scholarly journals Effect of Hybrid Rye and Maize Grain Processing on Ruminal and Postruminal Digestibility Parameters

2020 ◽  
Vol 20 (3) ◽  
pp. 1065-1083 ◽  
Author(s):  
Patrycja Rajtar ◽  
Paweł Górka ◽  
Tomasz Schwarz ◽  
Piotr Micek
Keyword(s):  
Dry Matter ◽  
Gas Production ◽  
Lactating Cows ◽  
Grain Processing ◽  
True Digestibility ◽  
Hybrid Rye ◽  
Maize Grain ◽  
Pass Through ◽  
In Sacco

AbstractThe aim of the study was to determine the effect of the method of fragmentation of hybrid rye and maize grain on digestibility parameters for ruminants. Varying degrees of fragmentation – none (whole grains; WG), crushed (CG) or ground to pass through a 4.0 (GG4.0) or 1.5 mm (GG1.5) screen – were estimated by the in sacco nylon bag technique, in vitro true digestibility (IVTD), and the in vitro gas production (GP) technique. WG, CG, GG4.0 and GG1.5 were categorized as none, slight, moderate and extensive fragmentation of the grain kernel, respectively. Three non-lactating cows fitted with ruminal and duodenal cannulas were used to determine the effective rumen degradability (ERD) and intestinal and total tract digestibility (ID and TTD, respectively) of dry matter (DM), crude protein (CP) and starch. IVTD was determined with an ANKOM DaisyII Incubator, and GP and in vitro organic matter digestibility were determined with an ANKOMRF Gas Production System. Dry matter, CP and starch of hybrid rye grain were rapidly degraded in the rumen, and this degradability as well as ID and TTD was only marginally affected by the method of kernel fragmentation; however, among the methods used, the ERD of GG4.0 rye was the lowest. On the other hand, the greater the degree of kernel fragmentation, the higher ERD, ID and TTD were obtained for the DM, CP and starch of maize grain. In summary, rye grain is more susceptible to fermentation in the rumen than maize grain, but the means of grain processing may alter the rate, extent and site of their digestion, particularly for maize grain.

scholarly journals In vitro and in sacco digestibility of almond hulls

2021 ◽  
Vol 159 (7-8) ◽  
pp. 615-621
Author(s):  
K. L. Swanson ◽  
H. M. Bill ◽  
J. Asmus ◽  
J. M. Heguy ◽  
J. G. Fadel ◽  
...  
Keyword(s):  
Dry Matter ◽  
Nutritional Value ◽  
Nutritive Value ◽  
Gas Production ◽  
Metabolizable Energy ◽  
The Other ◽  
Neutral Detergent Fibre ◽  
True Digestibility ◽  
In Sacco

AbstractThe aim of this study was to determine the in vitro digestibility and in sacco disappearance of dry matter (DM) and neutral detergent fibre (NDF) in total almond hulls (TAH), pure almond hulls (PAH) or Debris. The TAH were used because there are no data on the effect of debris (non-hull material) on the nutritional value of almond hulls. Twelve samples of commercial almond hulls were used, with one subsample representing the TAH and the other subsample hand sorted to separate the hulls (PAH) from Debris. Gas production and Ankom Daisy method were used to determine in vitro digestibility, while two rumen-fistulated cows were used to measure in sacco disappearance of PAH and TAH. For in vitro digestibility, both PAH and TAH were more digestible and had greater gas production than Debris. The PAH had greater in vitro true digestibility on a DM basis and NDF digestibility at 48 and 72 h compared with TAH. Nonpareil hulls provided greater metabolizable energy (ME) concentration when compared with other almond varieties, with PAH supplying numerically more ME than TAH for both varieties. For in sacco disappearance, PAH had greater DM and NDF disappearance along with a greater rate of disappearance for NDF compared with TAH. This research demonstrated that Debris is highly indigestible; therefore, hulling, agronomic and harvesting practices should be focused on reducing Debris in commercial almond hulls to improve their nutritive value as a feedstuff for livestock.

