Essential Oils of Aerial Parts of Anaphalis margaritacea (L.) Benth.,Salvia leucantha Cav. and Thymus linearis Benth. From Uttarakhand Himalaya: Chemical Constituents and Antibacterial Activity

The components present in the essential oils extracted from Anaphalis margaritacea (L.) Benth., Salvia leucantha Cav. and Thymus linearis Benth. were identified by GC and GC/MS analysis. The main compounds present in the oils were aromadendrene (20.0%) in S. leucantha, Phellandrene (12.5%), α-thujene (12.0%) in A. margaritacea and thymol (50.0%) in T. linearis. The antibacterial activity of all the three oils against E. coli, P. aeruginosa, S. aureus and S. typhi, has been studied. In the antibacterial activity S. typhi is the most resistant bacteria to all the tested oils whereas essential oil of T. linearis showed highest antibactericidal activity against S. aureus in diffusion method.

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Essential Oil Yield Pattern and Antibacterial and Insecticidal Activities ofTrachyspermum ammiandMyristica fragrans

Scientifica ◽

10.1155/2016/1428194 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Rajgovind Soni ◽

Gaurav Sharma ◽

Nakuleshwar Dut Jasuja

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Diffusion Method ◽

Bacterial Strains ◽

E Coli ◽

Chromatography Analysis ◽

Alpha Pinene ◽

Gas Chromatography Analysis ◽

Insecticidal Activities

Two Indian spices,Trachyspermum ammiandMyristica fragrans,were studied for their essential oil (EO) yielding pattern, insecticidal activity, antibacterial activity, and composition. The essential oils (EOs) ofT. ammi(1.94±30 mL/100 gm) andM. fragrans(5.93±90 mL/100 gm) were extracted using hydrodistillation method. In Gas Chromatography analysis, the beta-pinene, alpha-pinene, alpha-p-menth-1-en-4-ol, Limonene, and elemicin were found as major constituents ofT. ammiessential oil whereasM. fragransessential oil mostly contains Gamma-Terpinolene, p-Cymene, Thymol, and beta-pinene. The insecticidal activities of EO were demonstrated using LC50values againstPlodia interpunctellaand EO ofT. ammiwas found comparatively more effective than EO ofM. fragrans.Further, individual EO and combination of essential oil were examined for antibacterial activity against three Gram (−) bacterial strains (E. coli-MTCC 443,P. vulgaris-MTCC 1771, andK. pneumoniae-MTCC number 7028) and three Gram (+) bacterial strains (S. aureus-MTCC 3381,B. subtilis-MTCC 10619, andB. megaterium-MTCC 2412) by well agar diffusion method. The essential oil in combination (CEO) exhibited higher antibacterial activity as compared with individual essential oils.

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Chemical Composition and Antibacterial Activity of Essential Oils from the Algerian Endemic Origanum glandulosum Desf. against Multidrug-Resistant Uropathogenic E. coli Isolates

Antibiotics ◽

10.3390/antibiotics9010029 ◽

2020 ◽

Vol 9 (1) ◽

pp. 29 ◽

Cited By ~ 5

Author(s):

Larbi Zakaria Nabti ◽

Farida Sahli ◽

Hocine Laouar ◽

Ahmed Olowo-okere ◽

Joice Guileine Nkuimi Wandjou ◽

...

