scholarly journals Peningkatan Pertumbuhan Tanaman Cabai dan Pengendalian Busuk Phytophthora melalui Biopriming Benih dengan Rizobakteri Asal Pertanaman Cabai Jawa Timur

2017 ◽  
Vol 8 (3) ◽  
pp. 171 ◽  
Author(s):  
Aulia Zakia ◽  
Satriyas Ilyas ◽  
Candra Budiman ◽  
Syamsuddin , ◽  
Dyah Manohara

<p align="center"><strong><em>ABSTRACT <br /></em></strong></p><p><em>The objectives of this study was to evaluate biopriming of chili seed with rhizobacteria to improve plant growth and control Phytophthora blight disease in a greenhouse. This experiment used three isolates of rhizobacteria, i.e. E1, E3C2 and F2B1, and isolate <span style="text-decoration: underline;">Phytophthora</span> <span style="text-decoration: underline;">capsici</span> (Cb6) isolated from the production center of chili in East Jawa. Laris variety from PT. East West was used in this experiment. This experiment used randomized block design with one factor, i.e. 11 levels of seed treatment (E1 rhizobacteria, E3C2 rhizobacteria, F2B1 rhizobacteria, E1+E3C2 rhizobacteria, E1+F2B1 rhizobacteria, E1+E3C2+F2B1 rhizobacteria, seed soaking in water, without soaking, metalaxyl, positive control and negative control). The result showed that seed treatment with combination of E1+F2B1 isolates when grown in nursery, significantly increased the height and number of leaves in chilli. Besides, seed treatment with F2B1 isolate and combination of E1+F2B1 isolates after transplanting were capable to improve plant growth and control Phytophthora blight disease in greenhouse.</em></p><p><em>Keywords: greenhouse, isolate rhizobacteria, <span style="text-decoration: underline;">Phytophthora</span> <span style="text-decoration: underline;">capsici</span></em></p><p align="center"><em>  <br /></em></p><p align="center"><strong>ABSTRAK <br /></strong></p><p>Tujuan penelitian ini ialah mengevaluasi perlakuan <em>biopriming</em> benih cabai dengan rizobakteri dalam meningkatkan pertumbuhan bibit dan mengendalikan kejadian busuk Phytophthora di rumah kaca. Perlakuan <em>biopriming</em> benih dengan rizobakteri menggunakan tiga isolat rizobakteri E1, E3C2 dan F2B1 dan isolat <em>Phytophthora capsici</em> Cb6 hasil eksplorasi pertanaman cabai Jawa Timur. Benih yang digunakan dalam percobaan merupakan benih varietas Laris produksi PT. East West. Percobaan menggunakan rancangan acak kelompok satu faktor, masing-masing perlakuan diulang empat kali, dengan 11 taraf perlakuan, antara lain R0+ (kontrol positif, benih direndam dalam PDB tanpa perlakuan rizobakteri dengan inokulasi <em>P. capsici</em>), R0- (kontrol negatif, benih direndam dalam PDB tanpa perlakuan rizobakteri dan tanpa inokulasi <em>P. capsici</em>), R1 (perlakuan benih dengan isolat E1), R2 (isolat E3C2), R3 (isolat F2B1), R4 (kombinasi isolat E1+E3C2), R5 (kombinasi isolat E1+F2B1), R6 (kombinasi isolat E1+E3C2+F2B1), R0RA (benih direndam dalam air 24 jam), R0TR (benih tanpa rendam), R0M (benih direndam dalam metalaksil). Tanah inokulum <em>P. capsici</em> diberikan 28 hari setelah pindah-tanam di sekitar pangkal batang tanaman cabai di bawah permukaan tanah. Hasil percobaan menunjukkan, perlakuan dengan kombinasi isolat E1+F2B1 saat persemaian di rumah kaca nyata meningkatkan tinggi dan jumlah daun tanaman cabai. Perlakuan benih dengan isolat F2B1 maupun kombinasi isolat E1+F2B1 setelah pindah-tanam di rumah kaca memiliki kemampuan meningkatkan pertumbuhan tanaman serta mengendalikan penyakit busuk Phytophthora. </p><p>Kata kunci: isolat rizobakteri,<em> Phytophthora capsici</em>, rumah kaca</p>

