Perilaku Biokimia Benih Kedelai Selama Penyimpanan dalam Kondisi Terkontrol

ABSTRACT Physiological and biochemical changes in seeds indicates levels of viability and vigor during deterioration. The aims of the research were to study the patterns of biochemical changes in soybean seeds and its relationship to seed vigor during storage under controlled conditions. The experiment was arranged in a completely randomized design using two varieties as a factor which was nested into storage period and replicated four times. Two varieties were Gepak Kuning and Mallika, stored in controlled condition at temperature of 19-22 °C and 64-67% of relative humidity for six months. The seeds were evaluated for protein, peroxide value, electrical conductivity and seed viability. The exponential regression analysis was used to determine the patterns of biochemical changes of each soybean varieties during the storage period. The results showed that the biochemical behaviour of soybean seeds stored under controlled conditions for 24 weeks following the exponential equation of y = a + b expcx, which a, b, and c were constants that has different value for each variety. Electrical conductivity and peroxide value could be used as indicators of seed vigor during storage under controlled conditions for 24 weeks. Seed storage under controlled conditions are to maintain seed vigor >80% up to 12 weeks for Mallika and 24 weeks for Gepak Kuning with low oxidative process and less membrane damages. Keywords:deterioration, storability, vigor

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Temperature during soybean seed storage and the amount of electrolytes of soaked seeds solution

Scientia Agricola ◽

10.1590/s0103-90162008000500008 ◽

2008 ◽

Vol 65 (5) ◽

pp. 496-501 ◽

Cited By ~ 6

Author(s):

Roberval Daiton Vieira ◽

Dennis Merlin TeKrony ◽

Dennis Bruce Egli ◽

William Patrick Bruenning ◽

Maristela Panobianco

Keyword(s):

Electrical Conductivity ◽

Storage Temperature ◽

Soybean Seed ◽

Storage Period ◽

Seed Storage ◽

Seed Vigor ◽

Soybean Seeds ◽

Glycine Max L ◽

Pea Seeds ◽

And Storage

The electrical conductivity test measures the electrolytes that leach out of seeds when they are immersed in water and this leakage is an indication of seed vigor. The level of standardization reached by the procedures of this test is such that the test is recommended for pea seeds and suggested for other large seeded legumes, including soybean [Glycine max (L.) Merrill]. This study was conducted to contribute to the standardization of this test for soybean seeds by verifying whether the seed storage temperature influences the composition of the leachate from soaked seeds solution. Two soybean seed lots of distinct physiological potential were stored in moisture-proof containers either at constant temperatures of 10ºC and 20ºC or at the temperature of 20ºC during the first seven months of storage followed by a change to 10ºC for the rest of the storage time (nine months). The chemical composition of the soaked water was evaluated every three months from January to October 1998. The highest amount of leakage was observed for potassium, followed by calcium and magnesium, iron and sodium regardless of temperature and storage period. The amount of electrolytes in the soaked water increased as the period of time and the temperature of storage increased. On the other hand the amount of leakage decrease along the time for those seeds stored at 10ºC or transferred from the temperature of 20 to that of 10ºC. The temperature at which soybean seeds remain during storage may affect the amount of electrolytes in the soaked water and consequently the results of the electrical conductivity test.

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Efektivitas Seed Coating dan Biopriming dengan Rizobakteri dalam Mempertahankan Viabilitas Benih Cabai dan Rizobakteri selama Penyimpanan

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.8.3.192-202 ◽

2018 ◽

Vol 8 (3) ◽

pp. 192

Author(s):

Ita Madyasari ◽

Candra Budiman ◽

Syamsuddin , ◽

Dyah Manohara ◽

Satriyas Ilyas

Keyword(s):

