scholarly journals In vitro antibacterial activity of garlic (Allium sativum) and ginger (Zingiber officinale) aqueous extracts against isolates of Brucella abortus

2021 ◽  
Vol 6 (1) ◽  
pp. 064-070
Author(s):  
Ayda Ali Khalifa ◽  
Ali A ElGadal ◽  
Firooz M Youssif ◽  
Mutaman A Kehail
Keyword(s):  
Antibacterial Activity ◽  
Brucella Abortus ◽  
Pathogenic Bacteria ◽  
Allium Sativum ◽  
Zingiber Officinale ◽  
Garlic Extract ◽  
Diffusion Method ◽  
Dilution Method ◽  
Sensitivity Pattern

Microbial resistance to antibiotics has become a problem plaguing the world. Currently, interest has been focused on exploring antimicrobial properties of plants and herbs. This work aim to evaluate the antibacterial activity of garlic (Allium sativum) bulbs and ginger (Zingiber officinale) rhizome on Brucella abortus isolates. Some concentrations of garlic and ginger extracts were tested for their antibacterial activity against B. abortus isolate brought from Central Veterinary Research Laboratory (CVRL), Soba, using well diffusion method. Moreover, minimum inhibitory concentration (MIC) and minimum lethal concentration (MLC) of garlic and ginger were tested using broth dilution method. Sensitivity pattern of the conventional antibacterial against common pathogenic bacteria was tested using disc diffusion method. Aqueous extract of ginger produced dose-dependent increase in the zone of inhibition at a concentration of 15% and higher, whereas the garlic extract produced inhibition zone at a concentration of 5% and higher, i.e. B. abortus isolate showed relatively high sensitivity toward garlic extract than ginger which required a more concentrated extract to kill or inhibit B. abortus isolate that brought from (CVRL), Soba, Khartoum, Sudan. Further studies are needed to find out the efficacy, safety, and kinetic data of their active ingredients.

Investigation for New Inhibitors of UDP-N-Acetylglucosamine Enolpyruvyl Transferase (MurA) by Virtual Screening with Antibacterial Assessment

2020 ◽  
Vol 16 ◽  
Author(s):  
Ilham Boulhissa ◽  
Abdelouahab Chikhi ◽  
Abderrahmane Bensegueni ◽  
Mohammad Ahmad Ghattas ◽  
El Hassen Mokrani ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Virtual Screening ◽  
Pathogenic Bacteria ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Dilution Method ◽  
Chemical Library ◽  
Broth Dilution

Background: View to its interesting role in the peptidoglycan biosynthesis pathway the enzyme UDP-N- acetylglucosamine enolpyruvyl transferase is an attractive target to develop new antibacterial agents, it catalyzes the first key step of this pathway and its inhibition leads to the bacterial cell death. Fosfomycin is known as the natural inhibitor of MurA. Objective: Call new inhibitors of MurA by virtual screening of different chemical compounds libraries, and test the best scored “virtual hits” against three pathogenic bacteria: Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. Methods: A Virtual screening of the structural analogues of fosfomycin downloaded from PubChem database was performed on one side and of the French National Chemical Library as well as using ZINC database to identify new structures different from fosfomycin on the other, FlexX was the software used for this study. The antibacterial testing was divided into methods: disk diffusion and broth dilution. Results: A set of virtual hits was found with better energy score than that of fosfomycin, seven between them were tested in vitro. Therefore, disk diffusion method explored four compounds exhibited antibacterial activity: CID-21680357 (fosfomycin analogue), AB-00005001, ZINC04658565, and ZINC901335. The testing was continued by broth dilution method for both compounds CID-21680357 and ZINC901335 to determine their minimum inhibitory concentrations, and ZINC901335 had the best value with 457µg/ml against Staphylococcus aureus. Conclusion: Four compounds were found and proven in silico and in vitro to have antibacterial activity: CID-21680357, AB-00005001, ZINC04658565, and ZINC901335.

scholarly journals Herbominerals and Antibacterial Activities of Allium sativum L Extracts on Pathogenic Bacteria Causing Meningitis in Sub-Saharan Africa, Zaria, Kaduna State, Nigeria

