Evaluation of Invitro Antioxidant Activity of Flowers of Tagetes erecta

Tagetes erecta (T. erecta) is an herbaceous species, having wide ethno medicinal and traditional uses. It demonstrates different pharmacological activities like antioxidant, antimicrobial and anti depressant. The present study deals with the estimation of antioxidant properties of four solvent extracts of flowers of T. erecta. The study aims to discover potent extracts as a novel source of natural antioxidant. The crude extracts; Petroleum ether (PE), Chloroform (CF), Ethyl Acetate (EA) and Methanol (MET) were screened for their free radical scavenging capacity and reducing powers using DPPH, Superoxide and Reducing power assay. Ascorbic acid was used as standard. All the extracts show considerable antioxidant potential. However, methanolic extract can strongly scavenge DPPH and superoxide radical with significant IC50 value range 30.08 ± 0.98 µg/mL and 64.22±0.04 µg/mL respectively. The IC50 value of the extracts follows the order; MET less than EA less than PE less than CF. Lowest IC50 value indicates highest antioxidant potential. The reducing power of extracts were in the order; CF less than PE less than EA less than Met

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Evaluation of the Antioxidant Capacities and Cytotoxic Effects of Ten Parmeliaceae Lichen Species

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/3169751 ◽

2016 ◽

Vol 2016 ◽

pp. 1-11 ◽

Cited By ~ 7

Author(s):

C. Fernández-Moriano ◽

E. González-Burgos ◽

P. K. Divakar ◽

A. Crespo ◽

M. P. Gómez-Serranillos

Keyword(s):

Methanol Extract ◽

Phenolic Content ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Cytotoxic Activities ◽

Pharmacological Activities ◽

Antioxidant Power ◽

Scavenging Capacity ◽

Dpph Scavenging

Parmeliaceae represents the largest and widespread family of lichens and includes species that attract much interest regarding pharmacological activities, due to their production of unique secondary metabolites. The current work aimed to investigate the in vitro antioxidant and cytotoxic activities of the methanol extracts of ten Parmeliaceae species, collected in different continents. Methanol extraction afforded high phenolic content in the extracts. The antioxidant activity displayed by lichens was evaluated through chemical assays, such as the ORAC (Oxygen Radical Absorbance Capacity) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities and the ferric reducing antioxidant power (FRAP). A moderately positive correlation was found between the phenolic content and the antioxidant properties for all the species: R: 0.7430 versus ORAC values, R: 0.7457 versus DPPH scavenging capacity, and R: 0.7056 versus FRAP reducing power. The methanol extract of Flavoparmelia euplecta exhibited the highest ORAC value, the extract of Myelochroa irrugans showed the maximum DPPH scavenging capacity, and Hypotrachyna cirrhata methanol extract demonstrated the highest reducing power. Further, the cytotoxic activity of the ten species was investigated on the human cancer cell lines HepG2 and MCF-7; Myelochroa irrugans exhibited the highest anticancer potential. The pharmacological activities shown here could be attributed to their phytochemical constituents.

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Physiochemical properties and antioxidant potential of Persea Americana seed oil

10.31221/osf.io/jtf4s ◽

2019 ◽

Author(s):

Chem Int

Keyword(s):

Seed Oil ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Acid Value ◽

Persea Americana ◽

Antioxidant Potential ◽

Physiochemical Properties ◽

Physiochemical Parameters ◽

Ic50 Value

Oil extracted from Persea Americana seed was assayed for its physiochemical properties and antioxidant potential using various standard methods. The oil content of the seed was found to be < 10%. Brownish-red color oil was liquid at room temperature, with specific gravity of 0.91±0.02 g/mL. Other physiochemical parameters determined were; acid value (4.51±0.08 mgKOH/g), %FFA (2.26±0.08), peroxide value (2.40±0.57 mgO2/Kg), ester value (31.26±0.03 mgKOH/g), saponification value (35.76±0.07 mgKOH/g) and iodine value (23.5±0.07). The results of the antioxidant activities of the seed oil showed that the flavonoid content (80.00±1.41 mgQE/g) was ~10 folds higher than the phenolic content (8.27±0.06 mgGAE/g). The DPPH radical scavenging value was found to be 51.54±0.25% with an IC50 value of 4.68±0.02 mg/mL and reducing power with an average absorbance of 0.85±0.01 and an IC50 value of 0.001±0.02 mg/mL. Gallic acid showed better antioxidant activities than the oil studied. The results obtained in this study showed that Persea Americana seed oil has nutritional, industrial as well as medicinal potentials.

