scholarly journals Cell proliferation and apoptosis in Wistar rat kidney after renal mass ablation and low-dose irradiation

Medicina ◽  
2010 ◽  
Vol 46 (3) ◽  
pp. 204
Author(s):  
Marina Aunapuu ◽  
Andres Arend ◽  
Mai Ots ◽  
Mara Pilmane
Keyword(s):  
Cell Proliferation ◽  
Wistar Rats ◽  
Low Dose ◽  
Rat Kidney ◽  
Wistar Rat ◽  
Control Group ◽  
Dose Irradiation ◽  
Proliferation And Apoptosis ◽  
Male Wistar Rats ◽  
Distal Tubules

Cell proliferation and apoptosis in the remnant rat kidney after treatment with lowdose irradiation was investigated. Material and methods. In the first group (n=9), adult male Wistar rats underwent 5/6 nephrectomy (NPX); in the second group (n=9), NPX was combined with low-dose irradiation. Rats without surgery and irradiation formed the control group (n=9). Results. Hypertension and proteinuria induced by NPX were decreased by 3-Gy irradiation. The 5/6 NPX rats showed a dramatic increase in proliferating and apoptotic cells in the glomeruli and in the distal tubules at week 2, which was significantly decreased by low-dose irradiation. Conclusion. The data demonstrate that low-dose irradiation is a factor slowing the process of chronic renal injury.

scholarly journals Histologi Lumen Dan Endotelium Arteri Dorsal Penis Tikus Wistar (Rattus Novergicus) Yang Diinduksi Pakan Tinggi Lemak

2020 ◽  
Vol 7 (1) ◽  
pp. 73
Author(s):  
I Wayan Rosiana ◽  
I Gede Widhiantara
Keyword(s):  
Treatment Group ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Wistar Rat ◽  
Lumen Diameter ◽  
Control Group ◽  
High Fat ◽  
Arterial Lumen ◽  
Male Wistar Rats ◽  
The Difference

This study aims to look at the histopathological picture of the dorsal arteries of the penis of the hiperlipidemic wistar rats (Rattus novergicus) induction by high-fat diet that seen in terms of lumen diameter and thickness of the arterial endotelium wall. Hyperlipidemia is a risk factor for ateriosclerosis in the penile arteries causing erectile dysfunction in men. This study is an experimental study with a randomized posttest only control goup design. The sample are  10 individuals adult male wistar rats aged 3-4 months with a range of body weight 150-200 grams. Before treatment, adaptation was carried out for 7 days. After that the sample rats in the treatment group were made hyperlidemic by feeding lard for 50 days. Then surgery is performed for histopathological preparations at the posttest. To determine the differences in endotelium thickness and arterial lumen diameter in the two groups, an independent t-test was used. Thick diameter data of the endotelium and dorsal arteries of the penis of the wistar rat between the lower treatment group and the control group. The difference that occurred was statistically significant (p <0.05). So it can be concluded that the provision of high-fat diet (hyperlipidemia) decreases the lumen diameter and endotelium thickness of dorsal arteries penis. Keywords: Dorsal arteries, high-fat diet, Wistar rats

scholarly journals PENGAMATAN AKTIVITAS TIKUS WISTAR JANTAN DENGAN ALAT OPTO- VARIMEX® PADA KONDISI DIET TINGGI MINYAK TRANS

Jurnal BIOMA ◽  
2015 ◽  
Vol 11 (1) ◽  
pp. 89
Author(s):  
NURUL MASYITA ◽  
KOEKOEH SANTOSO ◽  
NASTITI KUSUMORINI ◽  
ARYANI SISMIN SATYANINGTIJAS ◽  
ATIN SUPIYANI
Keyword(s):  
Adipose Tissue ◽  
Wistar Rats ◽  
Feeding Activity ◽  
Wistar Rat ◽  
Control Group ◽  
Trans Fat ◽  
Treatment Groups ◽  
Track System ◽  
Male Wistar Rats ◽  
Auto Track

