scholarly journals LARVICIDAL AND ANTIFEEDANT ACTIVITIES OF CLOVE LEAF OIL AGAINST Spodoptera litura (F.) ON SOYBEAN

2020 ◽  
Vol 21 (1) ◽  
pp. 20-25
Author(s):  
Rizka Nur Fateha ◽  
Maria Grasela ◽  
Muhammad Nur Ichwan ◽  
Eny Wahyuning Purwanti ◽  
Irianti Kurniasari

Economic losses due to infestation by the soybean armyworm, Spodoptera litura can reach up to 100%. Some farmers often used insecticide to control the armyworm. However, regular use of insecticide can cause several negative effects such as environmental pollution, residual toxicity, and health hazard for human. The objective of this research was to determine the larvicidal, antifeedant, and activities of clove leaf oil (CLO) against the armyworm S. litura on soybean. The research was carried out at the plant pest control laboratory of Politeknik Pembangunan Pertanian Malang. Clove leaf oil was evaluated for its larvicidal and antifeedant activities against ten third instar larvae of the armyworm. Completely randomized design was used with concentration levels of CLO as treatments. i.e. 0.4, 0.5, 0.67, 1.0, and 2.0% by volume. Profenofos 500 g L 0.1% and distilled water were include as control. Each treatment consisted of five replications. Mortality of the larvae and antifeedant activity were analyzed using analysis of variance, followed by probit analysis for calculating LC 50 and LT 50 -1. The results showed that CLO at 2.0% concentration was the most effective with regard to larvicidal and antifeedant activities. The LC 50 of CLO was 0.09% with LT 24.6 hours. This study revealed that the environmentally friendly CLO can be used as an alternative to synthetic insecticide for control S. litura.

2020 ◽  
Vol 21 (1) ◽  
pp. 20-25
Author(s):  
Rizka Nur Fateha ◽  
Maria Grasela ◽  
Muhammad Nur Ichwan ◽  
Eny Wahyuning Purwanti ◽  
Irianti Kurniasari

Larvicidal and antifeedant activities of clove leaf oil against Spodoptera litura (F.) on Soybean. Economic losses due toinfestation by the soybean armyworm, Spodoptera litura can reach up to 100%. Some farmers often used insecticide to control thearmyworm. However, regular use of insecticide can cause several negative effects such as environmental pollution, residual toxicity,and health hazard for human. The objective of this research was to determine the larvicidal, antifeedant, and activities of cloveleaf oil (CLO) against the armyworm S. litura on soybean. The research was carried out at the plant pest control laboratory ofPoliteknik Pembangunan Pertanian Malang. Clove leaf oil was evaluated for its larvicidal and antifeedant activities against tenthird instar larvae of the armyworm. Completely randomized design was used with concentration levels of CLO as treatments.i.e. 0.4, 0.5, 0.67, 1.0, and 2.0% by volume. Profenofos 500 g L 0.1% and distilled water were include as control. Each treatment consisted of five replications. Mortality of the larvae and antifeedant activity were analyzed using analysis of variance, followed by probit analysis for calculating LC 50  and LT 50-1. The results showed that CLO at 2.0% concentration was the most effective with regard to larvicidal and antifeedant activities. The LC 50 of CLO was 0.09% with LT 24.6 hours. This study revealed that the environmentally friendly CLO can be used as an alternative to synthetic insecticide for control S. litura.


