Water Extract Activity of Papaya Leaf as Antibiofilm against Escherichia coli

2017 ◽  
Vol 2 (3) ◽  
pp. 164-175
Author(s):  
Livia Rhea Alvita ◽  
Syamsul Falah ◽  
Novik Nurhidayat
Keyword(s):  
Escherichia Coli ◽  
Shelf Life ◽  
Aqueous Extract ◽  
Contact Time ◽  
Food Industry ◽  
Cell Attachment ◽  
Water Extract ◽  
Optimum Temperature ◽  
Food Spoilage ◽  
Extract Concentration

Biofilms are significant hazards in the food industry. The presence of Escherichia coli biofilms in food industry is potentially causing food spoilage that will shorten the shelf life as well as lead to the spread of disease through food. The purpose of this study is to determine the phytochemical compound of aqueous extract of papaya leaves and to determine its activity on inhibition of cell attachment and growth, and on degradation of the biofilm using Micro-titter Plate Biofilm Assay. The results phytochemical test showed that papaya leave extract contains alkaloid, tanin, flavonoid, and steroid and have anti-biofilm activity. Papaya leave extract with highest activity caused biofilm degradation of 48,99%. The optimum temperature of biofilm degradation (>52,5%) by papaya leaves extract was around 35oC - 40oC. The optimum contact time and extract concentration were 57-60 minutes and 75-100%, respectively.

The In Vitro Antibiofilm Activity of Water Leaf Extract of Papaya (Carica papaya L.) against Pseudomonas aeruginosa

2017 ◽  
Vol 2 (3) ◽  
pp. 150-163
Author(s):  
Ekajayanti Kining ◽  
Syamsul Falah ◽  
Novik Nurhidayat
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Contact Time ◽  
Cell Attachment ◽  
Water Extract ◽  
Opportunistic Pathogen ◽  
Bacterial Biofilm ◽  
Antibiofilm Activity ◽  
Bacterial Survival ◽  
Optimum Conditions

Pseudomonas aeruginosa is one of opportunistic pathogen forming bacterial biofilm. The biofilm sustains the bacterial survival and infections. This study aimed to assess the activity of water extract of papaya leaves on inhibition of cells attachment, growth and degradation of the biofilm using crystal violet (CV) biofilm assay. Research results showed that water extract of papaya leaves contains alkaloids, tanins, flavonoids, and steroids/terpenoids and showed antibacterial activity and antibiofilm against P. aeruginosa. Addition of extract can inhibit the cell attachment and was able to degrade the biofilm of 40.92% and 48.058% respectively at optimum conditions: extract concentration of 25% (v/v), temperature 37.5 °C and contact time 45 minutes. With a concentration of 25% (v/v), temperature of 50 °C and the contact time of 3 days, extract of papaya leaves can inhibit the growth of biofilms of 39.837% v/v.

scholarly journals Efecto del extracto acuoso de la Ocimum basilicnum l. (albahaca) en el crecimiento bacteriano de Escherichia coli

2018 ◽  
pp. 132-140
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Bacterial Growth ◽  
Antibacterial Effect ◽  
Water Extract ◽  
Ocimum Basilicum ◽  
Control Group ◽  
Popular Knowledge ◽  
Escherichia Coli Bacteria

