scholarly journals Perbandingan Reaksi Zat Besi Terhadap Teh Hitam dan Teh Hijau Secara In Vitro dengan Menggunakan Spektrofotometer Uv-Vis

2014 ◽  
Vol 3 (1) ◽  
Author(s):  
Husnil Wardiyah ◽  
Yustini Alioes ◽  
Dian Pertiwi

AbstrakSalah satu permasalahan gizi yang dihadapi Indonesia adalah anemia defisiensi besi. Defesiensi besi ini dapat disebabkan oleh asupan dan serapan yang tidak adekuat, seperti kebiasaan mengonsumsi zat yang dapat menghambat penyerapan zat besi seperti minum teh pada saat makan. Hambatan penyerapan ini disebabkan oleh polifenol yang terkandung di dalam teh, terutama tanin. Penelitian ini dilakukan pada teh hitam dan teh hijau yang banyak dikonsumsi masyarakat. Larutan teh hitam dan teh hijau dijadikan sebagai kontrol, kemudian diberikan perlakuan dengan meneteskan FeCl3 1% sebanyak lima tetes. Larutan tersebut dibaca besar absorbannya dengan spektrofotometer UV-Vis. Prosedur ini dilakukan dengan pengulangan sebanyak lima kali. Data hasil penelitian diolah dengan menggunakan independent sample t test untuk melihat perbedaan rata-rata pada dua kelompok sampel tersebut. Dari hasil penelitian didapatkan rata-rata besar absorban teh hitam kontrol 0,539 dan setelah diteteskan zat besi 0,30640. Absorban teh hijau kontrol 0,961 dan setelah diteteskan zat besi 0,65020. Hal ini berarti bahwa terjadi penurunan konsentrasi larutan tersebut. Penurunan absorban pada kontrol teh hitam dengan perlakuan adalah 43,15%, sedangkan pada teh hijau adalah 32,34%. Berdasarkan uji statistik, disimpulkan bahwa terdapat perbedaan bermakna antara absorbansi teh hitam dan teh hijau.Kata kunci: Teh hitam, teh hijau, zat besi, absorban.AbstractOne of the nutritional problems faced by Indonesia is iron deficiency anemia. It is caused by inadequate intake and absorption. One of the causes of this inadequate absorption is eating habit to consume substances that can inhibit iron absorption like drinking tea while eating. This is caused by tea polyphenol compounds, especially tannins. Black tea and green tea were observed in this research since these are widely consumed by public. Solution of black tea and green tea were used as control and they were treated by giving five drops of 1% FeCl3. Absorbance of those solutions was read by using spectrophotometer UV-Vis. This procedure was done with five times repetition. The result of this research was processed by using independent sample t test to discern mean of two groups samples. The result showed that the absorbance mean of black tea control is 0.539 and the absorbance mean of black tea that has dripped by iron is 0.30640. As for the green tea control absorbance is 0.961 and absorbance mean after iron sheds is 0.65020. It means that there is a dilution and reduction of solution concentration. Based on these figures, it was concluded that the concentration of black tea with iron sheds is smaller than the concentration of green tea with iron sheds. Nevertheless, there is a greater reduction in black tea compare with green tea in the absorbance reduction. Absorbance reduction from black tea control group to treatment group is 43.15%, while absorbance reduction from green tea control group to treatment group is 32.34%. Based on statistical tests, it was concluded that there is a significant difference between the absorbance of black tea and green tea.Keywords: Black tea, green tea, iron, absorbance.

2021 ◽  
Vol 3 (2) ◽  
pp. 71-85
Author(s):  
arista wahyu ningsih ◽  
◽  
Nur Arzy Samsiyeh ◽  
Martina Kurnia Rohmah ◽  
◽  
...  