2020 ◽  
pp. 1-15
Author(s):  
Kokori Bajeh Tijani ◽  
Danjuma Nuhu Muhammed ◽  
Janet I. Ejiofor ◽  
Busayo Olayinka ◽  
Abdullahi Attah Alfa
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Meningitis ◽  
Pathogenic Bacteria ◽  
Allium Sativum ◽  
Sub Saharan Africa ◽  
Clinical Isolates ◽  
Dilution Method ◽  
Distilled Water ◽  
Phytochemical Screening

Bacterial Meningitis (BM) is the most common serious infection of the central nervous system (brain and spinal cord). This research aims to determine the mineral composition and to evaluate the in vitro antibacterial activity of the Juice Extract of Allium Sativum, Ethanolic Extract of Allium Sativum and Aqueous Extract of Allium sativum (JEAS, EEAS and AEAS). The collected bulbs of A. sativum (600 g) were washed and air dried under shade for 2 hours and the dry scaly outer covering was peeled-off to obtain the fresh garlic cloves which were then divided into three parts of 200 g each. These three portions were crushed separately for cold extraction. The first portion was homogenized and poured into a muslin cloth to squeeze out the juice, while second and third portions were homogenized and submerged into 500 ml of 96% ethanol and 500 ml of distilled water respectively for 24 hours and both filtered after thorough shaking. The antibacterial activity of bulbs of A. sativum juice, ethanolic and aqueous (JEAS, EEAS and AEAS) extracts as folkloric medicine against clinical isolates were determined using Agar well diffusion and broth dilution method. Distilled water, concentrated nitric acid (HNO3) and hydrochloric acid (HCl) were used to digest the extract, which was then heated in water bath at 90ºC and filtered to obtain the filtrate for the analytical studies for A. sativum nutritional composition and zeolite herbominerals. The micro-herbominerals with their proximate values observed pharmacologic of Silver, Manganese, Zinc, Iron and Selenium; which has biocidal properties as well as immune system to cushioning the challenges of the BM pathogens. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and phytochemical screening of the extracts were evaluated. The results obtained showed that the juice and ethanolic extracts were potent, inhibiting the growth of clinical isolates with zone of inhibition ranging from 14-36 mm. The extracts inhibited bacterial isolates in concentration dependant manner with MICs ranging 0.02-15 mg/ml and MBCs 0.04-5 mg/ml. Phytochemical screening of the extracts revealed the presence of alkaloids, flavonoids, anthraquinone, carbohydrates, fats and oils, steroidal ring, saponins and terpenoids. This experimental investigation has provided the scientific validation basis for the ethnomedical use of A. sativum as a remedy to treat bacterial meningitis locally as anti-infectious agent.

scholarly journals Antibiotic sensitivity and in vitro antimicrobial activity of plant extracts to pseudomonas fluorescens isolates collected from diseased fish

1970 ◽  
Vol 1 (4) ◽  
pp. 82-88 ◽  
Author(s):  
MJ Foysal ◽  
MM Rahman ◽  
M Alam
Keyword(s):  
Antibacterial Activity ◽  
Pseudomonas Fluorescens ◽  
Plant Extracts ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Leaf Extracts ◽  
Sensitivity Pattern ◽  
Medicinal Plant Extracts

Studies were conducted to identify Pseudomonas fluorescens isolates from a collection of bacteria isolated from bacterial haemorrhagic septicaemia infected carp and catfish, evaluate their antibiotic sensitivity pattern and screen the antibacterial activity of some medicinal plant extracts against the isolates.. A total of 10 isolates were identified as P. fluorescens by morphological, physiological and biochemical tests. In vitro antibiotic sensitivity test of the P. fluorescens isolates were conducted by disc diffusion method for seven antibiotics where, all of the isolates were found to be sensitive only against streptomycin and gentamycin but, most of the isolates (80%) were found resistant to chloramphenicol (C). Moreover, eighty percent of the isolates showed resistance to multiple antibiotics. A total of 118 plant extracts were screened for their antibacterial activity against the P. fluorescens isolates where the isolates exhibited sensitivity to 30 samples. Leaf extracts of Tamarindus indicus, Terminalia chebula, Citrus aurantifolia, Eugenia caryophyllata and Spondias pinnata were found to inhibit the growth of all of the P. fluorescens isolates. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9733 IJNS 2011 1(4): 82-88

scholarly journals IN VITRO ANTAGONISTIC ACTIVITIES OF INDONESIAN MARINE SPONGE AAPTOS AAPTOS AND CALLYSPONGIA PSEUDORETICULATA EXTRACTS AND THEIR TOXICITY AGAINST Vibrio spp.

