Isolation and identification of Citrobacter freundii from chicken meat samples using cultural and molecular techniques

2018 ◽  
Vol 59 (3A) ◽  
Keyword(s):  
Chicken Meat ◽  
Molecular Techniques ◽  
Citrobacter Freundii ◽  
Isolation And Identification ◽  
Meat Samples

scholarly journals Retail chicken meats as potential sources of Clostridioides difficile in Al-Jouf, Saudi Arabia

2021 ◽  
Vol 15 (07) ◽  
pp. 972-978
Author(s):  
Ahmed Elsayed Taha Attia
Keyword(s):  
Saudi Arabia ◽  
Wide Spectrum ◽  
Human Infection ◽  
Chicken Meat ◽  
Isolation And Identification ◽  
Clostridioides Difficile ◽  
Potential Sources ◽  
Toxigenic Strains ◽  
Antibiotics Sensitivity ◽  
Meat Samples

Introduction: Presence of Clostridioides difficile in stool of food birds and animals is a risk for contamination of their meats to become potential sources of human infection. The main virulence factors of C. difficile are its resistance to antibiotics, production of toxins and spores. As far as I know, this is the first study to evaluate C. difficile prevalence in chicken meats, its toxigenic activities and antibiotics sensitivity patterns in Al-Jouf, Saudi Arabia. Methodology: Totally, 250 raw chicken meat samples were examined. Standard microbiological and biochemical procedures were used for C. difficile isolation and identification. The suspected colonies were tested by L-proline and C. difficile test kits then confirmed by Vitek 2 compact system. Xpect C. difficile toxin A/B test was used to detect A/B toxins production. Antibiotics susceptibility patterns were detected by Epsilon tests. Results: C. difficile was isolated from 11/250 (4.40%) chicken meat samples; 5/65 (7.69%) legs, 3/65 (4.61%) thighs, 2/60 (3.33%) wings and 1/60 (1.67%) breasts (p = 0.4). All isolates were non-toxigenic. Although all isolates were vancomycin sensitive, some isolates were intermediate/resistant to metronidazole, tetracycline, clindamycin or moxifloxacin antibiotics with variable degrees. Conclusions: C. difficile might contaminate retail chicken meats. Although low level of contamination by non-toxigenic strains was detected, chicken meats should be investigated as C. difficile infection sources for humans especially elders, immune-compromised and long terms wide spectrum antibiotics-used persons. Decreased sensitivity of C. difficile to antibiotics is emerging.

scholarly journals Three-Year Longitudinal Study: Prevalence of Salmonella Enterica in Chicken Meat is Higher in Supermarkets than Wet Markets from Mexico

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 264
Author(s):  
Iván D. Regalado-Pineda ◽  
Rene Rodarte-Medina ◽  
Carolina N. Resendiz-Nava ◽  
Cinthia E. Saenz-Garcia ◽  
Pilar Castañeda-Serrano ◽  
...  
Keyword(s):  
Recovery Rate ◽  
Salmonella Enterica ◽  
Foodborne Pathogen ◽  
International Health ◽  
Chicken Meat ◽  
Poultry Meat ◽  
Isolation And Identification ◽  
Salmonella Infections ◽  
Surveillance Programs ◽  
Meat Samples

