scholarly journals Synthesis, Antibacterial and Antifungal Properties of Cyclohexane Tosyloxyimine Derivative

2019 ◽  
Vol 4 (3) ◽  
pp. 1-4
Author(s):  
Shoaib M
Keyword(s):  
Antimicrobial Agents ◽  
Sulfonyl Chloride ◽  
Antimicrobial Properties ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Bacteria And Fungi ◽  
Agar Well Diffusion Assay ◽  
Antibacterial And Antifungal ◽  
Candida Pseudotropicalis

Due to increasing antimicrobial resistance, functionally substituted cyclohexane derivatives are being explored as potential antimicrobial agents. Reaction of diethyl 4 - hydroxy - 6 - (hyd - roxyimino) - 4 - methyl - 2 - phenylcyclohexane - 1,3 - dicarboxylate with 4 - toluene sulfonyl chloride in boiling acetone in the presence of equimolar triethylamine resulted in formation of diethyl - 4 - hydroxy - 4 - methyl - 2 - phenyl - 6 - ((tosyloxy)imino) cyclohexane - 1,3 - dicarboxylate. The structure of novel compound was characterized by 1 H and 13 C NMR spectra and elemental analysis was performed. Agar well diffusion assay was used to screen novel compound against Gram - positive bacteria, Gram - negative bacteria and fungi. Test compound showed better antimicrobial properties against Gram - negative bac teria as compared to Gram - positive bacteria and fungi. Acinetobacter baumannii BDU - 32 was found to be most sensitive bacteria while Candida pseudotropicalis BDU MA88 was found to be most sensitive yeast.

Antimicrobial Properties of α-Dicarbonyl and Related Compounds

1983 ◽  
Vol 46 (4) ◽  
pp. 325-329 ◽  
Author(s):  
J. M. JAY ◽  
G. M. RIVERS ◽  
W. E. BOISVERT
Keyword(s):  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Broth Culture ◽  
Plate Count ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Plate Count Agar ◽  
Enzyme Substrate

By surface plating on plate count agar, 0.005 M (430 ppm) of the α-dicarbonyl compound, diacetyl, inhibited 28 of 40 organisms with the medium at pH 6 but only 11 at pH 8. Diacetyl was more effective against gram-negative bacteria and yeasts than non-lactic gram-positive bacteria and least effective against lactic acid bacteria. Acetoin, butanedioldiacetate and five butanediol isomers were considerably less effective than diacetyl, although all were more effective at pH 6 than 8. Diacetylmonoxime and diacetyldioxime were more effective than the diols and were less affected by pH of medium than diacetyl. Phenylglyoxal (PG) and 1,2-cyclohexanedione (CHD) ranked closest to diacetyl but, unlike the latter, they were more effective against gram-positive bacteria and less so against fungi and gram-negative bacteria. All 12 compounds were more effective against selected organisms by pour plating and in broth culture than by surface plating. Like diacetyl, CHD and PG are α, α-dicarbonyls and are widely used as arginine reactive agents resulting in the blockage of enzyme-substrate reactions. Although their antimicrobial activity may be due to this property, they apparently affect different enzymes than diacetyl because their antimicrobial spectra were different. The possibility of a class of food-use antimicrobial agents bearing α-dicarbonyl groups is suggested by the findings of this study.

scholarly journals Synthesis and Evaluation of Some Coumarin Based Schiff's Bases as Potential Antimicrobial Agents'

2012 ◽  
Vol 9 (4) ◽  
pp. 2079-2088 ◽  
Author(s):  
Vishakha Bansode ◽  
Meenakshi N. Deodhar
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
H Nmr ◽  
Nmr Data ◽  
Bacteria And Fungi

A series of the title compounds 3-(4-(4, 5-dihydro-5-(substituted phenyl)-1H-3-pyrazolyl) phenylimino) methyl)-4-chloro-2H-chromen-2-one 5(a-g) have been synthesized. These compounds were characterized on the basis of their spectral (IR,1H NMR) data and evaluated for antimicrobial activityin vitroagainst gram positive bacteria, gram negative bacteria and fungi. The compound (5b) was found to be the most active with MIC of 20 µg/ml against all the tested organisms.

scholarly journals The Carpathian lingonberry, raspberry and blackberry fruit extracts feature variable antimicrobial efficiency

2019 ◽  
pp. 27-32
Author(s):  
Emőke Mihok ◽  
Éva György ◽  
Endre Máthé
Keyword(s):  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Gram Positive ◽  
Gram Negative ◽  
Antimicrobial Effects ◽  
Fruit Extracts ◽  
Gram Positive Bacteria ◽  
Wild Berries

