scholarly journals DETERMINATION OF VITAMIN C IN SEVERAL VARIETIES OF MELON FRUITS BY TITRATION METHOD

2017 ◽  
Vol 17 (2) ◽  
pp. 118
Author(s):  
Nerdy .
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Immune Function ◽  
Water Soluble ◽  
Analytical Chemist ◽  
Titration Method ◽  
Science Publisher ◽  
Official Methods Of Analysis ◽  
Melon Fruit

Abstract. Vitamin C (ascorbic acid) is micronutrient that is water soluble and indispensable for the body's metabolism, also plays a role in immune function. Vitamin C are easy to be absorbed actively. One of the fruits that contain vitamin C is melon fruit. Many varieties of melon fruit affect the vitamin C levels. The levels of vitamin C can be determined by 2,6-dichloroindophenol titration method using 2,6-dichloroindophenol solution. The advantages of the 2,6-dichloroindophenol titration method in the determination of vitamin C levels is reductor substances other than vitamin C are not oxidized by 2,6-dichloroindophenol. The examination begins with the determination of equivalence of 2,6-dichloroindophenol solution to vitamin C and followed by determination of vitamin C levels in several varieties of melon fruit. Equivalence of 2,6-dichloroindophenol solution to vitamin C was obtained 0,1347 mg vitamin C per 1 mL 2,6-dichloroindophenol solution. Levels of vitamin C in various varieties of melon fruit obtained that the highest levels was in Golden Melon variety (9,209 mg vitamin C per 100 g of Golden Melon) while the lowest levels was in Langkawi Melon variety (0,335 mg of vitamin C per 100 g of Langkawi Melon). It can be concluded that vitamin C levels contained in melon fruit vary and depend on melon fruit varieties.Keywords: Vitamin C, Ascorbic Acid, Melon Fruits, Varieties, Titration MethodREFERENCE S. Almatsier, 2001, Prinsip Dasar Ilmu Gizi, Gramedia Pustaka Utama, Jakarta, 152-153, 185-186.F. Prajnanta, 2003, Melon: Pemeliharaan secara Intensif dan Kiat Sukses Beragribisnis. Penebar Swadaya, Jakarta, 3, 30.R. Rukmana, 2007, Melon Hibrida, Kanisius, Yogyakarta, 11, 13-15, 16.B. Samadi, 2007, Melon: Usaha Tani dan Penanganan Pascapanen, Kanisius, Yogyakarta, 19, 23, 25-28, 31.P.M. Wijoyo, 2009, Panduan Praktis Budi Daya Melon, Bee Media Indonesia, Jakarta, 14-17, 55.I.G. Gandjar, dan A. Rohman, 2007, Kimia Farmasi Analisis, Pustaka Pelajar, Yogyakarta, 249.N. Andarwulan, dan S. Koswara, 1992, Kimia Vitamin, Rajawali Press, Bogor, 14-20, 32-35, 235.Direktorat Jenderal Pengawasan Obat dan Makanan Departemen Kesehatan Republik Indonesia, 1995, Farmakope Indonesia, Edisi Ke-4, Departemen Kesehatan Republik Indonesia, Jakarta, 1215-1216.W. Horwitz, 2002, Official Methods of Analysis of Association Offical Analytical Chemist Internasional, Edisi Ke-42, Association Offical Analytical Chemist International Suite, Maryland, 16-17.10. J.N. Counsell, and D.H. Hornig, 1981, Vitamin C, Applied Science Publisher, London, 123-124.11. R.M. Astrid, 2016, Cara Cerdas Berkebun Emas Dengan Menanam Melon, Villam Media, Depok, 67. 

scholarly journals Determination of Ascorbic Acid in Oman Citrus Fruits

2007 ◽  
Vol 3 ◽  
pp. 7
Author(s):  
KS.M.Z. Al-Kindi ◽  
A.O. Abdelnour ◽  
Rahma S. Al-Siyabi
Keyword(s):  
Ascorbic Acid ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin C ◽  
High Performance ◽  
Oxidizing Agent ◽  
Citrus Fruits ◽  
Titration Method ◽  
Different Temperatures