2011 ◽  
Vol 6 (2) ◽  
pp. 173 ◽  
Author(s):  
Rosmiati Rosmiati ◽  
Habsah Mohamad ◽  
Tengku Sifzizul Tengku Muhammad ◽  
Najiah Musa ◽  
Aziz Ahmad ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Pathogenic Bacteria ◽  
Vibrio Harveyi ◽  
Diffusion Method ◽  
Larval Rearing ◽  
Butanol Extract ◽  
Vibrio Spp ◽  
Mic Value

Vibriosis is one of diseases which often results in mass mortality of Penaeus monodon larval rearing systems. It attacks shrimp of all stages in zoea, mysis and shrimp postlarva stage. This disease is caused by Vibrio spp, particularly Vibrio harveyi (a luminescent bacterium). Several kinds of antibiotics and chemical material have been used to overcome the disease but they have side effects to environment and human. The searching of bioactive compounds as an alternative treatment has been done for multi purposes. In this study diethyl eter, butanol and aqueous extract of Indonesian sponges Aaptos aaptos and Callyspongia pseudoreticulata were tested for in vitro activity against Vibrio spp. and Vibrio harveyi by using disc diffusion method. The result showed that all extracts of Aaptos aaptos gave a positive antibacterial activity towards those pathogenic bacteria. Meanwhile, only butanol extract of Callyspongia pseudoreticulata obtained to exhibit an antibacterial activity on those pathogenic bacteria. The strong anti-vibrio activity were shown by butanol and aqueous extract of Aaptos aaptos with the minimum inhibitory concentration (MIC) value of 0.313 and 0.625 mg/mL, respectively. Whilst, the butanol extract of Callyspongia pseudoreticulata indicated a low antibacterial activity with the MIC value of 10 mg/mL. Toxicity of those active extracts was evaluated by Brine Shrimp Lethality Test (BST). Interestingly, butanol and aqueous extracts of Aaptos aaptos did not show any toxic effect in Artemia salina larvae up to 8 x MIC (2.504 mg/mL and 5.000 mg/mL). It is the first report for the anti-vibr io activity of both Aaptos aaptos and Callyspongia pseudoreticulata. This results suggest that Aaptos aaptos has a potential to be used as a source of alternative compound to vibriosis prevention for mariculture.

scholarly journals IN VITRO EVALUATION OF ANTIBACTERIAL ACTIVITY OF GARLIC ALLIUM SATIVUM AGAINST POULTRY PATHOGENS AND EFFECT OF GARLIC SUPPLEMENTATION ON DUCKLING GROWTH PERFORMANCE

2019 ◽  
Vol 57 (3B) ◽  
pp. 9 ◽  
Author(s):  
Tien Cuong Nguyen ◽  
Thi Anh Phuong Chu ◽  
Hai Van Nguyen
Keyword(s):  
Antibacterial Activity ◽  
Growth Performance ◽  
Pathogenic Bacteria ◽  
Allium Sativum ◽  
Basal Diet ◽  
Feed Consumption ◽  
Poultry Production ◽  
Feed Conversion ◽  
Garlic Powder

ABSTRACT-QMFS2019Poultry production provides source of protein and contributes an important income for Vietnamese farmers. Among the poultry in Vietnam, ducks account for 27.3% of head of poultry and even 55.7% in Mekong Delta region. Along with the development of rearing ducks, bacterial, viral and fungal diseases occurring in the two last decades induced bad effect for poultry producer. Escherichia coli, Salmonella enterica, Streptococcal or Pasteurella act as major pathogenic bacteria in duck. The aims of this study were to investigate the antibacterial activity of garlic Allium sativum against E. coli, Staphylococcus aureus, Salmonella Typhimurium and to evaluate the effect of garlic on growth performance of duck from 1-28 old-days. The results indicated that fresh garlic and dried garlic powder showed inhibitory effect against pathogenic tested strains from 2% and 4% w/v, respectively. The inhibition zones and the minimal inhibitory concentration (MIC) values of garlic extract ranged from 11.3-28.3 mm and 0.02-0.2 g/ml, respectively. After 28 days of diet with garlic supplemented, D3 (2% of fresh garlic in water) showed significantly different in weight gain (WG), feed conversion ratio (FCR), protein efficiency ratio (PER), average daily weight (ADW); whereas, D2 (2% of garlic powder in basal diet) only possessed a difference significant in feed consumption (FC) compared to the D1 (control without garlic supplementation). The obtained results demonstrated the potential of garlic application in poultry production.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KAPULAGA JAWA (Amomum compactum Soland. ex Maton) TERHADAP Streptococcus pyogenes

