scholarly journals Perlakuan Benih Cabai (Capsicum annuum L.) dengan Rizobakteri secara Tunggal atau Kombinasi dapat Mengendalikan Phytophthora capsici dan Meningkatkan Pertumbuhan Tanaman

2015 ◽  
Vol 6 (1) ◽  
pp. 1 ◽  
Author(s):  
Farih Najah Rosadiah ◽  
Satriyas Ilyas ◽  
Dyah Manohara
Keyword(s):  
Seed Treatment ◽  
Phytophthora Capsici ◽  
Positive Control ◽  
Negative Control ◽  
Hot Pepper ◽  
Phytophthora Blight ◽  
Randomized Design ◽  
Phytophthora Rot ◽  
Blight Disease

<p>ABSTRACT</p><p>Seed  treatment  of  hot  pepper  using  rhizobacteria  is  an  alternative  to fungicide  use  in controlling  phytophthora  rot  disease.  The  objectives  of this  research  were  to  evaluate: (1)  the effectiveness of rhizobacteria isolates in inhibiting Phytophthora capsici growth and (2) the effect of seed treatment using rhizobacteria on  plant growth,  and  incidence of phytophthora blight  disease. This  research  consisted  of  two  experiments,  all experiments were  arranged in  completely randomized design using one factor. The first experiment (in vitro) consisted of nine levels i.e. sevencombination isolates of rhizobacteria, metalaxyl and control. The second experiment (in the green house)  consisted  of  six  levels  of  seed  treatments  i.e.  ST116B rhizobacteria,  CM8  rhizobacteria, ST116B + CM8  rhizobacteria, metalaxyl, positive control  and negative control. Results of in vitro experiment  showed that  all  rhizobacteria,  single  or  combinations,  were  able  to  inhibit  P. capsicigrowth. The  highest  inhibition  were  shown  by  CM8,  ST116B  + CM8, and ST116B  consecutively.Seed treatments of hot pepper using ST116B, CM8, and ST116B + CM8 rhizobacteria increased the number  of  leaves  6  weeks after  transplanting  and  reduced  the  incidence  of  phytophthora blight disease.  There were no significant  differences whether  the rhizobacteria was applied  singly  or  in combination  of  the  two.  Rhizobacteria  ST116B was  suggested  for  pepper  seed  treatment  before planting.</p><p>Keywords: metalaxyl, phytophthora blight disease, rhizobacteria</p><p> </p><p>ABSTRAK</p><p>Perlakuan  benih  cabai  menggunakan  rizobakteri  merupakan  alternatif pengganti  fungisida dalam  mengendalikan  penyakit  busuk  phytophthora, yang  disebabkan  oleh  cendawan  patogen Phytophthora  capsici.  Penelitian ini bertujuan (1) mengevaluasi keefektifan  kombinasi  isolat rizobakteri  dalam menghambat  pertumbuhan  P.  capsici,  dan  (2)  mengetahui  pengaruh perlakuan benih dengan rizobakteri dalam meningkatkan vigor benih dan pertumbuhan tanaman, serta dalam mengurangi kejadian penyakit busuk phytophthora. Penelitian ini terdiri atas  dua  tahap percobaan, menggunakan rancangan acak lengkap satu faktor. Percobaan pertama (in vitro) terdiri atas sembilan taraf yaitu tujuh kombinasi isolat rizobakteri, metalaksil,  dan tanpa perlakuan (kontrol).  Percobaan kedua  (di  rumah  kaca)  terdiri  atas  enam taraf  perlakuan  benih  yaitu  rizobakteri  ST116B,  CM8, ST116B  +  CM8, metalaksil,  kontrol  positif,  dan  kontrol  negatif.  Hasil  percobaan  in  vitro, semua perlakuan rizobakteri baik tunggal maupun yang dikombinasikan mampu menghambat pertumbuhan patogen  P.  capsici.  Persentase  daya hambat tertinggi  berturut-turut  ditunjukkan  oleh  isolat rizobakteri  CM8, kombinasi isolat  rizobakteri  ST116B  +  CM8,  dan  isolat  rizobakteri  ST116B. Perlakuan benih dengan rizobakteri ST116B, CM8, dan ST116B + CM8 tidak dapat meningkatkan vigor  benih,  namun  secara  nyata  meningkatkan pertambahan jumlah  daun  pada  minggu  ke  enam setelah  pindah  tanam, dan menurunkan  kejadian  penyakit  busuk  phytophthora. Tidak terdapat perbedaan  nyata  pengaruh  rizobakteri  yang  diaplikasikan secara tunggal  maupun  kombinasi  dua isolat. Rizobakteri ST116B disarankan untuk digunakan dalam perlakuan benih cabai sebelum tanam.</p><p>Kata kunci: metalaksil, penyakit busuk phytophthora, rizobakteri</p>