Sodium Alginate ◽

Seed Treatment ◽

Seed Viability ◽

Storage Period ◽

Seed Vigor ◽

Hot Pepper ◽

Seed Coating ◽

Control Seed ◽

Randomized Design ◽

Two Factors

ABSTRACTThe objective of the study was to obtain the best coating formula for hot pepper seeds, and evaluate the effect of seed coating and biopriming with rhizobacteria on viability of hot pepper seeds and rhizobacteria during storage. Experiment 1 was arranged in a completely randomized design with one factor i.e. 11 coating formula. Experiment 2 was arranged in a nested plot design with two factors, storage period (0, 4, 8, 12, 16, 20, and 24 weeks) as main factor and seed treatment consisted of 11 treatments (control, seed coating with E1+F2B1, ST116B, CM8; biopriming 24 h with E1+F2B1, ST116B, CM8; biopriming 48 h with E1+F2B1, ST116B, and CM8; priming metalaxyl) as nested factor. Result of experiment 1 indicated that the best coating formula for hot pepper seed was sodium alginate 2.5% and was used in experiment 2. Experiment 2 showed that seed coating and biopriming with rhizobacteria were able to maintain seed viability (79-89%) for 24 weeks of storage at 27-30 0C as compared to priming metalaxyl (54%). Biopriming E1+F2B1 24 h or CM8 48 h resulted in the highest index of seed vigor after 24 weeks of storage. Population of rhizobacteria in seed tissue decreased in bioprimed seeds from 105-107 cfu g-1 to 104 cfu g-1 after being stored for 24 weeks. Keywords: rhizobacteria isolates, seed treatment, seed vigor, sodium alginate ABSTRAK Penelitian ini bertujuan mendapatkan formula coating terbaik pada benih cabai dan mengevaluasi pengaruh seed coating dan biopriming dengan rizobakteri dalam mempertahankan viabilitas benih cabai dan rizobakteri selama penyimpanan. Percobaan 1 menggunakan rancangan acak lengkap satu faktor yang terdiri atas 11 formula coating. Percobaan 2 menggunakan rancangan petak tersarang dua faktor, periode simpan (0, 4, 8, 12, 16, 20, dan 24 minggu) sebagai faktor utama dan perlakuaan benih yang terdiri atas 11 perlakuan (kontrol, seed coating dengan E1+F2B1, ST116B, CM8; biopriming 24 jam dengan E1+F2B1, ST116B, CM8; biopriming 48 jam dengan E1+F2B1, ST116B, dan CM8; priming metalaksil) sebagai faktor tersarang. Hasil Percobaan 1 menunjukkan bahwa formula coating terbaik untuk benih cabai ialah natrium alginat 2.5% dan digunakan pada percobaan 2. Percobaan 2 menunjukkan bahwa seed coating dan biopriming dengan rizobakteri mampu mempertahankan viabilitas benih (78-89%) selama 24 minggu penyimpanan pada suhu 27-30 0C dibandingkan priming metalaksil (54%). Biopriming E1+F2B1 24 jam atau biopriming CM8 48 jam menghasilkan indeks vigor paling tinggi setelah disimpan selama 24 minggu. Populasi rizobakteri di dalam jaringan benih menurun pada benih yang diberi perlakuan biopriming dari 105-107 cfu g-1 menjadi 104 cfu g-1 setelah disimpan selama 24 minggu.Kata kunci: isolat rizobakteri, natrium alginat, perlakuan benih, vigor

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Perubahan Biokimia dan Indikator Vigor Benih Kakao pada Lima Taraf Lamanya Penyimpanan

Jurnal Tanaman Industri dan Penyegar ◽

10.21082/jtidp.v7n3.2020.p163-178 ◽

2020 ◽

Vol 7 (3) ◽

pp. 163

Author(s):

Iing Sobari ◽

Sumadi Sumadi ◽

Santi Rosniawaty ◽

Edi Wardiana

Keyword(s):