scholarly journals Effect of substituting maize grain with cobs treated with a combination of urea and wood ash on sheep performance and rumen parameters

2021 ◽  
Vol 48 (5) ◽  
pp. 343-352
Author(s):  
A. Abdulazeez ◽  
C. M. Tsopito ◽  
O. R. Madibela
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
Average Daily Gain ◽  
Wood Ash ◽  
Gas Production ◽  
Feed Conversion ◽  
Maize Grain ◽  
Maize Cobs

Effect of treating maize cobs with a combination of urea and wood ash (WA) on chemical composition, in vitro gas production, and in sacco degradability were examined in a previous study. The treatments were: (100U) =100% urea +0% wood ash, (75U25WA) = 75% urea +25% wood ash, (50U50WA) = 50% urea +50% wood ash, (25U75WA) =25 % urea + 75% wood ash and (0U0WA) = Untreated maize cobs. It was concluded in the study that, treatment 25U75WA gave better results in terms of all the parameters measured. This study aimed at determining the effect of feeding Twana sheep diets whose grain was substituted with maize cobs treated with 25%U and 75%WA on nutrient intake, digestibility, nitrogen utilization, rumen NH3, pH and performance. Tswana sheep were fed experimental diet whose maize grain was substituted with graded levels of 25U75WA in the dietary ingredients and the treatments were: 100M = 100% maize grain, 66M34C = 66% maize grain plus 34% treated maize cobs, 34M66C = 34% maize grain plus 66% treated maize cobs and 100C = 100% treated maize cobs in a 4×4 Latin Square Design (4 animals were rotated in 4 periods for all the treatments). Treatment 34M66C had crude protein intake (CPI), dry matter intake (DMI), organic matter intake (OMI) and neutral detergent fibre intake (NDFI) of 0.1334, 0.9500, 0.8921 and 0.4800 kg/d respectively (P<0.05) while the crude protein digestibility (CPD), dry matter digestibility (DMD), organic matter digestibility (OMD) and neutral detergentfibre digestibility (NDFD) were 79.57, 68.02, 66.26, and 57.8% respectively (P<0.05). All the treatments had similar (P>0.05) rumen NH3 ranging from 23.55 – 24.00 mg/ml while rumen pH were different (P<0.05) and ranged from 6.117 – 6.937 however treatment 100M had lower pH. Treatment 100M and 34M66C had average daily gain (ADG) of 0.1150 and 0.1417 kg/d, respectively (P<0.05), however, treatment 100M had lower feed conversion ratio (FCR) of 5.217 compared to 7.059 (P<0.05) for 34M66C. It was concluded that farmers should include 34M66C in the diet of Tswana sheep for optimum performance and also reduce the cost of feed.     Effet du traitement des épis de maïs avec une combinaison d'urée et de cendres de bois (CB) sur la composition chimique, la production de gaz in vitro et dans la dégradabilité de SACCO ont été examinées dans une étude précédente. Les traitements étaient: (100u) = 100% urée + 0% de cendre de bois, (75U25CB) = 75% d'urée + 25% de cendres de bois, (50u50wa) = 50% d'urée + 50% de cendre de bois, (25U75CB) = 25% d'urée + 75% de cendres bois et (0U0CB) = épis de maïs non traité. Il a été conclu dans l'étude que, le traitement 25U75CB a donné de meilleurs résultats en termes de tous les paramètres mesurés. Cette étude visait à déterminer l'effet de l'alimentation des régimes de moutons Twana dont le grain a été substitué aux épis de maïs traité avec 25% U et 75% de CB sur l'apport nutritionnel, la digestibilité, l'utilisation de l'azote, le rumen NH3, la performance de la pharmacie. Les moutons de Tswana ont été nourris à une alimentation expérimentale dont le grain de maïs était substitué par des niveaux de grade de 25U75WA dans les ingrédients alimentaires et les traitements étaient les suivants: 100m = 100% de grain de maïs, 66 m34c = 66% de grain de maïs de maïs plus 34% d'épis de maïs traité, 34m66c = 34% de maïs Grain Plus 66% COBS de maïs traité et 100c = 100% cobs de maïs traités dans un design carré latin 4 × 4 (4 animaux ont été tournés en 4 périodes pour tous les traitements). Traitement 34M66C avait une consommation de protéines brutes (CPB), une consommation de matière sèche (CMS), une consommation de matière organique (CMO) et une consommation de fibres de détergents neutres de 0,1334, 0,9500, 0,8921 et 0,4800 kg / j respectivement (p <0,05) tandis que le Digestibilité des protéines brutes (DPB), digestibilité de la matière sèche (DMS), digestibilité de la matière organique (DMO) et digestibilité de fibres de détergent neutres (DFDN) étaient de 79,57, 68,02, 66,26 et 57,8% respectivement (p <0,05). Tous les traitements avaient des analgésiques similaires (p> 0,05) Rumen NH3 allant de 23,55 à 24,00 mg / ml tandis que le pH de rumen était différent (p <0,05) et allait de 6,117 - 6,937Cependant, le traitement 100M avait un pH inférieur. Traitement 100m et 34m66C avaient un gain quotidien moyen (GQM) de 0,1150 et 0,1417 kg / j, respectivement (p <0,05), cependant, le traitement 100M avait un rapport de conversion d'alimentation inférieur (CAI) de 5,217 par rapport à 7,059 (p <0,05) pour 34 m66c. Il a été conclu que les agriculteurs comprennent 34 m66c dans le régime alimentaire des moutons de Tswana pour des performances optimales et réduisent également le coût des aliments pour animaux.

