scholarly journals Diversity in growth and expression pattern of PoHKT1 and PoVHA transporter genes under NaCl stress in Portulaca oleracea taxa

Genetika ◽  
2016 ◽  
Vol 48 (1) ◽  
pp. 233-248
Author(s):  
R. El-Bakatoushi ◽  
A. Elframawy

Plant growth and the expression of two transporter genes; PoHKT1 and PoVHA transcripts in root and shoot tissues were studied under salt stress of three Portulaca oleracea s.l. taxa. The study showed no significant differences in ratios between root lengths in saline and non-saline treatments of the three taxa, which was correlated with a clear down-regulation of the PoHKT1 transcripts in the root after 150mM NaCl. All measured growth parameters except root length increased in P. oleraceae, decreased in P. granulatostellulata and remain unchanged after 100mM NaCl in P. nitida compared to control under saline conditions. The result was consistent with the type of taxon which had significant effect on the shoot length, number of leaves and dry weight (P< 0.05). All measured growth parameters except root length showed a significant negative correlation with the shoot fold change of PoHKT1 transcripts (r = -0.607, -0.693 and -0.657 respectively). The regulation of PoVHA in root and shoot tissues in the three taxa are significantly different. Under salt stress, both decreased uptake of Na+ into the cytosol by decreasing the expression of PoHKT1 and increased vascular compartmentalization ability of Na+ by inducing the expression of PoVHA seem to work more efficiently in P. oleraceae and P. nitida than in P. granulato-stellulata.

Author(s):  
Saiteja Atluri ◽  
Deepshikha Thakur ◽  
Dinesh Bukke ◽  
Naleeni Ramawat

The field experiment was conducted in Organic farm of Amity university Noida, Uttar Pradesh, during the kharif season, to evaluate the sole and conjoint effect of biofertilizers and biochar on the yield, growth and productivity of Soybean (Glycine max). The five treatments viz. T1 (Control), T2 (Biochar), T3 (Biochar + Rhizobium), T4 (Biochar + Azospirillum) and T5 (Biochar + Rhizobium + Azospirillum) were used. Plant growth parameters like shoot length, root length, number of leaves, pod length, pod girth, plant height, crop yield and soil parameters were observed for the effect of various treatments. Among the all treatments the treatment T3 (Biochar with Rhizobium) maximum shoot length (52.30), root length (17.30) followed by T4 (Biochar + Azospirillum) and plant productive are maximum mean of number of pods was recorded in T3 (Biochar + Rhizobium) i.e. 32.6, whereas minimum (30.1) was recorded in T1(control) followed by T4(Biochar + Azospirillum) i.e.32.41, T5 (Biochar + Rhizobium + Azospirillum) i.e. 31.05 and T2 (Biochar) i.e.31.31   has shown significant effect on plant growth characters and plant yield. These results indicate that the   conjoint use of biochar and Rhizobium have potential to enhance the crop performance and simultaneously improves the soil properties for sustainable farming without reliance on synthetic agrochemicals.


Author(s):  
Aynur Sadak ◽  
Abdulrahman Smail İbrahim ◽  
Suat Şensoy

The study was carried out as a pot experiment in controlled conditions in order to reveal the effect of endophyte bacteria (EB) applications on the development of pepper seedlings grown under different drought stress. Mostar F1 was used as pepper variety. Applied endophyte bacterial isolates were Ochrobactrum sp. (CB36/1) and Bacillus sp. (CA41/1). The EB application was applied twice (first application 10 ml- second application 15 ml) at a density of 109 cfu/ml. All pots were irrigated regularly for 52 days after seed sowing. While irrigation was continued at 2-day intervals in control (B0) applications, four and eight days drougth stresses were applied in the second (B1) and last (B2) application. As plant growth parameters, stem diameter, shoot length and root length, shoot fresh and dry weights, leaf number, root fresh and root dry weight were examined. It has been determined that the effects of B1 and B2 stress applications on plant growth were generally negative. It has been determined that endophyte bacteria applications have relatively positive effects against the negativity caused by different drought stresses.


