Gıda Patojenlerine Karşı Cardopatium corymbosum L.’ nin Biyolojik Aktivitelerinin Belirlenmesi

Food spoilage pathogens threaten to human health. In this work was studied biological activity of plant known as the “kurtludiken” in Turkey and it is contributed to the literature. Cardopatium corymbosum L. from Mugla region were collected for antimicrobial and antioxidant activity studies. The antimicrobial activity studies were done by disc diffusion method. A total of 8 microorganisms were used at this study, one of them is yeast and the others are bacteria. The 2,2′-azinobis-(3 ethyl benzothiazoline-6-sulfonic acid) (ABTS•+) was used for non-enzymatic antioxidant activity experiments. There are few studies on the biological activities of Cardopatium corymbosum. The aim of this study is to determine the antimicrobial and antioxidant effects of Cardopatium corymbosum against food pathogens. The radical scavenging percentage of plant was found to be 13%. As a result, Cardopatium corymbosum have antimicrobial and antioxidant capacities.

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The Citrus reticulata essential oil: evaluation of antifungal activity against penicillium species related to bakery products spoilage

Potravinarstvo Slovak Journal of Food Sciences ◽

10.5219/1695 ◽

2021 ◽

Vol 15 ◽

pp. 1112-1119

Author(s):

Hana Ďúranová ◽

Veronika Valková ◽

Lucia Galovičová ◽

Jana Štefániková ◽

Miroslava Kačániová

Keyword(s):

Antioxidant Activity ◽

Antifungal Activity ◽

Essential Oil ◽

Vapor Phase ◽

Radical Scavenging ◽

Diffusion Method ◽

Wheat Bread ◽

Citrus Reticulata ◽

Disc Diffusion ◽

Disc Diffusion Method

Fungal food spoilage plays a key role in the deterioration of food products, and finding a suitable natural preservative can solve this problem. Therefore, antifungal activity of green mandarin (Citrus reticulata) essential oil (GMEO) in the vapor phase against the growth of Penicillium (P.) expansum and P. chrysogenum inoculated on wheat bread (in situ experiment) was investigated in the current research. The volatile compounds of the GMEO were analyzed by a gas chromatograph coupled to a mass spectrometer (GC–MS), and its antioxidant activity was determined by testing free radical-scavenging capacity (DPPH assay). Moreover, the disc diffusion method was used to analyze the antifungal activity of GMEO in in vitro conditions. The results demonstrate that the Citrus reticulata EO consisted of α-limonene as the most abundant component (71.5%), followed by γ-terpinene (13.9%), and β-pinene (3.5%), and it displayed the weak antioxidant activity with the value of inhibition 5.6 ±0.7%, which corresponds to 103.0 ±6.4 µg TEAC.mL-1. The findings from the GMEO antifungal activity determination revealed that values for the inhibition zone with disc diffusion method ranged from 0.00 ±0.00 (no antifungal effectiveness) to 5.67 ±0.58 mm (moderate antifungal activity). Finally, exposure of Penicillium strains growing on bread to GMEO in vapor phase led to the finding that 250 μL.L-1 of GMEO exhibited the lowest value for mycelial growth inhibition (MGI) of P. expansum (-51.37 ±3.01%) whose negative value reflects even supportive effect of the EO on the microscopic fungus growth. On the other hand, GMEO at this concentration (250 μL.L-1) resulted in the strongest inhibitory action (MGI: 54.15 ±1.15%) against growth of P. chrysogenum. Based on the findings it can be concluded that GMEO in the vapor phase is not an effective antifungal agent against the growth of P. expansum inoculated on bread; however, its antifungal potential manifested against P. chrysogenum suggests GMEO to be an appropriate alternative to the use of chemical inhibitors for bread preservation.

