scholarly journals Detection and Identification of Novel Genes from Fungi Isolated from Wastewater

2021 ◽  
pp. 2509-2513
Author(s):  
Mohammad Ibrahim Khalil
Keyword(s):  
Neurospora Crassa ◽  
Gene Bank ◽  
Trichoderma Asperellum ◽  
Contamination Sources ◽  
Detection And Identification ◽  
5.8S Rrna ◽  
Long Time ◽  
Fusarium Falciforme ◽  
Fungal Genes ◽  
First Time

     The environment in Mosul city is very rich, containing a wide variety of microorganisms which have not been recognised for a long time. Five new fungal genes were identified and registered for the first time in the gene bank. These included Fusarium falciforme 2020-06-MIK-F1 genes for 5.8S rRNA with Accession no. LC555741, Nectriaceae sp. 2020-06-MIK-F2 genes for ITS1 with Accession no. LC555742, Trichoderma asperellum MIK3 genes for 5.8S rRNA with Accession no. LC575020, Penecillum sp. MIK4 genes for 5.8S rRNA with Accession no. LC575021, and Neurospora crassa MIK5 genes for 5.8S rRNA with Accession no. LC575022.   These fungal genes were isolated from wastewater of Khosr river in Mosul city/ Iraq, which has many contamination sources.

Direct imaging of charge transfer

1996 ◽  
Vol 54 ◽  
pp. 680-681
Author(s):  
Yimei Zhu ◽  
J. Tafto
Keyword(s):  
Charge Transfer ◽  
Electron Diffraction ◽  
Scattering Amplitude ◽  
High Temperature Superconductors ◽  
Charge Carriers ◽  
Image Charge ◽  
Net Charge ◽  
Long Time ◽  
Electron Holes ◽  
First Time

The electron holes confined to the CuO2-plane are the charge carriers in high-temperature superconductors, and thus, the distribution of charge plays a key role in determining their superconducting properties. While it has been known for a long time that in principle, electron diffraction at low angles is very sensitive to charge transfer, we, for the first time, show that under a proper TEM imaging condition, it is possible to directly image charge in crystals with a large unit cell. We apply this new way of studying charge distribution to the technologically important Bi2Sr2Ca1Cu2O8+δ superconductors.Charged particles interact with the electrostatic potential, and thus, for small scattering angles, the incident particle sees a nuclei that is screened by the electron cloud. Hence, the scattering amplitude mainly is determined by the net charge of the ion. Comparing with the high Z neutral Bi atom, we note that the scattering amplitude of the hole or an electron is larger at small scattering angles. This is in stark contrast to the displacements which contribute negligibly to the electron diffraction pattern at small angles because of the short g-vectors.

Assessment of recreational use of specially protected natural territories of Tatishchevsky district of Saratov region

2019 ◽  
Vol 27 (2) ◽  
pp. 117-127
Author(s):  
Yulia M Andriyanova ◽  
Irina V Sergeeva ◽  
Yulia M Mokhonko ◽  
Natalia N Gusakova
Keyword(s):  
Natural Resources ◽  
Protected Areas ◽  
Saratov Region ◽  
Future State ◽  
Forest Phytocenoses ◽  
Long Time ◽  
Main Components ◽  
First Time ◽  
Recreational Use ◽  
Specially Protected Natural Territories

The influence of recreation being a set of measures to restore health and recreation, on the main components of forest phytocenoses in specially protected natural territories of the Tatishchevsky district of the Saratov region has been studied for the first time. These phytocenoses have been intensively used for tourism for a long time. The intensity and visits activity of protected areas has been determined; the recreational capacity of territorial objects has been studied. The degree of forest landscapes has been revealed in specially protected natural territories. The findings allow predicting the future state of the natural resources of the Saratov region and can be taken into account when assessing their optimal use.

scholarly journals Clinical significance of late diastolic tissue doppler parameters after onset of STEMI: from the view point of the timing of the echocardipography

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
N Iwahashi ◽  
J Kirigaya ◽  
M Horii ◽  
T Abe ◽  
E Akiyama ◽  
...  
Keyword(s):  
Tissue Doppler ◽  
Atrial Function ◽  
Diastolic Velocity ◽  
Long Time ◽  
Group A ◽  
St Elevation ◽  
Group B ◽  
First Time