Wild berry is an excellent source of phytonutrients and/or bioactive compounds associated with significant therapeutic properties, so that they have been utilized in folk medicine and traditional nutrition throughout centuries. Multiple health-promoting effects, such as anti-inflammatory, anti-diabetic, anti-heart and coronary disease properties were attributed to such wild berries. It has also been proved that berries could feature antimicrobial effects that could be of a great importance for the prevention of food-feed poisoning and fighting back antibiotic resistance. In this study, we investigated the antimicrobial properties of lingonberry (Vaccinium vitis-idaea), raspberry (Rubus idaeus) and blackberry (Rubus fruticosus) crude and ethanolic extracts prepared from fruits obtained from the spontaneous flora of Eastern Carpathian Mountains situated in Transylvania. The antimicrobial effect of crude and alcoholic extracts were assessed on four Gram-negative, five Gram-positive bacteria and one yeast species using the agar diffusion method. The studied bacteria can cause food or feed spoilage and foodborne diseases. Our results indicate the significant inhibitory effect of lingonberry extracts in the case of Gram-negative bacteria like Proteus vulgaris and Salmonella Hartford, while among Gram-positive bacteria the strongest inhibitory effect was observed for Bacillus species like B. cereus, B. subtilis, B. mojavensis and Micrococcus luteus. The raspberry and blackberry extracts featured milder inhibitory effects in the case of the studied bacteria species. Furthermore, we have studied the crude or ethanolic extract combinations associated antimicrobial effects synergistic/additive or antagonistic properties. Interestingly, the triple and double ethanolic extract mixes had stronger antimicrobial properties, whereas the crude extract mixes showed relatively reduced effects, if any. Our results indicate that the antimicrobial activity of studied fruit extracts obtained from wild berries can vary upon the applied extraction method and their combination formulae, so that all these considerations must be taken into account when such fruit extracts are considered for foodstuff development.

scholarly journals Construction of Leaderless-Bacteriocin-Producing Bacteriophage Targeting E. coli and Neighboring Gram-Positive Pathogens

2021 ◽  
Author(s):  
Yoshimitsu Masuda ◽  
Shun Kawabata ◽  
Tatsuya Uedoi ◽  
Ken-ichi Honjoh ◽  
Takahisa Miyamoto
Keyword(s):  
Antimicrobial Agents ◽  
Host Strain ◽  
Phage Resistance ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
T7 Phage

We demonstrated that we could combine LLB and phage to construct promising novel antimicrobial agents, LLB-phage. The first LLB-phage, lnqQ -T7 phage, can control the growth of both the Gram-negative host strain and neighboring Gram-positive bacteria while preventing the emergence of phage resistance in the host strain.

scholarly journals Purification of Bioactive Peptide with Antimicrobial Properties Produced by Saccharomyces cerevisiae

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 324 ◽  
Author(s):  
Shayma Thyab Gddoa Al-sahlany ◽  
Ammar Altemimi ◽  
Alaa Al-Manhel ◽  
Alaa Niamah ◽  
Naoufal Lakhssassi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Antimicrobial Properties ◽  
Antibacterial Peptide ◽  
Inhibition Activity ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Viable Cells ◽  
Ph Range

A variety of organisms produce bioactive peptides that express inhibition activity against other organisms. Saccharomyces cerevisiae is considered the best example of a unicellular organism that is useful for studying peptide production. In this study, an antibacterial peptide was produced and isolated from Saccharomyces cerevisiae (Baker’s yeast) by an ultrafiltration process (two membranes with cut-offs of 2 and 10 kDa) and purified using the ÄKTA Pure 25 system. Antibacterial peptide activity was characterized and examined against four bacterial strains including Gram-positive and Gram-negative bacteria. The optimum condition for yeast growth and antibacterial peptide production against both Escherichia. coli and Klebsiella aerogenes was 25–30 °C within a 48 h period. The isolated peptide had a molecular weight of 9770 Da, was thermostable at 50–90 °C for 30 min, and tolerated a pH range of 5–7 at 4 °C and 25 °C during the first 24 h, making this isolated antibacterial peptides suitable for use in sterilization and thermal processes, which are very important aspect in food production. The isolated antibacterial peptide caused a rapid and steady decline in the number of viable cells from 2 to 2.3 log units of gram-negative strains and from 1.5 to 1.8 log units of gram-positive strains during 24 h of incubation. The isolated antibacterial peptide from Saccharomyces cerevisiae may present a potential biopreservative compound in the food industry exhibiting inhibition activity against gram-negative and gram-positive bacteria.

scholarly journals Synthesis of cyclotetrapeptide analogues of c-PLAI and evaluation of their antimicrobial properties

