scholarly journals Gambaran Hasil Aktivitas Antioksidan Berberapa Perlakuan Teknik Preparasi Pembuatan Konsentrasi Ekstrak Kulit Buah Nanas (Ananas comosus L.Merr) Menggunakan Senyawa DPPH (1,1-diphenyl-2-picrylhydrazyl)

2019 ◽  
Vol 10 ◽  
pp. 44-47
Author(s):  
Syarifah maryam Alaydrus ◽  
Wahyu Widayat ◽  
Laode Rijai
Keyword(s):  
Antioxidant Activity ◽  
Volume Ratio ◽  
Ananas Comosus ◽  
Preparation Process ◽  
Extract Concentration ◽  
Fruit Skin ◽  
Pineapple Peel ◽  
Pineapple Fruit ◽  
Preparation Techniques ◽  
Peel Extract

Pineapple skin (Ananas comosus L.Merr) has been known to have a category of strong antioxidant activity against reducing DPPH compounds (1,1-diphenyl-2-picrylhydrazyl). However, references to the preparation process of extract concentration making and the treatment of DPPH compounds to extracts are not much elaborated, so that it can cause errors in inferring the category of antioxidant activity. The purpose of this study was to determine the results of antioxidant activity through the treatment of preparation techniques for the concentration of pineapple peel extract concentration. Pineapple peel extract (Ananas comosus L. Merr) was prepared by maceration method using 70% ethanol solvent. Testing of antioxidant activity was carried out using the UV-Vis spectrophotometric method at a wavelength of 516.4 nm. The extract concentration to be tested was 500 μg/mL, 250 μg/mL, 125 μg/mL, 62.5 μg/mL and 31.25 μg/mL and the concentration of DPPH compounds (1,1-diphenyl-2-picrylhydrazyl) was 45 μg/mL. Treat the extract making concentration divided into 3 namely P1 (multilevel dilution with a volume ratio of 1: 1) P2 (normal dilution with a volume ratio of 1: 1) and P3 (DPPH solution is used to suffice the volume of the extracted test extract). The results of percent inhibitors from each treatment of making concentrations showed P1 <P2, P1 <P3, P2 <P3, and IC50 of each treatment were P1 268.07 μg/mL, P2 was 231.28 μg/mL, P3 was 78.403 μg/mL. Based on the results of IC50 P3 gives the category of antioxidant activity that is 78,403.Keywords: pineapple fruit skin (Ananas comosus L. Merr), antioxidants, dpph (1,1-diphenyl-2- picrylhydrazyl), dilution.

scholarly journals EVALUASI KARAKTERISTIK FISIKA-KIMIA SEDIAAN KRIM DAN LOTION EKSTRAK KULIT BUAH NANAS (Ananas comosus L.Merr)

2018 ◽  
Vol 15 (2) ◽  
pp. 41
Author(s):  
Elya Zulfa ◽  
Mufrod Mufrod
Keyword(s):  
Physical Chemistry ◽  
Ethanolic Extract ◽  
Ananas Comosus ◽  
Chemical Characteristics ◽  
Physical Chemical ◽  
Fruit Skin ◽  
Pineapple Peel ◽  
Pineapple Fruit ◽  
Positive Controls ◽  
Made In

ABSTRACTIn previous research, ethanolic extract of pineapple peel (EEPP) has activity as a sunscreen because of the content of flavonoids. EEPP can be formulated in the form of creams and lotions to facilitate use on the skin. This study aims to evaluate the physical chemistry characteristics of EEPP creams and lotions. Creams and lotions were made in 3 formulas based on variations in FI extract concentrations (15.0%), FII (17.5%), FIII (20.0%), base controls and positive controls (preparations on the market). The cream obtained was evaluated for physical chemical characteristics including organoleptic, homogeneity, adhesion and dispersion, viscosity, pH and SPF values which were analyzed descriptively. cream with varying concentrations of green, the distinctive smell of pineapple, homogeneous and in accordance with the pH of the skin. Increasing the concentration of EEPP in the preparation has an effect on increasing viscosity, adhesion but decreasing the dispersion. Whereas the lotion shows greenish color, aromatic odor and semisolid texture and is easily applied. The increase in EEPP in lotion affects the increase in pH, adhesion, but decreasing the dispersion.Keywords: Creams, lotions, sunscreen, pineapple fruit skin

scholarly journals Antibacterial Activity Test of Ethanol Extract Pineapple (Ananas comosus (L.) Merr.) Peel against Growth of Propionibacterium acnes