Diabetes mellitus is a disease or metabolic disorder that is most often found in society, including one of the diseases or metabolic disorders. Tamarind leaves (Tamarindus indica L) is a plant that can be used as an alternative treatment for Diabetes Mellitus which contains flavonoids, sapoin and tannins, which can lower glucose levels and inhibit glucose absorption in the blood. This study aims to determine the differences in blood sugar levels before and after giving tamarind leaves infusion in the village community of Tanjung Bumi. This type of research is Quasy Experiment Design and purposive sampling technique with a sample of 29 respondents. Based on the results of the paired t-test statistical analysis showed a significant difference (p = 0.000) between the blood sugar levels of the group and placebo. Meanwhile, in the posttest the treatment group after giving tamarind leaves showed the same as the control group, namely p = 0.000, which means that there is also a difference in the decrease in blood sugar levels between the sebelum pemberian perlakuan and posttest. The results of statistical tests with the Independent T-Test showed the results in both groups with a value of p = 0.000, meaning that there was a difference in the decrease in blood sugar levels between the control group and the treatment group. Steeping tamarind leaves has an effect in reducing blood sugar levels in the body


2007 ◽  
Vol 19 (1) ◽  
Author(s):  
Isti Endah Kurniwati ◽  
Juni Handajani ◽  
Regina TC. Tandelilin

Green tea (Camellia sinensis) has been widely known as a healthy drink since long time ago. One of the substances in green tea which can give health benefit is catechin, an antibacterial substance. The purpose of this research is to know the efficacy of rinsing with green tea extract to Streptococcus alpha growth on gingivitis patient’s dental plaque. The research subjects include 30 mild gingivitis patients, and these subjects are divided into two groups: treatment group (20 patients) and control group (10 patients). Ten patients of treatment group rinse their mouth with 0.25% green tea extract and 10 other patients with 0.5% green tea extract. Meanwhile, control group use Bactidol (0.1% Hexetidine). Rinsing the mouth is performed every morning and night for five days. Sampling is conducted on the first and sixth day. Before rinsing data is analyzed using ANOVA and the result shows a significant difference. ANAVA testing then is done using proportion value. ANAVA Testing result shows that there is no significant difference among the patients in the treatment group. This result indicates that the effect of rinsing with 0.25% and 0.5% green tea extract is equivalent to the control (0.1% Hexetidine) to inhibit S. alpha growth on mild gingivitis patient’s dental plaque. From these two concentrations, it has not been known which one is the most effective concentration to inhibit S. alpha growth on mild gingivitis patient.


2019 ◽  
Vol 7 (6) ◽  
pp. 1032-1036 ◽  
Author(s):  
I Gusti Agung Ayu Dharmawati ◽  
Tjokorda Gde Bagus Mahadewa ◽  
I Putu Eka Widyadharma

AIM: The purpose of this study was to determine the antibacterial activity of Lumbricus rubellus earthworms through inhibitory zone diameter to the growth of the bacterium Phorphyromonas gingivalis as the cause of periodontitis. METHODS: This was an experimental study with randomised posttest-only control group design. The study was conducted at the Microbiology Research Center laboratory at the Faculty of Dentistry, Airlangga University, Indonesia. The study was conducted in vitro, the sample size was calculated using the Federer formula as many as four agar plates containing bacteria Phorphyromonas gingivalis, with each plate given five different treatments: control (ethanol), Lumbricus rubellus earthworm extract (ECT) with concentrations of 50%, 25%, 12.5%, and 6.25% respectively. The data in the form of inhibition zone diameter (measured in millimetres) obtained were tested using One-Way ANOVA. RESULTS: The mean diameter of the inhibitory zone extract of Lumbricus rubellus earthworm on the growth of Phorphyromonas gingivalis bacteria in the treatment group had significant differences (p < 0.05). The mean inhibition zones between controls and the ECT treatment group (ECT 50%, ECT 25%, ECT 12.5%) were statistically different (p < 0.05), in contrast with ECT 6.25% (p > 0.05) which did not show significant difference with the control group (p > 0.05). CONCLUSION: Lumbricus rubellus earthworm extract with a concentration of 50% has the largest diameter of the inhibitory zone on the growth of the Phorphyromonas gingivalis bacteria. The 6.25% earthworm extract showed no antibacterial activity against the growth of Phorphyromonas gingivalis bacteria.


