Applications Of Silver Nanoparticles In Dentistry

Nanotechnology is a booming field of research and innovation that aims at building materials on the scale of atoms and molecules. Essentially Nanotechnology is characterized as the plan, characterisation, creation and utilization of structures, gadgets and frameworks by controlled control of size and shape at the nanometer scale. It is a booming field of this 21st century. Silver Nanoparticles are known for their various physical, chemical and quantum properties that make them unique. They have got excellent antimicrobial properties that extend their applications nearly in every sphere of life. Apart from the antimicrobial property, they show excellence in their Anti-inflammatory and Anti-oxidation properties. Silver nanoparticles also have many optical, mechanical, biological and chemical properties that attribute to their enhanced performances in the evaluation and clinical assessments of mechanical devices and other biomaterials. Uses of Silver Nanoparticles in the field of dentistry is remarkable. Silver nanoparticles can be used in association with dental acrylic resins, intracanal medication and in implant coatings. The current study aims at discussing the applications of silver nanoparticles in various aspects of dentistry.

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Antimicrobial properties of acrylic resins for dentures impregnated with silver nanoparticles

Acta stomatologica Naissi ◽

10.5937/asn1775696g ◽

2017 ◽

Vol 33 (75) ◽

pp. 1696-1702 ◽

Cited By ~ 1

Author(s):

Nikola Gligorijevic ◽

Milena Kostic ◽

Ana Tacic ◽

Ljubisa Nikolic ◽

Vesna Nikolic

Keyword(s):

Silver Nanoparticles ◽

Antimicrobial Properties ◽

Acrylic Resins

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Coconut inflorescence sap mediated synthesis of silver nanoparticles and its diverse antimicrobial properties

10.1101/775940 ◽

2019 ◽

Author(s):

M.K. Rajesh ◽

K.S. Muralikrishna ◽

Swapna S. Nair ◽

B. Krishna Kumar ◽

T.M. Subrahmanya ◽

...

Keyword(s):

Electron Microscopy ◽

Silver Nanoparticles ◽

Secondary Metabolites ◽

Hela Cells ◽

Pathogenic Bacteria ◽

Incubation Temperature ◽

Antimicrobial Property ◽

Antimicrobial Properties ◽

Biological Origin ◽

Synthesis Of Nanoparticles

ABSTRACTGreen synthesis of nanoparticles (NPs) involves the use of diverse extracts of biological origin as substrates to synthesize nanoparticles and can overcome the hazards associated with chemical methods. Coconut inflorescence sap, which is unfermented phloem sap obtained by tapping of coconut inflorescence, is a rich source of sugars and secondary metabolites. In this study, coconut inflorescence sap was used to synthesize silver nanoparticles (AgNPs). We have initially undertaken metabolomic profiling of coconut inflorescence sap from West Coast Tall cultivar to delineate its individual components. Secondary metabolites constituted the major portion of the inflorescence sap along with sugars, lipids and, peptides. The concentration of silver nitrate, inflorescence sap and incubation temperature for synthesis of AgNPs were optimized. Incubating the reaction mixture at 40°C was found to enhance AgNP synthesis. The AgNPs synthesized were characterized using UV-Visible spectrophotometry, X-Ray Diffraction (XRD), Fourier Transform Infrared spectroscopy (FTIR), Field Emission Scanning Electron Microscopy (FESEM) and Transmission Electron Microscopy (TEM). Antimicrobial property of AgNP was tested in tissue culture of arecanut (Areca catechu L.) where bacterial contamination (Bacillus pumilus) was a frequent occurrence. Significant reduction in the contamination was observed when plantlets were treated with aqueous solutions of 0.01, 0.02 and 0.03% of AgNPs for one hour. Notably, treatment with AgNPs did not affect growth and development of the arecanut plantlets. Cytotoxicity of AgNPs was quantified in HeLa cells. Viability (%) of HeLa cells declined significantly at 10 ppm concentration of AgNP and complete mortality was observed at 60 ppm. Antimicrobial properties of AgNPs synthesized from inflorescence sap were also evaluated and confirmed in human pathogenic bacteria viz., Salmonella sp., Vibrio parahaemolyticus, and Escherichia coli. The study concludes that unfermented inflorescence sap, with above neutral pH, serves as an excellent reducing agent to synthesize AgNPs from Ag+. Graphical abstract