Worldwide, chicken meat is considered one of the main sources of Salmonella enterica in humans. To protect consumers from this foodborne pathogen, international health authorities recommend the establishment of continuous Salmonella surveillance programs in meat. However, these programs are scarce in many world regions; thus, the goal of the present study was to perform a longitudinal surveillance of S. enterica in chicken meat in Mexico. A total of 1160 samples were collected and analyzed monthly from 2016 to 2018 in ten chicken meat retailers (supermarkets and wet markets) located in central Mexico. The isolation and identification of S. enterica was carried out using conventional and molecular methods. Overall, S. enterica was recovered from 18.1% (210/1160) of the chicken meat samples. Remarkably, during the three years of evaluation, S. enterica was more prevalent (p < 0.0001) in supermarkets (27.2%, 158/580) than in wet markets (9.0%, 52/580). The study was 3.8 times more likely (odds ratio = 3.8, p < 0.0001) to recover S. enterica from supermarkets than wet markets. Additionally, a higher prevalence (p < 0.05) of this pathogen was observed during the spring, summer, autumn, and winter in supermarkets compared with wet markets. Moreover, the recovery rate of S. enterica from supermarkets showed a gradual increase from 20.78% to 42% (p < 0.0001) from 2016 to 2018. Interestingly, no correlation (p > 0.05) was observed between the S. enterica recovery rate in chicken meat and reported cases of Salmonella infections in humans. Higher levels of S. enterica in chicken meat retailed in supermarkets are not unusual; this phenomenon has also been reported in some European and Asian countries. Together, these results uncover an important health threat that needs to be urgently addressed by poultry meat producers and retailers.

scholarly journals Bacteriological Assessment of Chicken Meat, Chicken Meat Products and Its Impact of Human Enteric Infections in Taif Governorate

2019 ◽  
pp. 1-10
Author(s):  
Ahmed M. A. Mansour
Keyword(s):  
Campylobacter Jejuni ◽  
Meat Products ◽  
Enterobacter Aerogenes ◽  
Chicken Meat ◽  
Permissible Limit ◽  
Klebsiella Aerogenes ◽  
Citrobacter Freundii ◽  
Morganella Morganii ◽  
Providencia Stuartii ◽  
Meat Samples

From different shops and supermarkets at Taif governorate in KSA; a total number of 105 samples were collected. They were 35 samples from raw chicken meat, 35 samples from frozen chicken meat burger and 35 samples from chicken meat luncheon. The samples were examined for their organoleptic and bacteriological quality; the results revealed that, 8.6% and 2.9% of the examined raw chicken meat and frozen chicken burger were unaccepted while all examined samples of chicken-luncheon were accepted. The bacteriological  examination revealed that, the bacterial counts in frozen chicken burger samples were higher than that detected in raw chicken meat and chicken luncheon samples whereas 51.4%, of the frozen chicken burger were exceeded the permissible limit, but 45.7% of the raw chicken meat samples exceeded the permissible limit, while 20% from the chicken luncheon samples exceeded the permissible limit, moreover, E. coli, Enterobacter aerogenes, Enterobacter cloaca, Klebsiella aerogenes, Citrobacter freundii, Proteus vulgaris, Morganella morganii, Providencia stuartii and Providencia rettergii could be isolated from the examined samples of raw chicken meat and frozen chicken burger in varying percentages ranged from 2.86 to 22.85% and 2.86 to 20.00% respectively, while Enterobacter aerogenes, Klebsiella aerogenes, Citrobacter freundii, P. vulgaris and Morganella morganii only were detected in chicken luncheon in a percentage varying from 5.17 to 17.14%. Furthermore, Campylobacter jejuni were isolated in a percentage of 14.3, 8.8 and 2.9% from the former examined samples respectively, while Salmonella organism were detected in raw chicken meat samples in a percentage of 5.7% but failed to be isolated from frozen chicken burger and chicken luncheon samples. The relationship between total aerobic count and the incidence of Campylobacter jejuni and Salmonella pathogens as well as the public health significance of the isolated organisms and preventive measures to improve the quality of the products were discussed.

scholarly journals Quorum sensing systems and related virulence factors in Pseudomonas aeruginosa isolated from chicken meat and ground beef

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Gökhan İnat ◽  
Belgin Sırıken ◽  
Ceren Başkan ◽  
İrfan Erol ◽  
Tuba Yıldırım ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Protease Activity ◽  
Ground Beef ◽  
Homoserine Lactone ◽  
Chicken Meat ◽  
Molecular Techniques ◽  
Isolation And Identification ◽  
Conventional Culture