Comparison between reconstituted sheep faeces and rumen fluid inocula and between in vitro and in sacco digestibility methods as predictors of intake and in vivo digestibility

1996 ◽  
Vol 126 (2) ◽  
pp. 235-248 ◽  
Author(s):  
I. V. Nsahlai ◽  
N. N. Umunna
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
Gas Production ◽  
Lag Phase ◽  
Forage Legumes ◽  
Intact Male ◽  
The Relationship ◽  
In Sacco

SUMMARYThis study (conducted in Debre Zeit, Ethiopia in 1993) examined (i) the effect of source of inoculum on in vitro dry matter (DM) digestibility (1VDMD) and gas production (GP) and (ii) the IVDMD, GP and in sacco degradability as predictors of in vivo DM digestibility (DMD) and intake. Six ruminally cannulated male sheep (used in the degradability studies and from which rumen fluid was harvested for the in vitro studies) and six intact sheep (from which faeces for reconstitution was obtained) were given teff straw ad libitum supplemented with 200 g/day of concentrate (1:1 mixture of noug (Guizotia abyssinica) cake and wheat middlings). In determining IVDMD on 26 feeds, pepsin-HC1 digestion was replaced with neutral detergent extraction. Microbial GP was measured on these feeds incubated with rumen fluid or reconstituted faeces inocula at various time periods. The degradability of each feed was determined by the nylon bag technique in three sheep. Eighty-eight intact male Ethiopian Menz type sheep (mean liveweight 256 (S.D. = 1·98) kg) were used in a randomized complete block experiment to determine intake and digestibility.Gas production using faeces inoculum (GP-F) was strongly related to GP using rumen fluid inoculum (GP-R) particularly at 48 h (R2 = 0·85; P <0·001) of incubation. The IVDMD obtained with reconstituted sheep faeces inoculum (IVDMD-F) had a strong positive relationship (R2 = 0·88; P <0·0001) with IVDMD measured using rumen fluid (IVDMD-R). The IVDMD values obtained by centrifugation were positively related to those obtained by filtration for the rumen fluid (R2 = 0·61) and reconstituted faeces (R2 = 0·47) inocula.The relationship between degradability and in vivo DMD was generally poor for roughages but improved with the length of incubation for forage legumes, being strongest at 24 h (R2 = 0·54). The in vivo DMD had the strongest relationship with GP-R at 24 h of incubation for roughages (R2 = 0·64) and legumes (R2 = 0·84). Dry matter degradability was poorly related to DM intake for roughages (R2 <0·10), while these were closely related for legumes particularly at 6 h of incubation (R2 = 0·55). The relationship between GP-R and intake was strongest at 12 h of incubation for roughages (R2 = 0·41) but was generally weak for legumes. The IVDMD accounted for a very low proportion of the variation in intake of roughages and legume forages. The gas production method was the best among the methods tested in predicting voluntary roughage intake, but not the intake of legumes. The nylon bag technique gave the best predictors (solubility, rate constant ‘c’ and Lag phase) of legume intake (R2 = 0·98).The discussion suggests explanations for why the nylon bag technique predicted performance from forage legumes better than from roughages, why the technique was much better as a predictor of intake than of in vivo DMD, and why the gas production technique predicted in vivo DMD and not the intake of legumes. Reconstituted faeces inoculum may replace rumen fluid inoculum in in vitro procedures. The IVDMD method is a less accurate predictor of in vivo DMD than GP and rumen degradability constants. Both feed factors and practices inherent in the methodologies may modify the predictiveness of indirect digestibility methods.

