scholarly journals Perubahan Morfologi dan Pertumbuhan Handeuleum (Graptophyllum pictum L. Griff) akibat Iradiasi Sinar Gamma

2016 ◽  
Vol 43 (3) ◽  
pp. 235
Author(s):  
Arrin Rosmala ◽  
Nurul Khumaida ◽  
Dewi Sukma
Keyword(s):  
Medicinal Plants ◽  
Gamma Rays ◽  
Irradiation Dose ◽  
Leaf Morphology ◽  
Color Index ◽  
Gamma Ray ◽  
Morphological Changes ◽  
Morphological Alteration ◽  
Green Color ◽  
Gamma Ray Irradiation

<p>ABSTRACT</p><p>Handeuleum (Graptophyllum pictum L.Griff) is a medicinal plant widely used as a traditional medicine due to its benefecial content. Therefore, it should be developed as one of the leading Indonesian medicinal plants. The purpose of this research was to study the effect of gamma ray irradiation on morphological alteration and growth of Handeuleum accession from Bogor. The doses of gamma rays were 0, 15, 30, 45, 60, 75, 90, and 105 Gy. The results of the research showed that gamma-ray irradiation induced morphological changes and influences the growth of Handeuleum. Irradiation dose at rate of 105 Gy produced new leaf morphology in Handeuleum, namely cordate. Doses of 60, 75, 90, and 105 Gy produce stunted plants with stiff and yellowish-green leaves. The dose of 45 Gy produced relative green color index which was higher than the control plants.</p><p>Keywords: handeuleum, gamma irradiations</p>

scholarly journals Induksi Mutasi Fisik dengan Iradiasi Sinar Gamma pada Kunyit (Curcuma domestica Val.)

2015 ◽  
Vol 5 (2) ◽  
pp. 84
Author(s):  
Yahidah Rosyidah Anshori ◽  
Syarifah Iis Aisyah ◽  
Latifah K Darusman
Keyword(s):  
Gamma Ray ◽  
Morphological Changes ◽  
Stunted Growth ◽  
Dose Rates ◽  
Yellow Color ◽  
Vegetative Traits ◽  
Gamma Ray Irradiation ◽  
Leaf Deformation ◽  
Rheumatic Heart

<p>ABSTRACT</p><p>Turmeric  is  a  spice plant  and  potential  as a major  ingredient  of  functional food. Turmeric contains curcumin, an active compound which gives the yellow color from its rhizomes that provides health benefits. Curcumin is an antioxidant and acts as an anti-cholesterol as well as a medicine for tumors, cancer, hypertension, hyperglycemia, and rheumatic heart disease. Limited supply of simplicialevel of curcumin based on market standards and the low genetic variability of turmeric as a source for conventional breeding makes this research valuable to be  conducted.  The purposes of this study were  to  obtain  LD50 dose  and  turmeric  crop  yield  variability  due  to  the  changes  in physical mutation  induced  by  gamma-ray  irradiation.  An  acute  single iradiation  was  given  to  using  the universal panoramic irradiator with 11 different dose rates,  The plants  then were cultivated in vivo. The  growth observation  on  turmeric  was  observed  on  vegetative  traits  qualitatively and quantitatively. In this study, the LD50 dose of turmeric was 47.26 Gy. The plant’s vegetative growth tends  to  decelerate  with  the  increase  of irradiation  doses.  The  high  variabillity  growth  for   leaf number  occured  on 50  Gy  of  dose.  Morphological  changes  occured  in  the  form  of  pseudo-stem shape  due  to  irradiation  doses  of  50  and  60  Gy.  Most  leaf  surface discoloration  and  leaf deformation occured at 50 and 70 Gy, and stunted growth occured at 60 and 70 Gy.</p><p>Keywords: curcumin, iradiation, LD50, turmeric</p><p> </p><p>ABSTRAK</p><p>Kunyit merupakan tanaman rempah yang potensial sebagai bahan utama pangan fungsional. Rimpang kunyit mengandung senyawa aktif utama yaitu kurkumin yang memberikan warna kuning pada  rimpang  juga  memberikan manfaat  untuk  kesehatan.  Kurkumin  bersifat  antioksidan  dan berperan sebagai  antikolesterol,  obat  tumor,  kanker,  obat  hipertensi,  hiperglikemia, penyakit  hati serta  rematik.  Keterbatasan  penyediaan  simplisia  yang mempunyai  kandungan  kurkumin  sesuai standar  pasar  serta  rendahnya keragaman  genetik  kunyit  sebagai  bahan  seleksi  pemuliaan konvensional menjadikan penelitian ini penting untuk dilakukan. Tujuan penelitian ini adalah untuk mendapatkan  dosis  LD50  serta  menghasilkan  perubahan  keragaan tanaman  kunyit  akibat  induksi mutasi  fisik  melalui  iradiasi  sinar  gamma. Iradiasi  dilakukan  secara  tunggal  (acute  irradiation) menggunakan Iradiator Panorama Serba Guna (IRPASENA) dengan 11 taraf dosis yang berbeda dan tanaman  yang  telah  diradiasi  dibudidayakan  secara  in  vivo. Pengamatan  pertumbuhan  tanaman kunyit  dilakukan  pada  karakter vegetatif secara  kuantitatif  dan  kualitatif.  Pada  penelitian  ini, didapatkan  LD50 kunyit  yaitu  pada  dosis  47.26  Gy.  Pertumbuhan  vegetatif  tanaman cenderung mengalami  perlambatan  dengan  semakin  meningkatnya  dosis iradiasi.  Keragaman  tertinggi pertumbuhan jumlah daun terdapat pada aplikasi dosis 50 Gy. Perubahan morfologi berupa bentuk pangkal  batang  semu  terjadi pada  tanaman  akibat  iradiasi  dosis  50  dan  60  Gy,  perubahan warna sebagian permukaan daun dan terjadi pada 50 dan 70 Gy, perubahan bentuk daun terjadi pada 50 dan 70 Gy, serta pertumbuhan tanaman yang kerdil terjadi pada tanaman 60 dan 70 Gy.</p><p>Kata kunci: iradiasi, kunyit, kurkumin, LD50</p>