ABSTRACT Excessive energi consumption of high-fat diet increase the body’s adipose tissue which also affect the increased secretion of leptin, this is because leptin secretion is proportional to the mass of adipose tissue. Leptin activates the lateral hypothalamus as the center of hunger and feeding activity. The aim of this study was to obtain the relationship between high trans fat feeding on male Wistar rats and feeding activity. This method uses male Wistar rats (Rattus norvegicus) as an animal model. Sixteen rats were grouped into 4 treatment groups. The first group was given additional feed 20% trans fat, the second group was given additional feed 40% trans fat, a third group was given additional feed 60% trans fat, and the last as a control group. This feed was given once a day for 70 days. Observation of activities perform using Opto-varimex® auto-track system ver. 4.31. The results showed an corellation between activity based doses of trans fat. Keywords: Opto-varimex®, Trans fat, Activity, Wistar rat.

scholarly journals Cigarette Smoke Induces Colorectal Carcinogenesis in Wistar Rats by Decreasing The Expression of APC, MSH2 and MLH1

2017 ◽  
Vol 9 (1) ◽  
pp. 66 ◽  
Author(s):  
Dody Novrial ◽  
Ignatius Riwanto ◽  
Sutiman Bambang Sumitro ◽  
Indra Wijaya
Keyword(s):  
Colorectal Cancer ◽  
Cigarette Smoke ◽  
Wistar Rats ◽  
Wistar Rat ◽  
Colorectal Carcinogenesis ◽  
Cigarette Smoke Exposure ◽  
Control Group ◽  
Time Duration ◽  
Epithelial Morphology ◽  
Male Wistar Rats

<p>Colorectal carcinogenesis induced by cigarette smoke requires at least 30-40 years. This long time duration causes an animal research conducted becomes relevant. This research was carried out to observe colorectal carcinogenesis due to cigarette smoke exposure in Wistar Rat. The observations focused on changes in epithelial morphology and expression of APC, MSH2 and MLH1. Twenty male Wistar rats inbreed strain were randomly allocated into control group and experimental group exposure to cigarettes smoke for 14 weeks and 28 weeks sequentially.  Colorectal epithelial morphology was assessed on the histopathology examination, whereas the expression of APC, MSH2 and MLH1 was assessed on aspect of immunohistochemistry. The comparative analysis between the two groups was performed using non-parametric Mann-Whitney U test. Histology of colorectal epithelium showed pattern of colitis associated cancer that was significant both in 14 weeks and 28 weeks of treatment.  This research indicated negative expression of APC, MSH2 and MLH1 in the colorectal cancer that were significant at 28 weeks of exposure. This research implies that chronic exposure to cigarette smoke can induce colitis associated colorectal cancer through decreased expression of APC, MSH2 and MLH1. </p>

scholarly journals Uji efektivitas ekstrak daun sendok (Plantago major L.) terhadap waktu perdarahan pada tikus Wistar jantan (Rattus norvegicus)

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Abedneju R. Kainde ◽  
Damajanty H.C. Pangemanan ◽  
Bernart S.P. Hutagalung
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Bleeding Time ◽  
Wistar Rat ◽  
Control Group ◽  
P Value ◽  
Plantago Major ◽  
Control Group Design ◽  
Group Design ◽  
Male Wistar Rats

Abstract: Herbal plant is often used to treat any kind of disease. There are many kinds of plants in Minahasa used as traditional medicine, inter alia daun sendok (Plantago major L.). Daun sendok is often used to treat bleeding. This study was aimed to obtain the effectiveness of daun sendok extract on bleeding time in male Wistar rats. This was a true experimental study with a posttest only group design. This study was conducted at the Laboratory of Pharmacology Faculty of Medicine University of Sam Ratulangi. Total samples were 14 rats divided into 2 groups: control group and treatment group. Wounds of 2 mm depth were perfomed on the rat tails of the treatment group followed by application of daun sendok extract. The results showed that the average bleeding time of the control group was 51.87 seconds meanwhile of the treatment group was 29.67 seconds. The unpaired t-test between the two groups showed a p value of 0.000. Conclusion: Daun sendok (Plantago major L.) extract could reduce the bleeding time in male Wistar rats. Keywords: bleeding time, daun sendok, Wistar rat Abstrak: Tanaman obat seringkali digunakan untuk mengobati berbagai macam jenis penyakit. Di wilayah Minahasa terdapat banyak jenis tanaman yang digunakan sebagai obat tradisional, salah satunya ialah daun sendok (Plantago major L.) yang seringkali digunakan untuk mengobati perdarahan. Penelitian ini berrtujuan untuk mengetahui efektivitas ekstrak daun sendok terhadap waktu perdarahan tikus Wistar jantan. Jenis penelitian ialah true experimental dengan post test only control group design yang dilakukan di Laboratorium Farmakologi Fakultas Kedokteran Unsrat dengan total sampel berjumlah 14 ekor tikus Wistar yang dibagi menjadi 2 kelompok yaitu: kelompok kontrol dan kelompok perlakuan menggunakan ekstrak daun sendok. Pada kelompok uji diaplikasikan ekstrak daun sendok setelah dilakukan perlukaan di bagian ekor tikus sedalam 2 mm. Hasil penelitian memperlihatkan rerata waktu perdarahan pada kelompok kontrol ialah 51,87 detik sedangkan pada kelompok perlakuan 29,67 detik. hasil uji t tidak berpasangan antara kedua kelompok mendapatkan nilai p=0,000. Simpulan: Ekstrak daun sendok (Plantago major L.) efektif dalam memperpendek waktu perdarahan pada tikus Wistar.Kata kunci: waktu perdarahan, daun sendok, tikus Wistar

