Antimicrobial potential of Polish propolis ethanol extracts towards Staphylococcus epidermidis biofilm

2020 ◽  
Author(s):  
Katarzyna Grecka
Keyword(s):  
Staphylococcus Epidermidis ◽  
Antimicrobial Potential ◽  
Ethanol Extracts

scholarly journals Antimicrobial evaluation of flowering stalks of Cistanche violacea, a holoparasitic plant collected from arid region in Qassim, Saudi Arabia

2017 ◽  
Vol 4 (6) ◽  
pp. 239
Author(s):  
Emad Mohamed Abdallah
Keyword(s):  
Saudi Arabia ◽  
Staphylococcus Epidermidis ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Antimicrobial Properties ◽  
Bacterial Isolates ◽  
Antimicrobial Potential ◽  
Antimicrobial Evaluation ◽  
Inhibition Zones ◽  
Ethanol Extracts

Objective: Cistanche violacea has been found in Qassim region (Saudi Arabia), growing as a parasitic plant on Haloxylon salicornicum. The flowering stalk part of this plant was evaluated for antimicrobial potential against various referenced bacteria and fungi.Methods: The flowering stalk parts were collected from the field, dried, extracted by maceration in 80% methanol and 80% Ethanol, The crude extracts were examined against the tested microorganisms using agar-well diffusion method.Results: The antimicrobial testing showed that among 10 referenced bacterial isolates, only one exhibited significant susceptibility to the methanol and ethanol extracts of C. violacea, which was Staphylococcus epidermidis ATCC® 12228TM, recorded 15 and 16 mm inhibition zones, respectively. While other bacteria recorded weak or no susceptibility and no antifungal activity was detected.Conclusions: In general, the methanolic and ethanolic extracts of the flowering stalks of C. violacea exhibited weak or no antimicrobial properties except with one bacterial strain, further studies on the other biological activities are recommended as well as investigating the chemical constituents. To the best of the author’s knowledge, this is the first report evaluating the antimicrobial potential of the flowering stalks of C. violacea in Qassim area, Saudi Arabia.

scholarly journals Antibacterial Activity Test of Extracts and Fractions of Cassava Leaves (Manihot esculenta Crantz) against Clinical Isolates of Staphylococcus epidermidis and Propionibacterium acnes Causing Acne

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Resmi Mustarichie ◽  
Sulistiyaningsih Sulistyaningsih ◽  
Dudi Runadi
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Clinical Isolates ◽  
Active Fraction ◽  
Cassava Leaves ◽  
Ethanol Extracts

This study is aimed at determining antibacterial activity from ethanol extracts and the most active fraction of cassava leaves against clinical isolates of Staphylococcus epidermidis and Propionibacterium acnes. Research carried out by the experimental method involved determination of plants, extraction with maceration method, fractionation with liquid-liquid extraction, antibacterial activity testing of extracts and fractions by agar diffusion method, determination of most active fraction from the extract, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) testing of most active fraction by microdilution method. The results showed that ethanol extracts of cassava leaves had antibacterial activity against both bacteria with the most active fraction indicated by ethyl acetate. MIC values of ethyl acetate fraction against S. epidermidis were in the concentration range of 2.5%–5.0% (w/v) and against P. acnes were in the concentration range of 1.25%–2.5% (w/v). The MBC value of ethyl acetate fraction against S. epidermidis was at a concentration of 5% (w/v), while P. acnes was at a concentration of 2.5% (w/v). From the results of this study, it can be concluded that the ethanol extract of cassava leaves (Manihot esculenta Crantz) has antibacterial activity against clinical isolates of Staphylococcus epidermidis as well as on Propionibacterium acnes. The fraction with the best activity from the ethanol extract of cassava leaves to the two test bacteria was shown by ethyl acetate fraction. It is suggested that cassava leaves are possible to be developed into standardized antiacne herbal.

scholarly journals Evaluation of Antimicrobial Potential of the Marine Cyanobacterium, Rivularia mesenterica

2019 ◽  
pp. 1-11 ◽  
Author(s):  
Mirjana Skočibušić ◽  
Sandra Lacić ◽  
Zorica Rašić
Keyword(s):  
Antimicrobial Activity ◽  
Resistant Bacteria ◽  
Gram Negative Bacteria ◽  
Marine Cyanobacterium ◽  
Gram Positive ◽  
Promising Alternative ◽  
Gram Negative ◽  
Antimicrobial Potential ◽  
Ethanol Extracts ◽  
Promising Source

