scholarly journals Uji Aktivitas Antijamur Gel Serbuk Lidah Buaya (Aloe vera L.) Berbasis Carbopol 934 Terhadap Candida albicans dan Trichophyton mentagrophytes

2019 ◽  
Vol 15 (2) ◽  
pp. 42-51
Author(s):  
Hani Afifah ◽  
Setyo Nurwaini
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Physical Properties ◽  
Fungal Infections ◽  
Aloe Vera ◽  
Trichophyton Mentagrophytes ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
High Concentration ◽  
Aloe Vera Gel

Fungal infections that occur in the skin and nails were caused by fungi such as Candida albicans and Trichophyton mentagrophytes. Aloe vera contains saponins and acemannan that were effective as an antifungal. This study aims to determine the effect of carbopol concentration variation on aloe vera gel to antifungal activity of Candida albicans and Trichophyton mentagrophytes.Aloe vera powder was obtained from freeze drying aloe vera juice which had been frozen. Aloe vera powder was formulated in gel form using carbopol base with concentration 0.5; 1.0; 1.5; and 2.0 %w/w. The aloe vera gel was tested for its physical properties and then tested the antifungal activity using the diffusion method of wells.The result of evaluation of physical properties showed the high concentration of carbopol then pH, viscosity, adhesiveness increased, and spreading capacity decreased. The results of antifungal activity test showed that aloe vera powder had activity on Candida albicans and Trichophyton mentagrophytes with inhibition zone of 15 ± 3 mm and 13 ± 0,5 mm. Aloe vera gel test results with variation of carbopol concentration 0.5; 1.0; 1.5; and 2.0 %w/w did not affect the antifungal activity of Candida albicans and Trichophyton mentagrophytes.

scholarly journals An evaluation of the antifungal activity of some local medicinal plants against growth of Candida albicans in vitro

2010 ◽  
Vol 9 (2) ◽  
pp. 60
Author(s):  
J.S. Al-Hussaini, and A. M. G. Al-Mohana
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Culture Media ◽  
Aloe Vera ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Elettaria Cardamomum ◽  
Local Plants

The aim of this study was to evaluate the antifungal activity of the ethanolic extract of three local plants ( Elettaria cardamomum, Aloe vera, Thyme Vulgaris) against the growth of pathogenic Candida albicans in culture media. The antifungal activity was carried out by using agar well diffusion method. Ethanolic extracts of Elettaria cardamomum and Aloe vera inhibited the growth of Candida albicans isolates at all concentrations which tested in the present study (25, 50, 100, 150, 200, and 400) mg/ml, while the extract of Thymus vulgaris showed no activity against tested Candida albicans

scholarly journals UJI AKTIVITAS ANTIJAMUR BEBERAPA SENYAWA MONOKARBONIL ANALOG CURCUMIN HASIL SINTESIS

2018 ◽  
Vol 5 (2) ◽  
pp. 149
Author(s):  
Ismi Rahmawati ◽  
Desi Purwaningsih
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Aspergillus Niger ◽  
1H Nmr ◽  
Aldol Condensation ◽  
Fungal Infections ◽  
Trichophyton Mentagrophytes ◽  
Curcumin Analogue ◽  
Pityrosporum Ovale ◽  
Post Hoc