Efecto del extracto acuoso de la Ocimum basilicnum l. (albahaca) en el crecimiento bacteriano de Escherichia coli Effect of aqueous extract of Ocimum basilicnum l. (basil) on bacterial growth of Escherichia coli Jherson Calderon Mestanza1, Elías A. Torres Armas2 1 CONSECUENCIA. 2 Universidad Nacional Toribio Rodríguez de Mendoza. DOI: https://doi.org/10.33017/RevECIPeru2013.0018/ Resumen El presente estudio es de diseño de investigación experimental, completamente aleatorizado con 4 tratamientos y 1 grupo testigo, con 3 repeticiones. Cuyo objetivo general fue determinar el efecto antibacteriano del extracto acuoso de albahaca (Ocimum basilicum l.) en el crecimiento bacteriano de Escherichia coli, a fin de dar el sustento científico al conocimiento popular. Se trabajó en una muestra constituida por 15 placas petri con cepas de Escherichia coli y material vegetal hojas de albahaca (Ocimum basilicum l.) en fresco. Los resultados muestran un efecto positivo de la actividad antibacteriana para las dosis de 5g, 10g y 20g del extracto acuoso de albahaca (Ocimum basilicum l.) en el crecimiento bacteriano de Escherichia coli, cuyo efecto antibacteriano para la dosis de 5g de extracto acuoso de albahaca (Ocimum basilicum l.) fue de 38 %, para 10g fue de 43.5 %, y para 20g fue de 46.5 %. En conclusión, el crecimiento bacteriano de Escherichia coli disminuye significativamente por efecto de las tres dosis (5g, 10g y 20g) de extracto acuoso de albahaca (Ocimum basilicum l.) procedente de la comunidad de Taquia de la Región Amazonas; siendo la dosis de 20g la que tuvo mayor actividad antibacteriana. Descriptores: Extracto acuoso de Ocimum basilicum l., actividad antibacteriana en Escherichia coli. Abstract This study is of research design experiment, completely randomized with 4 treatments and 1 control group, with 3 repetitions. Whose overall objective was to determine the antibacterial effect of aqueous extract of basil (Ocimum basilicum l.) in bacterial growth of Escherichia coli, in order to provide scientific support to popular knowledge. Worked on a sample of 15 petri dishes with strains of Escherichia coli and plant material basil (Ocimum basilicum l.) Fresh. The results show a positive effect of antibacterial activity at doses of 5g, 10g, and 20g water extract of basil (Ocimum basilicum l.) On the growth of Escherichia coli bacteria whose antibacterial effect dose aqueous extract 5g basil (Ocimum basilicum l.) was 38% for 10g was 43.5%, and 46.5% was 20g. In conclusion bacterial growth of Escherichia coli significantly reduces the effect of three doses (5g, 10g and 20g) of aqueous extract of basil (Ocimum basilicum l.) community from Turning Lady of the Amazon Region, being the 20mg which had higher antibacterial activity. Keywords: Aqueous extract of Ocimum basilicum l., antibacterial activity in Escherichia coli.

Research on Antibacterial Activity of Crude Extract from Mangifera Indica Leaves

2013 ◽  
Vol 781-784 ◽  
pp. 760-763
Author(s):  
Shao Juan Jiang ◽  
Ying Zhang
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Aqueous Extract ◽  
Mangifera Indica ◽  
Petroleum Ether Extract ◽  
Water Extract ◽  
Inhibitory Effect ◽  
Saccaromyces Cerevisiae

The 95% ethanol extracts of mangifera indica leaf was sequentially extracted with petroleum ether, ethyl acetate, n-butyl alcohol and water. Antibacterial activity and minimal inhibitory concentration (MIC) of the four different extraction against Staphylococlus aureus, Escherichia coli, Bacillus subitlis and Saccaromyces cerevisiae were stuied with filter paper method in this paper. Each of the four extracts showed different antibacterial activity: The water extract is the strongest one; l-butanol extract takes second place; Ethyl acetate and petroleum ether extract is the third. MIC of aqueous extract with the strongest inhibitory effect on Staphylococlus aureus, Escherichia coli and Saccaromyces cerevisiae is 0.0125 g/mL, 0.0125 g/mL, and 0.025 g/mL. Moreover, the aqueous extract showed powerful antibacterial activity against all of four bacteria.

Evaluation of Fruit Extracts Influence on the Susceptibility of Escherichia Coli Rods to the Bactericidal Action of Human Serum

2021 ◽  
Author(s):  
Agnieszka Cisowska ◽  
Janina Gabrielska
Keyword(s):  
Escherichia Coli ◽  
Human Serum ◽  
Mechanisms Of Action ◽  
Bactericidal Action ◽  
E Coli ◽  
Fruit Extracts ◽  
Extract Concentration ◽  
Japanese Quince ◽  
Normal Human ◽  
Dog Rose

Abstract This study determined the influence of the methanol (ME) and water (WE) fruit extracts obtained from eight species of Rosaceae and Grossulariacae family on the susceptibility of Escherichia coli rods to the lytic action of normal human serum (NHS). Bacteria were incubated for 24 h in tryptic soy broth with varying concentrations (1, 5, 10, 20, 30, 40, and 50 mg ml-1) of raspberry, cherry, hawthorn, dog rose, gooseberry, chokeberry, quince, and Japanese quince extracts and then the bactericidal activity of NHS was established. We found that the resistance of E. coli rods to the bactericidal action of serum was altered by prior incubation with all tested extracts and was dependent on plant extract concentration. Among the tested extracts, gooseberry (both ME and WE), raspberry ME and cherry WE were responsible for the most profound changes in serum resistance of E. coli rods. Evaluation of the antimicrobial mechanisms of action of phenolics-rich plant extracts has the potential to impact the development of novel compounds with promising applications in food and biopharmaceutical industry or medical approaches to preventing and treating pathogenic infections.

