scholarly journals Silver nanoparticles (AgNPs) from plant extracts: جسيمات الفضة متناهية الصغر من مستخلصات النبات

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Samiyah Saeed Al-Zahrani
Keyword(s):  
Silver Nanoparticles ◽  
Plant Extracts ◽  
Pathogenic Bacteria ◽  
Pathogenic Fungi ◽  
Biological Synthesis ◽  
Toxigenic Fungi ◽  
Antimicrobial Efficiency ◽  
Wide Range ◽  
Biological Sources ◽  
And Control

    Synthesis of silver nanoparticles have achieved a distinct focus with an emphasis on their biosynthesis using biological sources such as plants and microorganisms. since plant extracts provide best opportunity for improving green nanotechnology by creating a large array of nanoparticles with antimicrobial efficiency against a wide range of microorganisms such as pathogenic bacteria and plant pathogenic fungi, numerous studies have discussed the deep inroad of antibacterial effect of AgNPs. While there is a lack of studies that advent AgNPs efficiency against toxigenic fungi and control mycotoxins that associated with fungal contamination of food products. This review aimed to summarize the biological synthesis of AgNPs from different natural sources focusing on using this approach as antifungal agent against the most important toxigenic fungi to resolve the mycotoxins evolving issues which influence human and animals’ lives.    

scholarly journals Aspects regarding the effectiveness of volatile oils of the Tanacetum vulgare kind in the conservation of heritage objects

2020 ◽  
Author(s):  
Lucretia Miu ◽  
Rodica Roxana Constantinescu ◽  
Alpaslan Durmus Kaya ◽  
Emanuel Hadimbu ◽  
Claudiu Sendrea ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Antibacterial Properties ◽  
Pathogenic Fungi ◽  
Specific Test ◽  
Tanacetum Vulgare ◽  
Test Standards ◽  
Antimicrobial Efficiency ◽  
Wide Range ◽  
Microbial Strains ◽  
Logarithmic Reduction

The paper refers to a composition with antifungal and antibacterial effect in order to test new materials for preserving heritage objects on collagen support with bactericidal/antifungal role, essential oils-based from Vetrice (Tanacetum vulgare, Compositae family), having antifungal and antibacterial properties. The biocidal effect of plant extracts is due to the presence of constituent agents, such as alcohols, ethers, phenols, aldehydes, ketones, which makes them extremely effective against a wide range of microbial strains. The essential oil was obtained by boiling the plants through continuous hydrodistillation with Clevenger refrigerant. The testing of the antimicrobial efficiency of the plant extracts was performed on two strains of pathogenic fungi, respectively Trichophyton interdigitale and Epidermophyton floccosum. The microbial inoculum was mixed with the plant extract from various sources (leaves, flowers and mixed flower-leaves). All extracts were tested in duplicate according to the specific test standards, and the results were expressed as a mean percentage and logarithmic reduction between the readings on the two Petri plates corresponding to each sample. To quantify the antimicrobial efficacy, the degree of microbial and logarithmic reduction of each sample was calculated, relative to the initial cell concentration. The results of antimicrobial tests showed a high antifungal character of the extracts obtained from flowers, leaves and mixed flower-leaves.

scholarly journals Isolation and Characterization of Bacteriophage ZCSE6 against Salmonella spp.: Phage Application in Milk

Biologics ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 164-176
Author(s):  
Abdallah S. Abdelsattar ◽  
Anan Safwat ◽  
Rana Nofal ◽  
Amera Elsayed ◽  
Salsabil Makky ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Raw Milk ◽  
Salmonella Spp ◽  
Isolation And Characterization ◽  
Food Borne ◽  
Wide Range ◽  
Almost All ◽  
And Control ◽  
Milk And Dairy Products

