scholarly journals Effect of electromagnetic field on whole blood, biochemical and hormone level in human

2019 ◽  
Vol 8 (2) ◽  
pp. 47-53
Author(s):  
Mehmet Cihan YAVAŞ ◽  
Veysi AKPOLAT ◽  
Özkan GÖRGÜLÜ ◽  
İbrahim KAPLAN
Keyword(s):  
Electromagnetic Field ◽  
Magnetic Fields ◽  
Whole Blood ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Blood Parameters ◽  
Low Density ◽  
Distribution Width ◽  
Significant Difference ◽  
Experimental Group

The electromagnetic field is increasing in our environment and these exposures cause concern. The aim of our study is to investigate the effects of magnetic fields generated by the hairdryer devices used by women working in the same job on their serum biochemistry, whole blood, and hormone values. The sixteen women working continuously in hairdressing salons were included in the study. Two groups of studies were designed: control (n:8, mean age: 22.25±6.04) and experimental group (n:8, mean age:23,62±6.67). The biochemical (median values of alanine aminotransferase, aspartate aminotransferase, triglycerides, and very-low-density lipoprotein were found high) and hormonal results of the experimental group were compared with the biochemical (cholesterol, low-density lipoprotein, and very high-density lipoprotein had high median values) and hormonal results of the control group and no significant difference was found (p>0.05). When the whole blood parameters were examined, the white blood cells and mean platelet volume results of the experimental group were significant (p<0.05), while there was a meaningless difference between red blood cell, hemoglobin, hematocrit, mean corpuscular volume, red cell distribution width and platelet values (p>0.05). It is evident from the results that occupational exposure to magnetic fields constantly leads to changes in the biochemistry, hormone and whole blood parameters of the female.

Abstract 302: Presence of Oxidized Low-Density Lipoprotein in the Left Ventricular Blood of Subjects with Cardiovascular Diseases

2012 ◽  
Vol 32 (suppl_1) ◽  
Author(s):  
Chandrakala Aluganti Narasimhulu ◽  
Dmitry Litvinov ◽  
Danielle Jones ◽  
Chittoor Sai-Sudhakar ◽  
Michael Fristenberg ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Left Ventricular ◽  
Paraoxonase 1 ◽  
Left Ventricular Ejection ◽  
Low Density ◽  
Oxidized Low Density Lipoprotein ◽  
Peripheral Tissues ◽  
Significant Difference

Hypothesis: Oxidized low density lipoprotein (Ox-LDL) has properties that profoundly affect cardiovascular function. We hypothesized that Ox-LDL is likely to be formed in the left ventricular blood (LVB) when the heart is subjected to ischemic conditions and the ejection fraction (EF) is low. We speculated whether “stagnation” of LDL in the LV could result in increased formation of Ox-LDL. Objective: We studied whether there is an increased level of Ox-LDL in the LVB as opposed to peripheral blood (PB), and whether its presence correlated with the EF. Also we examined whether a higher level of Ox-LDL negatively correlated with the activity of paraoxonase 1 (PON 1). Methods: Following the Institutional Review Board (IRB) approval, 62 HF patients were enrolled in the study. All patients underwent pre-operative transthoracic echocardiographic assessment of ventricular function. Left ventricular ejection fractions were determined using the Simpsons bi-plane technique. 2ml of LVB and 5ml of PB samples were taken before coronary artery bypass surgery, or a surgery with replacement of mitral, aortic or tricuspid valve. Blood level of Ox-LDL was determined by ELISA (Mercodia), and PON 1 activity was determined by the rate of conversion of its substrate p-nitrophenyl acetate into p-nitrophenol. Results: The result showed significant increase in Ox-LDL in LVB as compared to PB (p=0.032) in HF subjects even when EF was near normal. There was no significant increase in subjects with lower EF. In contrast, Ox-LDL levels increased in the PB of subjects with lower EF and reached those of LVB. We also noticed that there was a statistically significant negative correlation between EF and Ox-LDL levels in both LVB and PB (p < 0.05). The activity of PON1, an antioxidant enzyme that protects LDL from oxidation showed decreased levels both in LV blood as well as in PB with decreased EF. It was observed that there was a statistically significant difference in PON1 levels between LV and PB of subjects having EF>60% (p = 0.03). Conclusions: In conclusion the results suggest that there might be oxidative stress associated with LVB even when the EF is not compromised. In contrast, the increase in PB Ox-LDL with poor EF might suggest that the low blood flow to peripheral tissues and end organs also might contribute to increased oxidative stress. The results also might suggest that persistent oxidative stress could have affected the clearance mechanisms of Ox-LDL.