scholarly journals Peningkatan Pertumbuhan Tanaman Cabai dan Pengendalian Busuk Phytophthora melalui Biopriming Benih dengan Rizobakteri Asal Pertanaman Cabai Jawa Timur

2017 ◽  
Vol 8 (3) ◽  
pp. 171 ◽  
Author(s):  
Aulia Zakia ◽  
Satriyas Ilyas ◽  
Candra Budiman ◽  
Syamsuddin , ◽  
Dyah Manohara
Keyword(s):  
Plant Growth ◽  
Seed Treatment ◽  
Block Design ◽  
Phytophthora Capsici ◽  
Positive Control ◽  
Negative Control ◽  
Phytophthora Blight ◽  
Blight Disease ◽  
And Control ◽  
East West

<p align="center"><strong><em>ABSTRACT <br /></em></strong></p><p><em>The objectives of this study was to evaluate biopriming of chili seed with rhizobacteria to improve plant growth and control Phytophthora blight disease in a greenhouse. This experiment used three isolates of rhizobacteria, i.e. E1, E3C2 and F2B1, and isolate <span style="text-decoration: underline;">Phytophthora</span> <span style="text-decoration: underline;">capsici</span> (Cb6) isolated from the production center of chili in East Jawa. Laris variety from PT. East West was used in this experiment. This experiment used randomized block design with one factor, i.e. 11 levels of seed treatment (E1 rhizobacteria, E3C2 rhizobacteria, F2B1 rhizobacteria, E1+E3C2 rhizobacteria, E1+F2B1 rhizobacteria, E1+E3C2+F2B1 rhizobacteria, seed soaking in water, without soaking, metalaxyl, positive control and negative control). The result showed that seed treatment with combination of E1+F2B1 isolates when grown in nursery, significantly increased the height and number of leaves in chilli. Besides, seed treatment with F2B1 isolate and combination of E1+F2B1 isolates after transplanting were capable to improve plant growth and control Phytophthora blight disease in greenhouse.</em></p><p><em>Keywords: greenhouse, isolate rhizobacteria, <span style="text-decoration: underline;">Phytophthora</span> <span style="text-decoration: underline;">capsici</span></em></p><p align="center"><em>  <br /></em></p><p align="center"><strong>ABSTRAK <br /></strong></p><p>Tujuan penelitian ini ialah mengevaluasi perlakuan <em>biopriming</em> benih cabai dengan rizobakteri dalam meningkatkan pertumbuhan bibit dan mengendalikan kejadian busuk Phytophthora di rumah kaca. Perlakuan <em>biopriming</em> benih dengan rizobakteri menggunakan tiga isolat rizobakteri E1, E3C2 dan F2B1 dan isolat <em>Phytophthora capsici</em> Cb6 hasil eksplorasi pertanaman cabai Jawa Timur. Benih yang digunakan dalam percobaan merupakan benih varietas Laris produksi PT. East West. Percobaan menggunakan rancangan acak kelompok satu faktor, masing-masing perlakuan diulang empat kali, dengan 11 taraf perlakuan, antara lain R0+ (kontrol positif, benih direndam dalam PDB tanpa perlakuan rizobakteri dengan inokulasi <em>P. capsici</em>), R0- (kontrol negatif, benih direndam dalam PDB tanpa perlakuan rizobakteri dan tanpa inokulasi <em>P. capsici</em>), R1 (perlakuan benih dengan isolat E1), R2 (isolat E3C2), R3 (isolat F2B1), R4 (kombinasi isolat E1+E3C2), R5 (kombinasi isolat E1+F2B1), R6 (kombinasi isolat E1+E3C2+F2B1), R0RA (benih direndam dalam air 24 jam), R0TR (benih tanpa rendam), R0M (benih direndam dalam metalaksil). Tanah inokulum <em>P. capsici</em> diberikan 28 hari setelah pindah-tanam di sekitar pangkal batang tanaman cabai di bawah permukaan tanah. Hasil percobaan menunjukkan, perlakuan dengan kombinasi isolat E1+F2B1 saat persemaian di rumah kaca nyata meningkatkan tinggi dan jumlah daun tanaman cabai. Perlakuan benih dengan isolat F2B1 maupun kombinasi isolat E1+F2B1 setelah pindah-tanam di rumah kaca memiliki kemampuan meningkatkan pertumbuhan tanaman serta mengendalikan penyakit busuk Phytophthora. </p><p>Kata kunci: isolat rizobakteri,<em> Phytophthora capsici</em>, rumah kaca</p>

Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Fungicidal Activity ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

scholarly journals Aktivitas Antifungi Ekstrak Etanol Daun Eryngium foetidum L. Terhadap Pertumbuhan Xeromyces sp. secara in vitro

2019 ◽  
Vol 8 (2) ◽  
Author(s):  
Ines S Simatupang Elvi Rusmiyanto PW, Rikhsan Kurniatuhadi
Keyword(s):  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Activity Level ◽  
Piper Nigrum ◽  
Dilution Method ◽  
Randomized Design ◽  
Eryngium Foetidum ◽  
Completely Randomized Design

Eryngium foetidum is a plant commonly used a seasoning cuisine and has a variety of chemical compounds that potentially an antifungal. The ethanol extract Eryngium foetidum was tested against fungal isolate Xi.01. The isolat Xi.01 isolated from the pepper (Piper Nigrum) stem was identified as Xeromyces sp. This study aimed to determine the ability of the antifungal ethanol extract of Eryngium foetidum against Xeromyces sp. (Xi.01). This study used solid dilution method and completely randomized design using 18 treatments, i.e negative control, positive control, diethanolamide concentration of 2.5; 5; 7.5 and 10% combined with the ethanol extract concentration of Eryngium foetidum of 5; 10; 20 and 40%. The results showed 16 treatment combinations had the same low antifungal activity level in the range 13,59-22,40%

scholarly journals SKRINING FITOKIMIA DAN AKTIVITAS ANTIBAKTERI in vitro EKSTRAK HEKSANA-PETROLEUM ETER Artemisia cina Berg. ex Poljakov

Agric ◽  
2016 ◽  
Vol 27 (1) ◽  
pp. 30
Author(s):  
Elizabeth B.E Kristiani ◽  
Sri Kasmiyati ◽  
Maria Marina Herawati
Keyword(s):  
Antibacterial Activity ◽  
Petroleum Ether ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
E Coli ◽  
Randomized Design ◽  
Antibactrial Activity