2019 ◽  
Vol 2 (2) ◽  
pp. 108-113
Author(s):  
Fitriyanti Fitriyanti ◽  
Muhammad Nur Rahman Hendrawan ◽  
Karunita Ika Astuti
Keyword(s):  
Antibacterial Activity ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Ananas Comosus ◽  
Treatment Groups ◽  
Pineapple Peel ◽  
Pineapple Fruit ◽  
Peel Extract

Pineapple peel (Ananas comosus (L.) Merr.) is waste from the pineapple fruit. The purpose of this study was to determine the content of pineapple peel extract and to antibacterial activity in various concentrations effective in inhibiting the growth of Propionibacterium acnes. The pineapple peel extract is made by using the extraction method in the form of maceration. The method used in the inhibitory test using the three replication samples in each treatment group. The sample consisted of 10 treatment groups ie pineapple peel extract concentration 12.5%, 25%, 37.5%, 50%, 62.5%, 75%, 87.5%, and 100%, as well as positive and negative control. The results obtained from pineapple peel screening contain flavonoid and saponin compounds. The data analysis shows that pineapple peel extract concentrations of 50%, 62.5%, 75%, 87.5%, and 100% had inhibitory zones with the medium-strong category while clindamycin as the control has a strong inhibition zone. The conclusion of this research proves that pineapple peel extract has antibacterial power to P. acnes with effective concentration is 100% with the strong category.

scholarly journals Antibacterial activity of mixed pineapple peel (Ananas comosus) extract and calcium hydroxide paste against Enterococcus faecalis

2018 ◽  
Vol 51 (1) ◽  
pp. 20
Author(s):  
Intan Fajrin Arsyada ◽  
Devi Rianti ◽  
Elly Munadziroh
Keyword(s):  
Antibacterial Activity ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antibacterial Properties ◽  
Root Canal Treatment ◽  
Ananas Comosus ◽  
Treatment Groups ◽  
Pineapple Peel ◽  
Peel Extract

Background: Enterococcus faecalis (E. faecalis) is the bacteria most commonly resulting from failed root canal treatment. Intracanal medicament is used to enhance the success of root canal treatment. A material widely used for this purpose is calcium hydroxide. However, its ineffectiveness in eliminating E. faecalis requires the addition of other antibacterial substances, such as iodoform which has the disadvantage of having toxic effects on tissues. Pineapple peel has antibacterial properties because it contains chemical compounds, such as flavonoid, saponin, tannin, as well as the enzyme bromelain. Purpose: The aim of the study was to determine the antibacterial activity of a mixture of pineapple peel extract at 6.25% and 12.5% concentrations and calcium hydroxide paste at a ratio of 1:1 compared to100% calcium hydroxide and a mixture of calcium hydroxide and iodoform paste against E. faecalis. Methods: The research was laboratory-based experiment in nature. Sample groups were divided into two control groups (one featuring100% calcium hydroxide paste and a second featuring a mixture of calcium hydroxide and iodoform paste) and two treatment groups (mixture of pineapple peel extract and calcium hydroxide paste in 6.25% and 12.5% concentrations with ratio 1:1). The method was using agar diffusion. The result data were analyzed by One Way Anova test. Results: The highest average of the inhibitory zone occurred in group with a mixture of pineapple peel extract 12.5% and calcium hydroxide paste while the smallest average was that of group with a mixture of calcium hydroxide and iodoform paste. Conclusion: Mixture of pineapple peel extract in 6.25% and 12.5% concentrations and calcium hydroxide paste ratio 1:1 has higher antibacterial activity than paste of 100% calcium hydroxide and mixture of calcium hydroxide and iodoform paste againts E. faecalis.