Estimation of Low-Density Lipoprotein Immunoprecipitates in Uniform Drops of Serum

1962 ◽  
Vol 8 (6) ◽  
pp. 616-625 ◽  
Author(s):  
R L Searcy ◽  
T M Asher ◽  
L M Bergquist
Keyword(s):  
Diabetes Mellitus ◽  
Coronary Artery Disease ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Healthy Population ◽  
Low Density ◽  
Significant Difference ◽  
Microcapillary Columns ◽  
Artery Disease ◽  
Ultracentrifugal Analysis

Abstract An immunochemical technic has been found to be specific, efficient and reproducible in removing lipoproteins of density less than 1.063 gm./ml. from measured drops of human serum. Estimations of microcapillary columns of immunoprecipitate compare favorably with ultracentrifugal analysis of Sf 0-400 lipoproteins. An equation derived from these data converts millimeters of lipoprotein precipitate into milligrams lipoprotein Sf 0-400 per 100 ml. serum. A highly significant difference was obtained between mean lipoprotein precipitate levels of a clinically healthy population and those of a group of patients with coronary artery disease or diabetes mellitus.

scholarly journals Lectin-like, oxidized low-density lipoprotein receptor-1-deficient mice show resistance to age-related knee osteoarthritis

2017 ◽  
Author(s):  
Kazuhiko Hashimoto ◽  
Yutaka Oda ◽  
Fumihisa Nakamura ◽  
Ryosuke Kakinoki ◽  
Masao Akagi
Keyword(s):  
Articular Chondrocytes ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Oxidized Low Density Lipoprotein ◽  
Expression Levels ◽  
Knee Oa ◽  
Age Related ◽  
Significant Difference ◽  
The Mean

The lectin-like, oxidized low-density lipoprotein (ox-LDL) receptor-1 (LOX-1)/ox-LDL system contributes to atherosclerosis and may be involved in cartilage degeneration. The purpose of this study was to determine whether the LOX-1/ox-LDL system contributes to age-related osteoarthritis (OA) in vivo, using LOX-1 knockout (LOX-1 KO) mice. Knee cartilage from 6, 12, and 18-month old (n = 10/group) C57Bl/6 wild-type (WT) and LOX-1 KO mice was evaluated by determining the Osteoarthritis Research Society International (OARSI) score of Safranin-O stained samples. The prevalence of knee OA in both mouse strains was also investigated. Expression levels of LOX-1, ox-LDL, runt-related transcription factor-2 (Runx2), type-X collagen (COL X), and matrix metalloproteinase-13 (MMP-13) in the articular chondrocytes were analyzed immunohistologically. No significant difference was observed in the mean scores of WT (2.00±0.61) and LOX-1 KO mice (2.00±0.49) at 6 months of age (P=1.00, n=10). At 12 and 18 months of age, the mean scores of LOX-1 KO mice (3.75±0.93 and 5.50±0.78) were significantly lower than those of WT mice (5.25±1.14 and 9.00±1.01; P<0.001 in both cases; n=10). The prevalence of OA in LOX-1 KO mice was lower than that in WT mice at 12 and 18 months of age (40 vs 70%, 70 vs 90%, respectively; n=10). The expression levels of Runx2, COL X, and MMP-13 in articular chondrocytes significantly decreased in LOX-1 KO, mice compared with those in WT mice. The study indicated that the LOX-1/ox-LDL system in chondrocytes plays a role in the pathogenesis of age-related knee OA, which is potentially a target for preventing OA progression.

scholarly journals Metabolic evaluation in overweight and obese cats and association with blood pressure

2017 ◽  
Vol 48 (1) ◽  
Author(s):  
Vanessa Danielle de Freitas ◽  
Alessandra Ramos Castilho ◽  
Luciana Auxiliadora Viebrantz da Conceição ◽  
Valéria Régia Franco Sousa ◽  
Adriane Jorge Mendonça ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Serum Levels ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Metabolic Evaluation ◽  
Morphometric Measurements ◽  
Metabolic Alterations ◽  
Significant Difference