2018 ◽  
Vol 4 (2) ◽  
pp. 83-90
Author(s):  
I Gusti Agung Tresna Wicaksana ◽  
Teguh Wahju S ◽  
Rinik Eko K ◽  
Putu Yudara S

Background: Panti werdha in Indonesia is considered as a nursing home for elderly. However, there are still psychosocial problems occur such as loneliness, sadness, and anxiety disorders. Acceptance and Commitment Therapy (ACT) may be able to solve psychosocial problems especially for anxiety.Objective: To analyze the extent of ACT's influence on the level of anxiety of the elderly living in the PSTW Bali.Methods: This study was a quasi-experimental study with pretest posttest with control group. Sixty elderly were recruited and divided into control and treatment groups with 30 samples of each group. Paired and Independent T-test were used for data analysis.Results: The paired T-test results in the control group showed significant differences of anxiety score between pretest and posttest after given generalist therapy (p 0.05). The result of paired T-test in the treatment group showed that there was a significant difference of anxiety score between pretest and posttest after given general and ACT therapy (p 0.05). The independent T-test showed that there was a significant difference of anxiety score between control group and treatment group (p 0.05).Conclusion: ACT psychotherapy is effective in decreasing anxiety in elderly who are living in PSTW Bali. 


2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Desi Sarli ◽  
Masrul Masrul ◽  
Meilinda Agus

Abstrak Upaya penanganan perdarahan postpartum adalah dengan pemberian oksitosin yang mempunyai peranan penting dalam merangsang kontraksi otot polos uterus sehingga perdarahan dapat teratasi.Hormon oksitosin dapat dihasilkan melalui rangsangan pemijatan oksitosin yang akan mempercepat kerja saraf parasimpatis untuk menyampaikan perintah ke hipotalamus untuk menghasilkan oksitosin. Tujuan penelitian ini adalah untuk mengetahui pengaruh perbedaan kadar oksitosin melalui pemijatan oksitosin terhadap jumlah perdarahan pada ibu 2 jam postpartum. Penelitian menggunakan desain eksperimen. Penelitian ini dilaksanakan ± 6 bulan dengan jumlah sampel 64 orang. Pengolahan data dilakukan dengan komputerisasi. Data disajikan dalam bentuk distribusi frekuensi dan selanjutnya dilakukan uji independen t-test, uji korelasi dan regresi untuk mengetahui pengaruh hubungan kedua variabel. Hasil penelitian perbedaan kadar oksitosin pada ibu 2 jam postpartum pada kelompok intervensi mempunyai rata-rata kadar oksitosin 47.16 pg/ml dengan standar deviasi 17.583 pg/ml,sedangkan kadar oksitosin  pada kelompokkontrol 29.86 pg/ml dengan standar deviasi 17.532 pg/ml dengan nilai p<0,05.Rata-rata jumlah perdarahan pada kelompok intervensi 175.00 ml dengan standar deviasi 48.894 ml,sedangkan jumlah perdarahan  pada kelompok kontrol 247.06 ml dengan standar deviasi 72.093 ml dengan nilai p<0,05. Hasil uji korelasi didapatkan hubungan kadaroksitosin terhadap jumlah perdarahan menunjukkan hubungan sedang (r=0,482). Hasil uji statistik didapatkan ada perbedaan kadar oksitosin terhadap jumlah perdarahan (p<0,05). Terdapat perbedaan yang bermakna antara kadar oksitosin ibu 2 jam postpartum pada kelompok intervensi dan kelompok kontrol.Terdapat perbedaan yang bermaknaantara jumlah perdarahan ibu 2 jam postpartum pada kelompok intervensi dan kelompok kontrol. Semakin tinggi kadar oksitosin maka jumlah perdarahan semakin sedikit.Kata kunci: Pemijatan oksitosin, oksitosin, jumlah perdarahan 2 jam postpartumAbstract Efforts to handling postpartum hemorrhage is to give oxytocin,which it is an important role in oxytocin stimulates uterine smooth muscle contraction, so that bleeding can be resolved.The hormone oxytocin can be generated through the stimulation of oxytocin massage that will accelerate parasympathetic nerves to deliver commands to the hipotalamus to produce oxytocin. The objective of this study was to determine effect of different levels of oxytocin trough massage of oxytocin on the amount of bleeding at 2 hours postpartum. This research use experimental designthat was conducted ± 6 months to 64 people. Data processing was done by computerized. The data presented in the form of a frequency distribution and performed an independen t-test and correlation test and regression to determine the effect ofthe relationship between the two variables. There is differences the levels of oxytocin at 2 hours postpartum in the intervention group had higher median levels of oxytocin 47.16pg/ml with a standard deviation of 17.583pg/ml, whereas the levels ofoxytocin at 2 hours post partum control group 29.86 pg/ml with a standard deviation of 17.532 pg/ml with p<0.05. The average of bleeding in the intervention group was 175.00 ml with a standard deviation of 48.894 ml, while the amount of bleeding at 2 hours postpartum control group 247.06 ml with a standard deviation of 72.093 ml with p<0.05. The results obtained correlation levels of oxytocin relation to 2 hours postpartum hemorrhage showed moderate relationship (r =0.482). The results of statistical tests found differences in the levels of oxytocin on the amount of bleeding at 2 hours postpartum (p<0.05). There is differences between the levels of oxytocin 2 hours pos partum in the intervention group and the group control. There are significant difference between the hemorrhage 2 hours post partum in the intervention group and the group of high levels of oxytocin control. Keywords: massage of oxytocin, oxytocin, amount of bleeding at 2 hours postpartum


