scholarly journals Effect of Honey Supplementation on Sperm Abnormality in Sperm Preservation of Brek Fish Systomous orphoides

2021 ◽  
Author(s):  
Sri Widiyanti Rahayu Hilia ◽  
Abinawanto ◽  
Anang Hari Kristanto
Keyword(s):  
Sperm Abnormality

scholarly journals PENGARUH AIR REBUSAN AKAR ARU (Caesalpinia bonduc) TERHADAP KUALITAS SPERMA EPIDIDIMIS MENCIT (Mus musculus) : DASAR PENGEMBANGAN OBAT K0NTRASEPSI TRADISIONAL BAGI LAKI-LAKI

2013 ◽  
Vol 13 (2) ◽  
Author(s):  
D. Setiadi Dan S. Bachri
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Mus Musculus ◽  
Treated Group ◽  
Sperm Maturation ◽  
Motile Sperm ◽  
Abnormal Sperm ◽  
Sperm Abnormality ◽  
Boiled Water

AbstrakIndonesia memiliki banyak tumbuhan berpotensi obat salah satunya bisa dijadikan sebagai obatkontrasepsi tradisonal yang biasa digunakan untuk menjarangkan anak atau sterilisasi sepertirebusan akar Caesalpinia bonduc (Aru). Tujuan penelitian untuk mengetahui pengaruh obatkontrasepsi tradisional air rebusan akar aru terhadap kualitas pematangan sperma epididimis mencit(Mus musculus). Mencit dipilih secara acak untuk mewakili 4 kelompok dosis yaitu : 0,0 (K0), 16,0(K1) 24,0 (K2) dan 32,0 (K3) μl/gram berat badan/hari. Setiap kelompok perlakuan dilakukandengan 5 kali replikasi. Perlakuan diberikan melalui oral dengan menggunakan jarum gavage selama10 hari berturut-turut. Pembedahan dilakukan pada hari pertama setelah perlakuan selesai.Pengamatan kualitas sperma epididimis dengan perhitungan produksi sperma, % sperm motil, %sperma hidup, % sperma abnormal,dan rangking keaktifan sperma. Untuk mengetahui ada atautidaknya perbedaan yang bermakna antar perlakuan dalam kelompok dilakukan uji Anava satu arah,bila terdapat perbedaan bermakna dilanjutkan uji BNJ untuk membandingkan angka rata-rata antarkelompok perlakuan. Pemberian air rebusan akar Caesalpinea bonduc pada mencit menunjukanpengaruh perbedaan secara signifikan pada jumlah (%) sperma abnormal antara dosis 0,0 μl dengan24,0 dan 32,0 μl serta antara 16,0 μl dengan 24,0 dan 32,0 μl; Jumlah (%) sperma hidup terdapatperbedaan antara dosis 0,0 μl dengan 16,0 , 24,0 dan 32,0 μl; Jumlah (%) sperma motil terdapatperbedaan antara dosis 0,0 μl dengan 24,0 dan 32,0 μl; Rangking keaktifan sperma terdapatperbedaan antara dosis 0,0 μl dengan 24,0 dan 32,0 μl. Pemberian rebusan akar aru berpengaruhsecara signifikan terhadap peningkatan persen sperma abnormal, dan penuruan persen sperma hidup,motil dan keaktifan.AbstractIndonesia has many species of plants that have potent of medicine, one of them cold be as antraditional contraception medicine ussually used to limit child or sterilizatiom such as root boiledwater of Caesalpinia bonduc. The aims of this study is to know the effect of root boiled water ofCaesalpinia bonduc as traditional contraception medicine on quality of sperm maturation ofepididymis of mice (Mus musculus). Mice were choosed radomly and doses gouped: 0,0 (K0 ), 16,0(K1) 24,0 (K2) and 32,0 (K3) μl/gram body weight/day. Each of group was replicated 5 times.Treatment were given by oral with using gavage needle for ten days. Surgery was carried out on firstday after completing treatments. Examination of epidymis sperm by counting number of spermproduction, percentage of motil, live, abnormal sperm, and rank of sperm motility. In order to knowthe deferences between control and treated group, was used one way anava analysis and analysedvalue for comparing between teratment group. The treatments of root boiled water of Caesalpiniabonduc have effect significantly on percentage of abnormal sperm between dose of 0,0 μl with 24,0and 32,0 μl, also between 16,0 μl with 24,0 and 32,0 μl. Percentage of live sperem is different betweendose of 0,0 μl with 16,0 , 24,0 and 32,0 μl. Percentage of motile sperm is defferent significantlybetween dose of 0,0 μl with 24,0 and 32,0 μl. Meanwhile percentage of motile rank has differencebetween dose of 0,0 μl with 24,0 and 32,0 μl. The treatment of root boiled water of Caesalpiniabonduc has effect signifiantly on increasing percentange of sperm abnormality, decreasingpercentage of life sperm, motile and rank of motile of mice sperm.