Comparison between in vitro gas production and in situ degradation for evaluating rumen dry matter degradation of maize grain and tropical grasses

2006 ◽  
Vol 34 ◽  
pp. 69-72
Author(s):  
R. M. Mauricio ◽  
T.R. Tomich ◽  
R. A. Filho ◽  
R. B. Reis ◽  
L.C. Gonçalves ◽  
...  
Keyword(s):  
Closed System ◽  
Dry Matter ◽  
Gas Production ◽  
Production Technique ◽  
In Vitro Gas Production ◽  
Dry Matter Degradation ◽  
In Situ Degradation ◽  
Maize Grain

SummaryThe in situ technique (NB) was compared to the in vitro gas production technique (Gas) in terms of ability to estimate the dry matter degradation (DMD) using high soluble substrates (maize grains) and low soluble substrates (four tropical forages). The experiments (in situ and in vitro) were carried out at the same time using the same cow for both techniques and DMD was estimated at 6, 12, 24, 48 & 96 h. The results showed that DMD from Gas were lower than NB DMD and the correlation for the maize group were lower than those of the grass group which indicated that Gas technique, closed system, has potential to analyse highly soluble substrates, probably, overcoming the particle losses effect demonstrated by the in situ technique.

scholarly journals Evaluation of the Nutrient Composition, In Vitro Fermentation Characteristics, and In Situ Degradability of Amaranthus caudatus, Amaranthus cruentus, and Amaranthus hypochondriacus in Cattle

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 18
Author(s):  
Kim Margarette C. Nogoy ◽  
Jia Yu ◽  
Young Gyu Song ◽  
Shida Li ◽  
Jong-Wook Chung ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Gas Production ◽  
Nutrient Composition ◽  
Neutral Detergent Fiber ◽  
Amaranthus Cruentus ◽  
Amaranthus Caudatus ◽  
Amaranthus Hypochondriacus

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.

scholarly journals Preserving Porphyra umbilicalis and Saccharina latissima as Silages for Ruminant Feeding

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1957
Author(s):  
Margarita Novoa-Garrido ◽  
Carlos Navarro Marcos ◽  
María Dolores Carro Travieso ◽  
Eduarda Molina Alcaide ◽  
Mogens Larsen ◽  
...  
Keyword(s):  
Dry Matter ◽  
Nutritional Value ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Saccharina Latissima ◽  
Porphyra Umbilicalis ◽  
In Vitro Gas Production ◽  
Ruminal Degradability ◽  
Kinetics Of