2015 ◽  
Vol 6 (1) ◽  
pp. 1 ◽  
Author(s):  
Farih Najah Rosadiah ◽  
Satriyas Ilyas ◽  
Dyah Manohara

<p>ABSTRACT</p><p>Seed  treatment  of  hot  pepper  using  rhizobacteria  is  an  alternative  to fungicide  use  in controlling  phytophthora  rot  disease.  The  objectives  of this  research  were  to  evaluate: (1)  the effectiveness of rhizobacteria isolates in inhibiting Phytophthora capsici growth and (2) the effect of seed treatment using rhizobacteria on  plant growth,  and  incidence of phytophthora blight  disease. This  research  consisted  of  two  experiments,  all experiments were  arranged in  completely randomized design using one factor. The first experiment (in vitro) consisted of nine levels i.e. sevencombination isolates of rhizobacteria, metalaxyl and control. The second experiment (in the green house)  consisted  of  six  levels  of  seed  treatments  i.e.  ST116B rhizobacteria,  CM8  rhizobacteria, ST116B + CM8  rhizobacteria, metalaxyl, positive control  and negative control. Results of in vitro experiment  showed that  all  rhizobacteria,  single  or  combinations,  were  able  to  inhibit  P. capsicigrowth. The  highest  inhibition  were  shown  by  CM8,  ST116B  + CM8, and ST116B  consecutively.Seed treatments of hot pepper using ST116B, CM8, and ST116B + CM8 rhizobacteria increased the number  of  leaves  6  weeks after  transplanting  and  reduced  the  incidence  of  phytophthora blight disease.  There were no significant  differences whether  the rhizobacteria was applied  singly  or  in combination  of  the  two.  Rhizobacteria  ST116B was  suggested  for  pepper  seed  treatment  before planting.</p><p>Keywords: metalaxyl, phytophthora blight disease, rhizobacteria</p><p> </p><p>ABSTRAK</p><p>Perlakuan  benih  cabai  menggunakan  rizobakteri  merupakan  alternatif pengganti  fungisida dalam  mengendalikan  penyakit  busuk  phytophthora, yang  disebabkan  oleh  cendawan  patogen Phytophthora  capsici.  Penelitian ini bertujuan (1) mengevaluasi keefektifan  kombinasi  isolat rizobakteri  dalam menghambat  pertumbuhan  P.  capsici,  dan  (2)  mengetahui  pengaruh perlakuan benih dengan rizobakteri dalam meningkatkan vigor benih dan pertumbuhan tanaman, serta dalam mengurangi kejadian penyakit busuk phytophthora. Penelitian ini terdiri atas  dua  tahap percobaan, menggunakan rancangan acak lengkap satu faktor. Percobaan pertama (in vitro) terdiri atas sembilan taraf yaitu tujuh kombinasi isolat rizobakteri, metalaksil,  dan tanpa perlakuan (kontrol).  Percobaan kedua  (di  rumah  kaca)  terdiri  atas  enam taraf  perlakuan  benih  yaitu  rizobakteri  ST116B,  CM8, ST116B  +  CM8, metalaksil,  kontrol  positif,  dan  kontrol  negatif.  Hasil  percobaan  in  vitro, semua perlakuan rizobakteri baik tunggal maupun yang dikombinasikan mampu menghambat pertumbuhan patogen  P.  capsici.  Persentase  daya hambat tertinggi  berturut-turut  ditunjukkan  oleh  isolat rizobakteri  CM8, kombinasi isolat  rizobakteri  ST116B  +  CM8,  dan  isolat  rizobakteri  ST116B. Perlakuan benih dengan rizobakteri ST116B, CM8, dan ST116B + CM8 tidak dapat meningkatkan vigor  benih,  namun  secara  nyata  meningkatkan pertambahan jumlah  daun  pada  minggu  ke  enam setelah  pindah  tanam, dan menurunkan  kejadian  penyakit  busuk  phytophthora. Tidak terdapat perbedaan  nyata  pengaruh  rizobakteri  yang  diaplikasikan secara tunggal  maupun  kombinasi  dua isolat. Rizobakteri ST116B disarankan untuk digunakan dalam perlakuan benih cabai sebelum tanam.</p><p>Kata kunci: metalaksil, penyakit busuk phytophthora, rizobakteri</p>


