates the presence of specific interaction antigen-antibody and decrease the activity of the humoral link of the immune system. In the determination of immunosuppressive proteins – serum cords in serum of calves, it was found that at 7, 14 days their concentration was within the physiological limits. Increasing the concentration of circulating immune complexes in serum of calves, patients with cryptosporidiosis, indicates the presence of specific interaction antigen-antibody and decrease the activity of the humoral link of the immune system.

scholarly journals Role of Circulating Immune Complexes in the Pathogenesis of Canine Leishmaniasis: New Players in Vaccine Development

2021 ◽  
Vol 9 (4) ◽  
pp. 712
Author(s):  
Cristina Cacheiro-Llaguno ◽  
Nuria Parody ◽  
Marta R. Escutia ◽  
Jerónimo Carnés
Keyword(s):  
Immune Complexes ◽  
Vaccine Development ◽  
Correct Diagnosis ◽  
Circulating Immune Complexes ◽  
Response To Treatment ◽  
Canine Leishmaniasis ◽  
Leishmania Infection ◽  
Serum Samples ◽  
Humoral Immune

During canine visceral leishmaniasis (CanL), due to Leishmania infantum (L. infantum), uncontrolled infection leads to a strong humoral immune response. As a consequence of the production of high antibody levels and the prolonged presence of parasite antigens, circulating immune complexes (CIC) are formed, which can be deposited in certain organs and tissues, inducing vasculitis, uveitis, dermatitis and especially glomerulonephritis and renal failure. A method to detect CIC and quantify their levels in serum samples from dogs infected with L. infantum has been recently described. It allowed demonstration of a correlation between CIC levels and disease severity. Thus, CIC measurement may be useful for diagnosis, assessment of disease progression and monitoring response to treatment. This is an interesting finding, considering that there remains an urgent need for identification of novel biomarkers to achieve a correct diagnosis and for optimal disease staging of dogs suffering from Leishmania infection. The objective of the present review is to shed light on the role of CIC in CanL, as well as to highlight their potential use not only as diagnostic and prognostic biomarkers but also as a valuable tool in vaccine development and new immunotherapy strategies to prevent or control disease outcome.

scholarly journals Influence of Marmix premix on the state of lipid peroxidation and indices of non-specific resistance of the organism of pregnant mares with microelementosis

2019 ◽  
Vol 10 (1) ◽  
pp. 87-91
Author(s):  
A. R. Shcherbatyy ◽  
L. G. Slivinska ◽  
B. V. Gutyj ◽  
V. L. Fedorovych ◽  
B. O. Lukashchuk
Keyword(s):  
Lipid Peroxidation ◽  
Blood Serum ◽  
Immune Complexes ◽  
Positive Influence ◽  
Lysozyme Activity ◽  
The State ◽  
Circulating Immune Complexes ◽  
Lipid Hydroperoxides ◽  
Diene Conjugates ◽  
Lipid Peroxidation Products

We observed the processes of lipid peroxidation, characterized by increased content of intermediate and its end products (diene conjugates, lipid hydroperoxides, malonic dialdehyde), in the blood of the pregnant mares. The changes of the data of indicators in the organism of the mares at the 9–11 months of pregnancy were established. The level of diene conjugates in the blood of animals was 3.6 ± 0.12 μmol/l, 33.3 and 44.0%, which was higher compared to non-pregnant mares and mares at 4th month of pregnancy. The concentration of lipid hydroperoxides in the blood of mares increased by 100% and by 42.9% in the blood of non-pregnant mares and mares at 4th month of pregnancy. The concentration of malondialdehyde in the blood of mares increased by 75.0%, 51.2% and 25.0% compared to non-pregnant mares and mares at 4th and 7th month of pregnancy, respectively. The results of our research showed that the mineral-vitamin premix Marmix had a positive influence on the state of lipid peroxidation products, contributed to their lowering in the blood of pregnant mares, which demonstrated antioxidant action and stimulated the function of the immune system. It was established that the bactericidal and lysozyme activity of the blood serum and the concentration of circulating immune complexes increases in the mares with lack of trace elements. It was shown that feeding mares during the 9–11 months of pregnancy with the mineral-vitamin premix Marmix had a positive influence on the state of humoral factors of nonspecific resistance. In particular, the concentration of bactericidal activity of serum increased by 31.0%, lysozyme activity of blood serum – 45.4% and contributed to the decrease of the concentration of circulating immune complexes in serum by 3.8 times. The use of mineral-vitamin premix Marmix during 60 days in feeding pregnant mares caused a restoration of the clinical status, a decrease in the content of lipid peroxidation products.

scholarly journals Evolution of humoral immunity effectors in four flocks of sheep with symptoms of contagious ecthyma, from Santău village, Romania

2021 ◽  
Vol 26 (2) ◽  
pp. 2361-2369
Author(s):  
ELISABETA-RAFILA MESTESANU ◽  
Keyword(s):  
Immune Complexes ◽  
Clinical Signs ◽  
Individual Characteristics ◽  
Circulating Immune Complexes ◽  
Contagious Ecthyma ◽  
Immunological Parameters ◽  
Secondary Infections ◽  
Welfare Implications ◽  
The Individual ◽  
Two Parameters