Background: Antibiotic resistance is becoming a pivotal concern for public health accelerating the search for new antimicrobial molecules from nature. The prevention and treatment of infectious diseases by applying products from marine organisms, especially Cyanobacteria as a potential and promising source of antimicrobial agents appears as a possible alternative. Aims: To evaluate the in vitro antimicrobial potential of different extracts derived from marine cyanobacterium Rivularia mesenterica against Gram-positive and Gram-negative bacteria, including multidrug resistant bacteria, by comparison with clinically relevant antibiotics.   Methodology: The secondary metabolites were extracted from fresh and dried cyanobacterial biomass in water and different organic solvents. Antimicrobial efficacy of different extracts was evaluated by the disc diffusion assay. Additionally, the minimum inhibitory concentrations (MIC) of the ethanol extracts obtained from fresh and dried biomass was also determined. Results: The ethanol extracts obtained from fresh and dried biomass of R. mesenterica showed significant antimicrobial activity against five Gram-positive and five antibiotic resistant Gram-negative bacteria and four fungal strains in comparison with the clinically relevant antibiotics. The inhibitory effect of the ethanol extracts was observed, with MIC values in the range 0.06 to 32.00 μg/ml against tested strains. Furthermore, the water extract was inactive against of the tested bacteria and fungi. Conclusion: These results suggest that the ethanol extracts of R. mesenterica possess potent broad spectrum of antimicrobial activity, which can serve as an interesting source for antimicrobial compounds and promising alternative to synthetic antimicrobial drugs discovery.

scholarly journals Total Phenolic Content and Antibacterial Activity of Nut Grass (Cyperus rotundus L.) Extract

2018 ◽  
Vol 1 (1) ◽  
pp. 28-36
Author(s):  
Masfria ◽  
Yade Metri Permata
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Raw Materials ◽  
Water Soluble ◽  
Cyperus Rotundus ◽  
Shigella Dysenteriae ◽  
Total Polyphenol ◽  
Ethanol Extracts

Abstract. The objectives of this research was to study the macroscopic, the microscopic and phytochemicals characterization of raw materials, total polyphenol content and the antibacterial activity of nut grass extracts (Cyperus rotundus L.) against Shigella dysenteriae, Salmonella typhimurium, Staphylococcus epidermidis, and Pseudomonas aeroginosa.  The macroscopic and the microscopic characterization of raw materials were done with the determination of the water contents, the water soluble contents, the ethanol soluble contents, total ash and acid soluble ash. The extract was prepared by percolation using n-hexane, ethanol, and ethyl acetate as solvents. Phytochemical screening was done on the raw materials as well as n-hexane, ethyl acetate, and ethanol extracts. Total phenol contents were tested with the Folin-Ciocalteu reagent method. The antibacterial activity test was done by measuring the diameter of the inhibition zones using the diffusion agar method.  The results showed that raw material retrieved 7.33% water content, 21.85% water soluble contents, 7.9% ethanol soluble contents, 1.53% total ash, and 0.67% total insoluble-acid ash. The phytochemical study showed that the nut grass contains various secondary metabolites including flavonoids, glycosides, saponins, anthraquinone glycosides, and steroid/triterpenoids. The total polyphenol contents of ethanol extracts were expressed as catechin equivalents 1.1616 mg/g extract.  All of the nut grass extract showed antibacterial activity. Ethyl acetate extract of nut grass indicated the highest antibacterial activity against Shigella dysenteriae, Salmonella typhimurium, and Pseudomonas aeroginosa. Ethanol extract was only effective against Staphylococcus epidermidis.   Keywords: Antibacterial Activity, Bacteria, Cyperus Rotundus L Extracts, Phenolic

Phytochemical Screening, Phenolic Compounds Content, Antioxidant, Anti-inflammatory, and Antimicrobial Properties of Pachylobus balsamifera Guillaum from Gabon

2018 ◽  
Vol 16 (S1) ◽  
pp. S65-S73 ◽  
Author(s):  
C. Sima-Obiang ◽  
R.L. Ngoua-Meye-Misso ◽  
G.R. Ndong-Atome ◽  
J. Privat Ondo ◽  
L.C. Obame-Engonga ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Antimicrobial Properties ◽  
Water Extract ◽  
Antioxidant Property ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Antimicrobial Potential ◽  
Anti Inflammatory ◽  
Ethanol Extracts

