scholarly journals The antioxidant activity of extracts of the mycelium and the culture fluid of medicinal macromycetes of Pholiota (Fr.) P. Kumm. genus

2021 ◽  
Vol 19 (4(76)) ◽  
pp. 47-53
Author(s):  
Liubov V. Regeda ◽  
Nina A. Bisko ◽  
Nina V. Gurinovych
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Culture Fluid ◽  
Fungal Species ◽  
Culture Collection ◽  
National Academy Of Sciences ◽  
Surface Method ◽  
Mushroom Culture ◽  
Academy Of Sciences ◽  
First Time

Aim. To determine the value of the antioxidant activity of the biomass and culture fluid extracts of strains of seven species of Pholiota genus: P. adiposa, P. alnicola, P. aurivella, P. limonella, P. nameko, P. squarrosa, P. subochracea, which stored in the Mushroom Culture Collection (IBK) of the M. G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine.Materials and methods. The antioxidant properties of the biomass and culture fluid extracts of strains of Pholiota genus were determined by the method of Elfahri et al. using DPPH (1,1-diphenyl-2-picrylhydrazyl). Mycelia of the strains studied were grown by the surface method on a liquid glucose-pepton-yeast medium. The culture fluid was separated from the mycelial biomass by filtration through a capron filter. The absorption of methanol extracts of the culture fluid and the biomass of the strains studied was measured at 517 nm on a SF 46 LOMO spectrophotometer.Results and discussion. Comparing the data obtained we can conclude that the antioxidant effect is significantly higher in the case of methanol biomass extracts – the indicators ranged from 65.98 ± 0.98 % (P. nameko) to 83.6 ± 1.4 % (P. alnicola). As for the culture fluid extracts, the maximum values were recorded in the case of P. limonella (38.3 ± 1.14 %), and the minimum values were observed for P. subochracea (7.37 ± 0.46 %).Conclusions. For the first time, the value and limits of variation in the antioxidant activity of the biomass (65-83 %) and culture fluid extracts (7.4-38 %) have been determined for strains of medicinal fungal species P. adiposa, P. alnicola, P. aurivella, P. limonella, P. nameko, P. squarrosa, P. subochracea.

scholarly journals The contents of ganoderic acids in mycellium of different Ganoderma species (Ganodermataceae) obtained by different methods of cultivation

2021 ◽  
Vol 84 (1) ◽  
pp. 14-18
Author(s):  
D. Boromenskyi ◽  
N. Bisko ◽  
G. Al-Maali ◽  
O. Polishchuk
Keyword(s):  
Submerged Culture ◽  
Culture Collection ◽  
Extraction Time ◽  
Submerged Cultivation ◽  
National Academy Of Sciences ◽  
Cultivation Method ◽  
Ganoderic Acids ◽  
Mushroom Culture ◽  
Ganoderma Sinense ◽  
Academy Of Sciences

The effect of different cultivation methods on the content of ganoderic acids of 7 species, 10 strains of the Ganoderma genus (Ganoderma applanatum 1899; Ganoderma сarnosum 2502; Ganoderma lucidum 1904; Ganoderma resinaceum 2477, 2503; Ganoderma sinense 2516; Ganoderma tsugae 1848, 2024, 2566, Ganoderma oregonense 2560) genus fungi from the IBK Mushroom Culture Collection M.G. Kholodny Institute of Botany of National Academy of Sciences of Ukraine was investigated. It has been shown that the submerged cultivation method is more efficient for the accumulation of ganoderic acids for five strains. In the mycelium of the strain G. sinense 2516 was the highest content of ganoderic acids – 25.2 ± 1.5 mg / g. The productivity (yeld) of ganoderic acids synthesis is much higher with using the submerged culture cultivation method for mycelium of all used species and strains since the use of this method provides the accumulation of much more biomass in comparison with the static liquid cultivation method. The highest yield amount of ganoderic acids was in the mycelium of the G. tsugae 2024 and G. tsugae 2566 species, namely: 0.35 ± 0.019 and 0.36 ± 0.028 g / l. It was proved that the modified extraction method significantly reduces the extraction time of ganoderic acids. Extraction time is reduced from 14 to 2 days. For the G. sinense 2516 and G. tsugae 2024 strains was determined content of the ganoderic acids and their yield in dynamics of grows in the submerged culture on 6, 8, 10, 12, 14, 16, 18 and 20 day of cultivation. The highest amount of the ganoderic acids content was accumulated by the mycelium of the strain G. sinense 2516 – it was 26.4 ± 1.5 mg / g on the 14th day of cultivation. The highest yield of the ganoderic acids was in G. sinense 2516 on 14th day, and G. tsugae 2024 mycelium on the 16th day of cultivation with the next numbers 0.6 ± 0.031, 0.62 ± 0.033 and 0.62 ± 0.027 g/l.