AbstractThe objective of this study was to evaluate 50 [chicken meat (n = 45) and ground beef (n = 5)] Pseudomonas aeruginosa isolates to determine the expression of the lasI and rhl QS systems, related virulence factors, and the presence of N-3-oxo-dodecanoyl homoserine lactone (AHL: 3-O-C12-HSL). For the isolation and identification of P. aeruginosa, conventional culture and oprL gene-based molecular techniques were used. In relation to QS systems, eight genes consisting of four intact and four internal (lasI/R, rhlI/R) genes were analyzed with PCR assay. The two QS systems genes in P. aeruginosa isolates from ground beef (80.00%) and chicken meat (76.00%) were present at quite high levels. The 3-O-C12-HSL was detected in 14.00% of the isolates. Both biofilm formation and motility were detected in 98.00% of the isolates. Protease activity was determined in 54.00% of the isolates. Pyocyanin production was detected in 48.00% of the isolates. The las system scores strongly and positively correlated with the rhl system (p ˂ .01). PYA moderately and positively correlated with protease (p ˂ .05). In addition, there was statistically significance between lasI and protease activity (p < .10), and rhlI and twitching motility (p < .10). In conclusion, the high number of isolates having QS systems and related virulence factors are critical for public health. Pyocyanin, protease, and biofilm formation can cause spoilage and play essential role in food spoilage and food safety.

scholarly journals Phylogenetic Analysis and Antibiotics Resistance of Listeria Monocytogenes Contaminating Chicken Meat in Surabaya, Indonesia

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Eduardus Bimo Aksono ◽  
Katty Hendriana Priscilla Riwu ◽  
A. T. Soelih Estoepangestie ◽  
Herinda Pertiwi
Keyword(s):  
Phylogenetic Analysis ◽  
Antibiotic Resistance ◽  
Listeria Monocytogenes ◽  
Selective Medium ◽  
Chicken Meat ◽  
Antibiotics Resistance ◽  
Isolation And Identification ◽  
Traditional Markets ◽  
Meat Samples ◽  
New Generation

The objective of this study was to identify the phylogenetic analysis and antibiotic resistance of Listeria monocytogenes contaminating chicken meat in Surabaya. 60 chicken meat samples were collected from supermarkets, mobile vendors, and traditional markets in Surabaya. A selective medium is used for isolation and identification of Listeria monocytogenes by chopping 25 grams of the chicken meat and to put it into the sterilized Erlenmeyer flasks. Some methods were used for the identification procedures, such as biochemical and morphological tests, antibiotic resistance test, PCR, and sequencing; also a phylogenetic analysis was conducted by a neighbor-joining analysis using Genetix Mac ver 8.0 with hlyA genes of Listeria monocytogenes recorded in GenBank, such as Lineage I (KC808543), Lineage II (AY229462, AY229346, AY229499, and AY229404), Lineage III (KJ504139, HQ686043, KJ504116, and DQ988349), and Lineage IV (EU840690, EF030606). The result shows that the prevalence of L. monocytogenes in Surabaya contaminating the chicken meat samples from the supermarkets was 10% (2/20), from the mobile vendors was 0/20 (0%), and from the traditional markets was 5% (1/20). It was seen from the band at 456 bp fragment. Furthermore, three isolates found in Surabaya were included in the new lineages which were resistant to old-generation antibiotics such as sulfamethonazole-trimetophrim (SXT) and amoxyllin sulbactam (MAS), but they were still sensitive to new-generation antibiotics such as cefotaxime (CTX) and meropenem (MEM).

scholarly journals Bacteriological Study of Frozen Meat in Taif Governorate m Saudi Arabia

2004 ◽  
Vol 9 (2) ◽  
pp. 51
Author(s):  
A. Altalhi ◽  
M. Albashan
Keyword(s):  
Chicken Meat ◽  
Bacteriological Examination ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Bacteriological Study ◽  
Random Samples ◽  
Plate Counts ◽  
Camel Meat ◽  
Meat Samples