The goal of this work is to evaluate the antioxidant anti-inflammatory properties, the phenolic compounds content, and antimicrobial potential of water–acetone, water–ethanol, and water extracts of Pachylobus balsamifera. The phenolic compounds content was evaluated to estimate their effect on the antioxidant, anti-inflammatory, and antimicrobial potential of the plant. Antioxidant activities were examined by DPPH (2,2-diphenyl-1- picrylhydrazyl) and ABTS (2,2′-azino-bis) assays. Antiinflammatory activity was determined by the proteins denaturation inhibition method. All plant extracts were evaluated against six reference strains, eleven clinical isolates, and two fungal strains. Phenols content was highest in the water–acetone and water–ethanol extracts. The water extract showed strong anti-inflammatory effect. The water–acetone extract presented a strong antioxidant property and the highest antimicrobial activities against Pseudomonas aeruginosa, Acinetobacter baumannii, Salmonella Sp., and Neisseria meningitidis. The tested microorganisms showed sensitivity to all extracts of the plant with the exception of Escherichia coli 105182 CIP, Listeria innocua LMG 135668 BHI, Salmonella enterica, Salmonella typhi, and Neisseria gonorrhea. Our results suggest that Pachylobus balsamifera extracts contain antioxidant, anti-inflammatory, and antimicrobial properties.

scholarly journals FORMULASI DAN EFEK ANTIBAKTERI MASKER PEEL-OFF EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) TERHADAP BAKTERI Staphlococcous epidermidis

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 686
Author(s):  
Regita Daimunon ◽  
Paulina V. Y. Yamlean ◽  
Imam Jayanto
Keyword(s):  
Staphylococcus Epidermidis ◽  
Ethanol Extract ◽  
Homogeneity Test ◽  
Power Test ◽  
Cycling Test ◽  
Physical Evaluation ◽  
Before And After ◽  
Time Test ◽  
Ethanol Extracts ◽  
Muntingia Calabura

ABSTRACT Kersen leaf (Muntingia calabura L.) contains flavonoids, saponins and tannins, which could inhibit bacterial activity. This study aimed to formulate, evaluate, and to test the effectiveness of antibacterial preparations for the Kersen leaf ethanol extracts mask.  This study uses the experimental method by testing the parameters of the physical evaluation requirements for the masker. Physical evaluation of preparations included organoleptic test, homogeneity test, pH test, scattering power test, dry time test, sticky test and cycling test, all tests were carried out before and after cycling test. The results of the preparation study meet the requirements of physical evaluation before the cycling test but after the cycling test is is not in accordance to the requirements for the preparation of the peel-off mask. The antibacterial test of mask peel-off ethanol extract of Kersen's leaves on Staphylococcus epidermidis bacteria produced a moderate inhibition. So it can be concluded that the ethanol extracts of Kersen leaves at a concentration of 20%.  can be formulated as a peel-off mask that is physically stable and has moderate antibacterial activity.Keywords: Kersen, Peel-off mask, Antibacterial, Staphlococcous epidermidis ABSTRAK Daun Kersen (Muntingia calabura L.) mengandung senyawa flavonoid, saponin dan tannin yang mampu menghambat aktivitas bakteri.  Penelitian ini bertujuan untuk memformulasi, mengevaluasi, serta menguji efektivitas antibakteri sediaan masker peel-off ekstrak etanol daun Kersen. Penelitian ini menggunakan metode ekperimental dengan melakukan pengujian parameter persyaratan evaluasi fisik masker peel-off. Evaluasi fisik sediaan meliputi uji organoleptik, uji homogenitas, uji pH, uji daya sebar, uji waktu sediaan mengering, uji daya lekat dan uji cycling test semua pengujian dilakukan sebelum dan sesudah cycling test. Hasil penelitian sediaan memenuhi persyaratan eveluasi fisik sebelum uji cycling test namun setelahnya tidak sesuai dengan persyaratan sediaan masker peel-off. Penelitian uji antibakteri masker peel-off ekstrak etanol daun Kersen pada bakteri staphylococcus epidermidis menghasilkan daya hambat yang sedang. Dapat disimpulkan pada kosentrasi 20% ekstrak etanol daun Kersen dapat diformulasi sebagai sediaan masker peel-off yang stabil secara fisik dan memiliki aktivitas antibakteri yang sedang .Kata kunci : Kersen, Masker peel-off, Antibakteri, Staphlococcous  epidermidis

scholarly journals Antibacterial activity of ethanol extracts of betel leaf (Piper betle L.) and areca (Areca catechu L.) nuts against food borne and oral pathogens