scholarly journals ANTIOXIDANT POTENTIAL OF DIFFERENT PARTS OF BOGOR PINEAPPLE (ANANAS COMOSUS [L.] MERR. VAR. QUEEN) CULTIVATED IN WEST JAVA-INDONESIA

2018 ◽  
Vol 11 (1) ◽  
pp. 129
Author(s):  
Irda Fidrianny ◽  
Veliana Virna ◽  
Muhamad Insanu
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Ananas Comosus ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Antioxidant Power ◽  
Pineapple Peel ◽  
Dpph Method ◽  
Peel Extract ◽  
Different Parts

  Objective: The aims of this research were to observe antioxidant activities from different parts of Bogor pineapple (Ananas comosus [L.] Merr. Var. Queen) using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) and correlation of total phenolic and flavonoid contents with their inhibitory concentration 50% (IC50) of DPPH and exhibitory concentration 50% (EC50) of FRAP.Methods: Each sample was extracted by reflux using different polarity solvents. Antioxidant activities were determined using DPPH and FRAP assays, total phenolic content (TPC) using Folin–Ciocalteu reagent, flavonoid content by Chang’s method, and correlation with their IC50 DPPH and EC50 FRAP were analyzed by Pearson’s method.Results: IC50 DPPH of various extracts of different parts of Bogor pineapple ranged from 0.13 to 68.17μg/ml. The ethyl acetate peel extract of Bogor pineapple presented the highest TPC (7.84 g GAE/100 g) while the highest total flavonoid content (10.84 g QE/100 g) was shown by ethyl acetate bract extract of Bogor pineapple. TPC in peel extract of Bogor pineapple had negative and significant correlation with their EC50 FRAP. The IC50 DPPH and EC50 FRAP of peel extract of Bogor pineapple showed positive and significant correlation.Conclusion: All different part extracts of Bogor pineapple (except n-hexane flesh extract, peel extract, and bract extract) were categorized as a very strong antioxidant by DPPH method. Phenolic compounds in peel extract of Bogor pineapple were the major contributor in antioxidant activities by FRAP method. DPPH and FRAP methods gave linear results in antioxidant activities of Bogor pineapple peel extract.

scholarly journals DAYA ANTI BAKTERI EKSTRAK KULIT NANAS (Ananas comosus) TERHADAP PERTUMBUHAN BAKTERI Enterococcus faecalis ANTIBACTERIAL POTENCY OF PINEAPPLE PEEL EXTRACT (Ananas comosus) ON Enterococcus faecalis GROWTH

2016 ◽  
Vol 6 (2) ◽  
pp. 61
Author(s):  
Rega Maurischa Arantika Putri ◽  
Tamara Yuanita ◽  
Mohammad Roelianto
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Positive Control ◽  
Ananas Comosus ◽  
Control Group ◽  
Control Group Design ◽  
Antibacterial Drugs ◽  
Bacteria Growth ◽  
Pineapple Peel ◽  
Peel Extract

Background: Root canal infections is caused by the colonization of microorganisms. Microorganisms that commonly found in root canal failure is Enterococcus faecalis. These bacteria can be found in 80-90% of root canal infections. Currently, many plants are used as antibacterial drugs. Pineapple is one plant that can be used as antibacterial drugs. Pineapple peel has a variety of antibacterial chemical compounds, such as, bromelain, saponins, tannins and flavonoids that can inhibit the growth of Enterococcus faecalis bacteria. Aim: To obtain concentration of antibacterial from pineapple peel extract on Enterococcus faecalis growth. Methods: This study was an experimental laboratory with Post Test Only control group design using Enterococcus faecalis ATCC 29212 bacteria that were diluted based on Mc. Farland standard 1.5 x 108 CFU / ml with pineapple peel extract treatment concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0, 78% and then planted in nutrient agar media surface evenly. Results: At concentration of 3.125% pineapple peel extract showed that the growth of colonies was less than 90% of positive control and concentration of 6.25% pineapple peel extract had no visible Enterococcus faecalis bacteria growth as much as 99.9%. Conclusion: The pineapple peel extract (Ananas comosus) have antibacterial power on the growth of Enterococcus faecalis bacterial colonies with Minimal Inhibitory Concentration (MIC) of 3.125% and the Minimum Bactericidal Concentration (MBC) of 6.25%.