Antifungal Activity of Some Synthesized Mono-Carbonyl Analogue Compounds of CurcuminABSTRACTFungal resistance can pose a threat to future fungal infections, therefore studies to find other compounds that have antifungal activity need to be done. The aim of this study was to examine antifungal activity of synthesized curcumin analogue compounds i.e. 2,6-Bis-(2'-furilidin)-cyclohexanone (26FuH); 2,5-Bis-(2'-furilidine)-cyclopentanone (25FuP) and 1,5-Difuril-1,4-pentadien-3-on (15FuA). The curcumin analogue compound was successfully synthesized with Aldol condensation using KOH 7.5% as the catalyst. The compound was purified and characterized by melting point, thin layer chromatography, gas chromatography with mass spectrometry, FTIR spectrophotometry, spectrophotometry 1H-NMR. The results showed pure compounds and have a structure that corresponds to the target compounds. All compound were assayed as antifungal against Candida albicans, Pityrosporum ovale, Aspergillus niger, and Trichophyton mentagrophytes. The activity of each compound represented by inhibitory diameter was analyzed by one-way ANOVA followed by post hoc Tukey (p<0.05). All three compounds showed antifungal activity against Candida albicans, Pityrosporum ovale, and Aspergillus niger. The best antifungal activity was shown by 26FuH against Pityrosporum ovale.Keywords: antifungal activity, curcumin, monocarbonyl, Pityrosporum ovale, synthesis ABSTRAKResistensi jamur dapat menjadi ancaman pada kasus infeksi jamur di masa mendatang, oleh sebab itu penelitian untuk menemukan senyawa lain yang memiliki aktivitas antijamur perlu dilakukan. Penelitian ini bertujuan untuk menguji aktivitas antijamur senyawa analog curcumin hasil sintesis yaitu senyawa 2,6-Bis-(2’-furilidin)-sikloheksanon (26FuH); 2,5-Bis-(2’-furilidin)-siklopentanon (25FuP) dan 1,5-Difuril-1,4-pentadien-3-on (15FuA). Senyawa analog curcumin sudah berhasil disintesis dengan metode kondensasi Aldol menggunakan katalis KOH 7,5%. Senyawa hasil sintesis dimurnikan dan dikarakterisasi dengan menggunakan pemeriksaan organoleptis, titik lebur, kromatografi lapis tipis, kromatografi gas dengan spektrometri massa, spektrofotometri FTIR, spektrofotometri 1H-NMR. Hasil menunjukkan senyawa murni dan struktur sesuai senyawa target. Hasil sintesis diuji aktivitas antijamur terhadap Candida albicans, Pityrosporum ovale, Aspergillus niger dan Trichophyton mentagrophytes. Hasil diameter daya hambat dianalisis dengan ANOVA satu arah dilanjutkan post hoc Tukey (p<0,05). Ketiga senyawa memiliki aktivitas antijamur terhadap jamur Candida albicans, Pityrosporum ovale, dan Aspergillus niger. Aktivitas antijamur terbaik adalah senyawa 26FuH terhadap jamur Pityrosporum ovale.Kata Kunci: aktivitas antijamur, curcumin, monokarbonil, Pityrosporum ovale, sintesis

scholarly journals FRAKSI ETANOL EKSTRAK KULIT DURIAN (Durio zibethinus L.) SEBAGAI ANTIFUNGI TERHADAP Trichophyton mentagrophytes DAN Candida albicans

1970 ◽  
pp. 28-38
Author(s):  
Yuli Wahyu Tri Mulyani ◽  
Subur Widodo ◽  
Lisa Selviani
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Trichophyton Mentagrophytes ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Bark Extract ◽  
Chromatography Method ◽  
Durio Zibethinus ◽  
Ethanol Fraction

Durian is a fruit that is in demand by the community, but during durian season environmental problems arise due to waste from skin of durian. The aimed of the study was to prove the antifungal activity of the ethanol fraction of durian skin against Trichophyton mentagrophytes and Candida albicans and to determine the active active compounds inhibiting T. mentagrophytes and C. albicans fungi. Durian skin was extracted by maceration method, then fractionated used ethanol, chloroform, and n-hexane solvents. Ethanol fraction was tested on T. mentagrophytes and C. albicans fungi used the disc method with a concentration of 10%, 20%, 40%, 60%, 80%, 100%, distilled water as a negative control, ketoconazole as a positive control. The best inhibition zone is fungi T. mentagrophytes concentration 100% ( 9.23 mm) negative control (0 mm), positive control (32.96 mm), fungi C. albicans the best inhibition zone is concentration of 100% (8.30 mm), positive control (17.17 mm) negative controls (0 mm).The ethanol fraction was screened by the Thin Layer Chromatography method and positively contained flavonoids (rf 0.7), tannins (rf 0.58), saponins (rf 0.76) and alkaloids (rf 0.64). Minimum Inhibitory Concentrations of T. mentagrophytes were 7% and C. albicans were 8%. The most effective active compound growht of T. mentagrophytes is tannins (rf 0.7) and for C. albicans is flavonoid (rf 0.6). The ethanol fraction of durian bark extract has antifungal activity against T. mentagrophytes and C. albicans. Keywords: Antifungi, Candida albicans, ethanol fraction skin of durian, Trichophytonmentagrophytes

