scholarly journals PENETAPAN RHODAMIN B PADA SAMPEL LIPSTIK DENGAN MENGGUNAKAN KLT-SPEKTROFOTODENSITOMETRI

Jurnal Kimia ◽  
2020 ◽  
pp. 77
Author(s):  
N.N.A.S. Devi ◽  
N.P.M.P.P. Winarni ◽  
I.P. Priyasana ◽  
G.A.D. Mayagita ◽  
V. Rahmadinha ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Silica Gel ◽  
Liver Damage ◽  
Rhodamine B ◽  
Mobile Phase ◽  
Uv Light ◽  
Lymph Glands ◽  
Red Color ◽  
Rhodamine B Dye ◽  
Rhodamin B

Lipsticks are widely used by women to beautify themselves. Among the various colors that make lipstick more interesting, red color lipstick is the most demanded one. Rhodamine B is a synthetic dye that banned for use and certified as a hazardous material according to Minister of Health of Indonesian Republic No. 376/Menkes/Per/1990 because it causes liver damage, kidney and lymph glands damage, followed with organ enlargement. The aim of this study is to identify Rhodamine B in lipsticks in the market. Samples were taken from 3 shops in Denpasar City and Badung Regency. Samples were soaked with amonia solution with using wool yarn to extract the rhodamine B dye and identification using TLC plate silica gel GF254 withn-butanol: ethyl acetate: amonia (1322:5.2:6.5) as mobile phase then detected with UV light 254 and 366 nm. Identification by spectrophotodensitometry where the TLC plate was observed in the TLC Analyzer to observe the AUC in each spot formed. The AUC obtained from the instrument illustrates the concentration of the analyte in each bottle. The result shows that 3 examined samples doesn’t contain rhodamine B.   Keywords: Rhodamin B, Lipstik, TLC, Spectrophotodensitometry

scholarly journals Al-Qur�an dan Sains?(Bukti Kebenaran dalam Kasus Propolis Madu)

2014 ◽  
Vol 12 (1) ◽  
Author(s):  
MF. Zenrif Dan Erna Susanti
Keyword(s):  
Free Radicals ◽  
Liver Damage ◽  
Rhodamine B ◽  
Therapy Group ◽  
Carcinogenic Effect ◽  
Treatment Groups ◽  
Group 3 ◽  
Group 2 ◽  
Dose Levels ◽  
Rhodamin B

Exposure free radicals in the activity of this life can not be avoided, included exposure free radicals from food additive espesifically Rhodamine B. Carcinogenic effect of Rhodamin B can be caused by Poly Aromatic Hydrocarbons structure that are destructive to the liver as metabolism organ. The purpose of this study was to prove the antioxidant activity of Honey Propolis against liver damage caused by exposure of Rhodamine B as free radical sources. Indicator of liver damage can be indicated by MDA, a marker of oxidative stress that occurs in these organs. The methode was used experimental methode, with seven treatment groups, namely: (1) normal group, (2) the induction of Rhodamin B without propolis therapy group, (3) the induction of Rhodamin B with Honey Propolis therapy with three dose levels are 350 mg, 700 mg and 1050 mg that are given at intervals of two hours after the administration of Rhodamine B (4) Honey Propolis therapy groups are given at interval of 1 week aftertheadministrationof RhodamineBwiththreedoselevels.MDAlevelsweremeasuredbyTBARS method. The results showed that the levels of Malondialdehyde declined steadily in the group treated with honey propolis to less than control groups. There are significant differences in MDA levels between the treatment groups Honey Propolis therapy (p = 0.034, p <0.05). Based on these results, it can be concluded that Honey propolis dose 700 mg act as therapeutic agent in liver damage due to exposure of Rhodamine B significantly.The results ofthis studyprove thetruth ofthe Quraninthe QS. Al-Nahl (16): 69of thewonders ofhoneyin preventingliver damagedue toexposure tohazardous substancesin food.