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Phenolic compounds and antioxidant properties of wheat fermented with Agaricus brasiliensis and Agaricus bisporus

FEMS Microbiology Letters ◽

10.1093/femsle/fnaa213 ◽

2020 ◽

Author(s):

Fei-Hong Zhai ◽

Yan-Fei Chen ◽

Yong Zhang ◽

Wen-Jing Zhao ◽

Jian-Rong Han

Keyword(s):

Phenolic Compounds ◽

Agaricus Bisporus ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Ultra Performance Liquid Chromatography ◽

Fermentation Products ◽

Agaricus Brasiliensis ◽

Scavenging Capacity ◽

Dpph Radical Scavenging

Abstract Solid-state fermentation with Agaricus brasiliensis and Agaricus bisporus on whole grain wheat was carried out. Phenolic compounds and antioxidant properties of fermented wheat were determined. The results showed that the maximum values of polyphenols contents in wheat fermented with A. brasiliensis and A. bisporus reached respectively (3.16 ± 0.21) and (3.93 ± 0.23) mg GAE/g, which were 2.90 and 3.61 times of unfermented control. By employing ultra performance liquid chromatography coupled to mass spectrometry (UPLC-MS), 18 kinds of phenolic compounds were identified from fermented wheat. Compared with control, only 4-hydroxy-benzaldehyde was the same compound. It indicated that fermentation with the two fungi changed polyphenols contents and phenolic compounds composition in wheat to a great extent. Among these phenolic compounds, except for 4-hydroxy-benzaldehyde, 4-hydroxy-benzoic acid and β-N-(γ-glutamyl)-4-formylphenylhydrazine, other 15 kinds of phenolic compounds were first identified from mushroom samples (including fruit bodies, mycelia and fermentation products). DPPH radical scavenging capacity, reducing power, ferrous ion chelating ability and inhibition of lipid peroxidation of fermented wheat were significantly stronger than control (P < 0.05).

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Determination of in vitro antioxidant activity of the leaves extracts of Ehretia pubescens

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i4.3308 ◽

2020 ◽

Vol 11 (4) ◽

pp. 6262-6267

Author(s):

Krishnamoorthy Meenakumari ◽

Giridharan Bupesh ◽

Mayur Mausoom Phukan

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Biological Properties ◽

Scavenging Activity ◽

Reducing Power Assay

The foods from plants were known to ensure against degenerative diseases and maturing because of their antioxidant activitycredited to their high content. Information on antioxidant activity of Indian medicinal plant is abundant. To the best of our knowledge, biological properties have not been accounted in the literature for this species of . As a point, this is the first results to assess the anti-oxidant activity of the plant which belongs to the family . The antioxidant activity of Methanol, , Ethyl acetate and Aqueous extracts of E. was determined using the DPPH free radical scavenging activity, ABTS radical scavenging activity and reducing power assay. The DPPH scavenging activity showed higher activity observed in extract (63%) of E. than (54%), (44%) and aqueous (30%). the ABTS assay inhibition in extract (58%) than (43%), (38%) and aqueous (32%) extracts. The reducing power assay of different extracts was increased in extract (54%) than (40%), (34%) and aqueous (28%) extracts. Overall, the and ethyl acetate extract had higher antioxidant properties than other extract. However, in this study, extracts exhibit great potential for antioxidant activity and may be useful for their nutritional and medicinal functions.

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Antioxidant Potential, Antinutrients, Mineral Composition and FTIR Spectra of Legumes Fermented with Rhizopus oligosporus

Food Technology and Biotechnology ◽

10.17113/ftb.59.04.21.7319 ◽

2021 ◽

Vol 59 (4) ◽

Author(s):

Barinderjeet Singh Toor ◽

Amarjeet Kaur ◽

Param Pal Sahota ◽

Jaspreet Kaur

Keyword(s):

Mineral Composition ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Pigeon Pea ◽