scholarly journals EFFECT OF BANANA PEEL FLOUR INFUSION ON SERUM LDL LEVELS OF MALE WISTAR RAT GIVEN HEATED COOKING OIL

2021 ◽  
Vol 1 (2) ◽  
pp. 58-67
Author(s):  
Ilmi Dewi Astuti ◽  
Fuadiyah Nila Kurniasari ◽  
Yosfi Rahmi
Keyword(s):  
Serum Level ◽  
Wistar Rats ◽  
Wistar Rat ◽  
Negative Control ◽  
Male Rats ◽  
Banana Peel ◽  
Control Group ◽  
Soluble Fiber ◽  
Cooking Oil ◽  
Male Wistar Rats

Atherosclerosis is a progressive disease causing a coronary heart disease (CHD). Atherosclerosis is related to the levels of LDL in the blood. An increased LDL in the blood can be prevented by consuming soluble fiber and antioxidants from such Kepok banana peels. The aim of this study is to know the effects of Kepok banana peel flour infusion in various dosage on LDL serum level of male wistar rats which were treated with a repeatedly heated cooking oil. This study used post test control group as experimental design in which 24 male rats were randomly categorized in five groups,  namely (1) a negative control group, (2) a positive control group treated with a repeatedly heated cooking oil and distilled water, as well as the groups treated with a repeatedly heated cooking oil and 0.5, 1, and 2 ml/rats of Kepok banana peel flour solution, for group (3), (4), and (5) respectively. LDL level were measured by using calorimetric method . The result of this study showed that the highest average levels of LDL obtained in control positive group K(+) was 14,25 mg/dl and the lowest average level of LDL obtained in the group treated with a repeatedly heated cooking oil and 0.5 ml/rat  was 11,2 mg/dl. It can be concluded that Kepok banana peel flour can not significantly reduce the mean of LDL serum level of male Wistar rats treated with repeatedly heated cooking oil (p>0,05)

scholarly journals Effects of Lippia multiflora leaf extract on the semen of Wistar rat

2021 ◽  
Vol 39 (2) ◽  
pp. 172-178
Author(s):  
J. O. Olukunle ◽  
E. S. Ajibola ◽  
K. T. Biobaku ◽  
B. S. Okediran ◽  
O. A. Akinkuotu
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Sperm Count ◽  
Experimental Period ◽  
Wistar Rat ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Lippia Multiflora ◽  
Male Wistar Rats ◽  
Dose Dependent

To investigate the effect of Lippia multiflora (Verbanaceae) on the reproductive activity of male Wistar rats, three groups (A,B and C) of rats were treated with 400, 800 and 1600 mg/kg/day of Lippia multiflora leaves extract respectively for 28 days while 5ml/kg of distilled water was given to the control group D. At the end of the experimental period, animals were sacrificed and sperm characteristics, histology of the testes and epididymis were assessed. Lippia multiflora leaf extract caused a significant reduction (P<0.05) in sperm motility, a statistically significant dose dependent reduction in the sperm count and a significant (P<0.05) dose dependent increase in morphological abnormalities of the spermatozoa of the treated rats. Histopathological evaluation of the testis and epididymis revealed varying degrees of degeneration and necrosis of the germinal epithelia cell of the seminiferous tubules, spermiostasis and complete epididymal aspermia. Lippia multiflora leaf extract has significant anti-spermatogenic effects on adult male Wistar rats which could impair reproductive activities in these rats

scholarly journals Synergetic Treatment of Ascorbic Acid and Nicotine Ameliorates Aluminium Induced Neurotoxicity in the Prefrontal Cortex of Wistar Rat