Storage Period ◽

Seed Storage ◽

Maximum Growth ◽

Seed Vigor ◽

Growth Potential ◽

Hypocotyl Length ◽

Storage Duration ◽

Randomized Design ◽

Food Reserves ◽

Germinated Seeds

During the storage period, cocoa seeds undergo various processes that will affect the content of their food reserves and will have an impact on germination capacity and seeds vigor. The objective of this study is to investigate the changes of biochemical and vigor of cocoa seeds after storage for a maximum of 4 weeks with an interval of 1 week. The experiment was conducted at the Integrated Laboratory and Greenhouse of Balittri, Sukabumi, West Java, from October to December 2019. The completely randomized design with five treatments of seeds storage duration: (a) seeds unstored, (b) seeds stored for 1 week, (c) seeds stored for 2 weeks, (d) seeds stored for 3 weeks, and (e) seeds stored for 4 weeks, and five replications were used in this study. Parameters observed during seed storage were the biochemistry change of seeds and the percentage of germinated seeds, while during seed germination is an indicator of seed vigor. The results showed that storing cocoa seeds for 1-4 weeks decreased the fat content of the seeds, while the free fatty acids, protein, and percentage of germinated seeds increased. Until the 21st day after sowing, seed storage increases electrical conductivity but decreases growth rate, maximum growth potential, viability, and hypocotyl length. Seeds stored for one week still good in their vigor (viability of 80%), while seeds stored for 2-4 weeks have decreased their vigor (maximum viability of 54.4%).

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Assessment of physiological potential of stored pea (Pisum sativum L.) seeds

Journal of Seed Science ◽

10.1590/s2317-15372013000100006 ◽

2013 ◽

Vol 35 (1) ◽

pp. 42-50 ◽

Cited By ~ 1

Author(s):

Fábio Mielezrski ◽

Julio Marcos-Filho

Keyword(s):

Electrical Conductivity ◽

Growth Habit ◽

Seedling Emergence ◽

Accelerated Aging ◽

Seed Storage ◽

Seed Vigor ◽

Seedling Length ◽

Pea Seeds ◽

Cold Chamber ◽

Pea Seed

Research on pea seed storage and the identification of efficient seed vigor tests for this species is still insufficient, especially for cultivars that produce wrinkled seeds used for consumption in natura. The objective of this study was to verify the accuracy of tests to assess the physiological potential of pea seed lots stored in different environments. Four seed lots of two cultivars, 'Telefone Alta' (indeterminate growth habit) and 'Itapuã' (determinate growth habit) were stored under different environmental conditions for 8 months: a) laboratory uncontrolled b) dry and cold chamber (10 ºC and 30% RH), c) controlled environment (20 ºC and 70% RH). Seed vigor (accelerated aging, electrical conductivity, seedling length and seedling emergence) was evaluated initially and after four and eight months storage. Results showed that the use of seed lots of high initial physiological potential is fundamental for guaranteeing the desired performance of pea seeds during storage. Wrinkled pea seeds should be stored between harvest and sowing in an environment in which the sum of the relative humidity (%) and temperature (°C) does not exceed 70. Physiological potential of pea seeds should be evaluated by at least two vigor tests; consideration to accelerated aging and electrical conductivity tests is recommended.

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Influence of lignin content in soybean seed coat on the incidence of the storage fungus Aspergillus flavus

Revista Brasileira de Sementes ◽

10.1590/s0101-31222012000400003 ◽

2012 ◽

Vol 34 (4) ◽

pp. 541-548 ◽

Cited By ~ 2

Author(s):

Ísis Barreto Dantas ◽

João Almir de Oliveira ◽

Heloisa Oliveira dos Santos ◽

Édila Vilela Resende Von Pinho ◽

Sttela Dellyzete Veiga Franco da Rosa

Keyword(s):

Electrical Conductivity ◽

Aspergillus Flavus ◽

Seed Coat ◽

Seed Quality ◽

Lignin Content ◽

Soybean Seed ◽

Storage Period ◽

Seed Storage ◽

Seed Health ◽

Physiological Quality

Seed quality may be affected by several factors, including permeability, color, and lignin content in the seed coat. This study aimed at evaluating influence of lignin content in the tegument of seed samples of six different soybean cultivars, in which half of each sample was inoculated with the fungus Aspergillus flavus, on the physical and physiological quality, and on the seed health, during 180 days storage period, under cold chamber with controlled conditions of temperature and RH. For that, at each interval of 60 days, samples were removed, and the physiological quality of these seeds was assessed by means of moisture and lignin contents; and by tests of seed health, germination, and electrical conductivity. The moisture content of seeds remained constant during all storage period. In the seed health test, it was found that inoculation was efficient, once the minimum incidence of the fungus in the inoculated seeds was 85%. In the germination test, there was a trend of reduction on percentage germination with the increase in storage period. However, there was an increase on electrical conductivity of seeds assessed. It was concluded that there is no interference of the lignin content in the seed coat on the resistance to infection by the fungus Aspergillus flavus, even after seed storage for a period of 180 days.

