scholarly journals Phytochemical Screening of the Hydro Ethanolic Extract of Brassica oleracea Var. Italica Plant Extract

2018 ◽  
Vol 9 (07) ◽  
Author(s):  
Shah M. A. ◽  
Himanshu . ◽  
Sarker M M R ◽  
Banu Afreen
Keyword(s):  
Traditional Medicine ◽  
Brassica Oleracea ◽  
Plant Extract ◽  
Cardiac Glycosides ◽  
Ethanolic Extract ◽  
Acid Group ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Pharmaceutical Industries ◽  
Potent Drug

Objective: This study was designed elucidate the Phytochemicals of the widely-used plant Brassica oleracea var. Italica. Method: Hydroethanolic extracts of Brassica oleracea var. Italica plant extract was investigated. A small portion of the hydroethanolic extracts of Brassica oleracea var. Italica was subjected to the phytochemical test using Trease and Evans and Harbourne methods to test for the presence of alkaloids, tannins, reducing sugars, saponins, terpenoids, phenols, flavonoids and Anthraquionones. Result: The phytochemical analysis revealed the presence of alkaloids, saponins, tannins, flavonoids, terpenoids, coumarins, quinines, cardiac glycosides, Xanthoproteins, glycosides, steroids, phenols, resins, carboxylic acid group in varying concentrations. The present study provides evidence that Hydro ethanolic extracts of Brassica oleracea var. Italica contains medicinally important bioactive compounds and this justifies the use of plant species as traditional medicine for treatment of various diseases. Conclusion: Thus, from the present study the plant leaf extracts of Brassica oleracea var. Italica showed an abundant production of Phytochemicals as secondary metabolites and they can be used in the pharmaceutical industries for producing a potent drug. The studies result of the above two plants gives a basis of its use in traditional medicine to manage ailments and disorders.

scholarly journals PHYTOCHEMICAL EVALUATION OF PUNICA GRANATUM L. LEAF EXTRACT

2017 ◽  
Vol 9 (4) ◽  
pp. 14 ◽  
Author(s):  
P. Sreedevi ◽  
K. Vijayalakshmi ◽  
R. Venkateswari
Keyword(s):  
Metabolic Diseases ◽  
Ethanolic Extract ◽  
Punica Granatum ◽  
Leaf Extracts ◽  
Bioactive Substances ◽  
Pharmaceutical Ingredients ◽  
Wide Range ◽  
Leaf Powder ◽  
Potent Drug ◽  
Punica Granatum L

Objective: This study was conducted to assess the phytochemical constituents in Punica granatum L. Leaf extracts (PGLE) using standard methods.Methods: The leaf powder was extracted using solvents namely aqueous, hydroalcohol, ethanol, ethyl acetate and n-hexane. Qualitative and Quantitative phytochemical screenings of PGLE were assessed by standard methods.Results: All the leaf extracts were positive for a wide range of bio-active compounds except n-hexane. The result has showed that the maximum amount of total phenols (394.16 mg/g DW of extract), total tannins (210.5 mg/g DW of extract), flavanoids (147.4 mg/g DW of extract) and total triterpenoids (112 mg/g DW of extract) were noted in ethanolic extract of P. granatum leaf (EPGL). The biological assay revealed that relevant amount of carbohydrate, protein, lipid and alkaloid in EPGL.Conclusion: The findings of this study concluded that the EPGL had potential bioactive substances that may be used as pharmaceutical ingredients for formulation of new or prospective potent drug to cure wide range of metabolic diseases.

scholarly journals Quantitative and Qualitative Identification of Phytochemical Constituents of Sida Rhombifolia Leaves Extract

2019 ◽  
Vol 8 (2S4) ◽  
pp. 403-408
Keyword(s):  
Quantitative Analysis ◽  
Cardiac Glycosides ◽  
Total Content ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Biological Compounds ◽  
Potential Sources ◽  
Pharmaceutical Industries ◽  
Phytochemical Constituents ◽  
Sida Rhombifolia

Anti-inflammatory, analgesic, antimicrobial, anti-arthritic, antibacterial, antispasmodic, hypoglycaemic and hepatoprotective characteristics of Sida rhombifolia are included in the Malvaceae family. Most of the plant belongs to the Malvaceae family, as they comprise a number of phytochemicals and biological compounds, are potential sources of different medications. This is study on Sida rhombifolia leaf extract's tophytochemical constituen ts. Phytochemical screening results in herbal stan-dardizat ion and preparation and may relate the components to thei r medicinal / pharmacological uses. The qualitative phytochemical analysis has shown that the extract is positive for saponins, flavonoids, alkaloids, phenols and same extract is negative for carbohydrate, tannins, glycosides, cardiac glycosides, terpenoids, coumarins, steroid& phytosteroids, phlobutanins, anthraquinones. Quantitative analysis of phytochemicals includes the estimation of flavonoid, tannin and total content of phenol. The result suggest that the Sida rhombifolia leaves extract consist plenty of phytochemicals beneficial in alternative medical and pharmaceutical industries.