2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Maryam Abiz ◽  
Hasan Robabi ◽  
Alireza Salar ◽  
Farshid Saeedinezhad

Background: Tuberculosis (TB) affects various aspects of quality of life (QoL), and self-care is the most important form of primary care and one of the main factors involved in the process of treating chronic diseases and improving the patients’ QoL. Objectives: This study aimed to investigate the effect of self-care education on the QoL of TB patients. Methods: In this quasi-experimental study, 110 TB patients referred to the TB coordinating Center in Zahedan, Southeastern Iran, in 2018, are studied. Participants were selected using convenience sampling and were randomly assigned to the intervention (n = 55) and control (n = 55) groups. Data were collected using the tuberculosis quality of life-version 2 (TBQol-v2). For the intervention group, first, patients and their caregivers were divided into groups compromising of 4 to 6 members, and then three sessions of self-care education, including lectures and questions and answers meetings, each lasted for 30 minutes (in total 90 minutes) were provided to each group. Data were analyzed in SPSS version 21 using descriptive statistical tests, independent t-test, paired t-test, and chi-squared test. Results: Mean of the total QoL score of the two groups before the intervention was 67.56 ± 5.99 and 67.09 ± 5.03, respectively, indicating no significant difference (P = 0.65). But after providing the intervention, the mean score of patients QoL in the intervention group (74.84 ± 4.90) was significantly higher than the control group (67.98 ± 0.68) (P = 0.001). Conclusions: Since self-care education can effectively enhance the QoL of TB patients, it is recommended to provide such educations for both treatment and follow-up of these patients along with directly observed treatment, short-course (DOTS).


Author(s):  
Ning Setianti

The learning have been tended to put more emphasis on knowledge transfer. One of the efforts in improving students' knowledge about the concepts of ecosystem and relation to students’ environmental behavior is integrating Geography and environmental education developing instructional packages based on ecosystem for teachers of Geography. This study aims to develop an instructional packages geography based on ecosystem (PIGeoEk) in improving students' knowledge of the concepts of the ecosystem and related to students’ environmental behavior. A research and development (R & D) based on Borg and Gall (2007), refering to the Dick and Carey design (2009), steps has been applied by involving 80 students of grade XI majoring in social sciences of SMAN 5 Depok for treatment, and grade XI majoring in social sciences of SMAN 3 Depok as a control group. There are four instructional packages geography based on ecosystem (PIGeoEk) have been developed and instrument test used for measuring knowledge about the concepts of ecosystem and relation to students’ environmental behavior. The are four t-test have been applied to verify the effectiveness of Instructional Pakages. Research results indicate that there is a significant difference between mean of gain score obtained from post and pre test at treatment group compare to control group. Therefore, the instructional packages geography based on ecosystem (PIGeoEk) could be implemented in enhancing students' knowledge about the concepts of the ecosystem in relation to students’ environmental behavior effectively.