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ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF Tinospora crispa (putarwali) STEMS METHANOLIC EXTRACT

Jurnal Teknologi ◽

10.11113/jt.v78.9941 ◽

2016 ◽

Vol 78 (11-2) ◽

Cited By ~ 1

Author(s):

Fatimatul Akmal Sulaiman ◽

Nurfarahin Fuad ◽

Farawahida Rahman ◽

Anwar Iqbal ◽

Deny Susanti Darnis

Keyword(s):

Antimicrobial Agents ◽

Methanolic Extract ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Antimicrobial Properties ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Average Percentage ◽

Dried Extract

Apart from being the primary source of food to other living things, plants also have medicinal value to treat various kinds of diseases. In recent years, it has been proposed that the extract from plants may be used as natural antioxidants which can help to prevent the generation of carcinogens in human body. In addition, plants also have antimicrobial agents to inhibit the growth of pathogenic microbes. This study was intended to investigate the antioxidant properties and antimicrobial activity of methanolic extract of Tinospora crispa stems extracted using soxhlet extraction method. The antimicrobial properties of T. crispa stems extract were tested using disc diffusion method against Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Candida albicans IMR C S23/11 A and Saccharomyces cerevisiae IMR S 617/068. The antioxidant properties of the extract were investigated by using Total Phenolics Content (TPC), Total Flavonoids Content (TFC), DPPH free radical scavenging and b–carotene bleaching assays. The TPC value was 6.12 g GAE/100 g of dried extract while the TFC value was 55.58 g QE/100 g of dried extract. The IC50 of DPPH scavenging assay for the extract and ascorbic acid were 0.21 and 0.04 mg/mL, respectively. The average percentage of b–carotene bleaching assay was 38.3 % as compared to BHT, which was 45.1%. The disc diffusion method showed no inhibition zone against all the strains of microorganisms at all concentrations of the extracts (0.5, 1.0, 2.5 and 5.0 mg/disc).

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ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF RUTA ANGUSTIFOLIA EXTRACT

Jurnal Teknologi ◽

10.11113/jt.v77.6747 ◽

2015 ◽

Vol 77 (25) ◽

Cited By ~ 1

Author(s):

Nur Azfa Shuib ◽

Anwar Iqbal ◽

Fatimatul Akmal Sulaiman ◽

Izzatie Razak ◽

Deny Susanti

Keyword(s):

Antibacterial Activity ◽

Phenolic Content ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Diffusion Method ◽

Total Phenolic ◽

Disc Diffusion ◽

Flavonoid Content ◽

Disc Diffusion Method ◽

Polyphenolic Content

Ruta angustifolia was used in this study in order to evaluate the antimicrobial activity and antioxidant properties and its correlation with the polyphenolic content. Two Gram-positive bacteria (Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778) and two Gram–negative bacteria (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 8739) were used to determine the antibacterial activity. Aqueous maceration extract was used for antioxidant activities and methanolic maceration extract was used for antibacterial activity. The antioxidant properties and activities were evaluated by using total phenolic content (TPC), total flavonoid content (TFC), DPPH free radical scavenging activity and beta-carotene bleaching method. Whereas, the antibacterial activity was examined using disc diffusion method against selected microorganism at concentration 1.0 mg/disc. The results showed the phenolic content of R. angustifolia extract was 18.89 g GAE/100 g extract while the flavonoid content was 14.170 g QE/100 g extract. R. angustifolia exhibited good radical scavenging with IC50 value of 2.04 mg/ml. The result for disc diffusion method showed no inhibition zone against all the strains of bacteria at 1.0 mg/disc concentration of the extract. Based on the results, it can be concluded that the R. angustifolia aqueous extract has the antioxidant properties and there is correlation between polyphenolic content of the extract with its antioxidant activity. However, R. angustifolia methanolic extract did not show any antibacterial activity.

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EVALUATION OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF ISOLATED CONSTITUENTS FROM AREAL PART OF CUSCUTA REFLEXA ROXB. PLANT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i3.22475 ◽

2018 ◽

Vol 11 (3) ◽

pp. 187

Author(s):

