scholarly journals Studies on the Genotoxicity Behavior of Silver Nanoparticles in the Presence of Heavy Metal Cadmium Chloride in Mice

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Hanan Ramadan Hamad Mohamed
Keyword(s):  
Heavy Metal ◽  
Dna Damage ◽  
Silver Nanoparticles ◽  
Ag Nanoparticles ◽  
Cadmium Chloride ◽  
Human Life ◽  
Abdominal Cavity ◽  
Sperm Concentration ◽  
Sperm Abnormality ◽  
Polychromatic Erythrocytes

Incredible rapid growth in the nanoparticles applications and development increases the daily human exposure to them but humans are exposed to many other pollutants in addition to nanoparticles that forced us to evaluate the effect of heavy metal cadmium chloride (CdCl2) coinjection on silver nanoparticles induced genotoxic risk in this study. Mice were injected into the abdominal cavity with single dose of Ag nanoparticles (20, 41, and 82 mg/kg) or CdCl2(1.5 mg/kg) either separately or together simultaneously and sacrificed 24 hours later. CdCl2cotreatment enhanced the induced dose-dependent sperm abnormality by Ag nanoparticles different doses as shown by the statistical significant decreases in both sperm concentration and motility and increases in the frequency of abnormal sperms and also potentiated the Ag nanoparticles induced chromosomal and DNA damage indicated by the statistical significant elevations in the frequencies of micronucleated polychromatic erythrocytes (MNPCEs) and DNA damage levels. Moreover, statistical elevations in malondialdehyde level and reductions in catalase activity were observed after CdCl2coinjection with Ag nanoparticles compared with Ag nanoparticles treated groups’ values. Ag nanoparticles induced sperm abnormality, clastogenicity, and genotoxicity were potentiated by heavy metal cadmium coinjection that threatens the human life and increases silver nanoparticles genotoxic risks.

scholarly journals Influence of cadmium chloride on the frequency of micronuclei in DA and AO rats

Genetika ◽  
2018 ◽  
Vol 50 (2) ◽  
pp. 705-716
Author(s):  
Slavica Popovic-Bubujuk ◽  
Ninoslav Djelic ◽  
Adrian Muster ◽  
Dragan Kataranovski ◽  
Marko Andjelkovic
Keyword(s):  
Heavy Metal ◽  
Body Weight ◽  
Cadmium Chloride ◽  
Environmental Hazard ◽  
Rat Strains ◽  
Genotoxic Effects ◽  
Experimental Animals ◽  
Polychromatic Erythrocytes ◽  
Phosphate Buffered Saline

Heavy metal cadmium (Cd), a well-known environmental hazard, exerts a number of toxic and genotoxic effects. Experimental animals, Albino Oxford (AO) and Dark August (DA) rat strains, were treated intraperitoneally with three different concentrations of cadmium chloride (CdCl2): 0.5, 1, and 2 mg CdCl2 per kg of body weight, while the control animals received equal volume of sterile phosphate buffered saline. In this investigation individuals of both sexes aged 3, 6 and 12 month were used. Frequency of micronuclei formation was evaluated in polychromatic erythrocytes (PCEs), 24h hours after treatment. The results showed that that cadmium-cloride (CdCl2) exibits the genotoxic effects causing an increase of the frequency of micronuclei depending on concentration, sex, age and strain.

Curcumin-containing Silver Nanoparticles prevent carbon tetrachlorideinduced hepatotoxicity in mice

2020 ◽  
Vol 23 ◽  
Author(s):  
Hossam Ebaid ◽  
Mohamed Habila ◽  
Iftekhar Hassan ◽  
Jameel Al-Tamimi ◽  
Mohamed S. Omar ◽  
...  
Keyword(s):  
Dna Damage ◽  
Silver Nanoparticles ◽  
Nuclear Dna ◽  
Liquid Paraffin ◽  
Tail Length ◽  
Hepatic Tissue ◽  
Tissue Destruction ◽  
Group 2 ◽  
The Impact