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Biological features of seeds of a wild-growing species of Poterium polygamum

Agrarian science ◽

10.32634/0869-8155-2021-354-11-12-97-99 ◽

2022 ◽

Vol 354 (11-12) ◽

pp. 97-99

Author(s):

M. A. Starostina ◽

N. G. Lapenko

Keyword(s):

Natural Population ◽

Seed Viability ◽

Storage Period ◽

Seed Storage ◽

Scientific Basis ◽

The Third ◽

Biological Features ◽

High Germination ◽

Grass Stand ◽

The Subject

Relevance. Biological features (morphometric indicators, viability and germinating power) of Poterium polygamum seeds found in natural communites of the Stavropol Territory have been studied. The conservation of wild-growing plant species in the natural population and the rational use of their potential are relevant.Methods. Years of study — 2013–2021. The subject of the study is Poterium polygamum seeds. Seeds were collected in 2013. Studies were carried out according to the methods of studying seed material. Freshly harvested seeds and seeds of different shelf life were germinated in petri dishes at a temperature of 18–20 °C.Results. The collected seeds of Poterium polygamum are quite large. The length of the seeds is 4–5 mm, the width is about 2.5 mm. The mass of 1000 pieces of seeds on average was 10.59 grams. Complete absence of viability of freshly harvested Poterium polygamum seeds was revealed. After three months, seed viability was 97%. This is a shallow physiological rest associated with the post-harvest maturation period. During the storage of seeds, their high germination was established — from 70 to 100%. The duration of seed storage is 8 years. The largest number of germinated seeds and their germinating power were noted in the third year of their storage (100%). Seeds germinated on the third or fourth day. The differences between the viability of seeds and their germinating power are not great. In the eighth year of storage of Poterium polygamum seeds, viability and germinating power were slightly reduced. The storage period of seeds, in which they retain the ability to germinate and the germinating power, is long. It has been found that the wild species Poterium polygamum belongs to plants with a high (70 to 100%) viability. This species is regularly resumed in the natural population. The revealed biological features of Poterium polygamum seeds can become the scientific basis during the reconstruction of degraded natural grass stand and in selection work.

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Pola Penurunan Viabilitas dan Pengembangan Metode Pendugaan Vigor Daya Simpan Benih Pepaya (Carica Papaya L.)

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.7.3.146-154 ◽

2017 ◽

Vol 7 (3) ◽

pp. 146

Author(s):

Astryani Rosyad ◽

M. Rahmad Suhartanto ◽

Abdul Qadir

Keyword(s):