Ftir Spectra ◽

Antioxidant Potential ◽

Total Phenolic ◽

Rhizopus Oligosporus ◽

Antioxidant Power

Research background. Legumes are superior sources of macro- and micronutrients which can be further enhanced by fermentation. This can assist in addressing the food security concerns. The present study aims to determine the effect of fermentation by Rhizopus oligosporus on nutritional and antinutritional composition of some commonly consumed legumes. Experimental approach. Chickpea (kabuli and desi), pigeon pea, and soybean were fermented with Rhizopus oligosporus (at 34 °C for 52 h), dried at 45 ºC for 16-18 h and milled. Unfermented and fermented flours were evaluated for antioxidant potential, phenolic composition, antinutrients, mineral composition and FTIR spectra. Results and conclusions. Fermentation significantly (p<0.05) enhanced the total phenolic and flavonoid contents, and antioxidant properties (radical scavenging activity, reducing power, ferric reducing antioxidant power and metal chelation) of chickpea kabuli and desi, and soybean. Although fermented pigeon pea exhibited excellent antioxidant properties, the effect of fermentation on such properties was either minimal or insignificant. Additionally, specific phenolics were quantified using HPLC which showed higher concentration of certain compounds such as chlorogenic acid, p-hydroxybenzoic acid, gallic acid and vanillic acid in fermented legumes. Phytic acid in all the fermented legumes reduced (p<0.05), however trypsin inhibition increased (p<0.05). In chickpea kabuli and desi, and pigeon pea, saponins increased (p<0.05) while they decreased in soybean. Tannins enhanced (p<0.05) in chickpea desi, pigeon pea and soybean and reduced (p<0.05) in chickpea kabuli. Furthermore, fermentation enhanced the content as well as estimated bioavailability of minerals. FTIR spectrum of unfermented and fermented legumes showed the presence of several functional groups and modifications in the molecular structure after fermentation. Novelty and scientific contribution. To our knowledge, this is the first study wherein legume (chickpea kabuli and desi, pigeon pea and soybean) fermentation by Rhizopus oligosporus has been assessed for nutritional and antinutritional profile, and FTIR spectra, which concluded that the treatment resulted in an optimal balance of nutrients and antinutrients. The process was established as a potential tool and thus can be proposed in the development of legume based novel functional foods which might help in tackling the concerns of nutritional security.

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COMPARATIVE EVALUATION OF ANTIDIABETIC AND ANTIOXIDANT POTENCY OF DIFFERENT EXTRACTS OBTAINED FROM MEMECYLON SPECIES

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.15695 ◽

2017 ◽

Vol 9 (2) ◽

pp. 187

Author(s):

Tumkur Ramasetty Bharathi ◽

Harishchandra Sripathy Prakash

Keyword(s):

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Methanol Extracts ◽

Soxhlet Apparatus ◽

Antioxidant Potency ◽

Reducing Power Assay ◽

Different Doses

Objective: Memecylon species is being extensively used in traditional medicine for the treatment of skin disorders and it is proved to possess antidiabetic and anti-inflammatory properties. The present investigation was to study the effect of different solvent extracts of five Memecylon species such as M. umbellatum, M. talbotianum, M. edule, M. malabaricum and M. wightii on antidiabetic and antioxidant effects.Methods: Plant extracts were prepared using soxhlet apparatus using different solvents such as hexane, ethyl acetate, methanol and water and obtained extracts were subjected to antidiabetic (α-amylase and α-glucosidase inhibition assays) and antioxidant (2, 2-Diphenyl-2-Picryl Hydrazyl hydrate (DPPH), 2,2-Azino-bis (3-ethyl benzothiazoline-6-Sulfonic acid)diammonium salt (ABTS), Superoxide radical scavenging assay (SRSA) and reducing power assays) evaluated at different doses.Results: Methanol extracts of all five Memecylon species exhibited effective antidiabetic and antioxidant properties among them methanol extracts of M. malabaricum and M. talbotianum have highest biological activity. For α-amylase IC50 value for both M. malabaricum and M. talbotianum was found to be 100 and 130 µg/reaction and IC50 value for α-glucosidase was found to be 6.1 and 7.8 µg/reaction respectively. For DPPH the IC50value was found to be 190 µg/reaction, for ABTS 31-39 µg/reaction, for SRSA 950-1200 µg/reaction and for reducing power assay 420-490 µg/reaction respectively.Conclusion: The results indicate that methanol extracts of M. malabaricum and M. talbotianum possess potent in vitro antidiabetic and antioxidant activities compared to other Memecylon species.