scholarly journals PRELIMINARY PHYTOCHEMICAL STUDIES OF SELECTED MEDICINAL PLANT PACHYGONE OVATA (POER.) HOOK.F. & THOMS FROM MENISPERMACEAE FAMILY FOR BIOACTIVE CONSTITUENTS

2018 ◽  
Vol 5 (2) ◽  
pp. 50-52
Author(s):  
Renjini Haridas ◽  
Radhakrishnan G ◽  
Reshma R ◽  
Sumathi P
Keyword(s):  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Cardiac Glycosides ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Phytochemical Studies ◽  
Leaf Part ◽  
Important Medicinal Plant

The present study deals with the phytochemical examination of Pachygone ovata (Poer.) Hook.f.& Thoms., an important medicinal plant from menispermaceae family. Leaf and Stem extracts were prepared by using different solvents systems and phytochemical screening was performed using the standard methods given by Harborne. Leaf and stem extracts were prepared from aqueous and organic solvents like petroleum ether, acetone, ethyl acetate and ethanol. Qualitative phytochemical analysis of the petroleum ether, acetone,ethyl acetate, ethanol and aqueous extracts prepared from P. ovata leaf and stem part. Leaf part revealed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, phenols and tannins. Stem part revealed the presence of alkaloids, flavonoids, glycosides, Resin, Steroids, phenols and tannins. The ethanolic extract showed higher amount of secondary metabolites than the other solvent extracts. This observation becomes important in the context of the therapeutically and drug applications of P. ovata.

Preliminary Phytochemical Analysis and its Antimicrobial and Antioxidant Activitiy of Leaf Extract of Justicia Adhatoda

2021 ◽  
Vol 7 (4) ◽  
Keyword(s):  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Antibacterial Study ◽  
Antibacterial Screening ◽  
Justicia Adhatoda

Justicia Adhatoda is one of the medicinal plant native to Asia that is widely used in antidiabetic, anti inflammatory, antibacterial properties, treatment of cough and other respiratory ailments. In the present study, few steps procedure method was used to extract the major compound in the leaves.From the leaf extracts, phytochemical tests, measurement of antioxidant activity andantibacterial activities were studied. The results showed that the ethanol leaf extracts contains Phenols, Flavonoids, Tannis, Alkaloids and Saponins. The antioxidant activitywas evaluated in-vitro by radical scavenging DPPH, FRAP and H2O2 method and the disc diffusion method was employed for the antibacterial study on Pseudomonas, Staphylococcus, Bacillus, E.coli using chloramphinicol antibiotic as standard. Antibacterial screening showed that the maximum zone of inhibition for ethanolic extract against E.Coli is around 1.35 cm.

Anticonvulsant activity of Nylandtia spinosa L. Dumont (Polygalaceae) aqueous and methanol leaf extracts in mice

2008 ◽  
Vol 27 (11) ◽  
pp. 811-818 ◽  
Author(s):  
GJ Amabeoku
Keyword(s):  
Aqueous Extract ◽  
Anticonvulsant Activity ◽  
Plant Material ◽  
Cardiac Glycosides ◽  
Reducing Sugars ◽  
Methanol Extract ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Anticonvulsant Property ◽  
Aqueous Leaf Extract