Food safety is very important in the food industry as most pathogenic bacteria can cause food-borne diseases and negatively affect public health. In the milk industry, contamination with Salmonella has always been a challenge, but the risks have dramatically increased as almost all bacteria now show resistance to a wide range of commercial antibiotics. This study aimed to isolate a bacteriophage to be used as a bactericidal agent against Salmonella in milk and dairy products. Here, phage ZCSE6 has been isolated from raw milk sample sand molecularly and chemically characterized. At different multiplicities of infection (MOIs) of 0.1, 0.01, and 0.001, the phage–Salmonella interaction was studied for 6 h at 37 °C and 24 h at 8 °C. In addition, ZCSE6 was tested against Salmonella contamination in milk to examine its lytic activity for 3 h at 37 °C. The results showed that ZCSE6 has a small genome size (<48.5 kbp) and belongs to the Siphovirus family. Phage ZCSE6 revealed a high thermal and pH stability at various conditions that mimic milk manufacturing and supply chain conditions. It also demonstrated a significant reduction in Salmonella concentration in media at various MOIs, with higher bacterial eradication at higher MOI. Moreover, it significantly reduced Salmonella growth (MOI 1) in milk, manifesting a 1000-fold decrease in bacteria concentration following 3 h incubation at 37 °C. The results highlighted the strong ability of ZCSE6 to kill Salmonella and control its growth in milk. Thus, ZCSE6 is recommended as a biocontrol agent in milk to limit bacterial growth and increase the milk shelf-life.

scholarly journals Screening, Identification and Efficacy Evaluation of Antagonistic Bacteria for Biocontrol of Soft Rot Disease Caused by Dickeya zeae

2020 ◽  
Vol 8 (5) ◽  
pp. 697 ◽  
Author(s):  
Jieling Li ◽  
Ming Hu ◽  
Yang Xue ◽  
Xia Chen ◽  
Guangtao Lu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Prevention And Control ◽  
Pathogenic Bacteria ◽  
Soft Rot ◽  
Antagonistic Bacteria ◽  
Bacterial Soft Rot ◽  
Soft Rot Disease ◽  
Wide Range ◽  
Rot Disease ◽  
And Control

Dickeya zeae is the causal agent of bacterial soft rot disease, with a wide range of hosts all over the world. At present, chemical agents, especially agricultural antibiotics, are commonly used in the prevention and control of bacterial soft rot, causing the emergence of resistant pathogens and therefore increasing the difficulty of disease prevention and control. This study aims to provide a safer and more effective biocontrol method for soft rot disease caused by D. zeae. The spot-on-lawn assay was used to screen antagonistic bacteria, and three strains including SC3, SC11 and 3-10 revealed strong antagonistic effects and were identified as Pseudomonas fluorescens, P. parafulva and Bacillus velezensis, respectively, using multi-locus sequence analysis (MLSA) based on the sequences of 16S rRNA and other housekeeping genes. In vitro antimicrobial activity showed that two Pseudomonas strains SC3 and SC11 were only antagonistic to some pathogenic bacteria, while strain 3-10 had broad-spectrum antimicrobial activity on both pathogenic bacteria and fungi. Evaluation of control efficacy in greenhouse trials showed that they all restrained the occurrence and development of soft rot disease caused by D. zeae MS2 or EC1. Among them, strain SC3 had the most impressive biocontrol efficacy on alleviating the soft rot symptoms on both monocotyledonous and dicotyledonous hosts, and strain 3-10 additionally reduced the occurrence of banana wilt disease caused by Fusarium oxysporum f. sp. cubensis. This is the first report of P. fluorescens, P. parafulva and B. velezensis as potential bio-reagents on controlling soft rot disease caused by D. zeae.

scholarly journals Biosynthesized silver nanoparticles using Ulva lactuca as a safe synthetic pesticide (in vitro)

2020 ◽  
Vol 5 (1) ◽  
pp. 291-299
Author(s):  
Hadear Hanie Amin
Keyword(s):  
Silver Nanoparticles ◽  
Ulva Lactuca ◽  
Biological Synthesis ◽  
Agricultural Crops ◽  
Ag Nps ◽  
Wide Range ◽  
Synthetic Pesticide ◽  
Ft Ir ◽  
Bacteria And Fungi