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Multidrug Resistant ◽

Diffusion Method ◽

Resistant Bacteria ◽

E Coli ◽

Broth Microdilution Method ◽

Resistant Strains ◽

Tract Infections

Antibiotics are becoming ineffective against resistant bacteria. The use of essential oils (EOs) may constitute an alternative solution to fight against multidrug-resistant bacteria. This study aims to determine the chemical composition of EOs from five populations of the endemic Algerian Origanum glandulosum Desf. and to investigate their potential antibacterial activity against multidrug-resistant uropathogenic E. coli strains. The EOs were obtained by hydrodistillation and their composition was investigated by gas chromatography/mass spectrometry (GC/MS). The antibacterial activity was evaluated by the disc diffusion method against eight E. coli strains (six uropathogenic resistant and two referenced susceptible strains). Minimum inhibitory and bactericidal concentrations (MIC/MBC) were obtained by the broth microdilution method. The main EO components were thymol (15.2–56.4%), carvacrol (2.8–59.6%), γ-terpinene (9.9–21.8%) and p-cymene (8.5–13.9%). The antibacterial tests showed that all the EOs were active against all the strains, including the multidrug-resistant strains. The EO from the Bordj location, which contained the highest amount of carvacrol (59.6%), showed the highest antibacterial activity (inhibition diameters from 12 to 24.5 mm at a dilution of 1/10). To our knowledge, this is the first description of the activity of O. glandulosum EOs against resistant uropathogenic strains. Our study suggests that O. glandulosum EO could be used in some clinical situations to treat or prevent infections (e.g., urinary tract infections) with multidrug-resistant strains.

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Antibacterial Activity and Chemical Composition of Red Peacock Flower (Caesalpinia pulcherrima L.) Leaf Essential Oil

Jurnal Kimia Sains dan Aplikasi ◽

10.14710/jksa.22.6.269-274 ◽

2019 ◽

Vol 22 (6) ◽

pp. 269-274

Author(s):

Novena Risnalani Rintank Constani ◽

Hartati Soetjipto ◽

Sri Hartini

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Inhibition Zone ◽

Diffusion Method ◽

Gram Positive ◽

Gram Negative ◽

E Coli ◽

Gram Positive Bacteria ◽

Leaf Essential Oil

Peacock flower (Caesalpinia pulcherrima L.) leaves contain essential oils which can be used as an ingredient in cosmetics, perfume, aromatherapy, medicine, and supplements. The study was conducted to obtain essential oils from peacock flower leaves and determine the antibacterial activity against gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) and gram-negative (Escherichia coli and Pseudomonas aeruginosa). Antibacterial activity test was carried out by the agar diffusion method, using paper discs. Measurements were made for the inhibition zone diameter (IZD) that appeared, while the essential oil component was analyzed using GC-MS. The results showed that the peacock flower leaves (C. pulcherrima) had a moderate to strong antibacterial effect at a concentration of 7.5%-20% against gram-positive bacteria (B. subtilis and S. aureus) and gram-negative bacteria (E. coli and P. aeruginosa). Gram-negative E. coli bacteria are relatively more sensitive to peacock flower leaf essential oil compared to other test bacteria. Peacock flower (C. pulcherrima) leaf essential oil is composed of 7 main components namely β-Cubebene 33.87%; Caryophyllene 23.00%; γ-Elemene 13.18%; α-Pinene 10.96%; Cadina-1(10),4-diene 10.20%; Copaene; 7.09%; β-Pinene 1.70%.

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Aktivitas Antioksidan dan Antimikroba Minyak Atsiri Kembang Leson

AL-Kauniyah Jurnal Biologi ◽

10.15408/kauniyah.v13i2.9966 ◽

2020 ◽

Vol 13 (2) ◽

pp. 139-146

Author(s):