2016 ◽  
Vol 21 (3) ◽  
pp. 265
Author(s):  
Evi Silfia Yusuf ◽  
Donald Sihombing ◽  
Wahyu Handayati ◽  
Waqiah Nuryani ◽  
Saepuloh

Kutudaun Macrosiphoniela sanborni merupakan salah satu hama penting yang menimbulkan kerugian yang cukup serius pada budidaya krisan. Untuk mengendalikannya petani biasa menggunakan pestisida kimia sintetis. Selain itu pengendalian hayati berpotensi dapat menekan hama. Beauveria bassiana merupakan agens pengendali hayati  yang memiliki potensi besar untuk mengendalikan beberapa hama penting tanaman hias. Biorama 1, 2, dan 3 merupakan bioinsektisida berbahan aktif  B. bassiana dengan kepadatan1010 konidia per g bahan pembawa dengan bahan pembawa yang berbeda (tepung jagung, sekam, dan talk) yang dibuat di Laboratorium Biokontrol, Balai Penelitian Tanaman Hias (Balithi). Bioinsektisida tersebut terbukti efektif mengendalikan trips pada tanaman krisan. Penelitian bertujuan mengetahui efektivitas bioinsektisida Biorama 1, 2, dan 3 dalam mengendalikan kutudaun krisan. Penelitian dilaksanakan di Rumah Plastik Balithi Segunung sejak bulan Juli hingga November 2008. Bahan yang digunakan dalam penelitian ini ialah Dendranthema grandiflora kultivar Sakuntala. Perlakuan yang diuji yaitu Biorama 1 (1010 konidia/g tepung jagung), Biorama 2 (1010 konidia/g arang sekam), dan  Biorama 3 (1010 konidia/g talk) masing-masing dengan konsentrasi aplikasi 5 g/l, suspensi B. bassiana dengan kepadatan 1010 konidia/ml, Natural BVR adalah bioinsektisida komersial berbahan aktif B. bassiana (1010 konidia/g bahan pembawa) dengan konsentrasi aplikasi 5 g/l sebagai kontrol positif dan air sebagai kontrol negatif. Penelitian disusun menggunakan rancangan acak kelompok terdiri atas enam perlakuan dan lima ulangan. Hasil penelitian menunjukkan bahwa bioinsektisida Biorama 1, 2, dan 3  efektif mengendalikan kutudaun M. sanborni dan efektivitasnya tidak berbeda nyata dibandingkan dengan Natural BVR dan suspensi  Beauveria. Perlakuan tersebut mampu menekan serangan M. sanborni, masing-masing dengan 68,58,  60,59, dan  54,37 % secara berurutan. Bioinsektisida memberikan pengaruh nyata terhadap mortalitas kutudaun. Biorama 1 dan 2 menunjukkan keefektifan yang paling tinggi  (22,30 dan 24,20%) dalam menekan kerusakan bunga  dibandingkan dengan perlakuan lain dan kontrol. Implikasi dari penelitian ini ialah Biorama memiliki potensi yang besar diaplikasikan sebagai pestisida hayati ramah lingkungan pada budidaya krisan.<br /><br /><br /><br />Macrosiphoniela sanborni is one of the important pests causing serious losses on Chrysanthemum. Beauveria bassiana is a biological control agents, which has great potential to control several important pests on ornamental plants. Biorama 1, 2, and 3 are bioinsecticides containing B. bassiana as an active ingredient and made in Biological Control Laboratory of Indonesian Ornamental Crops Research Institute (IOCRI). The bioinsecticides were successfully tested and gave significant effect on controlling Chrysanthemum thrips. The study was aimed to determine the effectiveness of Biorama 1, 2, and 3 on controlling Chrysanthemum aphids. The experiment was conducted at the Plastichouse of  IOCRI from July to November 2008. Plant material used in this study was Dendranthema grandiflora cv. Sakuntala. The treatments tested were Biorama 1 (1010 conidia/g corn powder), Biorama 2 (1010 conidia/g activated-carchoal), and  Biorama 3 (1010 conidia/g talk) respectively with a concentration of 5 g/l, B. bassiana suspension with a density of 1010 conidia/ml, Natural BVR is a bioinsecticide with active ingredient B. bassiana, which has been commercialized as positive control in concentration 5 g/l and water as negative control. The experiment was arranged by a randomized complete block design consisted of  six treatments and five replications. The results showed that Biorama 1, 2, and 3 were effective to control  M. sanborni and the results did not give significant difference compared to  Natural BVR and Beauveria suspension. They reduced Chrysanthemum aphids up to 68.58, 60.59, and 54,37% respectively. Bioinsecticide provide real impact on the mortality of aphids. The Biorama 1 and 2 indicated the highest effectiveness (22.3 and 24.2% respectively) on lowering to flower damage compared to other treatments and control. This results indicated that Biorama have high potential applied as an environmental friendly biopesticide on Chrysanthemum cultivation.


