scholarly journals The effect of aqueous extract of the roots of Salep plants on the serum concentration of FSH and estrogen hormone in female rats

2015 ◽  
Vol 13 (2) ◽  
pp. 39-44
Author(s):  
hossein Kargar jahromi ◽  
kavous solhjo ◽  
keramat allah solhjo ◽  
zahra Kargar jahromi ◽  
anahita ebrahimian ◽  
...  
Keyword(s):  
Serum Concentration ◽  
Aqueous Extract ◽  
Female Rats

scholarly journals The effect of aqueous extract of the roots of Salep plants on the serum concentration of progesterone and LH hormone in female rats

2015 ◽  
Vol 13 (1) ◽  
pp. 21-26
Author(s):  
kavous solhjo ◽  
hossein Kargar jahromi ◽  
keramat allah solhjo ◽  
zahra Kargar jahromi ◽  
zahra Khabbaz Kherameh ◽  
...  
Keyword(s):  
Serum Concentration ◽  
Aqueous Extract ◽  
Female Rats

Effects of the aqueous extract of Dicliptera verticillata on fertility and different stages of gestation in female rats

Zygote ◽  
2021 ◽  
pp. 1-7
Author(s):  
Stéphanie Ouabo Meguem ◽  
Landry Lienou Lienou ◽  
Marie Stéphanie Chekem Goka ◽  
Richard Simo Tagne ◽  
Didiane Mefokou Yemele ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Aqueous Extract ◽  
Implantation Rate ◽  
The Other ◽  
Female Rats ◽  
High Dose ◽  
Immature Female ◽  
Implantation Sites ◽  
Parameters Analysis ◽  
Hepatic Proteins

Summary Dicliptera verticillata is a medicinal plant traditionally used in western Cameroon to cure female infertility. This experiment was designed to assess the effects of the aqueous extract of Dicliptera verticillata (AEDv) on fertility and gestation in female rats. Oral increasing doses of AEDv were administered to immature female rats over 20 d. After this time, some animals were mated with fertile males and some fertility parameters were assayed; the other animals were euthanized for preliminary toxicity parameters analysis. The effects of AEDv on the different stages of gestation were assayed on selected animals previously controlled for estrous cycle regularity and mated. AEDv led to an increase in serum, uterine and ovarian proteins as well as in ovarian and uterine weights (P < 0.05) in immature female rats. Hepatic proteins significantly decreased (P < 0.01) in high dose-treated animals (50 and 100 mg/kg) compared with controls. The number of implantation sites and the fertility rate were significantly lower (P < 0.05), while the antifertility activity increased significantly (P < 0.05) in treated rats compared with controls. When administered from the 1st to the 5th day of pregnancy, AEDv led to a decrease of more than 60% in the implantation rate in high dose-treated rats (50, 100, and 400 mg/kg). From the 6th to the 9th day, the implantation, gestation rates and the number of fetuses decreased significantly in all treated groups. From the 11th to the 20th day, a 50% resorption and decrease in gestation rate were reported in 50 mg/kg dose-treated animals. AEDv possesses weak contraceptive and abortifacient effects during pregnancy.

scholarly journals Subchronic toxicity of aqueous extract of Alstonia boonei de wild. (apocynaceae) stem bark in normal rats

2015 ◽  
Vol 3 (1) ◽  
pp. 5 ◽  
Author(s):  
Barnabé Lucien Nkono Ya Nkono ◽  
Selestin Dongmo Sokeng ◽  
Paul Désiré Dzeufiet Djomeni ◽  
Frida Longo ◽  
Pierre Kamtchouing
Keyword(s):  
Aqueous Extract ◽  
Biochemical Study ◽  
Female Rats ◽  
Male Rats ◽  
Control Group ◽  
Control Male ◽  
Control Female ◽  
Hematological Analysis ◽  
Wbc Count ◽  
Dose Dependent