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Rani Maharani ◽  
Orin Inggriani Napitupulu ◽  
Jelang M. Dirgantara ◽  
Ace Tatang Hidayat ◽  
Dadan Sumiarsa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Properties ◽  
Trichophyton Mentagrophytes ◽  
Solution Phase ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Aromatic Residues ◽  
Gram Positive Bacteria ◽  
Lysine Residues ◽  
Bacteria And Fungi

Antimicrobial peptides (AMPs) are interesting compounds owing to their ability to kill several pathogens. In order to identify new AMPs, c-PLAI analogues were synthesized and evaluated together with their linear precursors for their antimicrobial properties against two Gram-positive bacteria ( Staphylococcus aureus and Bacillus cereus ), two Gram-negative bacteria ( Escherichia coli and Klebsiella pneumoniae ), and two fungal strains ( Candida albicans and Trichophyton mentagrophytes ). The new c-PLAI analogues were prepared through a combination of solid- and solution-phase syntheses, as previously employed for the synthesis of c-PLAI. The antimicrobial activity tests showed that the synthetic parent peptide c-PLAI was inactive or weakly active towards the bioindicators employed in the assay. The tests also indicated that cyclic c-PLAI analogues possessed enhanced antimicrobial properties against most of the bacteria and fungi tested. Furthermore, this study revealed that analogues containing cationic lysine residues displayed the highest activity towards most bioindicators. A combination of lysine and aromatic residues yielded analogues with broad-spectrum antimicrobial properties.

scholarly journals Synthesis, Characterization and Antimicrobial Studies of SnO2 Nanoparticles

2020 ◽  
Vol 13 (3) ◽  
pp. 203-209
Author(s):  
Sumathi . P
Keyword(s):  
Antibacterial Activity ◽  
Sno2 Nanoparticles ◽  
Antimicrobial Properties ◽  
Cost Effective ◽  
Salmonella Typhi ◽  
Gram Positive Bacteria ◽  
Antimicrobial Studies ◽  
Sno2 Nanoparticle ◽  
Bacteria And Fungi ◽  
Antibacterial And Antifungal

This study was involved to synthesize and investigate the antimicrobial properties of highly pure nanocrystalline SnO2 by simple chemical method. In the nanorods, SnO2 nanoparticles, with a size of about 74 nm, the SnO2 nanoparticles were maximum antibacterial activity against both bacterial and fungal with the zone of inhibition for Klebsiella pneumoniae, Staphylococcus aures, Salmonella typhi and Ascerpergillus Flavus, Ascerpergillus Niger from 25μg/ml and 100 μg/ml respectively. SnO2 nanoparticles showed good activity against both Gram-negative and Gram-positive bacteria confirming these as future broad spectrum antibacterial a cost effective way and to study its antimicrobial properties. We observed an effective antibacterial and antifungal activity of the SnO2 nanoparticle against bacteria and fungi. The results showed that SnO2 nanoparticles enhanced the good antibacterial activity.

scholarly journals Antimicrobial Biophotonic Treatment of Ampicillin-Resistant Pseudomonas aeruginosa with Hypericin and Ampicillin Cotreatment Followed by Orange Light

Pharmaceutics ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 641 ◽  
Author(s):  
Seemi Tasnim Alam ◽  
Tram Anh Ngoc Le ◽  
Jin-Soo Park ◽  
Hak Cheol Kwon ◽  
Kyungsu Kang
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Cell Wall ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Wall Structures ◽  
Global Issue ◽  
Life Threatening ◽  
Bacteria And Fungi

Bacterial antibiotic resistance is an alarming global issue that requires alternative antimicrobial methods to which there is no resistance. Antimicrobial photodynamic therapy (APDT) is a well-known method to combat this problem for many pathogens, especially Gram-positive bacteria and fungi. Hypericin and orange light APDT efficiently kill Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), and the yeast Candida albicans. Although Gram-positive bacteria and many fungi are readily killed with APDT, Gram-negative bacteria are difficult to kill due to their different cell wall structures. Pseudomonas aeruginosa is one of the most important opportunistic, life-threatening Gram-negative pathogens. However, it cannot be killed successfully by hypericin and orange light APDT. P. aeruginosa is ampicillin resistant, but we hypothesized that ampicillin could still damage the cell wall, which can promote photosensitizer uptake into Gram-negative cells. Using hypericin and ampicillin cotreatment followed by orange light, a significant reduction (3.4 log) in P. aeruginosa PAO1 was achieved. P. aeruginosa PAO1 inactivation and gut permeability improvement by APDT were successfully shown in a Caenorhabditis elegans model.

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