Background: Hepatotoxicity remains an important clinical challenge. Hepatotoxicity observed in response to toxins and hazardous chemicals may be alleviated by delivery of the curcumin in silver nanoparticles (AgNPs-curcumin). In this study, we examined the impact of AgNPs-curcumin in a mouse model of carbon tetrachloride (CCl4)-induced hepatic injury. Methods: Male C57BL/6 mice were divided into three groups (n=8 per group). Mice in group 1 were treated with vehicle control alone, while mice in Group 2 received a single intraperitoneal injection of 1 ml/kg CCl4 in liquid paraffin (1:1 v/v). Mice in group 3 were treated with 2.5 mg/kg AgNPs-curcumin twice per week for three weeks after the CCl4 challenge. Results: Administration of CCL4 resulted in oxidative dysregulation, including significant reductions in reduced glutathione and concomitant elevations in the level of malondialdehyde (MDA). CCL4 challenge also resulted in elevated levels of serum aspartate transaminase (AST) and alanine transaminase (ALT); these findings were associated with the destruction of hepatic tissues. Treatment with AgNPs-curcumin prevented oxidative imbalance, hepatic dysfunction, and tissue destruction. A comet assay revealed that CCl4 challenge resulted in significant DNA damage as documented by a 70% increase in nuclear DNA tail-length; treatment with AgNPs-curcumin inhibited the CCL4-mediated increase in nuclear DNA tail-length by 34%. Conclusion: Administration of AgNPs-curcumin resulted in significant antioxidant activity in vivo. This agent has the potential to prevent the hepatic tissue destruction and DNA damage that results from direct exposure to CCL4.

A brief Survey of Assessment models to Predict stress Level of Heavy Metals in Soils

2020 ◽  
Vol 13 ◽  
Author(s):  
Sangeetha Annam ◽  
Anshu Singla
Keyword(s):  
Economic Growth ◽  
Heavy Metals ◽  
Heavy Metal ◽  
Ecological Risk ◽  
Stress Level ◽  
Metal Contamination ◽  
Heavy Metal Contamination ◽  
Human Life ◽  
High Stress ◽  
Heavy Metal Stress

Abstract: Soil is a major and important natural resource, which not only supports human life but also furnish commodities for ecological and economic growth. Ecological risk has posed a serious threat to the ecosystem by the degradation of soil. The high-stress level of heavy metals like chromium, copper, cadmium, etc. produce ecological risks which include: decrease in the fertility of the soil; reduction in crop yield & degradation of metabolism of living beings, and hence ecological health. The ecological risk associated, demands the assessment of heavy metal stress levels in soils. As the rate of stress level of heavy metals is exponentially increasing in recent times, it is apparent to assess or predict heavy metal contamination in soil. The assessment will help the concerned authorities to take corrective as well as preventive measures to enhance the ecological and hence economic growth. This study reviews the efficient assessment models to predict soil heavy metal contamination.

scholarly journals Anticancer Potential of Biogenic Silver Nanoparticles: A Mechanistic Study

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 707
Author(s):  
Mohd Shahnawaz Khan ◽  
Alya Alomari ◽  
Shams Tabrez ◽  
Iftekhar Hassan ◽  
Rizwan Wahab ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Cell Lines ◽  
Human Life ◽  
Mechanistic Study ◽  
Dependent Manner ◽  
Rt Pcr ◽  
Cell Viability Assay ◽  
Hct 116 ◽  
Mcf 7

The continuous loss of human life due to the paucity of effective drugs against different forms of cancer demands a better/noble therapeutic approach. One possible way could be the use of nanostructures-based treatment methods. In the current piece of work, we have synthesized silver nanoparticles (AgNPs) using plant (Heliotropiumbacciferum) extract using AgNO3 as starting materials. The size, shape, and structure of synthesized AgNPs were confirmed by various spectroscopy and microscopic techniques. The average size of biosynthesized AgNPs was found to be in the range of 15 nm. The anticancer potential of these AgNPs was evaluated by a battery of tests such as MTT, scratch, and comet assays in breast (MCF-7) and colorectal (HCT-116) cancer models. The toxicity of AgNPs towards cancer cells was confirmed by the expression pattern of apoptotic (p53, Bax, caspase-3) and antiapoptotic (BCl-2) genes by RT-PCR. The cell viability assay showed an IC50 value of 5.44 and 9.54 µg/mL for AgNPs in MCF-7 and HCT-116 cell lines respectively. We also observed cell migration inhibiting potential of AgNPs in a concentration-dependent manner in MCF-7 cell lines. A tremendous rise (150–250%) in the production of ROS was observed as a result of AgNPs treatment compared with control. Moreover, the RT-PCR results indicated the difference in expression levels of pro/antiapoptotic proteins in both cancer cells. All these results indicate that cell death observed by us is mediated by ROS production, which might have altered the cellular redox status. Collectively, we report the antimetastasis potential of biogenic synthesized AgNPs against breast and colorectal cancers. The biogenic synthesis of AgNPs seems to be a promising anticancer therapy with greater efficacy against the studied cell lines.