scholarly journals PEMANFAATAN LIMBAH KULIT BUAH PISANG KEPOK (Musa paradisiaca) SEBAGAI BIOSORBEN ZAT WARNA RHODAMIN B

KOVALEN ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 308-314
Author(s):  
Musafira Musafira ◽  
Nurfitrah M Adam ◽  
Dwi Juli Puspitasari
Keyword(s):  
Adsorption Capacity ◽  
Rhodamine B ◽  
Contact Time ◽  
Banana Peel ◽  
Musa Paradisiaca ◽  
Randomized Design ◽  
Rhodamine B Dye ◽  
Completely Randomized Design ◽  
Maximum Contact ◽  
Rhodamin B

The investigation about the utilization of Banana peel (Musa paradisiaca) as biosorbent Rhodamine B dye has been done The purpose of this study was to determine the maximum contact time and to determine the adsorption capacity of kepok banana peel. Completely randomized design (CRD) was used in this research with two variables (the contact time and Rhodamine B concentration. Both variables were done in five levels i.e 10, 30, 60, 90, and 120 min and 2, 4, 6, 8 and 10 ppm respectively. The result showed that the maximum concentration of banana peel in adsorbing Rhodamine B was 6 ppm with 120 of contact time, and Rhodamine B adsorption capacity was  4.55mg/g. Keywords: Banana peel, Rhodamine B, biosorbent

IDENTIFIKASI DAN PENETAPAN KADAR RHODAMIN B DALAM KERUPUK BERWARNA MERAH YANG BEREDAR DI PASAR ANTASARI KOTA BANJARMASIN

2017 ◽  
Vol 1 (1) ◽  
pp. 85
Author(s):  
Eka Kumalasari
Keyword(s):  
Thin Layer ◽  
Rhodamine B ◽  
Textile Industry ◽  
Mobile Phase ◽  
Uv Lamp ◽  
Tapioca Flour ◽  
Red Dye ◽  
Layer Chromatography ◽  
Rhodamin B

Crackers are made from tapioca flour batter mixed with flavorings and colorings, still many outstanding crackers that contain ingredients banned dye Rhodamine B. Rhodamine B is a chemical used for red dye in the textile industry and plastic. Rhodamine B can cause cancer, poisoning, lung irritation, sore eyes, and sore throat. This study aims to identify and determination the levels of Rhodamine B in circulating red crackers Antasari market Banjarmasin.The population is that sold in the red crackers that sold in Antasari market Banjarmasin.. The sampling is technique incidental sampling , that is based on chance, so any population by chance met with researchers can be used as a sample. Identification of Rhodamine B was done by Thin Layer Chromatography (TLC) by using the stationary phase silica GF 254 and mobile phase is elution solvent is n-butanol, ethyl acetate, ammonia (10:4:5). Then detected with a UV lamp 254 nm and 366 nm. While for the determination of levels using Vis spectrophotometry at a wavelength of 544 nm.The results showed that the samples of 6 found one sample containing Rhodamine B, namely samples 5 (cassava crackers matches) and obtained values of 7,25 ± 3,8640 levels mg / kg. Based on these results, Rhodamine B still found in crackers that sold in the market Antasari Banjarmasin.

scholarly journals Determination of Delafloxacin in Pharmaceutical Formulations Using a Green RP-HPTLC and NP-HPTLC Methods: A Comparative Study

Antibiotics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 359 ◽  
Author(s):  
Prawez Alam ◽  
Essam Ezzeldin ◽  
Muzaffar Iqbal ◽  
Gamal A.E. Mostafa ◽  
Md. Khalid Anwer ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Silica Gel ◽  
Solid Lipid Nanoparticles ◽  
Ternary Mixture ◽  
Mobile Phase ◽  
Pharmaceutical Formulations ◽  
Lipid Nanoparticles ◽  
Ammonia Solution ◽  
Hptlc Method