scholarly journals Gamma Ray-Induced Polymerization and Cross-Linking for Optimization of PPy/PVP Hydrogel as Biomaterial

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 111 ◽  
Author(s):  
Jin-Oh Jeong ◽  
Jong-Seok Park ◽  
Young-Ah Kim ◽  
Su-Jin Yang ◽  
Sung-In Jeong ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cell Viability ◽  
Gamma Rays ◽  
Biomedical Applications ◽  
Gamma Ray ◽  
The Other ◽  
Cross Linking ◽  
Gamma Ray Irradiation ◽  
Good Cell ◽  
Scanning Electron

Conducting polymer (CP)-based hydrogels exhibit the behaviors of bending or contraction/relaxation due to electrical stimulation. They are similar in some ways to biological organs and have advantages regarding manipulation and miniaturization. Thus, these hydrogels have attracted considerable interest for biomedical applications. In this study, we prepared PPy/PVP hydrogel with different concentrations and content through polymerization and cross-linking induced by gamma-ray irradiation at 25 kGy to optimize the mechanical properties of the resulting PPy/PVP hydrogel. Optimization of the PPy/PVP hydrogel was confirmed by characterization using scanning electron microscopy, gel fraction, swelling ratio, and Fourier transform infrared spectroscopy. In addition, we assessed live-cell viability using live/dead assay and CCK-8 assay, and found good cell viability regardless of the concentration and content of Py/pTS. The conductivity of PPy/PVP hydrogel was at least 13 mS/cm. The mechanical properties of PPy/PVP hydrogel are important factors in their application for biomaterials. It was found that 0.15PPy/PVP20 (51.96 ± 6.12 kPa) exhibited better compressive strength than the other samples for use in CP-based hydrogels. Therefore, it was concluded that gamma rays can be used to optimize PPy/PVP hydrogel and that biomedical applications of CP-based hydrogels will be possible.

scholarly journals Study on Induced Current of Iron Plate Irradiated by Pulsed Gamma Rays

2018 ◽  
Vol 53 ◽  
pp. 01007
Author(s):  
Yinghong Zuo ◽  
Maoyu Zhang ◽  
Guoxin Cheng ◽  
Shengli Niu
Keyword(s):  
Gamma Rays ◽  
Gamma Ray ◽  
Transient Current ◽  
Current Response ◽  
Induced Current ◽  
Metal Component ◽  
Iron Plate ◽  
Gamma Ray Irradiation ◽  
Charge Deposition ◽  
The Relationship

To obtain the transient current response law of the metal component irradiated by pulsed gamma rays, the pulsed gamma ray irradiation experiment of the iron plate was carried out on “Qiangguang-I” accelerator. The transient current of iron plate generated by pulsed gamma rays was measured and analysed, and the relationship between the amplitude of pulse current and the dose rate of gamma rays was obtained. The results show that the current response sensitivity of the iron plate is about 5.7×10-7(A/m2)/(Gy/s) when the gamma rays with the energy of 0.8 MeV irradiate the iron plate. The charge deposition rate in the iron plate can be obtained by Monte Carlo simulation, and then it can be converted to gamma ray induced current of the metal component irradiated by gamma rays.