Curcuma turmeric oil enhanced anti-dermatophytic drug activity against Candida albicans and Trichophyton mentagrophytes

2021 ◽  
Vol 18 ◽  
Author(s):  
Mahendra Singh ◽  
Alka Alka ◽  
Kyung Eun Lee ◽  
Pradeep Kumar ◽  
Sang Gu Kang
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Fungal Infections ◽  
Diffusion Mechanism ◽  
Trichophyton Mentagrophytes ◽  
World Health ◽  
World Population ◽  
Topical Cream ◽  
The World ◽  
Athlete’S Foot

Background: As per the World Health Organization survey, it has been found that dermatophyte infections are affecting around one-fourth of the world population. The dermatophytes are commonly keratinophilic in nature which can multiply and invade the keratinized tissues and affect various parts of the human body like nails, skin, and hair. The luliconazole is an antifungal drug utilized against dermatophytes which causes athlete's foot and ringworm etc. fungal infections of the skin or nails caused by Candida albicans (C.P. Robin) Berkhout and Trichophyton mentagrophytes (Robin) Blanchard. Objective: The study aimed to develop the luliconazole topical cream with turmeric oil and penetration enhancer to improve permeability and enhance antifungal activity. Methods: To prepare the luliconazole topical cream, various compositions of formulation were melted and mixed with varying concentrations of turmeric oil. The oil, drug, and aqueous phases were prepared separately and mixed stepwise in a vessel under continuous stirring at control conditions. Result: The optimized LC2 cream was showed pH 6.45±0.12, which is considered suitable to avoid irritation upon topical application. The LC2 cream formulation also showed significantly (p<0.05) more permeability with a permeation flux (0.347 mg/cm2/h) against an aqueous suspension of the drug (0.215 mg/cm2/h). The LC2 cream was followed the Higuchi model and showed the drug release from cream via a diffusion mechanism with super case II transport mechanism. Furthermore, the antifungal activity of optimized cream was found good than marketed cream. Conclusion: It is concluded that the prepared luliconazole cream can be an effective anti-fungal treatment with enhanced drug delivery into the skin to treat athlete's foot and ringworm etc. caused by dermatophytes namely C. albicans and Trichophyton spp.

scholarly journals Effectiveness of Ethanol Extract among Three Ganoderma Species From Lombok in Inhibition Growth of Candida albicans and Cryptoccocus neoformans

2020 ◽  
Vol 1 (1) ◽  
pp. 35
Author(s):  
Fitria Rizka Hidayat ◽  
Sukiman Sukiman ◽  
Ernin Hidayati ◽  
Sarkono Sarkono ◽  
Bambang Fajar Suryadi ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Large Diameter ◽  
Ethanol Extract ◽  
Pathogenic Fungi ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
High Concentration ◽  
Opportunistic Pathogenic

Candida albicans and Cryptoccocus neoformans are opportunistic pathogenic fungi that cause infectious diseases that are the world's biggest health problems. The use of antibiotics is one way to overcome the spread of the infection and cause microbial resistance. Ganoderma is one of the many macrophages found on Lombok's island, and studies of its antifungal activity have not been carried out. The purpose of this study was to determine the antifungal potential and the effect of different concentrations of ethanolic extracts of three Ganoderma species on C. albicans and C. neoformans. Ganoderma samples were obtained from Suranadi Taman Wisata Alam (TWA), Sesaot TWA, Tunak Mountain TWA, Kerandangan TWA, and Pusuk Forest. Ganoderma extraction was carried out by the maceration method using ethanol 95% solvent. The extract concentrations used are 20%, 40%, 60% and 80%. This research was conducted using the wells method with metronidazole as a positive control and 50% DMSO as a negative control. The parameter measured is the large diameter of the inhibition zone formed around the well. The results obtained are the three species of Ganoderma have antifungal activity against test fungi, and different levels of concentration affect inhibition. The amount of the inhibition zone is directly proportional to the high concentration of the extract. All three Ganoderma species are more effective in inhibiting the growth of C. neoformans compared with Candida albicans

Antifungal Activity of Peppermint Oil against Candida Albicans Isolated from Urine Samples