2021 ◽  
Vol 5 (2) ◽  
pp. 282-288
Author(s):  
Taiwo A. Abayomi
Keyword(s):  
Ascorbic Acid ◽  
Body Weight ◽  
Prefrontal Cortex ◽  
Wistar Rats ◽  
Amyloid Plaques ◽  
Wistar Rat ◽  
Aluminium Chloride ◽  
Control Group ◽  
Male Wistar Rats

Background: Though the neuroprotective roles of ascorbic acid are well established, the therapeutic role of nicotine in various neurological disorders is attracting increasing attention. This study evaluated the putative ameliorative role of the synergetic treatment of nicotine and ascorbic acid against neurodegenerative consequences associated with free radical species and amyloid plaques generation in adult male Wistar rats Methods: A total of 35 Wistar rats were distributed into five groups labeled A-E. Group A served as the control group; animals in group B were treated with 100mg/kg body weight of aluminium chloride (AlCl3) for 21 days. Group C animals were treated with 100mg/kg body weight of aluminium chloride for 21 days and post-treated with 14mg/kg body weight of nicotine for 21 days. Group D was treated with 100mg/kg body weight of aluminium chloride for 21 days and post-treated with 100mg/kg bodyweight of ascorbic acid for 21 days. Group E animals were treated with 100mg/kg body weight of aluminium chloride for 21 days and post-treated with 100mg/kg bodyweight of ascorbic acid and 14mg/kg body weight of nicotine. On completion of treatments, the prefrontal cortex was excised and processed for biochemical and histochemical examinations. Results: Oxidative stress was evident from the diminished level of catalase and glutathione per oxidase and elevated lipid peroxidation levels in animals administered with aluminium in addition to the presence of amyloid plaques in these animals. However, synergetic administration of ascorbic acid and nicotine attenuated these oxidative and histochemical perturbations induced by aluminium. Conclusion: Synergetic treatment with ascorbic acid and nicotine provided better ameliorative potential against aluminium-induced neurotoxicity compared to either ascorbic acid or nicotine treatments alone

scholarly journals The Effect of Pegagan (Centella Asiatica (L.) Urban) Ethanol Extracts on Hippocampal PSD-95 Protein Expression in Male Wistar Rats

2018 ◽  
Vol 23 (3) ◽  
pp. 85
Author(s):  
Adibah Ferhad ◽  
Auliyani Andam Suri ◽  
Astri Handayani ◽  
Sri Redjeki ◽  
Ria Kodariah
Keyword(s):  
Protein Expression ◽  
Wistar Rats ◽  
Memory Function ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Wistar Rat ◽  
Immunohistochemical Method ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats

One effort to overcome the decline in memory function is through herbal medicine. Pegagan (Centella asiatica (L.) Urban) contain the active components of triterpenoid and flavonoids, has been known to be able to improve memory function. Synaptic plasticity is the basis of memory formation which is strongly influenced by synapse proteins such as PSD-95. Loss of PSD-95 protein can cause memory function decline. This study aims to determine the effect of 70% ethanol extract of pegagan toward PSD-95 protein expression on hippocampus of male Wistar rat. Eighteen male Wistar rats were randomly divided into 3 groups, 6 rats/group: group (1) given ethanol extract of pegagan with dose 300 mg / kgBW / day (CA300), (2) given ethanol extract of pegagan with dose 600 mg / kgBW / day (CA600), and (3) control group (K), given daily aquadest. All three groups were treated for 28 consecutive days. At the end of the treatment period, rats were decapitated and the hippocampus was isolated from the brain. Analysis of protein was done by immunohistochemical method. Statistical analysis was performed by One Way ANOVA parametric test followed by Post-Hoc Bonferroni. The results showed that there was no significant difference between group K and group CA300 (P = 0.123), whereas there were significant differences between CA600 group and K group and CA300 group (P = 0.000). From this research, it can be concluded that 70% ethanol extract of pegagan with dose 600mg / kgBW / day can increase expression of PSD-95 protein on hippocampus of male Wistar rat.