2013 ◽  
Vol 162 (6) ◽  
pp. 367-376 ◽  
Author(s):  
Kyaw W. Naing ◽  
Muhammad Anees ◽  
Xuan H. Nguyen ◽  
Yong S. Lee ◽  
Seung W. Jeon ◽  
...  

JSMARTech ◽  
2021 ◽  
Vol 2 (2) ◽  
pp. 048-054
Author(s):  
Annisatul Hakimah ◽  
◽  
Nour Sjakoer ◽  
Nurul Mubarakati ◽  
◽  
...  

This study determined the effect of Mango mistletoe Methanolic Extract (MMME) on the cardiac's histopathological profile of hypertensive rats (DOCA-Salt) treated. The MMME was treated on fifty rats for 14 days, divided into five treatments: negative control, positive control, doses 50, 100, and 200 mg/kg BW with five replications. One-way ANOVA analysis was used, namely JAMOVI with version 1.1.9.0, and for cell calculation, diameter using the IMAGE J application. Results showed that there were no significant differences in the histopathological profile of the cardiac in hypertensive rats (DOCA-Salt) treated with MMME for 14 days on the diameter of the heart muscle cells between the control (+) and control groups (-), treatments 1, 2, and 3. This is evidenced by the analysis of p-value> 0.05, namely 0.187 millimeters. Therefore, we concluded that MMME does not affect the diameter of heart/cardiac organ muscle cells. However, there was a significant difference in the amount of necrosis in the cardiac of hypertensive rats between control (+) and control (-) groups, treatments 1, 2, and 3. Based on the results, MMME reduces the number of necrosis in the heart/cardiac organ.


2021 ◽  
Vol 883 (1) ◽  
pp. 012049
Author(s):  
L D Indarwati ◽  
E Sulistyaningsih ◽  
B Kurniasih

Abstract Shallot is a horticulture crop with a fibrous root system, which is susceptible to water deficit, particularly in the bulb formation stage. This study was carried out to examine the effects of salicylic acid (SA) and biosilica (Si) exogenous induction on plant growth of shallot grown under water deficit in plastic baskets. A factorial 4×2 experiment was laid out in a randomized complete block design with four blocks. The first factor of treatments was a four-level exogenous induction, i.e., 0.5mM SA, 6mM Si, combination (0.5mM SA and 6mM Si), and control (0 without SA and Si). The second factor was a level of interval irrigations i.e, one-day interval and three-day interval. The results showed that the treatment of combination 0.5mM SA and 6mM Si can maintained a plant height and the number of leaves was better than the control under water deficit. Avoidance was one of the mechanisms of shallot in dealing with water deficit, namely by reducing the stomatal density. Decreased stomatal density was negatively correlated with water use efficiency.