AbstractIn this era, we must synthesize safe pesticides from inexpensive sources to avoid the diseases caused by most of the previously used pesticides. Therefore, nanotechnology was used to produce biologically synthesis pesticides from very cheap sources such as seaweed, especially green algae, as it is safe to synthesize a pesticide against various pests such as bacteria and fungi that affect various agricultural crops. Ulva lactuca is used for the biosynthesis of silver nanoparticles by a bottom-up bioreduction reaction of silver nitrate to silver nanoparticles (Ag-NPs) observed by the formation of brown color. The biosynthesis reaction has been proven by using UV-VIS, FT-IR, EDAX, SEM, TEM, and DSC-TGA and has been tested against a wide range of bacteria and fungi that affect plants, poultry, fish, rabbits, animals, and humans. Antioxidant activity was also determined. Silver nanoparticles (Ag-NPs) have proven to be good and safe synthetic pesticides. The results of the spectroscopy demonstrated the success of the biological synthesis of the pesticide. Also, the results of the antimicrobial activity demonstrated the success of the pesticide that was biologically synthesized to fight bacteria and fungi that cause different diseases of different agricultural crops and should be used as a safe synthetic pesticide.

scholarly journals Review of Biosynthesis Silver Nanoparticles by Microbiology

2020 ◽  
Vol 1 (01) ◽  
pp. 7-11
Author(s):  
Dr. Hawraa Natiq Kabroot Al-Fatlawy ◽  
Khwla Abd Al Kadhem Rahi ◽  
Diaa Sarhan Abd Al Zahra ◽  
Samara Rahem Hudud ◽  
Hussein Ali Hussein ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Endophytic Bacterium ◽  
Antibacterial Activities ◽  
Salmonella Typhi ◽  
Biological Synthesis ◽  
Klebsiella Pneumonia ◽  
Ambient Conditions ◽  
Replication Process ◽  
Synthesis Of Nanoparticles

Nanotechnology is a multidisciplinary field that evolved within the past few decades and played a substantial role in the environment, industry, agriculture, and pharmacology. Nanoparticles are generally classified based on their dimensionality, morphology, composition, uniformity, and agglomeration. The shape, and morphology of nanoparticles play an essential role in their functionality and toxic effect on the environment and humans. In this review, we discuss the biosynthesis of nanoparticles from microbes. For the biological synthesis of nanoparticles, microbes have been exploited all over the globe. Microbes like bacteria, fungi, and yeasts are mostly preferred for nanoparticles (NPs) synthesis because of their fast growth rate, easy cultivation, and their ability to grow at ambient conditions of temperature, pH, and pressure. Applications of Nanoparticles is a field of research with tremendous prospects for the improvement of the diagnosis and treatment of human diseases. Microbial nanoparticles are found to have vigorous antibacterial activities. The nanoparticles' efficiency is probably due to their larger surface area for enhanced interaction with the micro-organisms. Nanoparticles adhere to the cell membrane and further penetrate inside by interacting with DNA, thereby interfering with the replication process or may attack the respiratory chain of pathogens. A similar bactericidal mechanism of silver nanoparticles obtained from endophytic bacterium Bacillus cereus was observed against pathogenic bacteria like Salmonella typhi, Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Pseudomonas aeruginosa.

scholarly journals Silver nanoparticles: synthesis, characterisation and biomedical applications

2020 ◽  
Vol 15 (1) ◽  
pp. 819-839
Author(s):  
Ahmad Almatroudi
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Synthesis Methods ◽  
Medical Treatments ◽  
Antitumour Agents ◽  
Anticancer Properties ◽  
Wide Range ◽  
Biological Methods ◽  
Antibiofilm Agents ◽  
And Control

AbstractNanotechnology is a rapidly growing field due to its unique functionality and a wide range of applications. Nanomedicine explores the possibilities of applying the knowledge and tools of nanotechnology for the prevention, treatment, diagnosis and control of disease. In this regard, silver nanoparticles with diameters ranging from 1 to 100 nm are considered most important due to their unique properties, ability to form diverse nanostructures, their extraordinary range of bactericidal and anticancer properties, wound healing and other therapeutic abilities and their cost-effectiveness in production. The current paper reviews various types of physical, chemical and biological methods used in the production of silver nanoparticles. It also describes approaches employing silver nanoparticles as antimicrobial and antibiofilm agents, as antitumour agents, in dentistry and dental implants, as promoters of bone healing, in cardiovascular implants and as promoters of wound healing. The paper also explores the mechanism of action, synthesis methods and morphological characterisation of silver nanoparticles to examine their role in medical treatments and disease management.