Ambar Pratiwi ◽

Inas Salimah

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Inhibitory Concentration ◽

Herbal Medicines ◽

Diffusion Method ◽

E Coli ◽

Ic50 Value ◽

Dpph Method

AbstrakKembang leson adalah racikan jamu untuk mandi yang terdiri dari berbagai obat-obatan herbal yang umum ditemukan di Jawa. Kembang leson mengandung minyak atsiri utama, yaitu camphene 1,29%, benzene methyl cymene 4,93%, camphor 4,75%, cyclohexane methanol 7,56%, dan curdione 4,83%. Golongan senyawa minyak atsiri dapat menghambat radikal bebas 2,2-diphenyl-1-picrylhydrazyl (DPPH) dan memiliki aktivitas antibakteri yang ditunjukkan dengan uji aktivitas antibakteri terhadap Staphylococcus aureus dan Escherichia coli. Tujuan penelitian ini adalah untuk mengetahui aktivitas antioksidan dan antibakteri dari minyak atsiri kembang leson. Minyak Atsiri kembang leson diekstraksi dengan metode destilasi. Minyak atsiri yang diperoleh diuji aktivitas antioksidan dengan metode DPPH, serta aktivitas antibakteri dengan metode difusi. Hasil penelitian menunjukkan bahwa minyak atsiri kembang leson memiliki aktivitas antioksidan dengan persentase penghambatan DPPH sebesar 56,16%, dan memiliki nilai IC50 (half maximal inhibitory concentration) sebesar 825,78 ppm. Aktivitas antibakteri minyak atsiri kembang leson juga efektif menghambat pertumbuhan E. coli, tetapi belum mampu menghambat pertumbuhan S. aureus. Minyak atsiri kembang leson dapat digunakan sebagai sumber antioksidan alami. Abstract Kembang leson is an herbal concoction used for bathing. It comprises various herbal medicines found in Java. Kembang leson contains essential oils such as camphene 1.29%, benzene methyl cymene 4.93%, camphor 4.75%, cyclohexane methanol 7.56% and curdione 4.83%. It is known that essential oils can reduce DPPH free radicals and have antibacterial activity against S. aureus and E. coli. Our research to determine the antioxidant and antibacterial activity of kembang leson essential oil. The essential oil was extracted by distillation. The essential oil obtained was tested for antioxidant activity using the DPPH method, and antibacterial activity using the diffusion method. The results show that kembang leson essential oils have antioxidant and antibacterial activity. The percentage of DPPH inhibition was 56.16% and the IC50 value was 825.78 ppm. Antibacterial activity of kembang leson essential oil inhibited the growth of E. coli but could not inhibit the growth of S. aureus. Thus, kembang leson essential oils have antioxidant and antibacterial activity against E. coli, but only have antioxidant activity against S. aureus. Further studies are needed to determent the main ingredients that play an important role in the mechanism of antioxidants and antibacterial of kembang leson essential oil.

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Chemical Composition and Antibacterial Activity of Essential Oil of Melaleuca quinquenervia (Cav.) S.T. Blake (Myrtaceae)

Asian Journal of Applied Chemistry Research ◽

10.9734/ajacr/2020/v5i230132 ◽

2020 ◽

pp. 46-52

Author(s):

Alioune Diallo ◽

Yoro Tine ◽

Abdoulaye Diop ◽

Idrissa Ndoye ◽

Falaye Traoré ◽

...

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Diffusion Method ◽

Moderate Activity ◽

Melaleuca Quinquenervia ◽

E Coli ◽

Excellent Activity ◽

First Time

For the first time, this study aimed to determine the chemical composition and the antibacterial activity of Melaleuca quinquenervia leaf essential oils from Senegal. Ten samples of leaves from M. quinquenervia were collected on two Senegal localities. The essential oils were obtained by hydrodistillation and analyzed by GC/FID and GC/MS. The oil yields ofdried leaves ranged 1.65 to 3.74%. Oilsamples were dominated by 1,8-cineole (24.6-49.3%), viridiflorol (14.9-35.7%), a-terpineol (6.3-12.7%), a-pinene (5.0-11.5%) and limonene (3.7-7.3%). The antibacterial activity was evaluated using a paper disc diffusion method. The essential oil exhibited excellent activity against S. aureus, moderate activity against E. coli and E. faecalis and no activity against P. aeruginosa.

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Antibacterial activity of clove (Syzygium aromaticum) and cinnamon (Cinnamomum burmannii) essential oil against extended-spectrum β-lactamase-producing bacteria

Veterinary World ◽

10.14202/vetworld.2021.2206-2211 ◽

2021 ◽

pp. 2206-2211

Author(s):