Flavan hetero-dimers in the Cymbopogon citratus infusion tannin fraction and their contribution to the antioxidant activity

2015 ◽  
Vol 6 (3) ◽  
pp. 932-937 ◽  
Author(s):  
Gustavo Costa ◽  
Susana González-Manzano ◽  
Ana González-Paramás ◽  
Isabel Vitória Figueiredo ◽  
Celestino Santos-Buelga ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Cymbopogon Citratus ◽  
First Time

For the first time, flavan heterodimers were identified in Cymbopogon citratus infusion. These compounds seem to be the main contributors to the overall antioxidant properties of the lemongrass infusion.

scholarly journals Iron and Steel Institute in the system of the National Academy of Sciences of Ukraine. Experience and prospects.

2018 ◽  
pp. 3-17
Author(s):  
A.I. Babachenko ◽  
A.S. Vergun ◽  
L.G. Tuboltsev
Keyword(s):  
National Academy Of Sciences ◽  
Iron And Steel ◽  
Strategic Direction ◽  
History Of ◽  
Rolling Production ◽  
The Creation ◽  
Basic And Applied Research ◽  
Academy Of Sciences ◽  
First Time ◽  
Steel Institute

The history of the creation of the Iron and Steel Institute is given. It is shown that the creation and establishment of the Institute was determined by the need to develop the country's ferrous metallurgy. The Institute has united groups of metallurgical scientists, scattered in their activities, who worked in various institutes of the country. The program of the Institute, despite its youth, was wide and relevant, which can be explained by the experience, initiative and scale of the activities of the scientists who headed the departments of the institute. The origins of the creation of the scientific themes of the Institute, which covered all the major redistribution of the steel industry, are given. The major scientific developments created by the Institute’s staff for the first time in world and domestic practice, which currently form the basis of world metallurgy, are given. Outstanding scientists of the Institute created quite a few scientific schools, which even today continue to make an invaluable contribution to the development of scientific subjects, the implementation of the results of basic and applied research in metallurgical enterprises. The analysis shows that the strategic direction of development of the domestic metallurgy in the future is an evolutionary change in metallurgical technologies. In this regard, the main areas of scientific and technical support of blast furnace, steelmaking and rolling production, heat treatment of rolled products developed at the Institute are given. Examples of modern scientific developments of the Institute are given.

scholarly journals Phytochemical Diversity in Rhizomes of Three Reynoutria Species and their Antioxidant Activity Correlations Elucidated by LC-ESI-MS/MS Analysis

2019 ◽  
Author(s):  
Izabela Nawrot-Hadzik ◽  
Sylwester Ślusarczyk ◽  
Sebastian Granica ◽  
Jakub Hadzik ◽  
Adam Matkowski
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Therapeutic Effects ◽  
Similar Species ◽  
Reynoutria Japonica ◽  
Esi Ms ◽  
Total Antioxidant ◽  
First Time

One of the richest natural sources of resveratrol - the rhizome of Reynoutria japonica in East Asia is a well-known traditional herb (Hu zhang) used in various inflammatory diseases, infections, skin diseases, scald, hyperlipidemia. Although, it has been recently included in the European Pharmacopoeia, still in Europe is an untapped resource. Some of the therapeutic effects are likely to be influenced by its antioxidant properties and this in turn is frequently associated with a high stilbene content. However, some literatures suggested that other compounds than stilbenes may add to the total antioxidant capacity. Hence, the aim of this research was to examine rhizomes of R. japonica and less studied, morphologically similar species, R. sachalinensis and R. x bohemica for their phytochemical composition and antioxidant activity and to clarify the relationship between the antioxidant activity and compounds by statistical methods. HPLC/UV/ESI-MS studies of three Reynoutria species revealed 171 compounds comprising stilbenes, carbohydrates, procyanidins, flavan-3-ols, anthraquinones, phenylpropanoids, lignin oligomers, hydroxycinnamic acids, naphthalenes and their derivatives. Our studies confirmed the presence of procyanidins with high degree of polymerization, up to decamers in the rhizomes of R. japonica and brings new data on the presence of these compounds in other Reynoutria species. A procyanidin trimer digallate was described for the first time in the studied plants. Moreover, we suggested a presence of new for these species, dianthrone glycosides and previously unrecorded phenylpropanoid disaccharide esters and hydroxycinnamic acid derivatives, mainly in R. sachalinensis. Furthemore, compounds tentatively annotated as lignin oligomers were observed for the first time in studied species. The rhizomes of all Reynoutria species exhibited strong antioxidant activity. Statistical analysis demonstrated that proanthocyanidins should be considered as important contributors to the total antioxidant capacity.

