Characterization of isolated lignin from eucalyptus wood chip obtained by liquid hot water process

In this study, we propose a novel optimization for lignin isolation from the eucalyptus wood chip (EWC) under hydrothermal liquid hot water (LWH) process in the presence of alkaline catalyst (NaOH). The optimal conditions were obtained employing response surface methodology (RSM). The optimal condition was 160°C for 30 min in the presence of alkaline catalyst of 0.020 M. This condition demonstrated the highest lignin extraction with solid fraction of 77.3% and 76.1%. In addition, the physicochemical structure of isolated lignin was characterized with diverse techniques. The lignin recovery from liquid phase’s GPC analysis illustrated a high average Mw/Mn (17500/7450 g/mol) while polydispersity index (2.34) was lower in comparison with the commercial organosolv lignin. The TGA analysis showed the maximum decomposition of lignin recovery at 140 to 350oC. Furthermore, the Py/GCMS analysis showed a predominance of 57.63% of syringyl units (S) over 37.37% of guaiacyl units (G) under optimal conditions. The results revealed that the integrated process was a potential approach to add more value in the employment of the agricultural waste material.

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Hydrolysis of sweet sorghum bagasse and eucalyptus wood chips with liquid hot water

Bioresource Technology ◽

10.1016/j.biortech.2012.04.031 ◽

2012 ◽

Vol 116 ◽

pp. 220-225 ◽

Cited By ~ 34

Author(s):

Qiang Yu ◽

Xinshu Zhuang ◽

Qiong Wang ◽

Wei Qi ◽

Xuesong Tan ◽

...

Keyword(s):

Sweet Sorghum ◽

Hot Water ◽

Wood Chips ◽

Sweet Sorghum Bagasse ◽

Liquid Hot Water ◽

Eucalyptus Wood ◽

Sorghum Bagasse ◽

Hydrolysis Of

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Influence of alkaline catalyst addition on compressed liquid hot water pretreatment of rice straw

Chemical Engineering Journal ◽

10.1016/j.cej.2014.12.032 ◽

2015 ◽

Vol 278 ◽

pp. 85-91 ◽

Cited By ~ 21

Author(s):

Saksit Imman ◽

Jantima Arnthong ◽

Vorakan Burapatana ◽

Verawat Champreda ◽

Navadol Laosiripojana

Keyword(s):

Rice Straw ◽

Hot Water ◽

Alkaline Catalyst ◽

Liquid Hot Water Pretreatment ◽

Liquid Hot Water ◽

Water Pretreatment ◽

Compressed Liquid ◽

Hot Water Pretreatment

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Enzymatic Hydrolysis and Fermentation of Banana Pseudostem Hydrolysate to Produce Bioethanol

International Journal of Microbiology ◽

10.1155/2021/5543104 ◽

2021 ◽

Vol 2021 ◽

pp. 1-14

Author(s):

Lesetja Moraba Legodi ◽

Daniel Coenrad LaGrange ◽

Elbert Lukas Jansen van Rensburg ◽

Ignatious Ncube

Keyword(s):

Saccharomyces Cerevisiae ◽

Enzymatic Hydrolysis ◽

Agricultural Waste ◽

Hot Water ◽

Fermentable Sugars ◽

Protein G ◽

Bioethanol Production ◽

Liquid Hot Water ◽

Separate Hydrolysis And Fermentation ◽

Holocellulose Content

Banana pseudostem (BPS) is an agricultural waste with a high holocellulose content, which, upon hydrolysis, releases fermentable sugars that can be used for bioethanol production. Different pretreatment methods, namely, 3% (w/v) NaOH, 5% (v/v) H2SO4, and liquid hot water, applied on the BPS resulted in the availability of 52%, 48%, and 25% cellulose after treatment, respectively. Saccharification of the pretreated BPS with 10 FPU/g dry solids (29.3 mg protein/g d.s) crude enzyme from Trichoderma harzianum LMLBP07 13-5 at 50°C and a substrate loading of 10 to 15% released 3.8 to 21.8 g/L and from T. longibrachiatum LMLSAUL 14-1 released 5.4 to 43.5 g/L glucose to the biomass. Ethanol was produced through separate hydrolysis and fermentation (SHF) of alkaline pretreated BPS hydrolysate using Saccharomyces cerevisiae UL01 at 30°C and 100 rpm. Highest ethanol produced was 17.6 g/L. Banana pseudostem was shown as a potentially cheap substrate for bioethanol production.