scholarly journals UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK KULIT NANAS (Ananas comosus. L) TERHADAP PERTUMBUHAN Ralstonia solanacearum

2018 ◽  
Vol 2 (2) ◽  
pp. 109-114
Author(s):  
Astherix Putri Yudha ◽  
Ngadiani Ngadiani
Keyword(s):  
Ralstonia Solanacearum ◽  
Agar Medium ◽  
Positive Control ◽  
Ananas Comosus ◽  
Tetrazolium Chloride ◽  
Pineapple Peel ◽  
Number Of Bacteria ◽  
Peel Extract

This study aims to determine the effectiveness of antibacterial pineapple peel extract (Ananascomosus. L) on Ralstonia solanacearum growth. Pineapple peel extract (Ananas comosus.L) containsantibacterial compounds of bromelin and tannin enzymes that are able to inhibit the growth of plantcausing bacteria, Ralstonia solanacearum. Ralstonia solanacearum bacteria will be given 5treatments with pineapple peel extract 10%, 30%, 60%, 100% and positive control Streptomicinsulfate 0.01%, each repeated 5 times. The test was performed by adding 100μl of Ralstoniasolanacearum suspension which was diluted into 15ml Tetrazolium chloride agar medium which wasgiven 100 ml of pineapple peel extract. The results showed that the treatment of pineapple peel extracthad a significant effect on the growth of Ralstonia solanacearum. The average number of treatedbacteria 60% (4x107 CFU), 100% (8x106 CFU) and Streptomicin sulfate 0.01% (4x106 CFU) wassignificant (P <0.01) higher than the number of bacteria treated by concentration 10% (1.79x108)and 30% (1.27x108). Consentration 100% pineapple peel extract (Ananas comosus.L) is the mosteffective in inhibiting the growth of Ralstonia solanacearum. Thus it can be concluded that pineapplepeel extract (Ananas comosus.L) is effective as an antibacterial Ralstonia solanacearum.

scholarly journals SIFAT ORGANOLEPTIK DAGING AYAM PETELUR TUA YANG DIRENDAM DALAM EKSTRAK KULIT NANAS (Ananas comosus L. Merr)

ZOOTEC ◽  
2017 ◽  
Vol 37 (2) ◽  
pp. 303
Author(s):  
Ahmad Dewanto ◽  
M. D. Rotinsulu ◽  
T. A. Ransaleleh ◽  
R. M. Tinangon
Keyword(s):  
Analysis Of Variance ◽  
Ananas Comosus ◽  
Test Measurement ◽  
Organoleptic Test ◽  
Randomized Design ◽  
Pineapple Peel ◽  
Completely Randomized Design ◽  
Materials Used ◽  
Hedonic Scale ◽  
Peel Extract

The objective of this study was to evaluate the organoleptic characteristic of old laying meat immersed in pineapple peel extract (Ananas comusus L. Merr). The materials used in this study were 25 birds of the age of 24 months old and the extract of 10 pineapple peels. The Completely Randomized Design was applied in this study, involving the organoleptic test measurement with hedonic scale and untrained panelist of 40 peoples. Treatments were involving immersion of old laying meat in pineapple peel extract with levels:R0 = Without immersion in pineapple peel extract; R1 = Immersion in pineapple peel extract during 1 hour; R2 = Immersion in pineapple peel extract during 2 hours; R3 = Immersion in pineapple peel extract during 3 hours; R4 = Immersion in pineapple peel extract during 4 hours. Analysis of variance were used for organoleptic test and showed that treatments affected significantly (P<0.01) the colour, flavour, tenderness, texture, and taste of ald laying meat. Study indicated that panelists preferred the texture and tenderness of laying meat immersed during one hour in pineapple extract however they preferred the meat colour and taste without immersion.Keywords: Laying meat, pineapple peel, organoleptic