Aqueous and methanol leaf extracts of Nylandtia spinosa L. Dumont (Polygalaceae) were evaluated for anticonvulsant activity against tonic seizures produced in mice by pentylenetetrazole (PTZ), bicuculline, picrotoxin, and N-methyl-DL-aspartic acid (NMDLA). Aqueous leaf extract of N. spinosa (50–400 mg/kg, i.p.) and methanol extract (50–400 mg/kg, i.p.) significantly attenuated PTZ (95 mg/kg, i.p.)-induced tonic seizures. Doses of 400 mg/kg (i.p.) and 100–400 mg/kg (i.p.) of aqueous extract of N. spinosa significantly delayed the onset of tonic seizures elicited by bicuculline (35 mg/kg, i.p.) and picrotoxin (12 mg/kg, i.p.), respectively. Methanol extract (200–400 mg/kg, i.p.) and (50–400 mg/kg, i.p.) significantly delayed the onset of tonic seizures induced by bicuculline (35 mg/kg, i.p.) and picrotoxin (12 mg/kg, i.p.), respectively, whereas 400 mg/kg (i.p.) significantly reduced the incidence of picrotoxin (12 mg/kg, i.p.)-induced seizures. Both aqueous and methanol leaf extracts of N. spinosa did not affect NMDLA (400 mg/kg, i.p.)-induced tonic seizures. Phenobarbitone (12.5 mg/kg, i.p.) and diazepam (0.5 mg/kg, i.p.) antagonized tonic seizures induced by PTZ (95 mg/kg, i.p.), bicuculline (35 mg/kg, i.p.), and picrotoxin (12 mg/kg, i.p.) but did not affect NMDLA (400 mg/kg, i.p.)-induced seizures. Phenytoin (30 mg/kg, i.p.) did not alter the tonic seizures produced by either PTZ (95 mg/kg, i.p.), bicuculline –2-(35 mg/kg, i.p.), or picrotoxin (12 mg/kg, i.p.). The results obtained indicate that both aqueous and methanol leaf extracts of N. spinosa possess anticonvulsant property, thus justifying the use of the plant by traditional medicine practitioners in the treatment of epilepsy. The relatively high LD50 of greater than 3600 mg/kg (p.o.) and 1780 mg/kg (i.p.) obtained with the aqueous extract suggest that the plant is relatively safe in mice. The phytochemical analysis carried out showed the presence of tannins, saponins, reducing sugars, alkaloids, flavonoids, triterpene steroids, and cardiac glycosides in the plant material.

Preliminary phytochemical screening, isolation and structural elucidation of chloroform leaf extracts of Maesa lanceolata

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Cardiac Glycosides ◽  
Leaf Extract ◽  
Chloroform Extract ◽  
Phytochemical Analysis ◽  
Spectroscopic Methods ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Standard Procedures ◽  
Phytochemical Constituents ◽  
First Time

The aim of this study was to screen the phytochemical constituents, isolate and elucidate the structure of chloroform extracts from the leaves of Maesa lanceolata. The qualitative phytochemical analysis of the leaf extract of Maesa lanceolata was done following standard procedures and the tests revealed the presence of cardiac glycosides and saponins in the crude extracts. The chloroform extract of the leaves of M. lanceolata led to the isolation of one compound, C1. Structure determination was accomplished by means of spectroscopic methods (IR, 13C and 1H NMR). According to the spectral data (IR, 1HNMR, 13CNMR and DEPT), probably Myrsenene was identified which is reported for the first time from M. lanceolata.

scholarly journals Phytochemical Screening of Aqueous, Ethanolic and Methanolic Extracts of Morus mesozygia Linn. Stapf., Leaves

2020 ◽  
pp. 38-46
Author(s):  
Marcella Tari Joshua ◽  
Edna O. Nwachukwu ◽  
N. Boisa ◽  
Nsirim Nduka
Keyword(s):  
Fine Powder ◽  
Ethanolic Extract ◽  
Cross Sectional Study ◽  
Complex Compounds ◽  
Phytochemical Analysis ◽  
Distilled Water ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Cross Sectional ◽  
Methanolic Extracts

Aim: This study focused on the phytochemical screening of aqueous, ethanolic and methanolic leaf extracts on the species Morus mesozygia linn. Study Design: This study was a cross-sectional study. Place and Duration of Study: This study was carried out at the Plant Anatomy and Physiology Research Laboratory, University of Port Harcourt, between July, 2018 and November, 2018. Methodology: Morus mesozygia linn leaves were collected and washed with distilled water, air dried for seven days and milled into fine powder. Maceration method was use to extract the powdered leaf into a brownish paste using three different solvents; distilled water, ethanol and methanol. The different plant extracts were subjected to qualitative phytochemical screening for alkaloids, flavonoids, saponins, carbohydrates, tannins and anthraquinones. Quantitative phytochemical analysis was done using a Gas chromatography – Mass Spectroscopy machine. Results: Results showed that the powdered Morus mesozygia linn leaves contained alkaloids, flavonoids, saponins, carbohydrates, tannins, but not anthraquinones. The methanolic and aqueous leaf extracts contained high amounts of alkaloids, flavonoids, saponins, carbohydrates and tannins, while the ethanolic extract also contained high amounts of the aforementioned phytochemicals in the same proportion, but had saponins in moderate amounts. The result of the GC-MS analysis showed that the three extracts contained complex compounds in varying amounts. Conclusion: Phytochemical screening test of Morus mesozygia has revealed the presence of the substances like alkaloids, saponins, flavonoids, oils, phenolic compounds, tannins and some complex compounds discovered using GC-MS technique.

scholarly journals EFFECT OF VAM FUNGI ON PHYTOCHEMICAL AND ANTIBACTERIAL ACTIVITY IN SOLANUM SURATTENSE L.