2021 ◽  
Vol 38 (1) ◽  
pp. 15-19
Author(s):  
Masuda Khatun ◽  
Md Mahfuzul Hoque
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Epidermidis ◽  
Ethanol Extract ◽  
Piper Betle ◽  
Areca Nut ◽  
Betel Leaf ◽  
Food Borne ◽  
Areca Catechu ◽  
Oral Pathogens ◽  
Ethanol Extracts

Antimicrobial activity of ethanol extracts of betel leaf (Piper betle L.) and areca nut (Areca catechu) against six food borne enteric pathogens viz. Staphylococcus aureus (ATCC 25923), Staphylococcus epidermidis (icddr,b), Salmonella typhimurium (AIM-40, icddr,b), Escherichia coli (ATCC 25922), Escherichia coli O157:H7 (ATCC 12079) and Bacillus cereus (ATCC 12079), and four oral pathogens such as isolates 1 & 2 of each of the two bacteria viz. Staphylococcus sp. and Pseudomonas sp. was investigated. Ethanol extract of betel leaf produced highest zone of inhibition (18.0 ± 1.91 mm) against Staphylococcus epidermidis (icddr,b) whereas that of areca nut produced highest zone of inhibition (15.0 ± 0.816 mm) against Staphylococcus aureus (ATCC 25923). But no inhibition was found against Salmonella typhimurium (ATCC AIM-40, icddr,b), Escherichia coli (ATCC 25922), and Escherichia coli O157:H7 (ATCC 12079) by the Ethanol extract of betel leaf. The MIC and MBC values of both ethanol extracts indicated that Gram positive organisms were more susceptible than Gram negative organisms. Highest antimicrobial activity of ethanol extracts of betel leaf was found against the isolate 1 of Staphylococcus sp. (16.5±0.5) and that of areca nut was recorded against the isolate 1 of Staphylococcus sp. (12.0±0.5) and the isolate 2 of Pseudomonas sp. (12.0±0.5). Present study reveals the potentials of both the extracts to inhibit food borne enteric and oral pathogens that could be used as food preservatives to prevent the food borne illness as well as for maintaining the oral and gut health. Bangladesh J Microbiol, Volume 38, Number 1, June 2021, pp 15-19

scholarly journals Phytochemical screening and antibacterial activity of crude aqueous and ethanol extracts of Salvadora persica L. stem (Miswak) from Saudi Arabia

2015 ◽  
Vol 4 (5) ◽  
pp. 243-247
Author(s):  
Emad Mohamed Abdallah ◽  
◽  
Khalid Assaf Al Harbi ◽  
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Antimicrobial Effect ◽  
Penicillin G ◽  
Klebsiella Pneumonia ◽  
Gram Negative Bacteria ◽  
Salvadora Persica ◽  
Gram Positive Bacteria ◽  
Long Time ◽  
Ethanol Extracts

Since long time ago, medicinal plant products have been a rich source of antibacterial drugs. Salvadora persica L. stem is used basically for cleanse, get rid of microbes and the stink smell of the mouth. Phytochemical investigations on the aqueous and ethanol extracts of Salvadora persica L. stem revealed presence of some bioactive principles, such as Saponins, alkaloids, cardiac glycosides, Terpenoids and flavonoids. Antimicrobial investigation revealed presence of some degree of antimicrobial effect against some gram negative bacteria (Salmonella enterica ATCC 5174, Proteus vulgaris ATCC 49132, Klebsiella pneumonia ATCC 27736, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853) and some gram positive bacteria (Bacillus cereus ATCC 10876, Staphylococcus epidermidis ATCC 49461, Staphylococcus aureus ATCC 25923). This antibacterial activity considered Competitor to penicillin G, but modest when compared to gentamicin. The antibiotic penicillin is collapsing and it is no longer a good option.