EKOLOGIA ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 31-39
Author(s):  
Oom Komala ◽  
. Ismanto ◽  
Muhammad Alan Maulana
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Dilution Method ◽  
Natural Treatment ◽  
Amomum Compactum

Streptococcus pyogenes is one of the pathogenic bacteria that causes pharyngitis. Natural treatment to overcome these problems is to use cardamom seeds. The purpose of this study was to test the antibacterial activity, determine the concentration of inhibitory zone and phytochemical compounds from  ethanol 96% extract of Java cardamom seeds (Amomum compactum Soland. Ex Maton) against Streptococcus pyogenes. The method is used   solid dilution and paper disc diffusion method. The solid dilution method is used for the Minimum Inhibitory Concentration (MIC) test with a concentration of 1.25%, 2.5%, 5% and 7.5% while the paper diffusion method is used for the Inhibition zone Diameter (IZD)  using five treatments namely three concentrations of ethanol 96% extract of Java cardamom seeds (7.5%, 10% and 12%), one positive control of amoxicillin 0.01 mg/mL and one negative control of sterile distilled water. IZD data were  analyzed using ANOVA with a confidence level of 95% and α = 0.05 and Duncan's further tests to determine differences between treatments. The results showed that the MIC  was at a concentration of 7.5% while for the IZD test which had the highest activity there was a concentration of 12% with an average inhibition diameter of 12.03 ± 0.14 mm. In addition, ethanol 96% extract of Java cardamom seeds contain alkaloids, flavonoids, terpenoids and tannins which function in antibacterial activity.

scholarly journals Evaluation of Antibacterial Activity of Some Traditionally Used Medicinal Plants against Human Pathogenic Bacteria

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Bishnu P. Marasini ◽  
Pankaj Baral ◽  
Pratibha Aryal ◽  
Kashi R. Ghimire ◽  
Sanjiv Neupane ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Inhibitory Action ◽  
Pathogenic Bacteria ◽  
Cynodon Dactylon ◽  
Bacterial Species ◽  
Dilution Method ◽  
Chloroform Fraction ◽  
Ethanolic Extracts

The worldwide increase of multidrug resistance in both community- and health-care associated bacterial infections has impaired the current antimicrobial therapy, warranting the search for other alternatives. We aimed to find thein vitroantibacterial activity of ethanolic extracts of 16 different traditionally used medicinal plants of Nepal against 13 clinical and 2 reference bacterial species using microbroth dilution method. The evaluated plants species were found to exert a range ofin vitrogrowth inhibitory action against the tested bacterial species, andCynodon dactylonwas found to exhibit moderate inhibitory action against 13 bacterial species including methicillin-resistantStaphylococcus aureus, imipenem-resistantPseudomonas aeruginosa, multidrug-resistantSalmonella typhi, andS. typhimurium. The minimum inhibitory concentration (MIC) values of tested ethanolic extracts were found from 31 to >25,000 μg/mL. Notably, ethanolic extracts ofCinnamomum camphora, Curculigo orchioides, andCurcuma longaexhibited the highest antibacterial activity againstS. pyogeneswith a MIC of 49, 49, and 195 μg/mL, respectively; whereas chloroform fraction ofCynodon dactylonexhibited best antibacterial activity againstS. aureuswith a MIC of 31 μg/mL. Among all,C. dactylon, C. camphora, C. orchioides, andC. longaplant extracts displayed a potential antibacterial activity of MIC < 100 μg/mL.

scholarly journals Synthesis and In Vitro Antibacterial Activity of Quaternized 10-Methoxycanthin-6-one Derivatives