Effects of different genotypes and gamma ray doses on haploidization with irradiated pollen technique in watermelon (Citrullus lanatus L.)

2013 ◽  
Vol 93 (6) ◽  
pp. 1165-1168 ◽  
Author(s):  
Hatıra Taşkın ◽  
Namık Kemal Yücel ◽  
Gökhan Baktemur ◽  
Songül Çömlekçioğlu ◽  
Saadet Büyükalaca
Keyword(s):  
Gamma Rays ◽  
Irradiation Dose ◽  
Gamma Ray ◽  
Citrullus Lanatus ◽  
Embryo Rescue ◽  
Genotype 1 ◽  
Irradiated Pollen ◽  
Glass Tubes ◽  
Male Flowers ◽  
Haploid Embryos

Taşkın, H., Yücel, N. K., Baktemur, G., Çömlekçioğlu, S. and Büyükalaca, S. 2013. Effects of different genotypes and gamma ray doses on haploidization with irradiated pollen technique in watermelon ( Citrullus lanatus L.). Can. J. Plant Sci. 93: 1165–1168. Two watermelon genotypes, one commercial watermelon variety (Ustun F1) and five different doses of gamma rays coming from Co60 were tested to develop useful haploidization procedures in watermelon. For this purpose, male flowers collected a day before anthesis were irradiated with 50, 150, 200, 275 and 300 Gy doses of gamma rays, and female flowers were pollinated with irradiated pollen the next day. Seeds extracted from fruits harvested 25 d later were opened individually in a laminar flow hood. Embryos obtained via embryo rescue technique were placed in glass tubes containing CP medium with 30 g L−1 sucrose, 8 g L−1 agar, 0.08 mg L−1 B12, and 0.02 mg L−1 IAA. Sixty haploid embryos were obtained from 43 watermelon fruits in this study. Genotype 1 was found to be the most successful genotype with 3.57 haploid embryos per 100 seeds. Among tested irradiation doses, 275 Gy was better than other doses, with 5.26 haploid embryos per 100 seeds. Considered together with irradiation dose and genotypes, the maximum number of haploid embryos was obtained from Genotype 1 pollinated with 275 Gy irradiation dose, with 6.25 haploid embryos per 100 seeds.

Effects of Fabrication Process on the Electrical Characteristics of n-Channel MOSFETs Irradiated with Gamma-Rays

2008 ◽  
Vol 600-603 ◽  
pp. 707-710
Author(s):  
Shigeomi Hishiki ◽  
Naoya Iwamoto ◽  
Takeshi Ohshima ◽  
Hisayoshi Itoh ◽  
Kazu Kojima ◽  
...  
Keyword(s):  
Gamma Rays ◽  
Radiation Resistance ◽  
Carbon Coating ◽  
Gamma Ray ◽  
Sample Surface ◽  
Fabrication Process ◽  
Channel Mobility ◽  
Carbon Coated ◽  
Gamma Ray Irradiation ◽  
Post Implantation

The effect of the fabrication process of n-channel 6H-SiC MOSFETs on their radiation resistance is investigated. MOSFETs that post implantation annealing for source and drain was carried out with carbon coating on the sample surface are compared to MOSFETs that post implantation annealing was carried out without carbon coating. The radiation resistance (gamma-rays) of the carbon-coated MOSFETs is higher than that of non-coated ones. The channel mobility for MOSFETs whose gate oxide was formed using pyrogenic or dry oxidation process dose not change by gamma-ray irradiation below 1x105 Gy. The value of channel mobility slightly increases with increasing dose above 1x105 Gy. No significant increase in irradiation induced interface traps is observed.

scholarly journals Evaluasi Keragaman Genetik Mutan Harapan Generasi MV1 Jeruk Keprok SoE (Citrus reticulata Blanco) Berdasarkan Penanda Morfologi dan ISSR

