scholarly journals INNOVATION OF SINBIOTIC FORMULA FOR THE GROWTH OF WHITE SHRIMP LARVAE (Litopenaeus vannamei)

2021 ◽  
Vol 7 (2) ◽  
pp. 71-81
Author(s):  
Sumardi Sumardi ◽  
Christina Nugroho Ekowati ◽  
Endang Linirin Widiastuti ◽  
Ainun Rohmawati Bareta ◽  
Sarno Sarno
Keyword(s):  
Water Quality ◽  
Survival Rate ◽  
Litopenaeus Vannamei ◽  
Sea Water ◽  
Positive Control ◽  
Negative Control ◽  
National Standard ◽  
White Shrimp ◽  
Length Growth ◽  
Absolute Length

In the hatchery of white shrimp larvae, there are some problems, such as a decreased water quality and diseases caused by bacteria which cause a decrease in shrimp growth. The solution to reduce these problems is by applying synbiotics. This research aimed to make an innovative synbiotic formula for the absolute length growth and survival rate of white shrimp larvae (Litopenaeus vannamei) as well as  the total number of bacteria and the number of Vibrio sp. In this study, there were four treatments, as follows: C-: negative control (without synbiotic application) C+: positive control (commercial synbiotic) P: probiotic 2.5 x 105 cfu/ml and prebiotic 1,5 ppm Q: probiotic 5 x 105 cfu/ml and prebiotic 1 ppm R: probiotic 7,5 x 105 cfu/ml and prebiotic 0,5 ppm S: probiotic 106 cfu/ml and prebiotic 2 ppm, each treatment with four repetitions. This research was set up in a completely randomized design experiment using twenty-four plastic tanks with 50 L total volume filled with 20 L sterile sea water and stocked with 4,000 nauplii in the PT. Citra Larva Cemerlang hatchery, Kalianda, Lampung. Variables observed in this research were survival rate, absolute length growth, total bacterial and Vibrio sp. counts, and water quality in the white shrimp larvae (Litopenaeus vannamei). The results showed that the best survival rate is in treatment S (probiotic 106 cfu/ml and prebiotic 2 ppm) (87.7%), the highest absolute length growth is in treatment P (3.8 mm), the highest total bacteria was in treatment S (11.1 log cfu/ml), and the best total Vibrio sp. is in treatment S (3.5 log cfu/ml). Water quality of the six treatments shows results that are still in normal conditions following Indonesian National Standard SNI 7311: 2009.

scholarly journals APLIKASI SINBIOTIK UNTUK PENCEGAHAN INFEKSI INFECTIOUS MYONECROSIS VIRUS PADA UDANG VANAME (Litopenaeus vannamei) (Synbiotic Application for Prevention of Infectious Myonecrosis Virus Infection in White Shrimp (Litopenaeus vannamei))

2016 ◽  
Vol 10 (2) ◽  
pp. 121-127
Author(s):  
Widanarni Widanarni ◽  
Sukenda Sukenda ◽  
Ghita Ryan Septiani
Keyword(s):  
Survival Rate ◽  
Immune Responses ◽  
Litopenaeus Vannamei ◽  
Ipomoea Batatas ◽  
Challenge Test ◽  
Positive Control ◽  
Negative Control ◽  
White Shrimp ◽  
Infectious Myonecrosis Virus ◽  
Synbiotic Supplementation

This study aimed to evaluate the effectiveness of dietary synbiotic at different giving frequencies on growth, immune responses, and resistance of white shrimp infected by infectious myonecrosis virus (IMNV). Synbiotic used in this study was combination of probiotic Vibrio alginolyticus SKT-b and prebiotic oligosaccharides extracted from sweet potatoe (Ipomoea batatas L). Doses of probiotic and prebiotic used were 1% and 2% (w/w), respectively. The white shrimps (0.493±0.035 g) were divided into five treatments consisting of A and B (without supplementation of synbiotic: (A) positive control; (B) negative control), C (daily synbiotic supplementation), D (twice a week synbiotic supplementation), and E (weekly synbiotic supplementation). After 30 days of feeding trial, white shrimps were infected by IMNV (except negative control). The results showed that daily growth rate of white shrimp on all synbiotic treatments (C, D, and E) ranged from 6.93±0.025-6.97±0.019% and had higher values than controls (A and B) (P<0.05). Meanwhile, feed conversion value in C and D (1.54±0.142 and 1.58±0.117) were lower than controls (P<0.05). Supplementation of synbiotic with different frequencies also affected survival rate of white shrimp after the challenge test with IMNV; daily synbiotic supplementation (C) resulted in a 50% higher survival rate than positive control (P<0.05). This was associated with immune responses parameters values of synbiotic treatment (before and after the challenge test) which were better than positive control. In conclusion the addition of synbiotic in feed resulted in higher growth performances, immune responses,and resistance of white shrimp to IMNV infection.