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A Study of Antimicrobial Property of Oxidized Cotton Fabric Finished with Amino-Terminated Hyperbranched Polymer

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.175-176.640 ◽

2011 ◽

Vol 175-176 ◽

pp. 640-645

Author(s):

Ling Chen ◽

De Suo Zhang ◽

Hong Lin ◽

Yu Yue Chen

Keyword(s):

Silver Nanoparticles ◽

Antimicrobial Agent ◽

Cotton Fabric ◽

Hyperbranched Polymer ◽

Molecular Conformation ◽

Antimicrobial Property ◽

Nitrate Solution ◽

Antimicrobial Properties ◽

Molecular Structures ◽

Colloid Solution

The chief aim of the present work is to investigate the preparation for silver nanoparticles antimicrobial agent and its antibacterial activity on cotton fabric. In this study, antimicrobial agent was fabricated by a reaction between an amino-terminated hyperbranched polymer (HBP-NH2) and silver nitrate solution of certain concentration. Meanwhile, cotton fabric was oxygenated to afford aldehyde groups which could connect with the amino groups of the HBP-NH2 to provide cotton fabric with durable antimicrobial properties. The effects of different biocides made of various molecular structures of synthetic material (HBP-NH2) on antimicrobial properties of nano-silver colloid solution were discussed, and three different agents generated then were characterized in following aspects as silver nanoparticles size and distribution by using DLS, TEM and UV-vis. Furthermore, the fabric structure, mechanical properties and antimicrobial property of treated cotton fabric were also tested. Included in this part of experiment were transverse micro morphology of cotton fibre by SEM, fabric strength retention after finishing, silver nanoparticles molecular conformation on fabric and content analysis via ICP-AES. The results showed that stable silver nanoparticles collide solution with 20-30 nano, applied on oxidized cotton fabric under certain condition, could produce ideal antibacterial rate over 94% of bacterial reduction to both Staphylococcus aureus (S.aureus) and Escherichia coli (E.coli) after 50 consecutive washings.

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Silver Nanoparticles and Mitochondrial Interaction

International Journal of Dentistry ◽

10.1155/2013/312747 ◽

2013 ◽

Vol 2013 ◽

pp. 1-8 ◽

Cited By ~ 42

Author(s):

Eriberto Bressan ◽

Letizia Ferroni ◽

Chiara Gardin ◽

Chiara Rigo ◽

Michele Stocchero ◽

...

Keyword(s):

Silver Nanoparticles ◽

High Surface ◽

Specific Interaction ◽

Antimicrobial Properties ◽

Chemical Properties ◽

Volume Ratio ◽

Clear Understanding ◽

Different Types ◽

Physical And Chemical ◽

And Chemical Properties

Nanotechnology has gone through a period of rapid growth, thus leading to the constant increase in the application of engineered nanomaterials in daily life. Several different types of nanoparticles have been engineered to be employed in a wide array of applications due to their high surface to volume ratio that leads to unique physical and chemical properties. So far, silver nanoparticles (AgNps) have been used in many more different medical devices than any other nanomaterial, mainly due to their antimicrobial properties. Despite the promising advantages posed by using AgNps in medical applications, the possible health effects associated with the inevitable human exposure to AgNps have raised concerns as to their use since a clear understanding of their specific interaction with biological systems has not been attained yet. In light of such consideration, aim of the present work is the morphological analysis of the intracellular behavior of AgNps with a diameter of 10 nm, with a special attention to their interaction with mitochondria.