ABSTRACT: Obesity has been increasing in cats andis associated with metabolic and cardiovascular diseases. The association of these alterations can trigger the onset of metabolic syndrome (MS). Therefore, this study aimed to analyze the serum levels of glucose, fructosamine, cholesterol, triglycerides, high-density lipoprotein, low-density lipoprotein, very low-density lipoprotein (VLDL), andalanine aminotransferase of cats and to identify the possibility of MS,as well as to evaluate changes in arterial pressure. Thirty-seven cats were classified by ECC and morphometric measurements, namely, 15 obese, 12 overweight, and 10 controls. Nocat manifested MS. Only VLDL had a statistically significant difference (P<0.05) between groups. Therefore, obesity may not be associated with arterial hypertension, and more studies are needed to evaluate the metabolic alterations in overweight and obese cats.

Evidence for separate pathways of chylomicron and very low-density lipoprotein assembly and transport by rat small intestine

1984 ◽  
Vol 247 (6) ◽  
pp. G599-G610 ◽  
Author(s):  
P. Tso ◽  
D. S. Drake ◽  
D. D. Black ◽  
S. M. Sabesin
Keyword(s):  
Small Intestine ◽  
Lipid Emulsion ◽  
Low Density Lipoprotein ◽  
Sodium Taurocholate ◽  
Density Lipoprotein ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Intestinal Lymph ◽  
Significant Difference ◽  
Egg Lecithin

Previously, we demonstrated that the hydrophobic surfactant Pluronic L-81 (L-81) inhibits the intestinal formation and transport of chylomicrons (CM) but not of very low-density lipoprotein-sized (VLDL) particles. The present study was undertaken to determine whether infusion of egg lecithin results mainly in secretion of VLDL by the small intestine and whether L-81 has any effect on their formation and secretion. Intestinal fistula rats were infused intraduodenally at a rate of 3 ml/h with a lipid emulsion containing 20 mM egg lecithin and 19 mM sodium taurocholate for 8 h. This was then followed by another 8 h of infusion of a similar lipid emulsion but with 0.5 mg/h of L-81 added. Lymphatic lipid output was measured, and lymph lipoproteins were sized by use of electron microscopy. Whether L-81 was present or not, no significant difference was detected in the lymphatic triglyceride, phospholipid, or cholesterol outputs. Based on agarose gel electrophoresis, sizing of intestinal lymph lipoproteins, and also the determination of lipid in the intestinal lymph CM and VLDL as separated by ultracentrifugation, VLDL were the major lipoproteins present in lymph during the infusion of egg lecithin. Thus, intraduodenal infusion of egg lecithin in the rat results mainly in the transport of VLDL and is not affected by the administration of L-81. The results suggest that CM and VLDL are assembled separately by the enterocytes and indicate the usefulness of L-81 in further investigating the pathways and regulation of intestinal lipoprotein synthesis, assembly, and secretion.

scholarly journals Effect of Hyper and Hypothyroidism on Lipid Profile and Liver Function of Male Rats

2011 ◽  
Vol 8 (4) ◽  
pp. 926-933
Author(s):  
Baghdad Science Journal
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Physiological Saline ◽  
Serum Levels ◽  
Lipoprotein Cholesterol ◽  
Male Rats ◽  
Control Group ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Significant Difference

This study was designed to investigate the effect of thyroid hormone disturbance on lipids profiles and liver functions. Eighteen mature male rats Rattus norvegicus were divided into three groups. The first and the second groups were injected subcutaneously with thyroxine (T4) and carbimazol (both at 600 µg/kg BW) respectively on alternate days, to produce recurrent periods of hyper and hypothyroidism .The control group which is the third group was injected with physiological saline. The process continued 4 weeks, after that, injection, blood specimens were collected to estimate serum levels of T3 and T4, Total cholesterol (TC), Triglycerides (TG), High density lipoprotein cholesterol (HDL-C), Low density lipoprotein cholesterol (LDL-C) and Very low density lipoprotein cholesterol (VLDL-C) were determined in the serum. In addition to that, we measure GOT, GPT and CPK enzymes activity. The results showed no significant difference in body weight and a significant increase (P

scholarly journals ASSOCIATION OF SUBCLINICAL HYPOTHYROIDISM IN METABOLIC SYNDROME PATIENTSASSOCIATION OF SUBCLINICAL HYPOTHYROIDISM IN METABOLIC SYNDROME PATIENTS