Elgio Venanda Ginting ◽

Endah Retnaningrum ◽

Dyah Ayu Widiasih

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Morphological Changes ◽

Food Products ◽

Animal Origin ◽

Diffusion Method ◽

Syzygium Aromaticum ◽

E Coli ◽

Extended Spectrum

Background and Aim: Extended-spectrum β-lactamase (ESBL) is an enzyme produced by the family of Enterobacteriaceae, especially Escherichia coli and Klebsiella pneumoniae, which can hydrolyze β-lactam antibiotics, such as penicillins, cephalosporins, cephamycin, and carbapenem. ESBL-producing bacteria are widely distributed from farms to slaughterhouses until food products originating from animals are available in the market, which plays an important role as a pathway for the exposure and transmission of ESBL-producing bacteria from food products of animal origin to humans. This study aimed to determine the antibacterial activity of Syzygium aromaticum (clove) and Cinnamomum verum (cinnamon) essential oils against strains resistant to ESBL-producing E. coli and K. pneumoniae isolates. Materials and Methods: The antibacterial activity of clove and cinnamon essential oils was tested against three strains of tested bacteria using the disk diffusion method. The minimum inhibitory concentration (MIC) of clove and cinnamon essential oils was determined using the broth microdilution method. The minimum bactericidal concentration (MBC) was determined using the MIC. Morphological changes on each tested bacteria were observed through scanning electron microscopy (SEM). Results: Both essential oils exhibited inhibitory effects toward all test organisms, indicated by inhibition zones around the disk. The MIC values of clove essential oil were 0.078% (v/v) for all tested bacteria, whereas the MICs of cinnamon essential oil ranged from 0.039% (v/v) to 0.156% (v/v) for all tested bacteria. MBC values of clove and cinnamon essential oils ranged from 0.078% (v/v) to 0.156% (v/v) for all tested bacteria. There were morphological changes in each tested bacterial cell that was observed through SEM. Each tested bacteria treated with clove and cinnamon essential oils showed shrinkage and cells lysis. Conclusion: It was concluded that clove and cinnamon essential oils have emerged as effective antibacterial agents by showing high antibacterial activity against ESBL-producing E. coli and K. pneumoniae isolates, as evidenced by the inhibition zone diameter and MIC value.

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Chemical Composition, Antibacterial, Insecticidal and Anti-Oxidant Activities of Three Acantholimon Species (A. atropatanum, A. gilliatii and A. tragacanthium)

The Natural Products Journal ◽

10.2174/2210315509666190117153456 ◽

2020 ◽

Vol 10 (3) ◽

pp. 272-278

Author(s):

Ardalan Pasdaran ◽

Satyajit D. Sarker ◽

Lutfun Nahar ◽

Azadeh Hamedi

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Main Compound ◽

Aerial Parts ◽

Anti Oxidant ◽

Gas Chromatography Mass Spectroscopy

Background: The essential oil from the Acantholimon genus have been an integral part of the traditional food additive in Middle East. Most of the plants in Acantholimon genus have not been studied scientifically. The aim of this study is to investigate the chemical composition, antibacterial, insecticidal and anti-oxidant activities of three Acantholimon species including Acantholimon atropatanum, A. gilliatii and A. tragacanthium. Method: The essential oils of the aerial parts were extracted by hydrodistillation. Chemical constitutions were identified by gas chromatography- mass spectroscopy technique, also their toxicities were assessed against the two important grain products pests, Oryzeaphilus mercator and Tribolium castaneum. Antibacterial activity was assessed against the three foodborne bacteria that include Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus based on the disc diffusion assay. Free-radical-scavenging property was identified based on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Results: 2-hexahydrofarnesyl acetone was the main compound in A. gilliatii and A. tragacanthium, whilst farnesyl acetone, heptacosane and germacrene D were the principal components of A. atropatanum essential oil. These oils exhibited 40-90% mortality of O. mercator and/or T. castaneum at a dose of 12 μl/l air after 48h of exposure, and exhibited significant free-radicalscavenging property (RC50 = 3.7 × 10-3 - 8.3 × 10-3 mg/ml). The oils of A. tragacanthium and A. gilliatii showed a weaker antibacterial activity compared to A. atropatanum. Conclusion: A. atropatanum, A. gilliatii and A. tragacanthium essential oils had significant insecticidal and anti-oxidant properties. They also showed week to moderate antibacterial activity against P. aeruginosa and S. aureus.