2019 ◽  
Vol 12 (4) ◽  
pp. 4611-4615
Author(s):  
Noura Berakda ◽  
Abdulkarim Radwan
Keyword(s):  
Candida Albicans ◽  
Urinary Tract ◽  
Antifungal Activity ◽  
Fungal Infections ◽  
In Vitro Study ◽  
Inhibition Zone ◽  
Antifungal Drugs ◽  
University Hospital ◽  
Peppermint Oil

Fungal infections with candida species are an important cause of morbidity and mortality. Situation is further worsened by increasing resistance to antifungal drugs. In this study, we sought to investigate the antifungal activity of peppermint oil against candida albicans of urinary tract candidiasis in females from Syria. An in vitro study was carried out using the following Candida albicans strains involved in urinary tract candidiasis using well diffusion (WD) testing: Candida albicans (ATCC 90028) and 15 strains were compiled from Aleppo university Hospital. It was taken from women having urinary tract candidiasis. The antifungal activity of peppermint oil was determined in the form of inhibition zone using antifungal assay agar WD testing. In all experiments, the obtained results indicated that peppermint oil has inhibitory effects on Candida albicans (ATCC 90028) and some 15 strains. This study showed that peppermint oil was active against the tested Candida albicans strains. Peppermint oil was more effective against Candida albicans compared to fluconazole. Peppermint oil may have potential for use in the development of clinically useful antifungal preparations. Therefore, peppermint oil might be highly effective in the natural prevention treatment of urinary tract candidiasis.

scholarly journals ANTIFUNGAL EXTRACT ACTIVITYOF ISOLATE Nocardia sp. ATS-4.1 AGAINST Candida albicans InaCC-Y116

2020 ◽  
Vol 19 (3A) ◽  
Author(s):  
Abdullah Abdullah ◽  
Rahmawati Rahmawati ◽  
Rikhsan Kurniatuhadi
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Fungal Infections ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Mueller Hinton ◽  
Nocardia Sp ◽  
Mueller Hinton Agar ◽  
Good Concentration

Candida albicans is a microorganism that knows as caused of candidiasis. Nocardia is known to have the ability to produce antifungal bioactive compounds to overcome cases of fungal infections. This research aims to determine the presence of antifungal activity and the good concentration from crude extract of Nocardia sp. ATS-4.1 to inhibit C. albicans InaCC-Y116 and. Antifungal activity test of isolate Nocardia sp. ATS-4.1 was performed using a well diffusion method on Mueller Hinton Agar (MHA) media with a concentration of 92%, 94%, 96%, 98%, 100% and nystatin (positive control) 0,0125% and DMSO 10% (negative control) incubated at 37 ᵒC for 24-48 hours. The results showed that the extract of Nocardia sp. ATS-4.1 can inhibit the growth of C. albicans. Nocardia sp. ATS-4.1 isolate extract concentration of 96% with a resistance diameter of 13.63 ± 0.53 mm with a strong category against the growth of  C. albicans InaCC-Y116.    

scholarly journals The Antifungal Activity of n-Hexane Extract of Eleutherine palmifolia (L). Merr Bulbs Against Candida albicans and Trichophyton mentagrophytes

2019 ◽  
Vol 7 (22) ◽  
pp. 3777-3780
Author(s):  
Masfria Masfria ◽  
Mitra S. A. Tampubolon
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Trichophyton Mentagrophytes ◽  
Ash Content ◽  
Hexane Extract ◽  
Water Soluble ◽  
Diffusion Method ◽  
Soluble Extract ◽  
Phytochemical Content ◽  
Extract Content

AIM: This research aimed to determine the characteristics of dried bulbs of Eleutherine palmifolia, the group of active chemical compounds contained in n-hexane extract of Eleutherine palmifolia bulbs and the antifungal activity of n-hexane extract of Eleutherine palmifolia bulbs against Candida albicans and Trichophyton mentagrophytes. METHODS: The Eleutherine palmifolia bulbs were extracted by percolation method using n-hexane solvent. The extract was tested for its antifungal activity against Candida albicans and Trichophyton mentagrophytes by diffusion method using paper discs. RESULTS: The characterisation of dried Eleutherine palmifolia bulbs were obtained water content 9.38%, water-soluble extract content 12.15%, ethanol-soluble extract content 14.48%, total ash content 0.91%, and acid insoluble ash content 0.70%. Determination of Phytochemical content showed alkaloid, flavonoid, tannin, saponin, glycoside, and steroid/triterpenoid compounds. The antifungal activity of Eleutherine palmifolia bulbs n-hexane extract (EPBHE) by concentration 200 mg/ml demonstrated the inhibition diameter of 19.48 and 42.20 mm for Candida albicans and Trichophyton metagrophytes, respectively. CONCLUSION: The antifungal test indicates that n-hexane extract of Eleutherine palmifolia bulbs provides inhibitory power to Candida albicans and Trichophyton mentagrophytes.