In this work; delafloxacin (DLFX) was determined using a validated green RP-HPTLC and NP-HPTLC methods in commercial tablets and in-house developed solid lipid nanoparticles (SLNs). RP-HPTLC determination of DLFX was performed using “RP-18 silica gel 60 F254S HPTLC plates”. However; NP-HPTLC estimation of DLFX was performed using “silica gel 60 F254S HPTLC plates”. For a green RP-HPTLC method; the ternary combination of ethanol:water:ammonia solution (5:4:2 v/v/v) was used as green mobile phase. However; for NP-HPTLC method; the ternary mixture of ethyl acetate: methanol: ammonia solution (5:4:2 v/v/v) was used as normal mobile phase. The analysis of DLFX was conducted in absorbance/reflectance mode of densitometry at λmax = 295 nm for both methods. RP-HPTLC method was found more accurate, precise, robust and sensitive for the analysis of DLFX compared with the NP-HPTLC method. The % assay of DLFX in commercial tablets and in-house developed SLNs was determined as 98.2 and 101.0%, respectively, using the green RP-HPTLC technique, however; the % assay of DLFX in commercial tablets and in-house developed SLNs was found to be 94.4 and 95.0%, respectively, using the NP-HPTLC method. Overall, the green RP-HPTLC method was found superior over the NP-HPTLC. Therefore, the proposed green RP-HPTLC method can be successfully applied for analysis of DLFX in commercial tablets, SLNs and other formulations containing DLFX.

scholarly journals THE ANTIBACTERIAL ACTIVITY OF FRACTIONS OF ETHYL ACETATE GARCINIA LATISSIMA MIQ. STEM BARK EXTRACTS AGAINST BACILLUS SUBTILIS AND PSEUDOMONAS AERUGINOSA

2017 ◽  
Vol 10 (17) ◽  
pp. 69
Author(s):  
Neneng Sitisilfi Ambarwati ◽  
Amarila Malik ◽  
Elfira Amalia Deborah ◽  
Cakra Hagai Arpatism ◽  
Maulfi Hanif ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
Mobile Phase ◽  
Agar Medium ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Tetrazolium Salt ◽  
Inhibitory Zone ◽  
Zone Diameter

 Objective: This research focuses on the antibacterial activities of fractions of ethyl acetate Garcinia latissima Miq. extracts against Bacillus subtilis and Pseudomonas aeruginosa.Methods: For each bacteria, the values of the inhibitory zone diameter were determined using paper discs method, while the minimum inhibitory concentration (MIC) was determined by microdilution and antibacterial assay was determined by bioautography. Fractionation was carried out using gradient elution with silica gel column as stationary phase successively increasing the separation according to the polarity (using combination of eluents; n-hexane, ethyl acetate, and methanol).Results: To determine the diameter of the inhibitory zone for each fraction, the fractions were diluted with dimethyl sulfoxide until the concentration of each fraction was 20,000 ppm. The results showed that fraction B had no inhibitory zone against B. subtilis. The inhibitory zone diameters of fractions A, C, D, E, F, and G against B. subtilis were 7.600±0.000, 6.767±0.202, 7.950±0.477, 7.883±0.901, and 9.233±0.231 mm, respectively. Only Fraction G had an inhibition zone diameter of 7.200±0.173 mm against P. aeruginosa. The active fractions were tested by contact bioautography using silica gel (60 GF254) plate and hexane:chloroform (2:3) as mobile phase for Fractions A, C, D, E, and F and chloroform:ethyl acetate (4:1) as mobile phase for Fraction G. The thin layer chromatography plates were observed under the ultraviolet light at wavelengths of 254 and 366 nm, while the others plate (using in contact bioautography) placing on agar medium that had been inoculated with the bacteria, so the compounds could be diffuse to the medium. The active compound will appeared inhibitory zones in agar medium. The determination of the MIC values using microdilution with methylthiazol tetrazolium salt showed that fractions of the ethyl acetate extracts of G. latissima Miq. stem bark inhibited the growth of B. subtilis more actively than P. aeruginosa. The highest activity of the fractions against B. subtilis was shown by fraction G, which had a MIC value of 78.125 ppm, followed by fractions F (156.25 ppm), E (312.5 ppm), D (625 ppm), C (1250 ppm), B (2500 ppm), and A (5000 ppm). The highest activity of the fractions against P. aeruginosa was Fraction G (5000 ppm), while the MIC values of other fractions were >5000 ppm.Conclusion: Further investigations should be conducted to obtain the new antibacterial compounds in fractions of ethyl acetate extract of G. latissima Miq. stem bark that effective to inhibit the growth of B. subtilis and P. aeruginosa.