2016 ◽  
Vol 3 (1) ◽  
pp. 170-173
Author(s):  
S Pria ◽  
O.N. Shanmugapriya
Keyword(s):  
Antibacterial Activity ◽  
Cardiac Glycosides ◽  
Ethanolic Extract ◽  
Fungal Species ◽  
Phytochemical Analysis ◽  
Onion Plant ◽  
Vam Fungi ◽  
Solanum Surattense ◽  
Treated Plant ◽  
Pot Culture

In this study six fungal species were isolated from soil samples. Among the six species Glomus fasiculatum and Gigaspora marigarita noted in dominant level. So both are used as bioinoculant and were mass multiplied in onion plant by pot culture. After pot culture, biochemical and antibacterial activity were analysed from the treated plant Solanum surattense. In the phytochemical analysis falvonoids, tannins , terpenoids, cardiac glycosides and saponins were noted. Antibacterial activity of ethanolic extract of Solanum surattense  against clinical isolates were analyzed and show the more activity noted in K.pneumoniae when compared with Bacillus subtilis, Streptococcus pyogens, E.coli respectively.

In-vitro Antioxidant and Antimicrobial activities of Murraya koiengii and Ficus palmata leaf extracts from North-western Himalayan Region

2021 ◽  
pp. 4332-4336
Author(s):  
Avinash Sharma ◽  
Rupak Nagraik ◽  
Somesh Sharma ◽  
P. K. Chauhan
Keyword(s):  
Plant Extract ◽  
Antimicrobial Activities ◽  
Himalayan Region ◽  
Dependent Manner ◽  
Leaf Extracts ◽  
Western Himalayan Region ◽  
Pharmaceutical Industries ◽  
Ficus Palmata ◽  
North Western ◽  
Antioxidant And Antimicrobial Activities

Plant based compounds are used as an alternate to chemically synthesized compounds in pharmaceutical industries. The main aim of the study was to evaluate the phytochemical, antioxidant and antimicrobial activities of leaf extract of two medicinal plants i.e. Murraya koiengii and Ficus palmata collected from north-western Himalayan region. The phytochemical screening of these plant extract showed the presence of alkaloids, phenols, tannins, flavonoids and the absence of terpenoids and saponins. The qualitative data analysis confirmed the highest level of phytochemicals in Murraya koiengii plant extract as compared to Ficus palmata. The antioxidant results show the dose dependent manner. The minimum inhibitory concentration (MIC) value of the extracts was found at concentration of 1.5625 mg/ml for Murraya koiengii and 3.125 mg/ml for Ficus palmata. The present study supports the use plant extracts as an alternative medicine in the ailment of many diseases.

scholarly journals Antibacterial effect of Ocimum gratissimum against bacteria from paediatric diarrhoeal stool samples in the tropics

2017 ◽  
Vol 36 (3) ◽  
pp. 64-74
Author(s):  
Odebisi-Omokanye M. B. ◽  
T. O. Agbabiaka ◽  
R. F. Zakariyah ◽  
O. Sanya
Keyword(s):  
Antimicrobial Agents ◽  
Low Cost ◽  
Ethanolic Extract ◽  
Selective Inhibition ◽  
Resistant Bacteria ◽  
Phytochemical Analysis ◽  
Aqueous Extracts ◽  
Leaf Extracts ◽  
Ocimum Gratissimum ◽  
Stool Samples

The upsurge in the prevalence of side effects of many synthetic antimicrobial agents and incidence of multidrug resistant bacteria has spurred scientists on the research for plant based antimicrobial of therapeutic potentials. Ocimum gratissimum presents such potential of high medicinal value. This plant is used in Nigeria traditionally as condiments and for the treatment of various ailments such as pyorrhea, dysentery and bronchitis. Aqueous and ethanolic leaf extracts of O. gratissimum was screened for antibacterial activity against bacteria recovered from paediatric diarrhoea stool samples. Both extracts were found to exhibit selective inhibition against the isolates. The diameter zones of inhibition exhibited by the extracts were between 2.50±0.50mm – 26.00±1.00mm. The ethanolic and aqueous extracts had minimum inhibitory concentrations that ranged between 15 and 25mg/ml, the extracts were bacteriostatic. Ethanolic extract showed more inhibitory effects compared to the aqueous extracts. The phytochemical analysis of both extracts revealed the presence of saponins, alkaloids, flavonoids, phenolics, tannins and glycosides, volatile oils, proteins and amino acids and terpernoids. In view of the efficacy of these extracts in inhibiting the growth of diarrhoea- causing organisms in paediatric stool samples, the utilization of the extracts in the formulation of new antibacterial drugs for the treatment of gastroenteritis caused by these organisms is strongly recommended especially when the availability and low cost of this medicinal plant are put into strong consideration.Keywords: Ocimum, diarrhoea, paediatric, stool, antibacterial

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