scholarly journals Click beetles of the genus Melanotus Eschscholtz, 1829 (Coleoptera, Elateridae) in the collection of the Institute of Zoology of the Scientific Center of Zoology and Hydroecology of Armenia

2021 ◽  
Vol 15 (4) ◽  
pp. 145-151
Author(s):  
M. A. Marjanyan ◽  
A. A. Avetisyan
Keyword(s):  
First Record ◽  
Current Data ◽  
National Academy Of Sciences ◽  
Scientific Center ◽  
Click Beetles ◽  
The Caucasus ◽  
The Republic ◽  
Academy Of Sciences ◽  
First Time ◽  
Species Being

Aim. The study of the genus Melanotus from the collection of the Institute of Zoology of the Scientific Center of Zoology and Hydroecology of the National Academy of Sciences of the Republic of Armenia taking into account new taxonomic and chorological data.Material and Methods. Scientific material was identified after desktop processing and preparation of genitalia according to current data on the genus Melanotus.Results and Conclusion. We determined 21 species of the genus Melanotus in the collection from Europe, the Caucasus and Middle Asia, 12 species being recorded from the territory of Armenia. The collection of the genus Мelanotus in the Institute of Zoology includes species endemic to Armenia (M. gedeoni Mardjanyan, 2015, M. platiai Mardjanyan, 2015, M. khnzoriani Mardjanyan, 2015), to Tajikistan (M. fragilloides Dolin, 1988, M. vidualis Gurjeva, 1988) and to Iran (M. dichroides Platia & Gudenzi, 1999, M. richterae Mardjanyan, 2015). Three species, M. castanipes Paykull, 1800, M. fulvus Reitter, 1891 and M. sladkovi Dolin & Atamuradov, 1986 are recorded for the first time for Armenia. M. rustamovi Dolin & Atamuradov, 1987 is the first record for Tajikistan and M. persimilis Dolin & Latifi, 1988 for Turkmenistan.

scholarly journals Allium sativum Extract Chemical Composition, Antioxidant Activity and Antifungal Effect against Meyerozyma guilliermondii and Rhodotorula mucilaginosa Causing Onychomycosis

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3958 ◽  
Author(s):  
Marcel Pârvu ◽  
Cătălin A. Moţ ◽  
Alina E. Pârvu ◽  
Cristina Mircea ◽  
Leander Stoeber ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Fungal Species ◽  
Rhodotorula Mucilaginosa ◽  
Major Health Problem ◽  
Main Compound ◽  
Meyerozyma Guilliermondii ◽  
Total Oxidative Status ◽  
Potential Alternative ◽  
First Time

Onychomycosis is a major health problem due to its chronicity and resistance to therapy. Because some cases associate paronychia, any therapy must target the fungus and the inflammation. Medicinal plants represent an alternative for onychomycosis control. In the present work the antifungal and antioxidant activities of Alium sativum extract against Meyerozyma guilliermondii (Wick.) Kurtzman & M. Suzuki and Rhodotorula mucilaginosa (A. Jörg.) F.C. Harrison, isolated for the first time from a toenail onychomycosis case, were investigated. The fungal species were confirmed by DNA molecular analysis. A. sativum minimum inhibitory concentration (MIC) and ultrastructural effects were examined. At the MIC concentration (120 mg/mL) the micrographs indicated severe structural alterations with cell death. The antioxidant properties of the A. sativum extract were evaluated is a rat turpentine oil induced inflammation, and compared to an anti-inflammatory drug, diclofenac, and the main compound from the extract, allicin. A. sativum reduced serum total oxidative status, malondialdehyde and nitric oxide production, and increased total thiols. The effects were comparable to those of allicin and diclofenac. In conclusion, the garlic extract had antifungal effects against M. guilliermondii and R. mucilaginosa, and antioxidant effect in turpentine-induced inflammation. Together, the antifungal and antioxidant activities support that A. sativum is a potential alternative treatment in onychomycosis.