<p><strong>Methodology:</strong> Wistar rats were randomly assigned into eight groups of five animals each: four male groups and four female groups. Each sex group had a control group receiving distilled water and three test groups receiving 200, 500 and 1000mg/kg respectively. Animal’s body weights were recorded on the first day and once a week for the four experiment weeks. The hematological analysis included total WBC count, total RBC count, Hb, %HCT, MCV, MCH and MCHC. Biochemical/serum profile studies include TG, TC, ALT, AST, urea and TP. Tissue specimens of the liver, kidney and lung were subjected to histological examination using standard hematoxylin-eosin staining.</p><p><strong>Results:</strong> In male rats, aqueous extract showed significant decreases in relative weight of liver with extreme significance P&lt;0.001 at a dose of 200mg/kg (vs. control group), P&lt;0.001 of lung at all the doses, P&lt;0.05 (200 and 500mg/kg) and P&lt;0.01 (1000mg/kg) in heart weight. In relative kidney weight, only the dose of 1000mg/kg showed a significant increase vs. normal control male rats. Unlike male rats, only relative kidney weight in female rats was significantly different from the control group in a dose-dependent manner. The aqueous extract treated male groups showed significant increases P&lt;0.001 (1000mg/kg) of total WBC count and MCHC, significant decreases of %HTC (dose response manner), P&lt;0.05 total RBC count (at doses of 500 and 1000mg/kg) and Hb P&lt;0.01 (500mg/kg) vs. normal male rats. In female rats, the haematological study showed significant increase P&lt;0.01 of total WBC count (at the doses of 500 and 1000mg/kg), significant decreases P&lt;0.05 and P&lt;0.01 of total RBC respectively at the doses of 200 and 1000mg/kg, significant decrease of Hb with extreme significance P&lt;0.001 at the dose 1000mg/kg, %HTC also decrease dose response manner vs. control female rats. Biochemical study showed in male rats significant decreases in level of TG P&lt;0.001 (at the doses of 200 and 500mg/kg) and urea, although it showed any dose-dependent effect vs. control male rats. AST also decreases (P&lt;0.05) in male rats at the dose of 200mg/kg but significantly increase P&lt;0.001 at the dose of 500mg/kg. In the female rats, biochemical study revealed significant increases in level of TG P&lt;0.001 and urea P&lt;0.01 at the dose of 200mg/kg and significant decreases in level of TG P&lt;0.01, AST P&lt;0.05 and urea P&lt;0.05 at the dose of 500mg/kg (vs. control female rats). Microscopically, there were mild hepatic and renal tissue injuries supporting the hematological analysis.</p><p><strong>Conclusion:</strong> The results indicated that aqueous extract of <em>Alstonia boonei</em> De Wild is toxic in high doses.</p>

scholarly journals The Effect of an aqueous root extract of Iranian native edible asparagus (Asparagus officinalis L.) on the serum concentration of progesterone and LH hormone in female rats.

2015 ◽  
Vol 13 (2) ◽  
pp. 1-7
Author(s):  
hossein kargar jahromi ◽  
Hojatollah Karimi Jashni ◽  
zahra kargar jahromi ◽  
zahra Khabbaz Kherameh ◽  
◽  
...  
Keyword(s):  
Serum Concentration ◽  
Asparagus Officinalis ◽  
Female Rats ◽  
Root Extract ◽  
Aqueous Root Extract

Acute and sub-chronic toxicity of the aqueous extract of Ficus vogelii (Miq.) Miq. stem bark in rats

2021 ◽  
Vol 10 (2) ◽  
pp. 89-97
Author(s):  
EL Lappa ◽  
◽  
C Bogning Zangueu ◽  
EL Nguemfo ◽  
JJ Kojom Wanche ◽  
...  
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Chronic Toxicity ◽  
Hematological Parameters ◽  
Lethal Dose ◽  
Relative Weight ◽  
Stem Bark ◽  
The Body ◽  
Female Rats ◽  
Male Rats

Ficus vogelii is a medicinal plant mainly found in tropical Africa and reported to treat inflammatory complaints. This study aims to evaluate the acute and sub-chronic toxicity of the aqueous extract of Ficus vogelii stem bark in wistar rats. For acute study, aqueous extract at a single dose of 5000 mg/kg body weight was administered to female rats and observed for 14 days. In the sub-chronic study, the extract was administered daily to both sex rats at the doses of 100, 200, 400, and 600 mg/kg body weight for 28 consecutive days. Body weight was measured weekly, while hematological, biochemical, and histopathological parameters were analyzed after euthanize. Aqueous extract of Ficus vogelii at all tested doses didn’t produced any mortality or significant change on the body weight and relative weight of rats on acute and sub-chronic studies. The lethal dose 50 was estimated greater than 5000 mg/kg (DL50˃5000 mg/kg). Hematological parameters were recorded non-significant in all treated rats. Aqueous extract at 600 mg/kg significantly changed transaminases and alkaline phosphatase activities, these changes were reversible in satellites. The concentrations of bilirubin was increased at 200 and 600 mg/kg in male rats, at 100, 400 mg/kg in female rats. The levels of lipids markers didn’t changed, except the significant decrease of LDL-cholesterol. Histological examination didn’t showed any change in the architecture of the liver and kidney of rats treated compared to control. Thus aqueous extract of Ficus vogelii stem bark didn’t produced adverse effects in rats after oral acute and sub-chronic treatment.