Sandeep Sandeep ◽

Abhilasha Mittal ◽

Abhilasha Mittal

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Diffusion Method ◽

E Coli ◽

Cuscuta Reflexa ◽

Isolated Compound ◽

Antimicrobial And Antioxidant Activity ◽

Dpph Radical Scavenging

Objective: The objective of the study was to investigate the pharmacological evaluation of previously isolated compounds (CR-1 to CR-5) from the areal part of Cuscuta reflexa Roxb. is reported.Methods: The antimicrobial and antioxidant activity of the isolated compounds (CR-1 to CR-5) from C. reflexa was determined by the disc-diffusion method and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) model, respectively. The antimicrobial activity was performed against four strains Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa.Results: The results revealed that highest zone of inhibition is measured by compound CR-5 against E. coli. The antioxidant activity is evaluated for in vitro antioxidant activity using DPPH radical scavenging activity, inhibitory concentration 50% (IC50) (120.92–76.38 %), respectively. The results indicate that isolated compound CR-1 and CR-2 having IC50 76.38 and 76.94 μg/ml, respectively, showed potent antioxidant activity comparable to standard ascorbic acid (IC50 43.42 μg/ml).Conclusion: This study suggests that areal part of C. reflexa have bioactive compounds for a new antimicrobial and antioxidant drug development.

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ISOLASI JAMUR SIMBION ASCIDIA Schizophyllum commune YANG MEMILIKI AKTIVITAS ANTIBAKTERI

JURNAL PESISIR DAN LAUT TROPIS ◽

10.35800/jplt.9.1.2021.33569 ◽

2021 ◽

Vol 9 (1) ◽

pp. 22

Author(s):

Gianfranco Montolalu ◽

Deiske A Sumilat ◽

Natalie D.C. Rumampuk ◽

Inneke FM Rumengan ◽

Rosita AJ Lintang ◽

...

Keyword(s):

Biological Activities ◽

Schizophyllum Commune ◽

Inhibition Zone ◽

Benthic Invertebrate ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Self Defense ◽

Identification Of Fungi ◽

Symbiotic Fungi

Ascidian is a benthic invertebrate that produces secondary metabolites. The substances were produced by the ascidian as self-defense from many environmental factors. Several studies report the similar structure between the substances extracted from ascidian and symbiotic fungi with biological activities. This study aims to isolate fungi associated with ascidian Eudistoma sp., then observe its potency on inhibiting bacteria S. aureus dan A. hydrophila. PDA was used to isolate and culture fungal isolate, then extraction using solvent ethyl acetate. Antibacterial activity of this symbiotic fungi isolates extract was performed using the Kirby-Bauer disc diffusion method. Molecular identification of fungi results that the symbiotic fungi were Schizophyllum commune. The observing result showed that fungal extract has the ability on inhibiting the growth of S. aureus and with the inhibition zone 8 mm and A. hydrophila 8,5 mm.Keywords: Ascidian, Eudistoma sp., Schizophyllum commune, Symbiotic, Antibacterial

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DETERMINATION OF BIOLOGICAL ACTIVITIES OF THREE MARINE ALGAE COLLECTED FROM VISAKHAPATNAM COAST

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i12.20857 ◽

2017 ◽

Vol 10 (12) ◽

pp. 274 ◽

Cited By ~ 2

Author(s):

Satya Lakshmi S

Keyword(s):

Antioxidant Activity ◽

Marine Algae ◽

Biological Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Inflammatory Activity ◽

Diffusion Method ◽

Dpph Method ◽

Anti Inflammatory

Objective: Determination of biological activities of marine algae collected from the Visakhapatnam coastal region.Methods: Antibacterial activity of algal extracts determined by the well diffusion method, antioxidant activity was determined by reducing power (RP) method and 1, 1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging method finally anti-inflammatory activity was determined by human red blood cell stabilization method and egg albumin method.Results: Methanolic extracts of three algae have potential inhibition activity against Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus. Green alga Enteromorpha compressa has better antioxidant activity compared to the Gracilaria arcuata and Ulva fasciata when tested in RP and DPPH method. U. fasciata found good anti-inflammatory activity among the selected three algae.Conclusion: The three tested algae exhibited significant antibacterial and antioxidant activity compared to anti-inflammatory activity. These bioactive compounds containing macroalgae may find their commercial potential in medicine, food, and cosmetic industry

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THE FUNCTIONAL CHARACTER OF Auricularia auricula CRUDE POLYSACCHARIDES: ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY

BERITA BIOLOGI ◽

10.14203/beritabiologi.v19i3b.3988 ◽

2020 ◽

Vol 19 (3B) ◽

Author(s):