scholarly journals Identifikasi dan Penetapan Kadar Rhodamin B pada Eye Shadow secara Kromatografi Lapis Tipis dan Spektrofotometri Ultraviolet-Visible

2020 ◽  
Vol 19 (02) ◽  
pp. 81-86
Author(s):  
Siva Fauziah ◽  
Dede Komarudin ◽  
Citra Dewi
Keyword(s):  
Respiratory Tract ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Rhodamine B ◽  
Uv Light ◽  
Average Value ◽  
Traditional Market ◽  
Layer Chromatography ◽  
Rhodamin B

Abstract Based on BPOM findings in 2016 there is still the presence of Rhodamin B which is used as one of the dyes in cosmetics. It causes irritation to the skin, respiratory tract and is carcinogenic. The purpose of this study to conduct an examination and determination of Rhodamin B in eye shadow in the Kalideres traditional market. The sample used for the research is eye shadow that is bought from cosmetics stores and cosmetic kiosks, which circulated in the Kalideres traditional market. The sample consist of five different brands based on three criteria is eye shadow which does not include the ingredients used, the writing in the packaging used a language other than Indonesia and does not have a permit number from BPOM. This research was conducted to identify the presence of Rhodamin B in eye shadow with KLT method ( thin layer chromatography), the eluent used is N-butanol, etil asetat, amoniac 25% (10 : 4 : 5) then detected with UV light 254 nm and determination of the content using UV-Vis spectrophotometry method at maximum wavelength 553 nm using as blank is methanol. The resulted was presence of Rhodamine B in sample code A and sample code D with the average value of Rhodamine B levels in the sample examined, the sample code A is 1,3063 mg/g and sample code D is 1,2564 mg/g.   Keywords: Rhodamine B, Eye Shadow, KLT, Uv-Vis Spectrophotometry  

scholarly journals PEMBUATAN DAN KARAKTERISASI KATALIS ZnO/KARBON AKTIF DENGAN METODE SOLID STATE DAN UJI AKTIFITAS KATALITIKNYA PADA DEGRADASI RHODAMIN B

2014 ◽  
Vol 7 (2) ◽  
pp. 180
Author(s):  
Upita Septiani ◽  
Ilona Bella ◽  
Syukri
Keyword(s):  
Activated Carbon ◽  
Solid State ◽  
Rhodamine B ◽  
Uv Light ◽  
Composite Catalyst ◽  
X Ray Diffraction ◽  
Sem Images ◽  
Scanning Electronmicroscopy ◽  
Rhodamin B

 ABSTRACTComposite catalyst ZnO/Activated Carbon (ZnO/AC) had been synthesized successfully bysolid state method. Synthesis was done by varying the addition of activated carbon (AC) 2%,5%, and 10% ZnO mass. Composite catalyst were calcinated at 400oC and characterized byFTIR (Fourier Transform Infra Red), XRD (X-Ray Diffraction), SEM (Scanning ElectronMicroscopy). Based on the results of FTIR, absorption appeared in the region wave number1400-1600 cm-1, which indicated stretching of C=C was assumed come from AC. From theXRD results, it was known by the addition of AC, not overly change the crystallinity and crystalsize of ZnO, the crystal structure is hexagonal (wurtzite). SEM images showed AC preventedthe agglomeration of ZnO that would expand the surface area of ZnO and increased catalyticactivity of ZnO. For the results of the catalytic activity test, catalyst ZnO/AC was tested on thedegradation of Rhodamine B solution (10 ppm) by UV light irradiation, where the increasingnumber of trains the catalytic ability of ZnO also increased, it could be concluded that activatedcarbon can support to increase the role of ZnO in degrading Rhodamine B.Keywords: composite catalyst, ZnO, activated carbon, solid state, Rhodamine B