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Coating-Dependent Effects of Silver Nanoparticles on Tobacco Seed Germination and Early Growth

International Journal of Molecular Sciences ◽

10.3390/ijms21103441 ◽

2020 ◽

Vol 21 (10) ◽

pp. 3441 ◽

Cited By ~ 1

Author(s):

Renata Biba ◽

Dajana Matić ◽

Daniel Mark Lyons ◽

Petra Peharec Štefanić ◽

Petra Cvjetko ◽

...

Keyword(s):

Silver Nanoparticles ◽

Growth Parameters ◽

Antimicrobial Properties ◽

Chemical Properties ◽

Early Growth ◽

Consumer Products ◽

Negative Effects ◽

Wide Range ◽

Living Organisms ◽

The Stability

Silver nanoparticles (AgNPs) are used in a wide range of consumer products because of their excellent antimicrobial properties. AgNPs released into the environment are prone to transformations such as aggregation, oxidation, or dissolution so they are often stabilised by coatings that affect their physico-chemical properties and change their effect on living organisms. In this study we investigated the stability of polyvinylpyrrolidone (PVP) and cetyltrimethylammonium bromide (CTAB) coated AgNPs in an exposure medium, as well as their effect on tobacco germination and early growth. AgNP-CTAB was found to be more stable in the solid Murashige and Skoog (MS) medium compared to AgNP-PVP. The uptake and accumulation of silver in seedlings was equally efficient after exposure to both types of AgNPs. However, AgNP-PVP induced only mild toxicity on seedlings growth, while AgNP-CTAB caused severe negative effects on all parameters, even compared to AgNO3. Moreover, CTAB coating itself exerted negative effects on growth. Cysteine addition generally alleviated AgNP-PVP-induced negative effects, while it failed to improve germination and growth parameters after exposure to AgNP-CTAB. These results suggest that the toxic effects of AgNP-PVP are mainly a consequence of release of Ag+ ions, while phytotoxicity of AgNP-CTAB can rather be ascribed to surface coating itself.

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Chemical and Physical Analyses of Synthesized Silver Nanoparticles

Vanderbilt Undergraduate Research Journal ◽

10.15695/vurj.v9i0.3794 ◽

2013 ◽

Vol 9 ◽

Cited By ~ 1

Author(s):

Kavya Sharman

Keyword(s):

Silver Nanoparticles ◽

Antimicrobial Property ◽

Antimicrobial Properties ◽

Physical Analysis ◽

Significance Level ◽

Bacterial Inhibition ◽

Physical Chemical ◽

Elemental Silver ◽

Visual Color ◽

The Relationship

Although silver has long been used as an antimicrobial agent, it is not used often due to economical and environmental concerns. However, silver nanoparticles (AgNPs) have the same properties as elemental silver and can be synthesized on a considerably more economical level. The objective of this study was to synthesize AgNPs and analyze their physical, chemical, and antimicrobial properties. The first portion of this study consisted of synthesizing AgNPs of 5nm, 35nm, 40nm, and 60nm lengths, using diluted lab solutions and sterile techniques. The physical analysis of the AgNPs was performed through visual color comparisons of their varied sizes, which confirmed their successful synthesis. A chemical analysis was then conducted using ultraviolet-visible spectroscopy, which measured their absorbance at a 635nm wavelength. Finally, the antimicrobial property of the AgNPs was investigated by inoculating a strain of Staphylococcus aureus with titrated concentrations of varied nanoparticle size. This titration allowed for the analysis of the relationship between the size of AgNPs and level of inhibition of S. aureus. The results of the bacterial inhibition were quantified using a colorimeter, which determined the density of the bacteria and the consequent level of inhibition. The results were analyzed using an ANOVA test, which determined that at a 95% significance level, AgNPs were successfully able to inhibit the growth of S. aureus. However, the results of an ensuing series of t-tests indicated that there was not a consistent relationship between the size of the nanoparticles and the level of bacterial inhibition.