2021 ◽  
Vol 3 (1) ◽  
pp. 130-135
Author(s):  
Hapsoh ◽  
Desita Salbiah ◽  
Isna Rahma Dini ◽  
Johanna Yosefin BR Tobing

Red chili is a horticultural crop that is widely cultivated commercially. The need for red chili is increasing, in accordance with the increasing variety of types of food that use red chili as a raw material. Pest attacks on red chili plants are an obstacle in red chili cultivation. Spodoptera litura is a red chili pest that can cause yield reductions of up to 80%. Control using the Riau local entomopathogenic fungi Beauveria bassiana is an effort to control S. litura using environmentally friendly materials. This study aims to determine the effect of the application of the Riau local entomopathogenic fungi B. bassiana to contol S. litura pests and the growth of red chili plants. The research was carried out at the Plant Pest Laboratory and Experimental Garden, Faculty of Agriculture, Riau University from July to August 2020. This study was designed in a completely randomized design with five treatments, namely the concentration of Riau local Riau B. bassiana 20 gl-1 water, 25 gl-1 water, 30 gl-1 water, 35 gl-1 water and 40 gl-1 water and four replications to obtain 20 experimental units. The results showed that the concentration of Riau local B. bassiana 30 gl-1 of water containing a conidia density of 4.1 × 108 con.ml-1 was a concentration that affected S. litura pests and chili plant growth by causing the intensity of S. litura pests which could be categorized as lower at 4.15% and can cause a peak in larval death on the fourth day after application.


EKOLOGIA ◽  
2020 ◽  
Vol 18 (2) ◽  
pp. 55-62
Author(s):  
Rangga Eka S. P ◽  
Moerfiah . ◽  
Triastinurmiatiningsih .

The purpose of this study was to determine the activity of the leaf extract of Piper sarmentosum on mortality and damage levels by pest armyworm (Spodoptera litura). This research was conducted at the Laboratory of Biology, Faculty of Mathematics and Natural Sciences, Pakuan University, Bogor. This study uses a completely randomized design (CRD), with four levels of extract concentration, that is 0% as control, 30%, 40% and 50%.  Each treatment was repeated four times. The variables measured were mortality rates, the extent of damage the leaves, and larval growth. A concentration of EDK 50% showed activity in killing the armyworm by 38%. The lowest level of damage is 5%, with an average growth rate to its lowest larvae by 0.8 cm with the provision of treatment EDK 50%, and the higest level of damage is 75% by a concetration of EDK 30% treatments.


2017 ◽  
Vol 17 (2) ◽  
Author(s):  
I Made Indra Agastya ◽  
Aminudin Afandhi ◽  
Luqman Qurata Aini

This research was conducted in vitro in the laboratory. Studies conducted in the Laboratory of Bacteriology Department of Plant Pests and Diseases of the Faculty of Agriculture, University of Brawijaya, on the effectiveness of the bacteria Bacillus cereus and Bacillus megaterium as biological pesticides controlling Spodoptera litura. The objective of this study was to determine the effectiveness of the bacteria Bacillus sp as biological control. Effectiveness pesticide was measured by testing the incubation period and mortality in larvae of S. litura instar 3. This study used a completely randomized design (CRD), conducted observations every 6 hours until the larvae dead. The results showed that the percentage of mortality of S. litura reached 94.66% compared to the control, while the incubation time of the bacteria B. cereus cause disease until 29.84 hours. B. cereus and B. megaterium have the ability to incubate third instar larvae of S. litura up to 29.84 hours and caused the death of larvae up to 94.66%. Keywords: Biological pesticides, Bacillus cereus, Bacillus megaterium, entomopatogen, Spodoptera litura


2019 ◽  
Vol 4 (1) ◽  
pp. 28
Author(s):  
Kartina Kartina ◽  
Shulkipli Shulkipli ◽  
Mardhiana Mardhiana ◽  
Saat Egra

<h1>Spodoptera litura F. is one of the important pests in Horticulture commodities which can cause damage 80% and up to 100%. Karamunting (Melastome malabathricum) is a wild plant that grows in Tarakan City. This plant is known to contain secondary metabolites which are thought to have the potential to control pests. This study aims to determine the potential of Karamunting extract as a botanical insecticide material to control armyworms. This study uses a completely randomized design (CRD) method with 1 treatment factor, namely the concentration of Karamunting leaf extract. Potential extracts as botanical insecticides were tested by dye method using 4 concentrations, namely 0 (control); 500; 1000; 2000; 4000; and 8000 ppm. The results showed that Karamunting leaf extract was able to control armyworm pests. Giving extracts of 8000 ppm (P5) in feed caused the highest decrease in eating activity up to 41,2%, larval mortality up to 85%, and the highest mortality rate after 7 days of observation. Decreased feeding activity and larval mortality were thought to be influenced by the presence of phenol compounds, fatty acids, terpenoids, sterols and alkaloids identified in the extract. Thus, Karamunting leaf extract has the potential to be developed as an active ingredient in botanical insecticides to control armyworm.<strong></strong></h1>