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Refining Energy Reduction and Pulp Characteristic Modification of Alkaline Peroxide Mechanical Pulp (APMP) Through Enzyme Application

TAPPI Journal ◽

10.32964/tj8.5.19 ◽

2009 ◽

Vol 8 (5) ◽

pp. 19-25 ◽

Cited By ~ 2

Author(s):

PETER W. HART ◽

DARRELL M. WAITE ◽

LUC THIBAULT, ◽

JOHN TOMASHEK ◽

MARIE-EVE ROUSSEAU ◽

...

Keyword(s):

Wood Chip ◽

The United States ◽

Enzyme Treatment ◽

Chemical Application ◽

Alkaline Peroxide ◽

Eucalyptus Wood ◽

Pulp Properties ◽

Pulp Processing ◽

Two Stages ◽

Enzyme Application

Eucalyptus wood chips were subjected to impregnation with various blends of novel fiber modify-ing enzymes before chemical pretreatment and two stages of refining using the preconditioning refiner chemical–alkaline peroxide mechanical pulping (PRC-APMP) process. Wood chip impregnation and pulp processing was con-ducted at a pilot plant in the United States. When compared under constant chemical application and at a constant 350 mL CSF, enzyme treatment reduced specific refining energy by at least 24%. The effect of one versus two stages of impregnation and of enzyme action upon several physical pulp properties was determined.

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An extensive parameter study of hydrotropic extraction of steam-pretreated birch

Biomass Conversion and Biorefinery ◽

10.1007/s13399-021-01425-w ◽

2021 ◽

Author(s):

Johanna Olsson ◽

Michael Persson ◽

Mats Galbe ◽

Ola Wallberg ◽

Ann-Sofi Jönsson

Keyword(s):

Residence Time ◽

Steam Explosion ◽

Room Temperature ◽

Initial Step ◽

Parameter Study ◽

Hydrothermal Degradation ◽

Steam Explosion Pretreatment ◽

Lignin Extraction ◽

Extraction Step ◽

Lignin Recovery

AbstractEfficient fractionation of lignocellulosic biomass is an important step toward the replacement of fossil-based products. However, the utilisation of all of the components in biomass requires various fractionation techniques. One promising process configuration is to apply steam explosion for the recovery of hemicelluloses and a subsequent hydrotropic extraction step for the delignification of the remaining solids. In this work, the influence of residence time, temperature and biomass loading on lignin recovery from birch using sodium xylene sulphonate as a hydrotrope was investigated. Our results show that residence time, temperature and biomass loading correlate positively with lignin extraction, but the effects of these parameters were limited. Furthermore, when steam explosion was implemented as the initial step, hydrotropic extraction could be performed even at room temperature, yielding a lignin extraction of 50%. Also, hydrothermal degradation of the material was necessary for efficient delignification with sodium xylene sulphonate, regardless of whether it occurs during steam explosion pretreatment or is achieved at high temperatures during the hydrotropic extraction.

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Optimization of Lignin Recovery from the Pre-Hydrolysate of Kraft-Based Dissolving Pulp Production Processes

Applied Sciences ◽

10.3390/app11010454 ◽

2021 ◽

Vol 11 (1) ◽

pp. 454

Author(s):

Adil Mazar ◽

Naceur Jemaa ◽

Waleed Wafa Al Dajani ◽

Mariya Marinova ◽

Michel Perrier

Keyword(s):

Molecular Weight ◽

Lignin Content ◽

Sulfuric Acid Concentration ◽

Dissolving Pulp ◽

Potential Candidate ◽

Lignin Extraction ◽

Dissolved Organic Matters ◽

Lignin Recovery ◽

Total Lignin ◽

Total Lignin Content

A pre-hydrolysate is an aqueous stream obtained during the production of hardwood kraft dissolving pulp. It is rich in sugars and contains dissolved organic matters. The purpose of this study is to investigate the optimization of lignin recovery from wood pre-hydrolysates and to characterize the extracted lignin. The optimal conditions for lignin extraction have been determined to be (a) a filtration temperature of 40 °C, (b) a sulfuric acid concentration of 8.5 kg·m−3, and (c) a coagulation time of 180 min. Using these conditions, high filtration rates have been obtained and the extracted lignin has a low content of impurities (8.3%), a low molecular weight (1270 Da), and a very low polydispersity (Mw/Mn = 1.22). Compared to kraft lignin, the pre-hydrolysate lignin has a much lower molecular weight and could be a potential candidate for niche applications. A high lignin recovery rate is possible (52% of the total lignin content in the pre-hydrolysate).