Omani citrus fruits were analyzed for their Vitamin C (ascorbic and AA) content by a titration method involving  N-bromosuccinimide (NBS) as an oxidizing  agent and by high performance liquid  chromatography. Vitamin C content was also determined after storage at different temperatures. Vitamin C contents of the fruits were found to lie within  the values reported in the literature.

scholarly journals IDENTIFICATION OF ASCORBIC ACID CONTENT IN Carica papaya L. USING IODIMETRY AND UV-VIS SPECTROPHOTOMETRY

2019 ◽  
Vol 1 (2) ◽  
pp. 58-64
Author(s):  
Nosy Maria Riscahyani ◽  
Evy Ratnasari Ekawati ◽  
Khoirul Ngibad
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Carica Papaya ◽  
Ascorbic Acid Content ◽  
Ammonium Molybdate ◽  
Skin Rejuvenation ◽  
Titration Method ◽  
Body Tissues ◽  
Vegetables And Fruits

Ascorbic acid mostly comes from vegetables and fruits, especially fresh fruits. Vitamin C is a vitamin that can be formed by several types of plants. One of them is papaya which has various contents including vitamin C that can increase endurance, help skin rejuvenation and repair body tissues. The purpose of this study was to determine the levels of vitamin C contained in papaya using iodimetry and UV-Vis spectrophotometry. The sample used in this study was papaya fruit. Determination of vitamin C levels in papaya samples using the titration method bas done by adding sample filtrate with starch indicator then titrated with titrant I2 until the endpoint color of blue titration is formed. Other hands, the determination of vitamin C levels in papaya samples using the UV-Vis spectrophotometry method bas conducted by making an ascorbic acid calibration curve then the filtrated sample was added with H2SO4 5% and ammonium molybdate reagent, then the absorbance of the sample was measured at 494 nm wavelength. The results of the determination of vitamin C levels using the iodimetry was 0.0147% and the results of the determination of vitamin C levels using the UV-Vis spectrophotometry method was 0.1313%. In conclusion, vitamin C levels analyzed by using UV-Vis spectrophotometry methods were greater than vitamin C levels analyzed by using the iodimetry method.

scholarly journals Synthesis of Fluorescent Carbon Dots and Their Application in Ascorbic Acid Detection

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1246
Author(s):  
Tengfei Wang ◽  
Hui Luo ◽  
Xu Jing ◽  
Jiali Yang ◽  
Meijun Huo ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Fluorescence Intensity ◽  
Carbon Dots ◽  
Ph Dependence ◽  
Scale Up ◽  
Water Soluble ◽  
Jujube Fruit ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Water-soluble fluorescent carbon dots (CDs) were synthesized by a hydrothermal method using citric acid as the carbon source and ethylenediamine as the nitrogen source. The repeated and scale-up synthetic experiments were carried out to explore the feasibility of macroscopic preparation of CDs. The CDs/Fe3+ composite was prepared by the interaction of the CDs solution and Fe3+ solution. The optical properties, pH dependence and stability behavior of CDs or the CDs/Fe3+ composite were studied by ultraviolet spectroscopy and fluorescence spectroscopy. Following the principles of fluorescence quenching after the addition of Fe3+ and then the fluorescence recovery after the addition of asorbic acid, the fluorescence intensity of the carbon dots was measured at λex = 360 nm, λem = 460 nm. The content of ascorbic acid was calculated by quantitative analysis of the changing fluorescence intensity. The CDs/Fe3+ composite was applied to the determination of different active molecules, and it was found that the composite had specific recognition of ascorbic acid and showed an excellent linear relationship in 5.0–350.0 μmol·L−1. Moreover, the detection limit was 3.11 μmol·L−1. Satisfactory results were achieved when the method was applied to the ascorbic acid determination in jujube fruit. The fluorescent carbon dots composites prepared in this study may have broad application prospects in a rapid, sensitive and trace determination of ascorbic acid content during food processing.

scholarly journals Spectrophotometric determination of ascorbic acid in grapes with the Prussian Blue reaction

2012 ◽  
Vol 23 (2) ◽  
pp. 174-179 ◽  
Author(s):  
Nicoleta Matei ◽  
Simona Dobrinas ◽  
Gabriel Lucian Radu
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Vitis Vinifera ◽  
Prussian Blue ◽  
Spectrophotometric Determination ◽  
Pinot Noir ◽  
Cabernet Sauvignon ◽  
Red Grapes ◽  
Blue Reaction