This study was carried out on I25 random samples of frozen meat collected aseptically from different shops and supermarkets distributed in Taif governorate. All samples were subjected to bacteriological examination for aerobic plate counts of Enterobactcriaceae, Staphylococcus aureus, and Lactobacillaceae, and for isolation and identification the strains isolated bacteriologically. The aerobic plate counts ranged from 3 x 10 4 to 107 cfu.g-1. The couan for Entcrobacteriaceae, Staphylococcus- aurcus, and Lactobacillaceae were (5 x IO 2to 2 x 1O 6cfu.g-1), (6 xl0 3 to 10 6 cfu.g-1), (6 x1O 3 to 3x lO 6cfu.g-1 ), respectively. The bacterial counts of frozen chicken meat ranged from (3xl0 3) to 20 x10 5/ cm-2. The number of strains isolated from frozen meat samples were 167, 67, 79, 76, and 87 for mutton, camel meats, imported beef, local chicken meats and imported chicken meat respectively. The percentages of bacterial isolates from frozen mutton were higher than those from frozen camel meat and beef. The frequency of isolation of different bacterial strains from imported frozen chicken meat was higher than that from local frozen chicken meat.

scholarly journals Prediction of Thiol Group Changes in Minced Raw and Cooked Chicken Meat with Plant Extracts—Kinetic and Neural Network Approaches

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1647
Author(s):  
Anna Kaczmarek ◽  
Małgorzata Muzolf-Panek
Keyword(s):  
Plant Extracts ◽  
Predictive Models ◽  
External Validation ◽  
Thiol Group ◽  
Chicken Meat ◽  
Validation Dataset ◽  
Heat Treated ◽  
Different Temperatures ◽  
Meat Samples ◽  
Network Approaches

The aim of the study was to develop predictive models of thiol group (SH) level changes in minced raw and heat-treated chicken meat enriched with selected plant extracts (allspice, basil, bay leaf, black seed, cardamom, caraway, cloves, garlic, nutmeg, onion, oregano, rosemary, and thyme) during storage at different temperatures. Meat samples with extract addition were stored under various temperatures (4, 8, 12, 16, and 20 °C). SH changes were measured spectrophotometrically using Ellman’s reagent. Samples stored at 12 °C were used as the external validation dataset. SH content decreased with storage time and temperature. The dependence of SH changes on temperature was adequately modeled by the Arrhenius equation with average high R2 coefficients for raw meat (R2 = 0.951) and heat-treated meat (R2 = 0.968). Kinetic models and artificial neural networks (ANNs) were used to build the predictive models of thiol group decay during meat storage. The obtained results demonstrate that both kinetic Arrhenius (R2 = 0.853 and 0.872 for raw and cooked meat, respectively) and ANN (R2 = 0.803) models can predict thiol group changes in raw and cooked ground chicken meat during storage.

Ultrasensitive determination of chloramphenicol in pork and chicken meat samples using a portable electrochemical sensor: effects of 2D nanomaterials on the sensing performance and stability

2021 ◽  
Author(s):  
Ngo Xuan Dinh ◽  
Tuyet Nhung Pham ◽  
Tran Quang Huy ◽  
Do Quang Trung ◽  
Pham Anh Tuan ◽  
...  
Keyword(s):  
Electrochemical Sensor ◽  
Food Samples ◽  
Chicken Meat ◽  
Electrochemical Sensing ◽  
Portable Sensors ◽  
2D Nanomaterials ◽  
Meat Samples ◽  
Sensing Performance

This work contributes to a deeper understanding of the effects of functional 2D nanomaterials on the electrochemical sensing performance of SPE-based portable sensors for the rapid, accurate, and on-site determination of CAP in food samples.

scholarly journals Molecular and HPLC-based approaches for detection of aflatoxin B1 and ochratoxin A released from toxigenic Aspergillus species in processed meat

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Abdelazeem M. Algammal ◽  
Mahmoud E. Elsayed ◽  
Hany R. Hashem ◽  
Hazem Ramadan ◽  
Norhan S. Sheraba ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Meat Products ◽  
Its Region ◽  
Processed Meat ◽  
Isolation And Identification ◽  
Beef Meat ◽  
Meat Samples ◽  
Aflatoxin B ◽  
Minced Meat ◽  
Mold Count