2018 ◽  
Vol 6 (2) ◽  
pp. 319
Author(s):  
Rommiyatun Zainiyah ◽  
Erti Ikhtiarini Dewi ◽  
Wantiyah Wantiyah

One of stressor in college students is taking a final project as a graduation requirement. The body'sresponse toward stress includes a rise of heart rate, diarrhea and insomnia. One of stressmanagement is affirmation relaxation (AR) technique. This research aimed to analyze the influenceof AR technique towards stress on students who take a final project in Faculty of Nursing, UniversityOf Jember. This research used a quasi experiment with Pretest-Post test approach and controlgroup design. Sampling technique that used is purposive sampling with respondent divided into twogroups, the treatment group and control group with 15 respondents for each group. Data wasanalyzed by using Dependent t test and Independent t test with 95% CI. The result showed thatthere was a significant difference stress before and after given AR technique within average 19,2 to10,87 (p = 0,001). Mean while, there was no significant difference of stress between pretest and posttest in control group within average 18,73 to 18,4 (p = 0,841). Overall, there was a difference ofstress between treatment group and control group (p =0,02, α = 0,05). The AR techniques reducestress because it can influence the sympathetic and parasympathetic nerves to produce endorphinehormone. The conclusion of this research is there was an influence of AR technique towards stresson students who Taking a Final Project in Faculty of Nursing University of Jember.Keywords: affirmation relaxation technique, stress, final project.


2021 ◽  
Vol 9 (T4) ◽  
pp. 106-110
Author(s):  
Susanti Susanti ◽  
Difran Nobel Bistara

BACKGROUND: Chronic kidney disease (CKD) is a chronic illness with complex disease which could lead to other underlying diseases such as diabetes mellitus (DM), hypertension, and dyslipidemia. Urban population must manage their illness due to their occupation. Coaching support is an advanced method to help individuals manage their illnesses, especially chronic illness. Symptoms and complaints in early-stage renal disorders tend to be mild, making it difficult to diagnose only by clinical examination. Impaired kidney function can lead to progressive kidney damage. AIM: This study aimed was to analyze the effect of coaching support in maintaining kidney function in patients with CKD. METHODS: This research used quasi-experiment with pre-test and post-test with control group design. Respondents in this study were 40 CKD patients which were taken by consecutive sampling technique and divided into two groups, namely, control group and treatment group. Data were collected using blood urea nitrogen and creatinine values observation sheet. Coaching support was divided into four steps of therapy, identify the disturbance, identify based on experience, use a family support system, and evaluating the results. Data were analyzed using paired t-test and independent t-test with a significance of p < 0.05. RESULTS: This study found that there was a significant difference in kidney function between the control group and the treatment group (p = 0.000). Coaching support interventions were effective on kidney function in patients with CKD. The implementation of coaching support went well because respondents and families were proactive. CONCLUSION: Coaching support should be applied by nurses as daily activity management of CKD patients at early stage to inhibit the kidney function damage progression.


2020 ◽  
Vol 38 (1) ◽  
pp. 7-15
Author(s):  
Hendrastutik Apriningsih ◽  
Suradi Suradi ◽  
Yusup Subagio Sutanto

Backgrounds: Chronic obstructive pulmonary disease is the leading cause morbidity and mortality worldwide. Cigarette smoke and noxious agent causing oxidative stress activated nuclear factor-κB then increase inflammatory genes releases. Epigallocatechin-3-gallate green tea have antiiinflammatory effect which can be use as addition therapy for stable COPD. Methods: This study aimed to analyze the effect of EGCG to absolute neutrophil count (ANC), serum MMP-9, %FEV1, and CAT score stable COPD patients. Clinical trials of experimental with pre-test and post-test design was conducted on 30 patients in Dr. Moewardi Hospital Surakarta from February-April 2017. Samples were taken by consecutive sampling divided into treatment group (n=15) received standard therapy and green tea capsule 2x500mg/day during 28 days and control group received only standard therapy (n=15). Decreased inflammation measured by ANC and serum MMP-9, improvement of obstruction measured by %FEV1, and clinic improvement measured by CAT score. Results: There were no significant differences (P=0.135) decrease ANC treatment group (-662.45±1446.80 µL) compared control (413.79±2292.90 µL), decrease serum MMP-9 (P=0.413) treatment group (-324.34±333.56 ng/ml) compared control (-181.21±577.52 ng/ml), %FEV1 (P=0.236) treatment group (2.56±10.77), compared control (-4.30±19.12), and significant difference (P=0.034) CAT score treatment group (-1.07±1.16) compared control (-0.20±1.08). Conclusions: The addition of green tea capsule 2x500 mg/day during 28 days was significantly lowered CAT score, decreasing ANC, serum MMP-9 and increasing %FEV1 but not significant. (J Respir Indo 2018; 38(1): 7-15)


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