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Metabolic Profiling of Nine Mentha Species and Prediction of Their Antioxidant Properties Using Chemometrics

Molecules ◽

10.3390/molecules24020258 ◽

2019 ◽

Vol 24 (2) ◽

pp. 258 ◽

Cited By ~ 3

Author(s):

Yun Park ◽

Seung-A Baek ◽

Yongsoo Choi ◽

Jae Kim ◽

Sang Park

Keyword(s):

Phenolic Compounds ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Lipophilic Compounds ◽

Antioxidant Capacities ◽

Metabolite Profiles ◽

Pls Analysis ◽

Reducing Power Assay ◽

Β Carotene

Mentha species are well recognized for their medicinal and aromatic properties. The comprehensive metabolite profiles of nine Mentha species have been determined. The extracts of these Mentha species were also screened for antioxidant and free radical scavenging activities. Forty-seven hydrophilic and seventeen lipophilic compounds were identified and quantified from the selected Mentha species. Also, eleven phenolic compounds, riboflavin and eight carotenoids were present, and their composition and content varied among the various Mentha species. The different Mentha species exhibited a range of antioxidant potencies. Horse mint especially exhibited the strongest antioxidant capacities (1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydrogen peroxide, and reducing power assay) among the nine Mentha species. A difference between different samples from the same species was not observed by multivariate analysis. A high correlation between metabolites involved in closely linked biosynthetic pathways has been indicated. The projection to latent structure method, using the partial least squares (PLS) method, was applied to predict antioxidant capacities based on the metabolite profiles of Mentha leaves. According to the PLS analysis, several carotenoid contents, such as E-β-carotene, 9Z-β-carotene, 13Z-β-carotene and lutein, as well as phenolic compounds, showed a positive relationship in reducing the power of Mentha extracts. Horse mint is a good candidate because of its high antioxidant efficacy among the nine Mentha species included in the study.

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Foliar anti-diabetic and antioxidant potential of a promising accession of Amaranthus hypochondriacus L.: GC-MS based evidences

The Journal of Phytopharmacology ◽

10.31254/phyto.2018.7204 ◽

2018 ◽

Vol 7 (2) ◽

pp. 121-126

Author(s):

Manashi Aditya ◽

◽

Soumen Bhattacharjee ◽

Keyword(s):

Lipid Peroxidation ◽

Hydroxyl Radical ◽

Leaf Extract ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Antioxidant Potential ◽

Amaranthus Hypochondriacus ◽

Chelating Activity ◽

Metal Chelating

The present study makes an effort to investigate the foliar neutraceutical potential of a promising green accession of a seed amaranth (Amaranthus hypochondriacus, accession no. IC94661) based on anti-lipid peroxidation property, reducing power, metal chelating activity, hydroxyl radical scavenging property, antidiabetic factor and GC-MS based identification of hydroxyl containing phytochemicals. Methanol and aqueous fractions possessed exhibited better anti-lipid peroxidation, reducing, hydroxyl radical scavenging, and metal chelating properties in the experimental accession. Estimation of anti-diabetic factors from the young leaf extract also revealed significantly high α-glucosidase and α- amylase inhibition properties. When GC-MS study was carried out, it exhibited presence of several hydroxyls containing phytochemicals in the accession, some of which are having antioxidant properties. Taken as a whole, the data not only provide evidence of rich sources of marker antioxidant properties but also the availability of several phytochemicals with hydroxyls, in support of its rich pharmaceutical potential

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Chemical Composition and Antioxidant Properties of Essential Oils from Peppermint, Native Spearmint and Scotch Spearmint

Molecules ◽

10.3390/molecules24152825 ◽

2019 ◽

Vol 24 (15) ◽

pp. 2825 ◽

Cited By ~ 9

Author(s):

Zhaohai Wu ◽

Bie Tan ◽

Yanhong Liu ◽

James Dunn ◽

Patricia Martorell Guerola ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oils ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