Rizki Rabeca Elfirta ◽

Iwan Saskiawan

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Functional Food ◽

Hot Water ◽

Diffusion Method ◽

Disc Diffusion ◽

Clear Zone ◽

Disc Diffusion Method ◽

Auricularia Auricula ◽

Potential Source

The food stuffs can be clasified as functional food since the foods can improve the human health. One of them are the food stuffs which have function as antioxidant and antibacterial. These activities were studied on crude polysaccharides from Auricularia auricula. The sample was subsequently isolated using hot water and 1 M NaOH to obtain water and alkali soluble of crude polysaccharides. The antioxidant activity was evaluated using β-caroten-linoleat assay. The results showed that the alkali soluble of crude polysaccharides had the highest antioxidant activity (85.82%) at 350 μg/ml. The water and alkali soluble of crude polysaccharides from A.auricula were evaluated for their antibacterial activity using disc diffusion method. The alkali soluble of crude polysaccharides was found to have the highest antibacterial activity at 100 mg/ml against Staphylococcus aureus InaCC B4 and Escherichia coli InaCC B5 with clear zone values of 3.18 mm and 5.10 mm, respectively. The findings indicated that the alkali soluble of crude polysaccharides from A. auricula could potentially be used in part of well-balanced diets and could be considered as a potential source of natural antioxidant and antibacterial products.

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Chemical Composition and Biological Activities of Eugenia caryophyllata Thunb Essential Oil

Asian Journal of Applied Chemistry Research ◽

10.9734/ajacr/2021/v9i430224 ◽

2021 ◽

pp. 51-62

Author(s):

Fatiha Abdellah ◽

Badra Boubakeur ◽

Noura Ayad ◽

Laid Boukraa ◽

Si Mohamed Hammoudi ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oil ◽

Pathogenic Bacteria ◽

Biological Activities ◽

Radical Scavenging ◽

Antioxidant Effect ◽

Diffusion Method ◽

Antioxidant Power ◽

Mic Value

Aims: In this study the antibacterial and antioxidant activities of the essential oil of Eugenia caryophyllata were investigated. Study design: The study contains determination of the chemical composition of the essential oil of E. caryophyllata and the in vitro evaluation of the antibacterial and antioxidant activity of this oil. Place and Duration of Study: The study was carried out at the laboratory of research on local animal products of Ibn-Khaldoun University, Tiaret, Algeria during the period from December 2020 to March 2021. Methodology: The essential oil composition was characterized by gas chromatography/mass spectrophotometrical analyses. The antibacterial activity of this oil was tested against four pathogenic bacteria: Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 33862, Bacillus cereus ATCC 11778 by using disc diffusion method and agar incorporation method to determine the Minimum Inhibitory Concentration (MIC) of the tested oil. The antioxidant activity was evaluated by using DPPH radical scavenging, hydrogen peroxide scavenging assays and ferric reducing antioxidant power (FRAP) assay. Results: Our results have shown a greater antibacterial effect of E. caryophyllata essential oil against all the bacterial tested strains with inhibition zone diameters varied from 17.5 to 20.5 mm and minimal inhibition concentration (MIC) ranged between 0.8 μl / ml and 4.4 μl / ml, B. cereus and S. aureus are the most sensitive species with a MIC value of 0.8 μl / ml, however P. aeruginosa is the most resistant species with a MIC value of 4.4 μl / ml. The result of the antioxidant effect showed that the essential oil of E. caryophyllata is a powerful antioxidant that expresses a higher antioxidant activity than the standard antioxidants: gallic acid, vitamin C and BHT. Conclusion: The obtained results suggest that the essential oil of E. caryophyllata has a strong antibacterial and antioxidant effect and it may be an alternative natural source medicine to prevent and treat many diseases caused by pathogenic bacteria and oxidative stress.