Moisture Content ◽

Aging Time ◽

Seed Quality ◽

Initial Moisture Content ◽

Accelerated Aging ◽

Storage Period ◽

Seed Storage ◽

Seed Vigor ◽

Develop Model ◽

Papaya Seed

ABSTRACT Information of seed quality during storage can be determined through the actual storage and storability vigor estimation. This study aimed at comparing effective accelerated aging method between physical and chemical, and studying the seed deterioration during storage in ambient (T =28-30 0C, RH=75-78%) and AC (T =18-20 0C, RH =51-60%) condition with three levels of initial moisture content (8-10%, 10-12%, and 12-14%) for 20 weeks. The final objective of this research was to develop model for storability vigor of papaya seed. Two experiments, accelerated aging and seed storage were conducted at Seed Laboratory, Department of Agronomy and Horticulture, Bogor Agricultural University from October 2015 to May 2016. A completely randomized design with nested factors and four replications was applied to both experiments. The results showed that physical accelerated aging using IPB 77-1 MMM machine was more effective than chemical accelerated aging using IPB 77-1 MM machine for papaya seed. The viability of seed stored in AC condition remained high until the end of the storage period, whereas it declined at 16 week storage period in the ambient condition. The viability of seed with initial moisture content of 12-14% declined faster than that of initial moisture content of 8-10% after 18 week storage periode. The model used to estimate the storability vigor of papaya seed accurately was the equation y = a + b expcx where y : storability vigor estimation, x : aging time and a,b,c : constant value. Simulation of storability vigor estimation with constant value of a, b, c and input of aging time can estimate storability seed vigor in actual storage. Keywords: accelerated aging, IPB 77-1 MM machine, IPB 77-1 MMM machine, seed storage, simulationABSTRAK Informasi mutu benih selama penyimpanan dapat diketahui melalui penyimpanan secara aktual dan pendugaan vigor daya simpan. Penelitian ini bertujuan untuk membandingkan metode pengusangan cepat yang efektif antara fisik dengan kimia serta mempelajari pola penurunan viabilitas benih selama penyimpanan aktual pada kondisi simpan kamar (suhu =28-30 0C, RH =75-78%) dan AC (suhu =18-20 0C, RH =51-60%) dengan tiga tingkat kadar air awal (8-10%, 10-12%, dan 12-14%) selama 20 minggu. Tujuan akhirnya adalah membangun model vigor daya simpan benih pepaya. Penelitian pengusangan cepat dan penyimpanan dilakukan pada bulan Oktober 2015 sampai Mei 2016 di Laboratorium Benih, Departemen Agronomi dan Hortikultura, Institut Pertanian Bogor. Kedua penelitian menggunakan rancangan acak lengkap tersarang dengan empat ulangan. Hasil penelitian menunjukkan bahwa pengusangan cepat secara fisik dengan alat IPB 77-1 MMM lebih efektif daripada pengusangan kimia dengan alat IPB 77-1 MM untuk benih pepaya. Viabilitas benih yang disimpan pada kondisi AC tetap tinggi hingga akhir periode simpan, sedangkan pada kondisi kamar penurunan viabilitas dimulai pada periode simpan 16 minggu. Benih yang disimpan dengan tingkat KA awal sebesar 12-14% lebih cepat mengalami penurunan viabilitas mulai periode simpan 18 minggu dibandingkan dengan benih dengan KA awal 8-10%. Hasil penelitian juga menunjukkan terdapat korelasi yang erat antara pola kemunduran benih pada pengusangan cepat dan penyimpanan aktual, sehingga model pendugaan vigor daya simpan (y) berdasarkan waktu pengusangan (x) dapat disusun dengan persamaan y = a + b expcx. Simulasi pendugaan vigor daya simpan dengan nilai konstanta a, b, dan c serta input waktu pengusangan dapat menduga vigor daya simpan benih selama penyimpanan aktual. Kata kunci: alat IPB 77-1 MM, alat IPB 77-1 MMM, pengusangan cepat, penyimpanan benih, simulasi

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Is it possible to estimate longevity through the analyses used to measure the initial physiological potential in soybean seeds?

Journal of Seed Science ◽

10.1590/2317-1545v43249697 ◽

2021 ◽

Vol 43 ◽

Author(s):

Thiago Barbosa Batista ◽

Samara Moreira Perissato ◽

Carlos Henrique Queiroz Rego ◽

Gustavo Roberto Fonseca de Oliveira ◽

Fernando Augusto Henning ◽

...