C Elegans ◽

Reducing Power Assay

Natural antioxidants have drawn growing interest for use in animal feed and the food industry. In the current study, essential oils (EOs) obtained from hydrodistillation of three mentha species, including Mentha piperita (peppermint), Mentha spicata (native spearmint) and Mentha gracilis (Scotch spearmint), harvested in the Midwest region in the United States, were analyzed for their chemical composition using gas chromatography-mass spectrometry, and their antioxidant properties were assessed through chemical assays, in vitro cell culture modeling and in Caenorhabditis elegans (C. elegans). The activity of ferric iron reduction and free-radical scavenging capacity were assessed through chemical-based assays, including the reducing power assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, and Trolox equivalent antioxidant capacity assay (TEAC). Subsequently, the capacity of EOs to mitigate lipid peroxidation was analyzed at various doses using fresh liver homogenates from pigs. A porcine jejunum epithelial cell line (IPEC-J2) was employed as in vitro model to study the cellular antioxidant activity of the mint EOs. Finally, the effectiveness of mint EOs to alleviate acute systemic oxidative damage were evaluated in vivo using C. elegans. Data were analyzed by the MIXED procedure of SAS. Contrast statement was performed to assess linear or quadratic effects of mint EOs given at various doses. All three EOs are mostly composed of monoterpenes and their derivatives (76–90%), but differed in the major compounds, which are menthol and menthone (50%) in peppermint EO and carvone (70%) in spearmint EOs. Three mint EOs demonstrated prominent radical scavenging and Fe3+ reducing activity in chemical-based assays. In comparison with native and Scotch spearmint EOs, peppermint EO had the lowest (p < 0.05) half maximal effective concentration (EC50) in DPPH and TEAC assays and higher efficacy in the reducing power assay. All three EOs exhibited equivalent activity in mitigation of chemical-induced lipid peroxidation in liver tissues in a dose-dependent manner (linear, p < 0.001). The maximal cellular antioxidant activity (CAA) was observed at 5 µg/mL for peppermint, and 100 µg/mL for native and Scotch spearmint EOs. The addition of 25 µg/mL of both spearmint EOs increased (p < 0.05) cellular concentrations of glutathione in H2O2-treated IPEC-J2 cells, suggesting enhanced endogenous antioxidant defense. Supplementation of 100 µg/mL of peppermint or Scotch spearmint EO significantly increased (p < 0.05) the survival rate of C. elegans in response to H2O2-induced oxidative stress. The protective effect is comparable to that of supplementation of 10 µg/mL of ascorbic acid. However native spearmint EO failed to reduce the death rate within the same supplementation dose (10–200 μg/mL).

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In vitro Evaluation of Antioxidant Potential of Leaves of Opuntia dillenii Haw.

Stamford Journal of Pharmaceutical Sciences ◽

10.3329/sjps.v2i1.5811 ◽

1970 ◽

Vol 2 (1) ◽

pp. 22-26 ◽

Cited By ~ 1

Author(s):

Raushanara Akter ◽

SM Raquibul Hasan ◽

Md Mokarram Hossain ◽

Taksim Ahmed ◽

Muntasir M Majumder ◽

...

Keyword(s):

Nitric Oxide ◽

Methanol Extract ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Antioxidant Potential ◽

Dependent Manner ◽

Ic50 Value ◽

Nitric Oxide Radical ◽

Opuntia Dillenii

Antioxidant potential of the 80% methanol extract of the leaves of Opuntia dillenii was evaluated using 1, 1-diphenyl, 2-picryl hydrazyl (DPPH) and nitric oxide radical scavenging, reducing power, total phenol and total flavonoid content determination assays. Preliminary phytochemical screening of the extract was also carried out, which revealed that the extract possesses flavonoids, steroids, alkaloids and tannins. The extract showed significant antioxidant activities in all antioxidant assays compared to the reference antioxidant in a dose dependent manner. In DPPH radical scavenging activity, the IC50 value of the crude extract was found to be 15.71μg/mL while the IC50 value for the reference ascorbic acid was 10.84 μg/mL. Again, the extract showed remarkable nitric oxide scavenging potential and good reducing power. Moreover, the methanol extract was found to contain high amount of phenols and flavonoids, expressed as gallic acid and rutin equivalents respectively. Based on the findings of the present study, we conclude that the methanol extract of the leaves of O. dillenii possesses remarkable antioxidant potential which may be attributed to the high amount of phenols and flavonoids present in the extract. Key Words: Opuntia dillenii; Antioxidant; Reactive oxygen species; DPPH; Nitric oxide radical DOI: 10.3329/sjps.v2i1.5811Stamford Journal of Pharmaceutical Sciences Vol.2(1) 2009: 22-26

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