scholarly journals Biosynthesis of Silver Nanoparticles from Date (Phoenix dactylifera) Seeds Extract and Evaluation of Antibacterial Activity Against Pathogenic Bacteria

2020 ◽  
Vol 36 (6) ◽  
pp. 1189-1193
Author(s):  
Saleh H. Salmen
Keyword(s):  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Date Palm ◽  
Antibacterial Effect ◽  
Phoenix Dactylifera ◽  
Seed Extract ◽  
Biological Synthesis ◽  
Kingdom Of Saudi Arabia ◽  
Date Seed ◽  
Transmission Electron

Interest in silver nanoparticles (AgNPs) has recently increased due to their extensive and varied use, the biological synthesis of synthesis of AgNPs being safer than other approaches. The Kingdom of Saudi Arabia is one of the biggest producers of date palm, leading to the production of large amounts of waste date seeds. Here, the study evaluatesthe use of date seeds extract in the biosynthesis of AgNPs and to evaluate the antibacterial effect of the resultant nanoparticles (NPs) against pathogenic bacteria. A number of which were used here in order to characterize the synthesized particles, including UV-Vis absorption spectroscopy, transmission electron microscopy (TEM) and energy dispersive spectroscopy (EDS). The results show that date seed extract can be used to synthesize AgNPs effectively. These AgNPs exhibit unique, small-size morphologies which proved to be antibacterial against pathogenic bacteria including Staphylococcus aureus, S. epidermidis, and Escherichia coli.

scholarly journals Synthesis and Characterization of Silver Nanoparticles Using Prodigiosin Pigment and Evaluation of Their Antibacterial and Anti-Inflammatory Activities

2021 ◽  
pp. 1103-1120
Author(s):  
Oulan Alaa ◽  
Nadham Hassan
Keyword(s):  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Ion Concentration ◽  
Biological Synthesis ◽  
Ag Nps ◽  
Optimum Synthesis ◽  
A Value ◽  
Anti Inflammatory ◽  
Temperature Time

This study focused on the biological synthesis of silver nanoparticles (AgNPs), using prodigiosin pigment produced by Serratia marcescens. The effect of parameters such as pH, temperature, time, with various concentrations of silver nitrate (AgNO3) and prodigiosin on the synthesis of AgNPs were also studied.  Optimized results of the biosynthesis process revealed an increase in the intensity of Surface Plasmon Resonance (SPR) bands of nanoparticles with shifting at the wavelength of 400 nm. In addition, optimum synthesis of AgNPs was achieved at pH 12, temperature 55℃, and reaction time 24 h, with concentrations of prodigiosin, as a reducing agent, of 12.5 µg/ml and silver ion concentration of 1 mM. Measurement of the size of silver nanoparticles by SEM diffraction revealed a value of 30 nm. Finally, the minimum inhibitory concentration of AgNPs against pathogenic bacteria was 32 µg/ml for Staphylococcus aureus and Pseudomonas aeruginosa. The results of anti-inflammatory effects of Ag NPs obviously demonstrated that the infections of test animals treated with AgNPs were completely healed after 4 days of treatment, while the animals treated with fucidin (as control) did not exhibit any healing.