PROPAGATION, TROPHIC CONNECTION AND PHENOLOGY OF METCALFA PRUINOSA (SAY, 1830) (AUCHENORRHYNCHA: HEMIPTERA) IN N.N. GRYSHKO NATIONAL BOTANICAL GARDEN OF THE NATIONAL ACADEMY OF SCIENCES OF UKRAINE

2021 ◽  
Vol 14 (4) ◽  
pp. 97-105
Author(s):  
N. V. Kushnir ◽  
L. M. Bondareva
Keyword(s):  
Host Plants ◽  
Far East ◽  
Geographic Area ◽  
Botanical Garden ◽  
Complete Disappearance ◽  
Vegetable Crops ◽  
National Academy Of Sciences ◽  
The Far East ◽  
Academy Of Sciences ◽  
First Time

In May 2016, larvae of the invasive species Metcalfa pruinosa (Say, 1830) were recorded for the first time on the territory of the botanical-geographic area ‘The Far East’ in the N.N. Gryshko National Botanical Garden, National Academy of Sciences of Ukraine in Kyiv. During five years of observations, the insect managed to expand its range to other areas of the Botanical Garden and adjacent territories, where it reached a high population density. It is still the northernmost point of the insect’s detection on the territory of Ukraine. The range of M. pruinosa host plants was examined. The species lives on a large number of ornamental and synanthropic plants, fruit and vegetable crops which belong to 80 species from 55 families. The most susceptible to colonization by citrus cicada are: Rosaceae - 18 species, Aceraceae - 5 species, Oleacea e - 4 plant species. It has been found out that M. pruinosa has one generation per year. In the first decade of May, the appearance of cicada larvae is observed. The first adults are recorded in early or mid-July, depending on the meteorological conditions of the year. The complete disappearance of adults is observed at the end of August. The insect's development is limited by a minimum air temperature of 17-20 °С. In the future, it is necessary to monitor and analyze comprehensively those various factors that may affect the emergence, propagation, and survival of M. pruinosa on new territories.

scholarly journals Antioxidant potential study of some synthesized N-heterocycles

2009 ◽  
Vol 35 (2) ◽  
pp. 49-52 ◽  
Author(s):  
Mohammad Mamun Hossain ◽  
Sukanta Kumar Shaha ◽  
Foysal Aziz
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Anticancer Activity ◽  
Heterocyclic Compounds ◽  
Antioxidant Properties ◽  
Biological Properties ◽  
Antioxidant Potential ◽  
Heating Method ◽  
Dpph Method ◽  
First Time

Nitrogen containing heterocyclic compounds such as oxindoles especially isatins and their derivatives have excellent biological properties such as anti-inflammatory, antimicrobial, anticancer activity. In addition, this is first time ever we would like to report the antioxidant properties of the said class of compounds. To determine such an important bioactivity a number of halogenated oxindoles 1-3, their diaza-biphenyl derivatives (4-6), ring extended biphenyl (7) and bis-amide (8) were synthesized by classical heating method. The antioxidant activity of all the synthesized compounds was screened by DPPH method with respect to ascorbic acid. In our present investigation some of the synthesized compounds (1, 2, 3, 5 and 6) were found to be active.Keywords: Antioxidant; Isatin; OxindoleOnline 12 August 2009DOI: 10.3329/bmrcb.v35i2.2564Bangladesh Med Res Counc Bull 2009; 35: 49-52 

scholarly journals Chemical Investigation of SomeCapparisSpecies Growing in Egypt and their Antioxidant Activity

2007 ◽  
Vol 4 (s1) ◽  
pp. 25-28 ◽  
Author(s):  
Ahmed R. Hamed ◽  
Khaled A. Abdel-Shafeek ◽  
Nahla S. Abdel-Azim ◽  
Shams I. Ismail ◽  
Faiza M. Hammouda
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Evaluation ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Chemical Investigation ◽  
Dpph Radical ◽  
Densitometric Analysis ◽  
Butanol Fraction ◽  
First Time ◽  
Dpph Radical Scavenging