Pro-Life ◽  
2019 ◽  
Vol 6 (1) ◽  
pp. 44
Author(s):  
Aseptianova Aseptianova

Mosquito Culex sp. L. is a vector of elephantiasis disease, prevention effort of elephantiasis vector is usually done by using chemical and natural insecticide. In the leaves of turmeric can be used as a natural insecticide in the prevention of elephantiasis vector disease. The objectives of this research are (1) to know the effect of turmeric extract ((Curcuma longa Linn.) As electrical insecticide to mortality of Culex sp. L. (2) To know the concentration of turmeric extract (Curcuma longa Linn.) Which is effective to be used as an electric insecticide against mortality of Culex sp. L. expressed by the toxicity test of LC50 and LT50. The method used in this research is an experimental method with Completely Randomized Design (RAL) which consists of 5 treatments and 4 replications. Based on research result of turmeric extract (Curcuma longa Linn.) Have a very significant effect on mortality of Culex sp. L. with sig value 0,000 <sig value. 0.05. The probit analysis of LC50 of turmeric leaves extract on mortality of Culex sp. L. is 44, 840 ml, meaning that at that concentration has occurred 50% mortality of total mosquito. The probit analysis of LT50 turmeric leaves extract on mortality of Culex sp. L. for 1.825 hours.   Keywords: turmeric leaves extract, mortality Culex sp. L. mosquito


2010 ◽  
Vol 10 (2) ◽  
pp. 154-161
Author(s):  
Effendy TA ◽  
Robby Septiadi ◽  
Abdullah Salim ◽  
Abdul Mazid

Entomopathogenic fungi from the lowland soil of South Sumatera and their potential as biocontrol agents of Stink Bug (Leptocorisa oratorius (F.)). The purpose of this research was to explore and examine the potential of soil borne entomopathogenic fungi from South Sumatera as biological agents of Leptocorisa oratorius (F).  Soil used for trapping entomopatogenic fungi, was taken from five locations  in South Sumatera.  Percentage of nymph mortality was analyzed by using analysis of variance and arranged in Completely Randomized Design.  LT50­ of nymph mortality was determined with Probit Analysis.  Entomopathogenic fungi found in this research were Beauveria bassiana and Metarhizium sp.  Stink bug mortality due to B. bassiana isolates reached 40-73.3% and Metarhizium sp. reached 56.7-70%. The results showed that Metarhizium isolates were more virulent than B. bassiana isolates.  The viability of B. bassiana conidia did not decrease during sub-culturing.  Sub-culturing did not influence the mortality of stink bug nymph but it prolonged the LT50­  of the stink bug.


2018 ◽  
Vol 7 (1) ◽  
pp. 1-8 ◽  
Author(s):  
A. V. Amalia ◽  
M. H. Yusa

The increasing of pesticide use is in line with the increasing number of pest populations. However, the use of pesticides causes various negative impacts on the environment (soil, water, and air) such as pesticide-resistant pests, perishing of useful insects which are non-target pesticides, and the use of pesticide which can even lead to poisoning and death in humans. One of the environmentally-safe techniques to control pests is chemical, by the use soursop leaf extract. This study aims to determine the most effective concentration of soursop leaf extract (Annona muricata) in controlling leaf caterpillar (Plutella xylostella) in delima rose apples. The research was carried out in the plantation area of rose apples in tempuran village, Demak. The research method employed experimental research. The study was conducted using Completely Randomized Design. The data were obtained using probit analysis of LC50. The most effective concentration of soursop extract (Annona muricata) on leaf caterpillars’ mortality was 75 ppm. Soursop leaf extract (Annona muricata) has been proven to influence rose apples leaf caterpillars’ mortality index. The LC50 value of soursop leaf extract was 75,16 mg/L. The result showed that soursop leaf extract (Annona muricata) is effective as a biopesticide material to control leaf caterpillar pest for delima rose apples.