Experimental Study of the Impact of Alisma plantago-aquatica Secretions on Pathogenic Bacteria

2014 ◽  
Vol 1 (3) ◽  
pp. 3-7
Author(s):  
O. Zhukorskyy ◽  
O. Hulay
Keyword(s):  
Pathogenic Bacteria ◽  
Initial Density ◽  
Biologically Active ◽  
Erysipelothrix Rhusiopathiae ◽  
Natural Focus ◽  
Test Species ◽  
Popula Tions ◽  
And Control ◽  
The Impact

Aim. To estimate the impact of in vivo secretions of water plantain (Alisma plantago-aquatica) on the popula- tions of pathogenic bacteria Erysipelothrix rhusiopathiae. Methods. The plants were isolated from their natural conditions, the roots were washed from the substrate residues and cultivated in laboratory conditions for 10 days to heal the damage. Then the water was changed; seven days later the selected samples were sterilized using fi lters with 0.2 μm pore diameter. The dilution of water plantain root diffusates in the experimental samples was 1:10–1:10,000. The initial density of E. rhusiopathiae bacteria populations was the same for both experimental and control samples. The estimation of the results was conducted 48 hours later. Results. When the dilution of root diffusates was 1:10, the density of erysipelothrixes in the experimental samples was 11.26 times higher than that of the control, on average, the dilution of 1:100 − 6.16 times higher, 1:1000 – 3.22 times higher, 1:10,000 – 1.81 times higher, respectively. Conclusions. The plants of A. plantago-aquatica species are capable of affecting the populations of E. rhusiopathiae pathogenic bacteria via the secretion of biologically active substances into the environment. The consequences of this interaction are positive for the abovementioned bacteria, which is demon- strated by the increase in the density of their populations in the experiment compared to the control. The intensity of the stimulating effect on the populations of E. rhusiopathiae in the root diffusates of A. plantago-aquatica is re- ciprocally dependent on the degree of their dilution. The investigated impact of water plantain on erysipelothrixes should be related to the topical type of biocenotic connections, the formation of which between the test species in the ecosystems might promote maintaining the potential of natural focus of rabies. Keywords: Alisma plantago-aquatica, in vivo secretions, Erysipelothrix rhusiopathiae, population density, topical type of connections.

THE SOME QUESTIONS OF AUTONOMOUS MOTION AND MANAGEMENT OF LAND-MOBILE ROBOTIC COMPLEXES FOR UKRAINIAN GROUND FORCESE

2019 ◽  
pp. 53-58
Author(s):  
О. Кravchuk ◽  
V. Symonenkov ◽  
I. Symonenkova ◽  
O. Hryhorev
Keyword(s):  
Large Scale ◽  
Armed Forces ◽  
Distributed Information ◽  
Wide Range ◽  
Highly Effective ◽  
Information Management Systems ◽  
Robotic Devices ◽  
Principles And Standards ◽  
And Control ◽  
The Eu

Today, more than forty countries of the world are engaged in the development of military-purpose robots. A number of unique mobile robots with a wide range of capabilities are already being used by combat and intelligence units of the Armed forces of the developed world countries to conduct battlefield intelligence and support tactical groups. At present, the issue of using the latest information technology in the field of military robotics is thoroughly investigated, and the creation of highly effective information management systems in the land-mobile robotic complexes has acquired a new phase associated with the use of distributed information and sensory systems and consists in the transition from application of separate sensors and devices to the construction of modular information subsystems, which provide the availability of various data sources and complex methods of information processing. The purpose of the article is to investigate the ways to increase the autonomy of the land-mobile robotic complexes using in a non-deterministic conditions of modern combat. Relevance of researches is connected with the necessity of creation of highly effective information and control systems in the perspective robotic means for the needs of Land Forces of Ukraine. The development of the Armed Forces of Ukraine management system based on the criteria adopted by the EU and NATO member states is one of the main directions of increasing the effectiveness of the use of forces (forces), which involves achieving the principles and standards necessary for Ukraine to become a member of the EU and NATO. The inherent features of achieving these criteria will be the transition to a reduction of tasks of the combined-arms units and the large-scale use of high-precision weapons and land remote-controlled robotic devices. According to the views of the leading specialists in the field of robotics, the automation of information subsystems and components of the land-mobile robotic complexes can increase safety, reliability, error-tolerance and the effectiveness of the use of robotic means by standardizing the necessary actions with minimal human intervention, that is, a significant increase in the autonomy of the land-mobile robotic complexes for the needs of Land Forces of Ukraine.

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