2016 ◽  
Vol 7 (2) ◽  
pp. 102
Author(s):  
Indriati Husain ◽  
Agus Purwito ◽  
Ali Husni ◽  
Kikin H. Mutaqin ◽  
Slamet Susanto
Keyword(s):  
Genetic Diversity ◽  
Gamma Irradiation ◽  
Genetic Distance ◽  
Embryogenic Callus ◽  
Irradiation Dose ◽  
Gamma Ray ◽  
Morphological Diversity ◽  
Issr Markers ◽  
Citrus Reticulata ◽  
Gamma Ray Irradiation

<p class="Abstrak"><em>ABSTRACT</em><em></em></p><p><em>Mandarin’s SoE is national <del cite="mailto:Windows%207" datetime="2016-10-31T15:54"></del>variety originated <del cite="mailto:Windows%207" datetime="2016-10-31T15:55"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins>from Mount of Mutis, Sub District of SoE, of Timur Tengah Selatan (TTS) District, East Nusa Tenggara (NTT). The genetic diversity of citrus can be induced by gamma ray irradiation on embryogenic callus cells thus producing new mutants. <del cite="mailto:Windows%207" datetime="2016-10-31T15:55"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins><del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del>Genetic diversity detection can be <ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>based on morphological and ISSR markers. The aim of this research was <del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>to obtain information on the genetic diversity <del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>on putative mutants<ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins> mandarin SoE induced by <ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>gamma ray irradiation <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del>based on morphology and markers ISSR. ISSR markers used are ISSR 1, 4, 6 and 8. Analysis of morphological diversity produced <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>a dendrogram with the level of similarity between individuals each irradiation dose 83-95% with <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>5-17% genetic distance. Dendrogram analysis based on the genetic diversity ISSR markers showed high levels of 51-100% similarity and genetic distance 0-49%. Individuals<del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del> samples obtained from<del cite="mailto:Windows%207" datetime="2016-10-31T15:58"></del><del cite="mailto:Windows%207" datetime="2016-10-31T15:58"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:58"></ins> gamma irradiation, based <ins cite="mailto:Windows%207" datetime="2016-10-31T15:58"></ins>both morphological and ISSR markers, was different from individual's genetic make up before irradiation.</em></p><p class="Abstrak"><em>Keywords</em><em>:</em><em> cluster, </em><em>gamma ray</em><em>, genetic distance, genetic diversity</em><em>s, </em><em>similarity</em></p><p class="Abstrak"> </p><p class="Abstrak">ABSTRAK</p><p>Jeruk keprok SoE adalah jeruk varietas unggul nasional yang berasal dari Pegunungan Mutis, Kecamatan SoE, Kabupaten Timur Tengah Selatan (TTS), Provinsi Nusa Tenggara Timur (NTT). Keragaman genetik jeruk ini dapat diinduksi dengan iradiasi sinar gamma pada sel-sel kalus embriogenik untuk menghasilkan mutan yang solid. Deteksi keragaman genetik yang terbentuk dapat dilakukan secara morfologi maupun molekuler dengan marka ISSR. Tujuan Penelitian ini adalah untuk mendapatkan informasi mengenai keragaman genetik yang terjadi pada mutan harapan jeruk keprok SoE hasil iradiasi sinar gamma berdasarkan morfologi dan marka ISSR. Marka ISSR yang digunakan adalah ISSR 1, 4, 6 dan 8 pada beberapa mutan harapan jeruk keprok SoE. Analisis keragaman secara morfologi menghasilkan dendrogram dengan tingkat kemiripan antar individu masing-masing dosis iradiasi 83-95% dengan jarak genetik 5-17%. Dendrogram analisis keragaman genetik berdasar marka ISSR memperlihatkan tingkat kemiripan 51-100% dan jarak genetik 0-49%. Individu-individu sampel yang diuji hasil iradiasi gamma, baik secara morfologi dan marka ISSR, telah memiliki susunan genetik yang berbeda dari susunan genetik individu sebelum diiradiasi.</p><p>Kata kunci: grup, jarak genetik, kemiripan, keragaman, sinar gamma</p>

Oxygen Ion Induced Charge in SiC MOS Capacitors Irradiated with Gamma-Rays

2011 ◽  
Vol 679-680 ◽  
pp. 362-365 ◽  
Author(s):  
Takeshi Ohshima ◽  
Naoya Iwamoto ◽  
Shinobu Onoda ◽  
Takahiro Makino ◽  
Shinji Nozaki ◽  
...  
Keyword(s):  
Gamma Rays ◽  
Gamma Ray ◽  
Ion Beam ◽  
Gate Oxide ◽  
Peak Amplitude ◽  
Induced Current ◽  
Mos Capacitors ◽  
Oxygen Ion ◽  
Before And After ◽  
Gamma Ray Irradiation