Contagious ecthyma or contagious pustular dermatitis is a debilitating condition located on the skin of sheep and goats, with a zoonotic character. Contagious ecthyma is a disease with economic and animal welfare implications, rarely being fatal if the host organism suffers from immunosuppression or if secondary infections occur. Similar to other members of the Poxviridae family, immunity to contagious ecthyma is mediated by both innate and adaptive immune responses. Following the invasion of the contagious ecthyma virus, immunoglobulins are mobilized that specifically bind to the contagious ecthyma virus to form immune complexes, which are then eliminated by defense system to protect tissues from damage. The level of circulating immune complexes and immunoglobulins can be quantified and can be an indicator of the stage of infection. In the present study we aimed to investigate whether there is a link between the individual characteristics (sex, age, severity of clinical signs) of sheep with orf symptoms and the evolution of circulating immune complexes and total immunoglobulins. In addition to these investigations, we aimed to follow the evolution of these immunological parameters compared to the evolution in clinically healthy animals. Investigations of the serum revealed that levels of these two parameters (total Ig and CIC), can be influenced by the individual characteristics of sheep affected by the contagious ectima and shows differences compared to clinically healthy animals.

DYNAMICS OF THE LEVEL OF CIR-CULATING IMMUNE COMPLEXES IN BLOOD SERUM IN RABBITS UNDER MODEL STRESSES

2020 ◽  
Vol 3 ◽  
pp. 135-138
Author(s):  
O. V. Kryachko ◽  
◽  
L.A. Lukoyanova ◽  
Keyword(s):  
Blood Serum ◽  
Immune Complexes ◽  
Live Weight ◽  
Circulating Immune Complexes ◽  
Stress Factors ◽  
Innate Immune Responses ◽  
Stress Effects ◽  
Transport Stress ◽  
Post Stress ◽  
Dorsal Position

A small amount of circulating immune complexes is determined in a healthy body, and it naturally increases when there is an antigen in the circulation. Stressful situations can inhibit the implementation of immune protection, both adaptive and innate immune responses. The purpose of this study was to study the dynamics of the level of circulating immune complexes in blood serum after modeling single and combined stress effects in rabbits. 10 male rabbits were used for the experiment. The animals were 8 months old and had a live weight of 3.40±0.73 kg. Transport stress was caused by transporting animals in a car. Immobilization stress was modeled by fixing rabbits in a fixation de-vice for small animals by their legs in the dorsal position. In the final series of experi-ments, a combination of actions was per-formed (immobilization, then, after three days, transportation). As a result of studies it was found that the reaction of animals to stress was characterized by decreased level of the CIC, during transportation (by 1.2 times (p<0.05) and immobilization (1.6 times, p<0.05), the combination of stress factors showed a similar effect. In the post-stress period, we observed in all cases an increase in the CIC level in the blood of rab-bits, which exceeded the CIC values both at the time of the stress reaction and the level of intact animals.

scholarly journals Molecular Aggregation in Immune System Activation Studied by Dynamic Light Scattering

Biology ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 123
Author(s):  
Elena Velichko ◽  
Sergey Makarov ◽  
Elina Nepomnyashchaya ◽  
Ge Dong
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Blood Serum ◽  
Immune Complexes ◽  
Circulating Immune Complexes ◽  
Scattering Method ◽  
Dynamic Light Scattering Method ◽  
Immune System Activation ◽  
Healthy Donors ◽  
Biomedical Diagnostics

Determination of the concentration and size of the circulating immune complexes in the blood is an essential part of diagnostics of immune diseases. In this work, we suggest using the dynamic light scattering method to determine the sizes of circulating immune complexes in blood serum. By the dynamic light scattering spectrometer, we found that for healthy and sick donors, the size and concentration of circulating immune complexes differed significantly. The dynamics of formation of these complexes were also examined in this work. It was shown that the formation of immune complexes in the blood of healthy donors is faster than the same reactions in the blood serum of donors with diseases. The results can be used in the diagnostics of the immune status and detection of chronic inflammation. We can recommend the dynamic light scattering method for implementation in biomedical diagnostics.

scholarly journals ВПЛИВ ІОНІВ СВИНЦЮ НА ІМУНОБІОЛОГІЧНІ ПОКАЗНИКИ У КУРЕЙ-НЕСУЧОК

2014 ◽  
pp. 197-199
Author(s):  
Н. Р. Серветник
Keyword(s):  
Body Weight ◽  
Immune Complexes ◽  
Lead Acetate ◽  
Laying Hens ◽  
Circulating Immune Complexes ◽  
Lead Ions ◽  
Research Groups ◽  
Immunological Parameters ◽  
The Impact ◽  
Different Doses

Представлено результати досліджень впливу зго-довування різних доз ацетату свинцю на імунобіоло-гічні показники курей-несучок. Встановлено, що занадходження в організм курей іонів свинцю в кілько-стях 2,5; 5 та 12,5 мг/кг маси тіла протягом 45 дібвідбувається вірогідне зростання концентрації сере-дньомолекулярних циркулюючих імунних комплексів усироватці крові птиці всіх дослідних груп порівняно зконтрольною. Також у курей-несучок дослідних групвідзначена тенденція до зростання ступеня ендоген-ної інтоксикації, про що свідчить збільшення в сиро-ватці крові вмісту молекул середньої маси. This paper presents the results of research on the impact of feeding different doses of lead acetate on immunological parameters of laying hens. Established that the insertion into the organism of chickens lead ions in amounts of 2.5, 5 and 12.5 mg / kg body weight for 45 days  caused a probable increase in the concentration of circulating immune complexes in the serum of birds of all experimental groups compared to controls. Also in laying hens research groups have observed a tendency to increase the degree of endogenous intoxication, as evidenced by an increase in serum content of medium molecules.

Sign in / Sign up

Export Citation Format

Share Document