Author(s):  
Rusli Rustam ◽  
Agus Sutikno ◽  
Jamiatul Laila

Rice is one of serealea comodity that susceptible with pest storage. One of the pest that often attack the rice in storage is Sitophilus oryzae L. The alternative for control S. oryzae L. as fumigant which enviromentally sound is Gliricidia sepium Jacq. as organic insecticide. This research aims to get the best dose G. sepium Jacq. leaf powder to control S. oryzae L. pest in rice storage. This research conducted at Laboratory of Plant Pest, Faculty of Agriculture, University of Riau, Pekan baru from Mei until Juni 2016. This research arranged experimentally by using Completely Randomized Design consisting of 5 treatments and 4 replications. The treatment consist of 5 dose levels were 0 g/100 g rice, 2 g/100 g rice, 4 g/100 g rice, 6 g/100 g rice and 8 g/100 g rice. The result showed that giving of G. sepium Jacq. leaf powder with dose 8 g/100 g rice causes time of death beginningS. oryzae L. during 31.50 hours after application, lethal time 50 during 282.8 hours after application, total mortality was 70%, amount of generation was 9,75 beetles and  decrease of seed weight’s rice was 1.08%.


2020 ◽  
Vol 1 (2) ◽  
pp. 55
Author(s):  
Laily Dwi Dzulhijja ◽  
Wagiyana W ◽  
Sigit Prastowo

This study aims to determine the effect of soursop leaf flour, lemongrass flour, kenikir leaf flour, lime leaf flour, and kluwih leaf flour on: 1) mortality, 2) damage to seeds, 3) shrinkage of seed weight, 4) appearance of adult C. analysts on soybean seeds in deposits and 5) laying eggs of C. analis imago female eggs, 6) probit analysis LT50. The experiment was carried out using a Completely Randomized Design (CRD). As treatments are: A) controls; B) soursop leaf flour in doses of 1 g, 2 g, and 3 g; C) Serai leaf flour with doses of 1 g, 2 g, and 3 g; D) kenikir leaf flour with doses of 1 g, 2 g, and 3 g; E) lime leaf flour with doses of 1 g, 2 g, and 3 g; and F) kluwih leaf flour with doses of 1 g, 2 g, and 3 g. Each treatment uses five replications. The observed parameters were analyzed by "F" test variance then if it was significantly different to find out the difference in the effect of treatment, an Tukey HSD Test was conducted at a level of 5% distrust. The best mortality results using the treatment of soursop leaf flour dose 3 g on 5 days amounted to 1.80 tails; 1 month is 6.60; 2 months totaling 13.40; and 3 months totaling 11.20. The results of the observation of the number of eggs produced by C. analis the best analyst was the treatment of soursop leaf flour dose 3 g on 5 days amounting to 139 items, 1 month 119.20 items; 2 months 91.20 items; and 3 months 72.20 items. The best imago observation results were treatment of soursop leaf flour dose 3 g at 1 month amounting to 132.20 tails; while at 2 months there were 79 heads. The best observation of seed damage and shrinkage of seed weight was the treatment of soursop leaf flour with a dose of 3 g, in the damage of seeds of 67.40 grains of seed damage; while the seed weight is reduced by 17.94 g. The results of the best analysis of Probit LT50 were treatment of soursop leaf flour with a dose of 3 g because it could kill 50% of the population C. analis with a relatively short time were 9 days after treatment.


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