scholarly journals Antibacterial Activity of Aloe Vera Gel-based Edible Coating with the addition of Gum Arabic and Ascorbic Acid

2021 ◽  
Vol 5 (1) ◽  
pp. 1-4
Author(s):  
Ni Made Defy Janurianti ◽  
I Made Supartha Utama ◽  
Ida Bagus Wayan Gunam
Keyword(s):  
Antibacterial Activity ◽  
Aloe Vera ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Edible Coating ◽  
Diffusion Method ◽  
Heating Process ◽  
Antibacterial Compounds ◽  
E Coli ◽  
Aloe Vera Gel

Aloe vera gel has antibacterial properties. The content of antibacterial compounds in aloe vera gel is saponins, anthraquinones, tannins, aloin, and acemannan. Aloe vera gel has the potential as an edible coating for food products. The Processing carried out goes through a heating process that does think to damage the antibacterial compounds in the aloe vera gel. This study aims to determine the antibacterial activity of aloe vera gel formulated as an edible coating on S. aureus, S. mutans, E. coli and K. pneumoniae bacteria. The research method used is the disc diffusion method. Based on the test results, pure aloe vera gel has a larger inhibition zone diameter than aloe vera gel processed into an edible coating. Diameter of pure aloe vera gel inhibition zone in S. aureus, S. mutans, E. coli, and K. pneumoniae bacteria. respectively 10.966 ± 0.573 mm; 11.806 ± 0.215 mm; 10,860 ± 0.675 mm, and 10.686 ± 0.081 mm. Aloe vera gel formulated into an edible coating with antibacterial ability, namely at a concentration of 100%, which has anti activity with the inhibition zone's diameter in S. aureus, S. mutans E. coli, and K. pneumoniae bacteria, respectively. is 10,470 ± 0.213 mm; 10,673 ± 0.127 mm; 10,113 ± 0.040 mm, and 9.676 ± 0.604 mm.

AKTIVITAS ANTIJAMUR EKSTRAK ETANOL 50% DAN ETANOL 96% DAUN PACAR KUKU Lawsonia inermis L TERHADAP Trichophyton mentagrophytes

EKOLOGIA ◽  
2020 ◽  
Vol 19 (1) ◽  
pp. 12-19
Author(s):  
Oom Komala ◽  
Yulianita . ◽  
Fuji Raka Siwi
Keyword(s):  
Antifungal Activity ◽  
Ethanol Extract ◽  
Trichophyton Mentagrophytes ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Dilution Method ◽  
Disc Diffusion ◽  
Lawsonia Inermis ◽  
Disc Diffusion Method ◽  
Mic Value

Trichopyton mentagrophytes is a type of fungus that belongs to a group of dermatophytes, and a disease that causes dermatophytosis (ringworm), this fungus likes parts that contain keratin substances such as skin, hair / hair, nails or horns. This study aims to determine the antifungal activity of 50% and 96% ethanol extract. KHM testing was made with a concentration of 2.5%, 5%, 7.5% and 10% with the dilution method to determine KHM. Whereas in testing the width of the inhibitory area (LDH) was carried out after getting the MIC value at concentrations of 10%, 25% and 50% with disc diffusion method to determine the effective LDH and qualitative phytochemical testing. The results showed that 96% ethanol solvent at a concentration of 50% could inhibit the fungus Trichopyton mentagrophytes compared to 50% ethanol as seen from the inhibition zone formed in the width of the inhibitory area. Secondary metabolite compounds found in 50% ethanol extract and 96% ethanol are flavonoids, alkaloids, saponins, tannins, quinones and terpenoids.

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