Abstract Background Meat-products are considered an enriched media for mycotoxins. This study aimed to investigate the prevalence of toxigenic Aspergillus species in processed meat samples, HPLC-quantitative measurement of aflatoxin B1 and ochratoxin A residues, and molecular sequencing of aflR1 and pks genes. One hundred and twenty processed beef meat specimens (basterma, sausage, and minced meat; n = 40 for each) were collected from Ismailia Province, Egypt. Samples were prepared for total mold count, isolation, and identification of Aspergillus species. All samples were analyzed for the production of both Aflatoxin B1 and Ochratoxin A mycotoxins by HPLC. Molecular identification of Aspergillus flavus and Aspergillus ochraceus was performed using PCR amplification of the internal transcribed spacer (ITS) region; furthermore, the aflR1 and pks genes were sequenced. Results The total mold count obtained from sausage samples was the highest one, followed by minced meat samples. The prevalence of A. flavus was (15%), (7.5%), and (10%), while the prevalence of A. ochraceus was (2.5%), (10%), and (0%) in the examined basterma, sausage, and minced meat samples, respectively. Using PCR, the ITS region was successfully amplified in all the tested A. flavus and A. ochraceus strains. Aflatoxin B1 was detected in six basterma samples (15%). Moreover, the ochratoxin A was detected only in four sausage samples (10%). The aflR1 and pks genes were amplified and sequenced successfully and deposited in the GenBank with accession numbers MF694264 and MF694264, respectively. Conclusions To the best of our knowledge, this is the first report concerning the HPLC-Molecular-based approaches for the detection of aflatoxin B1 and ochratoxin A in processed beef meat in Egypt. The production of aflatoxin B1 and ochratoxin A in processed meat constitutes a public health threat. Aflatoxin B1 is commonly associated with basterma samples. Moreover, ochratoxin A was detected frequently in sausage samples. The routine inspection of mycotoxins in processed meat products is essential to protect human consumers.

scholarly journals Helicobacter pullorum Isolated from Fresh Chicken Meat: Antibiotic Resistance and Genomic Traits of an Emerging Foodborne Pathogen

2015 ◽  
Vol 81 (23) ◽  
pp. 8155-8163 ◽  
Author(s):  
Vítor Borges ◽  
Andrea Santos ◽  
Cristina Belo Correia ◽  
Margarida Saraiva ◽  
Armelle Ménard ◽  
...  
Keyword(s):  
Membrane Filter ◽  
Acquired Resistance ◽  
Foodborne Pathogen ◽  
Meat Products ◽  
Chicken Meat ◽  
Filter Method ◽  
Type Vi Secretion System ◽  
Genetic Traits ◽  
Content Type ◽  
Meat Samples

ABSTRACTMeat and meat products are important sources of human intestinal infections. We report the isolation ofHelicobacter pullorumstrains from chicken meat. Bacteria were isolated from 4 of the 17 analyzed fresh chicken meat samples, using a membrane filter method. MIC determination revealed that the four strains showed acquired resistance to ciprofloxacin; one was also resistant to erythromycin, and another one was resistant to tetracycline. Whole-genome sequencing of the four strains and comparative genomics revealed important genetic traits within theH. pullorumspecies, such as 18 highly polymorphic genes (including a putative new cytotoxin gene), plasmids, prophages, and a complete type VI secretion system (T6SS). The T6SS was found in three out of the four isolates, suggesting that it may play a role inH. pullorumpathogenicity and diversity. This study suggests that the emerging pathogenH. pullorumcan be transmitted to humans by chicken meat consumption/contact and constitutes an important contribution toward a better knowledge of the genetic diversity within theH. pullorumspecies. In addition, some genetic traits found in the four strains provide relevant clues to how this species may promote adaptation and virulence.

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