2018 ◽  
Vol 11 (9) ◽  
pp. 188
Author(s):  
Jennifer S Suhashini ◽  
Savitha G
Keyword(s):  
Metabolic Syndrome ◽  
Subclinical Hypothyroidism ◽  
Low Density Lipoprotein ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Significant Difference ◽  
Tsh Levels

Objectives: Subclinical hypothyroidism (SCH) is also the major risk for cardiovascular disease like metabolic syndrome (MetS). Hence, the aim of this study is to assess the association of SCH in MetS patients.Materials and Methods: Ninety patients reporting to Saveetha Dental College and Hospitals were enrolled in the study which includes 40 patients with MetS and 40 healthy individuals. 5 ml of venous blood was collected and centrifuged. Then, it is analyzed for fasting blood sugar, serum triglycerides, high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), and very low-density lipoprotein (VLDL) using the standard kit method. Then, Free T3, Free T4, and thyroid-stimulating hormone (TSH) were estimated by ELISA method. The data obtained were subjected to statistical analysis using the SPSS software.Results: SCH is 20% in cases when compared to 4.4% in controls, which was significant, p=0.024. The biochemical parameters were compared between the study population fasting blood glucose, triglycerides, total cholesterol, low-density lipoprotein cholesterol, and VLDL cholesterol was statistically significant, with p<0.001. TSH levels showed significant difference between two groups with the p=0.002.Conclusion: MetS patients should be screened for the SCH as an important risk factor in evaluation protocol. Mere correction of TSH levels can reverse the associated morbidity in these patients rather than leaving them untreated pushing them to a state of overt hypothyroidism with its attendant complications.

Transport of Beta-Carotene in Serum of Individuals with Carotenemia

1974 ◽  
Vol 20 (12) ◽  
pp. 1578-1579 ◽  
Author(s):  
Micheline M Mathews-Roth ◽  
Christian L Gulbrandsen
Keyword(s):  
High Density Lipoprotein ◽  
Serum Lipid ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Beta Carotene ◽  
Relative Increase ◽  
High Density ◽  
Low Density ◽  
Lipid Fractions ◽  
Significant Difference

Abstract We studied distribution of carotene in the various serum lipid fractions of carotenemic and noncarotenemic individuals. We found that the amount of carotene in each lipoprotein fraction is increased in the serum from carotenemic individuals, but that the relative increase is significant only in the low-density lipoprotein and high-density lipoprotein fractions. There was no significant difference between the cholesterol concentrations of each fraction of the carotenemic individuals' and noncarotenemic individuals' sera, indicating that serum betalipoprotein is not increased in carotenemia.

scholarly journals Alterations of Lipid profile levels in 7,12-Dimethylbenz(a)anthracene induced ulcerative colitis rat model.

2017 ◽  
Vol 10 (4) ◽  
pp. 352
Author(s):  
Geetha Palaniswamy ◽  
Lakshman Kumar B ◽  
Indra U ◽  
Pavithra Sheetal B
Keyword(s):  
Ulcerative Colitis ◽  
High Density Lipoprotein ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Intestinal Inflammation ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Density ◽  
Low Density ◽  
Significant Difference

Objective: Ulcerative colitis is a type of inflammatory bowel disease is a chronic gastrointestinal disorder characterized by intestinal inflammation and mucosal tissue damage. We examined the lipid profile levels in murine model of 7,12 Dimethylbenz(a)anthracene induced ulcerative colitis.Methods: Serum was separated from whole blood and was used to determine the lipid profile such as total cholesterol (TC), phospholipids (PL), triglycerides (TG), free fatty acids, high density lipoprotein (HDL-C) and low density lipoprotein (HDL-C).Results: Ulcerative colitis rats exhibit low level of low density lipoprotein cholesterol and total cholesterol. No significant difference was observed in high density lipoprotein and triglycerides and significant difference was observed in phospholipids and free fatty acid serum levels. This communication highlights the lipid profile that occurs in ulcerative colitis.Conclusion: This study, thus, provides valuable information about the disturbances in the lipids and lipoproteins occur in ulcerative colitis.Keywords: Ulcerative colitis, 7,12-Dimethylbenz(a)anthracene, Lipoprotein, Low-density lipoprotein, Phospholipids.

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