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Diverse Banana Pseudostems and Rachis Are Distinctive for Edible Carbohydrates and Lignocellulose Saccharification towards High Bioethanol Production under Chemical and Liquid Hot Water Pretreatments

Molecules ◽

10.3390/molecules26133870 ◽

2021 ◽

Vol 26 (13) ◽

pp. 3870

Author(s):

Jingyang Li ◽

Fei Liu ◽

Hua Yu ◽

Yuqi Li ◽

Shiguang Zhou ◽

...

Keyword(s):

Soluble Sugars ◽

Enzymatic Saccharification ◽

Hot Water ◽

Biofuel Production ◽

Bioenergy Crops ◽

Bioethanol Production ◽

Factors Affecting ◽

Liquid Hot Water ◽

Biomass Processing ◽

Banana Crops

Banana is a major fruit crop throughout the world with abundant lignocellulose in the pseudostem and rachis residues for biofuel production. In this study, we collected a total of 11 pseudostems and rachis samples that were originally derived from different genetic types and ecological locations of banana crops and then examined largely varied edible carbohydrates (soluble sugars, starch) and lignocellulose compositions. By performing chemical (H2SO4, NaOH) and liquid hot water (LHW) pretreatments, we also found a remarkable variation in biomass enzymatic saccharification and bioethanol production among all banana samples examined. Consequently, this study identified a desirable banana (Refen1, subgroup Pisang Awak) crop containing large amounts of edible carbohydrates and completely digestible lignocellulose, which could be combined to achieve the highest bioethanol yields of 31–38% (% dry matter), compared with previously reported ones in other bioenergy crops. Chemical analysis further indicated that the cellulose CrI and lignin G-monomer should be two major recalcitrant factors affecting biomass enzymatic saccharification in banana pseudostems and rachis. Therefore, this study not only examined rich edible carbohydrates for food in the banana pseudostems but also detected digestible lignocellulose for bioethanol production in rachis tissue, providing a strategy applicable for genetic breeding and biomass processing in banana crops.

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Liquid Hot Water Pretreatment and Enzymatic Hydrolysis as a Valorization Route of Italian Green Pepper Waste to Delivery Free Sugars

Foods ◽

10.3390/foods9111640 ◽

2020 ◽

Vol 9 (11) ◽

pp. 1640

Author(s):

M.A. Martín-Lara ◽

L. Chica-Redecillas ◽

A. Pérez ◽

G. Blázquez ◽

G. Garcia-Garcia ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Liquid Fraction ◽

Hot Water ◽

Effect Of Temperature ◽

Green Pepper ◽

Free Sugars ◽

Liquid Hot Water Pretreatment ◽

Liquid Hot Water ◽

Water Pretreatment ◽

Hot Water Pretreatment

In this work, liquid hot water pretreatment (autohydrolysis) was used to improve enzymatic hydrolysis of a commonly consumed vegetable waste in Spain, Italian green pepper, to finally produce fermentable sugars. Firstly, the effect of temperature and contact time on sugar recovery during pretreatment (in insoluble solid and liquid fraction) was studied in detail. Then, enzymatic hydrolysis using commercial cellulase was performed with the insoluble solid resulting from pretreatment. The objective was to compare results with and without pretreatment. The results showed that the pretreatment step was effective to facilitate the sugars release in enzymatic hydrolysis, increasing the global sugar yield. This was especially notable when pretreatment was carried out at 180 °C for 40 min for glucose yields. In these conditions a global glucose yield of 61.02% was obtained. In addition, very low concentrations of phenolic compounds (ranging from 69.12 to 82.24 mg/L) were found in the liquid fraction from enzymatic hydrolysis, decreasing the possibility of fermentation inhibition produced by these components. Results showed that Italian green pepper is an interesting feedstock to obtain free sugars and prevent the enormous quantity of this food waste discarded annually.

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