The study analyzed the characteristics, chemical composition, and in vitro gas production kinetics of Porphyra umbilicalis and Saccharina latissima silages. Each seaweed was ensiled in vacuum bags (three bags/silage) following a 2 × 3 factorial design, with two pre-treatments (unwilted or pre-wilted) and three silage types: unwashed seaweed ensiled without additive; seaweed washed and ensiled without additive; and seaweed washed and ensiled with 4 g of formic acid (FAC) per kg seaweed. Silages were kept for 3 months in darkness at 20 °C. Pre-wilting prevented (p < 0.001) effluent formation and reduced (p ≤ 0.038) the production of NH3-N and volatile fatty acids for both seaweeds. Both pre-wilting and washing increased (p < 0.05) the ruminal degradability of P. umbilicalis silages but not of S. latissima silages. The pH of the FAC-treated silages was below 4.0, but ranged from 4.54 to 6.23 in non FAC-treated silages. DL-lactate concentrations were low (≤23.0 g/kg dry matter) and acetate was the predominant fermentation product, indicating a non-lactic fermentation. The estimated ruminal degradability of the P. umbilicalis and S. latissima silages was as average, 59.9 and 86.1% of that for high-quality rye-grass silages, respectively, indicating a medium-low nutritional value of these seaweed silages for ruminants.

EFFECT OF STRUCTURAL AND CHEMICAL FACTORS OF FORAGES ON POTENTIALLY DIGESTIBLE FIBER, INTAKE, AND TRUE DIGESTIBILITY BY RUMINANTS

1988 ◽  
Vol 68 (3) ◽  
pp. 787-799 ◽  
Author(s):  
V. GIRARD ◽  
G. DUPUIS
Keyword(s):  
Cell Wall ◽  
Dry Matter ◽  
Plant Cell Wall ◽  
Neutral Detergent Fiber ◽  
Optimum Number ◽  
Apparent Digestibility ◽  
Cool Season ◽  
Dry Matter Digestibility ◽  
True Digestibility

In view of the large variation found in plant cell wall digestibilities with ruminants, an attempt was made to group 124 feeds into different lignification classes (clusters) on the basis of chemical characteristics. Each feed cluster was described using a structural coefficient [Formula: see text] that related the potentially digestible fiber (PDF, %) to the ratio between lignin and cell wall volume. The optimum number of clusters was determined iteratively by performing a regression of the apparent digestibility of dry matter at maintenance level (DDM1, %) against the PDF and cell soluble (SOL, %) contents of feeds. The [Formula: see text] coefficients varied from 0.05 (grains, N = 13) to 1.85 (corn silage, N = 3) and increased with the maturity of the grasses from 0.88 (legumes, vegetative cool season grasses, N = 26) to 1.33 (mature, cool season grasses, N = 19). Predicted PDF were closely correlated (r > 0.9, P < 0.01) to in vitro cell wall disappearances (IVCWD). Apparently digestible cell wall in four grasses and four legumes increased linearly with 96-h IVCWD and standard error (SE) was similar to the SE of predicted apparent digestible SOL from SOL concentrations. Assuming that similarity between SE could be also observed in larger samples, PDF and SOL were used in summative equations to predict apparent dry matter digestibility. DDM1 discounted for intake (DDM1 – 4, %) was regressed against SOL and PDF concentrations of 87 feeds:[Formula: see text]with ds and df, the true digestibilities of SOL and PDF. Estimates of ds and df were 0.98 and 0.95 for a zero-production (maintenance) level of intake, and 0.91 and 0.79 for an intake level four times maintenance. Since the true digestibility of the PDF component was only 4% – 13% lower than that of the cell soluble component, the concentration of PDF in cell wall was the major determinant in the variation in apparent digestibility of forages. Key words: lignin, neutral detergent fiber, true digestibility, cluster analysis, feeds

The need to complement in vitro gas production measurements with residue determinations from in sacco degradabilities to improve the prediction of voluntary intake of hays