Irradiation D-Values for Escherichia coli O157:H7 and Salmonella sp. on Inoculated Broccoli Seeds and Effects of Irradiation on Broccoli Sprout Keeping Quality and Seed Viability†

2003 ◽  
Vol 66 (5) ◽  
pp. 760-766 ◽  
Author(s):  
KATHLEEN T. RAJKOWSKI ◽  
GLEN BOYD ◽  
DONALD W. THAYER
Keyword(s):  
Escherichia Coli ◽  
Shelf Life ◽  
Dose Level ◽  
Germination Percentage ◽  
Yield Ratio ◽  
Escherichia Coli O157 ◽  
Broccoli Sprouts ◽  
Sprout Length ◽  
D Values ◽  
Irradiation Process

Like alfalfa sprouts, broccoli sprouts can be a vehicle for bacterial pathogens, which can cause illness when they are consumed. The gamma irradiation process was used to reduce numbers of bacterial pathogens on broccoli sprouts and seeds, and the effect of this process on the seeds was studied. The irradiation destruct values for Salmonella sp. and for strains of Escherichia coli O157:H7 inoculated on broccoli seeds were determined. Results obtained in this study indicate that a dose of 2 kGy reduced total background counts for broccoli sprouts from 106 to 107 CFU/g to 104 to 105 CFU/g and increased the shelf life of the sprouts by 10 days. Yield ratio (wt/wt), germination percentage, sprout length, and thickness were measured to determine the effects of various irradiation doses on the broccoli seeds. Results show a decreased germination percentage at a dose level of 4 kGy, whereas the yield ratio (wt/wt), sprout length, and thickness decreased at the 2-kGy dose level. The radiation doses required to inactivate Salmonella sp. and strains of E. coli O157:H7 were higher than previously reported values. D-values, dose required for a 1-log reduction, for the nonvegetable and vegetable Salmonella sp. isolates were 0.74 and 1.10 kGy, respectively. The values for the nonvegetable and vegetable isolated strains of Escherichia coli O157:H7 were 1.43 and 1.11 kGy, respectively. With the irradiation process, a dose of up to 2 kGy can extend the shelf life of broccoli sprouts. A dose of >2 kGy would have an adverse effect on the broccoli seed and decrease the yield of broccoli sprouts.

scholarly journals Preparation of Sticky Escherichia coli through Surface Display of an Adhesive Catecholamine Moiety

2013 ◽  
Vol 80 (1) ◽  
pp. 43-53 ◽  
Author(s):  
Joseph P. Park ◽  
Min-Jung Choi ◽  
Se Hun Kim ◽  
Seung Hwan Lee ◽  
Haeshin Lee
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Communication Systems ◽  
Cell Attachment ◽  
Surface Display ◽  
Content Type ◽  
E Coli ◽  
Material Surfaces ◽  
Mussel Adhesive ◽  
Adhesive Proteins

ABSTRACTMussels attach to virtually all types of inorganic and organic surfaces in aqueous environments, and catecholamines composed of 3,4-dihydroxy-l-phenylalanine (DOPA), lysine, and histidine in mussel adhesive proteins play a key role in the robust adhesion. DOPA is an unusual catecholic amino acid, and its side chain is called catechol. In this study, we displayed the adhesive moiety of DOPA-histidine onEscherichia colisurfaces using outer membrane protein W as an anchoring motif for the first time. Localization of catecholamines on the cell surface was confirmed by Western blot and immunofluorescence microscopy. Furthermore, cell-to-cell cohesion (i.e., cellular aggregation) induced by the displayed catecholamine and synthesis of gold nanoparticles on the cell surface support functional display of adhesive catecholamines. The engineeredE. coliexhibited significant adhesion onto various material surfaces, including silica and glass microparticles, gold, titanium, silicon, poly(ethylene terephthalate), poly(urethane), and poly(dimethylsiloxane). The uniqueness of this approach utilizing the engineered stickyE. coliis that no chemistry for cell attachment are necessary, and the ability of spontaneousE. coliattachment allows one to immobilize the cells on challenging material surfaces such as synthetic polymers. Therefore, we envision that mussel-inspired catecholamine yielded stickyE. colithat can be used as a new type of engineered microbe for various emerging fields, such as whole living cell attachment on versatile material surfaces, cell-to-cell communication systems, and many others.