Functionalization of Wool Fabric Using Pineapple Peel Extract (PPE) as a Natural Dye

2019 ◽  
Vol 6 (5) ◽  
pp. 16-20
Author(s):  
Javed Sheikh ◽  
Ashutosh Agrawal ◽  
Harsh Garg ◽  
Aashi Agarwal ◽  
Prasun Mathur
Keyword(s):  
Antioxidant Activity ◽  
Functional Properties ◽  
Wool Fabric ◽  
Fastness Properties ◽  
Color Strength ◽  
Wool Fabrics ◽  
Textile Functionalization ◽  
Pineapple Peel ◽  
Dyed Fabrics ◽  
Peel Extract

Large quantities of pineapple peel waste are discarded every year that can be used for extraction of useful textile functionalization chemicals. In the present work, pineapple peel extract (PPE) was used for simultaneous dyeing and multifunctional finishing of wool fabric. The PPE was used for dyeing wool fabrics, with and without mordants. The dyed fabrics were characterized and evaluated for color values and fastness properties. The functional properties of the dyed fabrics viz. antibacterial activity, UV protection, and antioxidant activity were evaluated. It was confirmed that wool fabric can be dyed using pineapple peel extract without mordants; however, additional color strength can be gained using pre-mordanting. The dyed fabrics showed light shades with good fastness properties along with excellent functional properties.

scholarly journals ANTIOXIDANT POTENTIAL OF DIFFERENT PARTS OF BOGOR PINEAPPLE (ANANAS COMOSUS [L.] MERR. VAR. QUEEN) CULTIVATED IN WEST JAVA-INDONESIA

2018 ◽  
Vol 11 (1) ◽  
pp. 129
Author(s):  
Irda Fidrianny ◽  
Veliana Virna ◽  
Muhamad Insanu
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Ananas Comosus ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Antioxidant Power ◽  
Pineapple Peel ◽  
Dpph Method ◽  
Peel Extract ◽  
Different Parts

  Objective: The aims of this research were to observe antioxidant activities from different parts of Bogor pineapple (Ananas comosus [L.] Merr. Var. Queen) using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) and correlation of total phenolic and flavonoid contents with their inhibitory concentration 50% (IC50) of DPPH and exhibitory concentration 50% (EC50) of FRAP.Methods: Each sample was extracted by reflux using different polarity solvents. Antioxidant activities were determined using DPPH and FRAP assays, total phenolic content (TPC) using Folin–Ciocalteu reagent, flavonoid content by Chang’s method, and correlation with their IC50 DPPH and EC50 FRAP were analyzed by Pearson’s method.Results: IC50 DPPH of various extracts of different parts of Bogor pineapple ranged from 0.13 to 68.17μg/ml. The ethyl acetate peel extract of Bogor pineapple presented the highest TPC (7.84 g GAE/100 g) while the highest total flavonoid content (10.84 g QE/100 g) was shown by ethyl acetate bract extract of Bogor pineapple. TPC in peel extract of Bogor pineapple had negative and significant correlation with their EC50 FRAP. The IC50 DPPH and EC50 FRAP of peel extract of Bogor pineapple showed positive and significant correlation.Conclusion: All different part extracts of Bogor pineapple (except n-hexane flesh extract, peel extract, and bract extract) were categorized as a very strong antioxidant by DPPH method. Phenolic compounds in peel extract of Bogor pineapple were the major contributor in antioxidant activities by FRAP method. DPPH and FRAP methods gave linear results in antioxidant activities of Bogor pineapple peel extract.

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