scholarly journals Synergy between antibiotics and natural agents results in increased antimicrobial activity against Staphylococcus epidermidis

2015 ◽  
Vol 9 (09) ◽  
pp. 925-929 ◽  
Author(s):  
Syed Hani Abidi ◽  
Khalid Ahmed ◽  
Sikander Khan Sherwani ◽  
Shahana Urooj Kazmi
Keyword(s):  
Camellia Sinensis ◽  
Staphylococcus Epidermidis ◽  
Juglans Regia ◽  
Clinical Isolates ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Synergistic Activity ◽  
Disk Diffusion Method ◽  
Antimicrobial Potential ◽  
Natural Agents

Introduction: Staphylococcus epidermidis is one of the most frequent causes of biofilm-associated infections on indwelling medical devices. With the emergence of methicillin-resistant S. epidermidis (MRSE), there is an urgent need to discover novel active agents against a range of Gram-positive pathogens. We screened the clinical isolates of S. epidermidis for susceptibility/resistance against commonly prescribed antibiotics. Furthermore, we tested some natural agents alone and in combination with antibiotics to find possible synergistic antimicrobial effects. Methodology: S. epidermidis clinical isolates were screened for susceptibility/resistance against vancomycin, erythromycin, tetracycline, chloramphenicol, ampicillin, ofloxacin, cephalexin, and gentamicin using the Kirby-Bauer disk diffusion method. The antimicrobial potential of Camellia sinensis, Juglans regia, and Hippophae rhamnoides alone and in combination with antibiotics were examined using the disk diffusion method, where the antimicrobial potential activity was measured in terms of formation of zones of inhibition. Results: Most S. epidermidis isolates were found to be resistant to one or more antibiotics. Gentamycin and ofloxacin were found to be the most effective antibiotics against S. epidermidis isolates. Extracts of Hippophae rhamnoides, Juglans regia, and Camellia sinensis were found to be equally effective against S. epidermidis isolates. In combination with antibiotics, these extracts exhibited appreciable synergistic activity; the highest synergistic activity was observed with erythromycin and cephalexin. In the case of cephalexin, a reversion in resistance was observed. Conclusions: The plant extracts used in the study exhibited additive and synergistic antibacterial activity against S. epidermidis, hence providing an effective alternative to deal with the problem of multidrug resistance.

Scanning Electron Microscopy of Staphylococcus Epidermidis Adherence to Continuous Ambulatory Peritoneal Dialysis Catheters

1985 ◽  
Vol 43 ◽  
pp. 520-521
Author(s):  
William J. Lamoreaux ◽  
David L. Smalley ◽  
Larry M. Baddour ◽  
Alfred P. Kraus
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Peritoneal Dialysis ◽  
Continuous Ambulatory Peritoneal Dialysis ◽  
Staphylococcus Epidermidis ◽  
Sterile Saline ◽  
Intravascular Devices ◽  
Capd Patient ◽  
Scanning Electron

Infections associated with the use of intravascular devices have been documented and have been reported to be related to duration of catheter usage. Recently, Eaton et al. reported that Staphylococcus epidermidis may attach to silastic catheters used in continuous ambulatory peritoneal dialysis (CAPD) treatment. The following study presents findings using scanning electron microscopy (SEM) of S. epidermidis adherence to silastic catheters in an in vitro model. In addition, sections of polyvinyl chloride (PVC) dialysis bags were also evaluated by SEM.The S. epidermidis strain RP62A which had been obtained in a previous outbreak of coagulase-negative staphylococcal sepsis at local hospitals was used in these experiments. The strain produced surface slime on exposure to glucose, whereas a nonadherent variant RP62A-NA, which was also used in these studies, failed to produce slime. Strains were grown overnight on blood agar plates at 37°C, harvested from the surface and resuspended in sterile saline (0.85%), centrifuged (3,000 rpm for 10 minutes) and then washed twice in 0.1 M phosphate-buffered saline at pH 7.0. Organisms were resuspended at a concentration of ca. 106 CFU/ml in: a) sterile unused dianeal at 4.25% dextrose, b) sterile unused dianeal at 1.5% dextrose, c) sterile used dialysate previously containing 4.25% dextrose taken from a CAPD patient, and d) sterile used dialysate previously containing 1.5% dextrose taken from a CAPD patient.

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