Identifikasi Sediaan Jamu Kuat yang Beredar di Kecamatan Banguntapan dan Pleret Kabupaten Bantul dengan Metode KLT

2019 ◽  
Vol 14 (2) ◽  
pp. 103
Author(s):  
Dewi Umniyatul ◽  
Dwi Kurniawati Sambodo
Keyword(s):  
Herbal Medicine ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Plant Material ◽  
Uv Light ◽  
Sildenafil Citrate ◽  
Quota Sampling ◽  
Layer Chromatography

Background: Traditional herbs are ingredients or herbs in the form of plant material, animal ingredients, mineral ingredients, galenic preparations or mixtures of these ingredients, it has been used as medicine traditionally.Objective: This study is aimed to Identify of Sildenafil Citrate on “Jamu Kuat” (Herbal Medicine) products.Methods: This type of research was descriptive and the sample was determined by quota sampling. The method used Thin Layer Chromatography (TLC) which was a physicochemical method. There were seven samples were extracted by the soxhletation method until a thick extract was obtained to be spotted in the quite phase of TLC silica gel F254. The motion phase used to identify sildenafil citrate was chloroform: ethyl acetate (6: 4). Spots detection was done by observation under UV light 366 nm and the spots that appeared were calculated of Rf value and compared with the comparable Rf value of sildenafil citrate.Results: From this study, there were samples containing sildenafil citrate. Sample C with a sample Rf value of 0.75 and sample D with Rf value of sample 0.78 and a comparable Rf of sildenafil citrate 0.77.Conclusion: The seven samples of “Jamu Kuat” contained sildenafil citrate in Banguntapan and Pleret Districts, Bantul Regency. Keywords: Jamu Kuat (Herbal Medicine), Sildenafil citrate, TLC

scholarly journals Molluscicidal Potential of Column Purified Fractions of Allium cepa against Intermediate Host of Urinary Schistosomiasis (Bulinus globosus) in Sokoto, Nigeria

2018 ◽  
pp. 1-6
Author(s):  
J. Suleiman ◽  
K. Singh ◽  
A. Y. Bala ◽  
M. T. Muhammad ◽  
M. S. Yakubu
Keyword(s):  
Stationary Phase ◽  
Ethyl Acetate ◽  
Intermediate Host ◽  
Laboratory Condition ◽  
Silica Gel ◽  
Allium Cepa ◽  
Mobile Phase ◽  
Intermediate Hosts ◽  
Urinary Schistosomiasis ◽  
Bulinus Globosus

Potential of column purified fractions of Allium cepa bulb against intermediate hosts of urinary schistosomiasis (Bulinus globosus) was conducted in laboratory condition. The fresh bulbs of A. cepa were purchased from Ramin Kura market of Sokoto, identified and authenticated by a taxonomist. The bulbs were sliced into pieces, air dried and powdered. Extracts were obtained using methanol as polar then purified with silica gel as a stationary phase while N-hexane and ethyl acetate (1:1) as the mobile phase. Thirteen fractions each fraction containing 10 ml of the effluent was collected, the collected extracts were left open for evaporation for 48 hours. Ten adult B. globosus were immersed in 3liters of water containing different concentrations of the fraction and each treatment was replicated three times with control in the same condition without treatment, observations were recorded after 24 hours up to 96 hours. The toxicity experiment showed that fractions (F7, F8, F6 and F9) were most toxic fractions, LC50 after 96 hours was 19.371 mg/l. based on findings from this research it can be concluded that, A. Cepa was very potent and can be used for control of B. globosus in order to prevent urinary schistosomiasis infection in endemic areas and drugs industries may use the extracts of these plants for production of molluscicides.

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