Effects of systemic administration or intrabursal injection of serotonin on puberty, first ovulation and follicular development in rats

2013 ◽  
Vol 25 (8) ◽  
pp. 1105 ◽  
Author(s):  
M. J. Moran ◽  
M. E. Ayala ◽  
E. Gallegos ◽  
J. Romero ◽  
R. Chavira ◽  
...  
Keyword(s):  
Serum Concentration ◽  
Follicular Development ◽  
Systemic Administration ◽  
Female Rats ◽  
Subcutaneous Route ◽  
Vaginal Opening ◽  
Serum Concentrations ◽  
Neuroendocrine Mechanism

To elucidate the role of serotonin in the onset of puberty, the effects of both systemic and in-ovarian bursa administration of serotonin on the neuroendocrine mechanism that modulates the onset of puberty, follicular development and first ovulation were evaluated. Two experiments were carried out. For the first, 25 or 37.5 mg kg–1 of bodyweight of serotonin creatinine sulfate was administered by a subcutaneous route to 30-day-old female rats. In the second experiment, serotonin creatinine sulfate was administered directly into the ovarian bursa of 34-day-old female rats. Systemic administration of 25 or 37.5 mg kg–1 of serotonin creatinine sulfate induced a delay in the ages of vaginal opening and first vaginal oestrus, a decrease in the number of ovulating animals, and serum concentrations of FSH, LH, oestradiol and progesterone. An increase in the number of Class 3 (>500 μm) and atretic follicles was observed in the ovaries of these animals. The administration of serotonin creatinine sulfate in the ovarian bursa did not modify the onset of puberty and ovulation, but a reduced serum concentration of oestradiol was observed. Our results suggest that serotonin acts on the components of the hypothalamus–hypophysis–ovary axis by modulating follicular development, ovarian functions and the onset of puberty.

scholarly journals Therapeutic Effects ofCortex acanthopanacisAqueous Extract on Bone Metabolism of Ovariectomized Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Zhiguo Zhang ◽  
Jiazi Dong ◽  
Meijie Liu ◽  
Yan Li ◽  
Jinghua Pan ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Interleukin 1 ◽  
Inhibitory Effect ◽  
Control Agent ◽  
The Body ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Therapeutic Effects ◽  
Mineral Density ◽  
Ovx Rats

The aim of this study was to evaluate effects of aqueous extract fromCortex acanthopanacis(CAE) on osteoporosis rats induced by ovariectomy (OVX) using aqueous extract fromFolium Epimedii(FEE) as positive control agent. Three-month-old female rats that underwent OVX were treated with CAE. After 12 weeks, bone mineral density (BMD) and indices of bone histomorphometry of tibia were measured. Levels of protein and mRNA expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL) in tibia were evaluated. In addition, the serum concentrations of osteocalcin (OC), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), calcitonin (CT), and parathyroid hormone (PTH) were determined. Administration of CAE significantly prevented OVX-induced rats from gain of the body weight. Treatment with CAE increased bone mass remarkably and showed a significant inhibitory effect on bone resorption by downregulating significantly the expression of RANKL in tibia of OVX rats. Meanwhile, treatment of CAE significantly reduced serum level of IL-1βand increased level of CT in OVX rats. This suggests that CAE has the potential to be used as an alternative therapeutic agent for postmenopausal osteoporosis.

scholarly journals Acute and subacute toxicity assessment of an aqueous extract of Crotalaria retusa (Fabaceae) in Swiss mice and Wistar rats

2021 ◽  
Vol 11 (6) ◽  
pp. 94-100
Author(s):  
Lane Adrien GOH BI ◽  
Noel Kouame TOTO ◽  
Ouga Stanislas ZAHOUI ◽  
Yomalan KASSI ◽  
Semi Anthelme NENE Bi ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Wistar Rat ◽  
Toxicity Assessment ◽  
Female Rats ◽  
Male And Female ◽  
Subacute Toxicity ◽  
Swiss Mice ◽  
Aerial Parts ◽  
Male And Female Rats

The present study is a contribution to the enhancement of Ivorian traditional medicine, by carrying out bio-tolerance tests of an aqueous extract of the aerial parts of Crotalaria retusa (EACr) in mice and rats according to the guidelines of the Organization for Economic Co-operation and Development (OECD) 423 and 407, respectively. The doses of 2000 and 5000 mg/kg of body weight (BW) of the extract were used for acute toxicity. For subacute toxicity which lasted 28 days, doses of 500, 1000, and 2000 mg/kg BW have been used. The administration of the single doses of 2000 and 5000 mg/kg BW of EACr did not provoke death in female mice in the acute toxicity setting. For subacute toxicity in rats (male and female), the administration of repetitive doses of 500, 1000, and 2000 mg/kg BW of EACr over a period of 28 days did not cause death in these animals. In females, these doses caused weight loss and a decrease in platelets. The extract reduced the activity of AST in both sexes of rats. The findings also showed that this extract would possess a nephroprotective property in male and female rats. Keywords: Crotalaria retusa, Acute and subacute toxicities, Wistar rat, Swiss mice

Sign in / Sign up

Export Citation Format

Share Document