<p>The objectives of this reserch were to determine the antibacterial activity of hexane-petroleum ether (1 : 1 v/v) extract of Artemisia cinaBerg. ex Poljakov on Escherichia coli and Staphylococcus aureus and its compounds. The design of the research was using completely Randomized Design in five concentration of extract that were 0, 50, 75, 100, 150, dan 200 mg/ml with four repliceted. Ethanol 60% was used as negative control and tetracycline as positive control. There were three steps of research. First step was extracted the plant using soxhlet method with hexane-petroleum ether (1:1 v/v). Second step was determined the antibacterial activity of hexane-petroleum ether (1:1 v/v) extract in various concentration of that extracts on E. Coli and S. aureus using agar diffusion method. Analysis of Variance (ANOVA) and was used to determined the significan different of diameter of inhibition between the treatments. Thirdsteps was phytochemical analysis of extract. The highest antibactrial activity on E. Coli was at 100 mg/ml whereas on S. Aureus at 150 mg/ml. That extract was contained flavonoid, alkaloid, essential oils, saponin, sterol, tritepene, hydrolized tannin, and coumarin.</p>

scholarly journals PENGARUH EKSTRAK Sargassum cristaefolium PADA MULTIPLIKASI Dendrobium antennatum Rchb.f SECARA IN VITRO

2020 ◽  
Vol 8 (1) ◽  
pp. 18
Author(s):  
Faisal Ansyarif ◽  
Mursal Ghazali ◽  
Aida Muspiah ◽  
Rina Kurnianingsih
Keyword(s):  
Culture Media ◽  
Positive Control ◽  
Negative Control ◽  
Growth Media ◽  
Extract Concentration ◽  
Tissue Culture Media ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Natural Cytokinin

The purpose of this study was to determine the potential and concentration of Sargassum cristaefolium extract as a natural cytokinin in tissue culture media of Dendrobium antennatum Rchb.f. This study is experimental with a completely randomized design, using several extract concentrations compared with the positive control (BAP 1.5 ppm) and negative control (MS0 media). Extract concentrations used 5 ppm, 10 ppm, 15 ppm, 20 ppm, and 25 ppm. Based on analysis of variance (ANOVA), the effect of Sargassum cristaefolium extract on the growth media significant on all parameters. Sargassum cristaefolium extracts caused different responses at certain levels of concentration. Extract concentration of 10 ppm was able to initiate the highest number of shoots and leaves compared to other extract concentrations, where as the concentration 20 ppm was able to accelerate and increase root growth.

scholarly journals EXPLORATION AND SELECTION OF RHIZOBACTERIA THAT INHIBIT PHYTOPHTHORA CAPSICI IN VITRO

2018 ◽  
Vol 18 (1) ◽  
pp. 83
Author(s):  
Aulia Zakia ◽  
Satriyas Ilyas ◽  
Candra Budiman ◽  
Syamsuddin . ◽  
Dyah Manohara
Keyword(s):  
Phytophthora Capsici ◽  
Culture Method ◽  
Healthy Plant ◽  
Dual Culture ◽  
Phytophthora Blight ◽  
Heated Sample ◽  
Resistant Varieties ◽  
Blight Disease ◽  
Selection Of

Exploration and Selection of Rhizobacteria that Inhibit Phytophthora capsici in vitro. Phytophthora capsici, a seed borne and the soil borne fungal pathogen is the cause of phytophthora blight on chili. The disease is difficult to control because of the resistant varieties unavailability in Indonesia. The aimed was to obtain isolates of rhizobacteria which has the ability to inhibit P. capsici in vitro. Rhizobacteria exploration was conducted in the chili production center in East Java (Malang, Batu, and Kediri) and West Java (Bogor). In one location, chili plant that had symptoms of phytophthora blight disease and a healthy plant next to it were chosen as samples to isolate P. capsici and the rhizobacteria. The rhizobacteria were isolated on NA, TSA, and TSAP (TSA with heated sample). Samples of diseased plants were used in isolation of P. capsici on V8 agar. The inhibition and compatibility of the rhizobacteria to inhibit P. capsici in vitro were tested by dual culture method. In this experiment, it was obtained 252 isolates of rhizobacteria and one isolate of P. capsici. Isolates of rhizobacteria with high to medium inhibition were E1, E3C2, and F2B1 respectively. All three isolates were then combined and tested against P. capsici in vitro. The highest inhibition was indicated by four isolate and combination of isolates, which were E1 isolate (58%), the combination of E1 + E3C2 isolates (58%), E1 + F2B1 (60%) and E1 + E3C2 + F2B1 (58 %).