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Current Research on Silver Nanoparticles: Synthesis, Characterization, and Applications

Journal of Nanomaterials ◽

10.1155/2021/6687290 ◽

2021 ◽

Vol 2021 ◽

pp. 1-23

Author(s):

Sonika Dawadi ◽

Saurav Katuwal ◽

Aakash Gupta ◽

Uttam Lamichhane ◽

Ranjita Thapa ◽

...

Keyword(s):

Silver Nanoparticles ◽

Targeted Delivery ◽

Bulk Composition ◽

Antimicrobial Properties ◽

Chemical Properties ◽

Surface Enhanced Raman Spectroscopy ◽

Photochemical Reactions ◽

Biological Synthesis ◽

Minimal Risk ◽

Ag Nps

Over the past couple of decades, nanomaterials have advanced the research in materials; biomedical, biological, and chemical sciences; etc., owing to their peculiar properties at the nanoregime compared to their bulk composition. Applications of nanoparticles in the fields like medicine and agriculture have been boosted due to the development of different methodologies developed to synthesize specific shapes and sizes. Silver nanoparticles have tunable physical and chemical properties, so it has been studied widely to improve its applicability. The antimicrobial properties of Ag NPs are finding their application in enhancing the activity of drugs (like Amphotericin B, Nystatin, Fluconazole) and composite scaffolds for controlled release of drugs and targeted delivery of drugs due to their low toxicity and biocompatibility. Similarly, their surface plasmon resonance property makes Ag NPs a top-notch material for developing (bio)sensors, for instance, in surface-enhanced Raman spectroscopy, for detecting biomarkers, diseases, pollutants, and higher catalytic activity in photochemical reactions. Besides these, highly conducting Ag NPs are used in wearable and flexible sensors to generate electrocardiographs. Physicochemical or biological approaches are used to prepare Ag NPs; however, each method has its pros and cons. The prohibitive cost and use of hazardous chemicals hinder the application of physicochemical synthesis. Likewise, biological synthesis is not always reproducible for extensive use but can be a suitable candidate for therapeutic activities like cancer therapy. Excess use of Ag NPs is cytotoxic, and their unregulated discharge in the environment may have effects on both aquatic and terrestrial biota. The research in Ag NPs has always been driven by the need to develop a technology with potential benefits and minimal risk to environmental and human health. In this review, we have attempted to provide an insight into the application of Ag NPs in various sectors along with the recent synthetic and characterization techniques used for Ag NPs.

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Efficacy of Silver Nanoparticles Synthesized from Hymenocallis species (Spider Lilly) Leaf Extract as Antimicrobial Agents

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2019.12.5.4 ◽

2019 ◽

Vol 12 (5) ◽

pp. 4644-4647

Author(s):

K.K. Gupta ◽

Neha Kumari ◽

Neha Sinha ◽

Akruti Gupta

Keyword(s):

Silver Nanoparticles ◽

Leaf Extract ◽

Antimicrobial Agents ◽

Color Change ◽

Bacterial Species ◽

Antimicrobial Property ◽

Biogenic Synthesis ◽

E Coli ◽

Light Yellow ◽

Antibiotic Property

Biogenic synthesis of silver nanoparticles synthesized from Hymenocallis species (Spider Lilly) leaf extract was subjected for investigation of its antimicrobial property against four bacterial species (E. coli, Salmonella sp., Streptococcus sp. & Staphylococcus sp.). The results revealed that synthesized nanoparticles solution very much justify the color change property from initial light yellow to final reddish brown during the synthesis producing a characteristics absorption peak in the range of 434-466 nm. As antimicrobial agents, their efficacy was evaluated by analysis of variance in between the species and among the different concentration of AgNPs solution, which clearly showed that there was significant variation in the antibiotic property between the four different concentrations of AgNPs solution and also among four different species of bacteria taken under studies. However, silver nanoparticles solution of 1: 9 and 1:4 were proved comparatively more efficient as antimicrobial agents against four species of bacteria.