scholarly journals MASCULINIZATION OF NILE TILAPIA (Oreochromis niloticus) USING JAVA LONG PEPPER (Piper retrofractum) EXTRACT THROUGH LARVAL IMMERSION

2019 ◽  
Vol 4 (2) ◽  
pp. 29-34
Author(s):  
Hafizhotur Rohmaniah ◽  
Denny Syaputra ◽  
Ahmad Fahrul Syarif
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Nile Tilapia ◽  
Positive Control ◽  
Negative Control ◽  
Absolute Weight ◽  
Length Growth ◽  
Weight Growth ◽  
Randomized Design ◽  
Absolute Length

The aimed of this study was to evaluate the effectiveness of java long pepper extract in the increasing of the percentage of males nile tilapia. The design of experiment was completely randomized design with 5 treatments i.e : A (negative control), B (1,99 mg L-1), C (3,99 mg L-1), D (5,99 mg L-1) and positive control (20 mg L-1 17α-metiltestosteron) with three replicates per level of treatment. Immersion of 10-day-old larvae for 24 hours with 50 larvae in 10 L medium of treatments and cultivated for 60 days and identification of gender using the acetocarmin method. The results showed that java long pepper extract was effective to increase the percentage of males with dose of 1,99 mg L-1 (81,78±6,81%), 3,99 mg L-1 (79,00±2,99%) and 5,99 mg L-1 (87,42±4,85%) while the negative control 39,23±7,74%. Java long pepper extract dose of 5,99 mg L-1 were not significantly different with the treatment of 17α-metiltestosteron i.e : 91,71±3,93%. Percentage of survival rate 47.33±3.06% to 53.33±8.08%, specific growth rate 3,14±0,15% to 3,40±0,08%, absolute weight growth 1.97±0.59 g to 3.07±0.38 g and absolute length growth of 3.73±0.55 cm to 3.95±0.16 cm. Java long pepper extract gives a significant effects to the spesific growth  rate and absolute weight growth. Treatment A, B, C, D and E had no effect on survival rate and the absolute length growth of nile tilapia during 60 days cultivation period.

scholarly journals Administration of Phyllanthus niruri to control IMNV (myonecrosis infectious virus) infection white shrimp Litopenaeus vannamei

2015 ◽  
Vol 10 (2) ◽  
pp. 192
Author(s):  
Sukenda . ◽  
Sri Nuryati ◽  
Isni Rahmatika Sari
Keyword(s):  
Survival Rate ◽  
Litopenaeus Vannamei ◽  
Clinical Signs ◽  
Negative Control ◽  
Shrimp Farming ◽  
White Shrimp ◽  
Striated Muscles ◽  
Phyllanthus Niruri ◽  
Infectious Myonecrosis Virus ◽  
Shrimp Tissue