2020 ◽  
Vol 13 (2) ◽  
pp. 83-92 ◽  
Author(s):  
A. Adam

SummaryEnhancement of the resistance level in plants by rhizobacteria has been proven in several pathosystems. This study investigated the ability of four rhizobacteria strains (Pseudomonas putida BTP1 and Bacillus subtilis Bs2500, Bs2504 and Bs2508) to promote the growth in three barley genotypes and protect them against Cochliobolus sativus. Our results demonstrated that all tested rhizobacteria strains had a protective effect on barley genotypes Arabi Abiad, Banteng and WI2291. However, P. putida BTP1 and B. subtilis Bs2508 strains were the most effective as they reduced disease incidence by 53 and 38% (mean effect), respectively. On the other hand, there were significant differences among the rhizobacteria-treated genotypes on plant growth parameters, such as wet weight, dry weight, plant height and number of leaves. Pseudomonas putida BTP1 strain was the most effective as it significantly increased plant growth by 15-32%. In addition, the susceptible genotypes Arabi Abiad and WI2291 were the most responsive to rhizobacteria. This means that these genotypes have a high potential for increase of their resistance against the pathogen and enhancement of plant growth after the application of rhizobacteria. Consequently, barley seed treatment with the tested rhizobacteria could be considered as an effective biocontrol method against C. sativus.


2016 ◽  
Vol 2 (1) ◽  
pp. 74-81 ◽  
Author(s):  
Sakil Mahmud ◽  
Shayla Sharmin ◽  
Bishan Lal Das Chowdhury ◽  
Mohammad Anowar Hossain ◽  
Muhammad Javidul Haque Bhuiyan

To explore the possibility of using methyl jasmonate (MeJA) for alleviation of salt stress, the present study was conducted where six rice varieties (BRRI dhan31, BRRI dhan46, Gota, Kajalsail, Pokkali and Pengek) were grown in non saline (0 dSm-1), saline (12 dSm-1), saline (12 dSm-1) + 10 ?M MeJA and saline (12 dSm-1) + 20 ?M MeJA conditions at germination stage (till the 9 days). MeJA was applied by imbibing seeds in it for 24 hours. To evaluate the effect of MeJA on saline stressed plant at germination stage, change in growth parameters namely germination percentage, shoot and root length, fresh and dry wt. of shoot, fresh and dry wt. of root and biochemical component- activity of alpha-amylase were monitored. Salinity had a minimum effect on final germination percentage (FGP) of rice varieties, but delayed in attaining it. MeJA had least positive effect. Salinity significantly reduced the shoot and root length, fresh and dry weight of all the varieties. MeJA had more decreasing effect in susceptible varieties while increasing effect in moderately tolerant and tolerant. Activity of alpha-amylase in germinated seed varied from 0.051 mg to 0.111 mg mal./mg tissue. At 12dSm-1 salinity, susceptible and moderately tolerant varieties showed significant reduction but tolerant showed significant increase. MeJA intensified the negative effect further in susceptible varieties. In tolerant and moderately tolerant varieties, increment in activity took place and 10 ?M had a better effect over 20 ?M MeJA.Asian J. Med. Biol. Res. March 2016, 2(1): 74-81


2021 ◽  
Vol 2 ◽  
pp. 234-239
Author(s):  
Nada Kholifah ◽  
Ardiana Kartika B ◽  
Teguh Pribadi

PGPR (Plant Growth Promoting Rhizobacteria) is a substance that helps plant growth with the help of rhizosphere microorganisms. PGPR propagation can be done with liquid media. This PGPR propagation needs to be done because this substance has many benefits for agricultural cultivation. The application of PGPR to the test plant, namely the pakcoy plant, proved that there was an effect of giving PGPR to the plant. Observations on the test plants were carried out by observing several observation variables such as plant height, root length, number of leaves, wet weight and dry weight. The results of these observations showed that the effect on the test plants was seen in the variables of root length, wet weight, and dry weight. Meanwhile, the variable plant height and number of leaves did not show a visible difference. 