scholarly journals Usages of Salvia officinalis Boiled Water Extract for Pathogenic Escherichia coli Exclusion at Elevated Region

2021 ◽  
Vol 2 (3) ◽  
pp. 01-03
Author(s):  
Sherifa Sabra
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Water Extract ◽  
Salvia Officinalis ◽  
Bacterial Suspension ◽  
Water Extracts ◽  
Pathogenic Escherichia Coli ◽  
Chemical Content ◽  
Boiled Water ◽  
Chemical Contents

The aim was for tryout using daily regular Salvia officinalis boiled water extracts in "Arab and Saudi Society", especially at elevated region "Taif". That to prove its ability for pathogenic Escherichia coli exclusion and had antibiotic resistance. This could prove strength of chemical content and adherence of "Arab People" to use as regular and daily to stabilize body health and protect against pathogenic bacteria. Salvia officinalis was purchased from pharmacy at "Taif" and boiled water extract was made. Pathogenic Escherichia coli was obtained from a "Licensed Health Center"; culture was made and suspensions. Tryout by boiled water extract were added to bacterial suspension and then were kept in incubator. The samples were taken at hours (1, 3, 5, 7, 9, 11, 13, 15, 17 and 19); were cultured and were kept for 48 hours at 37 °C. The exclusion percent's had determined, Escherichia coli exclusion mean percentages rose 5% at third hour and then increased 7% at fifth hour and at ninth hour was 8%. Then it decreased at the eleventh hour and thirteenth hour (2% and 3%), then rose at the fifteenth hour 10%. It decreased 5% at the seventeenth hour, and then returned and rose at the nineteenth hour 12%. It was concluded Salvia officinalis boiled water extracts effects on pathogenic Escherichia coli exclusion was found during one growth bacteria cycle, indicated the characteristics of chemical contents and good daily habit. It was recommend continuing as "Arabian Habit" Salvia officinalis boiled water extracts for health and protection stability.

scholarly journals Evaluation du potentiel antimicrobien et de la toxicité des extraits de Jatropha multifida Linn, (Euphorbiaceae)

2020 ◽  
Vol 151 ◽  
pp. 15550-15558
Author(s):  
Amégninou Agban ◽  
Yao Hoekou ◽  
Passimna Pissang ◽  
Tchadjobo Tchacondo ◽  
Komlan Batawila
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Aqueous Extract ◽  
E Coli ◽  
Toxicological Studies ◽  
Jatropha Multifida