<p>ABSTRACT</p><p><br />Infectious myonecrosis (IMN) disease is a major disease in Indonesia shrimp farming. The disease is caused by infectious myonecrosis virus (IMNV). Currently, treatment and drug has not been obtained to control the virus. This research was conducted to determine the effect of Phyllanthus niruri extract in white shrimp (Litopenaeus vannamei) against IMNV infection. Healthy shrimp was given P. niruri extract 20 mg/kg of feed for seven days and after that the shrimp was challenged by orally with IMNV infected shrimp tissue. The positive control was given feed without P. niruri extract and challenged with IMNV infected shrimp tissue, while negative control was not challenged with IMNV infected shrimp tissue. IMNV infection gave a significantly different effect on survival rate. In the shrimp P. niruri previously (86.7%) gave higher survival rate compared to shrimp without P. niruri (66.67%). Survival rate of negative control was 93.33%. IMNV clinical signs in general was white necrotic areas in striated muscles. Histological examination showed that cell necrosis appeared on the mussel tissues. In conclusion the addition of P. niruri to the commercial feed can give the survival rate of shrimp better when challenged with IMNV.<br />Keywords: IMNV, Phyllanthus niruri, Litopenaeus vannamei</p><p><br />ABSTRAK</p><p><br />Penyakit infectious myonecrosis (IMN) merupakan penyakit utama pada budidaya udang di Indonesia. Penyakit ini disebabkan oleh infectious myonecrosis virus (IMNV). Saat ini, belum diperoleh cara dan obat untuk mengendalikan virus IMNV. Penelitian ini dilakukan untuk mengevaluasi pengaruh immunostimulan tepung meniran (Phyllanthus niruri) yang diberikan melalui pakan pada udang vaname (Litopenaeus vannamei) yang diinfeksi IMNV. Udang vaname yang sehat diberi pakan yang mengandung meniran dengan dosis 20 mg/kg pakan selama tujuh hari dan kemudian diuji tantang secara oral dengan memberikan jaringan udang yang telah terinfeksi IMNV. Udang kontrol positif dilakukan dengan memberi pakan komersial tanpa penambahan meniran yang kemudian diuji tantang dengan memberi makan jaringan udang yang terinfeksi IMNV, sedangkan udang kontrol negatif tidak diuji tantang dengan jaringan yang terinfeksi IMNV. Hasil menunjukkan bahwa kelompok udang yang diberi pakan mengandung meniran mempunyai kelangsungan hidup (86,67%) lebih tinggi dibandingkan dengan udang yang tidak diberi pakan mengandung meniran (66,67%) ketika diuji tantang dengan IMNV. Kontrol negatif yang tidak diberi pakan mengandung meniran dan tidak diuji tantang dengan IMNV memberikan kelangsungan hidup 93,33%. Gejala klinis yang ditunjukkan adanya infeksi IMNV terlihat dengan adanya otot putih pada ruas tubuh udang. Pengamatan histopatologi menunjukkan adanya nekrosis pada sel-sel di jaringan otot udang. Sebagai kesimpulan dapat dinyatakan bahwa penambahan meniran pada pakan komersial dapat meningkatkan kelangsungan hidup udang ketika terjadi infeksi IMNV.<br /><br />Kata kunci: IMNV, Phyllanthus niruri, Litopenaeus vannamei</p>

scholarly journals Use of Chitosan to Control Vibrio harveyi Infection on White Shrimp Litopeneaus vannamei

2007 ◽  
Vol 6 (2) ◽  
pp. 205
Author(s):  
. Sukenda ◽  
Y. Tri Anggoro ◽  
D. Wahjuningrum ◽  
. Rahman
Keyword(s):  
Antibacterial Activity ◽  
Survival Rate ◽  
Litopenaeus Vannamei ◽  
Antibacterial Effect ◽  
Sea Water ◽  
Vibrio Harveyi ◽  
White Shrimp ◽  
Phagocytic Index ◽  
Post Injection ◽  
Total Haemocyte Count