Capparis cartilagineaandC. desertigrowing in Egypt were investigated for their glucosiolates and rutin content. FromCapparis cartilagineafour isothiocynates were isolated and identified using GC and EI/MS techniques. These compounds were butyl isothiocyanate (1), 6-methylsulphonylhexyl isothiocyanate (2), 7-methylsulphonylheptyl isothiocyanate (3) and 5-benzylsulphonyl-4-pentenyl isothiocyanate (4). In addition to compounds (1) and (2), two other compounds were isolated and identified fromCapparis deserti. These compounds are 3-methylthiopropyl isothiocyanate (5) and [11-(2-butenylthio)6-undecenyl isothiocyanate] (6). Compounds (1), (2), (5) and (6) are reported in this study for the first time fromCapparis deserti. The main flavonoid component in the studied species was isolated and identified as rutin by comparing the data with those reported. Also, quantitative evaluation of rutin in the two species was carried out by TLC-densitometric analysis. The antioxidant activity was done using diphenylpicrylhydrazyl (DPPH) radical scavenging method. The butanol fraction fromC. cartilagineaandC. desertishowed the highest antioxidant properties.

scholarly journals The Work of Mykola Kovalskyi at the Lviv Museum of Ethnography and Art Crafts (1959–1963)

2020 ◽  
Vol 2 (1) ◽  
pp. 25
Author(s):  
Oleksandr V. Melnyk ◽  
Volodymyr V. Trofymovych ◽  
Liliia V. Trofymovych
Keyword(s):  
Young Scientist ◽  
Scientific School ◽  
National Academy Of Sciences ◽  
Organizational Activity ◽  
Source Studies ◽  
History Of ◽  
Unique Experience ◽  
The 1960S ◽  
Academy Of Sciences ◽  
First Time

The purpose of the article is to highlight the period of scientific, educational, organizational activity of the famous Ukrainian historian, the founder of the modern source studies scientific school of Ukraine — Mykola Kovalskyi at the Museum of Ethnography and Art Crafts, where he worked in the late 1950s — in the first third of the 1960s. Research methods: chronological, diachronic, classification, historical-genetic, comparative-historical. The main results: the article describes the excursion, exhibition, stock, popularization and other forms of museum work that M. Kovalskyi conducted at this time; also we can reproduce the intellectual environment at the museum through the prism of his memories; the activity of the scientist on the post of the head of the Department of Ethnography, which he occupied from the second half of 1961 to the middle of 1963, was highlighted, when he drew attention to such areas of work as reorganization of the exposition, expeditions, preparation and writing of collective monographs, concerned about the issue of scientific production, participation staff in forums, seminars, conferences, as well as staffing the department; the directions of scientific researches related to such topics as farm tools of Ukrainian peasants of the second half of the 19th and beginning of the 20th centuries were analyzed the culture and life of miners of the Lviv-Volyn coal basin; the methods of conducting a researcher of search work are revealed, which testified to the special attention to the collection of field materials and questionnaires; it is determined that during the period of work at the museum M. Kovalskyi began to develop such forms of scientific-organizational activity, which were aimed at conducting field conferences, which promoted the popularization of the best examples of Ukrainian folk art, household items, artistic crafts (for the participants were read reports about Ukrainian artistic fabrics, the use of elements of cut and folk embroidery in the clothes, thematic exhibitions were held); it is shown how contacts with foreign ethnographic institutions, in particular with the Institute of Ethnography of the Slovak Academy of Sciences, developed. Summary conclusions: scientific, excursion, stock and popularization work in the field of ethnography and artistic crafts have considerably expanded the scientific horizons of the young scientist, gave him the opportunity to join the unique experience and traditions of the school of Lviv ethnographers. Practical value: the basic provisions and factual material can be used for research on the history of Ukrainian ethnographic science, the preparation of guides and the coverage of the history of the Museum of Ethnography and Art Crafts of the Ethnology Institute National Academy of Sciences of Ukraine. Originality: the museum activity of M. Kovalskyi was covered against the backdrop of the Museum of Ethnography and Art Crafts in the late 1950s — in the first third of the 1960s. Scientific novelty: for the first time an attempt was made to study the activity of M. Kovalskyi at the Museum of Ethnography and Art Crafts in 1959 – 1963. Type of article: scientific.

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