Objectif : L’objectif de ce travail était d’évaluer in vitro l’activité antimicrobienne des extraits de feuilles et tige de Jatropha multifida sur la croissance de Candida albicans, Escherichia coli et Staphylococcus aureus, puis d’évaluer in vivo la toxicité de cette plante. Méthodologie et résultats : Les méthodes de diffusion en milieu gélosé et de microdilution en milieu liquide ont été utilisées pour évaluer l’effet antimicrobien. Une étude en subaigüe était réalisée afin d’explorer les effets toxiques de l’extrait aqueux des feuilles. Les résultats des tests antimicrobiens montrent une activité des extraits de feuilles et tige de J. multifida sur la croissance des souches utilisées avec des diamètres de zones d’inhibition allant de 8 à 25 mm et des concentrations minimales inhibitrices (CMI) variant de 0,039 mg/mL à 1,25 mg/mL à l’exception des souches de E. coli qui sont résistantes aux extraits de la tige. L’administration en subaigüe de l’extrait aqueux des feuilles de J. multifida à la dose de 600 mg/kg entraîne une perte significative de poids chez les souris. Conclusion et applications des résultats : Les extraits aqueux, éthanolique et hydroéthanolique des feuilles et tige de J. multifida possèdent d’activité antimicrobienne et pourraient être utilisés dans le traitement des Candidoses à C. albicans et des infections à S. aureus. Mais l’essai de toxicité subaigüe montre que l’extrait aqueux de la plante serait toxique. Des études toxicologiques approfondies restent donc nécessaires sur ces extraits afin de mieux élucider leur inocuité. Mots-clés : Jatropha multifida, extraits de feuilles et de tige, activités antifongique et antibactérienne, toxicité. Agban et al., J. Appl. Biosci. 2020 Evaluation du potentiel antimicrobien et de la toxicité des extraits de Jatropha multifida Linn, (Euphorbiaceae) 15551 Evaluation of antimicrobial potential and toxicity of Jatropha multifida Linn, (Euphorbiaceae) extracts ABSTRACT Objective: The objective of this study was to evaluate in vitro the antimicrobial activity of leaves and stem of Jatropha multifida extracts against Candida albicans, Escherichia coli and Staphylococcus aureus, and then to evaluate in vivo the toxicity of this plant. Methodology and Results: The agar well-diffusion and the NCCLS broth microdilution methods were used to assess the antimicrobial effect. A subacute study was carried out to explore the toxic effects of the aqueous extract of the leaves. The results of the antimicrobial tests show an activity of the extracts of leaves and stems of J. multifida on the growth of the strains used with diameters of inhibitory zones ranging from 8 to 25 mm and minimum inhibitory concentrations (MIC) varying from 0.039 mg/mL to 1.25 mg/mL exception E. coli strains which are resistant to extracts from the stem. Subacute administration of the aqueous extract of the leaves of J. multifida at a dose of 600 mg/kg leads to a significant loss of weight in the mice. Conclusion and application of findings : The aqueous, ethanolic and hydroethanolic extracts of the leaves and stem of J. multifida have antimicrobial activity and could be used in the treatment of Candidiasis and bacterial infections due respectively to C. albicans and S. aureus. But the subacute toxicity test shows that the aqueous extract of the plant would be toxic. Extensive toxicological studies therefore remain necessary on these extracts in order to better elucidate their safety. Keywords: Jatropha multifida extracts of leaves and stem, antifungal and antibacterial activities, toxicity

scholarly journals The anthocyanin pigment extract from red rose as antibacterial agent

2018 ◽  
Vol 14 (1-2) ◽  
pp. 184-187
Author(s):  
Elfi Anis Saati ◽  
Annisa Dyah Pusparini ◽  
Mochammad Wachid ◽  
Sri Winarsih
Keyword(s):  
Escherichia Coli ◽  
Antioxidant Activity ◽  
Solvent Extraction ◽  
Shelf Life ◽  
Antibacterial Agent ◽  
Block Design ◽  
Pseudomonas Sp ◽  
Anthocyanin Pigment ◽  
Extraction Solvent ◽  
Pigment Extract

Red rose consisting of anthocyanin pigment has been used as an antibacterial agent. However, there is no study on the anthocyanin pigment extract from red rose as the antibacterial agent. The effectiveness of the antibacterial agent can be affected by the solvent extraction and the flower shelf life. Here, we report the effects of solvent extraction and red rose (Rosa sp.) shelf life on the antibacterial activity. Red rose concentrated extraction and randomized complete block design factorial was carefully used with factors of long display and solvent extraction. The extraction solvent (P) comprised of water, ethanol, and mixture of water-ethanol (1 : 1), while the red rose shelf life (M) consisted of 0, 2, 4 and 6 days. Moreover, pH, antioxidant activity and minimum bactericidal concentration (MBC) with four variations of concentrated concentration (100%, 50%, 25% and 12.5%) on Escherichia coli, Salmonella thypi, and Pseudomonas sp. were analyzed. After two days of shelf life using water as the solvent for extraction, the antioxidant activity achieved 79% at pH of 2.5. It was also demonstrated that it was able to kill all the investigated bacterias, which were Escherichia coli, Salmonella thypi, and Pseudomonas sp. with concentrated concentrations of 100%, 50% and 25%. When the concentrated concentration was 12.5%, the MBC value was constantly found to be 1.39 × 108 cfu/g for Escherichia coli and 9.53 × 107 cfu/g for Salmonella thypi.

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