<p>Immunostimulation and antibacterial effect of chitosan against vibriosis were examined in white shrimp (<em>Litopenaeus vannamei</em>).  Control shrimps were injected with 0.05 μl of sterile sea water, while experimental shrimps were injected with 2, 4 and 6 μg per g shrimp of chitosan.  All shrimps were subsequently challenged by 10<sup>6</sup> CFU/ml of live <em>Vibrio harveyi</em> by injection method.  Survival rate of shrimps injected with chitosan were found to slightly increase in accordance with dose of chitosan, even not statistically significant.  Total haemocyte count and phagocytic index at experimental shrimps were over than control shrimps up to three days post injection.  Number of <em>V. harveyi</em> in the intestine of experimental shrimps were lower than control shrimps indicates an antibacterial activity of chitosan to combat infection.</p> <p>Keywords: chitosan,<em> </em><em>Vibrio harveyi</em>, haemocyte, phagocytic index, <em>Litopenaeus vannamei</em></p> <p> </p> <p>ABSTRAK</p> <p>Efek imunostimulasi dan antibakterial dari kitosan melawan vibriosis dilihat pada udang putih (<em>Litopenaeus vannamei</em>).   Udang control disuntik dengan 0,05 μl air laut steril, sedangkan udang uji disuntik dengan kitosan 2, 4 dan 6 μg per g udang.  Semua udang diuji tantang dengan 10<sup>6</sup> CFU/ml bakteri <em>Vibrio harveyi</em> hidup dengan metode penyuntikan.  Sintasan udang yang disuntik dengan kitosan meningkat berbarengan dengan peningkatan dosis kitosan, meskipun tidak menunjukkan perbedaan yang nyata.  Jumlah total hemosit dan indeks fagositosis pada udang lebih tinggi dibandingkan kontrol sampai tiga hari pasca penyuntikan.  Jumlah <em>V. harveyi</em> dalam saluran pencernaan dari udang uji lebih rendah dibandingkan udang kontrol, hal ini  menunjukkan aktifitas antibacterial dari kitosan dalam melawan infeksi.</p> <p>Kata kunci: kitosan,<em> Vibrio harveyi</em>, hemosit, indeks fagositosis, <em>Litopenaeus vannamei</em></p>

scholarly journals The Addition of Red Betel Leaf Extract (Piper crocatum) in the Feed of Vannamei Shrimps (Litopenaeus vannamei) for Vibriosis Prevention

2021 ◽  
Vol 10 (3) ◽  
pp. 365
Author(s):  
Fariq Azhar ◽  
Muhammad Junaidi ◽  
Bagus Dwi Hari Setyono ◽  
Andre Rachmat
Keyword(s):  
Survival Rate ◽  
Bacterial Infection ◽  
Litopenaeus Vannamei ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Betel Leaf ◽  
Shrimp Feed ◽  
Number Of Bacteria ◽  
Total Bacteria

Vibriosis is a type of shrimp disease caused by Vibrio sp. In the disease control of consumption biota, it is highly recommended to use natural ingredients that are not carcinogenic, one of which is red betel leaves (Piper crocatum). This study aims to determine the best dose of red betel leaf extract (P. crocatum) mixed in Vannamei shrimp feed to prevent vibriosis. In this study, shrimps were reared for 40 days at a density of 20 fish/container. The treatments included positive control, P1 (without extract + bacterial infection), negative control P2 (without extract and without bacterial infection), P3 (0.5% extract + bacterial infection), P4 (1% extract + infection), and P5 (2% extract + bacterial infection). The application of red betel leaf extract at a dose of 0.5% resulted in 75% survival after infection with V. parahaemolyticus, Vannamei shrimps of THC 7.70×106 cells/mL, and DHC (hyaline 82.94% granular 20.10%). The number of bacteria and the number of vibrio in the intestine were 52×108 CFU/mL and 12×108 CFU/mL accordingly. The best dose was obtained at P3 (Feed +0.5% red betel leaf extract), seen from the increase in survival rate, the number of hemocytes, differential haemocyte counts, and a decrease in total bacteria, so the application of red betel leaf extract can be used in the cultivation of Vannamei shrimps as an immunostimulant.

scholarly journals Oral application of probiotic, prebiotic, and synbiotic in Pacific white shrimp(Litopenaeus vannamei)challenged with Vibrio harveyi

2013 ◽  
Vol 11 (1) ◽  
pp. 54 ◽  
Author(s):  
. Widanarni ◽  
Puguh Widagdo ◽  
Dinamella Wahjuningrum
Keyword(s):  
Survival Rate ◽  
Vibrio Harveyi ◽  
Positive Control ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Control Treatment ◽  
Resistant Bacteria ◽  
Average Weight ◽  
High Survival ◽  
Immersion Method