2016 ◽  
Vol 8 (6) ◽  
pp. 71
Author(s):  
Geetha Karuppasamy ◽  
Michael Antony D'Couto ◽  
Anant Achary

<p>The fruits and vegetables lost due to spoilage in the market can be converted to nutrient rich organic biofertilizer, vermicompost and worm tea. In this study, non-edible vegetables from market [French beans (S1), Lady’s fingers (S2) and Brinjal (S3)] were used for production of vermicompost and worm tea using <em>Eisenia fetida</em> for environmental friendly management and recycling, as value added product for crop production. Vermicomposting was carried out in four bins. Three bins (S1, S2 &amp; S3) consisted of individual pre-digested vegetables and the fourth bin (S4) was set up with a mixture of all three pre-digested vegetables in equal proportions. Physico-chemical properties and essential nutrients like pH, temperature, moisture content, total organic carbon (C), total nitrogen (N), available phosphorus (P) and exchangeable potassium (K) of the pre-digested vegetables and/or compost were analysed at regular time intervals. There was around 6-10 fold increase in earthworm count at the end of vermicomposting. During vermicomposting, the N and P content of the compost showed 2 to 4 fold increase whereas 10 to 15 fold increase in K content was noticed. Low C:N ratio (4 to 9) was obtained for vermicompost VS4. The worm tea (vermi wash) and vermicompost collected were used for plant growth studies on <em>Vigna radiata</em> (Green gram). Growth parameters like germination percentage, vigour index, germination index (GI), shoot length (cm), root length (cm) and leaf length (cm) were studied. There was noticeable improvement in germination % (1.7 fold), vigour index (2.7 fold) and germination index. A 20 fold increase in shoot length was also seen in test plants when compared to control (2 fold). Statistical analysis of various growth parameters like root length and plant height indicated that vermicompost made with waste brinjal has a significant response with p ≤ 0.05. Based on the results obtained, waste brinjal, which is abundantly available locally can be economically converted to organic biofertilizers and used for soil and crop improvement. Through this study, a cost effective and environment friendly method for efficient utilization of market waste vegetables has been proposed for promoting plant growth and development.</p>


Author(s):  
Anita Mann ◽  
Gurpreet Kaur ◽  
Ashwani Kumar ◽  
Satish Kumar Sanwal ◽  
Jogendra Singh ◽  
...  

Screening of chickpea lines for salt tolerance through seed germination and early seedling growth is crucial for their evaluation. Seeds of 30 chickpea genotypes were germinated on a sand bed irrigated with saline (3, 6, 9, 12 dS/m) and control solutions upto 30 days. At the early seedling stage (25-30 days), germination percentage, chlorophyll content, proline, root length, shoot length and seedling dry weight were found to be affected due to salinity. Salt tolerance index (STI) for plant biomass maintained a significant correlation with chlorophyll, proline, shoot length, and root length, which indicated that these parameters could be used as selection criteria for screening chickpea genotypes against salt stress. Significant differences in shoot length, root length, and seedling dry weight in 30-day-old seedlings were observed among selected chickpea genotypes as well. From the overall observation of germination characterstics and early seedling growth, it is concluded that the chickpea genotypes, HC-1, HC-5, ICC 867, ICC 5003, H-10-41 showed better salt tolerance as compared to the available salt tolerant check variety.