Charge induced in 6H-SiC nMOS capacitors by 15 MeV oxygen ion microbeams was measured using Transient Ion Beam Induced Current (TIBIC) before and after gamma-ray irradiations. The peak amplitude of TIBIC signals decreases and the fall time increases with increasing number of incident ions. The decrease in the TIBIC peak eventually saturated. The TIBIC signal can be refreshed to its original shape by applying a positive bias of + 1V to gate oxide. Small decrease in both the peak amplitude of TIBIC signal and collected charge was observed due to gamma-ray irradiation.

scholarly journals THE ADAPTATION LEVEL OF ALFALFA (MEDICAGO SATIVA L.) THAT IRRADIATED WITH GAMMA RAYS ON A FIELD SCALE

Pastura ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
Prihantoro, I. ◽  
Anandia, A. ◽  
Aryanto, A. T. ◽  
Setiana, M. A. ◽  
Karti, P. D. M. H.
Keyword(s):  
Medicago Sativa ◽  
Gamma Rays ◽  
Gamma Ray ◽  
Adaptation Level ◽  
Field Scale ◽  
Leaf Color ◽  
Randomized Design ◽  
Gamma Ray Irradiation ◽  
Medicago Sativa L ◽  
Dark Green

Alfalfa (Medicago sativa L.) a high nutritious and palatability legume for ruminant. Constrain of alfalfa availability in Indonesia are due to the plant adaptability in tropical environment. Aim of the study was to measure the adaptation level of alfalfa that irradiated with gamma rays on a field scale. The study consisted of four types of plant sources, mutation plants with different gamma ray levels (0Gy, 200Gy, 300Gy and 400Gy). Plant tillers was analyzed with a complete randomized design with 3 replications, 20 plants per replication. Growth capability, leaf color, flowering time and pest attack level were analyzed descriptively. The results showed that alfalfa irradiated with 300 Gy significantly (p<0.05) produced highest tillers. Irradiated 300 Gy plant gave better result on growth capability, flowering capability, and number of not attack plant from pests. The higher level of gamma ray irradiation showed the changed in leaf color levels from dark green to light green. Keywords: alfalfa (Medicago sativa L.), mutation, gamma ray irradiation, field scale

scholarly journals Mutagenic Effect of Proton Beams Characterized by Phenotypic Analysis and Whole Genome Sequencing in Arabidopsis

2021 ◽  
Vol 12 ◽  
Author(s):  
Sang Woo Lee ◽  
Yu-Jeong Kwon ◽  
Inwoo Baek ◽  
Hong-Il Choi ◽  
Joon-Woo Ahn ◽  
...  
Keyword(s):  
Proton Beam ◽  
Gamma Rays ◽  
Gamma Ray ◽  
Mutagenic Effect ◽  
Mutation Rates ◽  
Whole Genome ◽  
Structural Variations ◽  
Proton Beam Irradiation ◽  
Proton Beams ◽  
Gamma Ray Irradiation

Protons may have contributed to the evolution of plants as a major component of cosmic-rays and also have been used for mutagenesis in plants. Although the mutagenic effect of protons has been well-characterized in animals, no comprehensive phenotypic and genomic analyses has been reported in plants. Here, we investigated the phenotypes and whole genome sequences of Arabidopsis M2 lines derived by irradiation with proton beams and gamma-rays, to determine unique characteristics of proton beams in mutagenesis. We found that mutation frequency was dependent on the irradiation doses of both proton beams and gamma-rays. On the basis of the relationship between survival and mutation rates, we hypothesized that there may be a mutation rate threshold for survived individuals after irradiation. There were no significant differences between the total mutation rates in groups derived using proton beam or gamma-ray irradiation at doses that had similar impacts on survival rate. However, proton beam irradiation resulted in a broader mutant phenotype spectrum than gamma-ray irradiation, and proton beams generated more DNA structural variations (SVs) than gamma-rays. The most frequent SV was inversion. Most of the inversion junctions contained sequences with microhomology and were associated with the deletion of only a few nucleotides, which implies that preferential use of microhomology in non-homologous end joining was likely to be responsible for the SVs. These results show that protons, as particles with low linear energy transfer (LET), have unique characteristics in mutagenesis that partially overlap with those of low-LET gamma-rays and high-LET heavy ions in different respects.

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