Keyword(s):

Electrical Conductivity ◽

Linear Regression ◽

Soybean Seed ◽

Accelerated Aging ◽

Storage Period ◽

High Potential ◽

Seed Longevity ◽

Soybean Seeds ◽

Radicle Protrusion ◽

Over Time

Abstract: The analysis of longevity can support decisions about the length of seed lot storage until commercialization, since this characteristic implies the maintenance of viability over time. Seed longevity is analyzed by the p50 test, which expresses the time to lose 50% of the initial viability. Seeds with high vigor and germination have greater physiological potential and, thus, a greater capacity to maintain quality throughout the storage period. However, there has been little research on the correlations between the analysis of p50 (longevity) and the tests used to measure physiological potential (germination and vigor) of lots, which can be used as a tool to make inferences about longevity using the most traditional tests. Thus, the objective of this study was to investigate which tests used to measure the potential of lots can estimate p50. To this end, germination and vigor were evaluated using traditional tests while longevity was assessed in eight soybean seed lots. Correlations and linear regression were tested for the traditional variables versus p50. It was found that the use of accelerated aging, electrical conductivity, and time to 50% radicle protrusion has high potential to estimate longevity as measured by p50.

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Aplikasi Bakteri Probiotik untuk Meningkatkan Mutu Fisiologi dan Kesehatan Bibit Cabai (Capsicum annuum L.)

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) ◽

10.24831/jai.v44i2.13487 ◽

2016 ◽

Vol 44 (2) ◽

pp. 176 ◽

Cited By ~ 1

Author(s):

Anna Tefa ◽

Eny Widajati ◽

Muhamad Syukur ◽

Dan Giyanto

Keyword(s):

Storage Period ◽

Seed Storage ◽

Colletotrichum Acutatum ◽

Seed Coating ◽

Pseudomonas Sp ◽

Bacillus Sp ◽

Seedling Vigour ◽

Chilli Pepper ◽

Control Seed ◽

Randomized Design

ABSTRACT The use of probiotic bacteria as biocontrol agents is one of the methods of controlling anthracnose disease caused by Colletotrichum acutatum. The objective of this research was to suppress the infection of C. acutatum and increase chilli pepper seedling vigour. The research involved factorial experiments arranged in a completely randomized design with two factors. The first factor was seed coating involving six treatments, i.e., control, seed coating without bacteria, seed coating with Bacillus sp., Pseudomonas sp., Actinomycetes sp, and fungicide. The second factor was the seed storage period where six storage periods were experimented, i.e., 0, 1, 2, 3, 4, 5, and 6 months. The results showed that the coating treatment of Bacillus sp., Pseudomonas sp., and Actinomycetes sp. improved germination, growth rate and number of leaves and reduced the incidence of attacks and infection of hypocotyls at 5 month storage period. Keywords: Actinomycetes sp., Bacillus sp., Pseudomonas sp., seed coating, storage period

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Reserve mobilization during imbibition of stored Gliricidia sepium (Jacq.) Steud. (Leguminosae - Papilionoideae) seeds

Revista Brasileira de Sementes ◽

10.1590/s0101-31222011000300018 ◽

2011 ◽

Vol 33 (3) ◽

pp. 549-560

Author(s):

Renata Conduru Ribeiro Reis ◽

Bárbara França Dantas ◽

Renato Delmondez de Castro ◽

Cimille Gabrielle Cardoso Antunes ◽

Fabrício Francisco Santos da Silva ◽

...

Keyword(s):

Soluble Sugars ◽

Seed Viability ◽

Storage Period ◽

Seed Storage ◽

Gliricidia Sepium ◽

Storage Conditions ◽

Ambient Conditions ◽

Total Soluble Sugars ◽

Drought Tolerant ◽

Semi Arid Region

Gliricidia sepium is a drought-tolerant species, easily multiplied by seeds, and has been exploited by farmers as a source of forage in the semi-arid region of northeast Brazil. The objective of the present study was to evaluate the effect of seed storage on the mobilization of reserves during imbibition of "Gliricidia" seeds. Freshly-harvested seeds were packed in kraft paper bags and stored for three and six months in the laboratory under ambient conditions (25 º C ± 3 T and 75% ± 3 RH). Cotyledons were isolated from imbibed seeds and macerated for the extraction and quantification of total soluble sugars, reducing sugars, sucrose and starch, as well as of proteins, amino acids and for amylase activity. Storage under these conditions resulted in an increase in seed water content although germination remained at relatively high levels (86%). Seed macromolecule levels showed significant variation with the storage period and imbibition and these variations were associated with a loss in seed viability due to inadequate storage conditions.

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