<p>The use of antibiotics for controlling of luminous vibriosis caused by <em>Vibrio harveyi</em> is restricted now, because it induces antibiotic-resistant bacteria and leave residue in shrimp’s body. An alternative solution that can be done to treat the disease is by using applications of probiotic, prebiotic, and synbiotic. The aim of this research was to examine the effect of probiotic, prebiotic, and synbiotic on the survival rate and growth of Pacific white shrimp against <em>V. harveyi</em> infection. Feed as a treatment was supplemented with probiotic 1%, prebiotic 2%, and probiotic 1%+prebiotic 2% (synbiotic). Shrimps feed without supplementation of probiotic and prebiotic was used as a control treatment. The shrimps were maintainedin the aquarium (60×30×35 cm<sup>3</sup>) with a density of 40 shrimps/40 L and an average weight of 0.4±0.1 g. After 30 days of feeding treatment, the shrimp was challenged by immersion method with <em>V. harveyi</em> solution containing 10<sup>6</sup> CFU/mL. The results showed that before challenge, synbiotic feed treated shrimp has a growth rate (5.89%), feed conversionrate (1.21), and a high survival rate (80%). After challenge, survival rate (83.33%) of shrimp fed diet supplemented with synbiotic was higher than prebiotic (51.67%) and positive control (31.67%).</p> <p>Keywords: probiotic, prebiotic, synbiotic, <em>Vibrio harveyi</em>,Pacific white shrimp</p>

scholarly journals Physiological and immune response of Litopenaeus vannamei undergoing the acute phase of the necrotizing hepatopancreatitis disease and after being treated with oxytetracycline and FF

2017 ◽  
Vol 44 (3) ◽  
pp. 535-545
Author(s):  
Luis R. Martínez-Córdova ◽  
Teresa Gollas-Galván ◽  
Estefanía Garibay-Valdez ◽  
Rocío Valenzuela-Gutiérrez ◽  
Marcel Martínez Porchas ◽  
...  
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Acute Phase ◽  
Litopenaeus Vannamei ◽  
Positive Control ◽  
Negative Control ◽  
Negative Effect ◽  
Clotting Protein ◽  
Immune Related Genes ◽  
Shock Proteins

The physiological and immune responses of adult shrimp (Litopenaeus vannamei) undergoing the acute phase of the necrotizing hepatopancreatitis (NHP) disease and the efficiency of oxytetracycline (OXI) and florfenicol (FF) to eliminate the pathogen were evaluated. Four shrimp groups were considered: three groups infected with necrotizing hepatopancreatitis bacteria (NHP-B) (two treated with antibiotics and a positive control) and one group non-infected (negative control). Hemolymph concentration of glucose, lactate, acylglycerides, cholesterol, total protein, aminotransferases, superoxide dismutase, and the transcriptional expression of several immune related genes were monitored at the acute phase of the disease, and at 15 and 20 days after administration of antibiotics (daa). Shrimp from the positive control registered a mortality of 100%. NHP-B infection affected the immu-nophysiological response of shrimp; herein, most of the parameters were significantly up regulated in infected shrimp before the use of antibiotics, compared to the negative control. Increased transcriptional levels of clotting protein, lipopolysaccharide and β-1-3-glucan binding protein (LGBP), serine protease, peroxinectin, lysozyme, heat shock proteins (HSP) 60 and 70 were detected in shrimp treated with OXI. At 20 daa NHP-B was still detected in FF-treated shrimp, but not in OXI-treated shrimp. It is hypothesized that despite none of the antibiotics per se eliminated the bacterium, both had a negative effect on its virulence. OXI seems to have a greater effect, allowing shrimp to integrate a better immune response at 15 daa.

Effect of Vitomolt supplements in feed on growth and survival rate of white shrimp (Litopenaeus vannamei) seeds

2020 ◽  
Vol 10 (11) ◽  
pp. 795-798
Author(s):  
Zainuddin . ◽  
Y. Fujaya ◽  
M.I. Djawad ◽  
S. Aslamyah ◽  
K. Nur ◽  
...  
Keyword(s):  
Survival Rate ◽  
Litopenaeus Vannamei ◽  
White Shrimp ◽  
Growth And Survival
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