scholarly journals The Effect of Genetically Modified Food on Infertility Indices: A Systematic Review Study

2020 ◽  
Vol 2020 ◽  
pp. 1-7 ◽  
Author(s):  
Parisa Keshani ◽  
Mohammad Hossein Sharifi ◽  
Mohammad Reza Heydari ◽  
Hassan Joulaei
Keyword(s):  
Systematic Review ◽  
Adverse Effects ◽  
Sperm Motility ◽  
Genetically Modified ◽  
Sperm Head ◽  
Gm Crops ◽  
Control Group ◽  
Gm Crop ◽  
Gm Plants ◽  
Sperm Abnormality

Background and Objectives. With the increase in the growth of genetically modified (GM) crops, concerns as to the adverse effects of GM crops have risen in the community. The present systematic review seeks to assess the GM plants’ potential impacts on the sperm parameters, including sperm head, sperm motility, sperm abnormality, and fertility index. Materials and Methods. A structured literature search was independently performed by three authors on Scopus, Web of Science, PubMed, and Embase in January 2019. A total of 1467 publications were obtained by the search strategy after eliminating the duplicates. Moreover, the review only included articles written in English language. Other pertinent peer-reviewed publications were chosen (“snowballing”) from the reference lists in the selected publications. To assess the GM crop effects on infertility, experimental studies designed with the control group were selected. On the basis of abstract screening and full-text reviewing, 39 relevant publications were selected, seven of which were used in our review. To access the quality of articles, we used the Cochrane checklist. The collected articles were scored independently by three authors, and the publications with the eligibility criteria were included in our review. No article was excluded due to quality assessment. Results and Conclusion. Our findings indicated that GM products had no adverse effects on infertility indices such as the sperm head, sperm motility, sperm abnormality, and fertility indices. Long-term research still seems to be strongly necessary to ensure that the use of GM plants does not cause any harm to consumers, especially in infertility area.

Safety Evaluation of Zinc Threoninate Chelate

2010 ◽  
Vol 29 (4) ◽  
pp. 372-379 ◽  
Author(s):  
Xiao-bo Hu ◽  
Yi Gong ◽  
Lei Li ◽  
Shao-ping Nie ◽  
Yuan-xing Wang ◽  
...  
Keyword(s):  
Micronucleus Test ◽  
Lethal Dose ◽  
Clinical Signs ◽  
Organ Weight ◽  
Female Rats ◽  
Male Rats ◽  
Repeat Dose ◽  
Pregnant Rats ◽  
Sperm Abnormality ◽  
Daily Dose

The acute toxicity of zinc threoninate chelate was assessed. The oral lethal dose 50% (LD50) was 2710 mg/kg in female rats and 3160 mg/kg in male rats. Genotoxicity was assessed by Ames test in Salmonella typhimurium strains TA97, TA98, TA100, and TA102, by bone marrow mouse micronucleus test and a sperm abnormality test with mice. Thirty-day repeat dose toxicity study was conducted at oral daily doses of 0, 42, 169, and 675 mg/kg in rats. Teratogenicity was assessed at the same daily dose in pregnant rats by gavage. No significant changes in body weight, food consumption, organ weight, relative organ weight, hematology, blood biochemistry, histopathology, behavior, mortality, sperm abnormality, mutagenicity, and micronucleus formation were observed and no clinical signs or adverse effects were detected. Zinc threoninate chelate had no significant teratogenic effect at a daily dose of 42 mg/kg.

scholarly journals Studies on the Genotoxicity Behavior of Silver Nanoparticles in the Presence of Heavy Metal Cadmium Chloride in Mice

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Hanan Ramadan Hamad Mohamed
Keyword(s):  
Heavy Metal ◽  
Dna Damage ◽  
Silver Nanoparticles ◽  
Ag Nanoparticles ◽  
Cadmium Chloride ◽  
Human Life ◽  
Abdominal Cavity ◽  
Sperm Concentration ◽  
Sperm Abnormality ◽  
Polychromatic Erythrocytes