Abstract Background The early transmitral flow velocity (E) divided by the early diastolic velocity of the mitral valve annulus (e') is referred to as the “E/e' ratio,” is useful even for ST elevation acute myocardial infarction (STEMI). However, the role of late diastolic velocity (a') which reveals the atrial function for STEMI is still unclear. Objectives We evaluated the clinical usefulness of tissue Doppler including atrial function for a first-time STEMI by long time follow up. Furthermore, we evaluated the meaning of each parameters by performing immediately after PCI or 2 weeks later. Methods We treated consecutive 571 first-time STEMI patients by immediate PCI within 12 hours after onset, and we examined 270 patients at immediately after PCI (GroupA, 65 years, 250 male) and 301 patients at 2 weeks after onset (GroupB, 64 years, 243 male). We examined trans mitral flow and TDI, then defined E/e' as LV filling pressure and A/a' as left atrial function. We followed them for a long time (>5 years). The primary end point (PE) was cardiac death or re-admission for heart failure (HF). Results We followed the patients in Group A for 10 years, Group B for 5 years. PE occurred in 64 patients in GroupA during 10 years, and 45 patients in GroupB during 5 years. We analyzed the univariate and multivariate Cox hazard analyses and we compared e' and a', E/e' and A/a' (Table). In GroupA, a' and A/a' were the independent predictors, on the other hand neither a' nor A/a' were the predictors in GroupB. E/e' was an independent predictor both in GroupA and B. Conclusion TDI parameters have different meanings by the timing of echocardiography after onset of a first-time STEMI. These results demonstrated that atrial dysfunction immediately after onset of STEMI suggests the poor prognosis after STEMI. Cox Hazard Proportional Analysis Funding Acknowledgement Type of funding source: None

General method for cloning Neurospora crassa nuclear genes by complementation of mutants

1985 ◽  
Vol 5 (9) ◽  
pp. 2272-2278
Author(s):  
R A Akins ◽  
A M Lambowitz
Keyword(s):  
Neurospora Crassa ◽  
Genomic Library ◽  
Selection Procedure ◽  
Nuclear Genes ◽  
Shuttle Vectors ◽  
Sib Selection ◽  
First Time ◽  
Very High ◽  
Restriction Site Polymorphisms ◽  
General Method

We have developed a sib selection procedure for cloning Neurospora crassa nuclear genes by complementation of mutants. This procedure takes advantage of a modified N. crassa transformation procedure that gives as many as 10,000 to 50,000 stable transformants per microgram of DNA with recombinant plasmids containing the N. crassa qa-2+ gene. Here, we describe the use of the sib selection procedure to clone genes corresponding to auxotrophic mutants, nic-1 and inl. The identities of the putative clones were confirmed by mapping their chromosomal locations in standard genetic crosses and using restriction site polymorphisms as genetic markers. Because we can obtain very high N. crassa transformation frequencies, cloning can be accomplished with as few as five subdivisions of an N. crassa genomic library. The sib selection procedure should, for the first time, permit the cloning of any gene corresponding to an N. crassa mutant for which an appropriate selection can be devised. Analogous procedures may be applicable to other filamentous fungi before the development of operational shuttle vectors.

scholarly journals Patients' anxiety before cardiac catheterization

2010 ◽  
Vol 8 (4) ◽  
pp. 483-487 ◽  
Author(s):  
Leandro Loureiro Buzatto ◽  
Suely Sueko Viski Zanei
Keyword(s):  
Cardiac Catheterization ◽  
Patient Information ◽  
Scientific Publications ◽  
Patient Understanding ◽  
Post Procedure ◽  
Long Time ◽  
On Line ◽  
First Time ◽  
Multiprofessional Approach ◽  
Pharmacologic Therapies