2010 ◽  
Vol 56 (No. 4) ◽  
pp. 194-199 ◽  
Author(s):  
M. Kabir ◽  
M.Z. Iqbal ◽  
M. Shafiq ◽  
Z.R. Farooqi

The effects of lead on root, shoot and seedling length, leaf area, number of leaves, plant circumference, seedling dry weight, root/shoot and leaf area ratios of <I>Thespesia populnea</I> L. were determined in greenhouse under natural environmental conditions with and without phytotoxic metal ions at 5, 10, 15, 20, and 25 µmol/l. Lead treatments have a strong influence on the growth and development of <I>T</I>. <I>populnea</I> by reducing significantly (<I>P</I> &lt; 0.05) all the above parameters. Lead treatment at 5–25 µmol/l produced significant (<I>P</I> &lt; 0.05) effects on seedling and root length, plant circumference and seedling dry weight of <I>T</I>.<I> populnea,</I> while lead treatment at 10–25 µmol/l produced significant (<I>P</I> &lt; 0.05) effects on shoot length, number of leaves and leaf area as compared to control. Tolerance in <I>T</I>.<I> populnea</I> seedling at 25 µmol/l of lead treatment was lowest as compared to all other treatments.


Author(s):  
S Naseem ◽  
Q Ali ◽  
A Malik

Maize is an important cereal crop lies at third after wheat and rice in Pakistan. It is very sensitive to salt, heat, drought, cold and heavy metal stresses. To evaluate the effects of salt (NaCl) on maize plant we have conducted a research experiment in the Green House of Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore. We select hybrid P1429, P5971 and P6103 of Maize to access the effect of different concentrations of NaCl stress included control, 0.25Molar NaCl, 0.5Molar NaCl, 0.75Molar NaCl, and 1Molar NaCl applications. The pots were filled with pure sand and seeds of each hybrid were sown in each pot and were let to germinate. After 7 days of germination the data was collected for leaf length, root length, shoot length and root/shoot length ratio. The pots were then given the treatment and data of above mentioned traits was recorded, the application of treatments and data recording were repeated 4 times. The data recorded (4 times each after one week) was subjected to pooled analysis of variance to find significant differences among hybrids and treatments. The result of our study showed that there were significant correlation among root length, shoot length and leaf length of seedlings, from average performance of hybrid P6103 was better as compared with other two hybrids under higher salt stress conditions. It was suggested from our study that the hybrid P6103 may be used as salt tolerance hybrids for improving grain and fodder yield of maize under salt stress condition.


2021 ◽  
Vol 23 (2) ◽  
pp. 51-58
Author(s):  
MAT Sohel ◽  
MAE Hossain ◽  
HP Roy ◽  
SM Reza ◽  
FH Shanta ◽  
...  

The experiment was carried out at the research field of Agronomy and Farming Systems Division, Bangladesh Sugarcrop Research Institute (BSRI), Ishurdi, Pabna during 2012-2013 to determine the most suitable spacing for sugarbeet cultivation in Bangladesh. The experiment was conducted with nine spacing viz. 50 cm × 20 cm, 60 cm × 20 cm, 70 cm × 20 cm, 50 cm × 25 cm, 60 cm × 25 cm, 70 cm × 25 cm, 50 cm × 30 cm, 60 cm × 30 cm and 70 cm × 30 cm in a randomized complete block design with three replications. The effects of spacing on sugarbeet plantation were observed on growth and growth contributing components (germination percentage, number of leaves plant-1, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, shoot dry weight, crop growth rate) of sugarbeet. The highest germination percentage (95.67%), number of leaves plant-1 (34.33) at 30 DAS, shoot length (54.07 cm) at 120 DAS, root fresh weight (969.47 g plant-1) at 150 DAS, shoot fresh weight (752.47 g plant-1) at 120 DAS and other growth contributing parameters were obtained with the spacing 70 cm × 30 cm. However, the maximum root length (38.97 cm) was obtained with 50 cm × 20 cm spacing. It was concluded that the wider spacing promoted the growth of individual beet, though the optimum spacing for maximum root growth of sugarbeet was 50 cm × 20 cm. Bangladesh Agron. J. 2020, 23(2): 51-58


Sign in / Sign up

Export Citation Format

Share Document