THE CHEMOTHERAPEUTIC ROLE OF p-MCA IN AVERTING NDEA INDUCED HEPATIC CARCINOGENESIS IN EXPERIMENTAL WISTAR RATS

2021 ◽  
pp. 53-59
Author(s):  
Sriragavi Ravi ◽  
N. Nalini
Keyword(s):  
Oxidative Stress ◽  
Hepatocellular Carcinoma ◽  
Cell Proliferation ◽  
Wistar Rats ◽  
Morphological Changes ◽  
Primary Liver Cancer ◽  
Experimental Period ◽  
Control Group ◽  
Male Wistar Rats ◽  
Group 3

OBJECTIVES: Hepatocellular carcinoma (HCC) is the primary liver cancer and second leading cause of cancer related deaths worldwide. The aim of this present study was to evaluate the biochemical, histopathological and chemotherapeutic efcacy of p-methoxycinnamic acid (p-MCA) against N- nitrosodiethylamine (NDEA) in a rat model of hepatocellular carcinoma. MATERIALS AND METHODS: Approximately thirty male wistar rats weighing 150-200 g were designated for this study. The rats were arbitrarily separated into ve groups and each group comprised of six rats. Group 1 served as control; Group 2 rats received p-MCA at the dose of 80 mg/kg b.w. Group 3, 4 and 5 rats were induced HCC using NDEA. 2-acetylaminouorene (AAF) was used as a promotor. Group 4 and 5 rats received p-MCA at the doses of 40 and 80 mg/kg b.w. throughout the 12 week experimental period. At the end of the experimental period, liver tissues from all the rats were collected and liver specic enzymes, lipid peroxidation, markers, xenobiotic metabolizing enzymes, antioxidant status and brotic markers were evaluated.RESULTS: NDEA administration induced hepatocyte damage, oxidative stress, cell proliferation, inammation and brosis. The liver sections from NDEA induced group 3 rats showed loss of lobular architecture, morphological changes in the nuclei and DNA damage. Administration of p-MCA to NDEA treated rats restored the hepatic architecture, enzyme activities, cell proliferation, inammation and brosis.CONCLUSION: We conclude that oral administration of p-MCA for 12 weeks exerts a signicant therapeutic effect against HCC by regulating the concentration of specic hepatic and xenobiotic enzymes, suppressing oxidative stress, inhibiting cell proliferation and reducing the inammatory response.

Pengaruh Profilaksis Ekstrak Ubi Jalar Ungu (Ipomoea batatas L.) terhadap Kadar Malondialdehida Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Wistar yang Diinduksi Karagenan

2018 ◽  
Vol 15 (2) ◽  
pp. 146
Author(s):  
BRILIAN DINANTI ◽  
FITRI HANDAJANI
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Wistar Rats ◽  
Group Method ◽  
Control Group ◽  
Intraplantar Injection ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Potato Extract ◽  
Purple Sweet Potato

<p>Liver is an organ with complex metabolism. When the liver is inflamed, cellular immunity will defend against inflammatory agents by stimulating immune cells to produce reactive oxygen species (ROS). Excessive ROS accumulation cause oxydative stress with increased  liver malondialdehyde (MDA) level. Some researches showed that purple sweet potato contain flavonoids (anthocyanins) that functioned as antioxydants. This study aimed to show the prophylactic effect of purple sweet potato extract to the liver MDA level of male Wistar rats induced by carrageenan.</p><p>This study used post-only control group method using 18 male Wistar rats divided into 3 groups: group of rats without treatment, group of rats induced by 0,1 ml of 1% carrageenan by intraplantar injection on day-8, and group of rats given with 872 mg/kgBW of purple sweet potato extract for 7 days and induced by 0,1 ml of 1% carrageenan. In the end of the study, the liver MDA levels were measured by Thio-Barbituric Acid method on each groups.</p><p>The results of One-Way ANOVA test showed there was no significant difference (p = 0,290) between group of rats without treatment (<em>x̅</em>= 207,50) and group of rats induced by carrageenan (<em>x̅</em>=233,17). Then, there is no significant difference (p = 0.978) between group of rats induced by carrageenan and group of rats given with prophylactic purple sweet potato extract and induced by carrageenan (<em>x̅</em>= 232,50).</p><p>The conclusion of this study is giving intraplantar injection of carrageenan can increase liver MDA level insignificantly and giving prophylactic purple sweet potato extract has an effect to decrease the liver MDA level of rats induced by carragenan insignificantly because it contains anthocyanins as antioxidants.</p><p> </p><strong>Keywords: </strong>Liver, <em>Ipomoea batatas</em> L., Malondialdehyde, Anthocyanins

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