ABSTRACT Objective: To identify researches related to anxiety and strategies to reduce it in patients who are in the pre-cardiac catheterization period. Methods: A bibliographic research was carried out in the on line databases of PubMed, MedLine, CINAHL, LILACS and SciELO, from 1997 to 2009 in MedLine and from 1999 to 2009 in the others databases. The boolean expressions “and” and “or” has been used with the descriptors in Portuguese and in English. The inclusion of discerning was related about the presence, level and workable of the anxiety from the period of pre-cardiac catheterization. Results: Coping most of 17 researches selected were in American scientific publications, with experimental-descriptive studies. The possibilities of intercurrence and/or complications during and post-procedure, diagnostic, possibility of bad prognostic, being alone during the waiting, the first time submission the procedure, lost information and/or orientation and long time waiting could cause anxiety in pre-cardiac catheterization. Maintenance of escort and family alongside the patient, information adapted to the patient understanding level, overcoming traumas and difficulties with a multiprofessional approach, pharmacologic and non pharmacologic therapies were strategies to reduce the anxiety. Conclusions: The nurses are responsible to provide a humanized assistance to offer a fast recuperation, minimize traumas of the hospitalization and the procedure. The knowledge of the causes and the strategies are fundamental to reduce the level of anxiety in pre-catheterization cardiac.

scholarly journals First Results from Insemination With Sex-Sorted Semen in Dairy Heifers in Macedonia

2015 ◽  
Vol 38 (1) ◽  
pp. 107-111
Author(s):  
Ljupche Kochoski ◽  
Zoran Filipov ◽  
Ilcho Joshevski ◽  
Stevche Ilievski ◽  
Filip Davkov
Keyword(s):  
Pregnancy Rate ◽  
Transrectal Ultrasound ◽  
Gestation Length ◽  
Dairy Heifers ◽  
Holstein Friesian ◽  
First Results ◽  
Long Time ◽  
The Usa ◽  
Normal Gestation ◽  
First Time

Abstract Science has been searching for a long time for a reliable method for controlling the sex of mammalian offspring. Recently, the application of specific modern cellular methodologies has led to the development of a flow cytometric system capable of differentiating and separating living X- and Y-chromosome-bearing sperm cells in amounts suitable for AI and therefore, commercialization of this sexing technology. The aim of this work was to present the first results of heifers that introduce bovine AI with sex sorted semen, for the first time in Macedonia. Insemination with sex sorted cryopreserved semen (2×106 spermatozoa per dose) imported from the USA was done at two dairy farms in ZK Pelagonija. In total, 74 heifers (Holstein Friesian) were inseminated. Inseminations were carried out in a timely manner following a modified OvSynch protocol. During the insemination, the sperm was deposited into the uterine horn ipsi lateral to the ovary where a follicle larger than 1.6 cm was detected by means of transrectal ultrasound examination. Pregnancy was checked by ultrasound on day 30 after the insemination. Overall, the average pregnancy rate in both farms was 43,24% (40,54% and 45,95%, for farm 1 and farm 2, respectively). All pregnant heifers delivered their calves following a normal gestation length (274,3 days in average) and of the 32 born calves, 30 (93,75%) were female. In conclusion, since the first results from inseminations with sex-sorted semen in dairy heifers in Macedonia are very promising, the introduction of this technique may bring much benefit to the local dairy sector. Average pregnancy rate seems similar with results obtained following ‘regular’ inseminations, notwithstanding the relatively low number of spermatozoa per insemination dose. Due to the latter, we however recommend inseminations only to be carried out by experienced technicians followinga TAI protocol and ultrasound examinations of the ovaries prior to insemination.

scholarly journals The War of Khalkhin Gol in 1939 and the German-Soviet Nonaggression Pact

2018 ◽  
Vol 28 (4) ◽  
pp. 585-594 ◽  
Author(s):  
Alexander Sukhodolov ◽  
Tuvd Dorj ◽  
Yuriy Kuzmin ◽  
Mikhail Rachkov
Keyword(s):  
Foreign Policy ◽  
Second World War ◽  
Historical Science ◽  
World War ◽  
The World ◽  
Long Time ◽  
History Of ◽  
First Time ◽  
Further Development ◽  
Second World

For the first time in Russian historiography, the article draws attention to the connection of the War of Khalkhin Gol in 1939 and the conclusion of the German-Soviet Nonaggression Pact of 1939. For a long time, historical science considered these two major events in the history of the USSR and history of the world individually, without their historic relationship. The authors made an attempt to provide evidence of this relationship, showing the role that surrounding and defeating the Japanese army at Khalkhin Gol in August 1939 and signing in Moscow of the German-Soviet Nonaggression Pact played in the history of the world. The study analyzes the foreign policy of the USSR in Europe, the reasons for the failure in the conclusion of the Anglo-Franco-Soviet military union in 1939 and the circumstances of the Pact. It shows the interrelation between the defeat of the Japanese troops at Khalkhin Gol and the need for the Soviet-German treaty. The authors describe the historic consequences of the conclusion of the pact for the further development of the Japanese-German relations and the course of the Second World War. They also present the characteristics of the views of these historical events in the Russian historiography.