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Silver Nanoparticles Stabilized with Phosphorus-Containing Heterocyclic Surfactants: Synthesis, Physico-Chemical Properties, and Biological Activity Determination

Nanomaterials ◽

10.3390/nano11081883 ◽

2021 ◽

Vol 11 (8) ◽

pp. 1883

Author(s):

Martin Pisárčik ◽

Miloš Lukáč ◽

Josef Jampílek ◽

František Bilka ◽

Andrea Bilková ◽

...

Keyword(s):

Silver Nanoparticles ◽

Biological Activity ◽

Zeta Potential ◽

Chain Length ◽

Alkyl Chain ◽

Chemical Properties ◽

Alkyl Chain Length ◽

Microbial Pathogens ◽

Physico Chemical ◽

Phosphorus Containing

Phosphorus-containing heterocyclic cationic surfactants alkyldimethylphenylphospholium bromides with the alkyl chain length 14 to 18 carbon atoms were used for the stabilization of silver nanodispersions. Zeta potential of silver nanodispersions ranges from +35 to +70 mV, which indicates the formation of stable silver nanoparticles (AgNPs). Long-chain heptadecyl and octadecyl homologs of the surfactants series provided the most intensive stabilizing effect to AgNPs, resulting in high positive zeta potential values and smaller diameter of AgNPs in the range 50–60 nm. A comparison with non-heterocyclic alkyltrimethylphosphonium surfactants of the same alkyl chain length showed better stability and more positive zeta potential values for silver nanodispersions stabilized with heterocyclic phospholium surfactants. Investigations of biological activity of phospholium-capped AgNPs are represented by the studies of antimicrobial activity and cytotoxicity. While cytotoxicity results revealed an increased level of HepG2 cell growth inhibition as compared with the cytotoxicity level of silver-free surfactant solutions, no enhanced antimicrobial action of phospholium-capped AgNPs against microbial pathogens was observed. The comparison of cytotoxicity of AgNPs stabilized with various non-heterocyclic ammonium and phosphonium surfactants shows that AgNPs capped with heterocyclic alkyldimethylphenylphospholium and non-heterocyclic triphenyl-substituted phosphonium surfactants have the highest cytotoxicity among silver nanodispersions stabilized by the series of ammonium and phosphonium surfactants.

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Silver Nanoparticles Induce Neutrophil Extracellular Traps Via Activation of PAD and Neutrophil Elastase

Biomolecules ◽

10.3390/biom11020317 ◽

2021 ◽

Vol 11 (2) ◽

pp. 317

Author(s):

HanGoo Kang ◽

Jinwon Seo ◽

Eun-Jeong Yang ◽

In-Hong Choi

Keyword(s):

Silver Nanoparticles ◽

Neutrophil Elastase ◽

Mammalian Cells ◽

Antimicrobial Properties ◽

Neutrophil Extracellular Traps ◽

Arginine Deiminase ◽

Human Neutrophils ◽

Extracellular Traps ◽

Peptidyl Arginine Deiminase ◽

Key Factor

Silver nanoparticles (AgNPs) are widely used in various fields because of their antimicrobial properties. However, many studies have reported that AgNPs can be harmful to both microorganisms and humans. Reactive oxygen species (ROS) are a key factor of cytotoxicity of AgNPs in mammalian cells and an important factor in the immune reaction of neutrophils. The immune reactions of neutrophils include the expulsion of webs of DNA surrounded by histones and granular proteins. These webs of DNA are termed neutrophil extracellular traps (NETs). NETs allow neutrophils to catch and destroy pathogens in extracellular spaces. In this study, we investigated how AgNPs stimulate neutrophils, specifically focusing on NETs. Freshly isolated human neutrophils were treated with 5 or 100 nm AgNPs. The 5 nm AgNPs induced NET formation, but the 100 nm AgNPs did not. Subsequently, we investigated the mechanism of AgNP-induced NETs using known inhibitors related to NET formation. AgNP-induced NETs were dependent on ROS, peptidyl arginine deiminase, and neutrophil elastase. The result in this study indicates that treatment of 5 nm AgNPs induce NET formation through histone citrullination by peptidyl arginine deiminase and histone cleavage by neutrophil elastase.

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