AbstractThe objective of the present work was to adapt the Prussian Blue reaction for the determination of ascorbic acid. The procedure was successfully applied for the determination of ascorbic acid in red and white grapes (Vitis vinifera L.) just previous ingathering. In the present work was used the red and white grapes from Murfatlar vineyard: Mamaia, Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, Sauvignon, Muscat Ottonel and Riesling Italian. The results were situated in the range of 0.67 - 1.79 mg vitamin C/100g product for red grapes and respectively 0.50 - 1.49 mg vitamin C/100g for white grapes.

scholarly journals Determination of Ascorbic Acid Concentration of Some Commercial Fruits Juices Sold in Ugbokolo Benue State, Nigeria

2017 ◽  
Vol 3 (1) ◽  
pp. 19-22
Author(s):  
Fabian Audu Ugbe ◽  
Victoria Abiola Ikudayisi ◽  
Oluwagbemiga Tayo Amusan
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Dietary Intake ◽  
Pregnant Women ◽  
Acid Concentration ◽  
Ascorbic Acid Concentration ◽  
Titrimetric Method

This research was aimed at investigating the concentration of Ascorbic Acid (Vitamin C) in some commercial fruits juices: ‘5’ Alive, Hollandia yoghurt, Nutri milk, Ribena, Lacasera, Happy hour, Viju milk, Chi exotic and Chi vita sold in Ugbokolo, Benue State, Nigeria by titrimetric method using 2,6-dichlorophenolindophenol (DCPIP) as titrant. The ascorbic acid (AA) content in milligram per 100ml of the various juices investigated is in the order: ‘5’ Alive (11.25) > Ribena (5.47) > nutri milk (5.31) > Happy hour (4.38) > Hollandia yoghurt (1.25) > Lacasera (0.47) ≈ Chi exotic (0.47) >Viju milk (0.31) > Chi vita (0.16). It was observed that half a litre of ‘5’ Alive (56.25 mg AA) a day is suitable for adults and pregnant women, a liter each of Ribena  (54.70 mg AA) and Nutri milk (53.10 mg AA) per day for adults, while a litre of Happy hour (43.80 mg AA) per day is suitable for adolescents. It can be concluded that commercial fruits juices when taken in large amount will add to the daily human dietary intake of the vitamin.

scholarly journals Iodometric Quantitative Analysis Method of Ascorbic Acid in Tablets

2019 ◽  
Vol 70 (10) ◽  
pp. 3555-3560
Author(s):  
Costinela Valerica Georgescu ◽  
Cristian Catalin Gavat ◽  
Doina Carina Voinescu
Keyword(s):  
Ascorbic Acid ◽  
Quantitative Analysis ◽  
Standard Deviation ◽  
Statistical Significance ◽  
The Body ◽  
Water Soluble ◽  
International Standards ◽  
Titration Method ◽  
Normal Limits ◽  
Relative Standard

Ascorbic acid is a water-soluble vitamin provided with strong antioxidant action, that fulfills an important immune protective role of the body against infections and prevents various cancers appearance. The main goal of this study was to exactly quantify pure ascorbic acid in tablets of two pharmaceuticals. Proposed objective consisted in improvement and application of a iodometric titration method in ascorbic acid quantitative analysis. Ascorbic acid content per tablet in both studied pharmaceuticals was 173.84 mg, very close to official stated amount of active substance (180 mg). Allowed percentage deviation from declared content of pure ascorbic acid was only 3.42 %, below maximum value of � 5 % imposed by Romanian Pharmacopoeia 10-th Edition, according to European and International standards. Statistical analysis confirmed experimental obtained results and revealed low Standard Error value SE = 0.214476, which has fallen within normal limits. Confidence Level value (95.0 %) = 0.551328 and Standard Deviation SD = 0.525357. were within normal range of values. Relative Standard Deviation (Coefficient of variation or homogeneity) RSD = 26.268% was found below maximum range of accepted values (30-35%). P value = 7.44. 10-6 was located within normal limits, P [ 0.001, so the experimental obtained results has shown highest statistical significance. Thus, studied titration method can be successfully used in quantitative analysis of ascorbic acid from different samples.