2020 ◽  
Vol 4 (5) ◽  
pp. 140-147
Author(s):  
Sri Sulami Endah Astuti

Ascariasis is an intestinal infection caused by the parasitic worm Ascaris lumbricoides. Starfruit leaf (Averrhoa bilimbi) and leaf anting-anting (Acalypha indica L) is a plant that is often used as a worm remedy in the community. Starfruit leaves (Averrhoa bilimbi) and Leaf anting-anting (Acalypha indica L) contain several compounds that have the potential as anthelmintics, namely saponins, tannins and flavonoids. The purpose of this study was to determine the effect of ethanol extention of starfruit leaf leaves (Averrhoa bilimbi) and leaf anting-anting (Acalypha indica L) as anthelmintic on the time of death of Ascaris suum Goeze worms in vitro. The method in this study is experimental with post test only group design. Test animals from the study are Ascaris suum. The study was conducted at the Parasitology Laboratory, Medical Laboratory Department, Poltekkes Kemenkes Surabaya in 2019. This study used 6 treatment groups, NaCl 0.9% as a negative control and pirantel pamoate 0.25% as a positive control and ethanol extract of wuluh starfruit leaf and ethanol extract of Anting-anting with concentrations of leaves. 40%, 60%, 80% and 100%. Data were analyzed using the Kolmogrov-Smirnov test, the Anova two way Block Design test, then continued using the Post Hoc test to determine differences between blocks and between treatments, against the time of Ascaris suum worm's death. The average time of death of Ascaris suum caused by ethanol extract of starfruit leaves concentration of 40% for 492.3 minutes and ethanol extract of leaf anting-anting 396.5 minutes, concentration of 60% ethanol extract of wuluh starfruit leaves for 278.5 minutes and leaf extract ethanol leaf anting-anting for 283.5 minutes, 80% concentration of ethanol extract of wuluh starfruit leaf for 141.8 minutes and ethanol extract of anting-anting leaf for 174 minutes, 100% concentration of ethanol extract of wuluh starfruit leaves for 66.75 minutes and extract ethanol leaf anting-anting for 92 minutes. So it can be concluded that the ethanol extract of starfruit leaves has an anthelmintic effect on the Ascaris suum worm more optimum than the ethanol extract of the Anting-anting leaves, because the time required is close to positive control that is 60 minutes. Keywords: anthelmintic; Ascaris suum; starfruit leaf; anting-anting leaf


2016 ◽  
Vol 37 (4Supl1) ◽  
pp. 2677 ◽  
Author(s):  
Paula Rodrigues Oliveira ◽  
Fabiana Ramos Santos ◽  
Eduardo Ferreira Duarte ◽  
Gustavo Silva Guimarães ◽  
Nadiessa Sartori Carvalho Mattos ◽  
...  

This study aimed to test the effects of dietary Aloe vera and Symphytum officinale extracts added separately or in combination with symbiotics on the performance, nutrient utilization, serum biochemical parameters, biometrics, and intestinal histomorfometry of broilers. The experiment had a randomized block design with five treatments and six replicates of ten broilers each. Treatments were as follows: negative control and positive control (diet without and with antibiotic, respectively); 0.2% Aloe vera (AV); 0.2% Symphytum officinale (S); 0.2% functional supplement, composed of symbiotics fermented in Aloe vera and comfrey plant extracts (S+PE). At seven days of age, FI of birds fed the Aloe vera extracts diets were lower than that observed for birds consuming the diet with Symphytum officinale extract and S+PE. Broiler performance remained unaffected by treatments at others ages evaluated. At 10 to 14 days of age the lowest ADCDM ADCCP was shown in group feed NC. The highest ADCCP was observed in PC control group and in diets supplemented with Aloe vera and S+PE. Serum levels of cholesterol, triglycerides, and phosphorus were affected by addition of extracts at seven, 21, and 35 days of age. The longest duodenal villi were observed in broilers fed S+PE diets at seven days of age. Aloe vera and Symphytum officinale extracts and symbiotics can be used in broiler diets as an alternative to growth-promoting antibiotics.


2014 ◽  
Vol 14 (1) ◽  
pp. 57-63
Author(s):  
Andi Khaeruni ◽  
Abdul Rahim ◽  
Syair Syair ◽  
Adriani Adriani