scholarly journals Estimation of a functional condition of a fetus at pregnancy and in delivery

2003 ◽  
Vol 52 (2) ◽  
pp. 110-115
Author(s):  
I. Y. Kogan ◽  
А. A. Poljanin ◽  
N. G. Pavlova
Keyword(s):  
Functional State ◽  
Fetal Heart ◽  
Functional Condition ◽  
The State ◽  
Heart Sounds ◽  
Pregnancy And Childbirth ◽  
Long Time ◽  
First Time

The lecture provides information on approaches to the assessment of fetal cardiotocography during pregnancy and childbirth. Assessment of the functional state of the fetus during pregnancy and childbirth remains one of the most difficult tasks of perinatology and obstetrics. The question of the state of the intrauterine fetus has been of interest to obstetricians for a long time. For the first time, the possibility of auscultation of fetal heart sounds was reported in 1818.

“Information Isn’t Knowledge”: Denis Rougemont on the Verge of the Information Society

2021 ◽  
Vol 35.5 ◽  
pp. 83-89
Author(s):  
Daniil T. Baboshin
Keyword(s):  
Everyday Life ◽  
Information Society ◽  
Information Technologies ◽  
Cognitive Activity ◽  
Social Experiment ◽  
Spiritual Values ◽  
Long Time ◽  
Definition Of ◽  
Definition Of Information ◽  
First Time

Today it’s not possible to deny the approach of the new epoch – the epoch of the information society. The high technologies have infiltrated the total scope of the everyday life of modern people. In 2020 our civilization confronted the new, but for a long time anticipated, challenge, - mass introduction of distant education in schools and universities. We still will have to comprehend the results of this social experiment in the nearest future. Still one fact arises no doubts: information nowadays is the product that is widely and easily (perhaps, too easily) accessible, but real knowledge remains the lot of the few, and even tend to marginalize. Forty years ago the stated problems became the issue of the studies of the Swiss philosopher Denis de Rougemont. His conclusions turn out to be more and more relevant with the acceleration of the process of culture, communications and education digitalization. His article “Information Isn’t Knowledge” has been published in Russian for the first time. The article deals with the issues of information technologies integration into the human cognitive activity, its influence on the thinking process and cultural, ethic and spiritual values formation. Denis de Rougemont step by step reveals the definition of information technologies, their application in various areas of human existence, their ability to compete with personality and the consequences of their integration in everyday life. These speculations become especially valuable in the era of the triumph for information society and global computerization.

IS ERROR FRAUD?

PEDIATRICS ◽  
1989 ◽  
Vol 84 (4) ◽  
pp. A64-A64
Author(s):  
J. F. L.
Keyword(s):  
Massachusetts Institute Of Technology ◽  
Bad Faith ◽  
Real Science ◽  
Long Time ◽  
The Government ◽  
Institute Of Technology ◽  
Witch Hunts ◽  
First Time ◽  
Published Research ◽  
The Way

Prof. David Baltimore of the Massachusetts Institute of Technology is under attack by Representative John Dingell of Michigan. Why should anyone outside of the Government or basic biomedical research care? Dr. Baltimore's reputation is at stake, but the rest of us will be affected by the outcome of these investigations as well. What has come under a legislative cloud for the first time in a very long time, perhaps ever in this country, is the legitimacy of the scientific method itself. This is an immediate and serious threat to science and medicine. The N.I.H. will have the last word on Dr. Baltimore's published research. But as I understand the Congressman's case, it is that published science must be free of error, and that error itself indicates bad faith and fraudulent intent. This is wrong. Published error is at the heart of any real science. We scientists love to do experiments that show our colleagues to be wrong and, if they are any good, they love to show us to be wrong in turn. By this adversarial process, science reveals the way nature actually works. If we as a country make science a field for only those who enjoy a good lawsuit, we will have shut the door on our future as a technologically serious nation. Clearly Congress cannot wish to do this. I would welcome a Congressional initiative to deal with fraud as such, but I fear that the way Dr. Baltimore is being treated means that witch-hunts are in the offing.

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