INFLUENCE OF FOLIAR APPLICATION OF NOVATEK SOLUB K-MAX FERTILIZER ON THE BIOCHEMICAL COMPOSITION AND COMMERCIAL AND CONSUMER QUALITIES OF APPLE FRUITS

2021 ◽  
Vol 64 ◽  
pp. 54-60
Author(s):  
D. R.  Useynov ◽  
E. F. Chelebiev ◽  
О. A. Denisova
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Biochemical Composition ◽  
Foliar Application ◽  
Mineral Fertilizer ◽  
Dry Weight ◽  
Water Soluble ◽  
Apple Fruits

The article presents data on the study of the effect of foliar water-soluble mineral fertilizer NOVATEK Solub K-Max 10-5-30 on the biochemical composition and commercial quality of apple fruits. A rise in vitamin C from the introduction of NOVATEK at a rate of 2.5 kg/ha indicates the effectiveness of this drug for optimizing the content of ascorbic acid in fruits. An increase in the content of monosaccharides and a decrease in disaccharides were found for all the studied rates of consumption of agrochemicals. The use of mineral fertilizer “Novatek Solub K-Max” contributed to increase the dry weight of fruits. The application of this mineral fertilizer is recommended for inclusion in the industry regulations for Apple cultivation as one of the elements of the technology. 

scholarly journals Water- and Fat-Soluble Antioxidants in Human Seminal Plasma and Serum of Fertile Males

Antioxidants ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 96 ◽  
Author(s):  
Giacomo Lazzarino ◽  
Ilaria Listorti ◽  
Gabriele Bilotta ◽  
Talia Capozzolo ◽  
Angela Amorini ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Vitamin E ◽  
Vitamin C ◽  
Seminal Plasma ◽  
Sperm Count ◽  
Venous Blood ◽  
Water Soluble ◽  
Human Seminal Plasma ◽  
Serum Ratio

Reactive oxygen species (ROS) are physiologically involved in functions like sperm maturation, capacitation and acrosome reaction, but their excess is involved in male infertility. Antioxidants in seminal plasma (SP) are an important factor balancing physiologic and harmful ROS activities. In this study, we determined and compared the full profiles of the water- and fat-soluble antioxidants in SP and serum of 15 healthy fertile subjects (ranging between the ages of 35 and 42 years). Ejaculates were obtained after 2–5 days of sexual abstinence. After liquefaction and withdrawal of an aliquot for the sperm count, samples were centrifuged to obtain SP. Thirty min after semen donation, a venous blood sample was collected from each subject. Donors with lower SP concentrations of ascorbic acid (n = 5) or α-tocopherol (n = 5) received a 4 week oral administration of either vitamin C (100 mg/day) or vitamin E (30 mg/day). They were then re-assayed to determine the SP and serum levels of ascorbic acid and α-tocopherol. SP and serum samples were properly processed and analyzed by HPLC methods suitable to determine water (ascorbic acid, glutathione (GSH) and uric acid) and fat-soluble (all-trans-retinoic acid, all-trans-retinol, α-tocopherol, carotenoids and coenzyme Q10) antioxidants. Data demonstrate that only ascorbic acid is higher in SP than in serum (SP/serum ratio = 4.97 ± 0.88). The other water-soluble antioxidants are equally distributed in the two fluids (GSH SP/serum ratio = 1.14 ± 0.34; uric acid SP/serum ratio = 0.82 ± 0.12). All fat-soluble antioxidants are about 10 times less concentrated in SP than in serum. In donors treated with vitamin C or vitamin E, ascorbic acid and α-tocopherol significantly increased in both fluids. However, the SP/serum ratio of ascorbic acid was 4.15 ± 0.45 before and 3.27 ± 0.39 after treatment, whilst those of α-tocopherol were 0.11 ± 0.03 before and 0.10 ± 0.02 after treatment. The results of this study, by showing the peculiar composition in water- and fat-soluble antioxidants SP, indicate that it is likely that still-unknown mechanisms allow ascorbic acid accumulation in SP against a concentration gradient. SP mainly relies its defenses on water- rather than fat-soluble antioxidants and on the mechanisms ensuring their transfer from serum.

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