ABSTRACTInduced resistance to bacterial leaf blight disease in rice field by indigenous rhizobacteria.  Bacterial leaf blight is the most important disease on paddy at Southeast Sulawesi. Utilization of biological agents that induce plant resistance is an alternative tool to control bacterial leaf blight disease on paddy. The aim of  the experiment was obtain rhizobacteria that were able to stimulate the growth of paddy plants as well induce plant resistance towards bacterial leaf blight in the field. All experiment units were arranged with a factorial design in a randomized complete block design. The first factor was the rhizobacteria isolates, consisting 4 treatments, i.e: without rhizobacteria (R0), isolate P11a (R1), isolate PKLK5 (IR2), and  mixture P11a and PKLK5 (R3), the second factor is paddy varieties, (V1): IR64 variety, (V2): Cisantana variety. The pathogen was inoculated on leaf when 45 day after crop.  Weekly observation of disease severity, vegetative plant growth (leaf and stem numbers), and yield were conducted. The results showed that the 10 isolates of rhizobacteria tested were able to induce plant resistance toward bacterial leaf blight, stimulated vegetative growth as well as increased yield of paddy plant. Rhizobacteria application could increase the resistance of paddy toward Xanthomonas oryzae pv. oryzae. The application rhizobacteria could increase the vegetative plant growth, application  mixture P11a and PKLK5 isolates showed higher resistance than single application in terms of plant growth and yield, both IR64 and Cisantana varietes


2017 ◽  
Vol 1 (2) ◽  
pp. 97
Author(s):  
Diah Sudiarti

This study aimed at determining the effectiveness of biofertilizer with different concentrations on plant growth of soybean “edamame” (glycin max). Biofertilizer in this study consists of microbial consortia (lactobacillus, pseudomonas, bacillus, saccharomyces, rhizobium, azotobacter, azospirillum, and cellulomonas). The treatment consists of 3 biofertilizer concentrations (25%, 50%, dan 75%), as well as the negative control and positive control (100% chemical fertilizer equivalent 5g/plant). The result showed that treatment b3 (biofertilizer 75%) gave better results on plant growth compared with other treatments.


2019 ◽  
Vol 97 (9) ◽  
pp. 3907-3919 ◽  
Author(s):  
Hang Lu ◽  
Imke Kühn ◽  
Mike R Bedford ◽  
Hayley Whitfield ◽  
Charles Brearley ◽  
...  

Abstract The objective of this present study was to determine the effects of phytase dosing on growth performance, mineral digestibility, phytate breakdown, and the level of glucose transporter type 4 (GLUT4) in muscle plasma membranes of weanling pigs. A total of 160 barrows were used in a randomized completely block design and assigned to 4 treatments for a 7-wk study. Depending on the feeding phase, diets differed in dietary calcium (Ca) and phosphorus (P) levels (positive control [PC]: 8 to 6.8g/kg Ca; 7.3 to 6.3 g/kg P; negative control [NC]: 5.5 to 5.2 g/kg Ca; 5.4 to 4.7 g/kg P). NC diets were supplemented with phytase at 0 (NC); 500 (NC + 500 FTU); or 2,000 FTU/kg (NC + 2,000 FTU) phytase units/kg. Blood was collected after fasting (day 48) or feeding (day 49) for measurement of plasma inositol concentrations. On day 49, 2 pigs per pen were euthanized, and duodenal and ileal digesta samples were collected to determine inositol phosphates (InsP6-2) concentrations. High phytase supplementation increased BW on days 21, 35, and 49 (P < 0.05). Over the entire feeding period, ADG, ADFI, and feed efficiency were increased by NC + 2,000 FTU compared with the other treatments (P < 0.05). Postprandial plasma inositol concentration was increased in NC + 2,000 (P < 0.01), but there was only a tendency (P = 0.06) of a higher fasting plasma inositol concentration in this group. Inositol concentrations in the portal vein plasma (day 49) were not different among treatments. Duodenal digesta InsP5 and InsP6 concentrations were similar in PC and NC, but higher in these 2 treatments (P < 0.05) than those supplemented with phytase. Phytase supplementation decreased InsP6-4, resulting in increased InsP3-2 and myo-inositol concentrations. Similar effects were found in ileal contents. Compared with NC, phytase supplementation resulted in greater cumulative InsP6-2 disappearance (93.6% vs. 72.8% vs. 25.0%, for NC + 2,000 FTU, NC + 500 FTU and NC, respectively, P < 0.01) till the distal ileum. Longissimus dorsi muscle plasma membrane GLUT4 concentration was increased by NC + 2,000 FTU (P < 0.01) compared with NC. In summary, high phytase supplementation increased growth performance of nursery pigs. The higher myo-inositol release from phytate could contribute to the increased expression of GLUT4 in muscle plasma membranes. Further investigation is needed to determine whether this is associated with enhanced cellular glucose uptake and utilization.


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