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1553 ◽  
Author(s):  
Na Li ◽  
Dan Liu ◽  
Jiang-Kun Dai ◽  
Jin-Yi Wang ◽  
Jun-Ru Wang
Keyword(s):  
Antibacterial Activity ◽  
Seed Germination ◽  
Pathogenic Bacteria ◽  
Dilution Method ◽  
Highly Active ◽  
Structure Activity ◽  
In Vitro Antibacterial Activity ◽  
Potential Activity ◽  
Low Cytotoxicity

Background: Based on our previous work, we found that 10-methoxycanthin-6-one displayed potential antibacterial activity and quaternization was an available method for increasing the antibacterial activity. Here, we explored the antibacterial activity of quaternized 10-methoxy canthin-6-one derivatives. Methods and Results: Twenty-two new 3-N-benzylated 10-methoxy canthin-6-ones were designed and synthesized through quaternization reaction. The in vitro antibacterial activity against three bacteria was evaluated by the double dilution method. Moreover, the structure–activity relationships (SARs) were carefully summarized in order to guide the development of antibacterial canthin-6-one agents. Two highly active compounds (6p and 6t) displayed 8-fold superiority (MIC = 3.91 µg/mL) against agricultural pathogenic bacteria R. solanacearum and P. syringae compared to agrochemical streptomycin sulfate, and showed potential activity against B. cereus. Moreover, these two compounds exhibited good “drug-like” properties, low cytotoxicity, and no inhibition on seed germination. Conclusions: This work provides two new effective quaternized canthin-6-one derivatives as candidate bactericide, promoting the development of natural-sourced bactericides and preservatives.

scholarly journals Synthesis and in vitro Antibacterial Activity of Quaternization 10-Methoxycanthin-6-one Derivatives

2019 ◽  
Author(s):  
Na Li ◽  
Jiang-Kun Dai ◽  
Dan Liu ◽  
Jin-Yi Wang ◽  
Jun-Ru Wang
Keyword(s):  
Antibacterial Activity ◽  
Natural Products ◽  
Minimum Inhibitory Concentration ◽  
Pathogenic Bacteria ◽  
Antibacterial Agents ◽  
Inhibitory Concentration ◽  
Dilution Method ◽  
In Vitro Antibacterial Activity

Natural products are an important source of antibacterial agents. Canthin-6-one alkaloids have displayed potential antibacterial activity based on our previous work. In order to improve the activity, twenty-two new 3-N-benzylated 10-methoxy canthin-6-ones were designed and synthesized through quaternization reaction. The in vitro antibacterial activity against three bacteria was evaluated by double dilution method. Four compounds (6f, 6i, 6p and 6t) displayed 2-fold superiority (minimum inhibitory concentration (MIC) = 3.91 &micro;g/mL) against agricultural pathogenic bacteria R. solanacearum and P. syringae than agrochemical propineb. Moreover, the structure&ndash;activity relationships (SARs) were also carefully summarized in order to guide the development of antibacterial canthin-6-one agents.

In-vitro evaluation of cisplatin nanoparticles encompass natural polymer

2020 ◽  
Vol 3 (1) ◽  
pp. 1-6
Author(s):  
Bhavani J ◽  
Sunil Kumar Prajapati ◽  
Ravichandran S
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Research Work ◽  
Dilution Method ◽  
Bacterial Strains ◽  
Active Components ◽  
Synthetic Drugs

Opportunistic bacterial infections are common in the various parts of human body. In recent years bacterial species have shown resistance against a number of synthetic drugs. This study measured the antibacterial activity of bacterial strains against five common pathogenic bacteria related strains. Cup plate method and two fold serial dilution method were used to evaluated by antibacterial activity by the help of different bacterial related strains. The results revealed that Cisplatin (CIP) using natural as a polymer showed a minimum inhibitory concentration (MIC) at 250 mg/ml to 500 mg/ml of the broth against all bacterial strains. CIP using natural as a polymer was prepared different doses1000 μg/ml and 2000 μg/ ml and measured zone of inhibition dose dementedly reduced when compared to standard. The CIP using natural as a polymer exhibited strong anti-bacterial activity against five different species of bacteria and this may be attributed to various active components. Our research work has been indicated Nanoparticles containing CIP using natural as a polymer formulated for the enhanced anti-cancer activity through antimicrobial mechanism. 

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