scholarly journals Lime Peel Liquid (Citrus aurantifolia, Swingle) Inhibit Escherichia Coli In Vitro

2021 ◽  
Vol 4 (1) ◽  
pp. 63
Author(s):  
Devi Indah Sari ◽  
Retno Sri Wahjuni ◽  
Ratih Novita Praja ◽  
Budi Utomo ◽  
Faisal Fikri ◽  
...  
Keyword(s):  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Citrus Aurantifolia ◽  
E Coli ◽  
Randomized Design ◽  
Mueller Hinton ◽  
Lime Peel

The purpose of this study was to determine in vitro inhibition test of lime peel liquid (Citrus aurantifolia, Swingle) against E. coli. The E. coli isolate was cultured on Muller Hinton Agar (MHA) media. Antibacterial test in this study using diffusion method. Negative control (K-) using CMC-Na 0.1%. Positive control (K+) using oxytetracycline antibiotics. The treatments were given lime peel liquid (Citrus aurantifolia, Swingle) with concentrations of 100%, 75%, 50% and 25%, respectively. The study design using Completely Randomized Design (CRD) method, with six treatments and four repetitions. Data were analyzed by Kruskal-Wallis test, Moreover, the Post Hoc Mann-Whitney method. The inhibition zone analysis results were showed that K + had significant differences compared to all treatments K-, 100%, 75%, 50%, and 25%. K- was found to be significantly different from treatments 100%, 75%, 50% and 25%. The results showed that the lime peel liquid has the potential as an antibacterial activity against the growth of E. coli in vitro. Concentration of 25% has shown the presence of inhibitory zones on Mueller Hinton media that have been inoculated with lime peel liquid.

scholarly journals Potensi Bacillus licheniformis dan Streptomyces olivaceoviridis sebagai Penghambat Pertumbuhan Jamur Saprolegnia sp. Penyebab Saprolegniasis pada Ikan secara In Vitro [Potential of Bacillus licheniformis and Streptomyces olivaceoviridis as Inhibiting The Growth of Fungus Saprolegnia sp, Cause Saprolegniasis on Fish by Using In Vitro]

2019 ◽  
Vol 7 (2) ◽  
pp. 133
Author(s):  
Oktantia Frenny Anggani ◽  
Rahayu Kusdarwati ◽  
Hari Suprapto
Keyword(s):  
Bacillus Licheniformis ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Significant Difference ◽  
Streptomyces Olivaceoviridis ◽  
Tukey Honestly Significant Difference

Abstract Saprolegniasis is a fungal disease on fish caused by Saprolegnia sp, which is saprophyte, damaging healthy tissue and makes the immune system in fish deacreased. The uniquennes of Saprolegnia sp has the main components of the cell wall in the form of chitin that was instrumental in shaping the structure of the tip growth of fungal hyphae. Control of fungal pathogenic Saprolegnia sp can use chitinolytic microorganisms based on ability to produce chitinase for example using bacteria. This study aims to potential of Bacillus licheniformis and Streptomyces olivaceoviridis as inhibiting the growth of fungus Saprolegnia sp, cause saprolegniasis on fish by using in vitro. The research method is experimental with completely randomized design (CRD), which consists of four treatments with five replications. The experimental used is A (Saprolegnia sp and Bacillus licheniformis), B (Saprolegnia sp and Streptomyces olivaceoviridis), C negative control (Saprolegnia sp) and D positive control (Saprolegnia sp and Ketokonazol 2 %). The main parameters measured were observed inhibition zone on each treatment. Supporting parameters were observed is an observation of abnormal hpyphae structure after being induced by bacteria Bacillus licheniformis dan Streptomyces olivaceoviridis. Data were analyzed using analysis of variants (ANOVA) and to know the difference between treatments were determined by Tukey honestly significant difference (Tukey HSD) Test. The results showed that the potential of chitinolytic Bacillus licheniformis can provide a good barrier of 4,62 cm by 5,48 cm compared Streptomyces olivaceoviridis in inhibiting the growth Saprolegnia sp. Suggestions in this research is the need to further research on the value of chitinase Bacillus licheniformis and Streptomyces olivaceoviridis. Further research by using in vivo.