Incredible rapid growth in the nanoparticles applications and development increases the daily human exposure to them but humans are exposed to many other pollutants in addition to nanoparticles that forced us to evaluate the effect of heavy metal cadmium chloride (CdCl2) coinjection on silver nanoparticles induced genotoxic risk in this study. Mice were injected into the abdominal cavity with single dose of Ag nanoparticles (20, 41, and 82 mg/kg) or CdCl2(1.5 mg/kg) either separately or together simultaneously and sacrificed 24 hours later. CdCl2cotreatment enhanced the induced dose-dependent sperm abnormality by Ag nanoparticles different doses as shown by the statistical significant decreases in both sperm concentration and motility and increases in the frequency of abnormal sperms and also potentiated the Ag nanoparticles induced chromosomal and DNA damage indicated by the statistical significant elevations in the frequencies of micronucleated polychromatic erythrocytes (MNPCEs) and DNA damage levels. Moreover, statistical elevations in malondialdehyde level and reductions in catalase activity were observed after CdCl2coinjection with Ag nanoparticles compared with Ag nanoparticles treated groups’ values. Ag nanoparticles induced sperm abnormality, clastogenicity, and genotoxicity were potentiated by heavy metal cadmium coinjection that threatens the human life and increases silver nanoparticles genotoxic risks.

scholarly journals A retrospective study of various factors affecting infertility in Rajasthan, India

2020 ◽  
Vol 9 (7) ◽  
pp. 2910
Author(s):  
Preksha T. Singh ◽  
Saroj Choudhary ◽  
Shreyans D. Singhvi
Keyword(s):  
Retrospective Study ◽  
Reproductive Organs ◽  
Factors Affecting ◽  
Sperm Abnormality ◽  
Ultrasound Sonography ◽  
Early Case ◽  
Psychological Impairment ◽  
One Year ◽  
Biochemical Examination

Background: Infertility is seen fairly common in these days and it cause not only reproductive but also psychological impairment of the parents. Infertility leads to multitude of personal as well as societal repercussions. There are various advances in technology which help the parents suffering from infertility. Infertility is defined as the failure to conceive after one year of regular intercourse in women <35 years not using contraception and after six months in women >35 years.Methods: A retrospective study of 400 females and their husbands’ coming to the department of gynecology, Umaid Hospital, Jodhpur, Rajasthan was undertaken from February 2020 to March 2020. A structural self-constructed questionnaire was used in the study. A well-informed verbal consent was taken by the participants of the study. It had open and closed ended questions. Further, an ultrasound sonography (USG) was performed to check the reproductive organs of the patient, to find the cause of infertility, as well as biochemical examination on male sperm, was performed to find out the sperm abnormality, ejection disorders and other long-term illness.Results: In our study, we found multiple factors affecting infertility issues in females. These factors were- menstrual cycle abnormally, bleeding abnormality and years of active married life. We also found Polycystic ovarian disorder as a major underlying cause of female infertility and sperm abnormality as a major cause of male infertility.Conclusions: Authors recommend frequent female routine checkups to asses early case of infertility and treat it as early as possible.

scholarly journals A High Incidence of Sperm with Cytoplasmic Droplets Affects the Response to Bicarbonate in Preserved Boar Semen

Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2570
Author(s):  
Heiko Henning ◽  
Anne-Marie Luther ◽  
Dagmar Waberski
Keyword(s):  
Calcium Influx ◽  
Specific Response ◽  
Flow Cytometric ◽  
Sperm Abnormality ◽  
High Incidence ◽  
Semen Storage ◽  
Boar Semen ◽  
Calcium Influx Assay

Retained cytoplasmic droplets (CD) are the most frequent sperm abnormality in boar semen. A high incidence of CD is associated with subfertility, but the underlaying reasons are not well understood. The storage of extended semen might augment the adverse effects of CD on essential steps towards fertilization, such as capacitation. The aim of this study was to examine whether the enhanced presence of CD in boar semen influences sperm’s response to the capacitation stimulus bicarbonate during long-term semen storage. Extended semen samples (n = 78) from 13 artificial insemination centers were analyzed using a flow cytometric calcium influx assay. Samples with >15% of CD showed a reduced specific response to bicarbonate and a higher non-specific destabilization after storage for 96 h and subsequent incubation at 38 °C in three variants of Tyrode`s medium (p < 0.05). The size of the bicarbonate-responsive sperm population was inversely correlated with the presence of CD-bearing sperm (r = −0.61, p < 0.01). Samples with ≤15% and samples with >15% of CD did not differ in motility or viability and acrosome integrity during semen storage. In conclusion, incomplete epididymal sperm maturation impairs the in vitro capacitation ability and promotes sperm destabilization in stored boar semen.