scholarly journals Research on heavy metal level and co-occurrence network in typical ecological fragile area

2021 ◽  
Author(s):  
Yiwei Zhao ◽  
Liangmin Gao ◽  
Fugeng Zha ◽  
Xiaoqing Chen ◽  
Xiaofang Zhou ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Human Factors ◽  
Human Life ◽  
Mining Area ◽  
Parent Material ◽  
Control Value ◽  
Pollution Levels ◽  
Soil Parent Material ◽  
Sampling Points

AbstractDue to the special sensitivity of typical ecologically fragile areas, a series of human life, mining, and other activities have a greater impact on the environment. In this study, three coal mines in Ordos City on the Loess Plateau were selected as the study area, and the pollution levels of heavy metals in the area were studied by measuring As, Hg, Cr, Cd, Cu, Ni, and Pb in the soil of 131 sampling points. Combined with the concept of “co-occurrence network” in biology, the level of heavy metals in soil was studied using geostatistics and remote sensing databases. The results showed that the concentrations of Hg, Cr, Ni, Cu, and Pb in more than half of the sampling points were higher than the local environmental background value, but did not exceed the risk control value specified by China, indicating that human factors have a greater influence, while Cd and As elements are mainly affected Soil parent material and human factors influence. Heavy metal elements have nothing to do with clay and silt but have an obvious correlation with gravel. Cd, Pb, As and Ni, Cd, Cr are all positively correlated, and different heavy metals are in space The distribution also reflects the autocorrelation, mainly concentrated in the northeast of the TS mining area and the middle of the PS mining area.

Shape and stability of silver nanoparticles and their dependence on the conditions of preparation.

2012 ◽  
Vol 1371 ◽  
Author(s):  
M.I. Hernández-Castillo ◽  
O. Zaca-Moran ◽  
P. Zaca-Moran ◽  
M. Rojas-López ◽  
V.L. Gayou ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Reaction Time ◽  
Ag Nanoparticles ◽  
Reduction Procedure ◽  
Visible Spectrophotometry ◽  
Logical Sequence ◽  
Uv Visible ◽  
Citrate Reduction ◽  
Shape And Size

ABSTRACTBy using the citrate reduction procedure we have synthesized Ag nanoparticles, applying several conditions of preparation, being after characterized by UV-visible spectrophotometry. Following a logical sequence, the starting experiment was realized varying the reaction time, after that it was varied the concentration of the reductor agent, and finally it was varied the volume of the reductor agent. According to this methodology, TEM measurements show that firstly we have nanostructures with different shape and size, whereas in the last part of the experiment we have Ag nanoparticles with homogeneous shape and size.

Facile Synthesis of Silver Nanoparticles by Chemical Reduction Route

2013 ◽  
Vol 756 ◽  
pp. 99-105
Author(s):  
Rajasingam Ratnamalar ◽  
Mustapha Mariatti ◽  
Zulkifli Ahmad ◽  
Sharif Zein Sharif Hussein
Keyword(s):  
Silver Nanoparticles ◽  
Ag Nanoparticles ◽  
Chemical Reduction ◽  
Facile Synthesis ◽  
Cubic Lattice ◽  
Vis Spectroscopy ◽  
Simple Chemical ◽  
The Face ◽  
Fine Control ◽  
Xrd Patterns

This work reports a simple chemical reduction route for the preparation of uniformed Ag nanoparticles whereby a fine control over the sizes of the Ag nanoparticles was studied by varying the concentrations of the reducing agents used. In characterization, UV-Vis spectroscopy showed the changes in optical properties of the Ag nanoparticles with regards to their sizes, where as the XRD patterns of the synthesized Ag nanoparticles confirmed the distinct peaks approximately at 2θ = 38.1°, 44.3°, 64.4°, 77.4°, and 81.5 representing Bragg’s reflections from (111), (200), (220), (311), and (222) planes of the face centred cubic lattice phase. This route of synthesis is feasible to produce Ag nanoparticles with diameters in the range of 30-45 nm.

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