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Evaluation of volatile constituents, exudation of resin and occurrence of galls of Protium aracouchini (Aubl.) Marchand

Acta Brasiliensis ◽

10.22571/2526-4338559 ◽

2021 ◽

Vol 5 (3) ◽

pp. 88

Author(s):

Thiago Augusto Araujo Correia Lima (in memorian) ◽

Leonardo Pinto Cunha ◽

José Eduardo Lahoz da Silva Ribeiro ◽

Marcia Ortiz Mayo Marques ◽

Maria da Paz Lima

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Chemical Constituents ◽

Brazilian Amazon ◽

High Concentration ◽

Forest Reserve ◽

Volatile Constituents ◽

Aerial Parts ◽

First Time ◽

Time Information

Protium aracouchini (Aubl.) Marchand [sin Icica aracouchini Aubl.], which occurs in the Adolpho Ducke Forest Reserve, in the Brazilian Amazon, was evaluated for the presence of galls, for resin exudation and the composition of the essential oils from the aerial parts and the resin. The experiment to stimulate the exudation of resin from the trunk was conducted using a 2-chloroethylphosphonic acid solution. The resin produced after 40 days and the aerial parts had their essential oils extracted in a Clevenger apparatus and the volatile chemical constituents were analyzed using GC/MS. The non-oxygenated sesquiterpenes α-copaene (21.15%) and α-gurjunene (13.69%), in addition to the oxygenated sesquiterpene spathulenol (10.32%), were detected as the majority constituents of the essential oil of the leaves, and a concentration similar to that of α-gurjunene was found in the branches (13.28%). The resin essential oil showed a high concentration of hydrocarbon monoterpenes (76.49%) with a predominance of α-pinene (17.57%) and limonene (46.11%). Four gall morphotypes were found associated with this species. The present study reports for the first time information on the volatile constituents and the resinous potential of P. aracouchini, and registers the morphotypes of the galls that help in the taxonomy of the species.

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Antibacterial activity of ginger extracts and its essential oil on some of pathogenic bacteria

Baghdad Science Journal ◽

10.21123/bsj.7.3.1159-1165 ◽

2010 ◽

Vol 7 (3) ◽

pp. 1159-1165

Author(s):

Baghdad Science Journal

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Essential Oil ◽

Pathogenic Bacteria ◽

Cold Water ◽

Water Extract ◽

Ethanolic Extract ◽

Hot Water ◽

Diffusion Method ◽

Resistant Bacteria

The antimicrobial activity of ginger extracts ( cold-water, hot-water, ethanolic and essential oil ) against some of pathogenic bacteria ( Escherichia coli , Salmonella sp , Klebsiella sp , Serratia marcescens, Vibrio cholerae , Staphylococcus aureus , Streptococcus sp) was investigated using Disc diffusion method , and the results were compared with the antimicrobial activity of 12 antibiotics on the same bacteria . The results showed that the ginger extracts were more effective on gram-positive bacteria than gram-negative . V. cholerae and S. marcescens,were the most resistant bacteria to the extracts used , while highest inhibition was noticed against Streptococcus sp (28 mm) . The ethanolic extract showed the broadest antibacterial activity ( 11 to 28 mm ) , in comparison with moderate activity of essential oil , it was observed that the cold-water extract was more effective on the bacteria than hot-water extract . Ginger ethanolic extract presented higher diameter of inhibition zone for Streptococcus sp than in Ciprofloxacin , Cefotaxime , Cefalotin , Cephalexin and Cephaloridine , also it was found a similarity between the higher inhibition zones of ethanolic extract of ginger and some antibiotics for S. aureus , E. coli , Salmonella sp and Klebsiella sp . V. cholerae and S. marcescens,also highly resistant to antibiotics . Phytochemical analysis of ethanolic extract of ginger revealed the present of glycosides, terpenoids, flavonids and phenolic compounds

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Chemical Composition and Antibacterial Activity of Essential Oil of Anvillea radiata Coss. & Dur

Natural Product Communications ◽

10.1177/1934578x0700200517 ◽

2007 ◽

Vol 2 (5) ◽

pp. 1934578X0700200

Author(s):

Fadwa El Hanbali ◽

Ahmed El Hakmaoui ◽

Fouad Mellouki ◽

Lahoussine El Rhaffari ◽

Mohamed Akssira

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Aerial Parts

The essential oil of the aerial parts of Anvillea radiata Coss. & Dur. was examined by GC-MS. Twenty-nine constituents were identified, representing 88.8% of the total. 6-Oxocyclonerolidol (66.6%) and 6-hydroxycyclonerolidol (11.4%) were found to be the major components. The disc diffusion method, used for antimicrobial activity testing, showed that the essential oil had a significant antibacterial activity against all tested microorganisms.

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