scholarly journals Raw Semen Characteristics of Three Different Indonesian Local Roosters

2016 ◽  
Vol 18 (3) ◽  
pp. 165
Author(s):  
Emilia Kamung Hambu ◽  
Raden Iis Arifiantini ◽  
Bambang Purwantara ◽  
Sri Darwati
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Mass Movement ◽  
Sperm Concentration ◽  
Semen Volume ◽  
Sperm Abnormality ◽  
Microscopic Evaluation ◽  
Semen Characteristics ◽  
Live Sperm ◽  
Better Than

Indonesia has agreat variety of roosters, either indigenous type as well as exotic and cross breed. The purpose of this experiment was to study the characteristics of semen from three types of Indonesian local roosters such as Merawang, Kampung and crosses Sentul Kampung with Kedu (SK Kedu). A total of 15 roosters consist of  Merawang roosters,  Kampung, and SK Kedu roosters were 5 each. The semen was collected 3 times a week by dorso-abdominal and cloaca massage method. The parameters evaluation was macroscopic characteristics consist of volume, color, consistency, and pH. Microscopic evaluation of semen such as a mass movement, sperm motility, live sperm, sperm abnormality and sperm concentration. Results of this experiment showed that semen volume of  Merawang  (0.40±0.26 mL) was higher (p<0.05) compare to Kampung (0.24±0.12 mL) or  SK Kedu (0.16±0.10 mL) but no difference on semen color, consistency and semen pH. There were no difference in the mass movement, sperm motility and live sperm as well as on sperm abnormality among three types of roosters.  Sperm  concentration of Merawang (4490 million mL-1) was significantly higher than Kampung (3245 million mL-1) and the SK Kedu roosters (3751 million mL-1). Its was conclude that Merawang roosters had good semen quality better than Kampung and SK Kedu roosters

scholarly journals Factors Affecting The Outcome of in Vitro Fertilization (IVF)

2022 ◽  
Vol 7 (1) ◽  
pp. 444
Author(s):  
Fayka Putri Poempida ◽  
Jimmy Yanuar ◽  
Hamdani Lunardhi ◽  
Samsulhadi Samsulhadi ◽  
Relly Y. Primariawan
Keyword(s):  
In Vitro Fertilization ◽  
Maternal Smoking ◽  
Maternal Age ◽  
Smoking Habits ◽  
P Value ◽  
Maternal Body Mass Index ◽  
Vitro Fertilization ◽  
Sperm Abnormality ◽  
Maternal Bmi

The high prevalence of infertility motivated researchers to find a solution, henceforth In Vitro Fertilization was invented. Factors that affect the outcome of IVF may include sperm analysis, maternal Body Mass Index (BMI), maternal smoking habits, endometriosis, and maternal age. However, there are ongoing debates about the role of said factors regarding the outcome of IVF. The objective of this research is to analyze those factors. This research is a Case-Control study with an analytical observational design. Data were retrieved from patients’ medical records undergoing IVF in Graha Amerta Fertility Clinic from January 2019-October 2020. First, the Chi-Square Test revealed sperm abnormality (p=0.212), Maternal BMI (p=0.427), endometriosis (p=0.067), meaning there was no connection with the outcome of IVF. Simultaneously, maternal age (p=0.037) showed a connection with the outcome of IVF. From the Binary Logistic Regression Test, maternal age 36-40 years old (p=0.044) affects the outcome of IVF significantly. Concurrently maternal BMI, endometriosis, and sperm abnormality have p value>0.05 meaning it is insignificant to the outcome of IVF. This research concluded that sperm abnormality, maternal BMI, and endometriosis do not affect the outcome of IVF. There was no data about maternal smoking habits. Whilst maternal age affects the outcome of IVF. Conclusion: This research concluded that sperm abnormality, maternal BMI, and endometriosis do not affect the outcome of IVF. There was no data about maternal smoking habits. Whilst maternal age affects the outcome of IVF.

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