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Antioxidant and antibacterial activity of Acorus calamus. L leaf and rhizome extracts

Jurnal Gizi Klinik Indonesia ◽

10.22146/ijcn.17937 ◽

2017 ◽

Vol 13 (4) ◽

pp. 144 ◽

Cited By ~ 1

Author(s):

Kho See Li ◽

Chan Sook Wah

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Biological Activities ◽

Radical Scavenging ◽

Diffusion Method ◽

Total Phenolic ◽

Acorus Calamus ◽

Methanol Extracts ◽

Antioxidant Agents ◽

Sweet Flag

Background: Acorus calamus (sweet flag) is a well-known traditional herb used in India. There were only limited studies done on both hydrophilic and hydrophobic extracts of A. calamus and the biological activities of phenolic and flavonoid compounds as well as alpha-asarone in the sweet flag.Objective: The present study was designed to explore the antioxidant and antibacterial activities of both hydrophilic and hydrophobic of A. calamus leaf and rhizome extracts. Lastly, the correlation between the biological activities and total phenolic content (TPC), total flavonoid content (TFC) as well as total alpha-asarone content (TAC) of three different extracts (water, hexane and methanol extracts) was revealed.Method: TAC was determined through using HPLC method, while TPC and TFC were determined through spectrophotometric method. DPPH-free radical scavenging assay (DPPH-FRS) and ferric reducing power assay (FRAP) were used to analyze antioxidant activity, whereby disc diffusion method was used to analyze the antibacterial activity.Results: Methanol extracts had significantly (p<0.05) highest levels of antioxidant activities, antibacterial activity TPC (4.466%1; 3.603%2) and TFC (12.819%1; 7.411%2) content. The significant (p<0.05) highest TAC was observed in hexane extracts (0.762%1; 2.975%2). TPC (p<0.05; r=0.855) and TFC (p<0.05; r=0.855) correlated to FRAP, while DPPH-FRS solely correlated to TFC (p<0.05; r=0.873).Conclusion: Both of the hydrophilic and hydrophobic extracts of A. calamus could be potent antioxidant agents for nutraceutical application and antibacterial agent for food application. Lastly, flavonoids and phenols extracted from sweet flag could be a good potent source of antioxidants for nutraceuticals or food additives as those compounds are correlated to the antioxidant activity.1 Leaf extract; 2Rhizome extract

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Determination of Antimicrobial and Antioxidant Activity of Alchemilla alpina L.

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v9i12.2260-2264.4571 ◽

2021 ◽

Vol 9 (12) ◽

pp. 2260-2264

Author(s):

Şule İnci ◽

Ayşe Eren ◽

Sevda Kirbağ ◽

Ahmet İsmail Özkan

Keyword(s):

Antioxidant Activity ◽

Oral Care ◽

Folk Medicine ◽

Radical Scavenging ◽

Antimicrobial Effect ◽

Diffusion Method ◽

Dpph Radical ◽

The People ◽

Scavenging Capacity ◽

Antimicrobial And Antioxidant Activity

Alchemilla genus, which belongs to the Rosaceae family, is a medicinal plant used for various purposes among the people. Species of this genus are known in Turkish folk medicine as lion claw or hazelnut grass. Especially, they are used mainly women’s illnesses, in gastritis, anti-inflammatory, as carminative, and in the treatment of wound. Besides the antimutagenic effect of Alchemilla alpina L., its above-ground parts are used for antimycotic purposes in the form of tea or oral care water. In this study, it has been aimed to determine the antimicrobial effect of the above-ground parts of Alchemilla alpina extracts obtained from methanol, ethanol and chloroform and the antioxidant activity of different concentrations of the extract obtained from methanol. The antimicrobial activity of methanol, ethanol and chloroform extracts of the above-ground parts of A. alpina has been determined according to disk disc diffusion method. In the results obtained have been showed that these extracts inhibited the growth of some bacteria (Staphylococcus aureus ATCC25923, Escherichia coli ATCC25322, Klebsiella pneumoniae ATCC700603, Bacillus megaterium DSM32) and yeasts (Candida albicans FMC17 and Candida glabrata ATCC66032) at different rates (8-23 mm). The antioxidant activity of different concentrations (1.25, 2.5, 5 and 10 mg/ml) of the above-ground parts of A. alpina extract obtained from methanol has been determined according to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity method. In the results obtained, it has been observed that the effect of removing DPPH radical of A. alpina increased depending on increasing concentrations.

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