scholarly journals Anthelmintic Activity of Curcuma Aeruginosa Roxb Extract on Fasciola gigantica in Vitro

2020 ◽  
Vol 151 ◽  
pp. 01046
Author(s):  
Henni Vanda ◽  
Rizki Parindra ◽  
Muhammad Hambal ◽  
Farida Athaillah
Keyword(s):  
Methanolic Extract ◽  
Anthelmintic Activity ◽  
Positive Control ◽  
Negative Control ◽  
Fasciola Gigantica ◽  
Histopathological Study ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Phosphate Buffered Saline

Fasciola gigantica is a parasite that causes a disruption of the metabolism of fats, proteins, and carbohydrates, which interferes growth and causes death. Curcuma aeruginosa Roxb extract is one of the medicinal plants which has been used to treat several diseases. The aim of this study was to determine the effect of methanolic extract of C. aeruginosa Roxb on F. gigantica, including mortality time and histopathological changes that occurred after treatment. This study used a completely randomized design with five replications. The flukes were soaked in three different extract concentrations: 10% (T1), 25% (T2), and 50% (T3) (w/v). Phosphate buffered saline (PBS) solution was used as a negative control (C1) and albendazole as the positive control (C2). The mortality time of F. gigantica in each group was calculated, and the dead flukes were prepared for histopathological study. The data were analyzed by Analysis of variance. The results showed that C. aeruginosa extracts at the concentration of 10, 25 and 50% caused the death of the flukes within 75, 57 and 48 minutes, respectively., Histopathological observations showed that the extract caused breakage of tegument which is an important organ in the respiratory process and nutrient absorption. This study concluded that C. aeruginosa extract exhibited anthelmintic activity towards F. gigantica in vitro.

scholarly journals Peran Mikoriza Arbuskula pada Insidensi Penyakit Busuk Pangkal Batang Lada

2017 ◽  
Vol 12 (5) ◽  
pp. 178
Author(s):  
Halim Halim ◽  
Mariadi Mariadi ◽  
La Karimuna ◽  
Rachmawati Hasid
Keyword(s):  
Disease Incidence ◽  
Phytophthora Capsici ◽  
Positive Control ◽  
Negative Control ◽  
Control Treatment ◽  
Foot Rot ◽  
Important Constraint ◽  
Randomized Design ◽  
Rot Disease ◽  
Pepper Plants

Stem rot or foot rot disease caused by Phytophthora capsici is known as an important constraint on pepper cultivation. Research was conducted to determine the effect of arbuskula mycorhizal fungi (AMF) on incidence of foot rot disease of pepper seedlings.  The experiment was done in the net house and arranged using completed randomized design with 6 treatments, i.e. (1) soil infested by P. capsici (TPC) as negative control treatment, (2) sterilized soil (TS) as positive control treatment, (3) TPC with 5 g of AMF, (4) TPC with 10 g AMF, (5) TPC with 15 g AMF, and (6) TPC with 20 g AMF.   Observation involved plant height, number of shoots, disease incidence, the percentage of AMF infection on the roots of pepper plants, and pepper plants dependence on AMF. The results showed that the application of AMF at a dose of 20 g per 10 kg of soil effectively suppressed incidence of foot rot disease and improve plant growth

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