1997 ◽  
Vol 64 (1) ◽  
pp. 71-75 ◽  
Author(s):  
M. Blümmel ◽  
P. Bullerdieck
Keyword(s):  
Volume Ratio ◽  
Gas Production ◽  
Published Data ◽  
Fatty Acid Production ◽  
Residue Determination ◽  
In Vitro Gas Production ◽  
Gas Volume ◽  
In Sacco Degradability ◽  
In Sacco

AbstractThe need to complement in vitro gas production measurements with residue determination is demonstrated by the recalculation and reassessment of published data on in vitro gas production, in sacco degradabilities and voluntary dry matter intake (DMI). The in sacco degradability — gas volume ratio was determined at 24 and 48 h of incubation, termed partitioning factor (PF) and combined with rate and extent parameters of in sacco degradability and in vitro gas production to predict DMI. In vitro gas production and in sacco degradability characteristics (a + b) and c as described by the equation y = a + b(1−ect) explained 0·373 and 0·668 respectively of the variation in DMI of 19 legume and grass hays. The complementation of gas production parameters by the PF24 increased the R2 value to 0·744 with PF24 accounting for 0·407 of the variation in DMI, the rate of gas production (c) for 0·218 and the extent of gas production (a + b) for 0·119 of the variation in DMI. As a single parameter, PF48 showed the highest correlation (R2 = 0·597) with DMI but the combination of PF4S with rate and extent of in sacco or in vitro gas production measurements did not improve the correlation further, probably due to an intercorrelation between rates of fermentation and PF4S. Hays which were degraded at faster rates had higher PF values indicating proportionally higher microbial yield and lower short-chain fatty acid production per unit substrate degraded. Generally, hays with high in sacco degradabilities but proportionally low gas production i.e. hays with high PF values showed higher DMI.

Condensed tannins extracted from Quebracho affect the fermentation profile, nutritional quality and in vitro gas production of pornunça (Manihot spp.) silages

2021 ◽  
pp. 1-10
Author(s):  
D. T. Q. Carvalho ◽  
A. R. F. Lucena ◽  
T. V. C. Nascimento ◽  
L. M. L. Moura ◽  
P. D. R. Marcelino ◽  
...  
Keyword(s):  
Nutritional Quality ◽  
Dry Matter ◽  
Condensed Tannin ◽  
Gas Production ◽  
Rapid Decline ◽  
Randomized Design ◽  
In Vitro Gas Production ◽  
Acid Detergent Fibre ◽  
Fermentation Profile

Abstract The objective was to evaluate the fermentation profile, in vitro gas production and nutritional quality of pornunça (Manihot spp.) silages containing levels of condensed tannin (CT; 0, 4, 8 and 12% on dry matter (DM) basis), at five opening times (0, 3, 7, 14, 28 and 56 days). A completely randomized design in a 4 × 5 factorial arrangement was adopted, with four replications, totalling 80 experimental silos. The pH and NH3-N analyses were performed at all opening times of the silos. The other analyses were performed only with silages opened at 56 days of storage. There was an interaction effect between CT levels and silo opening times for pH and NH3-N. Tannin levels in pornunça silages after 56 days ensiling increased the pH and DM and reduced crude protein (CP) and neutral detergent fibre (NDF). There was a quadratic effect for NH3-N, acetic acid, butyric acid, gas losses, dry matter recovery (DMR), hemicellulose and acid detergent fibre. Inclusion of 4 and 8% CT in pornunça silage promotes a rapid decline in pH, being within the acceptable limit for adequate fermentation at 3 days of ensiling. Silages with 4% CT establish the pH at 28 days of opening the silos, with reduced NH3-N. Silages with 4% CT present higher concentrations of acetic and butyric acids and greater DMR. Inclusion of CT in pornunça silage after 56 days ensiling increases DM and reduces CP and NDF, directly affecting the in vitro degradability and reducing gas production.

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